A device for concentrating and purifying an antibacterial peptide

Abstract

The application discloses a kind of concentrated purification device of antibacterial peptide, more particularly to concentrated purification device technical field, including sewage tank and liquid storage tank, the sewage tank upside is fixed with filter part, one side of the liquid storage tank is fixed with water storage tank, the liquid storage tank upside is fixed with water pump one, the water storage tank upside is fixed with concentration part, the concentration part one side is equipped with purification assembly.The concentrated purification device of antibacterial peptide described in the application can carry out tangential filtration to broken cell liquid by the filter part, avoid the deposition of macromolecular substances such as cell debris and lipids on the surface of the membrane, and the multiple filter assemblies can also be back-flushed, thereby reducing the cell debris attached to the surface of the membrane in the filter assembly, avoiding the problem of frequent replacement due to clogging of the filter membrane, and at the same time, the impurities in the filtered supernatant are less, reducing the impact on product quality.

Description

Technical Field

[0001] This invention relates to the field of concentration and purification equipment, and more particularly to a concentration and purification equipment for antimicrobial peptides. Background Technology

[0002] Antimicrobial peptides are small molecule amino acid polymers produced based on biotechnology. They have significant effects on viral diseases. Currently, some antimicrobial peptide technologies have entered clinical trials. In the near future, antimicrobial peptide drugs are expected to be widely used in the treatment of malignant diseases.

[0003] During the concentration and purification of antimicrobial peptides, the filter membrane is easily clogged by cell debris, and frequent replacement increases the operating cost. If it is not cleaned, the filtered antimicrobial peptide filtrate will have too many impurities, affecting the quality of the product. In addition, during the concentration process, the flux of the ultrafiltration membrane decreases due to the adsorption of impurities on the membrane surface, requiring frequent cleaning. Furthermore, the solute concentration gradient on the membrane surface further aggravates membrane fouling and shortens the membrane's lifespan. Summary of the Invention

[0004] The purpose of this invention is to address the problems encountered during the concentration and purification of antimicrobial peptides, such as the clogging of deep filtration membranes by cell debris, which increases operating costs due to frequent replacements, excessive impurities in the filtered antimicrobial peptide filtrate due to uncleanliness affecting product quality, and the reduced flux of ultrafiltration membranes due to impurity adsorption during concentration, requiring frequent cleaning. Therefore, this invention proposes an antimicrobial peptide concentration and purification device.

[0005] To achieve the above objectives, the present invention employs the following technology: a device for concentrating and purifying antimicrobial peptides.

[0006] It includes a wastewater tank and a storage tank. A filter component is fixed on the upper side of the wastewater tank, a water storage tank is fixed on one side of the storage tank, a water pump is fixed on the upper side of the storage tank, a concentration component is fixed on the upper side of the water storage tank, and a purification component is installed on one side of the concentration component.

[0007] The filter component includes a filter box and a water pump two fixedly connected to the filter box. The lower side of the filter box is connected to the inner cavity of the liquid storage tank through multiple delivery pipes. Multiple evenly distributed fixing blocks are fixed in the inner cavity of the filter box. Filter components are embedded inside each of the multiple fixing blocks. Two adjacent filter components are connected by a connecting pipe. Two filter components located on both sides are respectively fixedly connected to a three-way pipe one and a three-way pipe two.

[0008] A tangential filtration flow loop is formed between multiple filter components through a three-way pipe and a connecting pipe. Clean water is drawn from the water storage tank by a second water pump, which can backwash the multiple filter components.

[0009] As a kind of antibacterial peptide of the above technical concentrated purification device further described:

[0010] The vertical part of the three-way pipe is connected with electric control valve one, one side of the electric control valve one is communicated with the inner cavity of sewage tank through conveying pipe, the horizontal part of the three-way pipe two is fixed with electric control valve two away from the side of filter assembly, one side of the electric control valve two is communicated with the inner cavity of sewage tank through conveying pipe, the vertical part of the three-way pipe two is connected with the output end of water pump two through conveying pipe, the input end of water pump two is communicated with the inner cavity of water storage tank through conveying pipe.

[0011] As a kind of antibacterial peptide of the above technical concentrated purification device further described:

[0012] The side of the filter box is fixed with driving motor, the output end of the driving motor is fixed with connecting shaft, the outer surface of the connecting shaft is fixed with a plurality of winding rollers matched with filter assembly, the outer surface of a plurality of winding rollers is fixed with two groups of connecting ropes.

[0013] As a kind of antibacterial peptide of the above technical concentrated purification device further described:

[0014] The filter assembly comprises fixed ring, the inner surface of the fixed ring is provided with two symmetrical filter membranes and two symmetrical filter screens, two filter screens are located inside two filter membranes, two filter screens are fixedly connected with connecting shaft, the inner surface of the fixed ring is fixed with two groups of symmetrical brush strips, the brush strips in the same group are close to the surface of filter screen on the same side.

