Plant powder for promoting peristalsis and defecation and preparation method thereof

Abstract

The invention provides plant powder for promoting intestinal peristalsis and defecation and a preparation method thereof, and belongs to the technical field of biology.The plant powder contains fructus momordicae, semen torreya extract, lemon balm extract, scaphium scaphigerum extract, fructus cannabis, semen cassiae, peach kernels, almonds, bunge cherry seeds, angelica sinensis, cistanche deserticola, prune and the like; the semen torreya extract, the lemon balm extract and the semen sterculiae lychnophorae extract are obtained by adopting a special preparation method. The components are matched according to a certain proportion for use, so that the composition has safe, efficient and lasting effects of promoting peristalsis and defecation, and constipation is not easy to relapse.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, and particularly relates to a plant powder for promoting intestinal peristalsis and defecation and a preparation method thereof. Background Art

[0002] In today's fast-paced world, people's eating habits and lifestyles have changed significantly. A high-fat, low-fiber diet, a sedentary work schedule, and chronic mental stress have led to increasingly prominent intestinal health issues, with constipation becoming a common ailment for many people. Constipation not only causes immediate pain, such as abdominal distension and discomfort, but also negatively impacts overall health. Long-term constipation causes harmful substances to accumulate in the intestines, preventing them from being excreted promptly. These toxins are then reabsorbed by the intestines, potentially causing skin problems such as dull skin, acne, and dark spots. It also increases the metabolic burden on organs like the liver and kidneys, increasing the risk of cardiovascular and cerebrovascular diseases such as hypertension and coronary heart disease.

[0003] Currently, there are a wide variety of solutions and products available on the market for constipation, but all have limitations. Common medications, such as stimulant laxatives, can promote bowel movements in the short term. However, long-term use can lead to intestinal mucosal damage, intestinal nerve dysfunction, and intestinal dependence on the medication, creating a so-called "laxative colon" that further exacerbates constipation symptoms. While relatively mild, bulk laxatives require a large amount of water to work and are slow to take effect. For busy modern people, strict adherence to these laxatives is difficult and can result in poor compliance.

[0004] While dietary recommendations like increasing dietary fiber intake and drinking more water can help improve intestinal function, in practice, achieving the desired results is often difficult due to difficulties changing dietary habits and a fast-paced lifestyle that prevents people from maintaining a regular diet. Furthermore, while some health supplements on the market claim to promote intestinal motility, most are ineffective, leading to recurrence of constipation and failing to fundamentally address the issues of insufficient intestinal motility and difficulty in defecation.

[0005] Furthermore, with increasing health awareness, consumers are placing higher demands on the safety and effectiveness of intestinal health products. Consumers are increasingly choosing products that are natural, side-effect-free, long-lasting, and can fundamentally improve intestinal function. Therefore, the development of safe, effective, and long-lasting products that promote bowel movements is of great practical significance and market demand. Summary of the Invention

[0006] In view of the fact that the existing market still lacks effective substances that are safe, efficient, and long-lasting intestinal motility and defecation promotion, the present invention provides a plant powder for promoting intestinal motility and defecation and a preparation method thereof. The plant powder contains ingredients such as Momordica grosvenori, Torreya grandiflora extract, lemon balm extract, Sterculia lychnophora extract, Hemp seed, Cassia seed, Peach kernel, Apricot kernel, Prunus mume seed, Angelica sinensis, Cistanche deserticola, and Prunus citriodora extract; Torreya grandiflora extract, lemon balm extract, and Sterculia lychnophora extract are obtained by a special preparation method. The ingredients are used in combination in a certain proportion to have a safe, efficient, and long-lasting effect of promoting intestinal motility and defecation, and constipation is not likely to recur. The specific technical scheme is as follows: A plant powder for promoting intestinal peristalsis and defecation comprises the following raw materials in parts by mass: 5 to 10 parts of monk fruit, 3 to 6 parts of torreya grandis extract, 4 to 8 parts of lemon balm extract, 2 to 5 parts of sterculia lychnophora extract, 3 to 6 parts of hemp seed, 5 to 10 parts of cassia seed, 2 to 5 parts of peach kernel, 2 to 5 parts of apricot kernel, 2 to 5 parts of Prunus mume seed, 2 to 5 parts of angelica sinensis, 1 to 3 parts of Cistanche deserticola and 1 to 3 parts of prune.

[0007] The torreya grandis extract comprises an enzymatic hydrolysate obtained by sequentially enzymolysis of torreya grandis kernels with lipase and alkaline protease, a product obtained by twice extracting the enzymatic hydrolysate with supercritical CO2 dynamic countercurrent, and a product below 5 kDa obtained by ultrafiltration of the remaining enzymatic hydrolysate with an ultrafiltration membrane.

[0008] The lemon balm extract comprises a product obtained by hydrolyzing the lemon balm with hemicellulase and pectinase, and then hydrolyzing it with bromelain and serrapeptase in sequence.

[0009] The sterculia lychnophora extract comprises an enzymatic hydrolyzate obtained by ultrasonically hydrolyzing sterculia lychnophora seeds with hemicellulase, xylanase and papain, a product obtained by subjecting the enzymatic hydrolyzate to supercritical CO2 dynamic countercurrent extraction, and a product below 5 kDa obtained by ultrafiltration of the remaining enzymatic hydrolyzate through an ultrafiltration membrane.

[0010] In the above-mentioned plant powder, the preparation of the ginkgo nut extract includes: quick freezing the ginkgo nut kernels with liquid nitrogen, crushing to obtain ginkgo nut kernel powder; according to the mass ratio, the ginkgo nut kernel powder: water: lipase: alkaline protease = 1: (10-12): (0.01-0.015): (0.006-0.01), mixing the powder, water and lipase, adjusting the pH, and enzymolysis, then adjusting the pH and adding alkaline protease, enzymolysis to obtain an enzymatic solution; the enzymatic solution is subjected to supercritical CO2 dynamic countercurrent primary extraction to obtain a primary extract; the remaining enzymatic solution is subjected to supercritical CO2 dynamic countercurrent secondary extraction to obtain a secondary extract, and the primary extract and the secondary extract are combined to obtain an extract; the remaining enzymatic solution is ultrafiltered using an ultrafiltration membrane to obtain a filtrate, the filtrate is combined with the extract, concentrated and dried to obtain the ginkgo nut extract.

[0011] In the above-mentioned plant powder, the preparation method of the Torreya grandis extract comprises the following steps: quick-freezing Torreya grandis kernels with liquid nitrogen and crushing to obtain Torreya grandis kernel powder; mixing the powder, water and lipase in a mass ratio of Torreya grandis kernel powder: water: lipase: alkaline protease = 1: (10-12): (0.01-0.015): (0.006-0.01), adjusting the pH value to 6.5-7.5, performing enzymatic hydrolysis at 42-48°C for 2.5h-3.5h, then adjusting the pH value to 8.0-9.0, adding alkaline protease, and incubating at 52-5 The enzymatic hydrolysis was carried out at 7°C for 1.5 h to 2.5 h to obtain an enzymatic hydrolysate; the enzymatic hydrolysate was subjected to a primary extraction with supercritical CO2 dynamic countercurrent to obtain a primary extract containing β-sitosterol and torreya esters; the remaining enzymatic hydrolysate was subjected to a secondary extraction with supercritical CO2 dynamic countercurrent to obtain a secondary extract containing torreya polyphenols and flavonoid glycosides, the primary extract and the secondary extract were combined to obtain an extract; the remaining enzymatic hydrolysate was ultrafiltered using a 5 kDa ultrafiltration membrane to obtain a filtrate less than 5 kDa, the filtrate was combined with the extract, and the mixture was concentrated and dried below 50°C to a constant weight to obtain a torreya nut extract.

[0012] In the above-mentioned preparation method of the Torreya grandis extract, the liquid nitrogen quick-freezing time is 3 minutes to 5 minutes; the crushing particle size is through an 80-mesh to 120-mesh sieve; the parameters of the supercritical CO2 dynamic countercurrent primary extraction are: pressure 22MPa to 28MPa, temperature 42°C to 48°C, CO2 flow rate 12L / h to 18L / h, time 2h to 3h; the parameters of the supercritical CO2 dynamic countercurrent secondary extraction are: pressure 32MPa to 38MPa, temperature 52°C to 58°C, entrainer ethanol ratio 4% v / v to 6% v / v, flow rate 12L / h to 18L / h, time 2h to 3h.

