Skin care composition with effects of shrinking pores, tendering skin and resisting wrinkles as well as preparation method and application of skin care composition

Abstract

The invention discloses a skin care composition with effects of shrinking pores, tendering skin and resisting wrinkles as well as a preparation method and application of the skin care composition, and particularly belongs to the technical field of daily cosmetics. The composition is prepared from the following components in parts by mass: 5 to 8 parts of astragalus membranaceus root extract, 0.15 to 0.2 part of raspberry ketone glucoside, 3 to 5 parts of gloiopeltis furcata extract, 0.8 to 1 part of pyracantha fortuneana fruit extract and 20 to 30 parts of bifidus yeast fermentation product filtrate, and the components are extracted by a specific process. The composition provided by the invention has the effects of shrinking pores, resisting wrinkles, tightening skin, controlling oil and the like, and can be used for preparing various skin care products such as essence, emulsion and the like.

Description

Technical Field

[0001] The present invention belongs to the technical field of daily cosmetics, and in particular relates to a skin care composition with pore-shrinking, skin-rejuvenating and anti-wrinkle effects, as well as a preparation method and application thereof. Background Art

[0002] Studies have found that the causes of enlarged pores are: 1. Excessive secretion of sebum causes the reproduction of lipophilic microorganisms, which causes the hair follicles to be blocked, leading to enlarged pores; 2. The loss of skin collagen leads to insufficient skin firmness, which in turn causes the skin to show enlarged pores.

[0003] While existing products offer diverse benefits, single-ingredient products often have limited effectiveness and struggle to meet demand. For example, some anti-wrinkle products primarily target collagen loss, but are ineffective at controlling oil and addressing problems like enlarged pores. Furthermore, some products suffer from incompatible ingredients, significantly reducing their effectiveness.

[0004] Therefore, there is an urgent need to develop a skin care composition that integrates multiple functions and synergistically enhances the effectiveness of ingredients to meet the market demand for high-quality, comprehensive skin care products. Summary of the Invention

[0005] In view of the deficiencies of the prior art, the purpose of the present invention is to provide a skin care composition and its preparation method and application, which can reduce skin lipid synthesis, thereby reducing pore blockage and tightening pores, while promoting collagen synthesis, firming the skin, and rejuvenating and anti-wrinkle.

[0006] To achieve the above objectives, the present invention discloses the following technical solutions:

[0007] In a first aspect, the present invention provides a skin care composition, comprising the following components in parts by mass:

[0008]

[0009] Preferably, the preparation method of the Astragalus membranaceus root extract comprises the following steps:

[0010] Step 1-1. The dried Astragalus membranaceus root was placed in a grinder and pulverized through a 30-mesh sieve to obtain Astragalus membranaceus root powder;

[0011] Step 1-2. Astragalus membranaceus root powder and deionized water are mixed at a mass volume ratio of 1:20-30 g / mL, and ultrasonically treated at a frequency of 25-30 kHz and 50-60°C for 20-30 min. After ultrasonic treatment, the mixture is kept at 50-60°C for another 12-16 hours, and passed through a 300-400 mesh sieve to obtain a crude extract;

[0012] Step 1-3. The crude extract is placed in a vacuum concentrator and concentrated to 1 / 3 of the original volume at 30-50 kPa and 60-70° C. to obtain the Astragalus membranaceus root extract.

[0013] Preferably, the preparation method of the seaweed extract comprises the following steps:

[0014] Step 2-1. Take a certain amount of seaweed and deionized water at a volume mass ratio of 1: 20-30g / mL and mix them. Crush the wall at 20000-30000r / min for 10-15min to obtain a seaweed homogenate.

[0015] Step 2-2. The seaweed homogenate was heated to 70-80 ° C and kept warm for 50-60min. The temperature was lowered to 25-35 ° C and centrifuged to obtain a supernatant. A 95v / v% ethanol aqueous solution was added to the supernatant, mixed and soaked for 24-26h, and centrifuged to obtain a precipitate.

[0016] Step 2-3. The precipitate obtained by separation in step 2-2 is placed in an oven at 50-60° C. and dried until there is no alcohol smell, thereby obtaining the seaweed extract.

