Bacterial strain cultivation device for chymosin preparation

Abstract

The invention relates to the technical field of strain cultivation equipment, in particular to a strain cultivation device for chymosin preparation, which comprises a shaking table and an adaptive bearing device mounted at the movable end of the shaking table, the adaptive bearing device comprises a folding adjusting frame fixedly mounted at the movable end of the shaking table, and the folding adjusting frame comprises a plurality of mounting plates; lifting push plates are arranged between the adjacent mounting plates, the lifting push plates are fixedly connected with the bottoms of the mounting plates, the lifting push plates and the mounting plates are connected in series through guide columns, and cross lifting devices are mounted between the lifting push plates and the mounting plates and used for adjusting the distance between the lifting push plates and the mounting plates in a lifting mode. Detachable bearing plates are mounted on the mounting plates, the bearing plates are used for bearing the culture tanks, a separation buffer mechanism is mounted on the outer side of each mounting plate, and the outer sides of the separation buffer mechanisms abut against the inner wall of the shaking table. The container bearing capacity and adaptability are effectively improved, the replacement process is simplified, and efficient culture is guaranteed.

Description

Technical Field

[0001] The invention relates to the technical field of strain cultivation equipment, in particular to a strain cultivation device for preparing rennet. Background Art

[0002] Rennet plays a vital role in cheesemaking. Traditionally, rennet production relies primarily on animal sources (such as calf stomach extraction). However, due to limited raw material supply and ethical concerns, microbial fermentation has gradually become the mainstream. Currently, microbial rennet production typically utilizes liquid submerged fermentation technology.

[0003] Chinese patent number CN222411526U discloses a shaker for strain cultivation, comprising: a rectangular box, a rocking plate movably connected to the bottom of the inner cavity of the rectangular box, a fixed clamp fixedly connected to the top of the rocking plate, and four fixed clamps of the same size; a clamping mechanism, the clamping mechanism being arranged on the front of the rocking plate; wherein the clamping mechanism includes a pneumatic cylinder. The device is provided with a pneumatic cylinder, a rectangular block, a connecting rod, and a movable clamp. When the pneumatic cylinder is in operation, it will drive the rectangular block to move upward, thereby causing the two connecting rods to rotate and drive the two movable clamps to move away from each other. With the cooperation of the two movable clamps and the four fixed clamps, multiple culture containers can be clamped and fixed simultaneously, solving the problem of relying on manual fixing one by one, and improving the convenience of the device.

[0004] The above-mentioned device still has significant technical defects in practical application. First, during the replacement of culture containers, the staff must take out multiple culture containers from the shaking plate in turn each time the culture containers are replaced. The operation process is cumbersome, resulting in a slow replacement speed, which greatly affects the continuity and efficiency of strain cultivation. Second, the existing device has limitations in the number of culture containers it can carry, and it is difficult to adapt to the demand for synchronous operation of a large number of culture containers during large-scale strain cultivation. The above-mentioned problems directly restrict the efficiency of strain cultivation, thereby affecting the production process of rennet and reducing the overall production efficiency. Summary of the Invention

[0005] In response to the above problems, a strain cultivation device for rennet preparation is provided, which effectively improves the container's carrying capacity and adaptability through an adaptive supporting device, simplifies the replacement process, and ensures efficient cultivation.

[0006] In order to solve the problems of the prior art, the present invention provides a strain cultivation device for preparing rennet, comprising a shaker and an adaptive supporting device installed at the movable end of the shaker, the adaptive supporting device comprising a folding adjustment frame fixedly installed at the movable end of the shaker, the folding adjustment frame comprising a plurality of mounting plates, a lifting push plate being provided between adjacent mounting plates, the lifting push plate being fixedly connected to the bottom of the mounting plate, the lifting push plate and the mounting plate being connected in series through a guide column, the lifting push plate and the mounting plate being slidably connected to the guide column, a cross-lifting device being installed between the lifting push plate and the mounting plate, the cross-lifting device being used to lift and adjust the distance between the lifting push plate and the mounting plate, a detachable supporting plate being installed on the mounting plate, the supporting plate being used to support a culture tank, a partitioning buffer mechanism being installed on the outer side of each mounting plate, the outer side of the partitioning buffer mechanism being in contact with the inner wall of the shaker.

[0007] Preferably, two first limiting slide rails are installed on the mounting plate, and a quick-release clamping device is also installed on the mounting plate. The quick-release clamping device includes a guide mounting rail, and sliding clips are slidably installed on both sides of the guide mounting rail. The side of the sliding clip is provided with a guide socket, and a first spring is installed between each sliding clip and the guide mounting rail. A push-unlocking block is also slidably installed on the guide mounting rail, and a V-shaped unlocking groove is provided at one end of the push-unlocking block, and the V-shaped unlocking groove is slidably docked with the guide socket.

[0008] Preferably, the cross lifting device includes two bidirectional adjustment screw slides distributed inside the first limit slide rail, the two bidirectional adjustment screw slides are synchronously driven by a gear transmission assembly, and two pushing links are rotatably installed on the movable end of each bidirectional adjustment screw slide, the two pushing links are cross-arranged and rotatably connected to each other, and a sliding push block is installed at the end of the pushing link away from the bidirectional adjustment screw slide, the sliding push block is slidably connected to the lifting push plate, and anti-slip teeth are provided on the side of the sliding push block. The cross lifting device also includes a cross positioning device installed between the mounting plate and the lifting push plate.

