Natural oil control composition containing plant extract and microbial fermentation product and application of natural oil control composition

Abstract

The invention discloses a natural oil control composition containing a plant extract and a microbial fermentation product and application of the natural oil control composition, and belongs to the technical field of daily skin care products. The composition is prepared from the following raw materials: a saw palmetto fruit extract, a pseudoalteromonas marina fermentation product, 0.5 to 0.75 percent of a pomegranate fruit extract, glycerol, butanediol, carbomer, triethanolamine and deionized water. The composition disclosed by the invention not only can effectively control oil, but also can keep moisture balance of skin and avoid dry skin caused by oil control. After the product is used, the skin feels fresh and cool and does not feel tight, so that the product has a good moisturizing effect. And the composition is safe, free of chemical synthesis components, low in irritation, capable of comprehensively improving the grease balance of skin and the overall health condition, and suitable for long-term use.

Description

Technical Field

[0001] The present invention belongs to the technical field of daily skin care products, and particularly relates to a natural oil-control composition comprising plant extracts and microbial fermentation products and applications thereof. Background Art

[0002] As people pay more and more attention to skin health and beauty, the research and development of oil-control cosmetics has also made significant progress. Existing oil-control technologies are mainly divided into two categories: chemical oil control and natural ingredient oil control.

[0003] Chemical oil-control ingredients, such as salicylic acid and benzoic acid, can effectively inhibit sebum secretion, but long-term use can cause skin irritation and allergies, and their effectiveness gradually diminishes due to tolerance. Furthermore, these chemical ingredients can disrupt the skin's microecological balance and cause other skin problems.

[0004] Natural ingredients such as tea tree oil and calendula extract are widely used for oil control. However, the effectiveness of a single natural ingredient is limited and cannot meet diverse skin needs. For example, while tea tree oil has antibacterial and oil-control properties, its high volatility can cause skin drying. Witch hazel extract, while it can tighten pores, has a weak direct inhibitory effect on sebum secretion.

[0005] For example, Chinese patent application CN 106661676 A discloses a plant extract for controlling oil and shrinking pores, as well as its preparation method and application. The raw material components include: 15-25 parts by weight of rice fermentation liquid, 35-35 parts by weight of calendula extract, 15-25 parts by weight of aloe vera extract, and 25-35 parts by weight of palmetto oil.

[0006] However, current oil-control products offer limited functionality and ineffective oil-control, resulting in limited improvement in the "shine" effect on oily skin and failing to meet consumers' demand for a refreshed, healthy-looking skin. Therefore, there is an urgent need to develop a multifunctional, highly effective oil-control composition to meet current consumer needs. Summary of the Invention

[0007] In response to the problems existing in the prior art, the present invention provides a natural oil-control composition comprising plant extracts and microbial fermentation products. When used as an emulsion or essence, it can effectively control oil, soothe, inhibit bacteria and moisturize the skin, and comprehensively improve the skin's oil balance and overall health. It is particularly suitable for oily skin and combination skin.

[0008] In order to achieve the above technical objectives, the technical solution adopted by the present invention is: A natural oil-control composition comprising plant extracts and microbial fermentation products, comprising the following raw materials, in percentage by mass: 0.5-0.75% saw palmetto fruit extract, 0.5-0.75% Pseudoalteromonas marine fermentation product, 0.5-0.75% pomegranate fruit extract, 1-3% glycerol, 1-3% butylene glycol, 3-5% carbomer, 1-3% triethanolamine, and the balance deionized water, with the total amount being 100%.

[0009] Furthermore, the preparation method of the saw palmetto fruit extract is: A. Grind the dried saw palmetto berries into 40-60 meshes and remove impurities to obtain berry powder; B. The fruit powder was mixed with 70-80% ethanol at a solid-liquid ratio of 1:10, and the mixture was soaked at room temperature for 12 hours. The mixture was then ultrasonically treated at a power of 300 W, a frequency of 40 kHz, a temperature of 50°C, and a time of 30 minutes. The mixture was then centrifuged at 4000 rpm for 10 minutes, and the supernatant was concentrated under reduced pressure, sterilized, and freeze-dried to obtain a saw palmetto fruit extract.

