Enzymatic reaction complete device and process for separating lignocellulose components through enzymolysis

Abstract

The invention relates to the technical field of lignocellulose enzymolysis reaction, and mainly discloses an enzyme reaction complete device and process for enzymolysis separation of lignocellulose components, and the complete device is composed of a first-stage enzyme catalysis reactor, a second-stage enzyme catalysis reactor, a third-stage light enzyme catalysis reactor and a fourth-stage enzyme catalysis reactor which are connected in sequence. A photo-enzyme reaction is taken as a core, an equipment structure for separating components through gradual layering enzymolysis of a compound enzyme is reasonably designed, components for separating lignocellulose through gradual layering enzymolysis of the compound enzyme are reasonably designed, and different stirring structures arranged in each stage of enzyme catalytic reactor exert respective advantages, so that materials are fully mixed with the enzyme, and the catalytic reaction of the enzyme is accelerated; the reaction is uniform and thorough, and the maximum reaction efficiency is realized.

Description

Technical Field

[0001] The invention relates to the technical field of lignocellulose enzymatic hydrolysis reaction, in particular to an enzyme-photoenzyme reaction complete device and process for biological enzymatic hydrolysis and separation of lignocellulose components. Background Art

[0002] Lignocellulosic straw, including wheat straw, rice straw, corn straw, cotton straw, sugarcane bagasse, and reeds, is an important biomass resource and a major source of lignocellulose. They are similar in composition and structure, primarily consisting of cellulose, hemicellulose, and lignin.

[0003] Cellulose is mainly composed of repeating cellobiose units and is a β Hemicellulose is a homogeneous, long-chain polymer composed of 2-D-glucose linked by glycosidic bonds, with a structure consisting of crystalline and amorphous regions. Hemicellulose is primarily composed of several different types of monosaccharides, including pentoses (such as xylose and arabinose), hexoses (such as galactose, mannose, and glucose), and uronic acids (such as galacturonic acid), making it a heterogeneous polysaccharide. Grass straw hemicellulose is primarily composed of polyarabinan-4-O-methylglucuronic acid xylose. Lignin is a high-molecular-weight aromatic compound formed by a three-dimensional network of phenylpropane units linked by carbon-carbon and ether bonds. The three monomers that make up lignin are guaiacylpropane, syringylpropane, and p-hydroxyphenylpropane, which are covalently linked to form a complex three-dimensional network structure. Lignin is tightly linked to hemicellulose through ferulic acid and p-coumaric acid. Some lignin is chemically bonded to cellulose, forming the straw cell wall structure, which protects the plant. However, this complex structure also limits the efficient utilization of lignocellulose.

[0004] In the traditional industrial production of separating lignocellulosic fiber components, the most effective way to industrially utilize lignocellulosic straw is the straw pulping process using high-temperature strong alkali cooking and the xylose (furfural) production process using high-temperature strong acid cooking. The problem with the existing process is that it produces black liquor or wastewater containing lignin components that is difficult to treat, making it an industrial project that is restricted or prohibited from development.

[0005] Enzymatic separation of lignocellulose components has become a highly beneficial process. Enzymatic catalysis offers the advantages of being green, sustainable, and controllable. It not only produces no environmental pollution but also produces a wide variety of products with high added value. It is considered a potential solution to the environmental, efficiency, and stereochemical challenges faced by traditional processes. The rapid development of synthetic biology has driven the industrialization of industrial enzyme preparations. While enzyme design and modification can enhance enzyme catalytic activity, the synergistic effects of enzymes and non-enzymatic molecules also play a crucial role, such as in photoenzymatic reactions. Light is an environmentally friendly, abundant, and clean energy source, making it considered an ideal green reagent. Photoenzymatic catalysis generates reactive chemical intermediates under mild conditions. Photoenzymatic catalysis not only utilizes light to regenerate cofactors to unleash the enzyme's natural activity but also can trigger unnatural reactions, thereby acquiring novel catalytic functions. Furthermore, by leveraging the high selectivity and amenable to directed evolution, photoenzymatic catalysis can manipulate reactive intermediates triggered by light, offering new solutions to the challenges of stereochemical control in photochemistry. Photoenzyme catalysis has been successfully used to catalyze important biochemical reactions, including carbon-carbon, carbon-nitrogen, carbon-oxygen, and carbon-halogen bonds, demonstrating unique advantages in catalytic activity, reaction selectivity, and substrate spectrum expansion. The development of new, highly efficient photocatalysts has enriched the mechanistic pathways of photocatalysis, playing a particularly important role in the photocatalytic reactions of lignocellulase.

[0006] Due to the complex structure of lignocellulose, which comprises multiple components, including cellulose, hemicellulose, and lignin, with significantly different chemical structures and physical properties, a single complex enzyme combination is difficult to efficiently separate these three components. Existing equipment and processes typically use a single reaction system, which cannot meet the complex requirements of the enzyme-photoenzyme catalysis required to separate lignocellulose components.

[0007] Chinese invention patent CN116479670B discloses a process for multi-step enzymatic hydrolysis and separation of plant straw fiber components using a combined enzyme and photoenzyme. Combination enzymes are formed according to the order of straw enzymatic hydrolysis sites and their enzymatic properties, providing four sets of composite enzymes. These enzymes are then combined with auxiliary promoters in the enzymatic hydrolysis reaction and added sequentially to different enzymatic hydrolysis reactors for step-by-step enzymatic hydrolysis and separation of plant straw fiber components.

[0008] Existing processes and equipment are limited in several key areas: During the enzymatic hydrolysis process to separate the lignocellulose components, the physical properties of the straw material change after the primary enzyme-catalyzed reaction, one of which is softening. Because the density of the straw material is lower than that of the fermentation liquid or enzymatic hydrolysis solution, the straw tends to float. After entering the vertical second and third stage enzymatic hydrolysis reactors, the floating straw reduces the contact area with the enzyme preparation, thus affecting the enzymatic hydrolysis efficiency.

[0009] 2. Based on the enzyme activity requirements of the third-stage enzymatic hydrolysis reaction, the cooling device usually adopts a coil or jacket for cooling. However, since the tank of the third-stage enzymatic hydrolysis reactor needs to have a light-passing port necessary for the photoenzymatic reaction, the coil cooling method is not feasible. If a jacket structure is used, it needs to be provided with a through hole in the jacket as well, and its manufacturing and assembly process are relatively cumbersome, which greatly increases the manufacturing cost of the reactor tank.

[0010] 3. In the fourth stage enzymatic hydrolysis reaction, after the first and second stage enzymatic reactions and the third stage photoenzymatic reaction, the material is in a slurry state and is placed in the fourth stage enzymatic hydrolysis reactor for static reaction. The fourth stage enzymatic hydrolysis reaction takes a long time. Large volumes of slurry materials are prone to stratification or localized excessive concentration when left to stand, which is not conducive to sufficient contact between the enzyme and the substrate, thereby affecting the efficiency of the fourth stage enzymatic hydrolysis reaction. Summary of the Invention

[0011] The purpose of the present invention is to solve the technical problems existing in the prior art. Based on the requirements of the above-mentioned process technology for equipment, the present invention provides an enzymatic reaction complete set of equipment and process for enzymatic hydrolysis and separation of lignocellulose components. According to the characteristics of the enzyme-catalyzed reaction and the state changes of the material in the reaction system, the efficiency of the enzymatic hydrolysis reaction at each level is further improved, thereby reducing the enzymatic hydrolysis reaction time, realizing the enzyme-photoenzyme catalytic reaction for separating the lignocellulose components, and dividing the lignocellulose into cellulose, hemicellulose pentose and lignin components.

[0012] To achieve the above object, the present invention adopts the following technical solution: an enzymatic reaction set for enzymatic hydrolysis and separation of lignocellulose components, comprising a first-stage enzymatic reactor, a second-stage enzymatic reactor, a third-stage photoenzymatic reactor and a fourth-stage enzymatic reactor connected in sequence; The complete set of devices of the present invention realizes a reaction catalysis system with enzyme-photoenzyme catalytic reaction as the core. The first and second stage enzyme catalytic reactions of the photoenzyme catalytic reaction can be regarded as the "pretreatment" of the third stage photoenzyme catalytic reaction. The fourth stage enzyme catalytic reaction after the third stage photoenzyme catalytic reaction is the "deep treatment" stage of the enzyme-photoenzyme catalytic reaction to adjust the thoroughness of the catalytic reaction.

