Biological bacterium active material as well as preparation method and application thereof

Abstract

The invention discloses a biological bacterium active material as well as a preparation method and application thereof. The raw materials of the biological bacterium active material comprise the following components in percentage by mass of Japanese premna herb: 20-50% of a bioactive filler, 5-20% of an oxygen production filler, 10-30% of medical stone, 0-30% of ceramic particles, 0-30% of quartz sand, 5-20% of aluminum phosphate cement, 1-3% of a water reducing agent, 10-30% of a biological bacterium spore composition and 3-5% of vegetable gum. The biological bacterium active material provided by the invention can effectively maintain a good water ecosystem, and can be widely applied to the field of water quality purification.

Description

Technical Field

[0001] The present invention relates to the field of water treatment, in particular to the field of microbial water purification, and specifically to a biological bacteria active material and a preparation method and application thereof. Background Art

[0002] Regular water changes are necessary for home ornamental fish tanks. Over time, ammonia nitrogen and nitrate levels increase, blue-green algae grow on the glass, the water becomes turbid, and harmful bacteria, such as Aeromonas wieldingii, Vibrio, and Streptococcus, increase in number, and can kill fish, creating an unsightly appearance. Regular water changes increase labor and costs, while also polluting the environment. Larger ornamental fish ponds also face the same challenge, requiring frequent water changes. Each change can require several tons of water, sometimes hundreds of tons for larger ponds. This wastes water resources and pollutes rivers through the discharge of wastewater. Therefore, it's crucial to minimize water changes and ensure the survival of ornamental fish.

[0003] In addition, small rivers such as town rivers and urban rivers often have bad odors due to poor fluidity. The water quality is often black and contains blue algae. In addition, the organic matter and heavy metals in them seriously exceed the standard BOD5>10mg / L. Therefore, an effective solution is needed to improve river water quality and restore the ecology.

[0004] Existing bio-cement blocks for water purification suffer from poor biological purification performance because most of the pores in the concrete are closed, preventing bacteria from surviving. Ordinary cement is highly alkaline, severely hindering the growth and survival of bacteria. Furthermore, the amount of bacteria required increases costs, as does the high proportion of pores in a cubic meter where bacteria can thrive, which only accounts for 10%-20%. Summary of the Invention

[0005] The main purpose of the present invention is to provide a biological bacteria active material and its preparation method and application, so as to overcome the shortcomings of the prior art.

[0006] To achieve the aforementioned object of the invention, the technical solutions adopted by the present invention include:

[0007] One aspect of the present invention provides a biological bacteria active material, the raw materials of which include the following components calculated by mass percentage: 20-50% of biological active filler, 5%-20% of oxygen-generating filler, 10-30% of medical stone, 0-30% of ceramic particles, 0-30% of quartz sand, 5-20% of aluminum phosphate cement, 1-3% of water reducer, 10-30% of biological bacteria spore composition and 3-5% of plant gum;

[0008] The raw materials of the bioactive filler include the following components calculated by mass percentage: 30-70% aluminum phosphate cement, 20-60% volcanic mud, 5-40% medical stone powder, 2-10% active additives, 0.5-2% agar, 2-5% bran, 3-8% bagasse, 0.1-0.5% yeast extract and 0.1-1% dihydrogen phosphate; the active additives include any one of far-infrared powder and silica fume, or a combination of two of them.

[0009] Another aspect of the present invention provides a method for preparing the aforementioned biological active material, which comprises: mixing a biologically active filler, an oxygen-generating filler, medical stone, ceramic particles that may or may not be added, quartz sand that may or may not be added, aluminum phosphate cement, a biological spore composition, plant gum, a water reducer, and water to obtain a mixture; and molding the mixture to obtain the biological active material.

[0010] Another aspect of the present invention also provides the use of the aforementioned biological bacteria active material in water purification.

[0011] Another aspect of the present invention provides a water purification method, which comprises: immersing the aforementioned biological bacteria active material in the water to be purified.

[0012] Compared with the prior art, the present invention has at least the following advantages:

[0013] The biological active material provided by the present invention can be placed in water to stimulate the biological bacteria in the spore state to transform into biologically active bacteria. The oxygen-generating filler provides oxygen for the biological bacteria. The biologically active bacteria enter the water and use harmful substances in the water as food (ammonia nitrogen, nitrates, phosphates, organic matter, etc.), which is beneficial to preventing the formation of cyanobacteria and reducing the growth of harmful bacteria. The biologically active bacteria reproduce and grow in the bio-block, which can effectively maintain a good water ecosystem and can be widely used in the field of water purification. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments recorded in the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0015] Figure 1 This is a photo of the biological bacteria active material prepared in Example 1 of the present invention. DETAILED DESCRIPTION

[0016] The present invention will be more fully understood by reading the following detailed description. However, it should be understood that the detailed description disclosed below is merely exemplary of the present invention, and that the present invention may be embodied in a variety of forms. Therefore, the specific functional details disclosed herein should not be construed as limiting, but rather as a basis for the claims and as a representative basis for teaching those skilled in the art to variously employ the present invention in virtually any appropriately detailed embodiment.