[0009] Preferably, the cross-positioning device includes two cross-arranged hydraulic positioning rods, the hydraulic positioning rod includes a rotating rod rotatably mounted on a mounting plate, a suction chamber is provided inside the rotating rod, a telescopic rod is slidably mounted on the rotating rod, a suction piston is provided at one end of the telescopic rod, the suction piston is arranged inside the suction chamber, and the end of the telescopic rod away from the suction piston is rotatably connected to the lifting push plate. The cross-positioning device is also provided with an oil storage tank, hydraulic oil is provided inside the oil storage tank, the output end of the oil storage tank is connected to the suction chamber, and a control valve is installed at the output end of the oil storage tank.

[0010] Preferably, two second limit slide rails are provided at the bottom of the lifting push plate, the second limit slide rails are used to connect the sliding push block, and the side of the second limit slide rail is provided with a limit clamping mechanism, the limit clamping mechanism includes a limit clamping strip installed on the side of the second limit slide rail, the side of the limit clamping strip is provided with a plurality of limit teeth, the limit teeth and the anti-slip teeth are clamped with each other, a second spring is installed between the limit clamping strip and the second limit slide rail, an extrusion push block is installed between the second limit slide rail and the limit clamping strip, the extrusion push block is slidably connected to the limit clamping strip, and a separation protrusion is provided on the extrusion push block.

[0011] Preferably, a limit mounting groove is provided at the bottom of the support plate, and two limit card holes are provided on the inner side of the limit mounting groove, and the limit card holes are connected to the sliding card strip. A plurality of limit clamping devices are installed on the support plate, and the limit clamping devices are used to clamp the culture tank. An adsorption layer is also installed on the support plate, and the adsorption layer is arranged above the limit clamping device, and a plurality of limit placement holes are provided on the adsorption layer.

[0012] Preferably, the limit clamping device includes a plurality of fixed clamping blocks fixedly mounted on the support plate, a movable clamping block is mounted on the side of each fixed clamping block, and a plurality of V-shaped clamping grooves are provided on the fixed clamping block and the movable clamping block. The limit clamping device also includes a pushing rod slidably mounted on the support plate, a connecting push block is slidably mounted on the pushing rod, a third spring is installed between the connecting push block and the pushing rod, synchronous push rods are rotatably mounted on both sides of the connecting push block, and the synchronous push rod is rotatably connected to the movable clamping block at one end away from the connecting push block.

[0013] Preferably, the separation and buffering mechanism includes a fixed frame installed on the side of the mounting plate, a pressing frame is sleeved on the outer side of the fixed frame, a plurality of fourth springs are installed between the fixed frame and the pressing frame, and a flexible partition is installed on the fixed frame and the pressing frame.

[0014] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention provides a folding adjustment frame, which includes multiple mounting plates, and a lifting push plate is provided between adjacent mounting plates. The lifting push plate and the mounting plate are slidably connected by a guide column and are equipped with a cross-lifting device. The staff operates the cross-lifting device to drive the lifting push plate to rise or retract, thereby adjusting the distance between the lifting push plate and the mounting plate. When the lifting push plate rises, it provides more mounting positions for multiple support plates, greatly increasing the number of culture containers that can be carried, and can meet the demand for synchronous operation of a large number of culture containers during large-scale strain cultivation; when the lifting push plate retracts, the top mounting plate can obtain sufficient space to adapt to the cultivation requirements of larger-sized culture tanks, significantly improving the adaptability of the device to culture tanks of different specifications, effectively solving the limitations of existing devices in terms of the number of culture containers that can be carried and the adaptability of specifications, and providing better hardware support for strain cultivation in the rennet preparation process.

[0015] 2. The mounting plate of the present invention is used in conjunction with a detachable support plate. When the bacterial liquid culture is completed, the staff can directly remove the support plate from the mounting plate, and then quickly install the support plate with the new culture tank. This design changes the cumbersome operating process of the traditional device in which multiple culture containers need to be taken out from the shaking plate in sequence each time the culture container is replaced, effectively reducing the time for replacing the culture tank. At the same time, the separation buffer mechanism is arranged between the shaker and the mounting plate, which not only effectively reduces the swing force of the lifting push plate and the mounting plate, but also the guide column and the cross lifting device provide support, jointly ensuring the strength and stability of the adaptive support device when it is shaken as a whole, avoiding damage to the adaptive support device structure or displacement of the culture tank due to shaking, ensuring the continuity and efficiency of the strain cultivation, and thus improving the overall production efficiency of the rennet production process based on the cultivation of this strain. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 The present invention is a three-dimensional schematic diagram of a bacterial strain cultivation device for preparing chymosin.

[0017] Figure 2 The present invention is a front view of a bacterial strain cultivation device for preparing chymosin.

[0018] Figure 3 It is a three-dimensional schematic diagram of an adaptive supporting device in a bacterial strain cultivation device for preparing chymosin according to the present invention.

[0019] Figure 4 It is a right view of an adaptive supporting device in a bacterial strain cultivation device for preparing chymosin according to the present invention.

[0020] Figure 5 The present invention is a three-dimensional schematic diagram of a folding adjustment frame in a strain cultivation device for preparing chymosin.

[0021] Figure 6 The present invention is a front view of a mounting plate in a bacterial strain cultivation device for preparing chymosin.

[0022] Figure 7 yes Figure 6 Plane sectional view at section AA.

[0023] Figure 8 The present invention is a three-dimensional schematic diagram of a lifting and pushing plate in a bacterial strain cultivation device for preparing chymosin.

[0024] Figure 9 The present invention is a three-dimensional schematic diagram of a support plate in a bacterial strain cultivation device for preparing chymosin.

[0025] Figure 10 The present invention is an exploded view of a support plate in a bacterial strain cultivation device for preparing chymosin.

[0026] Figure 11 yes Figure 10 A partial enlarged view of point B in the middle.

[0027] Figure 12 The present invention is a stereoscopic schematic diagram of a separation and buffering mechanism in a strain cultivation device for preparing chymosin.

