Vaseline-containing skin care product with itching relieving and chilblain removing effects and preparation method thereof

Abstract

The invention discloses a Vaseline-containing skin care product with the effects of relieving itching and removing chilblain and a preparation method of the Vaseline-containing skin care product, and relates to the technical field of skin care products. Comprising 35%-55% of vaseline, 3%-8% of a bacillus fermentation product, 1%-3% of a mint leaf extract, 2%-5% of a Chinese ilex leaf extract, 0.5%-1.5% of rose essential oil, 4%-10% of an emulsifier, 0.5%-2.5% of a transdermal enhancer and a proper amount of water. The traditional Chinese medicine composition can inhibit the release of histamine, alleviate the inflammatory response of chilblain parts, and improve the prevention and treatment effect of chilblain; moreover, the components are combined together to achieve the effects of diminishing inflammation, removing necrotic tissue and promoting granulation, so that a relatively long storage life can be achieved even if a preservative is not added.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care products, and more particularly to a skin care product containing vaseline and having the effects of relieving itching and removing frostbite, and a preparation method thereof. Background Art

[0002] Chilblains are a common winter skin condition caused primarily by localized blood circulation impairment due to the cold. Patients often experience symptoms such as redness, itching, and pain, and in severe cases, blisters and ulcers may develop. Currently, chilblain treatments on the market often contain irritating ingredients such as camphor and menthol, which can cause skin allergic reactions with long-term use.

[0003] As a classic skin moisturizer, Vaseline has good sealing and moisturizing effects, but the treatment effect of Vaseline alone on frostbite is limited.

[0004] Therefore, it is of great significance to develop a frostbite treatment product that combines the basic properties of vaseline with highly effective antipruritic ingredients and has good permeability. Summary of the Invention

[0005] In view of this, the present invention provides a skin care product containing vaseline and having the effects of relieving itching and removing frostbite, and a preparation method thereof, which overcomes the above-mentioned defects that the treatment effect of using vaseline alone on frostbite is limited, and that most frostbite treatment products on the market contain irritating ingredients such as camphor and menthol.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] In one aspect, the present invention provides a skin care product containing vaseline and having the effects of relieving itching and frostbite. The skin care product comprises the following ingredients, calculated by mass percentage:

[0008] Vaseline 35%-55%, Bacillus fermentation product 3%-8%, peppermint leaf extract 1%-3%, holly leaf extract 2%-5%, rose essential oil 0.5%-1.5%, emulsifier 4%-10%, transdermal enhancer 0.5%-2.5% and appropriate amount of water.

[0009] Compared to existing technologies, the present invention utilizes petrolatum, Bacillus fermentation products, peppermint leaf extract, wintergreen leaf extract, and rose essential oil as the primary ingredients of an anti-itch and anti-frostbite skincare product. Vaseline, as the primary oil component in the formula, possesses strong occlusive properties, forming a physical barrier on the skin surface, reducing moisture evaporation and alleviating the dryness and cracking of the skin caused by frostbite. Bacillus fermentation products are rich in active substances such as amino acids, vitamins, and peptides, which can enhance skin immunity, reduce skin inflammation, and reduce nerve ending sensitivity, thereby alleviating the intense itching associated with frostbite. Peppermint leaf extract stimulates the skin's cold receptors, producing a cooling sensation that masks itch signals, achieving sensory relief. It also inhibits histamine release, alleviating inflammatory reactions (such as redness, swelling, and burning) at the frostbite site. Combined with the occlusive properties of petrolatum, it enhances the soothing effect. Wintergreen leaf extract contains salicylic acid and flavonoids, which can inhibit common pathogens such as Staphylococcus aureus, reducing the risk of infection in frostbite wounds. Salicylic acid provides mild exfoliation, helping to remove necrotic tissue in the frostbite area and accelerating the replacement of new epidermal cells. Rose essential oil, rich in terpenes and aromatic alcohols, is lipid-soluble and synergistic with petrolatum, enhancing the penetration of other active ingredients (such as Bacillus fermentation products) into the deeper layers of the skin, thus enhancing efficacy. Emulsifiers mix evenly with the aqueous phase to form a stable emulsion, improving application texture (e.g., smooth texture and easy spreading). Transdermal enhancers reduce skin barrier resistance, promoting the penetration of active ingredients such as Bacillus fermentation products and menthol into the dermis, where they directly act on frostbite lesions (e.g., dilating capillaries and inhibiting inflammatory factors). Therefore, the inventors combined these components to combat frostbite, enhancing its preventive and therapeutic effects. Furthermore, because these components exhibit anti-inflammatory, debridement, and skin-regenerating properties, they can achieve a long shelf life even without the addition of preservatives.