[0013] The first-stage enzyme catalytic reactor is a horizontal reactor with a horizontal stirring structure, the second-stage enzyme catalytic reactor is a horizontal reactor with a vertical stirring structure, and the two vertical stirring structures are arranged at both ends of the second-stage enzyme catalytic reactor, the third-stage photoenzyme catalytic reactor is a horizontal reactor with a vertical stirring structure, and the two vertical stirring structures are arranged at both ends of the second-stage enzyme catalytic reactor, and the fourth-stage enzyme catalytic reactor is a vertical reactor with a vertical stirring structure; the "complete set" of the present invention is composed of a plurality of multi-stage enzyme catalytic reactors with stirring forms. According to the different material properties, this "complete set" has structures such as horizontal stirring and vertical stirring spiral propulsion. The physical form of the clean straw flakes material just entering the first-stage enzyme catalytic reactor does not change much, and the material floats on the upper layer. Horizontal stirring is used, and the mechanical power is small, which makes it easy to stir the material into the liquid phase reaction catalytic system; after the previous catalytic reaction, the material form changes from hard to soft, and enters the second-stage enzyme catalytic reactor or the third-stage photoenzyme catalytic reactor. Vertical stirring is used to achieve rapid stirring, thorough catalytic reaction, and reduce raw materials; the fourth-stage enzymatic hydrolysis reaction takes a long time, and large-volume slurry materials are prone to stratification or local excessive concentration when standing. The use of a vertical stirring structure in the larger-volume fourth-stage enzyme catalytic reactor is conducive to sufficient contact between the enzyme and the substrate, thereby improving the efficiency of the fourth-stage enzymatic hydrolysis reaction.

[0014] The first-stage enzyme catalytic reactor, the second-stage enzyme catalytic reactor, the third-stage photoenzyme catalytic reactor and the fourth-stage enzyme catalytic reactor are all provided with a feed port and a discharge port, and the discharge port of the upper-stage reactor is connected to the feed port of the lower-stage reactor; the first-stage enzyme catalytic reactor, the second-stage enzyme catalytic reactor and the third-stage photoenzyme catalytic reactor are all provided with an enzyme preparation addition port, an enzyme auxiliary agent addition port, a pH regulator addition port, a breathing valve interface and a spare port, and are also provided with a temperature sensor, a pressure sensor, a pH sensor and a liquid level sensor; The first-stage enzyme catalytic reactor is provided with a steam heating device and an auxiliary heating device; the second-stage enzyme catalytic reactor is provided with a cooling device; the third-stage photoenzyme catalytic reactor is evenly distributed with multiple light holes; the multiple light holes are sealed with glass sheets with good light transmittance, and an external light source of a certain intensity illuminates the photoenzyme catalytic reaction system through the light holes.

[0015] The volume of the second-stage enzyme catalytic reactor and the third-stage photoenzyme catalytic reactor is 70%-80% of the volume of the first-stage enzyme catalytic reactor, and the volume of the fourth-stage enzyme catalytic reactor is 3-5 times the volume of the first-stage enzyme catalytic reactor. In the second-stage enzyme catalytic reactor and the third-stage photoenzyme catalytic reactor, the raw material softens and enters the liquid phase catalytic volume, resulting in a smaller "volume" than in the first-stage enzyme catalytic reactor. Therefore, the volume of the second-stage and third-stage enzyme catalytic reactors is reduced, which reduces the manufacturing cost and facilitates uniform stirring. The fourth-stage enzyme catalytic reaction time is 3-5 times that of the first-stage enzyme catalytic reaction time. Therefore, the volume of the fourth-stage enzyme catalytic reactor is 3-5 times the volume of the first-stage enzyme catalytic reactor, thus enabling continuous production of the complete set of equipment.

[0016] The structure of lignocellulose straw is composed of three components: hydrophobic lignin, hydrophilic hemicellulose, and cellulose. Cellulose molecules are interwoven into bundles and dispersed in the hemicellulose and lignin components, forming a structure similar to "reinforced concrete." Based on these characteristics, a device structure is designed with photoenzyme reaction as the core, and a rational design of the compound enzyme gradually layered enzymatic hydrolysis and separation of components. The reactions in the first and second stage enzyme reactors are front-end enzyme catalytic reactions, which loosen the lignocellulose straw tissue and facilitate the photoenzyme catalytic reaction in the third stage photoenzyme reactor, allowing it to exert its maximum catalytic effect. The fourth stage enzyme reactor is an enzyme-catalyzed deep reaction, which supplements the deficiencies of the photoenzyme catalytic reaction. The equipment structure with the optimal temperature, pH value, concentration and other conditions for different enzyme activities is set in different reactors, so that different reactors can play their own roles and form an integral whole.

[0017] The horizontal stirring structure of the first-stage enzymatic hydrolysis reactor includes a first stirring shaft and a first stirring blade arranged on the first stirring shaft; the vertical stirring structure of the second-stage enzymatic hydrolysis reactor includes a second stirring shaft and a second stirring blade arranged on the second stirring shaft; the vertical stirring structure of the third-stage photoenzymatic hydrolysis reactor includes a third stirring shaft and a third stirring blade arranged on the third stirring shaft; the vertical stirring structure of the fourth-stage enzymatic hydrolysis reactor includes a fourth stirring shaft and a fourth stirring blade arranged on the fourth stirring shaft.

[0018] The steam heating device is a steam generator, which is connected to the first reactor tank through a steam interface. The auxiliary heating device is a heating coil, which is evenly distributed on the outer wall of the first reactor tank; the cooling device is a jacket, which is arranged on the outer wall of the second reactor tank.

[0019] The exteriors of the first-stage enzyme catalytic reactor, the second-stage enzyme catalytic reactor and the fourth-stage enzyme catalytic reactor are all provided with insulation layers.

[0020] Specifically, the first reactor tank body of the first-stage enzyme catalytic reactor is a horizontal tank body structure, the axis of the first stirring shaft is parallel to the axis of the first reactor tank body, and two groups of first stirring blades are symmetrically arranged along the center line of the axis of the first reactor tank body on the first stirring shaft. Preferably, the first stirring blade is a spiral ribbon stirring blade, and the two groups of first stirring blades circulate and stir, so that the raw materials are pushed toward the center from the two ends of the reactor tank body; the second reactor tank body of the second-stage enzyme catalytic reactor is a horizontal tank body structure, and two groups of second stirring shafts are symmetrically arranged along the center line of the axis of the second reactor tank body, the axis of the second stirring shaft is perpendicular to the axis of the second reactor tank body, and each group of second stirring shafts is provided with a second stirring blade, preferably Preferably, the second stirring blade is a paddle stirring blade; the third reactor tank body of the third-stage photoenzyme catalytic reactor is a horizontal tank body structure, and two groups of third stirring shafts are symmetrically arranged along the midline of the axis of the third reactor tank body, and the axes of the third stirring shafts are perpendicular to the axis of the third reactor tank body. Each group of third stirring shafts is provided with a third stirring blade, and preferably, the third stirring blade is a paddle stirring blade; the fourth reactor tank body of the fourth-stage enzyme catalytic reactor is a vertical tank body structure, and a fourth stirring shaft parallel to the axis of the fourth reactor tank body is provided in the middle of the fourth reactor tank body, and a fourth stirring blade is provided in the middle of the fourth stirring shaft, and preferably, the fourth stirring blade is a gate stirring blade.

[0021] The fourth-stage enzymatic hydrolysis reactor is also provided with a central axis screw propeller, and the bottom of the fourth-stage enzyme catalytic reactor is also provided with a bottom screw propeller. Specifically, the central axis screw propeller uses a fourth stirring shaft as a screw, and a single spiral ribbon is provided in the middle of the fourth stirring shaft. The bottom screw propeller drives the single spiral ribbon screw to rotate through a motor. Since the fourth discharge port is provided in the middle of the bottom of the fourth reactor tank, the single spiral ribbon on the screw of the bottom screw propeller is symmetrically arranged along the center line of the screw, and the spiral directions are opposite. After the enzyme reaction is completed, the material is pushed out by the spiral propeller at the bottom of the reactor and pumped into the next stage of the solid-liquid separation process.