[0028] The numbers in the figure are: 1. Rocking table; 2. Folding adjustment frame; 21. Mounting plate; 211. First limit rail; 212. Quick release clamping device; 213. Guide mounting rail; 214. Sliding clamping strip; 2141. Guide socket; 215. First spring; 216. Push unlocking block; 217. V-shaped unlocking groove; 22. Cross lifting device; 221. Bidirectional adjustment screw slide; 222. Gear transmission assembly; 223. Push connecting rod; 224. Sliding push block; 225. Cross positioning device; 2251. Control valve; 2252. Rotating rod; 2253. Telescopic rod; 2254. Oil storage tank; 23. Lifting Push plate; 231, second limiting slide rail; 2321, limiting card strip; 2322, second spring; 233, extrusion push block; 24, guide column; 3, support plate; 31, limiting mounting groove; 33, limiting clamping device; 331, fixed clamping block; 332, movable clamping block; 3321, V-shaped clamping groove; 333, pushing rod; 334, connecting push block; 335, third spring; 336, synchronous push rod; 34, adsorption layer; 35, limiting placement hole; 4, separation buffer mechanism; 41, fixed frame; 42, pressing frame; 43, fourth spring; 44, flexible partition; 5, culture tank. DETAILED DESCRIPTION

[0029] In order to further understand the features, technical means, specific objectives and functions achieved by the present invention, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0030] See also Figures 1 to 12As shown, a strain cultivation device for preparing rennet includes a shaking table 1 and an adaptive supporting device installed at the movable end of the shaking table 1, the adaptive supporting device includes a folding adjustment frame 2 fixedly installed at the movable end of the shaking table 1, the folding adjustment frame 2 includes a plurality of mounting plates 21, and a lifting push plate 23 is provided between adjacent mounting plates 21, the lifting push plate 23 is fixedly connected to the bottom of the mounting plate 21, the lifting push plate 23 and the mounting plate 21 are connected in series through a guide column 24, the lifting push plate 23 and the mounting plate 21 are both slidably connected to the guide column 24, a cross lifting device 22 is installed between the lifting push plate 23 and the mounting plate 21, the cross lifting device 22 is used to lift and adjust the distance between the lifting push plate 23 and the mounting plate 21, a detachable supporting plate 3 is installed on the mounting plate 21, the supporting plate 3 is used to support the culture tank 5, and a separation buffer mechanism 4 is installed on the outer side of each mounting plate 21, and the outer side of the separation buffer mechanism 4 is in contact with the inner wall of the shaking table 1.

[0031] During the rennet production process, the culture liquid to be cultivated is first injected into the culture tank 5, and then multiple culture tanks 5 are sequentially placed on multiple support plates 3. The support plates 3 serve as a position limit for the culture tanks 5, ensuring the initial position stability of the culture tanks 5 during the cultivation process.

[0032] When the adaptive support device cooperates with the shaking table 1 to work normally, the lifting push plate 23 and the mounting plate 21 are in a folded state with each other. In the folded state, the stability during daily shaking can be ensured and the frequency of failure can be reduced. At this time, only the top mounting plate 21 can be installed with the support plate 3 to fix the culture tank 5. When there are many culture tanks 5 to be cultivated, in order to improve the cultivation efficiency, the staff operates the cross-lifting device 22 to drive the lifting push plate 23 to rise. During the rising process of the lifting push plate 23, it moves away from the mounting plate 21, so that the lifting push plate 23 and the mounting plate 21 are spread out with each other, thereby providing more installation positions for multiple support plates 3. By increasing the number of support plates 3 to fix more culture tanks 5, the cultivation efficiency can be effectively improved.

[0033] When encountering a larger-sized culture tank 5, the staff adjusts the distance between the lifting push plate 23 and the mounting plate 21 by contraction, so that the top mounting plate 21 has enough space to adapt to the cultivation requirements of the larger-sized culture tank 5, thereby improving the adaptability of the device to the culture tank 5.

[0034] As mounting plate 21 is adjusted, it simultaneously raises and lowers the partitioning and buffering mechanism 4. This effectively divides the incubation space within shaker 1, creating multiple independent incubation zones. By coordinating with shaker 1's temperature control system, the temperature in each zone can be independently controlled, enabling simultaneous simulation of diverse environmental conditions for bacterial culture, further enhancing incubation adaptability.

[0035] When the culture tank 5 needs to be cultured, multiple support plates 3 are directly inserted into the mounting plate 21, and the mounting plate 21 limits and fixes the support plates 3. After the support plates 3 are installed in place, they further fix the culture tank 5 and ensure the installation stability of the culture tank 5 during operation.

[0036] After the shaker 1 is adjusted to an appropriate internal temperature, ensuring a suitable environment in each incubation space, the adaptive support device is then shaken. During this shaking process, the bacterial liquid in the culture tanks 5 is shaken synchronously, promoting adequate contact and even distribution of the bacterial liquid and nutrients. A partitioning and buffering mechanism 4 is positioned between the shaker 1 and the mounting plate 21, effectively reducing the rocking force of the lift push plate 23 and mounting plate 21. Simultaneously, the guide posts 24 and cross lift mechanism 22 provide support, ensuring the strength and stability of the adaptive support device during shaking, preventing structural damage or displacement of the culture tanks 5 due to shaking.

[0037] When the bacterial liquid culture is completed, the staff can directly remove the support plate 3 from the mounting plate 21 and then quickly install the support plate 3 with the new culture tank 5. This design effectively reduces the time for replacing the culture tank 5, improves the overall work efficiency, and ensures the continuity and efficiency of strain cultivation.

