Accurate temperature gradient regulation and control method and device

Abstract

The invention discloses a precise temperature gradient regulation and control method and device, and belongs to the field of microalgae cultivation. A precise temperature gradient regulation and control device comprises a transparent cultivation tank, a stirring shaft is rotationally connected into the cultivation tank, a plurality of sets of stirring paddles are installed on the stirring shaft at equal intervals, at least one temperature sensor is arranged on the stirring paddles on the same plane, and a driving part for driving the stirring shaft to rotate is arranged on the cultivation tank; the stirring shaft rotates at a low speed to stir microalgae in the culture tank to move, so that the microalgae are kept in a suspended state and receive uniform illumination, and the culture effect of the microalgae is improved; meanwhile, through cooperation of a reciprocating lead screw, a magnetic suction plate and a piston plate, air can be continuously inflated into cultivation liquid in the cultivation tank, and through cooperation of stirring and the upward floating effect of airflow, the microalgae can make uniform contact with carbon dioxide in the air, the uniform respiration effect is achieved, and the cultivation effect of the microalgae is further improved.

Description

Technical Field

[0001] The present invention relates to the technical field of microalgae cultivation, and in particular to a method and device for precise temperature gradient control. Background Art

[0002] Microalgae are a group of small algae with efficient photosynthesis and rapid growth. Through reasonable cultivation techniques, the yield and quality of microalgae can be significantly improved, thereby enhancing its value as a biological resource. However, the growth of microalgae is affected by many factors, including light, nutrient concentration, pH value and temperature. Among these, temperature is considered to be one of the most important environmental factors affecting the growth of microalgae. Different microalgae species and different growth stages have their own optimal growth temperature range. Exceeding this range may lead to growth stagnation or cell death.

[0003] At present, the existing temperature control method is to maintain the set target temperature by adjusting the working state of the heating or cooling equipment. During the temperature control, the microalgae liquid near the temperature conducting part will be exposed to the temperature control effect first, while the microalgae liquid far away from the temperature conducting part will be exposed to the temperature control effect with a delay. This makes the temperature control uniformity insufficient, and the microalgae will precipitate and accumulate during the cultivation process, making it impossible for them to evenly receive nutrients and light, ultimately resulting in a reduction in the microalgae cultivation effect. Therefore, a precise temperature gradient control method and device are proposed. Summary of the Invention

[0004] The purpose of the present invention is to solve the problem of insufficient temperature uniformity and the inability of microalgae to uniformly receive nutrients and light, which ultimately leads to reduced microalgae cultivation effect, and to propose a precise temperature gradient control method and device.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions: A precise temperature gradient control method comprises the following steps: Step 1: Obtain the temperature inside the microalgae cultivation device; Step 2: Calculate the difference between the temperature obtained in step 1 and the temperature required for the actual growth stage of the microalgae; Step 3: Determine whether the temperature in the cultivation device meets the requirements of the microalgae growth stage at this time through the difference; Step 4: When the temperature difference exceeds the set cultivation threshold, the temperature in the cultivation device is adjusted in real time.

[0006] A precise temperature gradient control device comprises a transparent cultivation tank, wherein a stirring shaft is rotatably connected inside the cultivation tank, a plurality of stirring paddles are installed on the stirring shaft at equal intervals, at least one temperature sensor is provided on the stirring paddles on the same plane, and the cultivation tank is provided with a driving part for driving the stirring shaft to rotate, and further comprises: a piston cylinder, wherein the piston cylinder is fixed to the cultivation tank, wherein a temperature control chamber is fixedly connected to the bottom of the piston cylinder, and a temperature control part for heating the airflow in the piston cylinder is provided on the temperature control chamber, and a guide part for pushing the heated airflow into the interior of the cultivation tank is provided in the piston cylinder.

[0007] In order to improve the cultivation effect, preferably, the driving part includes a mounting bracket, the mounting bracket is fixed on the top of the cultivation tank, the top of the mounting bracket is fixedly connected to a driving motor, the bottom of the mounting bracket is fixedly connected to a reducer, the output shaft of the driving motor is connected to the input end of the reducer, and the top end of the stirring shaft is connected to the output end of the reducer.

[0008] In order to facilitate temperature control, preferably, the temperature control part includes a medium tube, the medium tube is fixed in the temperature control chamber, and the medium tube is arranged in a serpentine shape. The side wall of the temperature control chamber is fixedly connected to a temperature controller, and the output end of the temperature controller is connected to the end of the medium tube.