[0013] In the above-mentioned plant powder, the preparation of the lemon balm extract includes: crushing the lemon balm, adding water, then adding 0.3% to 0.5% of hemicellulase and 0.1% to 0.3% of pectinase by weight of the lemon balm, adjusting the pH value for enzymatic hydrolysis, then adjusting the pH value, adding 0.5% to 1% of bromelain by weight of the lemon balm, enzymatic hydrolysis, and heating to inactivate the enzyme; after cooling, adjusting the pH value, adding 0.5% to 1.0% of serrapeptase by weight of the lemon balm, enzymatic hydrolysis, and heating to inactivate the enzyme; after cooling, filtering, concentrating the filtrate, and drying to obtain the lemon balm extract.

[0014] In the above-mentioned plant powder, the preparation method of the lemon balm extract comprises the following steps: taking the whole plant of lemon balm and crushing it, adding 8 to 12 times the mass of lemon balm water, then adding 0.3% to 0.5% of hemicellulase and 0.1% to 0.3% of pectinase by mass of lemon balm, adjusting the pH value to 4.2 to 4.8, enzymolysis at 40°C to 50°C for 90min to 150min, then adjusting the pH value to 6.0 to 7.0, adding lemon Bromelain at a concentration of 0.5% to 1% by weight of the lemon balm is enzymatically hydrolyzed at 50°C to 55°C for 60 to 90 minutes, and the temperature is increased to inactivate the enzyme; after cooling to room temperature, the pH is adjusted to 7.0 to 8.0, and serrapeptase at a concentration of 0.5% to 1.0% by weight of the lemon balm is added, and the enzymatic hydrolysis is carried out at 35°C to 40°C for 60 to 90 minutes, and the temperature is increased to inactivate the enzyme; after cooling to room temperature, the solution is filtered through a mesh, the filtrate is collected, and the solution is concentrated under reduced pressure at low temperature and dried at low temperature to a constant weight to obtain a lemon balm extract.

[0015] In the preparation method of the lemon balm extract, the temperature for heating and inactivating the enzyme is 80°C to 85°C, and the time for heating and inactivating the enzyme is 10 minutes to 15 minutes; the mesh size of the sieve is 250 mesh to 325 mesh; the temperature for low-temperature reduced pressure concentration is below 60°C; and the temperature for low-temperature drying is below 60°C.

[0016] In the above-mentioned plant powder, the preparation of the sterculia lychnophora extract includes: crushing the sterculia lychnophora seeds, adding water, then adding 0.5% to 0.7% of hemicellulase, 0.3% to 0.5% of xylanase and 0.5% to 1.0% of papain based on the mass of the sterculia lychnophora seeds, ultrasonic enzymolysis to obtain ultrasonic enzymatic hydrolyzate, supercritical CO2 dynamic countercurrent extraction to obtain an extract; the remaining enzymatic hydrolyzate is ultrafiltered through an ultrafiltration membrane to obtain a filtrate, the filtrate is combined with the extract, concentrated and dried to obtain the sterculia lychnophora extract.

[0017] In the above-mentioned plant powder, the preparation method of the sterculia lychnophora extract includes the following steps: taking sterculia lychnophora seeds and crushing them, adding water 10 times to 12 times the mass of the sterculia lychnophora seeds, and then adding 0.5% to 0.7% of hemicellulase, 0.3% to 0.5% of xylanase and 0.5% to 1.0% of papain by mass of the sterculia lychnophora seeds, and ultrasonically enzymolyzing them at a pH of 5.5 to 6.0 and 45°C to 55°C to obtain an ultrasonic hydrolyzate; the ultrasonic hydrolyzate is subjected to dynamic countercurrent extraction with supercritical CO2 to obtain an extract; the remaining hydrolyzate is ultrafiltered using a 5kDa ultrafiltration membrane to obtain a filtrate less than 5kDa, the filtrate is combined with the extract, and concentrated and dried below 50°C to constant weight to obtain a sterculia lychnophora extract.

[0018] In the preparation method of the above-mentioned Sterculia lychnophora extract, the frequency of the ultrasound is 20kHz~25kHz, and the time of the ultrasound is 1h~2h; the extraction parameters are: pressure 22MPa~28MPa, temperature 42℃~48℃, CO2 flow rate 12L / h~18L / h, entrainer ethanol ratio 4%v / v~6%v / v, time 2h~3h.

[0019] In the above-mentioned plant powder, the Momordica grosvenori, hemp seed, cassia seed, peach kernel, apricot kernel, Prunus mume seed, angelica sinensis, Cistanche deserticola and prune are all ground medicinal materials into dry powder.

[0020] The method for preparing the above-mentioned plant powder for promoting intestinal peristalsis and defecation comprises the following steps: Separately sieve the following: Momordica grosvenori, Torreya grandiflora extract, lemon balm extract, Sterculia lychnophora extract, Cannabis sativa seed, Cassia seed, peach kernel, apricot kernel, Prunus armeniaca seed, Angelica sinensis, Cistanche deserticola and Prunus citriodora, take the sieve underflow, mix them uniformly by weight to obtain a mixture, add 5% to 10% by weight of starch slurry of the mixture, stir uniformly, and granulate with a rocking granulator and sieve with a 30-35 mesh sieve to obtain granular powder, dry it to control the moisture content of the granular powder to 4wt% to 6wt%, put the dried granules into the mixer again, and mix them for 10min to 15min to obtain a plant powder.

[0021] In the above preparation method, the starch concentration of the starch slurry is 10wt% to 20wt%; the drying temperature is 50°C to 60°C, and the drying time is 2h to 4h.

[0022] The plant powder can be used to prepare food, health products or medicines.

[0023] The plant powder for promoting intestinal peristalsis and defecation and the preparation method thereof of the present invention have the following beneficial effects: 1. The plant powder of the present invention contains ingredients such as Momordica grosvenori, Torreya grandiflora extract, lemon balm extract, Sterculia lychnophora extract, Hemp seed, Cassia seed, Peach kernel, Apricot kernel, Prunus mume seed, Angelica sinensis, Cistanche deserticola, and Prunus citriodora. Among them, Torreya grandiflora extract contains β-sitosterol, Torreya ester, Torreya grandiflora polyphenols, flavonoid glycosides, and small molecule peptides. β-sitosterol can competitively inhibit the intestinal absorption of cholesterol, reduce the cholesterol content in the intestine, and reduce the deposition of fat in the intestinal wall, thereby improving the lipid environment of the intestine and making intestinal peristalsis smoother. Torreya ester has the effect of lubricating the intestine, which can reduce the friction between feces and the intestinal wall, making feces easier to pass through the intestine. Torreya grandis polyphenols and flavonoid glycosides possess strong antioxidant capacity, scavenging free radicals in the intestines, protecting the intestinal mucosa from oxidative damage, and maintaining its integrity and normal function, thereby promoting intestinal motility and defecation. Small molecule peptides participate in the signal transduction process of intestinal cells, regulating the contraction and relaxation of intestinal smooth muscle, further enhancing intestinal motility and aiding in the excretion of feces. The active ingredients in lemon balm extract are primarily volatile oils and some unknown bioactive substances, which can stimulate nerve endings in the intestine, causing the contraction and relaxation of intestinal smooth muscle and promoting intestinal motility. They can also regulate the function of the intestinal nervous system, relieve intestinal spasms, and make intestinal motility more regular and coordinated, facilitating the normal excretion of feces. Plantago ovata extract is rich in polysaccharides, polypeptides and other ingredients; polysaccharides have strong water absorption and can absorb a large amount of water in the intestine, causing the volume of feces to expand, thereby stimulating the receptors of the intestinal wall, causing intestinal peristaltic reflex, and promoting intestinal peristalsis; in addition, polysaccharides can also increase the secretion of intestinal mucus, forming a lubricating protective film on the surface of the intestinal mucosa, reducing the friction between feces and the intestinal wall, and making feces easier to excrete from the body; polypeptides can participate in the signal transduction process of intestinal cells, regulate the contraction and relaxation of intestinal smooth muscles, further enhance the motility of the intestine, and help feces to be excreted from the body.

