Breeding method of cynoglossus brevitarsis

Abstract

The invention relates to the technical field of fish culture, in particular to a culture method of cynoglossus brevitarsis. A breeding method of cynoglossus brevitarsis comprises the following steps: S1, adopting a runway type breeding box for live-keeping breeding: putting wild cynoglossus brevitarsis caught in sea into prepared disinfected seawater for temporary breeding for more than 4 hours, transferring into the runway type breeding box, controlling the water temperature of the runway type breeding box to be 14-15 DEG C, and breeding cynoglossus brevitarsis in the runway type breeding box; after the cynoglossus brevis transferred into the runway type breeding box is temporarily bred for three days, starting to feed the cynoglossus brevis with keep-alive feed; s2, carrying out gonad maturation promotion on the wild cynoglossus brevitarsis; and S3, carrying out spawning induction on the wild cynoglossus brevitarsis. Compared with the prior art, the breeding method has the advantages that in the breeding method of the cynoglossus brevitarsis, the unique runway type breeding box is combined with the flowing water baffle design, double-flow-state control of upper-layer stable water fish breeding and lower-layer high-speed dirt flushing is achieved, the problem of body rot caused by accumulation of residual feed in a traditional still water pool is thoroughly solved, and the survival rate within 7 days is increased to 93.2% from 41.7%.

Description

Technical Field

[0001] The invention relates to the technical field of fish breeding, and in particular to a breeding method for shortsnout three-line tongue sole. Background Art

[0002] The short-snout three-line tongue sole (Cynoglossus abbreviatus) belongs to the order Pleuronectiiformes, family Cynoglossidae, genus Cynoglossus. It is distributed in the tropical and subtropical waters of the western Pacific and Indian Oceans. In my country, it is primarily found in the southern East China Sea, the Taiwan Strait, and the South China Sea, with a few species also found in freshwater areas. It is a warm-temperate, benthic fish of coastal and estuary habitats, often inhabiting sandy bottoms or within the sand. Its meat is delicious, firm, delicate, and boneless, and it has high medicinal value, known for its tonifying, spleen-strengthening, and qi-boosting properties. It has a market value of over 200 yuan per kilogram. Currently, the short-snout three-line tongue sole on the market is primarily captured.

[0003] Short-snout three-line tongue sole ( Cynoglossus abbreviatus ( ) belongs to the order Pleuronectiformes, family Glossopteriidae, genus Glossopteris. It is a warm-temperate, benthic fish found along coasts and in estuaries in the tropical and subtropical waters of the western Pacific and Indian Oceans. Its flesh is delicious, firm, delicate, and boneless, and boasts benefits such as tonifying the body, strengthening the spleen, and invigorating Qi. With a market value of over 200 yuan per kilogram, it is highly sought after by consumers. However, in recent years, due to environmental degradation and overfishing, the wild population of the short-snout three-line tongue sole has plummeted, raising concerns about species conservation and artificial breeding. Currently, research on the artificial breeding and aquaculture of the short-snout three-line tongue sole remains elusive. Traditional aquaculture methods present numerous challenges, such as body rot caused by residual bait accumulation and low survival rates. In particular, traditional still-water tanks are prone to residual bait accumulation, which compromises fish health and reduces aquaculture efficiency. Furthermore, existing aquaculture systems often suffer from difficult water quality management, high energy consumption, and high maintenance costs, making them difficult to scale up and implement. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to provide a breeding method of short-snout three-line tongue sole with high breeding efficiency.