[0017] Preferably, the preparation method of the Pyracantha fortuneana fruit extract comprises the following steps:

[0018] Step 3-1. Take a certain amount of dried Pyracantha fruit and place it in a grinder and grind it through a 60-mesh sieve to obtain Pyracantha fruit powder. Moisten the Pyracantha fruit powder with deionized water to obtain a water content of 40-50% to obtain a solid fermentation matrix, which is sterilized for standby use.

[0019] Step 3-2: Take the viable cell count ≥ 1×10 8 The bacterial liquid of Saccharomyces cerevisiae with a CFU / mL is inoculated into the solid fermentation substrate by spraying at 15-20% of the mass of the fermentation substrate, and cultured at 28-30°C, a ventilation rate of 0.5-0.7 L / min·kg, and a stirring speed of 3-7 r / min for 72-80 hours to obtain a fermentation material;

[0020] Step 3-3. Mix the fermentation material with a 70-80 v / v% ethanol aqueous solution at a ratio of 1:15-20 g / mL, extract under reflux at 70-80°C for 3 times, each time for 1-1.2 hours, combine the extraction filtrates, concentrate the filtrates under reduced pressure to 1 / 3 of the original volume, and finally vacuum dry them to a water content of ≤10% to obtain a Pyracantha fruit extract.

[0021] In a second aspect, the present invention provides a use of the skin care composition described in the first aspect in preparing a skin care product with anti-wrinkle, firming and oil-control effects.

[0022] In a third aspect, the present invention provides an essence having the effects of shrinking pores, rejuvenating the skin and resisting wrinkles, wherein the essence contains the skin care composition according to the first aspect;

[0023] Preferably, the amount of the skin care composition added to the essence is 5-10 wt %.

[0024] More preferably, the essence further contains a preservative, an antioxidant, a moisturizer and a solvent.

[0025] In a fourth aspect, the present invention provides a method for preparing the essence according to the third aspect, the preparation method comprising the following steps:

[0026] The skin care composition, preservative, antioxidant, moisturizer and solvent are mixed and stirred evenly to obtain the essence.

[0027] In the present invention:

[0028] The Astragalus membranaceus root extract obtained by water extraction contains active ingredients such as polysaccharides, flavonoids, saponins, amino acids, etc., which have the effects of whitening and removing spots, anti-aging, antioxidant and anti-inflammatory.

[0029] Raspberry ketone glucoside has a strong antioxidant effect, can effectively scavenge free radicals, and reduce oxidative damage to skin cells. At the same time, it has a strong inhibitory ability on tyrosinase and can effectively inhibit the production of melanin. Raspberry ketone glucoside has been less studied in terms of anti-inflammatory and oil control, but some studies have found that when raspberry ketone is used in combination with anti-inflammatory agents, it promotes the anti-inflammatory effect and bioavailability of anti-inflammatory agents. Therefore, the present invention creatively introduces raspberry ketone glucoside into the formula and finds that it has a certain promoting effect on oil control.

[0030] The seaweed extract obtained by water extraction and alcohol precipitation is rich in seaweed polysaccharides. This polysaccharide active substance has excellent antioxidant, anti-inflammatory, moisturizing effects, and promotes the synthesis of skin collagen and promotes skin barrier repair. At the same time, the seaweed extract also contains mycosporine-like amino acids, which have strong antioxidant and anti-ultraviolet damage effects.

[0031] The Pyracantha fruit extract, obtained through solid-state fermentation with Saccharomyces paradoxus, is rich in flavonoids. Flavonoids can shrink pores, improve uneven skin tone, fight oxidation, scavenge free radicals, slow skin aging, brighten skin tone, promote collagen synthesis, and increase skin elasticity and firmness. It also has anti-inflammatory effects, relieving redness, itching, and other discomforts, making it suitable for sensitive skin. Compared to unfermented Pyracantha fruit extracts or those fermented with other yeasts, the Pyracantha fruit extract provided by the present invention has a higher total flavonoid content, more active substances, and better results.

[0032] The present invention compounds Astragalus membranaceus root extract, raspberry ketone glucoside, Heracleum radix extract, Pyracantha fortuneana fruit extract and bifid yeast fermentation product filtrate to form an efficacy raw material package with complete efficacy and a complete system. The efficacy raw material package can be used alone as an efficacy component after being diluted with a solvent, or can be compounded with auxiliary materials into any dosage form such as essence, emulsion, spray, ointment or cream, etc., and has good adaptability.