[0010] Furthermore, the emulsifier includes Span 60 and Tween 80, and the mass ratio of the two is 1:2.

[0011] Compared with the existing technology, the inventors found that when Span 60 and Tween 80 are used as emulsifiers and the mass ratio is 1:2, the two can significantly improve the emulsification stability, texture, skin feel and functional compatibility of skin care products through synergistic complementarity and molecular structure interaction through the HLB value (≈11.57).

[0012] Furthermore, the transdermal enhancer includes any one of azone or propylene glycol.

[0013] Compared to existing technologies, the inventors discovered that azone can interact with the skin's stratum corneum and lipids, lowering the phase transition temperature of active substances to the lipids in the interstices of the stratum corneum, increasing fluidity and reducing the diffusion resistance of drugs or active additives in the stratum corneum. This significantly enhances the penetration of active ingredients in skincare products, such as peppermint leaf extract and holly leaf extract, into the skin, allowing the anti-itch and anti-frostbite ingredients to better exert their effects. Propylene glycol has excellent solvent properties, helping other active ingredients penetrate deeper into the skin, improving the product's absorption and allowing the active ingredients in skincare products to better exert their anti-itch and anti-frostbite effects.

[0014] Furthermore, the skin care product also includes a preservative, and the mass fraction of the preservative is 0.1% to 0.2% based on the mass percentage of the skin care product.

[0015] Furthermore, the preservative includes any one of methyl parahydroxybenzoate, ethyl parahydroxybenzoate, and butyl parahydroxybenzoate.

[0016] The second aspect of the present invention provides a method for preparing the skin care product, comprising the following steps:

[0017] (1) Weighing the raw materials according to the mass percentage, then heating and melting the vaseline and emulsifier, and adding rose essential oil to obtain an oil phase system;

[0018] (2) dissolving the Bacillus fermentation product, mint leaf extract, holly leaf extract and transdermal enhancer in water and heating to obtain an aqueous phase system;

[0019] (3) Slowly adding the aqueous phase system to the oil phase system, homogenizing, cooling, and obtaining a skin care product.

[0020] Compared with the prior art, when preparing skin care products, the present invention first mixes petrolatum and an emulsifier to prepare an oil phase system; then mixes active ingredients to prepare an aqueous phase system; and finally, adds the aqueous phase to the oil phase to obtain an oil-in-water system. This system is beneficial for maintaining the stability of the properties of the skin care products and effectively locking in moisture.

[0021] Furthermore, in step (1), the heating and melting is heating the vaseline and emulsifier to 70°C to 75°C; and / or,

[0022] After adding rose essential oil, stir at 300rpm~500rpm for 10min~20min to mix evenly.

[0023] Compared to the prior art, the present invention sets the heating and melting temperature at 70°C to 75°C. This is because petrolatum, a major component of long-chain alkanes with a typical melting point of 46°C to 60°C, completely melts into a clear liquid when heated to 70°C to 75°C. Its low-viscosity fluidity facilitates uniform mixing with emulsifiers and rose essential oil. At 70°C to 75°C, petrolatum, as an oil-phase matrix, fully dissolves the fat-soluble components (such as terpenes) in rose essential oil, forming a uniform oil phase and preventing the essential oil from floating on the surface or forming oily spots due to excessively low temperatures. Span 60 is liquid at 70°C to 75°C, and its long-chain fatty acid ester groups tightly bind to the petrolatum's alkane chains through hydrophobic interactions, enhancing adsorption at the oil-phase interface. As a water-soluble emulsifier, Tween 80 can be fully dissolved in the aqueous phase at 70℃~75℃ and form a hydration layer. Its polyoxyethylene chain combines with water molecules through hydrogen bonds, and the ester end is entangled with the fatty acid chain of Span 60, forming a mixed liquid crystal structure at the oil-water interface.

[0024] Furthermore, in step (2), the heating is performed to 60°C to 70°C; after heating, the mixture is homogenized and stirred at 300 rpm to 500 rpm for 15 min to 25 min at 60°C to 70°C.