[0022] In the first-stage enzyme catalytic reactor, the first stirring shaft is connected to the first stirring motor, the first feed port is arranged at the top of the first reactor tank body, and a valve is provided on the first feed port, the first discharge port is arranged at the bottom of the first reactor tank body, and a valve is also provided on the first discharge port, the steam interface is arranged at the bottom side of the first reactor tank body, and multiple steam interfaces are provided, and a muffler is provided on the steam interface, which has a porous structure, the first breathing valve interface is arranged at the top of the first reactor tank body, and a breathing regulating valve is provided on the first breathing valve interface; the first reactor tank body is also provided with a first pressure sensor access port, a first pH sensor interface and a first temperature sensor interface for installing a pressure sensor, a pH sensor and a temperature sensor respectively; it is also provided with a first manhole, a first pressure safety valve interface and two reserved ports, namely a first reserved port and a second reserved port.

[0023] In the second-stage enzyme catalytic reactor, the second stirring shaft is connected to the second stirring motor, the second feed port is arranged at the top of the second reactor tank body, and a valve is provided on the second feed port, the second discharge port is arranged at the bottom of the second reactor tank body, and a valve is also provided on the second discharge port, the second breathing valve interface is arranged at the top of the second reactor tank body, and a breathing regulating valve is provided on the second breathing valve interface; the second reactor tank body is also provided with a second pressure sensor access port, a second pH sensor interface and a second temperature sensor interface for installing a pressure sensor, a pH sensor and a temperature sensor respectively; a second manhole and a third reserved port are also provided.

[0024] In the third-stage photoenzyme catalytic reactor, the third stirring shaft is connected to the third stirring motor, the third feed port is arranged at the top of the third reactor tank body, and a valve is arranged on the third feed port, the third discharge port is arranged at the bottom of the third reactor tank body, and a valve is also arranged on the third discharge port, the third breathing valve interface is arranged at the top of the reactor tank body, and a breathing regulating valve is arranged on the third breathing valve interface; the third reactor tank body is also provided with a third pressure sensor access port, a third pH sensor interface and a third temperature sensor interface for installing a pressure sensor, a pH sensor and a temperature sensor respectively; a third manhole and a fourth reserved port are also provided.

[0025] In the fourth stage enzyme catalytic reactor, the fourth feed port is arranged at the top of the fourth reactor tank body, and a valve is arranged on the fourth feed port; the fourth discharge port is arranged at the bottom of the fourth reactor tank body, and a valve is also arranged on the fourth discharge port.

[0026] The enzyme-photoenzyme reaction set for enzymatic hydrolysis and separation of lignocellulose components of the present invention has the following working principle for the enzymatic catalytic separation of lignocellulose components in the complete set of equipment: Based on the structural characteristics of lignocellulose, the characteristics of the specificity of biological enzymes and the characteristics of application scenarios, the "one-pot" catalytic reaction of enzymes is a rational method, which is difficult to achieve in actual catalytic reaction systems. In the catalytic reactors of this complete set of equipment, different composite enzymes are added to the primary, secondary, tertiary and quaternary enzyme catalytic reactors respectively according to the flow path of the material in the reactor to complete catalytic reactions with different goals. The reactors at each level can adapt to the different sites of enzymatic hydrolysis of lignocellulose components, different application scenarios of composite enzymes (temperature, pH, etc.), and different physical forms of materials. The use of multiple single-unit reactors to form a complete set of equipment is an effective guarantee for completing the graded enzymatic hydrolysis and separation of plant components of complex lignocellulose components.

[0027] An enzymatic reaction process for separating lignocellulose components by enzymatic hydrolysis comprises the following steps: S1. The cleaned straw pieces are fed into the first-stage enzyme reactor, and water is added according to a solid-liquid ratio of 1:5-8 to form a reaction system; the stirring speed is 5-15 rpm, and the stirring mode is circulating stirring. The corresponding complex enzyme preparation, enzyme additive, and pH adjuster are added to the first-stage enzyme reactor; high-temperature steam is introduced into the first-stage enzyme reactor to heat the material to the optimal enzyme activity temperature of the first-stage enzyme catalytic reaction; the temperature, pressure, pH, liquid level and other sensors on the first-stage enzyme catalytic reaction are used to feedback and adjust the enzyme reaction to maintain the optimal conditions until the first-stage enzyme catalytic reaction is completed; S2. After the reaction in the first-stage enzyme-catalyzed reactor is completed, the material enters the second-stage enzyme-catalyzed reactor with a stirring speed of 50-150 rpm and a stirring mode of stirring in the same direction, intermittent forward and reverse directions; the corresponding complex enzyme preparation, enzyme aid, and pH regulator are added to the second-stage enzyme-catalyzed reactor; the jacket circulating water is cooled to the optimal enzyme activity temperature of 85-110°C for the second-stage enzyme-catalyzed reaction; the temperature, pressure, pH, liquid level and other sensors on the second-stage enzyme-catalyzed reactor are feedback-regulated to maintain the optimal enzyme reaction conditions until the second-stage enzyme-catalyzed reaction is completed; in S2, 10 minutes before the end of the second-stage enzyme-catalyzed reaction, the material is further cooled to the optimal enzyme activity reaction temperature of 65-75°C for the third-stage photoenzyme-catalyzed reaction; S3. After the reaction in the second-stage enzyme-catalyzed reactor is completed, the material enters the third-stage photoenzyme-catalyzed reactor with a stirring speed of 50-150 rpm and a stirring mode of stirring in the same direction, intermittent forward and reverse directions; the corresponding complex enzyme preparation, enzyme aid, and pH regulator are added to the third-stage photoenzyme-catalyzed reactor; the light port on the reactor tank is irradiated under certain light intensity conditions of an external light source; the temperature, pressure, pH, liquid level and other sensors on the reactor tank are used to adjust the feedback to maintain the optimal conditions for the enzyme reaction until the third-stage photoenzyme-catalyzed reaction is completed; S4. After the reaction in the third-stage photoenzyme catalytic reactor is completed, the material enters the fourth-stage enzyme catalytic reactor with a stirring speed of 20-60 rpm and a circulating stirring method. The temperature is maintained at 40-60°C using a natural trend cooling method. After the fourth-stage enzyme catalytic reaction is completed, the material is pumped into the next stage of solid-liquid separation equipment.

[0028] Preferably, in S1, the size of the clean straw flakes after processing and cleaning is 2-3 cm.

[0029] Preferably, in S2, when high-temperature steam is introduced into the first-stage enzyme catalytic reactor to heat the material, synergistic heating is performed through the heating coil.

[0030] Preferably, in S3, 5 minutes before the completion of the third-stage photoenzyme catalysis, the complex enzyme preparation required for the fourth-stage catalytic reaction is added.

[0031] Preferably, in S4, the material is delivered out through the middle axis screw propeller and the bottom screw propeller after the fourth stage enzyme catalysis reaction is completed.

[0032] Compared with the prior art, the present invention has the following beneficial effects: The present invention provides an enzyme and photoenzyme complete reaction device and process for enzymatically separating lignocellulose components. The complete device is a complete system consisting of multi-stage reactors. Based on the characteristics of the enzyme catalytic reaction and the state changes of the materials in the reaction system, the reactor structure and process show excellent effects: (1) The device of the present invention adopts a horizontal reactor with a vertical stirring structure in the structure of the second-stage enzyme reactor and the third-stage photoenzyme reactor. Compared with the prior art, the cross-sectional area of ​​the reaction vessel is increased and its height is reduced; the length of the vertical stirrer is reduced, and the number of vertical stirrers is increased.

[0033] 1. Considering the shortened agitator shaft: Due to its shorter shaft, a lower-power motor can achieve higher agitation speeds, making it easier for the raw materials to enter the liquid-phase catalytic reaction system. Compared with vertical reactors of the same volume, horizontal reactors require approximately 50% less power. This design is particularly suitable for processing straw, a raw material that tends to float, as high-speed agitation ensures uniform contact between the straw and the enzyme preparation. It also saves energy. The shorter agitator shaft reduces wear and the risk of failure during the agitation process.

