Application of cassava leaf extract in promotion of chicken testis development

Abstract

The invention relates to the technical field of chicken reproductive development, in particular to application of a cassava leaf extract in promotion of chicken testis development. The application of the cassava leaf extract in promotion of chicken testis development provides a new thought and material basis for research in the field of chicken reproductive development. Meanwhile, the utilization value of the cassava leaves is expanded, the cassava leaf extract is applied to promotion of chicken testis development, the economic value of the cassava leaves can be further excavated, the application range of the cassava leaves in the breeding industry is widened, and meanwhile diversified development of the cassava industry is promoted. As a natural plant extract, the cassava leaf extract is wide in source, and the application of the cassava leaf extract in the field of reproductive development of chickens is beneficial to promoting the breeding industry to develop in a greener and sustainable direction.

Description

Technical Field

[0001] The invention relates to the technical field of chicken reproductive development, and in particular to application of cassava leaf extract in promoting chicken testicle development. Background Art

[0002] The chicken testis is the primary organ for producing sperm and male hormones. Its normal development and function significantly impact the reproductive efficiency of breeder chickens and the quality of their offspring. In practice, numerous factors, including nutrition, environment, and genetics, influence testicular development in chickens. Promoting testicular development can improve the reproductive efficiency of breeder chickens, increase the number of high-quality offspring, and thus enhance the overall economic benefits of chicken farms. Therefore, research on effective methods and substances for promoting testicular development in chickens has become a key component in improving the economic benefits of the poultry industry.

[0003] Cassava leaf extract (CLE), as a potential natural resource, is rich in bioactive substances, mainly flavonoids. These components play an important role in the growth, development, and physiological function regulation of animals. Cassava leaves have traditionally been used mainly for animal feed, but their potential bioactivity and functional value have not been fully explored. Invention patent publication number ID2021PID08426A discloses a method for extracting and purifying bioflavonoids from cassava leaves, which involves several stages starting with: drying and crushing the cassava leaves; extracting the cassava leaves with water or an alcohol-water mixture; filtering out the residual solids; passing the bioflavonoid extract through a resin column; eluting the bioflavonoids from the resin column with an alcohol solution; concentrating the bioflavonoid fraction; and crystallizing the bioflavonoids: filtering, washing, and drying the bioflavonoid crystals. In this invention, the cassava leaf bioflavonoids obtained contain rutin, nicotinoid, or a combination of the two, and are used to prepare cosmetic formulations in the form of sunscreens, night creams, and body lotions. Currently, in the field of animal nutrition, research on promoting reproductive organ development is still deepening, but there are no reports on the use of cassava leaf extract in promoting testicular development in chickens. Summary of the Invention

[0004] The present invention aims to provide an application of cassava leaf extract in promoting testicular development in chickens. The flavonoids in the cassava leaf extract have antioxidant effects. There are a large number of active cell metabolic processes in the testicular tissue, which easily produce excessive free radicals. Flavonoids are efficient antioxidants that can effectively scavenge free radicals and alleviate the damage of oxidative stress to testicular cells. In addition, flavonoids can regulate hormone levels in the human body or animal body, help maintain a good development state of the testicles, and promote the normal growth of the testicles and the performance of spermatogenic function. Therefore, extracting effective ingredients from cassava leaves and exploring their impact on chicken testicular development has important theoretical and practical value.

[0005] In order to achieve the above-mentioned object of the invention, the technical solution adopted by the present invention is:

[0006] Provided is the use of cassava leaf extract in promoting testicular development in chickens. The flavonoids in the cassava leaf extract include myricetin, rutin, nicotinoidin, and narcissin.

[0007] Furthermore, the preparation method of cassava leaf extract comprises the following steps:

[0008] The cassava leaf extract was then washed, dried, and ground. 50% ethanol was added and the mixture was soaked at 50°C for 2 hours, followed by discarding the waste liquid. This process was repeated three times. The mixture was centrifuged and filtered at 12,000 × g, and the residue was discarded. The supernatant was separated and purified, concentrated, spray-dried (70-75°C), and ground and sieved to remove impurities, thereby obtaining the cassava leaf extract.

[0009] Furthermore, 200 mg / kg-600 mg / kg of cassava leaf extract was added to the basal diet.

[0010] Furthermore, cassava leaf extract can increase the testicular weight, testicular diameter, area, number of spermatogenic stem cells in the testicles, and protein expression levels of testicular barrier function-related factors in chickens.