[0015] As a kind of antibacterial peptide of the above technical concentrated purification device further described:

[0016] The inner surface of the fixed ring is fixed with two symmetrical mounting plates through supporting spring, two mounting plates are fixedly connected with two groups of connecting ropes close to each other respectively, two mounting plates are slidably connected with two symmetrical moving plates on the side close to each other, the inner surface of the fixed ring is provided with sliding groove matched with moving plate, one side of two moving plates on the same side is fixed with fixed plate, the side close to each other of two fixed plates is fixed with connecting spring, two connecting springs are fixedly connected with moving plate.

[0017] As a kind of antibacterial peptide of the above technical concentrated purification device further described:

[0018] The concentration component includes a concentration tank fixed on the upper side of the water storage tank, a water pump three fixed on the upper side of the concentration tank, a concentration pipe fixed in the inner cavity of the concentration tank, an ultrafiltration membrane assembly fixed in the inner cavity of the concentration pipe, an input pipe and an output pipe fixed on the two sides of the ultrafiltration membrane assembly respectively, a spiral flow channel fixed between the input pipe and the output pipe and located in the inner part of the ultrafiltration membrane assembly, and a water pump one with an output end communicated with the inner cavity of the input pipe through a conveying pipe and an input end communicated with the inner cavity of the liquid storage tank through a conveying pipe.

[0019] Further description of the concentration and purification device for the antibacterial peptide according to the above-mentioned technology:

[0020] The outer surface of the concentration pipe is fixed with a sewage and drainage pipe communicated with the inner cavity of the concentration pipe near the side of the input pipe, one end of the sewage and drainage pipe away from the input pipe is fixed with a three-way electric control valve, the horizontal part of the three-way electric control valve is communicated with the inner cavity of the water storage tank through a conveying pipe on one side, and the horizontal part of the three-way electric control valve is communicated with the inner cavity of the sewage tank through a conveying pipe on the other side.

[0021] Further description of the concentration and purification device for the antibacterial peptide according to the above-mentioned technology:

[0022] The outer surface of the concentration pipe is fixed with a water inlet pipe communicated with the inner cavity of the concentration pipe near the side of the output pipe, the water inlet pipe is connected with the output end of the water pump three, and the input end of the water pump three is communicated with the inner cavity of the water storage tank through a conveying pipe.

[0023] According to the concentration and purification device for the antibacterial peptide according to the above-mentioned technology, the beneficial effects of the present application are as follows:

[0024] 1. The filter component can perform tangential filtration on the broken cell liquid, avoid the deposition of cell fragments, lipids and other macromolecular substances on the surface of the membrane, and perform backwashing on multiple filter assemblies, so as to reduce the cell fragments attached to the surface of the membrane in the filter assembly, avoid the problem of frequent replacement due to the clogging of the filter membrane, and reduce the impurities in the filtered supernatant, thereby reducing the influence on the product quality; the broken cell liquid flows from the three-way pipe one to the inner cavity of the filter assembly, and then flows to the next filter assembly through the connecting pipe, so as to form a tangential filtration system; the water pump two extracts the clean water in the inner cavity of the water storage tank and conveys the clean water to the three-way pipe two, at this time, the electric control valve two is closed, the clean water in the three-way pipe two is conveyed to the tangential filtration system composed of multiple filter assemblies and connecting pipes, and the flow path of the broken cell liquid is opposite, so as to form backwashing.

[0025] 2. The device can clean the filter screen by setting the filter assembly and the cooperation between the winding roller and the connecting rope, remove the impurities on the filter screen and filter membrane, and ensure the flux of the filter assembly. During backwashing, first, the connecting shaft is driven to rotate forward, so that the filter screen rotates. Since the brush bar is fixed, the brush bar can clean the surface of the filter screen and loosen the cell fragments on the filter screen. Then the connecting shaft is driven to rotate reversely, at this time the filter screen no longer rotates, the winding roller rotates, and the two mounting plates in the filter assembly move towards each other through the two groups of connecting ropes, so that the liquid flow area between the two filter assemblies is reduced, the liquid flow speed is improved, and the impurities on the filter screen and filter membrane can be further washed away, and the backwashing effect is further improved.

[0026] 3. The device can form turbulent flow in the spiral flow channel by setting the concentration component, destroy the static boundary layer on the membrane surface, reduce the local concentration accumulation of the antibacterial peptide on the membrane surface, reduce the concentration polarization, avoid the decrease of the filtration efficiency caused by the concentration gradient, and also can backwash the ultrafiltration membrane assembly to ensure the flux of the ultrafiltration membrane assembly. The fixed block delivers the antibacterial peptide supernatant in the inner cavity of the liquid storage tank to the inner cavity of the input pipe, then forms turbulent flow through the acceleration of the spiral flow channel, then is ultrafiltered through the ultrafiltration membrane assembly, so that water flows to the sewage and drainage pipe side, then is discharged to the inner cavity of the water storage tank through the sewage and drainage pipe, and the antibacterial peptide concentrate is delivered from the output pipe to the purification assembly for purification. The water pump three extracts the clean water in the inner cavity of the water storage tank to the water inlet pipe, delivers it to the inner cavity of the concentration pipe through the three-way pipe two, and backwashes the ultrafiltration membrane assembly. The waste liquid of backwashing flows to the inner cavity of the sewage tank through the sewage and drainage pipe and the three-way electric valve. The backwashing of the ultrafiltration membrane assembly can restore the flux of the ultrafiltration membrane assembly and improve the filtration efficiency. BRIEF DESCRIPTION OF DRAWINGS