[0033] Beneficial effects of the present invention:

[0034] The skin care composition and skin care product provided by the present invention have significant anti-wrinkle, firming and oil-control effects, can effectively shrink pores and improve skin elasticity, and the various ingredients work synergistically to enhance the overall effect. DETAILED DESCRIPTION

[0035] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0036] In order to further illustrate the present invention, the following examples are provided for detailed description. The raw materials used in the following examples and comparative examples of the present invention are all commercially available commodities.

[0037] In the present invention:

[0038] Astragalus membranaceus root extract: Astragalus membranaceus root extract;

[0039] GLOIOPELTIS FURCATA Extract: GLOIOPELTIS FURCATA Extract;

[0040] Pyracantha Fruit Extract: Pyracantha (Pyracantha Fortuneana) Fruit Extract;

[0041] Bifid yeast fermentation product filtrate: purchased from Gansu Yiweisen Pharmaceutical Technology Co., Ltd.

[0042] Saccharomyces paradoxus: scientific name Saccharomyces paradoxus, accession number CGMCC 2.5692, purchased from China General Microbiological Culture Collection Center;

[0043] Saccharomyces cerevisiae (Saccharomyces cerevisiae), accession number CGMCC 2.1527, was purchased from China General Microbiological Culture Collection Center.

[0044] Preparation of Astragalus membranaceus root extract:

[0045] Step 1-1. The Astragalus membranaceus root was dried in a constant temperature drying oven at 50 ° C to a constant weight, and then placed in a grinder and pulverized through a 30-mesh sieve to obtain an Astragalus membranaceus root powder;

[0046] Step 1-2. Astragalus membranaceus root powder and deionized water were mixed and soaked at a mass volume ratio of 1:20 g / mL, and ultrasonically treated at 28 kHz frequency and 55°C for 20-30 min. After ultrasonic treatment, the mixture was kept at 55°C for another 14 h, and passed through a 300 mesh sieve to obtain a crude extract;

[0047] Step 1-3. The crude extract was placed in a vacuum concentrator and concentrated to 1 / 3 of the original volume at 40 kPa and 60° C. to obtain the Astragalus membranaceus root extract.

[0048] Preparation of seaweed extract:

[0049] Step 2-1. Take a certain amount of seaweed and deionized water at a volume mass ratio of 1:30g / mL and mix them. Crush the wall at 25000r / min for 10min to obtain a seaweed homogenate.

[0050] Step 2-2. The seaweed homogenate was heated to 80 ° C and kept warm for 60 minutes. After the end of the incubation, the temperature was reduced to 35 ° C and centrifuged to obtain a supernatant. A 95v / v% ethanol aqueous solution was added to the supernatant and the mixture was soaked for 26 hours. A crystalline precipitate was produced during the soaking process. After the soaking, the precipitate was centrifuged to obtain a precipitate.

[0051] Step 2-3. The precipitate separated in step 2-2 was placed in an oven at 60° C. and dried until there was no alcohol smell, thereby obtaining the seaweed extract.

[0052] Preparation of Pyracantha fortuneana fruit extract:

[0053] Step 3-1. Use a constant temperature drying oven to dry the Pyracantha fruit at 50 ° C to constant weight, place the dried Pyracantha fruit in a grinder and crush through a 60-mesh sieve to obtain Pyracantha fruit powder, moisten the Pyracantha fruit powder with deionized water to obtain a solid fermentation matrix, and sterilize the fermentation matrix in a high-pressure steam cooker at 121 ° C for 10 min and set aside;

[0054] Step 3-2. Take the viable bacteria count as 1×10 8 The bacterial liquid of Saccharomyces cerevisiae with a CFU / mL content was inoculated into the solid fermentation substrate by spraying at a rate of 17% of the mass of the fermentation substrate, and cultured at 28°C, an aeration rate of 0.6 L / min·kg, and a stirring speed of 5 r / min for 72 h to obtain a fermentation material;

[0055] Step 3-3. Mix the fermentation material with an 80 v / v% ethanol aqueous solution at a ratio of 1:15 g / mL, extract under reflux at 75°C for 3 times, each time for 1 h, and combine the extraction filtrates to obtain a filtrate;

[0056] Step 3-4. The filtrate is concentrated under reduced pressure to 1 / 3 of the original volume, and finally vacuum dried to a water content of 8% to obtain the Pyracantha fortuneana fruit extract.