[0010] Furthermore, the preparation method of the Pseudoalteromonas maritima fermentation product is: A. Inoculate the Pseudoalteromonas marineus strain CGMCC No. 1.14991 into the seed culture medium at a 1% V / V inoculation rate and culture at 18-20°C and 180 rpm for 24 hours until the OD 600 is 2.0-3.0, and seed solution is obtained; B. The seed liquid was inoculated into the fermentation medium at an inoculum rate of 5% V / V, and the fermentation culture was carried out at 18-20° C. for 72 hours. The pH value was controlled at 7.0-7.3 and the dissolved oxygen content was controlled at 30-50%. When the fermentation was carried out for 24 hours, FeCl3 was added at a concentration of 0.05% by mass of the fermentation medium. After the fermentation was completed for 72 hours, the fermentation liquid was heated to 70-80° C. for 30 minutes to inactivate the bacteria, and high-pressure homogenization was performed at 1500 bar for 3 times to obtain a treated liquid. C. The treated liquid was then centrifuged at 8000 rpm for 15 minutes at 4°C to collect the supernatant; the supernatant was concentrated to 1 / 5 of the original volume at 40°C and -0.08 MPa, and then 1% trehalose and 0.5% EDTA-2Na were added based on the mass of the supernatant. The mixture was stirred and then sterilized and freeze-dried to obtain the fermentation product of Pseudoalteromonas marinus.

[0011] Furthermore, the seed culture medium composition is: peptone 5.0 g, yeast extract 1.0 g, glucose 10.0 g, MgSO4·7H2O: 0.5 g, KH2PO4 0.2 g, deionized water to 1 L, pH 7.0-7.3, sterilized before use; the fermentation medium composition is: glycerol: 20 mL / L, L-glutamic acid: 1.0 g / L added to the seed culture medium.

[0012] Furthermore, the Pseudoalteromonas haloplanktis was purchased from the China General Microorganism Collection Center, and the original preservation date was November 28, 2014. The strain can be purchased through commercial channels without the need for biological preservation.

[0013] Furthermore, the preparation method of the pomegranate fruit extract is as follows: the pomegranate peel is dried to a moisture content of ≤8%, crushed through a 40-mesh sieve to obtain pomegranate fruit powder, the pomegranate fruit powder is mixed with 50% ethanol by mass concentration at a material-liquid ratio of 1:15, and ultrasonically treated with an ultrasonic power of 400 W, a frequency of 40 kHz, a temperature of 50°C, and a time of 20 minutes; after the treatment is completed, centrifugation is performed at 4000 rpm for 10 minutes, the supernatant is filtered through a 0.45 μm filter membrane, the filtrate is sterilized, and freeze-dried to obtain the pomegranate fruit extract.

[0014] A method for preparing a natural oil-control composition comprising a plant extract and a microbial fermentation product comprises the following steps: A. Accurately weigh the raw materials of each component of the preparation formula and set aside; B. Preparation of aqueous phase: Add water to an emulsifier under stirring conditions, and then add glycerin, butylene glycol, and carbomer in that order. Turn on the stirring device and stir at 500-1000 rpm until completely dissolved, ensuring uniform mixing and no residual particles. C. Adjust pH: Slowly add triethanolamine while stirring continuously to adjust the pH of the mixture to between 6.0 and 7.0; D. When the temperature of the mixed liquid drops below 40°C, slowly add saw palmetto fruit extract, Pseudoalteromonas marinus fermentation product, and pomegranate fruit extract into the mixed liquid. Stir at 200-500 rpm for 30 minutes to ensure that the active ingredients are evenly dispersed in the mixed liquid. Finally, sterilize and fill the mixture.