[0017] As one aspect of the technical solution of the present invention, the raw materials of a biological bacteria active material include the following components calculated by mass percentage: 20-50% of biological active filler, 5%-20% of oxygen-generating filler, 10-30% of medical stone, 0-30% of ceramic particles, 0-30% of quartz sand, 5-20% of aluminum phosphate cement, 1-3% of water reducer, 10-30% of biological bacteria spore composition and 3-5% of plant gum;

[0018] The raw materials of the bioactive filler include the following components calculated by mass percentage: 30-70% aluminum phosphate cement, 20-60% volcanic mud, 5-40% medical stone powder, 2-10% active additives, 0.5-2% agar, 2-5% bran, 3-8% bagasse, 0.1-0.5% yeast extract and 0.1-1% dihydrogen phosphate; the active additives include any one of far-infrared powder and silica fume, or a combination of two of them.

[0019] In some embodiments, the bioactive filler is spherical particles with a particle size of 0.4-0.6 cm.

[0020] In some embodiments, the preparation method of the bioactive filler includes: adding aluminum phosphate cement, volcanic mud, medical stone powder, active additives, agar, bran, bagasse, yeast paste and dihydrogen phosphate into a round pot granulator and spraying water to make balls to obtain the bioactive filler (energy balls).

[0021] In some preferred embodiments, the particle size of the volcanic mud is 250-400 mesh.

[0022] In some preferred embodiments, the particle size of the active additive is 250-400 mesh.

[0023] In some preferred embodiments, the particle size of the medical stone powder in the raw material of the bioactive filler is 250-400 meshes.

[0024] In some preferred embodiments, the agar is in powder form, and its particle size is 200-320 mesh.

[0025] In some preferred embodiments, the yeast extract is in powder form, and its particle size is 200-320 mesh.

[0026] In some preferred embodiments, the particle size of the bran is 200-320 mesh.

[0027] In some preferred embodiments, the particle size of the bagasse is 200-320 mesh.

[0028] In some preferred embodiments, the process conditions of the water-spraying pelletizing include: the rotation speed of the round pot granulator is 10-35 r / min, the temperature of the water-spraying pelletizing is room temperature, the time of the water-spraying pelletizing is 20-30 min, and the amount of water sprayed is 18-35% of the mass of the dry material.

[0029] In the present invention, bioactive fillers (energy balls) provide energy and skeletons, medical stone is used to build houses (biological bacteria breeding space) and provide metal ions, waste ceramic particles and quartz sand are used to build houses, aluminum phosphate cement improves strength, water reducers are used to reduce the addition of water, and plant gum can enhance the structural stability of biological bacteria active materials within a certain period of time, and it can slowly degrade naturally. In this process, some components that are beneficial to the growth of biological bacteria can be released, and the slow disintegration of biological bacteria active materials can be promoted, which is beneficial to environmental protection.

[0030] In the present invention, aluminum phosphate cement is used to increase the strength of the ball and is neutral and does not harm the bacteria; volcanic mud provides the excitation energy of the bacteria to increase the activity and improve the strength of the ball by precipitating some metal ions Fe. 2+ 、Mn 2+ 、Zn 2+ Ions required for the reproduction of other biological bacteria; medical stone powder can precipitate some metal ions Fe 2+ 、Mn 2+ 、Zn 2+ Ions such as ions are needed for bacterial growth; far-infrared powder is used to stimulate energy; and silica fume can stimulate energy and release infrared waves. Its inherent polarization properties release far-infrared waves that can shake large water molecules into smaller ones, achieving the effect of activated water. Activated water has excellent penetrating and dissolving power, which has a significant effect on the metabolism of biological bacteria.

[0031] In the present invention, the plant fibers contained in bagasse and bran are combined with cement, volcanic mud, medical stone powder, etc., which can, on the one hand, make the biological active filler have a loose and porous structure while maintaining a stable structure for a long time; on the other hand, it can slowly release components in the biological active material that are beneficial to promoting the growth of biological bacterial spores into mycelium, thereby promoting the purification function and avoiding excessive reproduction of biological bacterial spores.

[0032] In some embodiments, the preparation method of the oxygen-generating filler includes: uniformly mixing zeolite powder, polymer gel and green algae to obtain a mixed solution, dripping the mixed solution into a calcium chloride solution to form gel beads, and solidifying to obtain the oxygen-generating filler.

[0033] In some preferred embodiments, the polymer gel includes sodium alginate and polyvinyl alcohol.