[0027] Figure 1 The overall structure schematic diagram provided by the embodiment of the application is shown Figure 1 ;

[0028] Figure 2 The overall structure schematic diagram provided by the embodiment of the application is shown Figure 2 ;

[0029] Figure 3 The filter component part structure schematic diagram provided by the embodiment of the application is shown Figure 1 ;

[0030] Figure 4 The filter component part structure schematic diagram provided by the embodiment of the application is shown Figure 2 ;

[0031] Figure 5 The filter component structure sectional view provided by the embodiment of the application is shown

[0032] Figure 6 A tangential filtration structure schematic diagram of a plurality of filter assemblies is shown according to an embodiment of the present application;

[0033] Figure 7 A filter assembly structure sectional view is shown according to an embodiment of the present application;

[0034] Figure 8 A filter assembly structure exploded view is shown according to an embodiment of the present application;

[0035] Figure 9 A filter assembly partial structure schematic diagram is shown according to an embodiment of the present application;

[0036] Figure 10 A cell liquid flow tangential filtration schematic diagram is shown according to an embodiment of the present application;

[0037] Figure 11 An antibacterial peptide supernatant flow schematic diagram is shown according to an embodiment of the present application;

[0038] Figure 12 A backwash water flow schematic diagram in a filter component is shown according to an embodiment of the present application;

[0039] Figure 13 A concentration component partial structure schematic diagram is shown according to an embodiment of the present application;

[0040] Figure 14 A concentration component structure sectional view is shown according to an embodiment of the present application;

[0041] Figure 15 A concentration tube and ultrafiltration membrane assembly structure sectional view is shown according to an embodiment of the present application;

[0042] Figure 16 An antibacterial peptide supernatant and precipitated clean water flow schematic diagram is shown according to an embodiment of the present application;

[0043] Figure 17 An ultrafiltration membrane assembly backwash clean water flow schematic diagram is shown according to an embodiment of the present application;

[0044] Figure 18 A concentration component cooperating with a sewage tank schematic diagram is shown according to an embodiment of the present application.

[0045] Legend:

[0046] 10, sewage tank; 11, liquid storage tank; 12, water storage tank; 13, water pump I;

[0047] 20, filter component; 21, filter box; 22, water pump two; 23, fixed block; 24, drive motor; 241, connecting shaft; 242, winding roller; 243, connecting rope; 25, tee pipe one; 26, electric control valve one;

[0048] 27, filter assembly; 271, fixed ring; 272, filter membrane; 273, filter screen; 274, mounting plate; 275, moving plate; 276, brush strip; 277, connecting spring; 278, fixed plate;

[0049] 28, tee pipe two; 29, electric control valve two;

[0050] 30, concentration component; 31, concentration box; 32, water pump three; 33, input pipe; 34, concentration pipe; 35, ultrafiltration membrane assembly; 36, spiral flow channel; 37, output pipe; 38, water inlet pipe; 39, sewage discharge pipe; 391, tee electric control valve;

[0051] 40, purification assembly. DETAILED DESCRIPTION

[0052] The technical antibacterial peptide concentration and purification device in the embodiments of the present application will be described clearly and completely below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0053] Embodiment one

[0054] As shown in Figure 1 and Figure 2 , an antibacterial peptide concentration and purification device includes a sewage tank 10 and a liquid storage tank 11. The sewage tank 10 is used to hold impurities after filtration and liquid for backwashing. The liquid storage tank 11 is used to store filtered cell liquid. The sewage tank 10 has a filter component 20 fixed on the upper side. The filter component 20 is used to filter broken cell liquid. The liquid storage tank 11 has a water storage tank 12 fixed on one side. The water storage tank 12 is used to store clean water for backwashing and ultrafiltrated clean water. The liquid storage tank 11 has a water pump one 13 fixed on the upper side. The water storage tank 12 has a concentration component 30 fixed on the upper side. The water pump one 13 is used to extract filtered cell liquid in the inner cavity of the liquid storage tank 11 to the inner cavity of the concentration component 30 for ultrafiltration. The concentration component 30 has a purification assembly 40 installed on one side.