[0015] The invention discloses an application of a natural oil-control composition comprising plant extracts and microbial fermentation products, which can be used as an essence or lotion after cleansing the face.

[0016] Beneficial effects (1) The present invention screens natural plant extracts and microbial fermentation products that can regulate oil secretion as active ingredients. Among them, saw palmetto fruit extract can inhibit the activity of 5α-reductase and reduce the excessive secretion of sebaceous glands from the source. At the same time, saw palmetto fruit extract contains natural antibacterial ingredients that can inhibit the growth of harmful bacteria such as Propionibacterium acnes and reduce the risk of skin infection. The fermentation product of Pseudomonas aeruginosa has good moisturizing properties, which can enhance the barrier function of the skin, reduce water loss, and keep the skin moisturized. On the other hand, the strain metabolites can inhibit lipid accumulation. At the same time, the bioactive enzymes such as lipase and esterase contained therein can specifically hydrolyze triglycerides into free fatty acids and glycerol, directly reducing sebum accumulation and controlling oil and anti-seborrheic mechanisms, further enhancing the oil control effect. The added pomegranate fruit extract is rich in ellagic acid and polyphenols, which can quickly converge the dilated hair follicle opening and reduce the pore blockage caused by oil oxidation. After the three are mixed in equal proportions, they synergistically inhibit oil secretion, forming a complete oil control chain of "inhibition-decomposition-convergence" to achieve efficient oil control.

[0017] (2) The composition of the present invention not only effectively controls oil but also maintains the skin's moisture balance, preventing dryness caused by oil control. After using the product of the present invention, the skin feels refreshed and free of tightness, indicating that the product has a good moisturizing effect. Furthermore, the composition is safe, contains no chemically synthesized ingredients, and is low in irritation. It can comprehensively improve the skin's oil balance and overall health, making it suitable for long-term use. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 The effects of different mass concentrations of saw palmetto fruit extracts on sebaceous gland cell activity; Figure 2 The effects of different mass concentrations of Pseudoalteromonas marine fermentation products on sebaceous gland cell activity; Figure 3 The effects of different mass concentrations of pomelo extracts on sebaceous gland cell activity; Figure 4 These are pictures of lipid droplet detection results in the embodiment and model groups; Figure 5 These are pictures taken by the VISIA facial image analyzer before and after using the product of Example 1, where af is before use and mr is after use. DETAILED DESCRIPTION

[0019] The technical solution of the present invention will be further described below with reference to specific embodiments, but is not limited thereto.

[0020] Example 1 A natural oil-control composition comprising plant extracts and microbial fermentation products, comprising the following raw materials, in percentage by mass: 0.5% saw palmetto fruit extract, 0.5% Pseudoalteromonas marinus fermentation product, 0.5% pomegranate fruit extract, 1% glycerin, 3% butylene glycol, 3% carbomer, 3% triethanolamine, and the balance deionized water, with a total amount of 100%.

[0021] The preparation method of the saw palmetto fruit extract is: A. Grind the dried saw palmetto berries into 40-60 meshes and remove impurities to obtain berry powder; B. The fruit powder was mixed with 70-80% ethanol at a solid-liquid ratio of 1:10, and the mixture was soaked at room temperature for 12 hours. The mixture was then ultrasonically treated at a power of 300 W, a frequency of 40 kHz, a temperature of 50°C, and a time of 30 minutes. The mixture was then centrifuged at 4000 rpm for 10 minutes, and the supernatant was concentrated under reduced pressure, sterilized, and freeze-dried to obtain a saw palmetto fruit extract.