[0011] The beneficial effects of the present invention are:

[0012] The application of cassava leaf extract in promoting testicular development in chickens provides new insights and a material foundation for research in the field of chicken reproductive development. It also expands the utility of cassava leaves. Applying cassava leaf extract to promote testicular development in chickens can further tap into the economic value of cassava leaves, broaden their application in the aquaculture industry, and promote the diversified development of the cassava industry. As a natural plant extract with a wide range of sources, cassava leaf extract's application in the field of chicken reproductive development can help promote a greener and more sustainable aquaculture. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 Schematic diagram of the effects on testicular development of Wenchang chickens in the control group and the experimental group in Experimental Example 1; wherein, A: Wenchang chicken body weight at the end; B: Wenchang chicken testicular weight; C: testicular correction result; D: testicular picture taken on site; E: testicular diameter; F: seminiferous tubule area; G: testicular area; H: number of spermatogenic stem cells; I: testicular section result; J, K: testicular barrier function-related protein results; L, M: testicular C-kit immunofluorescence staining results. DETAILED DESCRIPTION

[0014] The specific embodiments of the present invention are described below to facilitate understanding of the present invention by those skilled in the art. However, it should be clear that the present invention is not limited to the scope of the specific embodiments. For those skilled in the art, as long as various changes are within the spirit and scope of the present invention as defined and determined by the appended claims, these changes are obvious, and all inventions and creations utilizing the concepts of the present invention are protected.

[0015] Test Example 1

[0016] This study used 480 one-day-old Wenchang chickens. The chickens were randomly divided into four groups of similar weight, with six replicates per group and 20 birds per replicate. The control group received a basal diet (CON), while the other three experimental groups received cassava leaf extract supplemented with the basal diet at 200 mg / kg (T1), 400 mg / kg (T2), and 600 mg / kg (T3), respectively. The experimental period lasted 30 days.

[0017] The cassava leaf extract production process involves washing, drying, and pulverizing the raw cassava. The extract is then soaked in 50% ethanol at 50°C for 2 hours, followed by discarding the waste liquid. This process is repeated three times. The extract is then centrifuged at 12,000 × g and filtered, with the residue discarded. The supernatant is then separated and purified, concentrated, spray-dried (70-75°C), and pulverized and sieved to remove impurities. The cassava leaf extract contains the following major flavonoids:

[0018] Table 1. Contents of main flavonoids in cassava leaf extract

[0019] flavonoids Myricetin Rutin nicotinoids Narcissusin Content (ug / g) 430.61 17186.37 5562.77 950.64

[0020] The experimental results are as follows Figure 1 As shown by Figure 1 From the results of group A, we can see that by designing different additive amounts of cassava leaf extract, it was found that adding 400 mg / kg cassava leaf extract (group T2) can significantly increase the body weight of Wenchang chicken, while there was no significant change in groups T1 and T3. Figure 1 The results in Figures B and C showed that the testicle weight of Wenchang chickens in group T2 increased significantly. Figure 1 Figure D in the middle shows that the testicle size of Wenchang chickens in the T2 group increased significantly. Figure 1 The results of EI showed that the diameter, area and number of spermatogenic stem cells in the testicles of Wenchang chickens in the T2 group were significantly higher than those in the CON group. Figure 1 The results in middle J and K showed that the expression levels of testicular barrier function proteins ZO-1, Occludin and Claudin-1 in Wenchang chickens in the T2 group were higher than those in the CON group. Figure 1 The results of L and M showed that the differentiation ability of Wenchang chicken sperm stem cells in the T2 group was stronger than that in the CON group.

[0021] In summary, the application of cassava leaf extract in promoting testicular development in chickens provides new insights and a material foundation for research in the field of chicken reproductive development. It also expands the utility of cassava leaves. Applying cassava leaf extract to promote testicular development in chickens can further tap into the leaf's economic value, broaden its application in the aquaculture industry, and promote the diversified development of the cassava industry. As a natural plant extract with a wide range of sources, cassava leaf extract's application in the field of chicken reproductive development can help promote a greener and more sustainable aquaculture.

[0022] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the present invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the present invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations that come within the meaning and range of equivalents of the claims be embraced therein.

[0023] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. The application of cassava leaf extract in promoting testicular development in chickens, characterized in that: The flavonoids in the cassava leaf extract include myricetin, rutin, nicotinoidin and narcissin.

2. The use of the cassava leaf extract in promoting testicular development in chickens according to claim 1, characterized in that The preparation method of the cassava leaf extract comprises the following steps: The cassava raw materials are washed, dried and crushed; 50% ethanol is added, and the mixture is soaked at 50°C for 2 hours, after which the waste liquid is discarded. This process is repeated multiple times, and the mixture is centrifuged and filtered at 12,000×g, and the filter residue is discarded. The supernatant is separated and purified, concentrated, spray-dried at 70-75°C, crushed and sieved to remove impurities, and thus a cassava leaf extract is obtained.

3. The use of the cassava leaf extract in promoting testicular development in chickens according to claim 1, characterized in that The cassava leaf extract was added to the basal diet at 400 mg / kg.

4. The use of the cassava leaf extract in promoting testicular development in chickens according to claim 1, characterized in that: The cassava leaf extract can increase the weight, diameter and area of chicken testicles, the number of spermatogonial stem cells in the testicles, and the protein expression level of factors related to testicular barrier function.

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