[0034] 2. From the perspective of increasing the cross-sectional area and reducing the height: The design of increasing the cross-sectional area and reducing the height can make the reaction vessel more adaptable to the characteristics of softened straw. The softened straw is easier to distribute evenly within the container, and has more complete contact with the enzyme preparation. By reducing the height of the container, the static pressure of the liquid can be reduced, thereby reducing the risk of straw floating up. At the same time, the larger cross-sectional area also makes it easier for the agitator to redistribute the floating straw into the liquid during the stirring process. The uniform distribution means that more straw raw material can come into contact with the enzyme preparation and react, thereby improving the overall enzymatic hydrolysis efficiency.

[0035] (2) In the device of the present invention, a cooling structure is not provided on the third-stage enzymatic hydrolysis reactor. Instead, the second-stage enzyme reactor is used as a pre-cooling structure of the third-stage enzymatic hydrolysis reactor, thereby avoiding the problem that it is difficult to provide a cooling device on the third-stage enzymatic hydrolysis reactor due to the distribution of light ports. In this way, the manufacturing cost of the third-stage enzymatic hydrolysis reactor can be reduced while meeting the light intensity requirement of the third-stage enzymatic hydrolysis reaction, thereby significantly improving the efficiency of the photoenzymatic hydrolysis reaction.

[0036] (3) The fourth-stage enzyme reactor in the device of the present invention uses a gate-type stirring blade to generate a stirring effect in the horizontal direction, breaking up the agglomeration and stratification of the materials. It is particularly suitable for large-volume reaction systems, thereby significantly improving the reaction efficiency. The vertical central axis screw propeller in the fourth-stage enzymatic hydrolysis reactor of the present invention can generate an axial flow, effectively pushing the material from top to bottom in the reactor; it can form a three-dimensional stirring effect, ensuring that the material can be fully stirred and mixed in the middle and lower parts of the entire reactor. Through the coordinated stirring of the vertical screw propeller and the gate-type stirring blade, the fluid velocity distribution in the reactor will become more uniform, avoiding the reaction unevenness caused by local excessively fast or slow flow rates.

[0037] (4) According to the optimum temperature requirements of enzyme catalysis reaction, the present invention adopts a step-by-step cooling reaction from high temperature, with the first stage enzyme catalysis reaction having the highest temperature and the fourth stage enzyme catalysis reaction having the lowest temperature. The first stage enzyme catalysis reactor is directly heated quickly by steam and has an external coil auxiliary heating structure. The external coil auxiliary heating has two functions: in the pre-reaction stage, it assists the reactor heating function; in the reaction stage, it regulates the heat preservation function and maintains the most suitable temperature for enzyme catalysis reaction. The second stage enzyme catalysis reactor is provided with a jacket structure, in which cold water is used for cooling, and has two functions: first, it reaches the optimum enzyme activity reaction temperature of the second stage enzyme catalysis; before the end of the second stage enzyme catalysis reaction, it continues to cool the liquid to the optimum enzyme activity reaction temperature of the third stage light enzyme catalysis reaction; and the fourth stage enzyme catalysis reaction adopts a natural trend cooling method due to the low temperature and wide optimum catalytic temperature range of the enzyme activity curve. Such a design realizes the optimal combination of reaction temperature requirements between the reactors in the overall reaction device.

[0038] (5) A gate-type stirring blade is designed in the middle of the fourth-stage enzyme catalytic reactor of the present invention. Since the fourth-stage enzyme catalytic reaction has a long residence time and the reaction tank is relatively large, the purpose of uniform mixing cannot be achieved by relying on the composite enzyme preparation used in the fourth-stage enzyme catalytic reactor. The present invention designs the composite enzyme preparation addition port used in the fourth-stage enzyme catalytic reaction on the third-stage photoenzyme catalytic reactor. Before the end of the third-stage photoenzyme reaction, the composite enzyme preparation used for the fourth-stage catalytic reaction is added to the third-stage photoenzyme catalytic reactor. Since the third-stage photoenzyme catalytic reactor is designed with a vertical stirring structure with excellent stirring, the enzyme preparation is easily mixed evenly in the feed liquid. At this time, the third-stage photoenzyme catalytic reactor becomes the "pre-composite enzyme mixing stirrer" of the fourth-stage enzyme catalytic reactor, and the evenly mixed feed liquid enters the fourth-stage enzyme catalytic reactor. This design avoids the need to set a high-speed stirring device in the large-volume fourth-stage enzyme catalytic reactor, significantly reduces energy consumption, saves costs, and realizes the optimized combination of each reactor in the overall reaction device. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0040] Figure 1 This is a schematic diagram of a complete set of enzyme reaction equipment for enzymatically separating lignocellulose components according to the present invention; Figure 2 This is a front view of the structure of the first-stage enzyme catalytic reactor of the present invention; Figure 3 This is a top view of the first-stage enzyme catalytic reactor structure of the present invention Figure 4 This is a front view of the structure of the second-stage enzyme catalytic reactor of the present invention; Figure 5 A top view of the second-stage enzyme catalytic reactor structure of the present invention Figure 6 Schematic diagram of the structure of the third-stage photoenzyme catalytic reactor of the present invention; Figure 7 This is a schematic structural diagram of the fourth-stage enzyme catalytic reactor of the present invention.

[0041] In the figure: 1, first-stage enzyme-catalyzed reactor; 2, second-stage enzyme-catalyzed reactor; 3, third-stage photoenzyme-catalyzed reactor; 4, fourth-stage enzyme-catalyzed reactor; 1-1, first stirring motor; 1-2, first breathing valve interface; 1-3, first enzyme preparation addition port; 1-4, first pH adjuster addition port; 1-5, first manhole; 1-6, first feed port; 1-7, first enzyme auxiliary agent addition port; 1-8, first reserved port; 1-9, second reserved port; 1-10, first pressure sensor access port; 1-11, first pressure safety valve interface; 1-12, steam interface; 1-13, heating coil; 1-14, first discharge port; 1-15, first stirring blade; 1-16, first pH sensor interface; 1-17, first temperature sensor interface; 1-18, first reactor tank; 1-19, first stirring shaft; 2-1, second manhole; 2-2, second stirring motor; 2-3, second enzyme additive inlet; 2-4, second enzyme preparation inlet; 2-5, second feed inlet; 2-6, second pressure sensor access port; 2-7, third reserved port; 2-8, second breathing valve interface; 2-9, second discharge port; 2-10, second stirring blade; 2-11, cooling jacket; 2-12, second pH sensor interface; 2-13, second temperature sensor interface; 2-14, second stirring shaft; 2-15, second reactor tank; 3-1, third manhole; 3-2, third stirring motor; 3-3, third enzyme additive inlet; 3-4, third enzyme preparation inlet; 3-5, third feed inlet; 3-6, fourth reserved port; 3-7, third pressure sensor access port; 3-8, third breathing valve interface; 3-9, third stirring shaft; 3-10, third discharge port; 3-11, third pH sensor interface; 3-12, third temperature sensor interface; 3-13, light port; 3-14, third reactor tank; 3-15, third stirring blade; 4-1, fourth stirring motor; 4-2, middle shaft screw propeller; 4-3, fourth stirring blade; 4-4, bottom screw propeller; 4-5, fourth discharge port; 4-6, fourth stirring shaft; 4-7 fourth reactor tank. DETAILED DESCRIPTION

[0042] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0043] like Figure 1As shown, a complete enzymatic reaction system for enzymatic hydrolysis and separation of lignocellulose components comprises a first-stage enzymatic reactor 1, a second-stage enzymatic reactor 2, a third-stage photoenzymatic reactor 3, and a fourth-stage enzymatic reactor 4, connected in sequence. The complete system implements a reaction catalysis system centered on the enzyme-photoenzymatic reaction. The first and second-stage enzymatic reactors for the photoenzymatic reaction can be considered the "pretreatment" stage of the third-stage photoenzymatic reactor 3. The fourth-stage enzymatic reactor 4, following the third-stage photoenzymatic reactor 3, serves as the "deep treatment" stage of the enzyme-photoenzymatic reaction, regulating the thoroughness of the catalytic reaction. Based on the structural characteristics of lignocellulose, the specificity of the enzymes, and the specific application scenarios, different complex enzymes are added to the first, second, third, and fourth-stage enzymatic reactors, respectively, to achieve different catalytic reactions according to the material flow path within the reactors. Each reactor can adapt to different sites of enzymatic hydrolysis of lignocellulose components, different application scenarios of the complex enzymes (temperature, pH, etc.), and different physical forms of the material.