[0025] Compared to the prior art, the present invention heats the aqueous phase. On the one hand, the Bacillus fermentation product, peppermint leaf extract, wintergreen leaf extract, and transdermal enhancer need to be heated simultaneously to match the temperature of the oil phase to prevent sudden solidification of the emulsifier due to temperature differences during mixing, which could cause demulsification. On the other hand, the bioactive substances such as polypeptides and enzymes in the Bacillus fermentation product are easily degraded above 80°C, while 60°C to 70°C is in the medium temperature range, which can ensure the emulsification effect while maximizing the efficacy of the active ingredients. Therefore, heating the aqueous phase system ensures the stability of the system.

[0026] Furthermore, in step (3), the homogenization is carried out by stirring the mixture at 300 rpm to 500 rpm for 2 h to 3 h.

[0027] Compared with the existing technology, homogenous stirring at a speed of 300rpm~500rpm for 2h~3h can ensure that the emulsion system is uniform and stable.

[0028] Furthermore, the cooling is to 40°C to 45°C; and the cooling rate is 5°C to 10°C / h.

[0029] Compared with the existing technology, when the temperature is lowered to 40℃~45℃, the emulsion gradually solidifies, which can retain the anti-inflammatory and penetration-promoting effects to the maximum extent.

[0030] Furthermore, in step (3), the aqueous phase system is slowly added to the oil phase system at a rate of 1 mL / min-5 mL / min.

[0031] Furthermore, the skin care product prepared by the preparation method is in the form of an emulsion.

[0032] Compared with the existing technology, the skin care product is prepared into an emulsion dosage form, which is convenient for storage and transportation, and is convenient for application. DETAILED DESCRIPTION

[0033] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0034] Currently, many frostbite treatment products on the market contain irritating ingredients such as camphor and menthol, which can cause skin allergic reactions with long-term use. Vaseline, a classic skin moisturizer, has excellent occlusive and moisturizing properties, but using it alone has limited therapeutic effects on frostbite.

[0035] In order to overcome the above-mentioned drawbacks, an embodiment of the present invention provides a skin care product containing vaseline and having the effect of relieving itching and frostbite, which comprises the following ingredients in terms of mass percentage:

[0036] Vaseline 35%-55%, Bacillus fermentation product 3%-8%, peppermint leaf extract 1%-3%, holly leaf extract 2%-5%, rose essential oil 0.5%-1.5%, emulsifier 4%-10%, transdermal enhancer 0.5%-2.5% and appropriate amount of water.

[0037] It should be understood that "appropriate amount" here means that the sum of the mass fraction of water and the mass fractions of other components is 100%.

[0038] Compared with the prior art, the present invention uses petrolatum, Bacillus fermentation product, mint leaf extract, holly leaf extract, and rose essential oil as the main ingredients of the anti-itch and anti-frostbite skin care product, which can inhibit the release of histamine, reduce the inflammatory response in the frostbite area, and improve the prevention and treatment effect of frostbite. Moreover, because these ingredients have the effects of anti-inflammatory, removing dead tissue and promoting tissue regeneration when combined together, a longer shelf life can be achieved even without the addition of preservatives.

[0039] For example, the emulsifier includes Span 60 and Tween 80, and the mass ratio of the two is 1:2.

[0040] Compared with the existing technology, the inventors found that when Span 60 and Tween 80 are used as emulsifiers and the mass ratio is 1:2, the two significantly improve the emulsification stability, texture, skin feel and functional compatibility of skin care products through synergistic complementarity and molecular structure interaction through the HLB value (≈11.57).

[0041] For example, the skin penetration enhancer is azone;

[0042] An exemplary skin penetration enhancer is propylene glycol.

[0043] Compared to existing technologies, the inventors discovered that azone can interact with the skin's stratum corneum and lipids, lowering the phase transition temperature of active substances to the lipids in the interstices of the stratum corneum, increasing fluidity and reducing the diffusion resistance of drugs or active additives in the stratum corneum. This significantly enhances the penetration of active ingredients in skincare products, such as peppermint leaf extract and holly leaf extract, into the skin, allowing the anti-itch and anti-frostbite ingredients to better exert their effects. Propylene glycol has excellent solvent properties, helping other active ingredients penetrate deeper into the skin, improving the product's absorption and allowing the active ingredients in skincare products to better exert their anti-itch and anti-frostbite effects.