[0057] Total flavonoids yield:

[0058] The extraction filtrate obtained in step 3-3 of the above preparation method is recorded as filtrate ①.

[0059] The extraction filtrate obtained without fermentation treatment is recorded as filtrate ②. The specific preparation method is: first, the Pyracantha fruit is dried at 50°C in a constant temperature drying oven to constant weight, and the dried Pyracantha fruit is placed in a grinder and crushed through a 60-mesh sieve to obtain Pyracantha fruit powder; the Pyracantha fruit powder is mixed with an 80v / v% ethanol aqueous solution at a ratio of 1:15g / mL, and reflux extracted at 75°C for 3 times, each time for 1 hour, and the extraction filtrates are combined to obtain filtrate ②.

[0060] The filtrate obtained by fermentation with Saccharomyces cerevisiae is denoted as filtrate ③. The preparation method is similar to that of filtrate ①, except that Saccharomyces cerevisiae is replaced by Saccharomyces cerevisiae in step 3-2, and the viable cell count is 1×10 8 CFU / mL, and the rest were consistent with filtrate ①.

[0061] The following total flavonoids detection method was used to compare the differences between filtrate ①, filtrate ② and filtrate ③ to verify the influence of different preparation processes on the extraction yield of total flavonoids from Pyracantha fortuneana fruit. The specific detection method is as follows:

[0062] 1Standard curve

[0063] Weigh 0.0125 g of rutin standard, dissolve in ethanol, and dilute to 100 mL to obtain the rutin reference solution. Accurately pipette 0, 2.0, 4.0, 6.0, 8.0, and 10.0 mL of the reference solution into 25 mL volumetric flasks, add 2 mL of 2% aluminum chloride solution and 3 mL of 3% sodium chloride solution, shake well, and incubate at 25°C for 5 minutes. Then, dilute to the mark with 80% ethanol and shake well. Use the 80% ethanol solution as a blank control. Measure the absorbance at 417 nm to create a standard curve.

[0064] 2. Sample solution to be tested

[0065] 1 mL of filtrates ①-③ were respectively measured and placed in 100 mL volumetric flasks, and the volume was adjusted to the mark with 80 v / v% ethanol aqueous solution to obtain sample solutions 1-3 to be tested.

[0066] 3Total flavonoids content

[0067] Accurately measure 2 mL of the sample solution to be tested and place it in a 25 mL volumetric flask. Add 2 mL of 2% aluminum chloride solution and 3 mL of 3% sodium chloride solution. Shake well and incubate at 25°C for 5 minutes to develop color. Then, dilute to the mark with 80% ethanol and shake well. Use 80% ethanol solution as a blank control. Measure the absorbance at a wavelength of 417 nm and calculate the total flavonoid concentration in the sample solution using the regression equation of the standard curve. Calculate the total flavonoid extraction rate using the following formula.

[0068]

[0069] Where:

[0070] C——total flavonoids concentration of the sample solution to be tested, g / L;

[0071] V——total volume of filtrate, L;

[0072] M——total weight of Pyracantha fruit powder, g.

[0073] 4 Test Results

[0074] Table 1 Total flavonoid extraction rate

[0075] Group Total flavonoid extraction rate / % Filtrate ① 4.67 Filtrate② 2.11 Filtrate ③ 3.42

[0076] 5 Results Analysis

[0077] According to the results, the total flavonoids yield in the Pyracantha fruit extract extracted after fermentation with Saccharomyces cerevisiae increased significantly, which was significantly better than the Pyracantha fruit extract extracted only by solvent, and also had more advantages than the extract prepared after fermentation with Saccharomyces cerevisiae. Therefore, Saccharomyces cerevisiae can significantly enrich the active ingredients in Pyracantha fruit, increase the content of active ingredients, and improve the extraction yield of Pyracantha fruit extract.