[0055] The purification assembly 40 comprises a chromatography system, both of which are prior art, for purifying the antibacterial peptide concentrate. First, ion exchange chromatography (IEX) is used to adsorb the target peptide in a low-salt buffer based on the cationic properties of the antibacterial peptide, and then the salt concentration (such as NaCl) is increased in a gradient to selectively elute and remove negatively charged impurities. Then, gel filtration chromatography (GFC) is used to separate based on molecular weight differences, desalt or remove large molecular aggregates. Finally, high-resolution purification is achieved by reverse-phase high-performance liquid chromatography (RP-HPLC) on a hydrophobic C18 / C8 column with an acetonitrile / water gradient elution, and the target peak is collected by ultraviolet detection (214-280 nm). The whole process takes into account the differences in charge, molecular size and hydrophobicity, and finally obtains high-purity antibacterial peptides, while the exposure time of organic solvents needs to be controlled to maintain their biological activity.

[0056] Further, as shown in Figures 3-6 The filter assembly 20 comprises a filter box 21 and a water pump 22 fixedly connected with the filter box 21. The filter box 21 is fixed on the upper side of the sewage tank 10, and the water pump 22 is fixed on the upper side of the filter box 21. The lower side of the filter box 21 is communicated with the inner cavity of the liquid storage tank 11 through a plurality of conveying pipes. The liquid in the inner cavity of the filter box 21 can flow into the inner cavity of the liquid storage tank 11 for storage. A plurality of fixed blocks 23 are fixedly arranged in the inner cavity of the filter box 21. The interval distance between adjacent two fixed blocks 23 is the same. A plurality of filter assemblies 27 are embedded in the inner part of the fixed blocks 23. The filter assemblies 27 are fixedly arranged in the inner part of the fixed blocks 23 and are used for filtering the broken cell liquid,

[0057] The adjacent two filter assemblies 27 are connected through a connecting pipe. The adjacent two connecting pipes are arranged in an up-down distribution mode, so that a tangential flow loop is formed between the plurality of filter assemblies 27. The two filter assemblies 27 located on the two sides are fixedly connected with a three-way pipe 25 and a three-way pipe 28 respectively. The three-way pipe 25 is used for connecting the disc centrifuge to convey the broken cell liquid into the inner cavity of the three-way pipe 25. A one-way valve is arranged between the disc centrifuge and the three-way pipe 25 to prevent the liquid from flowing back.

[0058] As shown in Figure 5 and Figure 6 A tangential filtration flow loop is formed between the plurality of filter assemblies 27 through the three-way pipe 25 and the connecting pipe. The circulation mode can reduce the membrane blockage. The water pump 22 can backwash the plurality of filter assemblies 27, so as to reduce the frequency of replacing the membrane, prolong the service life of the membrane, and the tangential flow liquid flows parallel to the membrane surface to generate high shear force to flush the membrane surface and reduce the deposition of macromolecular substances such as cell fragments and lipids on the membrane surface.

[0059] Then, the vertical part of the three-way pipe 25 is connected with the electric control valve 26, which is a kind of valve that can be controlled by electricity to open or close. The electric control valve 26 is connected with the inner cavity of the sewage tank 10 through a conveying pipe on one side. The liquid for backwashing flows into the inner cavity of the sewage tank 10 through the electric control valve 26. The horizontal part of the three-way pipe 28 is fixed with the electric control valve 29 on the side away from the filter assembly 27. The electric control valve 29 is connected with the inner cavity of the sewage tank 10 through a conveying pipe on one side. The impurities and cell liquid left after filtration flow into the inner cavity of the sewage tank 10 through the electric control valve 29 and the conveying pipe.

[0060] The vertical part of the three-way pipe 28 is connected with the output end of the water pump 22 through a conveying pipe. The input end of the water pump 22 is connected with the inner cavity of the water storage tank 12 through a conveying pipe. The electric control valve 29 is closed. The clean water extracted by the water pump 22 flows into the inner cavities of the multiple filter assemblies 27 through the three-way pipe 28 to backwash the multiple filter assemblies 27. At the same time, the electric control valve 26 is opened to make the liquid for backwashing flow into the inner cavity of the sewage tank 10.

[0061] In addition, as shown in Figure 5 and Figure 8 , the filter tank 21 is fixed with a driving motor 24 on one side. The output end of the driving motor 24 is fixed with a connecting shaft 241. The outer surface of the connecting shaft 241 is fixed with multiple winding rollers 242 which are adapted to the filter assemblies 27. The multiple winding rollers 242 are respectively located in the inner cavities of the filter assemblies 27. The outer surface of each winding roller 242 is fixed with two groups of connecting ropes 243. The directions of the two groups of connecting ropes 243 are opposite. The two connecting ropes 243 in the same group are close to or away from each other to make the connecting ropes 243 be distributed uniformly.

[0062] As shown in Figure 10 , the broken cell liquid flows into the three-way pipe 25 and then flows into the multiple filter assemblies 27 for tangential filtration. The remaining cell waste liquid flows into the inner cavity of the sewage tank 10.

[0063] As shown in Figure 11 , the supernatant filtered out of the broken cell liquid flows into the inner cavity of the filter tank 21 from the inner cavity of the filter assembly 27 and then flows into the inner cavity of the liquid storage tank 11 through the conveying pipe on the lower side of the filter tank 21.