[0009] In order to improve the uniformity of temperature control, preferably, a guide groove is provided inside the stirring paddle, a guide channel is provided inside the stirring shaft, the guide groove is connected to the guide channel, an air guide ring is rotatably connected to the outer wall of the stirring shaft, the top of the air guide ring is fixedly connected to the top of the cultivation tank, and the top of the guide channel is connected to the inner cavity of the air guide ring, the guide groove facing the back force surface of the stirring paddle is connected to the inner cavity of the cultivation tank, the back force surface of the stirring paddle is fixedly connected to an ultrafiltration membrane, and the ultrafiltration membrane covers the connection between the guide groove and the cultivation tank.

[0010] Furthermore, the guide part includes a piston plate, which is slidably connected in the piston cylinder, and a magnetic plate is slidably sleeved on the outer wall of the piston cylinder, and the magnetic plate and the piston plate are magnetically attracted to each other. A reciprocating screw is rotatably connected between the top of the incubation tank and the top of the temperature control chamber, and a lifting plate is threadedly sleeved on the reciprocating screw. The side wall of the lifting plate is fixedly connected to the side wall of the magnetic plate, and the top of the reciprocating screw is connected to the stirring shaft through a pulley group. The bottom end of the piston cylinder is connected to the temperature control chamber, and the side wall of the temperature control chamber is fixed and connected to an inflation tube. The other end of the inflation tube is connected to the inner cavity of the air guide ring, and a one-way valve is provided in the inflation tube.

[0011] In order to improve the temperature regulation effect, preferably, the lower part of the side wall of the piston cylinder is fixed and connected with an exhaust pipe, the other end of the exhaust pipe is connected to the top of the inner cavity of the cultivation tank, and an air guide pipe is fixed and connected on the top side wall of the cultivation tank, and a one-way valve is provided in the exhaust pipe.

[0012] In order to improve the neatness of the cultivation tank, preferably, a sealing ring is fixedly connected to the upper part of the side wall of the cultivation tank, the sealing ring is connected to the side facing the cultivation tank, and a rotating ring is slidably connected inside the sealing ring, two groups of scraping rods are symmetrically fixed on the side of the rotating ring facing the center of the cultivation tank, and multiple groups of windward blades are fixed at equal intervals on the side of the rotating ring located inside the sealing ring, and a thrust tube is fixed to and connected to the upper part of the side wall of the piston cylinder, the other end of the thrust tube is connected to the inner cavity of the sealing ring, and the connecting port of the thrust tube is inclined toward the windward blade.

[0013] Preferably, both sides of the top of the cultivation tank are fixed and connected with addition pipes, the top of the addition pipes is fixedly connected with a sealing cover, the bottom of the cultivation tank is fixedly connected with a discharge pipe, and a solenoid valve is provided in the discharge pipe.

[0014] In order to achieve precise temperature control, preferably, a PLC controller is fixedly connected to the top of the cultivation tank, and the temperature controller, temperature sensor and PLC controller are electrically connected.

[0015] Compared with the prior art, the present invention provides a method and device for precise temperature gradient control, which has the following beneficial effects: 1. This precise temperature gradient control device moves the microalgae in the cultivation tank through the slow rotation of the stirring shaft, keeping them in a suspended state and receiving uniform light, thereby improving the cultivation effect of the microalgae. At the same time, the reciprocating screw, magnetic plate, and piston plate cooperate to continuously fill the cultivation liquid in the cultivation tank with air. Combined with the above-mentioned stirring and the upward floating effect of the airflow, the microalgae are evenly exposed to carbon dioxide in the air, achieving uniform respiration, further improving the cultivation effect of the microalgae.

[0016] 2. This precise temperature gradient control device uses the airflow thrust generated by the piston plate sliding upward in the piston cylinder to push the rotating ring and the scraper rod to slowly rotate along the inner wall of the cultivation tank, thereby cleaning the inner wall of the cultivation tank, improving the cleanliness of the inner wall of the cultivation tank, and reducing the obstruction of the transparent inner wall of the cultivation tank by microalgae, so that management personnel can observe the microalgae being cultivated.