[0044] For example, the skin care product includes the following ingredients, calculated by mass percentage: 35% vaseline, 5% Bacillus fermentation product, 1% mint leaf extract, 2.5% holly leaf extract, 1.5% rose essential oil, 4% Span 60 and Tween 80, and the mass ratio of the two is 1:2, 0.5% azone and 50.5% water.

[0045] For example, the skin care product includes the following ingredients, calculated by mass percentage: 40% petrolatum, 3% Bacillus fermentation product, 3% mint leaf extract, 5% holly leaf extract, 1% rose essential oil, 6% Span 60 and Tween 80, and the mass ratio of the two is 1:2, 2.5% propylene glycol and 39.5% water.

[0046] For example, the skin care product includes the following ingredients, calculated by mass percentage: 55% petrolatum, 8% Bacillus fermentation product, 2% mint leaf extract, 3% holly leaf extract, 0.5% rose essential oil, 10% Span 60 and Tween 80, and the mass ratio of the two is 1:2, 1.5% azone and 20% water.

[0047] In some embodiments, the skin care product further comprises a preservative, and the mass fraction of the preservative is 0.1% to 0.2% based on the mass percentage of the skin care product;

[0048] For example, the mass fraction of the preservative can be selected as 0.1%, 0.12%, or 0.2%;

[0049] In some embodiments, the preservative can be specifically selected from any one of methylparaben, ethylparaben, and butylparaben.

[0050] A second aspect of an embodiment of the present invention provides a method for preparing a skin care product, comprising the following steps:

[0051] (1) Weighing the raw materials according to the mass percentage, then heating and melting the vaseline and emulsifier, and adding rose essential oil to obtain an oil phase system;

[0052] (2) dissolving the Bacillus fermentation product, mint leaf extract, holly leaf extract and transdermal enhancer in water and heating to obtain an aqueous phase system;

[0053] (3) Slowly adding the aqueous phase system to the oil phase system, homogenizing, cooling, and obtaining a skin care product.

[0054] Compared with the prior art, when preparing skin care products, the present invention first mixes petrolatum and an emulsifier to prepare an oil phase system; then mixes active ingredients to prepare an aqueous phase system; and finally, adds the aqueous phase to the oil phase to obtain an oil-in-water system. This system is beneficial for maintaining the stability of the properties of the skin care products and effectively locking in moisture.

[0055] In some embodiments, in step (1), the heating and melting is heating the vaseline and emulsifier to 70°C to 75°C;

[0056] For example, the specific selections are 72°C, 70°C, and 75°C;

[0057] For example, after adding rose essential oil, stir at 300 rpm to 500 rpm for 10 min to 20 min to mix evenly; for example, the rotation speed is specifically selected as 300 rpm, 400 rpm, and 500 rpm; the stirring time is specifically selected as 10 min, 15 min, and 20 min.

[0058] Compared with the prior art, the present invention sets the heating and melting temperature to 70° C. to 75° C., which is convenient for uniform mixing with the emulsifier and rose essential oil.

[0059] In some embodiments, in step (2), the heating is performed to 60°C to 70°C; after heating, the mixture is homogenized and stirred at 300 rpm to 500 rpm for 15 min to 25 min at 60°C to 70°C;

[0060] For example, the heating temperature can be specifically selected as 60° C., 70° C., or 65° C.; the rotation speed can be specifically selected as 300 rpm, 400 rpm, or 500 rpm; and the stirring time can be specifically selected as 20 min, 15 min, or 25 min.

[0061] Compared with the prior art, the present invention heats the aqueous phase, which not only ensures the emulsification effect but also maximizes the efficacy of the active ingredients. Therefore, heating the aqueous phase system ensures the stability of the system.

[0062] In some embodiments, in step (3), the homogenization is performed by stirring at 300 rpm to 500 rpm for 2 h to 3 h;

[0063] For example, the specific speeds can be selected as 300 rpm, 400 rpm, and 500 rpm; the specific homogenizing stirring time can be selected as 2 h, 3 h, and 2.5 h.

[0064] Compared with the existing technology, homogenizing and stirring at a speed of 300rpm~500rpm for 2h~3h ensures that the emulsion system is uniform and stable.