[0064] As shown in Figure 12 , the water pump 22 extracts the clean water in the inner cavity of the water storage tank 12 to the vertical part of the three-way pipe 28 and then flows to the inner cavities of the multiple filter assemblies 27 through the three-way pipe 28 to backwash the filter assemblies 27 to remove the cell fragments in the filter assemblies 27. Then, the liquid for backwashing flows into the inner cavity of the sewage tank 10.

[0065] Further, as shown in Figures 7-9As shown, the filter assembly 27 comprises a fixed ring 271 inlaid and fixed in the inner part of the fixed block 23, the inner surface of the fixed ring 271 is provided with two symmetrical filter membranes 272 and two symmetrical filter screens 273, the two fixed rings 271 are fixedly connected with the fixed ring 271, for filtering impurities in the cell liquid, the two filter screens 273 are located inside the two filter membranes 272, and the two filter screens 273 are connected with the connecting shaft 241 through the one-way bearing, so that the filter screen 273 can rotate in one direction, the two filter screens 273 are used for filtering cell fragments in the cell liquid, and the filter screen 273 is fixedly connected with the connecting shaft 241, so that the filter screen 273 can rotate;

[0066] Among them, the inner surface of the fixed ring 271 is fixed with two groups of symmetrical brush strips 276, the same group of brush strips 276 has a plurality of brush strips 276, and the same group of brush strips 276 is close to the surface of the same side filter screen 273, the side close to the filter screen 273 of the brush strip 276 is the bristle, in the process of rotating the filter screen 273, the bristles on the brush strip 276 can clean the cell fragments on the filter screen 273, avoid that the cell fragments block the filter screen 273, cooperate with the backwashing to make the cell fragments on the filter screen 273 be cleaned, thereby improving the filtering effect of the filter screen 273;

[0067] Then, the inner surface of the fixed ring 271 is fixed with two symmetrical mounting plates 274 through the supporting spring, the supporting spring is fixed on the inner surface of the fixed ring 271, the supporting spring is fixed with the mounting plate 274, the two mounting plates 274 are fixedly connected with the two groups of connecting ropes 243 close to each other, the same group of two connecting ropes 243 is fixedly connected with the mounting plate 274, through the rotation of the winding roller 242, the two connecting ropes 243 can pull the mounting plate 274 to one side of the center of the fixed ring 271, in the process of rotating the winding roller 242, because the filter screen 273 is connected with the one-way bearing and the filter screen 273 has resistance at the connection with the fixed ring 271, the filter screen 273 cannot rotate;

[0068] Among them, the side close to each other of the two mounting plates 274 is slidably connected with two symmetrical moving plates 275, the inner surface of the fixed ring 271 is provided with a sliding groove matched with the moving plate 275, one side of the moving plate 275 slides in the sliding groove and abuts against the side wall of the sliding groove, so that the liquid cannot flow, one side of the two moving plates 275 close to each other is fixedly connected with the fixed plate 278, one side of the two fixed plates 278 close to each other is fixedly connected with the connecting spring 277, the two connecting springs 277 are fixedly connected with the moving plate 275, in the process that the mounting plate 274 moves to one side of the center of the fixed ring 271, the two moving plates 275 move to both sides under the action of the connected connecting spring 277, so that the two moving plates 275 are always in contact with the side wall of the sliding groove on the fixed ring 271;

[0069] The two moving plates 275 on the same side are fixed with an extension sheet, so that the two moving plates 275 can be on the same straight line, preventing the flow of liquid from being hindered by the gap between the two moving plates 275;

[0070] By rotating the connecting shaft 241, the two groups of connecting ropes 243 are wound, thereby pulling the installation plates 274 on both sides to move, reducing the cross-sectional area of the liquid flow between the two installation plates 274, thereby increasing the flow rate of the liquid, and better washing away the cell fragments and impurities on the filter screen 273 and the filter membrane 272 during backwashing, thereby reducing the frequency of replacing the filter membrane due to the clogging of the filter membrane 272, and further making the filter membrane 272 and the filter screen 273 cleaner, making the filtered supernatant cleaner, and thereby improving the quality of the antibacterial peptide.

[0071] Embodiment two

[0072] This embodiment is a further limitation of the concentration component 30 based on embodiment one, to achieve the purpose of increasing shear force through the spiral flow channel 36 to avoid the decrease of flux due to adsorption of impurities on the surface of the ultrafiltration membrane.