[0034] In some preferred embodiments, the preparation method of the oxygen-generating filler comprises: adding 4 to 10 parts of 0.5-1 μm zeolite powder and 2 to 4 parts of polymer gel (such as sodium alginate or polyvinyl alcohol) to 100 parts of water, ultrasonically dispersing them uniformly, and adding a concentration of 10 7 cells-10 8 30-60 parts of green algae water suspension with a concentration of 10 cells / mL are stirred evenly, and then dropped into a calcium chloride solution with a concentration of 2wt% to 4wt% to form 2-4mm gel beads, which are cured for 30min-50min to prepare oxygen-generating filler.

[0035] In some more specific embodiments, the preparation method of the oxygen-generating filler comprises: adding 4-10 g of 0.5-1 μm zeolite powder and 2-4 g of sodium alginate or polyvinyl alcohol to 100 ml of water, ultrasonically dispersing them uniformly, and then adding a 10% 7 cells-10 8 30-60 ml of green algae water suspension with a concentration of 10 cells / mL is stirred evenly, and then dripped into a calcium chloride solution with a concentration of 2wt%-4wt% to form 2-4 mm gel beads, which are cured for 30min-50min to obtain oxygen-generating filler.

[0036] In this invention, the oxygen-generating filler, made from zeolite, acts as a porous material, acting as an adsorbent, increasing specific surface area and providing mechanical strength. Green algae cells are adsorbed onto the surface and within pores and embedded within a composite matrix of sodium alginate or polyvinyl alcohol. In the presence of light, the green algae produce oxygen, providing sufficient oxygen for the microbial life. At night, in the absence of light, the green algae breathe, slightly reducing oxygen levels in the water. However, the small amount of green algae does not affect fish growth or microbial life. Lights can also be turned on at night to increase oxygen production.

[0037] In some embodiments, the particle size of the medical stone in the raw material of the biological bacteria active material is 20 mesh to 60 mesh.

[0038] In some embodiments, the ceramic particles have a particle size of 20 mesh to 60 mesh.

[0039] In some embodiments, the quartz sand has a particle size of 20 mesh to 60 mesh.

[0040] In some embodiments, the water reducer includes but is not limited to a polycarboxylate water reducer.

[0041] In some embodiments, the biological bacterial spore composition includes 30-60 wt% of Bacillus subtilis spores, 15-30 wt% of Bacillus thuringiensis spores, 20-40 wt% of Bacillus sphaericus spores, 5-15 wt% of yeast and 5-25 wt% of lactic acid bacteria.

[0042] In some embodiments, the biological active material is a block material with a porosity of 30-45% and a pore size of 20 μm-5 mm.

[0043] As another aspect of the technical solution of the present invention, the method for preparing the aforementioned biological bacteria active material includes: mixing a biologically active filler, an oxygen-generating filler, medical stone, ceramic particles (optionally added or not added), quartz sand (optionally added or not added), aluminum phosphate cement, a biological bacteria spore composition, plant glue, a water reducer, and water to obtain a mixture; and molding the mixture to obtain the biological bacteria active material;

[0044] In some embodiments, the water content in the mixture is 8-15% by mass.

[0045] In some embodiments, the process conditions of the molding treatment include: placing the mixed material in a mold for drying and molding at room temperature.

[0046] In some specific embodiments, the method for preparing the biological active material comprises the following steps:

[0047] 1) Aluminum phosphate cement, volcanic mud, far infrared powder, medical stone powder, silica fume, agar, bran, bagasse, yeast extract, and dihydrogen phosphate are mixed and added to a round pot granulator and water spraying is started to form balls. Balls are formed into balls with a diameter of about 0.4 cm to 0.6 cm according to the ball making time, and are dried in the shade for later use to obtain a bioactive filler;

[0048] 2) Add 4-10g of 0.5-1μm zeolite powder and 2-4g of sodium alginate or polyvinyl alcohol to 100ml of water, disperse them evenly by ultrasonic, and add 10 7 cells-10 8 30-60 ml of a green algae water suspension with a concentration of 10 cells / mL was stirred evenly, and then dropped into a calcium chloride solution with a concentration of 2wt%-4wt% to form 2-4 mm gel beads, which were cured for 30-50 minutes to obtain an oxygen-generating filler.

[0049] 3) The prepared bioactive filler, oxygen-generating filler, medical stone, ceramic particles, quartz sand, aluminum phosphate cement, plant glue, water reducer, and biological bacteria spore composition are mixed, water is added, and the mixture is rapidly stirred. The mixture is poured into a 10 cm × 10 cm × 20 cm plastic mold, dried in a drying oven at 40-50° C., and demolded to form a finished product to obtain a biological bacteria active material.

[0050] Another aspect of the present invention relates to the application of the aforementioned bioactive materials in water purification, such as in fish tanks, large ornamental fish ponds, rivers, sewers, and the like.

[0051] As another aspect of the technical solution of the present invention, a water purification method thereof comprises: immersing the aforementioned biological bacteria active material in the water to be purified.