[0017] 3. The precise temperature gradient control device realizes precise control of the temperature in the cultivation tank through the linkage of temperature sensors, temperature controllers and PLC controllers. The heating or cooling effect generated by the temperature controller gradually increases, and the temperature in the cultivation tank is gradually changed through the continuous flow of air, avoiding the physiological stress of microalgae caused by rapid temperature changes, thereby ensuring the cultivation effect of microalgae. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the overall structure of a precise temperature gradient control device proposed by the present invention. Figure 1 ; Figure 2 This is a schematic diagram of the overall structure of a precise temperature gradient control device proposed by the present invention. Figure 2 ; Figure 3 This is a schematic diagram of the partial cross-sectional structure of a precise temperature gradient control device proposed by the present invention; Figure 4 A precise temperature gradient control device proposed by the present invention Figure 3 Schematic diagram of the enlarged structure of area A in the middle; Figure 5 This is a schematic diagram of the internal structure of a sealing ring of a precise temperature gradient control device proposed by the present invention; Figure 6 This is a schematic diagram of the partial cross-sectional structure of the stirring shaft of a precise temperature gradient control device proposed in the present invention; Figure 7 A precise temperature gradient control device proposed by the present invention Figure 6 Schematic diagram of the enlarged structure of the middle B area; Figure 8 This is a flow chart of a precise temperature gradient control method proposed in the present invention.

[0019] In the figure: 1. Cultivation tank; 2. Stirring shaft; 21. Stirring paddle; 22. Temperature sensor; 3. Piston cylinder; 31. Temperature control chamber; 4. Mounting frame; 41. Drive motor; 42. Reducer; 5. Medium pipe; 51. Temperature controller; 6. Guide trough; 61. Guide channel; 62. Air guide ring; 63. Ultrafiltration membrane; 7. Piston plate; 71. Magnetic plate; 72. Reciprocating screw; 721. Lifting plate; 722. Pulley assembly; 73. Inflating pipe; 74. Exhaust pipe; 75. Air guide pipe; 8. Sealing ring; 81. Rotating ring; 82. Scraper rod; 83. Windward blade; 84. Thrust pipe; 9. Adding pipe; 91. Discharge pipe; 92. PLC controller. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0021] In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as limiting the present invention.

[0022] Example 1: Reference Figure 8 , a precise temperature gradient control method, comprising the following steps: Step 1: Obtain the temperature inside the microalgae cultivation device; The real-time temperature of the microalgae cultivation liquid in the cultivation tank 1 is measured by using multiple groups of temperature sensors 22 arranged at different heights in the cultivation tank 1 , and the temperature data is transmitted to the PLC controller 92 .

[0023] Step 2: Calculate the difference between the temperature obtained in step 1 and the temperature required for the actual growth stage of the microalgae; In the PLC controller 92 , the obtained temperature is compared with the temperature required for the actual growth stage of the microalgae, and the difference between the two is obtained.

[0024] Step 3: Determine whether the temperature in the cultivation device meets the requirements of the microalgae growth stage at this time through the difference; When the difference between the two exceeds the threshold range, the PLC controller 92 will send a temperature increase or temperature decrease instruction to the temperature controller 51.

[0025] Step 4: When the temperature difference exceeds the set cultivation threshold, the temperature in the cultivation device is adjusted in real time; After receiving the corresponding temperature adjustment instruction, the temperature controller 51 will heat or cool the liquid medium in the medium tube 5. When the air flow passes through the temperature control chamber 31 and enters the cultivation tank 1, it will be heated or cooled, thereby realizing real-time regulation of the temperature of the cultivation liquid in the cultivation tank 1.

[0026] Example 2: Reference Figures 1-8In order to implement the control method in Example 1, a precise temperature gradient control device is proposed, comprising a transparent cultivation tank 1, wherein both sides of the top of the cultivation tank 1 are fixed and connected with an addition pipe 9, the top of the addition pipe 9 is fixedly connected to a sealing cover, the bottom of the cultivation tank 1 is fixedly connected to a discharge pipe 91, and an electromagnetic valve is provided in the discharge pipe 91, a stirring shaft 2 is rotatably connected in the cultivation tank 1, a plurality of groups of stirring paddles 21 are evenly installed on the stirring shaft 2, at least one temperature sensor 22 is provided on the stirring paddles 21 on the same plane, and the cultivation tank 1 is provided with a driving part for driving the stirring shaft 2 to rotate, and further comprising: a piston cylinder 3, the piston cylinder 3 is fixed to the cultivation tank 1, wherein the bottom of the piston cylinder 3 is fixedly connected to a temperature control chamber 31, and the temperature control chamber 31 is provided with a temperature control part for heating the airflow in the piston cylinder 3, and a guide part for pushing the heated airflow into the interior of the cultivation tank 1 is provided in the piston cylinder 3.