[0073] Specifically, as shown in Figure 13 — Figure 15 The concentration component 30 includes a concentration tank 31 fixed on the upper side of the water storage tank 12, a water pump three 32 fixed on the upper side of the concentration tank 31, a concentration pipe 34 fixed in the inner cavity of the concentration tank 31, an ultrafiltration membrane assembly 35 fixed in the inner cavity of the concentration pipe 34, the ultrafiltration membrane assembly 35 including two installation circular plates and a plurality of ultrafiltration membrane filaments, the input pipe 33 and the output pipe 37 are respectively fixed on the two sides of the ultrafiltration membrane assembly 35, the input pipe 33 and the output pipe 37 are directly connected with the ultrafiltration membrane assembly 35, the spiral flow channel 36 is fixed between the input pipe 33 and the output pipe 37 and located inside the ultrafiltration membrane assembly 35, the spiral flow channel 36 is spiral, which can make the liquid entering the inside of the ultrafiltration membrane assembly 35 from the input pipe 33 spiral, thereby enhancing the shear force of the liquid, the high-speed fluid forms a turbulent flow in the spiral structure, destroys the static boundary layer on the membrane surface, reduces the local concentration accumulation of the antibacterial peptide on the membrane surface, reduces the concentration polarization, and avoids the decrease of filtration efficiency caused by concentration gradient;

[0074] The output end of the water pump one 13 is communicated with the inner cavity of the input pipe 33 through the conveying pipe, the input end of the water pump one 13 is communicated with the inner cavity of the liquid storage tank 11 through the conveying pipe, and the water pump one 13 conveys the filtered supernatant in the inner cavity of the liquid storage tank 11 to the inner cavity of the input pipe 33 for concentration;

[0075] Next, the outer surface of the concentration pipe 34 is fixed with a sewage drainage pipe 39 near the input pipe 33, which communicates with the inner cavity of the concentration pipe 34, the ultrafiltration membrane assembly 35 is located in the inner cavity of the concentration pipe 34, and the ultrafiltration membrane assembly 35 is spaced apart from the two side walls of the inner cavity of the two concentration pipes 34, the sewage drainage pipe 39 is located between the ultrafiltration membrane assembly 35 and the concentration pipe 34, and the sewage drainage pipe 39 is fixed with a three-way electric control valve 391 at the end away from the input pipe 33, the three-way electric control valve 391 is three-way, and the direction of liquid flow can be controlled.

[0076] As shown in Figure 18 , the horizontal part of the three-way electric control valve 391 is communicated with the inner cavity of the water storage tank 12 through a conveying pipe, the clean water generated in the ultrafiltration process flows through the inner cavity of the water storage tank 12 through the sewage drainage pipe 39, the other side of the horizontal part of the three-way electric control valve 391 is communicated with the inner cavity of the sewage tank 10 through a conveying pipe, and the waste liquid generated in the backwashing process flows into the inner cavity of the sewage tank 10 through the sewage drainage pipe 39.

[0077] As shown in Figure 16 , during the ultrafiltration process, the liquid enters from the input pipe 33, flows through the spiral flow channel 36 to make the clean water and impurities move tangentially along the membrane wire, the clean water flows to the side of the input pipe 33, is conveyed to the inner cavity of the water storage tank 12 through the sewage drainage pipe 39 and the conveying pipe, and the concentrated liquid is conveyed to the purification assembly 40 for purification through the output pipe 37.

[0078] In addition, the outer surface of the concentration pipe 34 is fixed with a water inlet pipe 38 near the output pipe 37, which communicates with the inner cavity of the concentration pipe 34, the water inlet pipe 38 is located in the space between the ultrafiltration membrane assembly 35 and the concentration pipe 34 on the side of the output pipe 37, the water inlet pipe 38 is connected with the output end of the water pump three 32, and the input end of the water pump three 32 is communicated with the inner cavity of the water storage tank 12 through a conveying pipe.

[0079] As shown in Figure 17 , the water pump three 32 can extract the clean water in the inner cavity of the water storage tank 12 to backwash the ultrafiltration membrane assembly 35, so as to avoid the problem of flux decline caused by membrane blockage.

[0080] It should be noted that the water pump one 13, the driving motor 24, the three-way pipe one 25, the electric control valve one 26, the three-way pipe two 28, the electric control valve two 29, the water pump three 32, the ultrafiltration membrane assembly 35, the three-way electric control valve 391 and the purification assembly 40 in the present application are all prior art, and their installation mode and control method also belong to conventional design, which will not be described in detail.

[0081] The working principle of the present application: the device is an antibacterial peptide concentration and purification device, the tangential filtration of the broken cell liquid can be realized through the filter component 20, the deposition of macromolecular substances such as cell fragments and lipids on the surface of the membrane is avoided, the backwashing of the multiple filter assemblies 27 can be realized, so that the cell fragments adhered to the surface of the membrane in the filter assembly 27 is reduced, the problem of frequent replacement due to the blockage of the filter membrane 272 is avoided, meanwhile, the impurities in the filtered supernatant are less, and the influence on the product quality is reduced;