[0052] The bioactive fillers (energy balls) in the bioactive material (bioblock) provided by the present invention are connected to form a solid structure through aluminum phosphate cement. The joints between the balls are connected and communicated with each other. Quartz sand, ceramic particles and medical stone are accumulated at the joints of the balls to form a large number of micron and millimeter pores that are communicated with each other, forming a nest for the survival of biobacteria. After the bioblock encounters water, the energy balls emit far-infrared waves, converting the surrounding polymerized water molecules into small molecular water, and increasing the dissolved oxygen content, stimulating the biobacterial spores to become active bacteria that use harmful substances in the water as food, making the water clearer. Volcanic mud and medical stone precipitate Fe 2+ Mg 2+ , Ca 2 + 、Mn 2+ The oxygen-generating filler produces oxygen under the photosynthesis of green algae, which provides oxygen for the biological bacteria.

[0053] The present invention is further illustrated by examples, which are not intended to limit the invention to these examples. The reagents and raw materials used in the following examples are commercially available. Experimental methods where specific conditions are not specified were generally performed under conventional conditions or according to the manufacturer's recommendations. For example, the silica fume used in the following examples is furnace dust from steel mills. Its mineral composition is primarily single-crystalline silicon, emitting long wavelengths of 3 μm to 1 mm.

[0054] Example 1

[0055] A method for preparing a biological bacteria active material comprises the following steps:

[0056] Preparation of bioactive filler (energy ball): 40 kilograms of aluminum phosphate cement, 30 kilograms of volcanic mud, 25 kilograms of medical stone powder, 5 kilograms of far-infrared powder, 1.5 kilograms of agar, 3.5 kilograms of bran, 4 kilograms of bagasse, 0.5 kilogram of yeast extract and 0.5 kilogram of dihydrogen phosphate are added into a round pot granulator. In the round pot granulator, rotating speed is 15r / min and mixes 20 minutes. And spray water at normal temperature, the water spraying amount is about 18% of dry material. When the diameter of the ball reaches 0.5cm, sieve and leave 0.4cm~0.6cm ball. Little ball returns to the round pot granulator again to make the bioactive filler, and carries out drying.

[0057] Preparation of oxygen-generating filler: 6 g of 0.5-1 μm zeolite powder and 2 g of sodium alginate were added into 100 ml of water, ultrasonically dispersed evenly, and then added into the mixture at a concentration of 10 7 40 ml of a green algae water suspension with a concentration of 10 cells / mL was stirred evenly, and then dropped into a 2 wt % calcium chloride solution to form 2-4 mm gel beads, which were cured for 30 minutes to prepare an oxygen-generating filler.

[0058] Preparation of biological active material: 36 kg of energy balls, 8 kg of oxygen-generating filler, 18 kg of medical stone particles, 25 kg of quartz sand, 2 kg of polycarboxylate water reducer, 18 kg of aluminum phosphate cement, 18 kg of biological spore mixture (including 50% by weight of Bacillus subtilis, 15% by weight of Bacillus thuringiensis, 20% by weight of Bacillus sphaericus, 8% by weight of yeast, and 7% by weight of lactic acid bacteria), 5 kg of sesbania gum, and 15 kg of water were stirred and mixed, and the mixture was formed in a 10 cm × 10 cm × 20 cm mold and then dried at about 50°C to obtain the biological active material (bioblock), as shown in the photo. Figure 1 shown.

[0059] Example 2

[0060] A method for preparing a biological bacteria active material comprises the following steps:

[0061] The preparation of bioactive filler (energy ball): 48 kilograms of aluminum phosphate cement, 30 kilograms of volcanic mud, 18 kilograms of medical stone powder, 4 kilograms of silica fume, 1 kilogram of agar, 4 kilograms of bran, 4.4 kilograms of bagasse, 0.3 kilogram of yeast extract and 0.3 kilogram of dihydrogen phosphate are added in the round pot granulator. In the round pot granulator, rotating speed is that 25r / min mixes 25 minutes, and sprays water at normal temperatures, the water spray rate is about 20% of the dry material. When the diameter of the ball reaches 0.5cm, sieve and leave 0.4cm~0.6cm ball, little ball returns the round pot granulator again, makes the bioactive filler, and carries out drying.

[0062] Preparation of oxygen-generating filler: add 8g of 0.5-1μm zeolite powder and 4g of polyvinyl alcohol to 100ml of water, disperse them evenly with ultrasonic, and add 10 750 ml of a green algae water suspension with a concentration of 10 cells / mL was stirred evenly, and then dropped into a 2 wt % calcium chloride solution to form 2-4 mm gel beads, which were cured for 40 minutes to prepare an oxygen-generating filler.