[0005] In order to solve the above technical problems, the technical solution adopted by the present invention is: to provide a method for cultivating short-snout three-line tongue sole, comprising the following steps: S1. Use raceway-type breeding boxes to keep alive: The wild short-snout three-line tongue soles caught at sea were placed in prepared sterilized seawater for temporary culture for more than 4 hours, and then transferred to a raceway-type culture box. The water temperature of the raceway-type culture box was controlled at 14°C-15°C. After three days of temporary culture, the short-snout three-line tongue soles transferred to the raceway-type culture box were fed with a survival feed. The short-snout three-line tongue soles were checked for death every day, and the dead ones were promptly picked out; The racetrack-type aquaculture box comprises two parallel straight lanes and two curved lanes, wherein the straight lanes are provided with a water flow baffle with a water-passing gap at the bottom; the curved lanes are provided with a water push pump for circulating aquaculture seawater in the aquaculture box; S2. Gonadal maturation of wild short-snout three-line tongue sole: When the shortnose three-line tongue soles temporarily cultured in step S1 reach an average weight of 400 grams for females and 100 grams for males, the water temperature is raised to 19°C by increasing the water temperature by 1°C every three days, and gonadotropin-stimulating feed is started; S3. Induced spawning of wild short-snout three-line tongue sole: After the water temperature rises to 19°C and gonad development feed is fed for 15 days, when the female fish's gonads are obviously swollen, the water flow baffle of the raceway-type breeding box is removed, and a layer of fine sand is added to the straight bottom of the raceway-type breeding box. The female fish and male fish are mixed in a ratio of 1:2. No more feeding is done. After 46 hours, the amount of eggs in the water is observed, the fertilized eggs are collected, and placed in a conical bucket for incubation.

[0006] The beneficial effects of the present invention are as follows: the innovative raceway-style culture box combined with a water flow baffle design in the culture method of short-snout three-line tongue sole achieves dual flow control of "upper layer stable water for fish cultivation / lower layer high-speed sewage flushing", completely solving the problem of body rot caused by residual bait accumulation in traditional static water ponds, and the 7-day survival rate jumps from 41.7% to 93.2%; Maturation-promoting stage: A hormone-free feed formula utilizes the physiological synergistic effect of nereid ecdysterone and oyster zinc, combined with a gradient temperature increase process, shortening the gonadal maturation cycle from the natural state of 8-10 months to about 1 month, and there is no risk of drug residues); Natural spawning: a fine sand layer is laid to simulate the benthic spawning environment, combined with a single pump water flow stimulation to induce natural spawning behavior, increase the spawning rate, and completely avoid hormone residues; Resource utilization rate: The water flow baffle water channel design improves the water recycling rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0007] Figure 1 This is a schematic structural diagram of a raceway-type breeding box according to a specific embodiment of the present invention; Description of labels: 1. Straight road; 2. Curve; 3. Water flow baffle; 4. Drainage outlet; 5. Water inlet; 6. Short-snout three-line tongue sole. DETAILED DESCRIPTION

[0008] To illustrate the technical content, achieved objectives and effects of the present invention in detail, the following description is given in conjunction with the embodiments and accompanying drawings.

[0009] The present invention provides a method for cultivating short-snout three-line tongue sole, comprising the following steps: S1. Use raceway-type breeding boxes to keep alive: The wild short-snout three-line tongue soles caught at sea were placed in prepared sterilized seawater for temporary culture for more than 4 hours, and then transferred to a raceway-type culture box. The water temperature of the raceway-type culture box was controlled at 14°C-15°C. After three days of temporary culture, the short-snout three-line tongue soles transferred to the raceway-type culture box were fed with a survival feed. The short-snout three-line tongue soles were checked for death every day, and the dead ones were promptly picked out; The racetrack-type aquaculture box comprises two parallel straight lanes and two curved lanes, wherein the straight lanes are provided with a water flow baffle with a water-passing gap at the bottom; the curved lanes are provided with a water push pump for circulating aquaculture seawater in the aquaculture box; S2. Gonadal maturation of wild short-snout three-line tongue sole: When the shortnose three-line tongue soles temporarily cultured in step S1 reach an average weight of 400 grams for females and 100 grams for males, the water temperature is raised to 19°C by increasing the water temperature by 1°C every three days, and gonadotropin-stimulating feed is started; S3. Induced spawning of wild short-snout three-line tongue sole: After the water temperature rises to 19°C and gonad development feed is fed for 15 days, when the female fish's gonads are obviously swollen, the water flow baffle of the raceway-type breeding box is removed, and a layer of fine sand is added to the straight bottom of the raceway-type breeding box. The female fish and male fish are mixed in a ratio of 1:2. No more feeding is done. After 46 hours, the amount of eggs in the water is observed, the fertilized eggs are collected, and placed in a conical bucket for incubation.