[0065] In some embodiments, the cooling is to 40°C~45°C; and the cooling rate is 5°C / h~10°C / h; wherein, the cooling temperature can be specifically selected as 40°C, 45°C, 42°C; the cooling rate can be specifically selected as 5°C / h, 10°C / h, 7°C / h.

[0066] Compared with the existing technology, when the temperature drops to 40℃~45℃, the emulsion gradually solidifies, retaining the anti-inflammatory and penetration-promoting effects to the maximum extent.

[0067] In some embodiments, in step (3), the aqueous phase system is slowly added to the oil phase system at a rate of 1 mL / min-5 mL / min;

[0068] For example, the rate can be selected as 1 mL / min, 5 mL / min, or 3 mL / min.

[0069] In some embodiments, the skin care product prepared by the preparation method is in the form of an emulsion.

[0070] Compared with the existing technology, the skin care product is prepared into an emulsion dosage form, which is convenient for storage and transportation, and is convenient for application.

[0071] In order to better illustrate the technical solution of the present invention, the present invention also provides the following specific examples. It should be understood that the raw materials used in the following examples are all commercially available raw materials unless otherwise specified.

[0072] Example 1

[0073] A skin care product containing vaseline and having the effects of relieving itching and removing frostbite comprises the following ingredients, calculated by weight percentage of the skin care product: 35% vaseline, 5% Bacillus fermentation product, 1% peppermint leaf extract, 2.5% holly leaf extract, 1.5% rose essential oil, 4% Span 60 and Tween 80, with the weight ratio of the two being 1:2, 0.5% azone and 50.5% water.

[0074] The preparation process of this skin care product is as follows:

[0075] (1) Weigh the raw materials according to the above mass percentages, then heat Vaseline, Span 60 and Tween 80 to 72°C to melt, add rose essential oil, and stir at 300 rpm for 15 minutes to obtain an oil phase system;

[0076] (2) The Bacillus fermentation product, mint leaf extract, holly leaf extract, and azone were dissolved in water, heated to 70°C, and homogenized and stirred at 300 rpm for 15 min to obtain an aqueous phase system;

[0077] (3) The aqueous phase system was slowly added to the oil phase system at a rate of 3 mL / min, homogenized at 300 rpm for 2.5 h, and cooled to 42 °C at a rate of 5 °C / h to obtain a skin care product.

[0078] Example 2

[0079] A skin care product containing vaseline and having the effects of relieving itching and removing frostbite comprises the following ingredients, calculated by weight percentage of the skin care product: 40% vaseline, 3% Bacillus fermentation product, 3% peppermint leaf extract, 5% holly leaf extract, 1% rose essential oil, 6% Span 60 and Tween 80, with the weight ratio of the two being 1:2, 2.5% propylene glycol and 39.5% water.

[0080] The preparation process of this skin care product is as follows:

[0081] (1) Weigh the raw materials according to the above mass percentages, then heat Vaseline, Span 60 and Tween 80 to 70°C to melt, add rose essential oil, and stir at 400 rpm for 20 minutes to obtain an oil phase system;

[0082] (2) The Bacillus fermentation product, mint leaf extract, holly leaf extract, and propylene glycol were dissolved in water, heated to 65°C, and homogenized and stirred at 400 rpm for 25 min to obtain an aqueous phase system;

[0083] (3) The aqueous phase system was slowly added to the oil phase system at a rate of 1 mL / min, homogenized at 400 rpm for 2 h, and cooled to 45 °C at a rate of 10 °C / h to obtain a skin care product.

[0084] Example 3

[0085] A skin care product containing vaseline and having the effects of relieving itching and removing frostbite comprises the following ingredients, calculated by weight percentage of the skin care product: 55% vaseline, 8% Bacillus fermentation product, 2% mint leaf extract, 3% holly leaf extract, 0.5% rose essential oil, 10% Span 60 and Tween 80, with the weight ratio of the two being 1:2, 1.5% azone and 20% water.