[0024] Second, hemp seed contains a large amount of oil, mainly unsaturated fatty acids such as linolenic acid and linoleic acid. These oils can act as lubricants in the intestines, making it easier for feces to pass through the intestines. At the same time, unsaturated fatty acids can regulate the membrane fluidity of intestinal cells, affect the ion channels and signal transduction pathways on the cell membrane, enhance the contraction ability of intestinal smooth muscle, and promote intestinal peristalsis. In addition, hemp seed also contains some protein and dietary fiber. Protein can provide nutrition to intestinal cells and maintain normal physiological functions of the intestine, while dietary fiber can increase fecal volume and promote intestinal peristalsis. The main active ingredients of Cassia seed are anthraquinones, such as chrysophanol and emodin. These anthraquinones can stimulate the intestinal mucosa, causing it to secrete more digestive fluid and increase intestinal lubrication. At the same time, anthraquinones can also act on intestinal smooth muscle, promoting its contraction, enhancing intestinal peristalsis, and promoting the excretion of feces. Peach kernel, apricot kernel, and Prunus mume kernel: These three kernel-based herbs are rich in oil, which acts as a lubricant in the intestines, softening stool, reducing its hardness, and making it easier to pass. They also contain ingredients like amygdalin, which, when broken down by microorganisms in the intestines, produces hydrocyanic acid, which inhibits the respiratory center, reflexively causing increased intestinal motility and promoting defecation. The main components of angelica include volatile oils, polysaccharides, and ferulic acid. Volatile oils can stimulate and dilate intestinal blood vessels, increasing blood supply to the intestines and providing more energy for intestinal motility. Polysaccharides can regulate intestinal flora, promoting the growth of beneficial bacteria, inhibiting the reproduction of harmful bacteria, and maintaining a balanced intestinal microecology, thereby enhancing digestion and absorption and promoting intestinal motility. Ferulic acid has antioxidant and anti-inflammatory properties, protecting the intestinal mucosa from damage, maintaining normal function, and promoting intestinal motility and defecation. Cistanche deserticola contains phenylethanoid glycosides and polysaccharides. Phenylethanoid glycosides can promote the secretion of intestinal mucus, increase intestinal lubrication, and make stool easier to pass. Polysaccharides can regulate intestinal flora, enhance intestinal immunity, promote the growth of beneficial intestinal bacteria, inhibit the growth of harmful bacteria, and maintain the balance of the intestinal microecology. At the same time, polysaccharides can absorb water, increase the volume of intestinal contents, stimulate intestinal peristalsis, and promote defecation. Prunes are rich in dietary fiber and sorbitol. Dietary fiber is divided into soluble dietary fiber and insoluble dietary fiber. Soluble dietary fiber forms a sticky substance in the intestines, increasing the volume and moisture of stool and promoting intestinal peristalsis. Insoluble dietary fiber increases the weight of stool and stimulates intestinal peristalsis. Sorbitol is a sugar alcohol that is not easily absorbed in the intestines. It can retain moisture in the intestines, soften stool, and stimulate intestinal peristalsis to promote defecation.

[0025] Second, the specific ratio of the compatibility of the present invention is carefully designed and has many advantages. First, from the perspective of lubricating the intestine, the oil-rich ingredients such as hemp seed, peach kernel, apricot kernel, and Prunus mume kernel, combined in a certain proportion, can provide sufficient lubrication to ensure that feces can move smoothly in the intestine. At the same time, the ingredients in Sterculia lychnophora extract and prunes increase the volume of feces and retain moisture, which cooperates with the lubricating effect of oil to prevent feces from being too dry to be discharged, nor from being difficult to discharge due to lack of lubrication.

[0026] From the perspective of promoting intestinal peristalsis, the anthraquinones in Cassia seed stimulate the contraction of intestinal smooth muscle, lemon balm extract regulates the intestinal nervous system to make peristalsis more regular, and combined with angelica, it promotes intestinal blood circulation to provide energy for peristalsis. These ingredients work synergistically to enhance the peristaltic ability of the intestine from multiple levels.

[0027] Furthermore, Momordica grosvenori and Cistanche deserticola regulate the intestinal flora, providing a healthy microecological environment for intestinal health and further promoting the other ingredients to promote bowel movements and defecation. This multi-target, multi-pathway synergistic effect avoids the side effects of excessive use of a single ingredient while fully leveraging the advantages of each ingredient, making the plant powder more effective in promoting bowel movements and defecation.

[0028] 3. In the preparation method of Torreya grandis extract, multi-enzyme step-by-step enzymatic hydrolysis and supercritical CO2 two-stage extraction are combined with ultrafiltration: first, lipase and alkaline protease are used for step-by-step enzymatic hydrolysis under different pH values and temperature conditions to fully extract components such as protein and fat in Torreya grandis. Then, through supercritical CO2 dynamic countercurrent primary extraction and secondary extraction, the primary extract containing β-sitosterol and Torreya grandis ester and the secondary extract containing Torreya grandis polyphenols and flavonoid glycosides are extracted under different pressure, temperature and entrainer conditions. Finally, the remaining enzymatic hydrolyzate is ultrafiltered using a 5kDa ultrafiltration membrane to obtain a filtrate less than 5kDa, which contains characteristic small molecule peptides. The Torreya grandis extract prepared by the above innovative process contains a variety of effective ingredients. The synergistic effect of multiple ingredients makes the Torreya grandis extract have significant advantages in promoting intestinal peristalsis and defecation.

[0029] Fifth, the preparation method for lemon balm extract utilizes multiple enzymes, including hemicellulase, pectinase, bromelain, and serrapeptase, to perform multiple enzymatic hydrolysis cycles at varying pH and temperature conditions. This method leverages the specific characteristics of different enzymes to gradually decompose the various components in lemon balm, improving the extraction rate of active ingredients and yielding specific small-molecule peptides and other components. The lemon balm extract obtained through multiple enzymatic hydrolysis is rich in active ingredients, promotes intestinal health and motility, and synergizes with volatile oils.

[0030] 6. In the preparation method of Sterculia lychnophora extract, ultrasonic enzymatic hydrolysis is combined with supercritical CO2 extraction and ultrafiltration. This process combines a variety of advanced extraction and separation technologies, which can achieve efficient extraction and purification of different types of active ingredients in Sterculia lychnophora, obtain rich active ingredients, and make it have better effects in promoting intestinal peristalsis and defecation.

[0031] 7. Monk fruit primarily promotes bowel movements by regulating intestinal flora and enhancing intestinal motility, while Torreya grandis extract focuses on regulating intestinal lipid metabolism and lubricating the intestines. When the two are combined in a specific ratio, they can exert a synergistic effect.

[0032] 8. Lemon balm extract primarily stimulates intestinal nerves and smooth muscles, regulating the intestinal nervous system to promote intestinal motility and make it more regular. Sterculia lychnophora extract, on the other hand, primarily promotes bowel movements by increasing stool bulk and lubricating the intestines. When the two are combined in a specific ratio, lemon balm extract stimulates the contraction and relaxation of intestinal smooth muscle, while Sterculia lychnophora extract stimulates intestinal wall receptors due to the expansion of stool, inducing an intestinal peristaltic reflex. The two work synergistically to enhance intestinal motility through different mechanisms. Regarding improving bowel movements, lemon balm extract regulates the intestinal nervous system, relieving intestinal spasms and making intestinal motility more coordinated. Sterculia lychnophora extract increases intestinal mucus secretion, lubricates the intestines, and reduces friction between stool and the intestinal wall. Together, these two effects make stool easier to pass. Taken together, this combination can synergistically promote intestinal motility and bowel movements, improving bowel movement efficiency. DETAILED DESCRIPTION

[0033] The present invention will be further described below with reference to specific implementation cases, but the present invention is not limited to these embodiments.