[0038] See also Figures 1 to 7 As shown, two first limiting slide rails 211 are installed on the mounting plate 21, and a quick-release clamping device 212 is also installed on the mounting plate 21. The quick-release clamping device 212 includes a guide mounting rail 213, and sliding clamping strips 214 are slidably installed on both sides of the guide mounting rail 213. The side of the sliding clamping strip 214 is provided with a guide socket 2141, and a first spring 215 is installed between each sliding clamping strip 214 and the guide mounting rail 213. A push-unlocking block 216 is also slidably installed on the guide mounting rail 213, and a V-shaped unlocking groove 217 is provided at one end of the push-unlocking block 216, and the V-shaped unlocking groove 217 is slidably docked with the guide socket 2141.

[0039] The first limiting slide rail 211 provides a stable installation position for the cross lifting device 22, ensuring that the cross lifting device 22 remains stable during operation, thereby ensuring the accuracy of the lifting adjustment.

[0040] The quick-release clamping device 212 equipped on the mounting plate 21 is used to quickly fix the support plate 3. The guide mounting rail 213 serves as the track for the sliding installation of the support plate 3. When the support plate 3 is slidably installed along the guide mounting rail 213, it will squeeze the sliding clamping strip 214, causing the squeezed sliding clamping strip 214 to shrink into the inside of the guide mounting rail 213. When the support plate 3 is installed to the specified position, the first spring 215 pushes the sliding clamping strip 214 to move outward and snap into the specified position of the support plate 3, thereby achieving the positioning and fixation of the support plate 3. When the support plate 3 needs to be removed, the staff presses the push unlocking block 216. The push unlocking block 216 pushes the guide socket 2141 through the V-shaped unlocking groove 217. The guide socket 2141 drives the sliding clamping strip 214 to shrink synchronously, causing the sliding clamping strip 214 to detach from the support plate 3. At this time, the support plate 3 can be removed from the guide mounting rail 213. This quick-release clamping device 212 effectively reduces the replacement time of the culture tank 5 and improves the overall work efficiency.

[0041] See also Figures 1 to 5 As shown, the cross lifting device 22 includes two bidirectional adjustment screw slides 221 distributed inside the first limit slide rail 211, and the two bidirectional adjustment screw slides 221 are synchronously driven by the gear transmission assembly 222. Two pushing links 223 are rotatably installed on the movable end of each bidirectional adjustment screw slide 221. The two pushing links 223 are cross-arranged and rotatably connected to each other. The pushing link 223 is equipped with a sliding push block 224 at one end away from the bidirectional adjustment screw slide 221. The sliding push block 224 is slidably connected to the lifting push plate 23, and the side of the sliding push block 224 is provided with anti-slip teeth. The cross lifting device 22 also includes a cross positioning device 225 installed between the mounting plate 21 and the lifting push plate 23.

[0042] The cross-lift mechanism 22 comprises two bidirectionally adjustable screw slides 221 located within the first limiting guide rail 211, which are synchronized via a gear transmission assembly 222. The gear transmission assembly 222, as the core of power transmission and synchronization control, ensures that the two bidirectionally adjustable screw slides 221 maintain the same motion state and speed during operation. When external power is input, the gear transmission assembly 222 evenly distributes the power to the two bidirectionally adjustable screw slides 221, causing them to start or stop synchronously, providing stable and coordinated power support for subsequent lifting and lowering adjustments.

[0043] Two push links 223 are rotatably mounted on the movable end of each bidirectional adjustment screw slide 221. The two push links 223 are cross-arranged and rotatably connected to each other. When the bidirectional adjustment screw slide 221 starts to move under the drive of the gear transmission assembly 222, the two push links 223 are driven to move crosswise. The end of the push link 223 away from the bidirectional adjustment screw slide 221 is equipped with a sliding push block 224, which is slidably connected to the lifting push plate 23. During the cross-movement of the push links 223, the sliding push block 224 moves synchronously on the lifting push plate 23. The cross-movement of the two push links 223 generates an upward thrust, which pushes the lifting push plate 23 up, causing the lifting push plate 23 to move away from the mounting plate 21, thereby providing more mounting positions for the support plate 3 to meet the needs of cultivating multiple culture tanks 5 at the same time.

[0044] When the lift plate 23 is raised to the desired position, the lift plate 23 and the anti-slip teeth on the sides of the sliding block 224 cooperate to achieve initial positioning. The anti-slip teeth increase the friction between the sliding block 224 and the lift plate 23, preventing the sliding block 224 from sliding freely on the lift plate 23, ensuring that the sliding block 224 can remain stably in the designated position, thereby ensuring the initial stability of the lift plate 23 after it is raised.

[0045] As the lift plate 23 and mounting plate 21 move away from each other, the cross-positioning device 225, installed between the mounting plate 21 and the lift plate 23, rises synchronously and changes state. When the lift plate 23 reaches the desired position, the cross-positioning device 225 is locked, performing a secondary positioning of the lift plate 23. The locking mechanism of the cross-positioning device 225 further enhances the stability of the lift plate 23 and provides additional support, preventing displacement or deformation of the lift plate 23 due to shaking during operation of the rocking table 1, thereby ensuring the structural stability of the entire adaptive support device.

[0046] The cross-connected design of the two push links 223 allows them to evenly distribute pressure from components such as the lift plate 23 and the culture tank 5 during the lifting and adjustment process. Furthermore, the overall support structure of the cross-connected lift mechanism 22 effectively resists external pressure, improving overall pressure resistance. This ensures that even with a heavy bacterial liquid in the culture tank 5, the mechanism can maintain stable operation and ensure smooth bacterial strain cultivation.