[0082] Specifically, as shown in Figure 10 , the broken cell liquid of the disc centrifuge enters the inner cavity of the three-way pipe one 25, at this time the electric control valve one 26 is in a closed state, the broken cell liquid flows from the three-way pipe one 25 to the inner cavity of the filter assembly 27, and then flows to the next filter assembly 27 through the connecting pipe, thereby forming a tangential filtration system, in the process of tangential flow of the broken cell liquid, the tangential flow liquid flows parallel to the membrane surface to generate high shear force, which washes the membrane surface and reduces the deposition of macromolecular substances such as cell fragments and lipids on the membrane surface, finally, the filtered cell liquid flows from the horizontal part of the three-way pipe two 28 to the electric control valve two 29, and then flows to the inner cavity of the sewage tank 10 through the conveying pipe connected to the electric control valve two 29;

[0083] Next, as shown in Figure 11 , in the process of the flow of the broken cell liquid, the cell liquid enters the inner cavity of the filter assembly 27, first the filter screens 273 on both sides filter the macromolecular impurities, then the filter membrane 272 blocks the small molecular impurities, finally the antibacterial peptide supernatant flows from the inner cavity of the filter assembly 27 to the inner cavity of the filter tank 21, and then flows to the inner cavity of the liquid storage tank 11 through the conveying pipe on the lower side of the filter tank 21;

[0084] The tangential filtration system is backwashed, as shown in Figure 12 , the clean water in the inner cavity of the water storage tank 12 is pumped by the water pump two 22 and conveyed to the three-way pipe two 28, at this time the electric control valve two 29 is closed, the clean water in the three-way pipe two 28 is conveyed to the tangential filtration system composed of the multiple filter assemblies 27 and the connecting pipe, which is opposite to the flow path of the broken cell liquid, thereby forming backwashing, which can carry out the impurities in the inner cavity of the filter assembly 27, and then flows to the electric control valve one 26 from the vertical part of the three-way pipe one 25, and then flows into the inner cavity of the sewage tank 10 from the conveying pipe of the electric control valve one 26, through the backwashing, the flux of the filter membrane 272 and the filter screen 273 can be restored, the membrane pollution is inhibited, the service life of the membrane is prolonged, and the time of shutdown can be reduced without disassembly for washing, thereby improving the filtration efficiency;

[0085] In addition, during backwashing, first, the connecting shaft 241 is driven to rotate forward by the motor 24, so that the filter screen 273 rotates, and the brush strip 276 is fixed, so that the brush strip 276 can clean the surface of the filter screen 273, and the cell fragments on the filter screen 273 are loosened, then the connecting shaft 241 is driven to rotate reversely by the motor 24, at this time, the filter screen 273 no longer rotates, the winding roller 242 rotates, and the two mounting plates 274 of the filter assembly 27 are moved towards each other through the two groups of connecting ropes 243, so that the liquid flow area between the two filter assemblies 27 is reduced, the liquid flow speed is improved, and the impurities on the filter screen 273 and the filter membrane 272 can be further washed away, and the backwashing effect is further improved.

[0086] During the concentration of the antibacterial peptide supernatant, the antibacterial peptide supernatant is formed into turbulent flow in the spiral flow channel 36 through the concentration component 30, the static boundary layer on the membrane surface is destroyed, the local concentration accumulation of the antibacterial peptide on the membrane surface is reduced, the concentration polarization is reduced, and the decline of the filtration efficiency caused by the concentration gradient is avoided.

[0087] Specifically, as shown in the figure, Figure 16 The fixed block 23 delivers the antibacterial peptide supernatant in the inner cavity of the liquid storage tank 11 to the inner cavity of the input pipe 33, then forms turbulent flow through acceleration of the spiral flow channel 36, then performs ultrafiltration through the ultrafiltration membrane assembly 35, so that water flows to the side of the sewage and drainage pipe 39, then is discharged to the inner cavity of the water storage tank 12 through the sewage and drainage pipe 39, and the antibacterial peptide concentrate is delivered from the output pipe 37 to the purification assembly 40 for purification.

[0088] Then, as shown in the figure, Figure 17 The water pump three 32 delivers the clean water in the inner cavity of the water storage tank 12 to the water inlet pipe 38, and then delivers the clean water to the inner cavity of the concentration pipe 34 through the three-way pipe two 28, so as to backwash the ultrafiltration membrane assembly 35. The waste liquid of backwashing flows to the inner cavity of the sewage tank 10 through the sewage and drainage pipe 39 and the three-way electric valve 391. The flux of the ultrafiltration membrane assembly 35 can be restored through backwashing of the ultrafiltration membrane assembly 35, and the filtration efficiency is improved.

[0089] The above is only the preferred specific embodiment of the application, but the protection scope of the application is not limited to this. Any person skilled in the art can make equivalent replacement or change according to the technical concept of the antibacterial peptide concentration and purification device and the application concept within the technical range disclosed by the application, which should be covered in the protection scope of the application.