[0022] The preparation method of the Pseudoalteromonas maritima fermentation product is as follows: A. Inoculate the Pseudoalteromonas marineus strain CGMCC No. 1.14991 into the seed culture medium at a 1% V / V inoculation rate and culture at 18-20°C and 180 rpm for 24 hours until the OD 600 is 2.0-3.0, and seed solution is obtained; B. The seed liquid was inoculated into the fermentation medium at an inoculum rate of 5% V / V, and the fermentation culture was carried out at 18-20° C. for 72 hours. The pH value was controlled at 7.0-7.3 and the dissolved oxygen content was controlled at 30-50%. When the fermentation was carried out for 24 hours, FeCl3 was added at a concentration of 0.05% by mass of the fermentation medium. After the fermentation was completed for 72 hours, the fermentation liquid was heated to 70-80° C. for 30 minutes to inactivate the bacteria, and high-pressure homogenization was performed at 1500 bar for 3 times to obtain a treated liquid. C. The treated liquid was then centrifuged at 8000 rpm for 15 minutes at 4°C to collect the supernatant; the supernatant was concentrated to 1 / 5 of the original volume at 40°C and -0.08 MPa, and then 1% trehalose and 0.5% EDTA-2Na were added based on the mass of the supernatant. The mixture was stirred and then sterilized and freeze-dried to obtain the fermentation product of Pseudoalteromonas marinus.

[0023] The seed culture medium composition is: peptone 5.0 g, yeast extract 1.0 g, glucose 10.0 g, MgSO4·7H2O: 0.5 g, KH2PO4 0.2 g, deionized water to 1 L, pH 7.0-7.3, sterilized before use; the fermentation medium composition is: glycerol: 20 mL / L, L-glutamic acid: 1.0 g / L added to the seed culture medium.

[0024] The Pseudoalteromonas haloplanktis was purchased from the China General Microorganism Collection Center, with the original deposit date being November 28, 2014. The strain can be purchased through commercial channels without the need for biological preservation.

[0025] The preparation method of the pomegranate fruit extract comprises the following steps: drying pomegranate peel to a moisture content of ≤8%, crushing the peel through a 40-mesh sieve to obtain pomegranate fruit powder, mixing the pomegranate fruit powder with 50% ethanol at a material-liquid ratio of 1:15, and ultrasonically treating the peel at an ultrasonic power of 400 W, a frequency of 40 kHz, a temperature of 50° C., and a time of 20 minutes; after the treatment, centrifuging the peel at 4000 rpm for 10 minutes, filtering the supernatant through a 0.45 μm filter membrane, sterilizing the filtrate, and freeze-drying the resulting pomegranate fruit extract.

[0026] A method for preparing a natural oil-control composition comprising a plant extract and a microbial fermentation product comprises the following steps: A. Accurately weigh the raw materials of each component of the preparation formula and set aside; B. Preparation of aqueous phase: Add water to an emulsifier under stirring conditions, and then add glycerin, butylene glycol, and carbomer in that order. Turn on the stirring device and stir at 500-1000 rpm until completely dissolved, ensuring uniform mixing and no residual particles. C. Adjust pH: Slowly add triethanolamine while stirring continuously to adjust the pH of the mixture to between 6.0 and 7.0; D. When the temperature of the mixed liquid drops below 40°C, slowly add saw palmetto fruit extract, Pseudoalteromonas marinus fermentation product, and pomegranate fruit extract into the mixed liquid. Stir at 200-500 rpm for 30 minutes to ensure that the active ingredients are evenly dispersed in the mixed liquid. Finally, sterilize and fill the mixture.

[0027] The invention discloses an application of a natural oil-control composition comprising plant extracts and microbial fermentation products, which can be used as an essence or lotion after cleansing the face.

[0028] Example 2 A natural oil-control composition comprising plant extracts and microbial fermentation products, comprising the following raw materials, in percentage by mass: 0.75% saw palmetto fruit extract, 0.75% Pseudoalteromonas marine fermentation product, 0.75% pomegranate fruit extract, 3% glycerol, 1% butylene glycol, 3% carbomer, 1% triethanolamine, and the balance deionized water, with a total amount of 100%.