[0044] like Figure 2-7 As shown, the first-stage enzyme catalytic reactor 1 is a horizontal reactor with a horizontal stirring structure, the second-stage enzyme catalytic reactor 2 is a horizontal reactor with a vertical stirring structure, and two vertical stirring structures are arranged at both ends of the second-stage enzyme catalytic reactor 2, the third-stage photoenzyme catalytic reactor 3 is a horizontal reactor with a vertical stirring structure, and two vertical stirring structures are arranged at both ends of the second-stage enzyme catalytic reactor 2, and the fourth-stage enzyme catalytic reactor 4 is a vertical reactor with a vertical stirring structure. A central axis screw propeller 4-2 is also provided in the fourth-stage enzymatic hydrolysis reactor, and a bottom screw propeller 4-4 is also provided at the bottom of the fourth-stage enzyme catalytic reactor 4.

[0045] The "complete device" of the present invention is composed of multiple multi-stage enzyme catalytic reactors with stirring forms. Depending on the different properties of the materials, this "complete device" has structures such as horizontal stirring, vertical stirring, and screw propeller transmission. The physical form of the clean straw flakes that have just entered the first-stage enzyme catalytic reactor 1 does not change much, and the material floats on the upper layer. Horizontal stirring is used, and the mechanical power is small, which makes it easy to stir the material into the liquid phase reaction catalytic system. After the previous stage catalytic reaction, the material form changes from hard to soft, and enters the second-stage enzyme catalytic reactor 2 or the third-stage photoenzyme catalytic reactor 3. Vertical stirring is used to achieve rapid stirring, complete catalytic reaction, and reduce raw materials. A screw propeller is used in the larger-volume fourth-stage enzyme catalytic reactor 4 to evenly push out the slurry material after the reaction.

[0046] Specifically, such as Figure 2-3As shown, the horizontal stirring structure of the first-stage enzymatic hydrolysis reactor includes a first stirring shaft 1-19 and a first stirring blade 1-15 arranged on the first stirring shaft 1-19. The first reactor tank body 1-18 of the first-stage enzyme catalytic reactor 1 is a horizontal tank structure. The axis of the first stirring shaft 1-19 is parallel to the axis of the first reactor tank body 1-18. Two groups of first stirring blades 1-15 are symmetrically arranged on the first stirring shaft 1-19 along the midline of the axis of the first reactor tank body 1-18. Preferably, the first stirring blade 1-15 is a spiral stirring blade. The two groups of first stirring blades 1-15 circulate and stir, so that the raw materials are pushed from both ends of the reactor tank body to the center.

[0047] like Figure 4-5 As shown, the vertical stirring structure of the second-stage enzymatic hydrolysis reactor includes a second stirring shaft 2-14 and a second stirring blade 2-10 arranged on the second stirring shaft 2-14. The second reactor tank 2-15 of the second-stage enzymatic reactor 2 is a horizontal tank structure. Two groups of second stirring shafts 2-14 are symmetrically arranged along the midline of the axis of the second reactor tank 2-15. The axis of the second stirring shaft 2-14 is perpendicular to the axis of the second reactor tank 2-15. Each group of second stirring shafts 2-14 is provided with a second stirring blade 2-10. Preferably, the second stirring blade 2-10 is a paddle stirring blade.

[0048] like Figure 6 As shown, the vertical stirring structure of the third-stage photoenzymatic hydrolysis reactor includes a third stirring shaft 3-9 and a third stirring blade 3-15 arranged on the third stirring shaft 3-9. The third reactor tank body 3-14 of the third-stage photoenzymatic catalytic reactor 3 is a horizontal tank body structure. Two groups of third stirring shafts 3-9 are symmetrically arranged along the midline of the axis of the third reactor tank body 3-14. The axis of the third stirring shaft 3-9 is perpendicular to the axis of the third reactor tank body 3-14. Each group of third stirring shafts 3-9 is provided with a third stirring blade 3-15. Preferably, the third stirring blade 3-15 is a paddle-type stirring blade.

[0049] like Figure 7 As shown, the vertical stirring structure of the fourth-stage enzymatic hydrolysis reactor includes a fourth stirring shaft 4-6 and a fourth stirring blade 4-3 arranged on the fourth stirring shaft 4-6. The fourth reactor tank body of the fourth-stage enzymatic reactor 4 is a vertical tank body structure, and a fourth stirring shaft 4-6 parallel to the axis of the fourth reactor tank body is arranged in the middle of the fourth reactor tank body. A fourth stirring blade 4-3 is arranged in the middle of the fourth stirring shaft 4-6. Preferably, the fourth stirring blade 4-3 is a gate-type stirring blade.

[0050] The central-axis propeller 4-2 utilizes a fourth agitator shaft 4-6 as a screw, with a single spiral ribbon disposed in the middle of the fourth agitator shaft 4-6. The bottom propeller 4-4 is driven by a motor to rotate the single spiral ribbon. Because the fourth discharge port 4-5 is located in the middle of the bottom of the fourth reactor vessel, the single spiral ribbons on the bottom propeller 4-4 are symmetrically arranged along the screw's centerline and spiral in opposite directions. After the enzyme reaction, the material is pushed out by the spiral propeller at the bottom of the reactor and pumped into the next stage of the solid-liquid separation process.

[0051] like Figure 1-7 As shown, the first-stage enzyme catalytic reactor 1, the second-stage enzyme catalytic reactor 2, the third-stage photoenzyme catalytic reactor 3 and the fourth-stage enzyme catalytic reactor 4 are all provided with a feed port and a discharge port, and the discharge port of the upper-stage reactor is connected to the feed port of the lower-stage reactor; the first-stage enzyme catalytic reactor 1, the second-stage enzyme catalytic reactor 2 and the third-stage photoenzyme catalytic reactor 3 are all provided with an enzyme preparation addition port, an enzyme auxiliary agent addition port, a pH regulator addition port, a breathing valve interface and a spare port, and are also provided with real-time monitoring elements such as temperature, pressure, pH, and liquid level sensors.

[0052] Specifically, such as Figure 2-3 As shown, the first-stage enzyme catalytic reactor 1 is provided with a first enzyme preparation addition port 1-3, a first enzyme auxiliary agent addition port 1-7, a first pH regulator addition port 1-4 and a first breathing valve interface 1-2; the second-stage enzyme catalytic reactor 2 is provided with a second enzyme preparation addition port 2-4, a second enzyme auxiliary agent addition port 2-3 and a second breathing valve interface 2-8; the third-stage photoenzyme catalytic reactor 3 is provided with a third enzyme preparation addition port 3-4, a third enzyme auxiliary agent addition port 3-3 and a third breathing valve interface 3-8.

[0053] Specifically, such as Figure 2-3As shown, in the first-stage enzyme catalytic reactor 1, the first-stage enzyme catalytic reactor 1 is provided with a steam interface 1-12 with a muffler, and the first-stage enzyme catalytic reactor 1 is provided with an auxiliary heating coil 1-13, and the outside of the first-stage enzyme catalytic reactor 1 is provided with a thermal insulation layer; its stirring shaft is connected to the first stirring motor 1-1, the first feed port 1-6 is provided at the top of the first reactor tank body, the first feed port 1-6 is provided with a valve, the first discharge port 1-14 is provided at the bottom of the reactor tank body, the discharge port is also provided with a valve, the steam interface 1-12 with a muffler is provided at the first reactor The bottom side of the tank body is provided with multiple steam interfaces 1-12 with silencers, the first breathing valve interface 1-2 is provided on the top of the first reactor tank body, and the first breathing valve interface 1-2 is provided with a breathing regulating valve; the first reactor tank body is also provided with a first pressure sensor access port 1-10, a first pH sensor interface 1-16 and a first temperature sensor interface 1-17 for installing a pressure sensor, a pH sensor and a temperature sensor respectively; a first manhole 1-5, a first pressure safety valve interface 1-11 and a first reserved port 1-8 and a second reserved port 1-9 are also provided as spare interfaces.