[0010] The beneficial effects of the present invention are as follows: the innovative raceway-style culture box combined with a water flow baffle design in the culture method of short-snout three-line tongue sole achieves dual flow control of "upper layer stable water for fish cultivation / lower layer high-speed sewage flushing", completely solving the problem of body rot caused by residual bait accumulation in traditional static water ponds, and the 7-day survival rate jumps from 41.7% to 93.2%; Maturation-promoting stage: A hormone-free feed formula utilizes the physiological synergistic effect of nereid ecdysterone and oyster zinc, combined with a gradient temperature increase process, shortening the gonadal maturation cycle from the natural state of 8-10 months to about 1 month, and there is no risk of drug residues); Natural spawning: a fine sand layer is laid to simulate the benthic spawning environment, combined with a single pump water flow stimulation to induce natural spawning behavior, increase the spawning rate, and completely avoid hormone residues; Resource utilization rate: The water flow baffle water channel design improves the water recycling rate.

[0011] Furthermore, in the above-mentioned method for cultivating short-snout three-line tongue sole, a drainage outlet and a water inlet are provided on the bend.

[0012] Furthermore, in the above-mentioned method for cultivating short-snout three-line tongue sole, in step S3, the fertilized eggs are collected through the drainage outlet using a sieve mesh.

[0013] Furthermore, in the above-mentioned breeding method of short-snout three-line tongue sole, the drainage outlet of the racetrack-type breeding box is arranged at the point with the maximum curvature on the curve.

[0014] As can be seen from the above description, the above settings optimize the water flow dynamics and prevent the accumulation of residual bait.

[0015] Furthermore, in the above-mentioned breeding method of short-snout three-line tongue sole, the survival feed is made of the following raw materials in parts by weight: 35 parts of fish meal, 25 parts of soybean meal, 15 parts of corn gluten meal, 3 parts of squid viscera powder, 4 parts of fish oil, 3 parts of soybean lecithin, 2 parts of calcium dihydrogen phosphate, 1.5 parts of multivitamin premix, 1.5 parts of multimineral premix, 5 parts of sargassum powder, 2 parts of sodium carboxymethyl cellulose and 1 part of gum arabic.

[0016] Furthermore, in the above-mentioned breeding method of short-snout three-line tongue sole, the gonad maturation-promoting feed is made of the following raw materials in parts by weight: 35 parts of fish meal, 15 parts of double-toothed sandworm dry powder, 0.5 parts of vitamin E, 14.5 parts of corn protein meal, 18 parts of Omi oyster dry powder, 4 parts of fish oil, 3 parts of soybean lecithin, 2 parts of calcium dihydrogen phosphate, 1.5 parts of multivitamin premix, 1.5 parts of multimineral premix, 5 parts of sargassum powder and 3 parts of gum arabic.

[0017] Furthermore, in the above-mentioned method for cultivating short-snout three-line tongue sole, the method for preparing the sterilized seawater is: sterile seawater with a salinity of 20 is prepared using sterilized sea salt and ultrapure water, and 3 mg / L of penicillin and 2 mg / L of streptomycin are added to prepare the sterilized seawater.

[0018] Furthermore, in the above-mentioned breeding method of short-snout three-line tongue sole, the breeding seawater is prepared by the following method: ordinary seawater is first filtered through a sand filter and then enters a reservoir, the reservoir is connected to a pipeline ultraviolet sterilizer and an ozone generator, and is circulated and sterilized for 4 hours and then aerated for 10 hours, and then pumped into the breeding pond by a water pump for use as breeding seawater.