[0029] The preparation method of the saw palmetto fruit extract is: A. Grind the dried saw palmetto berries into 40-60 meshes and remove impurities to obtain berry powder; B. The fruit powder was mixed with 70-80% ethanol at a solid-liquid ratio of 1:10, and the mixture was soaked at room temperature for 12 hours. The mixture was then ultrasonically treated at a power of 300 W, a frequency of 40 kHz, a temperature of 50°C, and a time of 30 minutes. The mixture was then centrifuged at 4000 rpm for 10 minutes, and the supernatant was concentrated under reduced pressure, sterilized, and freeze-dried to obtain a saw palmetto fruit extract.

[0030] The preparation method of the Pseudoalteromonas marinus fermentation product is the same as that in Example 1.

[0031] The Pseudoalteromonas haloplanktis strain, CGMCC No. 1.14991, was purchased from the China General Microorganism Collection Center with an original deposit date of November 28, 2014. This strain can be purchased through commercial channels without the need for biological deposit. Same as Example 1.

[0032] The pomegranate fruit extract is prepared by drying pomegranate peel to a moisture content of ≤8%, crushing the peel through a 40-mesh sieve to obtain pomegranate fruit powder, mixing the pomegranate fruit powder with 50% ethanol at a material-to-liquid ratio of 1:15, and ultrasonically treating the mixture at a power of 400 W, a frequency of 40 kHz, a temperature of 50° C., and a time of 20 minutes. After the treatment, the mixture is centrifuged at 4000 rpm for 10 minutes, the supernatant is filtered through a 0.45 μm filter membrane, and the filtrate is sterilized and freeze-dried to obtain the pomegranate fruit extract. The same method as in Example 1 is used. A method for preparing a natural oil-control composition comprising a plant extract and a microbial fermentation product comprises the following steps: A. Accurately weigh the raw materials of each component of the preparation formula and set aside; B. Preparation of aqueous phase: Add water to an emulsifier under stirring conditions, and then add glycerin, butylene glycol, and carbomer in that order. Turn on the stirring device and stir at 500-1000 rpm until completely dissolved, ensuring uniform mixing and no residual particles. C. Adjust pH: Slowly add triethanolamine while stirring continuously to adjust the pH of the mixture to between 6.0 and 7.0; D. When the temperature of the mixed liquid drops below 40°C, slowly add saw palmetto fruit extract, Pseudoalteromonas marinus fermentation product, and pomegranate fruit extract into the mixed liquid. Stir at 200-500 rpm for 30 minutes to ensure that the active ingredients are evenly dispersed in the mixed liquid. Finally, sterilize and fill the mixture.

[0033] The invention discloses an application of a natural oil-control composition comprising plant extracts and microbial fermentation products, which can be used as an essence or lotion after cleansing the face.

[0034] Comparative Examples 1-6 Compared with Example 1, comparative experiments were conducted by varying the core functional ingredients, namely, Comparative Examples 1-3 using only the single ingredients of saw palmetto fruit extract, Pseudoalteromonas marinus fermentation product, and pomegranate fruit extract, and Comparative Examples 4-6 using different dosage ratios to verify the relationship between the core functional ingredients. The comparative example formulas are as follows: Table 1 Composition ratio (mass percentage, total amount is 100%) Cytotoxicity assay: Sebaceous gland cell culture: Sebaceous gland cells frozen in liquid nitrogen were quickly revived and cultured with 10% fetal bovine serum (FBS) and 1×10 5 Culture the cells in DMEM high-glucose medium supplemented with 100 U / L penicillin and 100 mg / L streptomycin at 37°C and 5% carbon dioxide. When the cells reached a confluence exceeding 90% adherent growth, they were digested and passaged using trypsin containing 0.25% disodium ethylenediaminetetraacetic acid (EDTA). Cells in the logarithmic growth phase were used for subsequent experiments.