[0034] Example 1 A plant powder for promoting intestinal peristalsis and defecation, comprising the following raw materials in parts by mass: 7 parts of monk fruit, 4.5 parts of torreya grandis extract, 6 parts of lemon balm extract, 3.5 parts of sterculia lychnophora extract, 4 parts of hemp seed, 8 parts of cassia seed, 3 parts of peach kernel, 3 parts of apricot kernel, 4 parts of Prunus mume kernel, 3 parts of angelica sinensis, 2 parts of Cistanche deserticola, and 2 parts of prune.

[0035] The preparation method of the ginkgo nut extract includes the following steps: quick-freezing the ginkgo nut kernels with liquid nitrogen for 4 minutes, crushing them, and passing them through a 100-mesh sieve to obtain ginkgo nut kernel powder; mixing the powder, water and lipase in a mass ratio of ginkgo nut kernel powder: water: lipase: alkaline protease = 1:11:0.012:0.008, adjusting the pH value to 7.0, and performing enzymatic hydrolysis at 46°C for 3 hours, then adjusting the pH value to 8.5, adding alkaline protease, and performing enzymatic hydrolysis at 55°C for 2 hours to obtain an enzymatic solution; subjecting the enzymatic solution to a supercritical CO2 dynamic countercurrent primary extraction, and the parameters of the primary extraction are: pressure 25MPa, temperature 45°C, CO2 flow The primary extract containing β-sitosterol and torreya ester was obtained at a flow rate of 15 L / h and a time of 2.5 h. The remaining enzymatic hydrolysate was subjected to a supercritical CO2 dynamic countercurrent secondary extraction. The parameters of the secondary extraction were: pressure 35 MPa, temperature 55°C, entrainer ethanol ratio 5% v / v, flow rate 15 L / h, and time 2.5 h. A secondary extract containing torreya polyphenols and flavonoid glycosides was obtained. The primary extract and the secondary extract were combined to obtain an extract. The remaining enzymatic hydrolysate was ultrafiltered using a 5 kDa ultrafiltration membrane to obtain a filtrate less than 5 kDa. The filtrate was combined with the extract, concentrated and dried at 44°C to constant weight to obtain a torreya nut extract.

[0036] The preparation method of lemon balm extract includes the following steps: taking the whole plant of lemon balm and crushing it, adding water 10 times the mass of lemon balm, then adding 0.4% hemicellulase and 0.2% pectinase by weight of lemon balm, adjusting the pH value to 4.5, enzymatically hydrolyzing at 45°C for 120 minutes, then adjusting the pH value to 6.5, adding 0.7% bromelain by weight of lemon balm, enzymatically hydrolyzing at 52°C for 80 minutes, heating to 82°C to inactivate the enzyme for 12 minutes; after cooling to room temperature, adjusting the pH value to 7.5, adding 0.8% serrapeptase by weight of lemon balm, enzymatically hydrolyzing at 38°C for 80 minutes, heating to 82°C to inactivate the enzyme for 12 minutes; after cooling to room temperature, filtering with a 250-mesh sieve, taking the filtrate, concentrating under reduced pressure at 54°C, and drying at 54°C to constant weight to obtain the lemon balm extract.

[0037] Among them, the preparation method of the sterculia lychnophora extract includes the following steps: taking sterculia lychnophora seeds and crushing them, adding water 11 times the mass of the sterculia lychnophora seeds, and then adding 0.6% hemicellulase, 0.4% xylanase and 0.8% papain of the mass of the sterculia lychnophora seeds, and under the conditions of pH 5.8 and 50°C, ultrasonic enzymolysis is performed at 22kHz for 1.5h to obtain an ultrasonic hydrolyzate; the ultrasonic hydrolyzate is subjected to supercritical CO2 dynamic countercurrent extraction, and the extraction parameters are: pressure 25MPa, temperature 45°C, CO2 flow rate 15L / h, entrainer ethanol ratio 5%v / v, time 2.5h to obtain an extract, and the remaining enzymatic hydrolyzate is ultrafiltered using a 5kDa ultrafiltration membrane to obtain a filtrate less than 5kDa, the filtrate is combined with the extract, and concentrated and dried at 44°C to constant weight to obtain the sterculia lychnophora extract.

[0038] Among them, hemp seed, cassia seed, peach kernel, apricot kernel, Prunus mume seed, angelica, Cistanche deserticola and prune are all crushed medicinal materials into dry powder.

[0039] The method for preparing the above-mentioned plant powder for promoting intestinal peristalsis and defecation comprises the following steps: Separately sieve the extracts of Momordica grosvenori, Torreya grandiflora, lemon balm, Sterculia lychnophora, Fructus Hemp, Semen Cassiae, Peach Kernel, Apricot Kernel, Prunus Amygdalus, Semen Euphorbiae, Herba Cistanches and Prunus Western Berry through an 80-mesh sieve, take the sieve-less materials, mix them uniformly by weight to obtain a mixture, add 15wt% starch slurry which is 8% by weight of the mixture, stir uniformly, and granulate with a rocking granulator and a 30-mesh sieve to obtain granular powder, dry it at 55°C for 3h to control the moisture content of the granular powder to 5wt%, put the dried granules into the mixer again and mix for 12min to obtain a plant powder.

[0040] Example 2 A plant powder for promoting intestinal peristalsis and defecation, comprising the following raw materials in parts by mass: 5 parts of monk fruit, 3 parts of torreya grandis extract, 4 parts of lemon balm extract, 2 parts of sterculia lychnophora extract, 3 parts of hemp seed, 5 parts of cassia seed, 2 parts of peach kernel, 2 parts of apricot kernel, 2 parts of Prunus mume seed, 2 parts of angelica sinensis, 1 part of Cistanche deserticola and 1 part of prune.

[0041] The preparation method of the ginkgo nut extract comprises the following steps: quick-freezing the ginkgo nut kernels with liquid nitrogen for 3 minutes, crushing them, and passing them through an 80-mesh sieve to obtain ginkgo nut kernel powder; mixing the ginkgo nut kernel powder: water: lipase: alkaline protease in a mass ratio of 1:10:0.01:0.006, adjusting the pH value to 6.5, performing enzymatic hydrolysis at 42°C for 2.5 hours, then adjusting the pH value to 8.0, adding alkaline protease, and performing enzymatic hydrolysis at 52°C for 1.5 hours to obtain an enzymatic solution; subjecting the enzymatic solution to a supercritical CO2 dynamic countercurrent primary extraction, wherein the parameters of the primary extraction are: pressure 22 MPa, temperature 42°C, C The O2 flow rate was 12 L / h, and the time was 2 h to obtain a primary extract containing β-sitosterol and torreya esters; the remaining enzymatic hydrolysate was subjected to supercritical CO2 dynamic countercurrent secondary extraction, and the parameters of the secondary extraction were: pressure 32 MPa, temperature 52°C, entrainer ethanol ratio 4% v / v, flow rate 12 L / h, and time 2 h to obtain a secondary extract containing torreya polyphenols and flavonoid glycosides. The primary extract and the secondary extract were combined to obtain an extract; the remaining enzymatic hydrolysate was ultrafiltered using a 5 kDa ultrafiltration membrane to obtain a filtrate less than 5 kDa. The filtrate was combined with the extract, concentrated and dried at 40°C to constant weight to obtain a torreya nut extract.

[0042] The preparation method of lemon balm extract includes the following steps: taking the whole plant of lemon balm and crushing it, adding water 8 times the mass of lemon balm, then adding 0.3% hemicellulase and 0.1% pectinase by mass of lemon balm, adjusting the pH value to 4.2, enzymolysis at 40°C for 90 minutes, then adjusting the pH to 6.0, adding 0.5% bromelain by mass of lemon balm, enzymolysis at 50°C for 60 minutes, heating to 80°C for 10 minutes to inactivate the enzyme; after cooling to room temperature, adjusting the pH value to 7.0, adding 0.5% serrapeptase by mass of lemon balm, enzymolysis at 35°C for 60 minutes, heating to 80°C for 10 minutes to inactivate the enzyme; after cooling to room temperature, filtering with a 250-mesh sieve, taking the filtrate, concentrating under reduced pressure at 50°C, and drying at 50°C to constant weight to obtain the lemon balm extract.