[0047] See also Figure 4 and Figure 5As shown, the cross positioning device 225 includes two cross-arranged hydraulic positioning rods, and the hydraulic positioning rod includes a rotating rod 2252 rotatably mounted on the mounting plate 21, and a suction chamber is provided inside the rotating rod 2252. A telescopic rod 2253 is slidably mounted on the rotating rod 2252, and a suction piston is provided at one end of the telescopic rod 2253. The suction piston is arranged inside the suction chamber, and the end of the telescopic rod 2253 away from the suction piston is rotatably connected to the lifting push plate 23. The cross positioning device 225 is also provided with an oil storage tank 2254, and the oil storage tank 2254 is provided with hydraulic oil. The output end of the oil storage tank 2254 is connected to the suction chamber, and the output end of the oil storage tank 2254 is installed with a control valve 2251.

[0048] During the adjustment process of the lifting and pushing plate 23 and the mounting plate 21 moving away from each other, the lifting and pushing plate 23 drives the telescopic rod 2253 to move, and the movement of the telescopic rod 2253 causes the rotating rod 2252 to rotate synchronously. At the same time, the telescopic rod 2253 drives the suction piston to move within the suction chamber. The movement of the suction piston generates a suction force within the suction chamber, causing the hydraulic oil in the oil storage tank 2254 to be sucked into the suction chamber through the output end of the oil storage tank 2254.

[0049] After the lift plate 23 is adjusted to the designated position, the operator operates the control valve 2251 to close the liquid outlet of the oil tank 2254. At this point, the hydraulic oil return channel between the suction chamber and the oil tank 2254 is blocked, preventing the hydraulic oil in the suction chamber from flowing back into the oil tank 2254. Because hydraulic oil is incompressible, its inability to flow back allows the telescopic rod 2253 to remain in place. Furthermore, the rotational connection between the telescopic rod 2253 and the lift plate 23 provides stable support for the lift plate 23, completing secondary positioning of the lift plate 23 and enhancing its stability. The two cross-arranged hydraulic positioning rods effectively prevent displacement or deformation of the lift plate 23 due to shaking during operation of the rocking table 1, thereby ensuring the structural stability of the entire adaptive support device.

[0050] See also Figures 1 to 8 As shown, two second limiting slide rails 231 are provided at the bottom of the lifting push plate 23, and the second limiting slide rails 231 are used to connect the sliding push block 224. The side of the second limiting slide rail 231 is provided with a limiting clamping mechanism, and the limiting clamping mechanism includes a limiting clamping strip 2321 installed on the side of the second limiting slide rail 231, and the side of the limiting clamping strip 2321 is provided with a plurality of limiting teeth, and the limiting teeth and the anti-slip teeth are clamped with each other. A second spring 2322 is installed between the limiting clamping strip 2321 and the second limiting slide rail 231, and an extrusion push block 233 is installed between the second limiting slide rail 231 and the limiting clamping strip 2321. The extrusion push block 233 is slidably connected to the limiting clamping strip 2321, and a separation protrusion is provided on the extrusion push block 233.

[0051] Two second limiting slide rails 231 are provided at the bottom of the lifting push plate 23, which cooperate with the sliding push block 224 in the cross lifting device 22 to provide a stable moving track for the sliding push block 224, ensuring that the sliding push block 224 can move according to the predetermined path during the lifting and adjustment process.

[0052] When the cross-lifting mechanism 22 adjusts the height of the lifting push plate 23, a worker applies a thrust to the extrusion push block 233 to move it. When the separation protrusion of the extrusion push block 233 moves between the second limiting rail 231 and the limiting clamping bar 2321, the separation protrusion pushes the limiting clamping bar 2321 away from the second limiting rail 231, separating the two. During this process, the second spring 2322 is squeezed and deformed. As the limiting clamping bar 2321 separates from the second limiting rail 231, the limiting teeth on the limiting clamping bar 2321 disengage from the anti-slip teeth on the side of the sliding push block 224. The sliding push block 224 can now slide freely within the second limiting rail 231, achieving position adjustment, thereby driving the lifting push plate 23 up or down to accommodate the cultivation needs of culture tanks 5 of different specifications, providing more mounting locations for the support plate 3, or accommodating the placement of larger culture tanks 5.

[0053] After the sliding push block 224 is adjusted to the designated position, the staff member adjusts the position of the extrusion push block 233 so that the separation protrusion of the extrusion push block 233 disengages the second limiting slide rail 231. At this time, the second spring 2322, under the action of its own elastic restoring force, pushes the limiting clamping strip 2321 back to its original position, so that the limiting teeth and anti-slip teeth re-engage each other. Securing the sliding push block 224 in the designated position prevents slipping during the operation of the lifting push plate 23, ensuring the smooth adjustment of the cross-lifting device 22, and thus ensuring the stability of the lifting push plate 23 during the operation of the rocking table 1, preventing displacement or deformation of the lifting push plate 23 due to shaking, ensuring the structural stability of the entire adaptive support device, and providing a stable environment for the cultivation of the strain used for rennet production.

[0054] See also Figure 1 and Figure 9 As shown, a limit mounting groove 31 is provided at the bottom of the support plate 3, and two limit card holes are provided on the inner side of the limit mounting groove 31. The limit card holes are connected to the sliding card strip 214. A plurality of limit clamping devices 33 are installed on the support plate 3. The limit clamping devices 33 are used to clamp the culture tank 5. An adsorption layer 34 is also installed on the support plate 3. The adsorption layer 34 is arranged above the limit clamping device 33, and a plurality of limit placement holes 35 are provided on the adsorption layer 34.

[0055] During bacterial culture operations, workers sequentially place multiple culture tanks 5 into the limiting placement holes 35 of the adsorption layer 34. The limiting placement holes 35 are designed according to the specifications of the culture tanks 5 and can serve as a preliminary limit for the culture tanks 5, ensuring that the culture tanks 5 are placed stably and preventing them from tipping over or shifting during subsequent operations.