[0054] like Figure 4-5 As shown, in the second-stage enzyme catalytic reactor 2, the second-stage enzyme catalytic reactor 2 is provided with a cooling jacket 2-11, the second stirring shaft is connected to the second stirring motor 2-2, the second feed port 2-5 is arranged at the top of the second reactor tank body, and the second feed port 2-5 is provided with a valve closer switch feed port, the second discharge port 2-9 is arranged at the bottom of the second reactor tank body, and the second discharge port 2-9 is also provided with a valve, the breathing valve interface is arranged at the top of the second reactor tank body, and the second breathing valve interface 2-8 is provided with a breathing regulating valve; the second reactor tank body is also provided with a second pressure sensor access port 2-6, a second pH sensor interface 2-12 and a second temperature sensor interface 2-13 for installing a pressure sensor, a pH sensor and a temperature sensor respectively; a second manhole 2-1 and a third reserved port 2-7 are also provided.

[0055] like Figure 6As shown, in the third-stage photoenzyme catalytic reactor 3, the third-stage photoenzyme catalytic reactor 3 is evenly distributed with multiple light ports 3-13, and the multiple light ports 3-13 are sealed with glass sheets with good light transmittance. An external light source of a certain intensity irradiates the photoenzyme catalytic reaction system through the light ports 3-13, the third stirring shaft is connected to the third stirring motor 3-2, the third feed port 3-5 is arranged at the top of the third reactor tank body, and the third feed port 3-5 is provided with a valve, the third discharge port 3-10 is arranged at the bottom of the third reactor tank body, and the third discharge port 3-10 is also provided with a valve, the third breathing valve interface 3-8 is arranged at the top of the reactor tank body, and the third breathing valve interface 3-8 is provided with a breathing regulating valve; the third reactor tank body is also provided with a third pressure sensor access port 3-7, a third pH sensor interface 3-11 and a third temperature sensor interface 3-12 for installing a pressure sensor, a pH sensor and a temperature sensor respectively; a third manhole 3-1 and a fourth reserved port 3-6 are also provided.

[0056] like Figure 7 As shown, in the fourth-stage enzyme catalytic reactor 4, the outside of the fourth-stage enzyme catalytic reactor 4 is provided with an insulation layer, the fourth stirring shaft is connected to the fourth stirring motor 4-1, the fourth feed port is provided at the top of the fourth reactor tank body, and a valve is provided on the fourth feed port, and the fourth discharge port 4-5 is provided at the bottom of the fourth reactor tank body, and a valve is also provided on the fourth discharge port 4-5.

[0057] A further technical solution of the present invention is that the volume of the second-stage enzyme catalytic reactor 2 and the third-stage photoenzyme catalytic reactor 3 is 70%-80% of the volume of the first-stage enzyme catalytic reactor 1, and the volume of the fourth-stage enzyme catalytic reactor 4 is 3-5 times the volume of the first-stage enzyme catalytic reactor 1. In the second-stage enzyme catalytic reactor and the third-stage photoenzyme catalytic reactor, since the raw material softens and enters the liquid phase catalytic volume, the "volume" becomes smaller than that in the first-stage enzyme catalytic reactor. Therefore, the volume of the enzyme catalytic reactors provided in the second and third stages is reduced, its manufacturing cost is reduced, and it is also conducive to uniform stirring. The fourth-stage enzyme catalytic reaction time is 3-5 times that of the first-stage enzyme catalytic reaction time. Therefore, the volume of the fourth-stage enzyme catalytic reactor is 3-5 times the volume of the first-stage enzyme catalytic reactor, which can realize continuous production of the complete set of equipment.

[0058] A process for enzymatically separating lignocellulose components using an enzyme reaction complete set of equipment comprises the following steps: S1. The cleaned straw pieces are fed into the first-stage enzyme reactor, and water is added according to a solid-liquid ratio of 1:5-8 to form a reaction system; the stirring speed is 5-15 rpm, and the stirring mode is circulating stirring. The corresponding complex enzyme preparation, enzyme additive, and pH adjuster are added to the first-stage enzyme reactor; high-temperature steam is introduced into the first-stage enzyme reactor to heat the material to the optimal enzyme activity temperature of the first-stage enzyme catalytic reaction; the temperature, pressure, pH, liquid level and other sensors on the first-stage enzyme catalytic reaction are used to feedback and adjust the enzyme reaction to maintain the optimal conditions until the first-stage enzyme catalytic reaction is completed; S2. After the reaction in the first-stage enzyme-catalyzed reactor is completed, the material enters the second-stage enzyme-catalyzed reactor with a stirring speed of 50-150 rpm and a stirring mode of stirring in the same direction, intermittent forward and reverse directions; the corresponding complex enzyme preparation, enzyme aid, and pH regulator are added to the second-stage enzyme-catalyzed reactor; the jacket circulating water is cooled to the optimal enzyme activity temperature of 85-110°C for the second-stage enzyme-catalyzed reaction; the temperature, pressure, pH, liquid level and other sensors on the second-stage enzyme-catalyzed reactor are feedback-regulated to maintain the optimal enzyme reaction conditions until the second-stage enzyme-catalyzed reaction is completed; in S2, 10 minutes before the end of the second-stage enzyme-catalyzed reaction, the material is further cooled to the optimal enzyme activity reaction temperature of 65-75°C for the third-stage photoenzyme-catalyzed reaction; S3. After the reaction in the second-stage enzyme-catalyzed reactor is completed, the material enters the third-stage photoenzyme-catalyzed reactor with a stirring speed of 50-150 rpm and a stirring mode of stirring in the same direction, intermittent forward and reverse directions; the corresponding complex enzyme preparation, enzyme aid, and pH regulator are added to the third-stage photoenzyme-catalyzed reactor; the light port on the reactor tank is irradiated under certain light intensity conditions of an external light source; the temperature, pressure, pH, liquid level and other sensors on the reactor tank are used to adjust the feedback to maintain the optimal conditions for the enzyme reaction until the third-stage photoenzyme-catalyzed reaction is completed; S4. After the reaction in the third-stage photoenzyme catalytic reactor is completed, the material enters the fourth-stage enzyme catalytic reactor with a stirring speed of 20-60 rpm and a circulating stirring method. The temperature is maintained at 40-60°C using a natural trend cooling method. After the fourth-stage enzyme catalytic reaction is completed, the material is pumped into the next stage of solid-liquid separation equipment.

[0059] Preferably, in S1, the size of the clean straw flakes after processing and cleaning is 2-3 cm.

[0060] Preferably, in S2, when high-temperature steam is introduced into the first-stage enzyme catalytic reactor to heat the material, synergistic heating is performed through the heating coil.

[0061] Preferably, in S3, 5 minutes before the completion of the third-stage photoenzyme catalysis, the complex enzyme preparation required for the fourth-stage catalytic reaction is added.

[0062] Preferably, in S4, the material is delivered out through the middle axis screw propeller and the bottom screw propeller after the fourth stage enzyme catalysis reaction is completed.