[0043] Among them, the preparation method of the sterculia lychnophora extract includes the following steps: taking sterculia lychnophora seeds and crushing them, adding water 10 times the mass of the sterculia lychnophora seeds, and then adding 0.5% hemicellulase, 0.3% xylanase and 0.5% papain of the mass of the sterculia lychnophora seeds, and ultrasonically hydrolyzing at 20kHz for 1h under pH 5.5 and 45°C to obtain an ultrasonic hydrolyzate; the ultrasonic hydrolyzate is subjected to supercritical CO2 dynamic countercurrent extraction, and the extraction parameters are: pressure 22MPa, temperature 42°C, CO2 flow rate 12L / h, entrainer ethanol ratio 4%v / v, time 2h, to obtain an extract, and the remaining hydrolyzate is ultrafiltered using a 5kDa ultrafiltration membrane to obtain a filtrate less than 5kDa, the filtrate is combined with the extract, and concentrated and dried at 40°C to constant weight to obtain the sterculia lychnophora extract.

[0044] Among them, hemp seed, cassia seed, peach kernel, apricot kernel, Prunus mume seed, angelica, Cistanche deserticola and prune are all crushed medicinal materials into dry powder.

[0045] The method for preparing the above-mentioned plant powder for promoting intestinal peristalsis and defecation comprises the following steps: Separately sieve the extracts of Momordica grosvenori, Torreya grandiflora, lemon balm, Sterculia lychnophora, Fructus Hemp, Semen Cassiae, Peach Kernel, Apricot Kernel, Prunus Amygdalus, Semen Euphorbiae, Herba Cistanches and Prunus Parviflora through an 80-mesh sieve, take the sieve-less materials, mix them uniformly by weight to obtain a mixture, add 10wt% starch slurry which is 5% by weight of the mixture, stir uniformly, and granulate with a rocking granulator and a 30-mesh sieve to obtain granular powder, dry it at 50°C for 2h to control the moisture content of the granular powder to 4wt%, put the dried granules into the mixer again and mix for 10min to obtain a plant powder.

[0046] Example 3 A plant powder for promoting intestinal peristalsis and defecation, comprising the following raw materials in parts by mass: 5 parts of monk fruit, 6 parts of torreya grandis extract, 4 parts of lemon balm extract, 5 parts of sterculia lychnophora extract, 3 parts of hemp seed, 10 parts of cassia seed, 2 parts of peach kernel, 5 parts of apricot kernel, 2 parts of Prunus mume kernel, 5 parts of angelica sinensis, 1 part of Cistanche deserticola and 3 parts of prune.

[0047] The preparation method of the ginkgo nut extract comprises the following steps: quick-freezing the ginkgo nut kernels with liquid nitrogen for 5 minutes, crushing, and passing through an 80-mesh sieve to obtain ginkgo nut kernel powder; mixing the ginkgo nut kernel powder: water: lipase: alkaline protease in a mass ratio of 1:12:0.01:0.01, adjusting the pH value to 6.5, performing enzymatic hydrolysis at 48°C for 2.5 hours, then adjusting the pH value to 8.2, adding alkaline protease, and performing enzymatic hydrolysis at 52°C for 2.5 hours to obtain an enzymatic solution; subjecting the enzymatic solution to a supercritical CO2 dynamic countercurrent primary extraction, wherein the parameters of the primary extraction are: pressure 22MPa, temperature 48°C, C The O2 flow rate was 12 L / h and the time was 3 h to obtain a primary extract containing β-sitosterol and torreya esters; the remaining enzymatic hydrolysate was subjected to supercritical CO2 dynamic countercurrent secondary extraction, and the parameters of the secondary extraction were: pressure 32 MPa, temperature 58°C, entrainer ethanol ratio 4% v / v, flow rate 18 L / h, time 2 h to obtain a secondary extract containing torreya polyphenols and flavonoid glycosides. The primary extract and the secondary extract were combined to obtain an extract; the remaining enzymatic hydrolysate was ultrafiltered using a 5 kDa ultrafiltration membrane to obtain a filtrate less than 5 kDa. The filtrate was combined with the extract, concentrated and dried at 48°C to constant weight to obtain a torreya nut extract.

[0048] The preparation method of lemon balm extract includes the following steps: taking the whole plant of lemon balm and crushing it, adding water 8 times the mass of lemon balm, then adding 0.5% hemicellulase and 0.1% pectinase by mass of lemon balm, adjusting the pH value to 4.8, enzymatically hydrolyzing at 40°C for 150 minutes, then adjusting the pH to 6.0, adding 1% bromelain by mass of lemon balm, enzymatically hydrolyzing at 50°C for 90 minutes, heating to 80°C for 15 minutes to inactivate the enzyme; after cooling to room temperature, adjusting the pH value to 7.0, adding 1.0% serrapeptase by mass of lemon balm, enzymatically hydrolyzing at 35°C for 90 minutes, heating to 80°C for 15 minutes to inactivate the enzyme; after cooling to room temperature, filtering with a 250-mesh sieve, taking the filtrate, concentrating under reduced pressure at 58°C, and drying at 50°C to constant weight to obtain the lemon balm extract.

[0049] Among them, the preparation method of the sterculia lychnophora extract includes the following steps: taking sterculia lychnophora seeds and crushing them, adding water 12 times the mass of the sterculia lychnophora seeds, and then adding 0.5% hemicellulase, 0.5% xylanase and 0.5% papain of the mass of the sterculia lychnophora seeds, and ultrasonically hydrolyzing them at 25kHz for 1h under the conditions of pH 6.0 and 45°C to obtain ultrasonic hydrolyzate; the ultrasonic hydrolyzate is subjected to supercritical CO2 dynamic countercurrent extraction, and the extraction parameters are: pressure 28MPa, temperature 42°C, CO2 flow rate 18L / h, entrainer ethanol ratio 4%v / v, time 3h, to obtain an extract, and the remaining hydrolyzate is ultrafiltered using a 5kDa ultrafiltration membrane to obtain a filtrate less than 5kDa, the filtrate is combined with the extract, and concentrated and dried at 40°C to constant weight to obtain the sterculia lychnophora extract.

[0050] Among them, hemp seed, cassia seed, peach kernel, apricot kernel, Prunus mume seed, angelica, Cistanche deserticola and prune are all crushed medicinal materials into dry powder.

[0051] The method for preparing the above-mentioned plant powder for promoting intestinal peristalsis and defecation comprises the following steps: Separately pass the extracts of Momordica grosvenori, Torreya grandiflora, lemon balm, Sterculia lychnophora, Fructus Hemp, Fructus Cassiae, Peach Kernel, Apricot Kernel, Prunus Amygdalus, Prunus mume, Angelica sinensis, Cistanche Deserticola and Prunus nigra through a 100-mesh sieve, take the sieve-less materials, mix them uniformly according to parts by mass to obtain a mixture, add 20wt% starch slurry which is 5% by mass of the mixture, stir uniformly, and granulate with a rocking granulator and a 30-mesh sieve to obtain granular powder, dry it at 60°C for 2h to control the moisture content of the granular powder to 6wt%, put the dried granules into the mixer again and mix for 10min to obtain a plant powder.

[0052] Example 4 A plant powder for promoting intestinal peristalsis and defecation, comprising the following raw materials in parts by mass: 10 parts of Momordica grosvenori, 6 parts of Torreya grandiflora extract, 8 parts of lemon balm extract, 5 parts of Sterculia lychnophora extract, 6 parts of Cannabis sativa seeds, 10 parts of Cassia seeds, 5 parts of peach kernels, 5 parts of apricot kernels, 5 parts of Prunus mume seeds, 5 parts of Chinese angelica, 3 parts of Cistanche deserticola, and 3 parts of Prunus citriodora.