[0078] Preparation of skin care composition:

[0079] The raw materials were precisely compounded and mixed uniformly according to the mass ratio in Table 1 to obtain a skin care composition.

[0080] Table 1. Raw material mass ratio of skin care composition

[0081] Raw material name Composition 1 Composition 2 Composition 3 Astragalus membranaceus root extract 5 6 8 Raspberry Ketone Glucoside 0.15 0.18 0.2 Seaweed Extract 3 4 5 Pyracantha fruit extract 0.8 0.9 1 Bifidobacterium fermentation filtrate 20 25 30

[0082] Setting up contrast

[0083] In order to verify the functional relationship between the various ingredients in the skin care composition and their contribution to the overall formula, the formula of Composition 2 was modified and performance tests were performed for comparison. The specific control settings are shown in Table 2.

[0084] Table 2 Comparative composition raw material mass ratio

[0085]

[0086] Performance Testing

[0087] 1. Inflammatory factor inhibition test

[0088] 1.1 Test Principle

[0089] The production of TNF-α and IL-6 can lead to excessive oil secretion in the skin. TNF-α can upregulate the expression of sterol regulatory element binding protein 1c (SREBP-1c) mRNA and promote the synthesis of triglycerides in cells. IL-6 can induce the production of adhesion molecule-1 by keratin in the infundibulum of the sebaceous glands of hair follicles, thereby enlarging the sebaceous glands and synthesizing lipids. Therefore, detecting the inhibitory performance of the test substance on inflammatory factors can reflect the oil-control ability of the test substance.

[0090] 1.2 Test substance

[0091] Test substances: Skin care compositions 1-3 and skin care comparison compositions 1-5.

[0092] 1.2 Cytotoxicity assay

[0093] MTT method for cytotoxicity test: After digestion in the logarithmic growth phase, inoculate into a 96-well plate, place the culture plate in a 37°C, 5v / v% CO2 incubator for 18-24h, discard the supernatant, add different amounts of the test substance, so that the mass percentage of the test substance in the whole system is 0.1%, 0.3%, 0.6%, 1%, 2%, 5% and 10%, and continue to culture in a 37°C, 5v / v% CO2 incubator. After 18-24h of cell culture, discard the supernatant, add MTT solution (0.5mg / mL), mix gently, and incubate at 37°C in the dark for 4h. After the incubation is completed, discard the supernatant, add 150μL of DMSO to each well, shake for 20min, and measure the absorbance at 490nm with an enzyme reader. The formula for calculating cell viability is as follows:

[0094]

[0095] The results showed that when the mass percentage of the test substance in the cell culture system was ≤2%, the relative cell survival rate was ≥90%. Therefore, 1% was selected as the safe culture concentration, and the following test was performed.

[0096] 1.3 Detection of inflammatory factor content

[0097] Untreated cells were used as blank controls, cells treated with lipopolysaccharide (LPS) alone were used as negative controls, the positive control was LPS + dexamethasone, and the sample group was LPS + test substance, as shown in the following table:

[0098] Table 3

[0099] Grouping Drug administration Blank control Cell culture medium without any added substances Negative control Cell culture medium containing 0.0005% LPS Positive control Cell culture medium containing 0.0005% LPS and 0.001% dexamethasone Sample Group 1 Cell culture medium containing 0.0005% LPS and 1% Composition 1 Sample Group 2 Cell culture medium containing 0.0005% LPS and 1% Composition 2 Sample Group 3 Cell culture medium containing 0.0005% LPS and 1% Composition 3 Sample Group 4 Cell culture medium containing 0.0005% LPS and 1% comparative composition 1 Sample Group 5 Cell culture medium containing 0.0005% LPS and 1% comparative composition 2 Sample Group 6 Cell culture medium containing 0.0005% LPS and 1% comparative composition 3 Sample Group 7 Cell culture medium containing 0.0005% LPS and 1% comparative composition 4 Sample set 8 Cell culture medium containing 0.0005% LPS and 1% comparative composition 5

[0100] RAW264.7 cells in the logarithmic growth phase were seeded into 24-well plates and incubated at 37°C, 5 v / v% CO2 in an incubator. After 24 hours of culture, the culture medium was aspirated and the cells were gently washed once with PBS. The cells were then grouped and treated according to Table 3. Six replicate wells were set up for each treatment group. The cells were cultured for another 24 hours at 37°C, 5 v / v% CO2, and the supernatant was collected. TNF-α and IL-6 levels were assayed according to the ELISA kit instructions.