[0063] Preparation of biological bacteria active material: 30 kg of energy balls, 10 kg of oxygen-generating filler, 25 kg of medical stone, 20 kg of quartz sand, 2 kg of polycarboxylate water reducer, 15 kg of aluminum phosphate cement, plus 23 kg of biological bacteria spore mixture (of which the mass content is 55% Bacillus subtilis, 15% Bacillus thuringiensis, 20% sphaericus, 5% yeast and 5% lactic acid bacteria), 5 kg of guar gum, and 15 kg of water are stirred and mixed in a 10 cm × 10 cm × 20 cm mold, and then dried at about 40°C to obtain the biological bacteria active material (bioblock).

[0064] Example 3

[0065] A method for preparing a biological bacteria active material comprises the following steps:

[0066] Preparation of bioactive filler (energy ball): 58 kilograms of aluminum phosphate cement, 24 kilograms of volcanic mud, 14 kilograms of medical stone powder, 4 kilograms of far-infrared powder, 1 kilogram of agar, 3 kilograms of bran, 5 kilograms of bagasse, 0.4 kilogram of yeast extract and 0.6 kilogram of dihydrogen phosphate are added to a round pot granulator. In the round pot granulator, the rotating speed is 25r / min and mixed for 20 minutes. At room temperature, water is sprayed, and the water spray rate is about 25% of the dry material. When the diameter of the ball reaches 0.5cm, sieve and leave balls of 0.4cm to 0.6cm. The small balls are returned to the round pot granulator to make the bioactive filler and dry.

[0067] Preparation of oxygen-generating filler: add 6g of 0.5-1μm zeolite powder and 2g of polyvinyl alcohol to 100ml of water, disperse them evenly with ultrasonic, and add 10 7 40 ml of a green algae water suspension with a concentration of 10 cells / mL was stirred evenly, and then dropped into a 2 wt % calcium chloride solution to form 2-4 mm gel beads, which were cured for 30 minutes to prepare an oxygen-generating filler.

[0068] Preparation of biological bacteria active material: 40 kg of energy balls, 15 kg of oxygen-generating filler, 25 kg of medical stone, 10 kg of quartz sand, 2 kg of polycarboxylate water reducer, 13 kg of aluminum phosphate cement, plus 20 kg of biological bacteria spore mixture (of which the mass content is 45% Bacillus subtilis, 20% Bacillus thuringiensis, 15% Bacillus sphaericus, 10% yeast and 10% lactic acid bacteria), 5 kg of soy glue, and 13 kg of water are stirred and mixed in a 10 cm × 10 cm × 20 cm mold, and then dried at about 50°C to obtain the biological bacteria active material (bioblock).

[0069] Example 4

[0070] A method for preparing a biological bacteria active material comprises the following steps:

[0071] The preparation of bioactive filler (energy ball): 45 kilograms of aluminum phosphate cement, 25 kilograms of volcanic mud, 20 kilograms of medical stone powder, 10 kilograms of far-infrared powder, 0.5 kilogram of agar, 3 kilograms of bran, 5.5 kilograms of bagasse, 0.4 kilogram of yeast extract and 0.6 kilogram of dihydrogen phosphate are added to a round pot granulator. In the round pot granulator, the rotating speed is 30r / min and mixed for 20 minutes. At normal temperatures, water is sprayed, and the water spray rate is about 18% of the dry material. When the diameter of the ball reaches 0.5cm, sieve and leave balls of 0.4cm~0.6cm. The little balls are returned to the round pot granulator to make the bioactive filler and dry.

[0072] Preparation of oxygen-generating filler: add 10g of 0.5-1μm zeolite powder and 4g of sodium alginate to 100ml of water, disperse them evenly with ultrasonic, and add 10 8 45 ml of a green algae water suspension with a concentration of 10 cells / mL was stirred evenly, and then dropped into a 3 wt % calcium chloride solution to form 2-4 mm gel beads, which were cured for 50 minutes to prepare an oxygen-generating filler.

[0073] Preparation of bio-bacterial active material: 50 kg of energy balls, 13 kg of oxygen-generating filler, 20 kg of medical stone, 12 kg of ceramic particles, 2 kg of polycarboxylate water reducer, 13 kg of aluminum phosphate cement, 16 kg of biological bacterial spore mixture (including 52% by mass of Bacillus subtilis, 20% by mass of Bacillus thuringiensis, 10% by mass of Bacillus sphaericus, 10% by mass of yeast and 8% by mass of lactic acid bacteria), 4 kg of sesbania gum, and 12 kg of water are stirred and mixed, and then formed in a 10 cm × 10 cm × 20 cm mold and dried at about 50°C to obtain the bio-bacterial active material (bioblock).