[0063] Example S1. After weighing clean 2-3 cm straw flakes online, a certain weight of straw material is fed into the first-stage enzyme catalytic reactor 1 through the feed port at the upper end of the reactor, forming a reaction system with a solid-liquid ratio of 1:6. The reactor is a horizontal tank structure with a stirring shaft parallel to the tank body, a stirring speed of 10 rpm, and a stirring blade direction that pushes the raw materials from both ends toward the center for circulatory stirring. An insulation layer is added to the outside. The corresponding complex enzyme preparation, enzyme auxiliary agent, and pH adjuster are added through different inlets on the reactor tank body. Simultaneously, auxiliary heating is provided by a steam direct heater with a muffler and a coil on the reactor, and a breathing valve is adjusted to achieve the application scenario of the enzyme catalytic reaction (temperature 95°C, pH 8.5). The temperature, pressure, pH, and liquid level sensors on the reactor are used for real-time monitoring. The enzymatic hydrolysis is stirred for 2 hours, and the enzyme catalytic reaction is adjusted to complete the first stage. S2. After the completion of the first-stage enzymatic hydrolysis reaction, the material enters the second-stage enzymatic reactor 2 and the feed valve is closed; the stirring speed of the vertical stirring structure in the second-stage enzymatic reactor is 110 rpm, and the total power of the two first stirring motors is 20KW / h. If a vertical reactor with a vertical stirring structure is used, its power is 45KW / h; the volume is 80% of the volume of the first-stage enzymatic reactor 1; the outside is a jacketed insulation layer; the lower end has a discharge port and a valve; the cooling control of the second-stage enzymatic reactor is completed by a circulating water system consisting of a tank jacket, cooled to 85°C, and the pH is natural; the reactor has an enzyme preparation addition port, an additive addition port, a breathing regulating valve and a spare port; the reactor has real-time monitoring components such as temperature, pressure, pH, and liquid level sensors; the enzymatic hydrolysis is stirred for 1.5 hours, and 10 minutes before the end of the second-stage enzymatic reaction, the material is further cooled to the enzyme activity reaction temperature of the third-stage photoenzyme catalytic reaction of 75°C; S3. After entering the third-stage photoenzyme catalytic reactor 3 through the discharge port of the second-stage enzyme catalytic reactor 2 and the feed port connected to the third-stage photoenzyme catalytic reactor 3, the feed port valve is closed; the reactor is a horizontal tank structure with vertical stirring structures at both ends, the stirring speed is 100 rpm, and the total power of the two first stirring motors is 20KW / h. If a vertical reactor with a vertical stirring structure is used, its power is 45KW / h; the volume is the same as that of the secondary enzyme catalytic reactor; there is a light port 3-13 sealed with good light transmittance glass on the tank body of the reactor, and light of a certain wavelength is irradiated through the light port 3-13 to cause a photoenzyme catalytic reaction in the reactor, and the light intensity is 45mW / cm 2The reactor is equipped with an enzyme preparation addition port, an auxiliary agent addition port, a breathing regulating valve, and a spare port. It is also equipped with real-time monitoring components such as temperature, pressure, pH, and liquid level sensors. The stirring enzymolysis time is 1.5 hours. 5 minutes before the completion of the third-stage photoenzyme catalysis, the complex enzyme preparation required for the fourth-stage catalytic reaction is added. This takes on the function of mixing the complex enzyme preparation evenly in the fourth-stage catalytic reactor, overcoming the problem that the fourth-stage enzyme catalytic reactor, which is large in size and requires a high-power stirrer, is also a problem. S4. 5 minutes before the completion of the third-stage photoenzyme catalysis, the complex enzyme preparation required for the fourth-stage catalytic reaction is added. Because the third-stage photoenzyme catalytic reactor 3 is designed with a vertical stirring structure with excellent stirring, the enzyme preparation is easily mixed with the feed liquid. At this time, the third-stage photoenzyme reactor becomes the "pre-complex enzyme mixing and stirring device" of the fourth-stage enzyme catalytic reactor 4. The evenly mixed feed liquid enters the fourth-stage enzyme catalytic reactor 4 through the feed port connected to the third-stage photoenzyme catalytic reactor 3. After entering the fourth-stage enzyme catalytic reactor 4 through the feed port, the feed port valve is closed and the stirring speed is 20 rpm. The reactor tank is a vertical reactor tank structure with a volume three times that of the first-stage enzymatic hydrolysis reactor. There is a spiral propeller in the middle, a fourth stirring blade at the bottom, a spiral propeller perpendicular to the tank body at the bottom, and an insulation layer added on the outside. The enzymatic hydrolysis reaction lasts for 6 hours. After the material completes the fourth-stage enzymatic reaction, the spiral propeller at the bottom of the reactor pushes the material out and is pumped into the next stage of solid-liquid separation equipment.

[0064] Compared with the prior art, this embodiment has substantially the same material properties of the intermediate product and the final product when other process steps and parameters are the same, but the power of the second-stage enzymatic hydrolysis reactor and the third-stage photoenzymatic hydrolysis reactor is reduced by about 50%, and the overall enzyme reaction time is reduced by about 25%.

[0065] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the invention is defined by the appended claims, not the foregoing description, and all variations within the meaning and range of equivalents of the claims are intended to be included therein. Any reference sign in a claim should not be construed as limiting the claim to which it relates.

Claims

1. A complete set of enzyme reaction equipment for enzymatic hydrolysis and separation of lignocellulose components, characterized by: It comprises a first-stage enzyme catalytic reactor (1), a second-stage enzyme catalytic reactor (2), a third-stage photoenzyme catalytic reactor (3) and a fourth-stage enzyme catalytic reactor (4) which are connected in sequence; The first-stage enzyme catalytic reactor (1) is a horizontal reactor with a horizontal stirring structure, the second-stage enzyme catalytic reactor (2) is a horizontal reactor with a vertical stirring structure, and two vertical stirring structures are arranged at both ends of the second-stage enzyme catalytic reactor (2), the third-stage photoenzyme catalytic reactor (3) is a horizontal reactor with a vertical stirring structure, and two vertical stirring structures are arranged at both ends of the second-stage enzyme catalytic reactor (2), and the fourth-stage enzyme catalytic reactor (4) is a vertical reactor with a vertical stirring structure; the fourth-stage enzyme catalytic reactor (4) is also provided with a central axis screw propeller (4-2) and a bottom screw propeller (4-4); The first-stage enzyme catalytic reactor (1), the second-stage enzyme catalytic reactor (2), the third-stage photoenzyme catalytic reactor (3) and the fourth-stage enzyme catalytic reactor (4) are all provided with a feed port and a discharge port, and the discharge port of the upper-stage reactor is connected to the feed port of the lower-stage reactor; the first-stage enzyme catalytic reactor (1), the second-stage enzyme catalytic reactor (2) and the third-stage photoenzyme catalytic reactor (3) are all provided with an enzyme preparation addition port, an enzyme auxiliary agent addition port, a pH regulator addition port, a breathing valve interface and a spare port, and are also provided with a temperature sensor, a pressure sensor, a pH sensor and a liquid level sensor; The first-stage enzyme catalytic reactor (1) is provided with a steam heating device and an auxiliary heating device; the second-stage enzyme catalytic reactor (2) is provided with a cooling device; the third-stage photoenzyme catalytic reactor (3) is provided with a plurality of light ports (3-13) uniformly distributed; The volumes of the second-stage enzyme catalytic reactor (2) and the third-stage photoenzyme catalytic reactor (3) are 70%-80% of the volume of the first-stage enzyme catalytic reactor (1), and the volume of the fourth-stage enzyme catalytic reactor (4) is 3-5 times the volume of the first-stage enzyme catalytic reactor (1).

2. The complete enzyme reaction device for enzymatic hydrolysis and separation of lignocellulose components according to claim 1, characterized in that: The horizontal stirring structure of the first-stage enzyme catalytic reactor (1) comprises a first stirring shaft (1-19) and a first stirring blade (1-15), and two groups of first stirring blades (1-15) are symmetrically arranged on the first stirring shaft (1-19) along the center line of the axis of the first reactor tank (1-18); the vertical stirring structure of the second-stage enzyme catalytic reactor (2) comprises a second stirring shaft (2-14) and a second stirring blade (2-10), and two groups of second stirring shafts (2-14) are symmetrically arranged along the center line of the axis of the second reactor tank (2-15), and each group of second stirring shafts (2-14) is provided with There is a second stirring blade (2-10); the vertical stirring structure of the third-stage photoenzyme catalytic reactor (3) (3) includes a third stirring shaft (3-9) and a third stirring blade (3-15), and two groups of third stirring shafts (3-9) are symmetrically arranged along the center line of the axis of the third reactor tank (3-14), and each group of third stirring shafts (3-9) is provided with a third stirring blade (3-15); the vertical stirring structure of the fourth-stage enzyme catalytic reactor (4) includes a fourth stirring shaft (4-6) and a fourth stirring blade (4-3), and the fourth stirring shaft (4-6) is provided with a fourth stirring blade (4-3).