[0035] CCK-8 assay to detect cell viability: Cells were plated at 1×10 4 Cells were inoculated into 96-well plates and cultured overnight before treatment. Multiple replicate wells were set up. Different concentrations (0.1-5%) of saw palmetto fruit extract, Pseudoalteromonas marinus fermentation product, and pomegranate fruit extract were added to the replicate wells of the experimental group. The wells without cells served as blank groups. After treatment for the corresponding time, CCK-8 solution was added and incubated at 37°C for 2 hours. The absorbance at 450 nm was measured. Each treatment group was repeated three times, and the results were averaged (refer to CHEN KC, YANG CH, LI TT, et al. Suppression of Propionibacterium acnes- stimulated proinflammatory cytokines by Chinese bayberry extracts and its active constituent myricetin in human sebocytes in vitro [J]. Phytotherapy research, 2019, 33(4): 1104-1113).

[0036] The experimental results are as follows Figure 1-3As shown, saw palmetto fruit extract, Pseudoalteromonas marinus fermentation product, and durian fruit extract did not affect cell viability at concentrations between 0.1 and 5%, and all exhibited some promoting activity, indicating that these three ingredients are relatively safe. Furthermore, the effects were significant and stable within the 0.1-0.8% range, so the present invention selected an active ingredient concentration of 0.5-0.75% as the optimal concentration.

[0037] Safety evaluation for use: Human skin patch test: The compositions prepared in Examples 1-2 and Comparative Examples 1-6 were subjected to the human skin patch test in accordance with the "2022 Cosmetic Safety Technical Specifications".

[0038] Evaluation criteria: Grade 0: negative reaction; Grade 1: Suspicious reaction, only slight erythema; Grade 2: weak positive reaction, erythema, infiltration, edema, and papules may be present; Grade 3: Strong positive reaction, with erythema, infiltration, edema, papules, and the reaction may extend beyond the test area; Grade 4: Very strong positive reaction, obvious erythema, severe infiltration, edema, confluent blister, and reaction beyond the test area.

[0039] Table 2 Test results The human skin patch test proved that the composition is relatively safe.

[0040] Nile red staining was used to detect the effect of samples on lipid secretion in SZ95 cells: SZ95 cells grown to the logarithmic phase were cultured at 3×10 5 Cells were seeded at a density of 100 μg / mL in a 24-well plate, with 1 mL of cell suspension per well. The cells were incubated in a 37°C, 5% CO2 incubator for 24 hours. When the cells reached near confluency, the Example and Comparative Example samples were administered, with triplicate wells per group. In the experiment, 6 μg / mL testosterone and 100 μmol / L linoleic acid were used to induce lipid synthesis. After 24 hours of incubation, the culture medium was discarded, the cells were washed at least twice with PBS, and fixed with 4% paraformaldehyde at room temperature for 10 minutes. After fixation, the cells were stained with 100 μg / mL Nile Red dye and incubated in a 37°C incubator for an additional 10 minutes. The dye was aspirated, and the cells were then washed three times with PBS. The cell slides were gently removed and placed on a glass slide. Staining was observed under a fluorescence microscope. Cells with strong orange-red fluorescence were positive for lipid-rich cells. Fluorescence intensity was analyzed using ImageJ software.

[0041] Table 3 Effects of different sample groups on lipid secretion in SZ95 cells Note: Compared with the model control, P<0.05 indicates a significant difference; P<0.01 indicates an extremely significant difference.

[0042] From the data in Table 3, it can be seen that the inhibition rate of lipid in SZ95 cells by the samples of the embodiment group of the present invention is above 45%, and there is a significant difference between them and the model group (P < 0.05). However, in the comparative examples 1-6, where the active ingredients are changed, the synergistic balance between the three is broken, and the synergistic inhibition effect on lipid is reduced, thus the inhibitory effect is reduced. Figure 4 It can be seen intuitively from the cell staining pictures that the neutral lipid secretion of SZ95 sebaceous gland cells in the experimental group cells to which the sample of the present invention is added is significantly reduced.