[0074] Example 5

[0075] A method for preparing a biological bacteria active material comprises the following steps:

[0076] Preparation of bioactive filler (energy ball): 35 kilograms of aluminum phosphate cement, 40 kilograms of volcanic mud, 15 kilograms of medical stone powder, 10 kilograms of silica fume, 1 kilogram of agar, 5 kilograms of bran, 4 kilograms of bagasse, 0.4 kilogram of yeast extract and 0.6 kilogram of dihydrogen phosphate are added into a round pot granulator. In the round pot granulator, rotating speed is 25r / min and mixes 20 minutes. And spray water at normal temperature, the water spraying amount is about 22% of dry material. When the diameter of the ball reaches 0.5cm, sieve and leave 0.4cm~0.6cm ball. Little ball returns to the round pot granulator again to make the bioactive filler, and carries out drying.

[0077] Preparation of oxygen-generating filler: add 10g of 0.5-1μm zeolite powder and 3g of sodium alginate to 100ml of water, disperse them evenly with ultrasonic, and add 10 7 50 ml of a green algae water suspension with a concentration of 10 cells / mL was stirred evenly, and then dropped into a 2 wt % calcium chloride solution to form 2-4 mm gel beads, which were cured for 30 minutes to prepare an oxygen-generating filler.

[0078] Preparation of biological bacteria active material: 45 kg of energy balls, 10 kg of oxygen-generating filler, 25 kg of medical stone, 8 kg of quartz sand, 2 kg of polycarboxylate water-reducing agent, 10 kg of aluminum phosphate cement, plus 25 kg of biological bacteria spore mixture (wherein the mass content of Bacillus subtilis is 45%, the mass content of Bacillus thuringiensis is 10%, the mass content of Bacillus sphaericus is 20%, the yeast is 10% and the lactic acid bacteria is 15%), 5 kg of guar gum, and 13 kg of water are stirred and mixed, and then formed in a 10 cm × 10 cm × 20 cm mold and dried at about 50°C to obtain the biological bacteria active material (bioblock).

[0079] Comparative Example 1

[0080] The preparation method of a biological bacteria active material provided in this comparative example is basically the same as that in Example 1, with the only difference being that during the preparation of the energy balls, the amount of far-infrared powder added is 15 kg.

[0081] Comparative Example 2

[0082] The preparation method of a biological bacteria active material provided in this comparative example is basically the same as that in Example 1, except that the amount of the biological bacteria spore mixture added to the raw materials for preparing the biological bacteria active material is 1 kg.

[0083] Comparative Example 3

[0084] The preparation method of a biological bacteria active material provided in this comparative example is basically the same as that in Example 1, except that no energy balls are added to the raw materials for preparing the biological bacteria active material.

[0085] Comparative Example 4

[0086] The preparation method of a biological bacteria active material provided in this comparative example is basically the same as that in Example 1, except that the energy balls in the raw materials for preparing the biological bacteria active material are replaced with far-infrared powder of the same mass.

[0087] Comparative Example 5

[0088] The preparation method of a biological bacteria active material provided in this comparative example is basically the same as that in Example 1, with the only difference being that the far infrared powder is omitted during the preparation of the energy balls.

[0089] Comparative Example 6

[0090] The preparation method of a biological bacteria active material provided in this comparative example is basically the same as that in Example 1, with the only difference being that the oxygen-generating filler is omitted.

[0091] Comparative Example 7

[0092] The preparation method of a biological bacteria active material provided in this comparative example is basically the same as that in Example 1, with the only difference being that no plant gum is added during the preparation of the energy balls.

[0093] Comparative Example 8

[0094] The preparation method of a biological bacterial active material provided in this comparative example is basically the same as that in Example 1, except that agar is not added during the preparation of the energy balls.

[0095] Comparative Example 9

[0096] The preparation method of a biological bacteria active material provided in this comparative example is basically the same as that in Example 1, with the only difference being that no bran is added during the preparation of the energy balls.

[0097] Comparative Example 10

[0098] The preparation method of a biological bacteria active material provided in this comparative example is basically the same as that in Example 1, except that no sugarcane bagasse is added during the preparation of the energy balls.

[0099] Comparative Example 11

[0100] The preparation method of a biological bacterial active material provided in this comparative example is basically the same as that in Example 1, except that no yeast paste is added during the preparation of the energy balls.

[0101] Comparative Example 12

[0102] The preparation method of a biological bacteria active material provided in this comparative example is basically the same as that in Example 1, with the only difference being that no dihydrogen phosphate is added during the preparation of the energy balls.

[0103] Comparative Example 13

[0104] The preparation method of a biological bacterial active material provided in this comparative example is basically the same as that in Example 1, except that agar, bran, bagasse, yeast extract and dihydrogen phosphate are omitted in the preparation process of the energy balls.

[0105] Performance comparison test

[0106] Fish tank water purification test:

[0107] Examples 1-5 and Comparative Examples 1-13 were subjected to water purification testing. Twenty identical ornamental goldfish were placed in four 1-cubic-meter fish tanks, each containing three bio-blocks prepared in the examples. One fish tank remained empty as a blank control. Water quality was tested in January, March, and June. The fish tanks maintained a constant water circulation system (air pump) to ensure a flowing water state. Dissolved oxygen in the water was measured at 5:00 a.m. Test results are shown in Tables 1 to 7.