[0056] After the culture tank 5 is placed, the staff aligns the limit mounting groove 31 of the support plate 3 with the guide mounting rail 213 on the mounting plate 21 and slides the support plate 3 along the guide mounting rail 213. During the sliding process, the support plate 3 squeezes the sliding clip 214 in the quick-release clamping device 212, causing the sliding clip 214 to retract into the guide mounting rail 213 and compressing the first spring 215. When the support plate 3 slides to the designated position and the limit clamping holes on both sides of the limit mounting groove 31 are aligned with the sliding clip 214, the first spring 215 recovers its deformation, pushing the sliding clip 214 outward and engaging with the limit clamping holes, thereby achieving the positioning and installation of the support plate 3 on the mounting plate 21.

[0057] After the support plate 3 is positioned and installed, the multiple limiting clamping devices 33 are triggered. The limiting clamping devices 33 simultaneously fix and clamp the multiple culture tanks 5, enhancing the stability of the culture tanks 5 during the shaking process, preventing the culture tanks 5 from shaking, colliding, or spilling the bacterial solution due to the shaking of the shaking table 1, and ensuring the smooth progress of strain cultivation.

[0058] Adsorption layer 34 has an adsorption function. During the incubation process, if bacterial liquid spills from the culture tank 5, adsorption layer 34 can promptly absorb the spilled liquid, preventing it from spreading to other areas and maintaining a clean incubation environment. Furthermore, adsorption layer 34 is detachably connected to support plate 3, making it easy to clean and replace adsorption layer 34 after incubation, ensuring its adsorption performance.

[0059] When the culture liquid culture is completed and the culture tank 5 needs to be replaced, the staff presses the push-unlocking block 216 on the mounting plate 21. The push-unlocking block 216 pushes the guide socket 2141 of the sliding card strip 214 through the V-shaped unlocking groove 217, causing the sliding card strip 214 to shrink and disengage from the limit card hole. At this time, the support plate 3 can be removed from the guide mounting rail 213, and the replacement operation of the culture tank 5 can be quickly completed, effectively reducing the replacement time, improving the overall work efficiency, and ensuring the continuity and efficiency of strain cultivation.

[0060] See also Figures 9 to 11As shown, the limiting clamping device 33 includes a plurality of fixed clamping blocks 331 fixedly mounted on the supporting plate 3, and a movable clamping block 332 is installed on the side of each fixed clamping block 331. The fixed clamping block 331 and the movable clamping block 332 are provided with a plurality of V-shaped clamping grooves 3321. The limiting clamping device 33 also includes a pushing rod 333 slidably mounted on the supporting plate 3, and a connecting push block 334 is slidably mounted on the pushing rod 333. A third spring 335 is installed between the connecting push block 334 and the pushing rod 333. Synchronous push rods 336 are rotatably installed on both sides of the connecting push block 334, and the synchronous push rod 336 is rotatably connected to the movable clamping block 332 at one end away from the connecting push block 334.

[0061] When performing bacterial liquid cultivation operations, the staff places multiple culture tanks 5 in the limiting placement holes 35 of the adsorption layer 34 in sequence. At this time, the culture tanks 5 stay in the V-shaped clamping grooves 3321 corresponding to the fixed clamping block 331 and the movable clamping block 332, and the limiting placement holes 35 play a preliminary limiting role for the culture tanks 5.

[0062] When the support plate 3 is mounted on the mounting plate 21, the mounting plate 21 exerts a compressive force on the push rod 333 of the position-limiting clamping device 33. This pressure overcomes the elastic force of the third spring 335 and moves toward the interior of the support plate 3, compressing the third spring 335. This, in turn, pushes the connecting push block 334. The movement of the connecting push block 334 also drives the synchronous push rods 336, which are rotatably mounted on either side, to move synchronously. The end of the synchronous push rod 336, which is distal to the connecting push block 334, is pivotally connected to the movable clamping block 332. Driven by the synchronous push rod 336, the movable clamping block 332 moves toward the fixed clamping block 331. As the movable clamping block 332 moves, the V-shaped clamping groove 3321 on the movable clamping block 332 applies pressure to the culture tank 5 , so that the culture tank 5 is tightly clamped and fixed between the fixed clamping block 331 and the V-shaped clamping groove 3321 of the movable clamping block 332 .

[0063] The V-shaped clamping groove 3321 can accommodate culture tanks 5 of varying diameters. As the diameter of the culture tank 5 changes, the inclined surface of the V-shaped clamping groove 3321 conforms well to the outer wall of the culture tank 5, ensuring stable clamping. Furthermore, the elastic pressure layer on the inner wall of the V-shaped clamping groove 3321 deforms elastically when subjected to pressure, further increasing friction with the outer wall of the culture tank 5 and preventing the culture tank 5 from slipping or shaking during shaking. This effectively enhances the stability of the culture tank 5 during shaking and ensures smooth bacterial strain cultivation.

[0064] When the culture tank 5 needs to be removed, the supporting plate 3 is removed from the mounting plate 21, the pushing rod 333 loses the pressure of the mounting plate 21, the third spring 335 recovers, and the connecting push block 334 loses the pushing force. At the same time, the V-shaped clamping groove 3321 of the movable clamping block 332 also loses the clamping force, so that the culture tank 5 can be easily removed.

[0065] See also Figures 3 to 12 As shown, the separation and buffering mechanism 4 includes a fixed frame 41 installed on the side of the mounting plate 21, a pressing frame 42 is sleeved on the outer side of the fixed frame 41, a plurality of fourth springs 43 are installed between the fixed frame 41 and the pressing frame 42, and a flexible partition 44 is installed on the fixed frame 41 and the pressing frame 42.

[0066] During the cultivation process of strains for rennet preparation, when the folding adjustment frame 2 is adjusted according to the cultivation requirements, and the cross-lifting device 22 is operated to drive the lifting push plate 23 to rise or retract to adjust the distance between the lifting push plate 23 and the mounting plate 21, the folding adjustment frame 2 will drive the separation buffer mechanism 4 installed on the side to move synchronously.