[0086] The preparation process of this skin care product is as follows:

[0087] (1) Weigh the raw materials according to the above mass percentages, then heat Vaseline, Span 60 and Tween 80 to 75°C to melt, add rose essential oil, and stir at 500 rpm for 10 minutes to obtain an oil phase system;

[0088] (2) The Bacillus fermentation product, mint leaf extract, holly leaf extract, and azone were dissolved in water, heated to 60°C, and homogenized and stirred at 500 rpm for 20 min to obtain an aqueous phase system;

[0089] (3) The aqueous phase system was slowly added to the oil phase system at a rate of 5 mL / min, homogenized at 500 rpm for 3 h, and cooled to 40 °C at a rate of 7 °C / h to obtain a skin care product.

[0090] Example 4

[0091] A skin care product containing vaseline and having the effects of relieving itching and removing frostbite comprises the following ingredients, calculated by weight percentage of the skin care product: 35% vaseline, 5% Bacillus fermentation product, 1% peppermint leaf extract, 2.5% holly leaf extract, 1.5% rose essential oil, 4% Span 60 and Tween 80, with the weight ratio of the two being 1:2, 0.5% azone, 0.1% methyl parahydroxybenzoate and 50.4% water.

[0092] The preparation process of this skin care product is as follows:

[0093] (1) Weigh the raw materials according to the above mass percentages, then heat Vaseline, Span 60 and Tween 80 to 72°C to melt, add rose essential oil, and stir at 300 rpm for 15 minutes to obtain an oil phase system;

[0094] (2) The Bacillus fermentation product, mint leaf extract, holly leaf extract, and azone were dissolved in water, heated to 70°C, and homogenized and stirred at 300 rpm for 15 min to obtain an aqueous phase system;

[0095] (3) The aqueous phase system was slowly added to the oil phase system at a rate of 3 mL / min, homogenized at 300 rpm for 2.5 h, methyl parahydroxybenzoate was added, and the temperature was lowered to 42 °C at a rate of 5 °C / h to obtain a skin care product.

[0096] Comparative Example 1

[0097] Compared with Example 1, the only difference is that the Bacillus fermentation product is replaced by an equal amount of vaseline, and the other components and proportions remain unchanged. The preparation method is the same as the preparation method of Example 1.

[0098] Comparative Example 2

[0099] Compared with Example 1, the only difference is that an equal amount of Bacillus fermentation product is used to replace vaseline, and the other components and proportions remain unchanged. The preparation method is the same as the preparation method of Example 1.

[0100] Comparative Example 3

[0101] Compared with Example 1, the only difference is that the mint leaf extract is replaced by an equal amount of Bacillus fermentation product, and the other components and proportions remain unchanged. The preparation method is the same as the preparation method of Example 1.

[0102] Comparative Example 4

[0103] Compared with Example 1, the only difference is that the holly leaf extract is replaced with an equal amount of Bacillus fermentation product, and the other components and proportions remain unchanged. The preparation method is the same as the preparation method of Example 1

[0104] Comparative Example 5

[0105] Compared with Example 1, the only difference is that the content of vaseline is reduced to 30%, the content of Bacillus fermentation product is increased to 15%, and the content of water is reduced to 45.5%.

[0106] Comparative Example 6

[0107] Compared with Example 1, the only difference is that the content of vaseline is increased by 60% and the content of water is reduced to 25.5%.

[0108] Evaluation of the effectiveness of frostbite treatment

[0109] Subject recruitment and grouping:

[0110] Recruitment of subjects will be conducted from December 24, 2023 to January 5, 2024. The target population is patients aged 18-60 years with mild to moderate frostbite (in accordance with the Diagnostic Criteria for Frostbite, WS / T 555-2017).

[0111] Exclusion criteria:

[0112] Allergic reaction to the test ingredients, severe frostbite with ulcers, recent use of steroid ointments

[0113] The grouping (n = 30 per group, male to female ratio 1:1) and the treatment methods of each group are shown in Table 1 ;

[0114] Table 1

[0115] Group Solution Example 1 group Smear Example 1 Example 4 Group Example 4 formulation containing preservatives Comparative Examples 1-6 Corresponding proportion formula Positive control group Commercially available 5% camphor frostbite cream (Brand A) Blank control group Petrolatum base (inactive ingredient)

[0116] (2) Experimental process

[0117] Baseline assessment (Day 0):

[0118] Record the frostbite area, erythema score (0-4 points), and itching degree (VAS 0-10 points)

[0119] Photographs were taken for archiving and telangiectasia was quantified using a dermatoscope (Dermlite DL4).