[0019] Example 1 Please refer to Figure 1 A method for cultivating short-snout three-line tongue sole comprises the following steps: Step 1: Preparation for breeding of short-snout three-line tongue sole: Step 101, preparing sterilized seawater: ultrapure water and salt are mixed to prepare sterile seawater with a salinity of 20, and 3 mg / L of penicillin and 2 mg / L of streptomycin are added to prepare sterilized seawater; Step 102, aquaculture seawater treatment: Ordinary seawater is filtered through a 200-mesh sand filter and then enters a water reservoir. The water reservoir is connected to a pipeline-type ultraviolet sterilizer and an ozone generator. After a 4-hour cycle sterilization, it is aerated in an aeration tower for 10 hours and then pumped into a raceway-type aquaculture box for use as aquaculture seawater. Step 103: Bait preparation: The survival feed was prepared by weighing the following raw materials in parts by weight: 35 kg of fish meal, 25 kg of soybean meal, 15 kg of corn gluten meal, 3 kg of squid viscera meal, 4 kg of fish oil, 3 kg of soy lecithin, 2 kg of calcium dihydrogen phosphate, 1.5 kg of multivitamin premix, 1.5 kg of multimineral premix, 5 kg of sargassum powder, 2 kg of sodium carboxymethyl cellulose, and 1 kg of gum arabic. The gonad-promoting feed is made by weighing the raw materials according to the following weight parts: 35kg of fish meal, 15kg of double-toothed sandworm dry powder, 0.5kg of vitamin E, 14.5kg of corn protein powder, 18kg of Omi oyster dry powder, 4kg of fish oil, 3kg of soybean lecithin, 2kg of calcium dihydrogen phosphate, 1.5kg of multivitamin premix, 1.5kg of multimineral premix, 5kg of sargassum powder, and 3kg of gum arabic.

[0020] Feed molds: 3D-printed shrimp-shaped molds are used for feed production. Different sizes of shrimp-shaped feed molds are produced based on the size of the live tongue sole. The feed is prepared using the above feed formula, softened by adding 20% water, pressed into shape using a mold, and air-dried before feeding.

[0021] Step 2: Breeding of short-snout three-line tongue sole: Step 201: placing the captured wild short-snout tongue sole in sterilized seawater for temporary culture for 4-6 hours, and then transferring the captured short-snout tongue sole to a raceway-type culture box, wherein the water temperature of the raceway-type culture box is controlled at 14-15° C.; after three days of temporary culture, the short-snout tongue sole 6 transferred to the raceway-type culture box is fed with a keep-alive feed, and the short-snout tongue sole 6 is checked for death every day, and any dead ones are promptly removed; The racetrack-type aquaculture box comprises two parallel straight lanes 1 and two curved lanes 2. The straight lane 1 is provided with a water flow baffle 3 with a water-passing gap at the bottom; the curved lane 2 is provided with a water push pump for circulating aquaculture seawater in the aquaculture box; the curved lane is provided with a drain port 4 and a water inlet 5.

[0022] Step 202: Place the captured triglossus brevis in the water baffle 3 at the straight end of the runway, ensuring an even distribution of the triglossus brevis and avoiding crowding or overlap. This allows the bait to move along the runway, creating a feeding attraction. Both bends of the runway are equipped with water pumps to create circulating water within the runway, cleaning the runway and moving the bait within the runway, creating a feeding attraction.

[0023] Turn on a water pump at each circular end of the raceway to create a flowing water flow within the raceway for oxygenation. When feeding is needed, turn on both push pumps simultaneously to increase the water flow. Add the preservative feed prepared according to the above recipe to the front of the linear raceway, driving the shrimp-shaped feed along the bottom of the raceway and enticing the shortnose sole to feed. Once the sole has finished feeding, open the drain and water inlet to remove any remaining bait and feed from the raceway.

[0024] Step 203: When the average weight of the short-snout three-line tongue sole reaches 400-450 grams for female fish and 100-120 grams for male fish, the water temperature is raised to 18-20° C. by increasing the water temperature by 1° C. every 3 days, and the gonad-promoting feed is started to be fed; Step 204: After the water temperature rises to 19°C and the gonad development feed is fed for 15 days, when the gonads of the female fish are obviously swollen, the water flow baffle is completely removed, and a fine sand layer with a thickness of 1-2 cm is evenly added to the straight bottom surface of the breeding box to prepare the incubation device.

[0025] Step 3: Induced spawning of short-snout three-line tongue sole: Step 301: Mix the female fish and the male fish in a ratio of 1:2 and do not feed them any more bait; Step 302: Collect the fertilized eggs through a silk screen set at the drain outlet; and place them in a conical bucket for incubation.

[0026] The drain outlet of the raceway-type breeding box is set at the maximum curvature on the curve, which prevents the accumulation of residual bait by optimizing water flow dynamics. During the breeding process, water quality parameters are regularly tested.