[0108] Because the goldfish are fed with biological feed from Anhui Jiguang Golden Dolphin, the fish feces float on the surface and can be cleaned regularly, or a sewage system can be installed on it to automatically clean it.

[0109] Fish activity is divided into good, average, poor, and dead. According to the water quality requirements of the goldfish tank, BOD5 mg / L≤5mg / L, ammonia nitrogen ppm≤0.02ppm, nitrite ppm<0.2ppm, sodium phosphate ppm<50ppm (ideal value<20ppm), DO mg / l>5mg / l, pH value 7.0-8.0.

[0110] Table 1:

[0111]

[0112] Table 2:

[0113]

[0114] Table 3:

[0115]

[0116] Table 4:

[0117]

[0118]

[0119] Table 5:

[0120]

[0121] Table 6:

[0122]

[0123] Table 7:

[0124]

[0125] The data in Tables 1 to 7 show that the addition of far-infrared powder beyond the standard in the energy balls in Comparative Example 1 had little impact on water quality. Due to the high price of far-infrared powder, its addition remained within the specified range without affecting performance. In Comparative Example 2, the amount of bio-bacteria added was relatively small, and water quality began to deteriorate in March and June. In Comparative Example 3, no energy balls were added, and the porosity was measured to be only 8.3%. This indicates that the bio-bacteria have a poor living environment and limited space, leading to deteriorating water quality.

[0126] In Comparative Example 4, all energy balls were replaced with far-infrared powder. The porosity of the biomass was measured to be only 7.8%, leaving less room for the bacteria to survive and deteriorating water quality. In Comparative Example 5, far-infrared powder was not used, and water quality began to deteriorate over time. In Comparative Example 6, no oxygen-generating filler was added, resulting in a decrease in bacterial growth and a certain impact on water quality, which was worse than the example.

[0127] The test data in table 5 to table 7 show that the dissolved oxygen BOD5 of the water of comparative example 7-13 in the 6th month and the concentration of nitrate are all higher than embodiment 1-5, illustrate that the biomass lacking these components is relatively poor to the long-time purification effect of fish tank water quality. This may be because the biomass stability with the addition of these components is better, and plant glue can strengthen the structural stability of biological bacteria active material within a certain period of time, and it can slowly naturally degrade, and in this process, both can discharge some compositions that are beneficial to biological bacteria growth, and can facilitate the slow disintegration of biological bacteria active material again, which is beneficial to environmental protection. Agar can absorb biological bacteria, and part of biological bacteria can enter in agar, play the effect of stabilizing bacteria, and when outside bacterium is few, the bacterium diffusion in agar enters water, and is beneficial to beneficial bacteria in long-term stable water. Bran provides biological bacteria nutrition, provides protein, vitamins and mineral substances (magnesium, zinc) etc., and the fiber of bran forms a network structure adsorbable thalline, plays stable bacteria and provides carbon and nitrogen source, and the long-term stability of biological bacteria is had an effect. Bagasse provides a source of carbon and nitrogen for the bioreactors, while the fibers form a network structure that absorbs the bacteria and stabilizes them. Yeast extract provides comprehensive nutrition, supporting microbial growth. Dihydrogen phosphate stabilizes the pH in the water, providing essential elements for bacterial growth and absorbable inorganic phosphorus.

[0128] The data in Table 7 show that the activity of the fish in Comparative Example 13 began to deteriorate in the sixth month. This is because the agar, bran, bagasse, yeast extract, and dihydrogen phosphate were omitted from Comparative Example 13. The plant fibers contained in bagasse and bran, combined with cement, volcanic mud, and medical stone powder, not only give the bioactive filler a loose and porous structure that maintains stability over a long period of time, but also allow the bioactive material to slowly release components that promote the growth of mycelium from the bioactive bacteria, thereby enhancing purification while preventing excessive spore growth. Furthermore, as previously mentioned, agar, yeast extract, and dihydrogen phosphate are effective in stabilizing the biomass, so the absence of these components reduces the long-term stability of the biomass.

[0129] In addition, tests have shown that the number of bacterial propagation holes in the bio-blocks prepared in the embodiments of the present invention reaches more than 30%, which is more conducive to the growth and reproduction of biological bacteria in water. In addition, the bio-blocks have high flexural strength and certain stability in water.

[0130] Pond water quality treatment test:

[0131] Before treatment: The pond stank, there were no fish, and the bottom of the pond turned black and foamy.

[0132] Treatment results: After the biomass prepared by the above method was placed in a pond, the pond water gradually became clear after half a month. The BOD5 continued to decrease. One month later, ornamental fish were added and the fish activity returned to normal.