[0101] 1.4 Test results

[0102] Table 4 Results of inflammatory factor content

[0103] Group <![CDATA[TNF-α content / pg·mL -1 > <![CDATA[IL-6 content / ng·mL -1 > Blank control 295.1±20.2 1.21±0.15 Negative control 3662.3±125.8 26.18±2.33 Positive control 1008.3±78.3aa 4.83±0.64aa Sample Group 1 1347.8±112.7aa 11.62±1.21aa Sample Group 2 1208.2±73.8aa 10.90±0.75aa Sample Group 3 1366.6±157.6aa 11.23±0.95aa Sample Group 4 2053.7±172.4bb 16.51±1.22bb Sample Group 5 1871.7±122.7bb 17.03±2.16bb Sample Group 6 1922.4±207.1bb 17.35±1.42bb Sample Group 7 2105.8±175.6bb 18.72±2.05bb Sample set 8 1909.1±166.4bb 15.88±1.64bb

[0104] Note: In the table, "a" indicates significant difference compared with the negative control group, P < 0.05, "aa" P < 0.01; "b" indicates significant difference between sample groups 4-8 and sample group 2, P < 0.05, "bb" P < 0.01.

[0105] 1.5 Results Analysis

[0106] According to the test results, compared with the negative control, the inflammatory factor content of sample groups 1-3 was significantly reduced (P < 0.01), indicating that the skin care compositions 1-3 provided by the present invention can effectively inhibit the activity of inflammatory factors, resist the damage of inflammatory factors to the skin, reduce the synthesis of lipids, and achieve the effect of oil control; compared with sample group 2, the inflammatory factor content of sample groups 4-8 was significantly higher than that of sample group 2 (P < 0.01), proving that when any ingredient in the skin care composition is missing, it will affect the effect of the overall formula. Therefore, the Astragalus membranaceus root extract, raspberry ketone glucoside, seaweed extract, Pyracantha fortuneana fruit extract and bifid yeast fermentation product filtrate in the skin care composition are indispensable in the formula, and they have a certain synergistic effect when used in combination.

[0107] 2COL-I expression detection

[0108] 2.1 Test Principle

[0109] COL-I is a major component of the extracellular matrix (ECM) of animals, accounting for 25% to 30% of total protein in the human body. COL-I interacts with the ECM to promote cell growth, adhesion, migration, and deposition; it can also induce chemotaxis of fibroblasts and promote wound repair. COL-I is the most abundant protein in the cell matrix and provides mechanical support to surrounding basal tissue. Reduced COL-I synthesis is a key factor in decreased skin elasticity, enlarged pores, and the formation of wrinkles.

[0110] 2.2 MTT assay for cytotoxicity

[0111] The test method is the same as 1.2.

[0112] 2.3 Detection of COL-I expression

[0113] HFF-1 cells in the logarithmic growth phase were seeded into 24-well plates and incubated at 37°C in a 5 v / v% CO2 incubator. After 24 hours of culture, the culture medium in the plates was aspirated and each plate was gently washed once with PBS. Cell culture medium was added to the blank control group, and cell culture medium containing 1% of the test substance (Compositions 1-3 and Comparative Compositions 1-5) was added to the sample group. Six replicate wells were set up for each treatment group. After 48 hours of culture, the supernatant was collected. COL-I content was detected according to the ELISA kit operating instructions.

[0114] 2.4 Test results

[0115] Table 5 COL-I content results

[0116] Grouping <![CDATA[COL-I content / ng·mL -1 > Blank control 1.61±0.13 Sample Group 1 (containing 1% composition 1) 12.55±1.03aa Sample Group 2 (containing 1% composition 2) 13.08±1.54aa Sample group 3 (containing 1% composition 3) 13.11±0.93aa Sample Group 4 (containing 1% comparative composition 1) 6.33±0.82bb Sample Group 5 (containing 1% comparative composition 2) 7.21±1.14bb Sample Group 6 (containing 1% comparative composition 3) 5.46±0.65bb Sample Group 7 (containing 1% of Comparative Composition 4) 6.37±0.67bb Sample Group 8 (containing 1% of Comparative Composition 5) 8.64±1.31bb

[0117] Note: In the table, "a" indicates significant difference compared with the blank control group, P < 0.05, "aa" P < 0.01; "b" indicates significant difference compared with sample group 2, P < 0.05, "bb" P < 0.01.