[0067] The flexible barrier layer 44 of the separating buffer mechanism 4 exhibits excellent flexibility and sealing properties. During movement, the flexible barrier layer 44 adheres tightly to the inner wall of the shaker 1, effectively sealing and separating the interior of the shaker 1, thereby dividing the interior of the shaker 1 into multiple independent cultivation spaces. This division of independent spaces enables the temperature control system of the shaker 1 to independently set and control the temperature of each zone, thereby simulating different environmental conditions for bacterial culture, greatly improving the device's adaptability to different bacterial strain cultivation environments.

[0068] When the rocking bed 1 is activated and causes the folding and adjusting frame 2 to rock, the rocking of the folding and adjusting frame 2 is transmitted to the fixed frame 41, causing the fixed frame 41 to move accordingly. During this movement, the fixed frame 41 exerts a compressive effect on the fourth spring 43. The fourth spring 43 has excellent elastic deformation capability and elastically deforms when squeezed, converting the kinetic energy transmitted from the fixed frame 41 into elastic potential energy for storage.

[0069] As the foldable adjustment frame 2 continues to shake, the fourth spring 43, under the action of the elastic restoring force, gradually releases the stored elastic potential energy, pushing the fixed frame 41 in the opposite direction, thereby buffering and restraining the shaking of the fixed frame 41. This buffering effect effectively prevents excessive shaking of the foldable adjustment frame 2 and reduces damage to the structure of the foldable adjustment frame 2 caused by the impact force of the shaking. At the same time, it ensures the stability of the foldable adjustment frame 2 during shaking, thereby ensuring the stability of the culture tank 5 mounted on the foldable adjustment frame 2 during shaking, providing a stable and reliable environment for strain cultivation.

[0070] Specific working principle: During the rennet production process, the culture liquid to be cultivated is first injected into the culture tank 5, and then multiple culture tanks 5 are sequentially placed on multiple support plates 3. The support plates 3 serve as a position limit for the culture tanks 5, ensuring the initial position stability of the culture tanks 5 during the cultivation process.

[0071] When the adaptive support device cooperates with the shaking table 1 to work normally, the lifting push plate 23 and the mounting plate 21 are in a folded state with each other. In the folded state, the stability during daily shaking can be ensured and the frequency of failure can be reduced. At this time, only the top mounting plate 21 can be installed with the support plate 3 to fix the culture tank 5. When there are many culture tanks 5 to be cultivated, in order to improve the cultivation efficiency, the staff operates the cross-lifting device 22 to drive the lifting push plate 23 to rise. During the rising process of the lifting push plate 23, it moves away from the mounting plate 21, so that the lifting push plate 23 and the mounting plate 21 are spread out with each other, thereby providing more installation positions for multiple support plates 3. By increasing the number of support plates 3 to fix more culture tanks 5, the cultivation efficiency can be effectively improved.

[0072] When encountering larger culture tanks 5, the operator adjusts the distance between the lifting and pushing plates 23 and the mounting plates 21 by retracting them, ensuring that the topmost mounting plate 21 has sufficient space to accommodate the cultivation requirements of larger culture tanks 5, thereby improving the device's adaptability to the culture tanks 5. As the mounting plates 21 are moved and adjusted, they simultaneously drive the partitioning and buffering mechanisms 4 to move upward and downward. The partitioning and buffering mechanisms 4 effectively separate the cultivation space within the shaker 1, forming multiple independent cultivation spaces. By coordinating with the shaker 1's temperature control system, the temperature can be independently controlled for each zone, enabling simultaneous simulation of different environmental conditions for cultivating bacterial fluids, further improving cultivation adaptability. When the bacterial fluid within the culture tanks 5 needs to be cultivated, multiple support plates 3 are directly inserted onto the mounting plates 21, which then position and secure the support plates 3. Once the support plates 3 are in place, they further secure the culture tanks 5, ensuring their installation stability during operation. After the shaker 1 is adjusted to an appropriate internal temperature, ensuring that each cultivation space maintains a suitable environment, the adaptive support mechanism is then driven to shake. During the shaking process, the bacterial liquid in the culture tank 5 shakes synchronously, promoting full contact and uniform distribution of the bacterial liquid and nutrients. The separation buffer mechanism 4 is arranged between the shaker 1 and the mounting plate 21, which effectively reduces the rocking force of the lifting push plate 23 and the mounting plate 21. At the same time, the guide column 24 and the cross lifting device 22 provide support to jointly ensure the strength and stability of the adaptive support device during the overall shaking, and avoid damage to the adaptive support device structure or displacement of the culture tank 5 due to shaking. When the bacterial liquid is cultured, the staff can directly remove the support plate 3 from the mounting plate 21, and then quickly install the support plate 3 with the new culture tank 5. This design effectively reduces the replacement time of the culture tank 5, improves the overall work efficiency, and ensures the continuity and efficiency of strain cultivation.

[0073] The above embodiments merely represent one or more embodiments of the present invention, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of protection of the present invention. It should be noted that a person of ordinary skill in the art may make various modifications and improvements without departing from the spirit of the present invention, and such modifications and improvements fall within the scope of protection of the present invention. Therefore, the scope of protection of the present invention shall be subject to the appended claims.