[0053] The preparation method of the torreya nut extract comprises the following steps: quick-freezing the torreya nut kernels with liquid nitrogen for 5 minutes, crushing, and passing through a 120-mesh sieve to obtain torreya nut kernel powder; mixing the powder, water, and lipase in a mass ratio of torreya nut kernel powder: water: lipase: alkaline protease = 1:12:0.015:0.01, adjusting the pH value to 7.5, performing enzymatic hydrolysis at 48°C for 3.5 hours, then adjusting the pH value to 9.0, adding alkaline protease, and performing enzymatic hydrolysis at 57°C for 2.5 hours to obtain an enzymatic solution; subjecting the enzymatic solution to supercritical CO2 dynamic countercurrent primary extraction, wherein the parameters of the primary extraction are: pressure 28MPa, temperature 48°C, The CO2 flow rate was 18 L / h and the time was 3 h to obtain a primary extract containing β-sitosterol and torreya esters; the remaining enzymatic hydrolysate was subjected to supercritical CO2 dynamic countercurrent secondary extraction, and the parameters of the secondary extraction were: pressure 38 MPa, temperature 58°C, entrainer ethanol ratio 6% v / v, flow rate 18 L / h, time 3 h to obtain a secondary extract containing torreya polyphenols and flavonoid glycosides, and the primary extract and the secondary extract were combined to obtain an extract; the remaining enzymatic hydrolysate was ultrafiltered using a 5 kDa ultrafiltration membrane to obtain a filtrate less than 5 kDa, the filtrate was combined with the extract, concentrated and dried at 48°C to constant weight to obtain a torreya nut extract.

[0054] The preparation method of lemon balm extract includes the following steps: taking the whole plant of lemon balm and crushing it, adding water 12 times the mass of lemon balm, then adding 0.5% hemicellulase and 0.3% pectinase by mass of lemon balm, adjusting the pH value to 4.8, enzymatically hydrolyzing at 50°C for 150 minutes, then adjusting the pH to 7.0, adding 1% bromelain by mass of lemon balm, enzymatically hydrolyzing at 55°C for 90 minutes, heating to 85°C for 15 minutes to inactivate the enzyme; after cooling to room temperature, adjusting the pH value to 8.0, adding 1.0% serrapeptase by mass of lemon balm, enzymatically hydrolyzing at 40°C for 90 minutes, heating to 85°C for 15 minutes to inactivate the enzyme; after cooling to room temperature, filtering with a 325-mesh sieve, taking the filtrate, concentrating under reduced pressure at 58°C, and drying at 58°C to constant weight to obtain the lemon balm extract.

[0055] Among them, the preparation method of the sterculia lychnophora extract includes the following steps: taking sterculia lychnophora seeds and crushing them, adding water 12 times the mass of the sterculia lychnophora seeds, and then adding 0.7% hemicellulase, 0.5% xylanase and 1.0% papain of the mass of the sterculia lychnophora seeds, and ultrasonically hydrolyzing at 25kHz for 2 hours at a pH of 6.0 and 55°C to obtain an ultrasonic hydrolyzate; the ultrasonic hydrolyzate is subjected to supercritical CO2 dynamic countercurrent extraction, and the extraction parameters are: pressure 28MPa, temperature 48°C, CO2 flow rate 18L / h, entrainer ethanol ratio 6%v / v, time 3h, to obtain an extract, and the remaining hydrolyzate is ultrafiltered using a 5kDa ultrafiltration membrane to obtain a filtrate less than 5kDa, the filtrate is combined with the extract, and concentrated and dried at 48°C to constant weight to obtain the sterculia lychnophora extract.

[0056] Among them, hemp seed, cassia seed, peach kernel, apricot kernel, Prunus mume seed, angelica, Cistanche deserticola and prune are all crushed medicinal materials into dry powder.

[0057] The method for preparing the above-mentioned plant powder for promoting intestinal peristalsis and defecation comprises the following steps: Separately pass the extracts of Momordica grosvenori, Torreya grandiflora, lemon balm, Sterculia lychnophora, Fructus Hemp, Semen Cassiae, Peach Kernel, Apricot Kernel, Prunus Amygdalus, Rhizoma Cistanches, and Prunus Parviflora through a 100-mesh sieve, take the sieve-less materials, mix them uniformly by weight to obtain a mixture, add 20wt% starch slurry which is 10% by weight of the mixture, stir uniformly, and granulate with a rocking granulator and a 35-mesh sieve to obtain granular powder, dry it at 60°C for 4h to control the moisture content of the granular powder to 6wt%, put the dried granules into the mixer again and mix for 15min to obtain a plant powder.

[0058] Example 5 A plant powder for promoting intestinal peristalsis and defecation, comprising the following raw materials in parts by mass: 10 parts of monk fruit, 3 parts of torreya grandis extract, 8 parts of lemon balm extract, 2 parts of sterculia lychnophora extract, 6 parts of hemp seed, 5 parts of cassia seed, 5 parts of peach kernel, 2 parts of apricot kernel, 5 parts of Prunus mume seed, 2 parts of angelica sinensis, 3 parts of Cistanche deserticola and 1 part of prune.

[0059] The preparation method of the ginkgo nut extract comprises the following steps: quick-freezing the ginkgo nut kernels with liquid nitrogen for 3 minutes, crushing, and passing through a 120-mesh sieve to obtain ginkgo nut kernel powder; mixing the ginkgo nut kernel powder: water: lipase: alkaline protease in a mass ratio of 1:10:0.015:0.006, adjusting the pH value to 7.5, performing enzymatic hydrolysis at 42°C for 3.5 hours, then adjusting the pH value to 8.8, adding alkaline protease, and performing enzymatic hydrolysis at 57°C for 1.5 hours to obtain an enzymatic solution; subjecting the enzymatic solution to a supercritical CO2 dynamic countercurrent primary extraction, wherein the parameters of the primary extraction are: pressure 28 MPa, temperature 42°C, The CO2 flow rate was 18 L / h and the time was 2 h to obtain a primary extract containing β-sitosterol and torreya esters; the remaining enzymatic hydrolysate was subjected to supercritical CO2 dynamic countercurrent secondary extraction, and the parameters of the secondary extraction were: pressure 38 MPa, temperature 52°C, entrainer ethanol ratio 6% v / v, flow rate 12 L / h, time 3 h to obtain a secondary extract containing torreya polyphenols and flavonoid glycosides. The primary extract and the secondary extract were combined to obtain an extract; the remaining enzymatic hydrolysate was ultrafiltered using a 5 kDa ultrafiltration membrane to obtain a filtrate less than 5 kDa. The filtrate was combined with the extract, concentrated and dried at 40°C to constant weight to obtain a torreya nut extract.

[0060] The preparation method of lemon balm extract includes the following steps: taking the whole plant of lemon balm and crushing it, adding water 12 times the mass of lemon balm, then adding 0.3% hemicellulase and 0.3% pectinase by mass of lemon balm, adjusting the pH value to 4.2, enzymolysis at 50°C for 90 minutes, then adjusting the pH to 7.0, adding 0.5% bromelain by mass of lemon balm, enzymolysis at 55°C for 60 minutes, heating to 85°C for 10 minutes to inactivate the enzyme; after cooling to room temperature, adjusting the pH value to 8.0, adding 0.5% serrapeptase by mass of lemon balm, enzymolysis at 40°C for 60 minutes, heating to 85°C for 10 minutes to inactivate the enzyme; after cooling to room temperature, filtering with a 325-mesh sieve, taking the filtrate, concentrating under reduced pressure at 50°C, and drying at 58°C to constant weight to obtain the lemon balm extract.

[0061] Among them, the preparation method of the sterculia lychnophora extract includes the following steps: taking sterculia lychnophora seeds and crushing them, adding water 10 times the mass of the sterculia lychnophora seeds, and then adding 0.7% hemicellulase, 0.3% xylanase and 1.0% papain of the mass of the sterculia lychnophora seeds, and ultrasonically hydrolyzing at 20kHz for 2 hours at a pH of 5.5 and 55°C to obtain an ultrasonic hydrolyzate; the ultrasonic hydrolyzate is subjected to supercritical CO2 dynamic countercurrent extraction, and the extraction parameters are: pressure 22MPa, temperature 48°C, CO2 flow rate 12L / h, entrainer ethanol ratio 6%v / v, time 2h, to obtain an extract, and the remaining hydrolyzate is ultrafiltered using a 5kDa ultrafiltration membrane to obtain a filtrate less than 5kDa, the filtrate is combined with the extract, and concentrated and dried at 48°C to constant weight to obtain the sterculia lychnophora extract.

[0062] Among them, hemp seed, cassia seed, peach kernel, apricot kernel, Prunus mume seed, angelica, Cistanche deserticola and prune are all crushed medicinal materials into dry powder.