[0118] 2.5 Results Analysis

[0119] According to the test results in Table 5, compared with the blank control, the COL-I content of sample groups 1-3 was significantly increased (P < 0.01), indicating that the skin care compositions 1-3 provided by the present invention can effectively promote the synthesis and expression of COL-I in skin cells, improve the firmness of the skin, effectively resist wrinkles and anti-aging, and reduce enlarged pores; compared with sample group 2, the promotion effect of sample groups 4-8 was significantly inferior to that of sample group 2, indicating that when any one of the ingredients in the skin care composition is missing, the firming effect of the overall formula will be affected. Therefore, the Astragalus membranaceus root extract, raspberry ketone glucoside, seaweed extract, Pyracantha fortuneana fruit extract and bifid yeast fermentation product filtrate in the skin care composition are indispensable in the formula, and they have a certain synergistic effect when used in combination.

[0120] 3 Human efficacy tests

[0121] 3.1 Subject selection

[0122] Thirty male subjects with similar skin conditions, aged 30-35 years old, with oily skin and large pores, were selected to undergo pore shrinkage test and skin elasticity test.

[0123] 3.2 Preparation of test samples

[0124] Accurately weigh the raw materials according to the mass percentage in Table 6;

[0125] Step 1. Heat deionized water to 85°C, add sucrose stearate, glyceryl stearate, caprylic / capric triglyceride, and polydimethylsiloxane in sequence, stir evenly at 200 rpm, and keep warm for 20 minutes to obtain a mixed solution A;

[0126] Step 2. Cool the mixed solution A to 50°C, add glycerin, butylene glycol, and phenoxyethanol in sequence, and stir at 200 rpm to obtain a mixture B;

[0127] Step 3. Cool the mixture B to 35° C., add the skin care composition 2, stir evenly at 200 r / min, and add an appropriate amount of pH adjuster to obtain a test sample of the essence.

[0128] Table 6 Raw materials of essence (%)

[0129] Raw material name Essence 1 Essence 2 matrix fluid Skin care composition 2 5 10 / glycerin 3 3 3 Butanediol 2 2 2 Sucrose stearate 1 1 1 Glyceryl Stearate 1 1 1 Caprylic / capric triglyceride 1 1 1 Polydimethylsiloxane 1 1 1 Phenoxyethanol 0.2 0.2 0.2 Deionized water Replenish to 100 Replenish to 100 Replenish to 100

[0130] 3.3 Test plan

[0131] Thirty subjects were randomly divided into three groups, with 10 subjects in each group. They used Essence 1, Essence 2 and Matrix Solution respectively for a comparative test. The subjects applied 2g of Essence / Matrix Solution on their faces, once in the morning and once in the evening every day, for 30 consecutive days.

[0132] Pore detection: Before using the sample and 30 days after use, the skin pore data was measured using the Antera3D Skin Imaging Analyzer. The data before and after use were compared to evaluate the shrinking effect of the test sample on pores, and the data were averaged.

[0133] Skin elasticity testing: Before and 30 days after using the sample, the skin R2 value was measured using a Cutometer MPA580. The closer the R2 is to 1, the better the skin's elasticity. The data before and after use were compared to evaluate the skin firming and anti-wrinkle effects of the test sample, and the average value was taken.

[0134] 3.4 Test Results

[0135] Table 7 Test results

[0136]

[0137]

[0138] 3.5 Results Analysis

[0139] According to the results, compared with the matrix liquid, essences 1 and 2 can effectively tighten pores, improve skin firmness and increase skin elasticity; essence 2 is more effective than essence 1, which proves that the oil-controlling, skin-tightening, firming and anti-wrinkle effects of the essence increase with the increase of the concentration of the skin care composition, showing a certain concentration dependence, indicating that the skin care composition has excellent oil-controlling, pore-shrinking and firming and anti-wrinkle effects.