[0133] In summary, experimental tests have shown that the biological bacteria active material provided by the present invention has the following advantages for water purification: 1) the aquatic ecosystem of the fish tank is well maintained and the water can be kept unchanged for a long time. By placing the bio-blocks in an ornamental fish pond, the water quality of a large ornamental fish pond can also be purified; 2) inorganic natural colorants can be added to the mixture to adjust it to a rockery color, and the plastic mold can be made into a rockery, which increases the ornamental value and can regulate the water quality; 3) for polluted rivers and sewers, the addition of bio-blocks can continuously reduce the BOD5, clear the water quality, and gradually restore the ecosystem.

[0134] In addition, the inventors of this case also referred to the aforementioned embodiments and conducted experiments using other raw materials, process operations, and process conditions described in this specification, and obtained relatively ideal results.

[0135] The above-described embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the patent for this invention shall be determined by the appended claims.

Claims

1. A biological bacterial active material, characterized in that: The raw materials of the biological bacteria active material include the following components calculated by mass percentage: 20-50% of biological active filler, 5%-20% of oxygen-generating filler, 10-30% of medical stone, 0-30% of ceramic particles, 0-30% of quartz sand, 5-20% of aluminum phosphate cement, 1-3% of water reducer, 10-30% of biological bacteria spore composition and 3-5% of plant glue; The raw materials of the bioactive filler include the following components calculated by mass percentage: 30-70% aluminum phosphate cement, 20-60% volcanic mud, 5-40% medical stone powder, 2-10% active additives, 0.5-2% agar, 2-5% bran, 3-8% bagasse, 0.1-0.5% yeast extract and 0.1-1% dihydrogen phosphate; the active additives include any one of far-infrared powder and silica fume, or a combination of two of them.

2. The biological active material according to claim 1, characterized in that: The bioactive filler is spherical particles with a particle size of 0.4 cm to 0.6 cm.

3. The biological active material according to claim 1, characterized in that: The preparation method of the bioactive filler comprises: adding aluminum phosphate cement, volcanic mud, medical stone powder, active additives, agar, bran, bagasse, yeast extract and dihydrogen phosphate into a round pot granulator and spraying water to form balls to obtain the bioactive filler; Preferably, the process conditions for water-spraying pelletizing include: the rotation speed of the round pot granulator is 10-35 r / min, the temperature of water-spraying pelletizing is room temperature, the time of water-spraying pelletizing is 20-30 min, and the amount of water sprayed is 18-35% of the mass of the dry material.

4. The biological active material according to claim 1, characterized in that The particle size of the volcanic mud is 250-400 mesh; and / or the particle size of the medical stone powder in the raw material of the bioactive filler is 250-400 mesh, and / or the particle size of the active additive is 250-400 mesh; and / or the particle size of the agar is 200-320 mesh; and / or the particle size of the yeast paste is 200-320 mesh; and / or the particle size of the bran is 200-320 mesh; and / or the particle size of the bagasse is 200-320 mesh.

5. The biological active material according to claim 1, characterized in that: The preparation method of the oxygen-generating filler comprises: uniformly mixing zeolite powder, sodium alginate and green algae suspension to obtain a mixed solution, dropping the mixed solution into a calcium chloride solution to form gel beads, and solidifying the mixed solution to obtain the oxygen-generating filler.

6. The biological active material according to claim 1, characterized in that: The particle size of medical stone in the raw material of the biological bacteria active material is 20 meshes to 60 meshes; And / or, the particle size of the ceramic particles is 20 mesh to 60 mesh; And / or, the particle size of the quartz sand is 20 mesh to 60 mesh; and / or, the water reducer comprises a polycarboxylate water reducer; and / or, the plant gum comprises sesbania gum, guar gum or fenugreek gum; And / or, the biological bacteria spore composition includes 30-60 wt% of Bacillus subtilis spores, 15-30 wt% of Bacillus thuringiensis spores, 20-40 wt% of Bacillus sphaericus spores, 5-15 wt% of yeast and 5-25 wt% of lactic acid bacteria.

7. The biological active material according to claim 1, characterized in that: The biological bacteria active material is a block material with a porosity of 30-45% and a pore diameter of 20 μm-5 mm.

8. The method for preparing the biological active material according to any one of claims 1 to 7, characterized in that: include: The bioactive filler, the oxygen-generating filler, medical stone, ceramic particles (optionally added or not added), quartz sand (optionally added or not added), aluminum phosphate cement, a biological bacteria spore composition, plant glue, a water reducer and water are mixed to obtain a mixture, and the mixture is molded to obtain the biological bacteria active material; Preferably, the mass content of water in the mixture is 8 to 15%; Preferably, the process conditions of the molding treatment include: placing the mixed material in a mold for drying and molding at room temperature.

9. Use of the biological bacterial active material according to any one of claims 1 to 7 in water purification.

10. A water purification method, characterized in that: include: Immerse the biological bacteria active material according to any one of claims 1 to 7 in the water to be purified.

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