[0063] The method for preparing the above-mentioned plant powder for promoting intestinal peristalsis and defecation comprises the following steps: Separately sieve the extracts of Momordica grosvenori, Torreya grandiflora, lemon balm, Sterculia lychnophora, Fructus Hemp, Fructus Cassiae, Peach Kernel, Apricot Kernel, Prunus Amygdalus, Fructus Euphrasiae, Angelica Sinensis, Cistanche Deserticola and Prunus Western Prunus through an 80-mesh sieve, take the sieve-less materials, mix them uniformly according to parts by mass to obtain a mixture, add 10% by mass of a starch slurry with a concentration of 10 wt % to the mixture, stir uniformly, and granulate with a rocking granulator and a 35-mesh sieve to obtain granular powder, dry it at 50° C. for 4 h to control the moisture content of the granular powder to 4 wt %, put the dried granules into the mixer again and mix for 15 min to obtain a plant powder.

[0064] In the above embodiments: the enzymatic activity of cellulase is 20,000 U / g; the enzymatic activity of pectinase is 20,000 U / g; the enzymatic activity of β-glucosidase is 100 U / g; the enzymatic activity of papain is 200,000 U / g; the enzymatic activity of lipase is 100,000 U / g; the alkaline protease is Bacillus licheniformis protease, with an enzymatic activity of 200,000 U / g; the enzymatic activity of hemicellulase is 100,000 U / g; the enzymatic activity of bromelain is 200,000 U / g; the enzymatic activity of serrapeptase is 200,000 U / g; the enzymatic activity of xylanase is 10,000 U / g; the bacterial activity of Lactobacillus plantarum N13 is 10 billion U / g; and the bacterial activity of Candida utilis is 10 billion U / g.

[0065] Comparative Example 1 In the plant powder, the mass fraction of Momordica grosvenori is 2 parts, and the mass fraction of Torreya grandis extract is 8 parts; other parameters and methods are the same as those in Example 1.

[0066] Comparative Example 2 In the plant powder, the mass fraction of lemon balm extract is 1 part, and the mass fraction of sterculia lychnophora extract is 8 parts; other parameters and methods are the same as those in Example 1.

[0067] Comparative Example 3 In the preparation method of the Torreya grandis extract, alkaline protease is not used for enzymatic hydrolysis, and the remaining enzymatic hydrolyzate is not ultrafiltered using a 5 kDa ultrafiltration membrane (the remaining enzymatic hydrolyzate is discarded); other parameters and methods are the same as in Example 1.

[0068] Comparative Example 4 In the preparation method of the Torreya grandis extract, supercritical CO2 dynamic countercurrent secondary extraction is not performed; other parameters and methods are the same as in Example 1.

[0069] Comparative Example 5 In the preparation method of lemon balm extract, bromelain is not used for enzymatic hydrolysis; other parameters and methods are the same as those in Example 1.

[0070] Comparative Example 6 In the preparation method of lemon balm extract, serrapeptase is not used for enzymatic hydrolysis; other parameters and methods are the same as those in Example 1.

[0071] Comparative Example 7 In the preparation method of the Sterculia lychnophora extract, papain is not used for enzymatic hydrolysis, and the remaining enzymatic hydrolysate is not ultrafiltered using a 5kDa ultrafiltration membrane (the remaining enzymatic hydrolysate is discarded); other parameters and methods are the same as in Example 1.

[0072] The plant powders prepared in the above embodiments and comparative examples were tested for safety and efficacy.

[0073] 1. Safety testing: 130 healthy adult Kunming mice weighing 18g to 22g, half male and half female, were randomly divided into 13 groups (10 mice per group). One group served as a blank control group and was gavage-administered with normal saline. The remaining Example and Comparative Example groups were gavage-administered with 10g / kg body weight of the corresponding plant powder. The mice were then observed for 14 days, with symptoms of poisoning and deaths recorded. The mice's general condition, including appearance, behavior, diet, and weight changes, was also observed. The results showed that no poisoning or mortality occurred in any of the groups, demonstrating that the product ingredients are safe and non-toxic.

[0074] 2. Efficacy testing: Small Intestinal Propulsion Test: 130 healthy adult Kunming mice weighing 20-25g (half male and half female) were randomly divided into 13 groups (10 mice per group), one of which served as a blank control group. After fasting for 24 hours, each Example and Comparative Example group was administered 2 ml of the corresponding plant powder solution (concentration: 0.2 g / mL). The blank control group received an equal volume of normal saline. This treatment was repeated for three consecutive days. On the third day, 30 minutes after administration, each mouse was gavage-administered 0.5 ml of a 5% charcoal-containing gum arabic solution. Twenty minutes later, five mice in each group were sacrificed, and the small intestines were quickly removed. The length of charcoal protrusion and the total length of the small intestine were measured, and the small intestinal propulsion rate was calculated (small intestinal propulsion rate = charcoal protrusion length / total small intestine length × 100%). The time to first melena was observed for the remaining five mice. The test results are shown in Table 1 below.

[0075] 3. Patient evaluation: Ten patients with long-term constipation (half male and half female) were selected and took the plant powder prepared in Example 1, 1.5 g per day; after 7 days, the constipation of all 10 patients was significantly improved, and bowel movements were smooth; no constipation occurred after taking it for 14 consecutive days, and no recurrence occurred after stopping the drug for 30 days.

[0076] Table 1 Results of the mouse small intestinal propulsion rate and defecation time test (average values) Sample Small intestinal propulsion rate (%) Defecation time (min) Blank control group 38.4 106 Example 1 67.2 71 Example 2 64.7 75 Example 3 65.9 73 Example 4 68.5 69 Example 5 65.3 74 Comparative Example 1 60.1 83 Comparative Example 2 61.3 81 Comparative Example 3 59.4 85 Comparative Example 4 61.0 82 Comparative Example 5 59.7 83 Comparative Example 6 60.2 84 Comparative Example 7 61.5 79 From the above experimental results, it can be seen that the plant powders prepared in Examples 1 to 5 have safe ingredients and no toxic reactions, can effectively stimulate intestinal peristalsis, reduce defecation time, and obviously play the role of moisturizing the intestines and promoting bowel movements, and improving constipation symptoms.

[0077] From the results of Comparative Example 1, it can be seen that after enzymatic hydrolysis and fermentation, the characteristic small molecule peptides, fermentation products, short-chain fatty acids (SCFAs) and the like contained in the extract of Momordica grosvenori have a good effect on promoting intestinal peristalsis. Among them, the short-chain fatty acids (SCFAs) can stimulate intestinal epithelial cells to secrete 5-hydroxytryptamine. 5-hydroxytryptamine, as a neurotransmitter, can effectively stimulate the contraction of intestinal smooth muscle, thereby accelerating intestinal peristalsis. The extract of Torreya grandis contains rich characteristic small molecule peptides, fatty oils, and contains polysaccharides, volatile oils and other substances, which can indirectly play a role in lubricating the intestine and promoting intestinal peristalsis. However, the ratio of the two ingredients needs to be precisely adjusted to achieve the optimal synergistic effect. Comparative Example 1 reduces the content of Momordica grosvenori and increases the content of Torreya grandis extract. The ratio is not appropriate, affecting the synergistic effect, and the synergistic effect of the plant powder promoting intestinal peristalsis is weakened.

[0078] The results of Comparative Example 2 show that the volatile oil and other components contained in the lemon balm extract can regulate the intestinal environment, and the Sterculia lychnophora extract has the effect of increasing intestinal moisture and promoting intestinal peristalsis. The two ingredients have a better synergistic effect when used in a specific ratio range. The ratio of lemon balm extract and Sterculia lychnophora extract in Comparative Example 2 is changed, which does not achieve the optimal synergistic effect, and the synergistic effect of the product in moistening the intestine and promoting laxative effect is weakened.

[0079] From the results of Comparative Examples 3 to 4, it can be seen that in the preparation method of the Torreya grandis extract, alkaline protease is not used for enzymatic hydrolysis and the remaining enzymatic hydrolysis liquid is discarded, or supercritical CO2 dynamic countercurrent secondary extraction is not performed, the effective substance components of the Torreya grandis extract obtained by extraction are changed, and some effective substance components are missing, and the product's laxative effect is weakened.