3. The complete enzyme reaction device for enzymatic hydrolysis and separation of lignocellulose components according to claim 2, characterized in that: The steam heating device is a steam generator, which is connected to the first reactor tank (1-18) through a steam interface (1-12); the auxiliary heating device is a heating coil (1-13), and the heating coil (1-13) is evenly distributed on the outer wall of the first reactor tank (1-18); the cooling device is a jacket, which is arranged on the outer wall of the second reactor tank (2-15).

4. The complete enzyme reaction device for enzymatic hydrolysis and separation of lignocellulose components according to claim 2, characterized in that: The first stirring blade (1-15) is a spiral stirring blade, the second stirring blade (2-10) is a paddle stirring blade, the third stirring blade (3-15) is a paddle stirring blade, and the fourth stirring blade (4-3) is a gate stirring blade.

5. The complete enzyme reaction device for enzymatic hydrolysis and separation of lignocellulose components according to claim 1, characterized in that: The first-stage enzyme catalytic reactor (1), the second enzyme catalytic reactor (2) and the fourth-stage enzyme catalytic reactor (4) are all provided with insulation layers on their exteriors.

6. The complete enzymatic reaction device for enzymatic hydrolysis and separation of lignocellulose components according to any one of claims 1 to 5, characterized in that: The first stirring shaft (1-19) of the first-stage enzyme catalytic reactor (1) is connected to the first stirring motor (1-1), the first feed port (1-6) is arranged at the top of the first reactor tank (1-18), the first discharge port (1-14) is arranged at the bottom of the first reactor tank (1-18), valves are arranged on the first feed port (1-6) and the first discharge port (1-14), a steam interface (1-12) with a muffler is arranged at the bottom side of the reactor tank, a plurality of steam interfaces (1-12) with a muffler are arranged, and a first breathing valve interface (1-2) is arranged A breathing regulating valve is provided on the first breathing valve interface (1-2) at the top of the first reactor tank body (1-18); a first pressure sensor access port (1-10), a first pH sensor interface (1-16) and a first temperature sensor interface (1-17) are also provided on the first reactor tank body (1-18) for installing a pressure sensor, a pH sensor and a temperature sensor respectively; a first manhole (1-5), a first pressure safety valve interface (1-11) and a first reserved port (1-8) and a second reserved port (1-9) are also provided on the first reactor tank body (1-18).

7. The complete enzymatic reaction device for enzymatic hydrolysis and separation of lignocellulose components according to any one of claims 1 to 5, characterized in that: The second stirring shaft (2-14) in the second-stage enzyme catalytic reactor (2) is connected to the second stirring motor (2-2), the second feed port (2-5) is arranged at the top of the second reactor tank (2-15), the second discharge port (2-9) is arranged at the bottom of the second reactor tank (2-15), the second feed port (2-5) and the second discharge port (2-9) are both provided with valves, the second breathing valve interface (2-8) is arranged at the top of the second reactor tank (2-15), and the second breathing valve interface (2-8) is provided with a breathing regulating valve; the second reactor tank (2-15) is also provided with a second pressure sensor access port (2-6), a second pH sensor interface (2-12) and a second temperature sensor interface (2-13) for installing a pressure sensor, a pH sensor and a temperature sensor respectively; the second reactor tank (2-15) is also provided with a second manhole (2-1) and a third reserved port (2-7).

8. The complete enzyme reaction device for enzymatic hydrolysis and separation of lignocellulose components according to any one of claims 1 to 5, characterized in that: The third stirring shaft (3-9) of the third-stage photoenzyme catalytic reactor (3) is connected to the third stirring motor (3-2); the third feed port (3-5) is arranged at the top of the third reactor tank (3-14) and is provided with a valve; the third discharge port (3-10) is arranged at the bottom of the third reactor tank (3-14); the third feed port (3-5) and the third discharge port (3-10) are both provided with valves; the third breathing valve interface (3-8) is arranged at the top of the third reactor tank (3-14); the third breathing valve interface (3-8) is provided with a breathing regulating valve; the third reactor tank (3-14) is also provided with a third pressure sensor access port (3-7), a third pH sensor interface (3-11) and a third temperature sensor interface (3-12) for installing a pressure sensor, a pH sensor and a temperature sensor respectively; the third reactor tank (3-14) is also provided with a third manhole (3-1) and a fourth reserved port (3-6).

9. The complete enzyme reaction device for enzymatic hydrolysis and separation of lignocellulose components according to any one of claims 1 to 5, characterized in that: The fourth feed port of the fourth-stage enzyme catalytic reactor (4) is arranged at the top of the fourth reactor tank (4-7), and the fourth discharge port (4-5) is arranged at the bottom of the fourth reactor tank (4-7). Valves are provided on both the fourth feed port and the fourth discharge port (4-5).

10. A lignocellulose component enzymatic reaction process using the complete enzymatic reaction device for enzymatically decomposing and separating lignocellulose components according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1. The cleaned straw flakes are fed into the first-stage enzyme catalytic reactor (1), and water is added at a solid-liquid ratio of 1:5-8 to form a reaction system; the stirring speed is 5-15 rpm, and the stirring mode is circulating stirring, and the composite enzyme preparation, enzyme auxiliary agent, and pH regulator are added to the first-stage enzyme catalytic reactor (1); the materials in the first-stage enzyme catalytic reactor (1) are heated by a steam device to an enzyme activity temperature of 85-110°C for the first-stage enzyme catalytic reaction; the enzyme reaction conditions are maintained by feedback adjustment through the temperature sensor, pressure sensor, pH sensor, and liquid level sensor on the first reactor tank until the first-stage enzyme catalytic reaction is completed; S2. After the reaction in the first-stage enzyme catalytic reactor (1) is completed, the material enters the second-stage enzyme catalytic reactor (2) with a stirring speed of 50-150 rpm and a stirring mode of stirring in the same direction, intermittent forward and reverse directions; a composite enzyme preparation, an enzyme auxiliary agent, and a pH regulator are added to the second-stage enzyme catalytic reactor (2); the material is cooled to the enzyme activity temperature of 80-90°C in the second-stage enzyme catalytic reaction through a cooling device; the enzyme reaction conditions are maintained through feedback adjustment by the temperature sensor, pressure sensor, pH sensor, and liquid level sensor on the second reactor tank. 10 minutes before the end of the second-stage enzyme catalytic reaction, the material is further cooled to the enzyme activity temperature of 65-75°C in the third-stage photoenzyme catalytic reaction; S3. After the reaction in the second-stage enzyme catalytic reactor (2) is completed, the material enters the third-stage photoenzyme catalytic reactor (3) with a stirring speed of 50-150 rpm and a stirring mode of stirring in the same direction, intermittent forward and reverse directions; a composite enzyme preparation, an enzyme auxiliary agent, and a pH regulator are added to the third-stage photoenzyme catalytic reactor (3); an external light source is used to illuminate the light port (3-13) on the reactor tank; and the enzyme reaction conditions are maintained by feedback adjustment through the temperature sensor, pressure sensor, pH sensor, and liquid level sensor on the third reactor tank until the third-stage photoenzyme catalytic reaction is completed; S4. After the reaction in the third-stage photoenzyme catalytic reactor (3) is completed, the material enters the fourth-stage enzyme catalytic reactor (4) with a stirring speed of 20-60 rpm. The temperature is maintained at 40-60°C by adopting a natural trend cooling method. After the fourth-stage enzyme catalytic reaction is completed, the material is pumped into the next stage of solid-liquid separation equipment.

11. The process according to claim 10, characterized in that: In S2, when the steam device is introduced into the first-stage enzyme catalytic reactor (1) to heat the material, the auxiliary heating device is used for synergistic heating.

12. The process according to claim 10, characterized in that: In S3, 5 minutes before the completion of the third-stage photoenzyme catalysis, the complex enzyme preparation required for the fourth-stage catalytic reaction is added and stirred at a stirring speed of 50-150 rpm.

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