[0140] In summary, the skin care composition and essence containing the composition provided by the present invention have excellent skin astringent, oil control, anti-wrinkle and anti-aging effects, can effectively shrink pores and improve the effect of enlarged pores. At the same time, they can promote the synthesis of COL-I in skin cells, improve skin firmness, and reduce skin aging.

[0141] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art will be able to modify the technical solutions described in the aforementioned embodiments or substitute equivalents for some of the technical features. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A skin care composition, characterized in that The composition comprises the following components in parts by mass:

2. The skin care composition according to claim 1, characterized in that The preparation method of the Astragalus membranaceus root extract comprises the following steps: Step 1-1. The dried Astragalus membranaceus root was placed in a grinder and pulverized through a 30-mesh sieve to obtain Astragalus membranaceus root powder; Step 1-2. Astragalus membranaceus root powder and deionized water are mixed at a mass volume ratio of 1:20-30 g / mL, and ultrasonically treated at a frequency of 25-30 kHz and 50-60°C for 20-30 min. After ultrasonic treatment, the mixture is kept at 50-60°C for another 12-16 hours, and passed through a 300-400 mesh sieve to obtain a crude extract; Step 1-3. The crude extract is placed in a vacuum concentrator and concentrated to 1 / 3 of the original volume at 30-50 kPa and 60-70° C. to obtain the Astragalus membranaceus root extract.

3. The skin care composition according to claim 1, characterized in that The preparation method of the seaweed extract comprises the following steps: Step 2-1. Take a certain amount of seaweed and deionized water at a volume mass ratio of 1: 20-30g / mL and mix them. Crush the wall at 20000-30000r / min for 10-15min to obtain a seaweed homogenate. Step 2-2. The seaweed homogenate was heated to 70-80 ° C and kept warm for 50-60min. The temperature was lowered to 25-35 ° C and centrifuged to obtain a supernatant. A 95v / v% ethanol aqueous solution was added to the supernatant, mixed and soaked for 24-26h, and centrifuged to obtain a precipitate. Step 2-3. The precipitate obtained by separation in step 2-2 is placed in an oven at 50-60° C. and dried until there is no alcohol smell, thereby obtaining the seaweed extract.

4. The skin care composition according to claim 1, characterized in that The preparation method of the Pyracantha fortuneana fruit extract comprises the following steps: Step 3-1. Take a certain amount of dried Pyracantha fruit and place it in a grinder and grind it through a 60-mesh sieve to obtain Pyracantha fruit powder. Moisten the Pyracantha fruit powder with deionized water to obtain a water content of 40-50% to obtain a solid fermentation matrix, which is sterilized for standby use. Step 3-2: Take the viable cell count ≥ 1×10 8 The bacterial liquid of Saccharomyces cerevisiae with a CFU / mL is inoculated into the solid fermentation substrate by spraying at 15-20% of the mass of the fermentation substrate, and cultured at 28-30°C, a ventilation rate of 0.5-0.7 L / min·kg, and a stirring speed of 3-7 r / min for 72-80 hours to obtain a fermentation material; Step 3-3. Mix the fermentation material with a 70-80 v / v% ethanol aqueous solution at a ratio of 1:15-20 g / mL, extract under reflux at 70-80°C for 3 times, each time for 1-1.2 hours, combine the extraction filtrates, concentrate the filtrates under reduced pressure to 1 / 3 of the original volume, and finally vacuum dry them to a water content of ≤10% to obtain a Pyracantha fruit extract.

5. Use of the skin care composition according to any one of claims 1 to 4 in preparing skin care products with anti-wrinkle, firming and oil-control effects.

6. An essence with the effects of shrinking pores, rejuvenating the skin and anti-wrinkle, characterized in that: The essence contains the skin care composition according to any one of claims 1 to 4.

7. The essence according to claim 6, characterized in that The amount of the skin care composition added to the essence is 5-10 wt %.

8. The essence according to claim 7, characterized in that The essence also contains preservatives, antioxidants, moisturizers and solvents.

9. The method for preparing the essence according to claim 8, characterized in that: The preparation method comprises the following steps: The skin care composition, preservative, antioxidant, moisturizer and solvent are mixed and stirred evenly to obtain the essence.

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