[0120] Intervention options:

[0121] Apply the test product twice a day (morning and evening), 0.5g each time. During use, do not use other frostbite treatment drugs.

[0122] Evaluation time point: retest on the 3rd, 7th and 14th days

[0123] The main efficacy indicators are shown in Table 2; the secondary efficacy indicators are shown in Table 3;

[0124] Table 2 Main efficacy indicators

[0125] index Detection method Erythema regression rate Skin colorimeter (Mexameter MX18) to measure a* value Itch relief rate Changes in VAS scores (patient self-assessment) Healing time When frostbite scabs / scaling first appeared

[0126] Table 3 Secondary efficacy indicators

[0127] index Detection method Skin barrier repair Transepidermal water loss (TEWL, AquaFlux AF200) Inflammatory factor levels Non-invasive skin sampling (tape stripping method) + ELISA detection of IL-6 / IL-1β

[0128] The comparison of frostbite symptom improvement (data on the 7th day) is shown in Table 4.

[0129] Table 4

[0130] Group Erythema regression rate Reduction in itching VAS Healing time (days) TEWL reduction value (g / m² / h) Example 1 78.5%* 6.2→1.8* 4.2* 38.2→22.1* Example 4 75.1%* 6.0→2.0* 4.5* 38.5→23.0* Comparative Example 1 48.7% 6.3→4.0 9.3 38.0→30.5 Comparative Example 2 50.8% 6.1→3.0 8.1 37.8→26.7 Comparative Example 3 65.4% 6.2→2.5* 5.5 38.1→25.3 Comparative Example 4 58.9% 6.0→3.2 6.9 37.9→28.6 Comparative Example 5 50.8% 6.3→3.8 7.8 39.0→32.0 Comparative Example 6 61.2% 6.1→3.5 6.2 36.5→34.2 Positive control 68.2% 6.0→2.3* 5.0 38.3→27.5 Blank control 30.5% 6.2→5.1 >14 37.8→36.0

[0131] (*p<0.05 vs blank control group, #p<0.01 vs positive control group, ANOVA+Tukey test)

[0132] As shown in Table 1, the combination of 35-40% petrolatum + 5% Bacillus in Examples 1 and 4 was the most effective, demonstrating the synergistic effect of oil phase closure and bioactive ingredients. The erythema resolution rate decreased in Comparative Example 1 (without Bacillus), and similarly decreased in Comparative Example 2 (without petrolatum), further confirming the synergistic effect of petrolatum and Bacillus. Comparative Example 3 (replacing peppermint leaf extract) still provided significant pruritus relief, suggesting that wintergreen leaf extract (methyl salicylate) played a primary role, but the composite plant extract in Example 1 was more effective. There was no significant difference between Example 4 and Example 1 (p>0.05), despite the preservative-containing solution (p>0.05), and 0.1% methylparaben had no effect on efficacy.

[0133] Shelf life test.

[0134] Test sample:

[0135] Examples 1 and 4 (with and without preservatives)

[0136] Comparative Example 1, Comparative Example 2, Comparative Example 5, Comparative Example 6 (key variable comparison)

[0137] Positive control: commercially available chilblain cream containing phenoxyethanol

[0138] Blank control: pure vaseline matrix

[0139] Test conditions:

[0140] Accelerated stability test (40°C ± 2°C, RH 75% ± 5%, 3 months ≈ 12 months at room temperature)

[0141] Long-term stability test (25°C ± 2°C, RH 60% ± 5%, 12 months)

[0142] The detection indicators are shown in Table 5.

[0143] Table 5

[0144] Test items method Eligibility criteria Microbial limits Microbial Limit Test Method for Non-Sterile Products in the 2020 Edition of the Chinese Pharmacopoeia Bacteria <100 CFU / g, mold / yeast <10 CFU / g Active ingredient retention rate HPLC (menthol, methyl salicylate) ≥90% initial content Physical stability Centrifugation (3000 rpm, 30 min), visual inspection No stratification, no crystallization

[0145] The experimental results are shown in Tables 6 to 8;

[0146] Table 6 Comparison of microbial limits (after 3 months of accelerated testing)

[0147] Group Bacteria (CFU / g) Mold / yeast (CFU / g) Whether it exceeds the standard Example 1 <10 Not detected no Example 4 <10 Not detected no Comparative Example 1 85 Not detected critical Comparative Example 5 110* 2 yes Positive control <10 Not detected no Blank control 35 Not detected no