[0043] Efficacy experiment: Inclusion criteria: Ages 20-35, acne vulgaris, no history of cosmetic allergies, and no serious systemic diseases. Exclusion criteria: Current participation in other clinical trials; Use of oral or topical cosmetic products (corticosteroids, antibiotics, tretinoin, etc.) within 2 weeks that could affect the results of the trial; Unresolved skin diseases; Previous laser cosmetic or chemical peels on the test area.

[0044] The final number of participants included in the test was 30. This study strictly adhered to the Declaration of Helsinki, and all subjects understood the purpose and process of the experiment and signed informed consent.

[0045] Testing Method: The test lasted five days. After cleansing their faces morning and night, volunteers in the experimental group applied an appropriate amount of the present invention's examples or comparative example samples (experimental group). The control group applied an appropriate amount of the control group sample (daily used essence product) and massaged until absorbed. This was done once daily, morning and night. Volunteers were not allowed to use other skincare products during the test period.

[0046] Test indicators: Oil secretion: Indicates the sebum content of the face. The smaller the value, the less oil the skin secretes.

[0047] The percentage of red area (also known as proportion): represents the redness value of the skin. A decrease in the proportion of red area means good anti-inflammatory and soothing effects, and the ability to repair acne scars.

[0048] Facial porphyrin value: The facial images of the subjects were taken using VISIA, and the facial porphyrin value was analyzed using Image Pro Plus software. The facial porphyrin value represents the metabolic activity of facial microorganisms.

[0049] Facial imaging: Use VISIA to capture the subject's facial image and compare the pictures before and after the test.

[0050] Table 4 Human efficacy test results Changes in sebum production on the skin surface can reflect the product's oil-control effectiveness during the testing period. The experimental group showed significantly lower sebum secretion compared to the control group. A greater proportion of red areas on the skin indicates more severe acne and more sensitive skin. The proportion of red areas on the skin was lower in the experimental group compared to the control group. Porphyrins are substances produced by skin microbial metabolism that fluoresce under ultraviolet light and are often used to monitor the growth and reproduction of facial microorganisms. Porphyrin levels were lower in the experimental group compared to the control group. Figure 5 The results of the human efficacy evaluation show that the facial acne and red areas of volunteers before and after use of Example 1 are as follows. It can be seen that the proportion of acne and red areas of volunteers (experimental group) gradually decreased, while the acne of volunteers (control group) showed no improvement. The above human efficacy evaluation results show that the product of the embodiment of the present invention has certain oil control, soothing and antibacterial effects. It should be noted that the above embodiments are only some of the preferred embodiments of the present invention, and not all of them. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work should fall within the scope of protection of the present invention.

Claims

1. A natural oil control composition comprising plant extracts and microbial fermentation products, characterized in that: The preparation comprises the following raw materials in percentage by mass: 0.5-0.75% saw palmetto fruit extract, 0.5-0.75% Pseudoalteromonas sea fermentation product, 0.5-0.75% pomegranate fruit extract, 1-3% glycerol, 1-3% butylene glycol, 3-5% carbomer, 1-3% triethanolamine, and the balance deionized water, with a total amount of 100%.

2. The natural oil control composition comprising plant extracts and microbial fermentation products according to claim 1, characterized in that: The preparation method of the saw palmetto fruit extract is: A. Grind the dried saw palmetto berries into 40-60 meshes and remove impurities to obtain berry powder; B. The fruit powder was mixed with 70-80% ethanol at a solid-liquid ratio of 1:10, and the mixture was soaked at room temperature for 12 hours. The mixture was then ultrasonically treated at a power of 300 W, a frequency of 40 kHz, a temperature of 50°C, and a time of 30 minutes. The mixture was then centrifuged at 4000 rpm for 10 minutes, and the supernatant was concentrated under reduced pressure, sterilized, and freeze-dried to obtain a saw palmetto fruit extract.