Claims

1. A device for concentrating and purifying an antibacterial peptide, comprising a sewage tank (10) and a storage tank (11), characterized in that: The sewage tank (10) is fixed with a filtering part (20) on the upper side, the liquid storage tank (11) is fixed with a water storage tank (12) on one side, the liquid storage tank (11) is fixed with a water pump (13) on the upper side, the water storage tank (12) is fixed with a concentration part (30) on the upper side, and the concentration part (30) is installed with a purification assembly (40) on one side. The filtering part (20) comprises a filtering tank (21) and a water pump (22) fixedly connected with the filtering tank (21), the filtering tank (21) is communicated with the inner cavity of the liquid storage tank (11) through a plurality of conveying pipes on the lower side, a plurality of fixed blocks (23) are fixed in the inner cavity of the filtering tank (21) and uniformly distributed, a plurality of filtering assemblies (27) are embedded in the inner part of the fixed blocks (23), two adjacent filtering assemblies (27) are connected through a connecting pipe, and two filtering assemblies (27) on both sides are fixedly connected with a three-way pipe (25) and a three-way pipe (28) respectively. The filtering tank (21) is fixed with a driving motor (24) on one side, the output end of the driving motor (24) is fixed with a connecting shaft (241), a plurality of winding rollers (242) matched with the filtering assemblies (27) are fixed on the outer surface of the connecting shaft (241), and two groups of connecting ropes (243) are fixed on the outer surface of each winding roller (242). The filtering assembly (27) comprises a fixed ring (271), two symmetrical filter membranes (272) and two symmetrical filter screens (273) are arranged on the inner surface of the fixed ring (271), two filter screens (273) are located on the inner side of the two filter membranes (272), the two filter screens (273) are fixedly connected with the connecting shaft (241), two groups of symmetrical brush strips (276) are fixed on the inner surface of the fixed ring (271), and the brush strips (276) in the same group are tightly attached to the surface of the filter screen (273) on the same side. The inner surface of the fixed ring (271) is fixed with two symmetrical mounting plates (274) through supporting springs, the two mounting plates (274) are fixedly connected with two groups of connecting ropes (243) located close to each other, and two symmetrical moving plates (275) are slidably connected to the side close to each other of the two mounting plates (274). A sliding groove matched with the moving plate (275) is formed in the inner surface of the fixed ring (271), the side of each of the two moving plates (275) on the same side is fixed with a fixed plate (278), the side close to each other of the two fixed plates (278) is fixed with a connecting spring (277), and the connecting spring (277) is fixedly connected with the moving plate (275). A tangential filtering flow loop is formed between the plurality of filtering assemblies (27) through the three-way pipe (25) and the connecting pipe, and the water pump (22) can extract clean water from the water storage tank (12), so that the plurality of filtering assemblies (27) can be backwashed.

2. The apparatus for concentrating and purifying an antibacterial peptide according to claim 1, wherein The tee pipe one (25) vertical part is connected with electric control valve one (26), one side of electric control valve one (26) is communicated with the inner cavity of sewage tank (10) through the conveying pipe, the tee pipe two (28) horizontal part is fixed with electric control valve two (29) on the side away from the filter assembly (27), one side of electric control valve two (29) is communicated with the inner cavity of sewage tank (10) through the conveying pipe, the tee pipe two (28) vertical part is connected with the output end of water pump two (22) through the conveying pipe, the input end of water pump two (22) is communicated with the inner cavity of water storage tank (12) through the conveying pipe.

3. The apparatus for concentrating and purifying an antibacterial peptide according to claim 2, wherein The concentration component (30) includes the concentration tank (31) fixed on the upper side of water storage tank (12), the water pump three (32) is fixed on the upper side of concentration tank (31), the concentration tank (31) is fixed with concentration pipe (34) in the inner cavity, the concentration pipe (34) is fixed with ultrafiltration membrane assembly (35) in the inner cavity, the input pipe (33) and output pipe (37) are respectively fixed on the two sides of ultrafiltration membrane assembly (35), the spiral flow channel (36) is fixed between the input pipe (33) and output pipe (37) and located in the inside of ultrafiltration membrane assembly (35), the output end of water pump one (13) is communicated with the inner cavity of input pipe (33) through the conveying pipe, the input end of water pump one (13) is communicated with the inner cavity of liquid storage tank (11) through the conveying pipe.

4. The apparatus for concentrating and purifying an antibacterial peptide according to claim 3, wherein The outer surface of concentration pipe (34) is fixed with sewage drainage pipe (39) communicated with the inner cavity of concentration pipe (34) on the side close to input pipe (33), the end of sewage drainage pipe (39) away from input pipe (33) is fixed with tee electric control valve (391), one side of tee electric control valve (391) horizontal part is communicated with the inner cavity of water storage tank (12) through the conveying pipe, the other side of tee electric control valve (391) horizontal part is communicated with the inner cavity of sewage tank (10) through the conveying pipe.

5. The apparatus for concentrating and purifying an antibacterial peptide according to claim 4, wherein The outer surface of concentration pipe (34) is fixed with water inlet pipe (38) communicated with the inner cavity of concentration pipe (34) on the side close to output pipe (37), the water inlet pipe (38) is connected with the output end of water pump three (32), the input end of water pump three (32) is communicated with the inner cavity of water storage tank (12) through the conveying pipe.

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