[0148] Table 5 shows that even without the addition of preservatives, Example 1 still met the microbial standards. This is likely due to the natural antibacterial properties of the Bacillus fermentation product (its antimicrobial peptide, surfactin, inhibits Staphylococcus aureus, among others) and the low water activity environment (petroleum jelly content ≥ 35%), which limits microbial growth. However, in Comparative Example 5 (high water content + high fermentation content), bacterial levels exceeded the standard, demonstrating that a preservative is required when the petroleum jelly content is <35% to achieve the desired shelf life extension. This also demonstrates that combining petroleum jelly with Bacillus fermentation products can extend shelf life.

[0149] Table 7 Active ingredient retention rate (long-term test 12 months)

[0150] Group Menthol retention rate Methyl salicylate retention rate Rose essential oil retention rate Example 1 91.3% 93.5% 89.7% Example 4 92.1% 94.0% 90.2% Comparative Example 2 82.4% 85.1% 76.8% Positive control 88.5% 90.3% 84.5%

[0151] Table 8 Physical stability (centrifuge test)

[0152] Group Stratification Particle size change (D90) Example 1 none 4.8μm→5.1μm Example 4 none 4.7μm→5.0μm Comparative Example 6 Oil separation 4.9μm→12.3μm

[0153] It can be seen that when the petrolatum content is ≥35%, the emulsion structure is stable (the Span 60 / Tween 80 composite system can withstand centrifugation), while Comparative Example 6 (60% petrolatum) causes emulsion demulsification due to excessive oil phase.

[0154] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0155] The above description of the disclosed embodiments is intended to enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A skin care product containing vaseline and having the effect of relieving itching and removing frostbite, characterized in that: The skin care product comprises the following ingredients in percentage by mass: Vaseline 35%-55%, Bacillus fermentation product 3%-8%, peppermint leaf extract 1%-3%, holly leaf extract 2%-5%, rose essential oil 0.5%-1.5%, emulsifier 4%-10%, transdermal enhancer 0.5%-2.5% and appropriate amount of water.

2. The skin care product according to claim 1, characterized in that The emulsifier includes Span 60 and Tween 80, and the mass ratio of the two is 1:

2.

3. The skin care product according to claim 1, characterized in that The transdermal enhancer includes any one of azone or propylene glycol.

4. The skin care product according to claim 1, characterized in that It also includes a preservative, and the mass fraction of the preservative is 0.1% to 0.2% based on the mass percentage of the skin care product; Wherein, the preservative includes any one of methyl parahydroxybenzoate, ethyl parahydroxybenzoate and butyl parahydroxybenzoate.

5. The method for preparing the skin care product according to any one of claims 1 to 4, characterized in that: The steps include: (1) Weighing the raw materials according to the mass percentage, then heating and melting the vaseline and emulsifier, and adding rose essential oil to obtain an oil phase system; (2) dissolving the Bacillus fermentation product, mint leaf extract, holly leaf extract, and a transdermal enhancer in water, and heating the mixture to obtain an aqueous phase system; (3) Slowly adding the aqueous phase system to the oil phase system, homogenizing, cooling, and obtaining a skin care product.

6. The method for preparing the skin care product according to claim 5, wherein: In step (1), the heating and melting is heating the vaseline and emulsifier to 70°C to 75°C; and / or, After adding rose essential oil, stir at 300rpm~500rpm for 10min~20min to mix evenly.

7. The method for preparing the skin care product according to claim 5, characterized in that: In step (2), the heating is performed to 60°C to 70°C; after heating, the mixture is homogenized and stirred at 300 rpm to 500 rpm for 15 min to 25 min at 60°C to 70°C.

8. The method for preparing the skin care product according to claim 5, wherein: In step (3), the homogenization is carried out by stirring at 300 rpm to 500 rpm for 2 h to 3 h; and / or, The temperature is lowered to 40°C to 45°C; and the rate of the temperature reduction is 5°C / h to 10°C / h.

9. The method for preparing the skin care product according to claim 4, wherein: In step (3), the aqueous phase system is slowly added to the oil phase system at a rate of 1 mL / min-5 mL / min.

10. The method for preparing a skin care product according to any one of claims 5 to 9, characterized in that: The prepared skin care product is in the form of an emulsion.