[0080] From the results of Comparative Examples 5 and 6, it can be seen that in the preparation method of the lemon balm extract, if bromelain is not used for enzymatic hydrolysis, or serrapeptase is not used for enzymatic hydrolysis, the effective substance composition of the lemon balm extract obtained by extraction changes, and the laxative effect of the product is weakened.

[0081] From the results of Comparative Example 7, it can be seen that in the preparation method of the Sterculia lychnophora extract, papain is not used for enzymatic hydrolysis and the remaining enzymatic hydrolysis liquid is discarded. The effective ingredients of the Sterculia lychnophora extract obtained by extraction are changed, and some effective ingredients are missing, and the laxative effect of the product is weakened.

Claims

1. A plant powder for promoting intestinal peristalsis and defecation, characterized in that: The plant powder comprises the following raw materials in parts by weight: 5 to 10 parts of monk fruit, 3 to 6 parts of torreya grandis extract, 4 to 8 parts of lemon balm extract, 2 to 5 parts of sterculia lychnophora extract, 3 to 6 parts of hemp seed, 5 to 10 parts of cassia seed, 2 to 5 parts of peach kernel, 2 to 5 parts of apricot kernel, 2 to 5 parts of prunus mume seed, 2 to 5 parts of angelica sinensis, 1 to 3 parts of cistanche deserticola and 1 to 3 parts of prune; The Torreya grandis extract comprises a hydrolyzate obtained by sequentially hydrolyzing Torreya grandis kernels with lipase and alkaline protease, a product obtained by twice extracting the hydrolyzate with supercritical CO2 dynamic countercurrent, and a product below 5 kDa obtained by ultrafiltration of the remaining hydrolyzate with an ultrafiltration membrane; Lemon balm extract comprises a product obtained by hydrolyzing lemon balm with hemicellulase and pectinase, followed by sequential hydrolysis with bromelain and serrapeptase; The sterculia lychnophora extract comprises an enzymatic hydrolyzate obtained by ultrasonic enzymolysis of sterculia lychnophora seeds with hemicellulase, xylanase and papain, a product obtained by supercritical CO2 dynamic countercurrent extraction of the enzymatic hydrolyzate, and a product below 5 kDa obtained by ultrafiltration of the remaining enzymatic hydrolyzate with an ultrafiltration membrane.

2. The plant powder for promoting intestinal peristalsis and defecation according to claim 1, characterized in that: The preparation of the Chinese torreya nut extract includes: quick-freezing the Chinese torreya nut kernels with liquid nitrogen, crushing them to obtain Chinese torreya nut kernel powder; mixing the powder, water and lipase in a mass ratio of Chinese torreya nut kernel powder: water: lipase: alkaline protease = 1: (10-12): (0.01-0.015): (0.006-0.01), adjusting the pH, performing enzymatic hydrolysis, then adjusting the pH and adding alkaline protease, performing enzymatic hydrolysis to obtain an enzymatic hydrolyzate; subjecting the enzymatic hydrolyzate to a primary extraction with supercritical CO2 dynamic countercurrent to obtain a primary extract; subjecting the remaining enzymatic hydrolyzate to a secondary extraction with supercritical CO2 dynamic countercurrent to obtain a secondary extract, combining the primary extract and the secondary extract to obtain an extract; ultrafiltration of the remaining enzymatic hydrolyzate using an ultrafiltration membrane to obtain a filtrate, combining the filtrate and the extract, concentrating and drying to obtain the Chinese torreya nut extract.

3. The plant powder for promoting intestinal peristalsis and defecation according to claim 2, characterized in that: The time for the liquid nitrogen quick freezing is 3 minutes to 5 minutes; the particle size of the crushing is through an 80-mesh to 120-mesh sieve; the parameters of the supercritical CO2 dynamic countercurrent primary extraction are: pressure 22MPa to 28MPa, temperature 42°C to 48°C, CO2 flow rate 12L / h to 18L / h, time 2h to 3h; the parameters of the supercritical CO2 dynamic countercurrent secondary extraction are: pressure 32MPa to 38MPa, temperature 52°C to 58°C, entrainer ethanol ratio 4%v / v to 6%v / v, flow rate 12L / h to 18L / h, time 2h to 3h.

4. The plant powder for promoting intestinal peristalsis and defecation according to claim 1, characterized in that: The preparation of lemon balm extract includes: crushing lemon balm, adding water, then adding 0.3% to 0.5% of hemicellulase and 0.1% to 0.3% of pectinase by weight of the lemon balm, adjusting the pH value for enzymatic hydrolysis, then adjusting the pH value, adding 0.5% to 1% of bromelain by weight of the lemon balm, enzymatic hydrolysis, and heating to inactivate the enzyme; after cooling, adjusting the pH value, adding 0.5% to 1.0% of serrapeptase by weight of the lemon balm, enzymatic hydrolysis, and heating to inactivate the enzyme; after cooling, filtering, concentrating and drying the filtrate to obtain the lemon balm extract.

5. The plant powder for promoting intestinal peristalsis and defecation according to claim 4, characterized in that: The temperature for heating the enzyme to be inactivated is 80°C to 85°C, and the time for heating the enzyme to be inactivated is 10 minutes to 15 minutes. The filtration is performed using a mesh filtration, and the mesh size of the mesh is 250 to 325 meshes. The concentration is performed using a low-temperature reduced-pressure concentration, and the concentration temperature is below 60°C. The drying is performed using a low-temperature drying, and the drying temperature is below 60°C.

6. The plant powder for promoting intestinal peristalsis and defecation according to claim 1, characterized in that: The preparation of the Sterculia lychnophora extract includes: crushing Sterculia lychnophora seeds, adding water, then adding 0.5% to 0.7% of hemicellulase, 0.3% to 0.5% of xylanase and 0.5% to 1.0% of papain based on the weight of the Sterculia lychnophora seeds, ultrasonic enzymolysis to obtain ultrasonic enzymatic hydrolysate, supercritical CO2 dynamic countercurrent extraction to obtain an extract; ultrafiltration of the remaining enzymatic hydrolysate through an ultrafiltration membrane to obtain a filtrate, combining the filtrate and the extract, concentrating and drying to obtain the Sterculia lychnophora extract.

7. The plant powder for promoting intestinal peristalsis and defecation according to claim 6, characterized in that: The frequency of the ultrasound is 20kHz~25kHz, and the time of the ultrasound is 1h~2h; the extraction parameters are: pressure 22MPa~28MPa, temperature 42℃~48℃, CO2 flow rate 12L / h~18L / h, entrainer ethanol ratio 4%v / v~6%v / v, time 2h~3h.

8. The plant powder for promoting intestinal peristalsis and defecation according to claim 1, characterized in that: The Momordica grosvenori, hemp seed, cassia seed, peach kernel, apricot kernel, Prunus mume seed, Chinese angelica, Cistanche deserticola and prune are all ground into dry powder.

9. A method for preparing a plant powder for promoting intestinal peristalsis and defecation, for preparing the plant powder for promoting intestinal peristalsis and defecation according to any one of claims 1 to 8, characterized in that: The preparation method comprises the following steps: Separately sieve the following: Momordica grosvenori, Torreya grandiflora extract, lemon balm extract, Sterculia lychnophora extract, Cannabis sativa seed, Cassia seed, peach kernel, apricot kernel, Prunus armeniaca seed, Angelica sinensis, Cistanche deserticola and Prunus citriodora, take the sieve underflow, mix them uniformly by weight to obtain a mixture, add 5% to 10% by weight of starch slurry of the mixture, stir uniformly, and granulate with a rocking granulator and sieve with a 30-35 mesh sieve to obtain granular powder, dry it to control the moisture content of the granular powder to 4wt% to 6wt%, put the dried granules into the mixer again, and mix them for 10min to 15min to obtain a plant powder.

10. The method for preparing a plant powder for promoting intestinal peristalsis and defecation according to claim 9, characterized in that: The starch concentration of the starch slurry is 10 wt% to 20 wt%; the drying temperature is 50° C. to 60° C.; and the drying time is 2 h to 4 h.