3. The natural oil control composition comprising plant extracts and microbial fermentation products according to claim 1, characterized in that: The preparation method of the Pseudoalteromonas maritima fermentation product is as follows: A. Inoculate the Pseudoalteromonas marineus strain CGMCC No. 1.14991 into the seed culture medium at a 1% V / V inoculation rate and culture at 18-20°C and 180 rpm for 24 hours until the OD 600 is 2.0-3.0, and seed solution is obtained; B. The seed liquid was inoculated into the fermentation medium at an inoculum rate of 5% V / V, and the fermentation culture was carried out at 18-20° C. for 72 hours. The pH value was controlled at 7.0-7.3 and the dissolved oxygen content was controlled at 30-50%. When the fermentation was carried out for 24 hours, FeCl3 was added at a concentration of 0.05% by mass of the fermentation medium. After the fermentation was completed for 72 hours, the fermentation liquid was heated to 70-80° C. for 30 minutes to inactivate the bacteria, and high-pressure homogenization was performed at 1500 bar for 3 times to obtain a treated liquid. C. The treated liquid was then centrifuged at 8000 rpm for 15 minutes at 4°C, and the supernatant was collected. The supernatant was concentrated to 1 / 5 of the original volume at 40°C and -0.08 MPa, and 1% trehalose and 0.5% EDTA-2Na based on the mass of the supernatant were added. The mixture was stirred and then sterilized and freeze-dried to obtain the fermentation product of Pseudomonas maritima.

4. The natural oil control composition comprising plant extracts and microbial fermentation products according to claim 3, characterized in that: The seed culture medium composition is: peptone 5.0 g, yeast extract 1.0 g, glucose 10.0 g, MgSO4·7H2O 0.5 g, KH2PO4 0.2 g, and deionized water to 1 L, pH 7.0-7.3, and used after sterilization; the fermentation medium composition is: glycerol: 20 mL / L, L-glutamic acid: 1.0 g / L added to the seed culture medium.

5. The natural oil control composition comprising plant extracts and microbial fermentation products according to claim 1, characterized in that: The preparation method of the pomegranate fruit extract comprises the following steps: drying pomegranate peel to a moisture content of ≤8%, crushing the peel through a 40-mesh sieve to obtain pomegranate fruit powder, mixing the pomegranate fruit powder with 50% ethanol by mass at a material-liquid ratio of 1:15, and ultrasonically treating the peel at an ultrasonic power of 400 W, a frequency of 40 kHz, a temperature of 50° C., and a time of 20 minutes; after the treatment, centrifuging the peel at 4000 rpm for 10 minutes, filtering the supernatant through a 0.45 μm filter membrane, sterilizing the filtrate, and freeze-drying the resulting pomegranate fruit extract.

6. A method for preparing the natural oil-control composition comprising a plant extract and a microbial fermentation product according to any one of claims 1 to 5, characterized in that: The method comprises the following preparation steps: A. Accurately weigh the raw materials of each component of the preparation formula and set aside; B. Preparation of aqueous phase: Add water to an emulsifier under stirring conditions, and then add glycerin, butylene glycol, and carbomer in that order. Turn on the stirring device and stir at 500-1000 rpm until completely dissolved, ensuring uniform mixing and no residual particles. C. Adjust pH: Slowly add triethanolamine while stirring continuously to adjust the pH of the mixture to between 6.0 and 7.0; D. When the temperature of the mixed liquid drops below 40°C, slowly add saw palmetto fruit extract, Pseudoalteromonas marinus fermentation product, and pomegranate fruit extract into the mixed liquid. Stir at 200-500 rpm for 30 minutes to ensure that the active ingredients are evenly dispersed in the mixed liquid. Finally, sterilize and fill the mixture.

7. Use of the natural oil control composition comprising a plant extract and a microbial fermentation product according to any one of claims 1 to 5, characterized in that: After cleansing, use as a serum or lotion.

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