Claims

1. A bacterial strain cultivation device for preparing rennet, comprising a shaking table (1) and an adaptive supporting device mounted on the movable end of the shaking table (1), characterized in that: The adaptive supporting device includes a folding adjustment frame (2) fixedly mounted on the movable end of the shaking table (1), the folding adjustment frame (2) includes a plurality of mounting plates (21), a lifting push plate (23) is provided between adjacent mounting plates (21), the lifting push plate (23) is fixedly connected to the bottom of the mounting plate (21), the lifting push plate (23) and the mounting plate (21) are connected in series via a guide column (24), the lifting push plate (23) and the mounting plate (21) are both slidably connected to the guide column (24), a cross lifting device (22) is installed between the lifting push plate (23) and the mounting plate (21), the cross lifting device (22) is used to lift and adjust the distance between the lifting push plate (23) and the mounting plate (21), a detachable support plate (3) is installed on the mounting plate (21), the support plate (3) is used to support the culture tank (5), and a separation buffer mechanism (4) is installed on the outer side of each mounting plate (21), and the outer side of the separation buffer mechanism (4) contacts the inner wall of the shaking table (1); Two first limiting slide rails (211) are installed on the mounting plate (21), and a quick-release clamping device (212) is also installed on the mounting plate (21). The quick-release clamping device (212) includes a guide mounting rail (213), and sliding clamping strips (214) are slidably installed on both sides of the guide mounting rail (213). The side of the sliding clamping strip (214) is provided with a guide socket (2141), and a first spring (215) is installed between each sliding clamping strip (214) and the guide mounting rail (213). A push-unlocking block (216) is also slidably installed on the guide mounting rail (213), and one end of the push-unlocking block (216) is provided with a V-shaped unlocking groove (217), and the V-shaped unlocking groove (217) is slidably docked with the guide socket (2141).

2. The bacterial strain cultivation device for preparing rennet according to claim 1, characterized in that: The cross lifting device (22) includes two bidirectional adjustment screw slides (221) distributed inside the first limit slide rail (211), the two bidirectional adjustment screw slides (221) are synchronously driven by the gear transmission assembly (222), and two push links (223) are rotatably installed on the movable end of each bidirectional adjustment screw slide (221), the two push links (223) are cross-arranged and rotatably connected to each other, and a sliding push block (224) is installed at one end of the push link (223) away from the bidirectional adjustment screw slide (221), and the sliding push block (224) is slidably connected to the lifting push plate (23), and the side of the sliding push block (224) is provided with anti-slip teeth. The cross lifting device (22) also includes a cross positioning device (225) installed between the mounting plate (21) and the lifting push plate (23).

3. The bacterial strain cultivation device for preparing rennet according to claim 2, characterized in that: The cross positioning device (225) includes two cross-arranged hydraulic positioning rods, each of which includes a rotating rod (2252) rotatably mounted on a mounting plate (21), a suction chamber being provided inside the rotating rod (2252), a telescopic rod (2253) being slidably mounted on the rotating rod (2252), a suction piston being provided at one end of the telescopic rod (2253), the suction piston being provided inside the suction chamber, and an end of the telescopic rod (2253) away from the suction piston being rotatably connected to the lifting push plate (23). The cross positioning device (225) is further provided with an oil storage tank (2254), hydraulic oil being provided inside the oil storage tank (2254), an output end of the oil storage tank (2254) being connected to the suction chamber, and a control valve (2251) being mounted at the output end of the oil storage tank (2254).

4. The bacterial strain cultivation device for preparing chymosin according to claim 2, characterized in that: Two second limiting slide rails (231) are provided at the bottom of the lifting push plate (23), and the second limiting slide rails (231) are used to connect the sliding push block (224). A limiting clamping mechanism is provided on the side of the second limiting slide rail (231), and the limiting clamping mechanism includes a limiting clamping strip (2321) installed on the side of the second limiting slide rail (231). The side of the limiting clamping strip (2321) is provided with a plurality of limiting teeth, and the limiting teeth and the anti-slip teeth are clamped with each other. A second spring (2322) is installed between the limiting clamping strip (2321) and the second limiting slide rail (231), and an extrusion push block (233) is installed between the second limiting slide rail (231) and the limiting clamping strip (2321). The extrusion push block (233) is slidably connected to the limiting clamping strip (2321), and a separation protrusion is provided on the extrusion push block (233).

5. The bacterial strain cultivation device for preparing rennet according to claim 1, characterized in that: A limit installation groove (31) is provided at the bottom of the support plate (3), two limit clamping holes are provided on the inner side of the limit installation groove (31), and the limit clamping holes are connected to the sliding clamping strip (214). A plurality of limit clamping devices (33) are installed on the support plate (3), and the limit clamping devices (33) are used to clamp the culture tank (5). An adsorption layer (34) is also installed on the support plate (3), and the adsorption layer (34) is arranged above the limit clamping device (33). The adsorption layer (34) is provided with a plurality of limit placement holes (35).

6. The bacterial strain cultivation device for preparing rennet according to claim 5, characterized in that: The position-limiting clamping device (33) includes a plurality of fixed clamping blocks (331) fixedly mounted on the supporting plate (3), a movable clamping block (332) being mounted on the side of each fixed clamping block (331), a plurality of V-shaped clamping grooves (3321) being provided on the fixed clamping block (331) and the movable clamping block (332), the position-limiting clamping device (33) further includes a pushing rod (333) slidably mounted on the supporting plate (3), a connecting push block (334) being slidably mounted on the pushing rod (333), a third spring (335) being mounted between the connecting push block (334) and the pushing rod (333), synchronous push rods (336) being rotatably mounted on both sides of the connecting push block (334), and an end of the synchronous push rod (336) away from the connecting push block (334) being rotatably connected to the movable clamping block (332).

7. The bacterial strain cultivation device for preparing rennet according to claim 1, characterized in that: The separation buffer mechanism (4) includes a fixed frame (41) mounted on the side of the mounting plate (21), a pressing frame (42) is sleeved on the outer side of the fixed frame (41), a plurality of fourth springs (43) are mounted between the fixed frame (41) and the pressing frame (42), and a flexible barrier layer (44) is mounted on the fixed frame (41) and the pressing frame (42).

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