[0027] The above descriptions are merely embodiments of the present invention and are not intended to limit the patent scope of the present invention. Any equivalent transformations made using the contents of the present invention's description and drawings, or directly or indirectly applied in related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A method for cultivating short-snout three-line tongue sole, characterized in that: The steps include: S1. Use raceway-type breeding boxes to keep alive: The wild short-snout three-line tongue soles caught at sea were placed in prepared sterilized seawater for temporary culture for more than 4 hours, and then transferred to a raceway-type culture box. The water temperature of the raceway-type culture box was controlled at 14°C-15°C. After three days of temporary culture, the short-snout three-line tongue soles transferred to the raceway-type culture box were fed with a survival feed. The short-snout three-line tongue soles were checked for death every day, and the dead ones were promptly picked out; The racetrack-type aquaculture box comprises two parallel straight lanes and two curved lanes, wherein the straight lanes are provided with a water flow baffle with a water-passing gap at the bottom; the curved lanes are provided with a water push pump for circulating aquaculture seawater in the aquaculture box; S2. Gonadal maturation of wild short-snout three-line tongue sole: When the shortnose three-line tongue soles temporarily cultured in step S1 reach an average weight of 400 grams for females and 100 grams for males, the water temperature is raised to 19°C by increasing the water temperature by 1°C every three days, and gonadotropin-stimulating feed is started; S3. Induced spawning of wild short-snout three-line tongue sole: After the water temperature rises to 19°C and gonad development feed is fed for 15 days, when the female fish's gonads are obviously swollen, the water flow baffle of the raceway-type breeding box is removed, and a layer of fine sand is added to the straight bottom of the raceway-type breeding box. The female fish and male fish are mixed in a ratio of 1:

2. No more feeding is done. After 46 hours, the amount of eggs in the water is observed, the fertilized eggs are collected, and placed in a conical bucket for incubation.

2. The method for cultivating the short-snout three-line tongue sole according to claim 1, wherein: A drain outlet and a water inlet are provided on the bend.

3. The method for cultivating the short-snout three-line tongue sole according to claim 1, wherein: In step S3, the fertilized eggs are collected through the drain port using a sieve.

4. The method for cultivating the short-snout three-line tongue sole according to claim 1, wherein: The drainage outlet of the raceway-type breeding box is arranged at the point where the curvature is maximum on the curve.

5. The method for cultivating the short-snout three-line tongue sole according to claim 1, wherein: The survival feed comprises the following raw materials in parts by weight: 35 parts of fish meal, 25 parts of soybean meal, 15 parts of corn gluten meal, 3 parts of squid viscera powder, 4 parts of fish oil, 3 parts of soybean lecithin, 2 parts of calcium dihydrogen phosphate, 1.5 parts of multivitamin premix, 1.5 parts of multimineral premix, 5 parts of sargassum powder, 2 parts of sodium carboxymethyl cellulose and 1 part of gum arabic.

6. The method for cultivating the short-snout three-line tongue sole according to claim 1, wherein: The gonad maturation-promoting feed is made of the following raw materials in parts by weight: 35 parts of fish meal, 15 parts of double-toothed sandworm dry powder, 0.5 parts of vitamin E, 14.5 parts of corn gluten meal, 18 parts of Omi oyster dry powder, 4 parts of fish oil, 3 parts of soybean lecithin, 2 parts of calcium dihydrogen phosphate, 1.5 parts of complex vitamin premix, 1.5 parts of complex mineral premix, 5 parts of sargassum powder and 3 parts of gum arabic.

7. The method for cultivating the short-snout three-line tongue sole according to claim 1, wherein: The preparation method of the sterilized seawater is as follows: sterilized seawater with a salinity of 20 is prepared by using sterilized sea salt and ultrapure water, and 3 mg / L of penicillin and 2 mg / L of streptomycin are added to prepare the sterilized seawater.

8. The method for cultivating the short-snout three-line tongue sole according to claim 1, wherein: The aquaculture seawater is prepared by the following method: ordinary seawater is first filtered through a sand filter and then enters a water reservoir, the water reservoir is connected to a pipeline-type ultraviolet sterilizer and an ozone generator, and is circulated and sterilized for 4 hours and then aerated for 10 hours, and then pumped into the aquaculture pond by a water pump for use as aquaculture seawater.

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