[0027] Reference Figures 1-4 and Figure 8 , wherein the driving part includes a mounting bracket 4, which is fixed to the top of the cultivation tank 1, and a driving motor 41 is fixedly connected to the top of the mounting bracket 4, and a reducer 42 is fixedly connected to the bottom of the mounting bracket 4, and the output shaft of the driving motor 41 is connected to the input end of the reducer 42, and the top of the stirring shaft 2 is connected to the output end of the reducer 42; the temperature control part includes a medium pipe 5, which is fixed in the temperature control chamber 31, and the medium pipe 5 is arranged in a serpentine shape, and a temperature controller 51 is fixedly connected to the side wall of the temperature control chamber 31, and the output end of the temperature controller 51 is connected to the end of the medium pipe 5, and a PLC controller 92 is fixedly connected to the top of the cultivation tank 1, and the temperature controller 51, the temperature sensor 22 and the PLC controller 92 are electrically connected.

[0028] Through the arrangement of the above structure, the driving motor 41 is turned on, and after the speed is reduced by the reducer 42, the stirring shaft 2 is driven to rotate slowly. At the same time, the stirring shaft 2 will drive multiple groups of stirring paddles 21 to rotate slowly in the cultivation tank 1. First, it helps to evenly distribute nutrients in the culture medium. Secondly, it can move the microalgae in the cultivation tank 1, help the microalgae to remain in a suspended state, and allow it to receive uniform light, thereby promoting the growth of the entire group, thereby effectively improving the cultivation effect of the microalgae. At this time, multiple groups of temperature sensors 22 will detect the temperature in the cultivation tank 1 and transmit the detection data to the PLC controller 92. At this time, the PLC controller 92 will compare the real-time collected temperature data with the temperature threshold of the microalgae growth at this stage. When the difference between the two exceeds the threshold range, the PLC controller 92 will send a heating or cooling instruction to the temperature controller 51. At this time, the temperature controller 51 will heat or cool the liquid medium in the medium tube 5. In this way, when the airflow passes through the temperature control chamber 31 and enters the cultivation tank 1, it will be heated or cooled. After entering the cultivation tank 1, the stirring effect is combined to realize the regulation of the temperature in the cultivation tank 1. Moreover, the heating or cooling effect generated by the temperature controller 51 gradually increases, and the continuously increasing heating or cooling effect is evenly distributed in the cultivation tank 1. As described above, the temperature in the cultivation tank 1 is gradually changed by the continuously flowing airflow, avoiding the situation where the microalgae experience physiological stress due to excessive temperature change, thereby ensuring the cultivation effect of the microalgae.

[0029] In addition, at different stages of microalgae growth, the cultivation temperature thresholds set are different. As described above, the temperature in the microalgae cultivation environment at different growth stages can be accurately gradient-controlled, effectively improving the microalgae cultivation effect. Among them, the temperature sensor 22, the temperature controller 51, and the PLC controller 92 all adopt existing mature technologies, so their principles are not further described.

[0030] Reference Figure 1 、 Figure 4 、 Figure 6 and Figure 7, wherein, a guide groove 6 is provided inside the stirring paddle 21, a guide channel 61 is provided inside the stirring shaft 2, the guide groove 6 is connected to the guide channel 61, an air guide ring 62 is rotatably connected to the outer wall of the stirring shaft 2, the top of the air guide ring 62 is fixedly connected to the top of the cultivation tank 1, and the top of the guide channel 61 is connected to the inner cavity of the air guide ring 62, the guide groove 6 facing the back force side of the stirring paddle 21 is connected to the inner cavity of the cultivation tank 1, the back force side of the stirring paddle 21 is fixedly connected to the ultrafiltration membrane 63, and the ultrafiltration membrane 63 covers the connection between the guide groove 6 and the cultivation tank 1; the guide part includes a piston plate 7, the piston plate 7 is slidably connected to the piston cylinder 3, and the piston A magnetic plate 71 is slidably sleeved on the outer wall of the cylinder 3, and the magnetic plate 71 and the piston plate 7 are magnetically attracted to each other. A reciprocating screw rod 72 is rotatably connected between the top of the cultivation tank 1 and the top of the temperature control chamber 31. A lifting plate 721 is threadedly sleeved on the reciprocating screw rod 72, and the side wall of the lifting plate 721 is fixedly connected to the side wall of the magnetic plate 71. The top of the reciprocating screw rod 72 is connected to the stirring shaft 2 through a pulley set 722. The bottom end of the piston cylinder 3 is connected to the temperature control chamber 31. The side wall of the temperature control chamber 31 is fixed and connected to an inflation tube 73. The other end of the inflation tube 73 is connected to the inner cavity of the air guide ring 62, and a one-way valve is provided in the inflation tube 73.

[0031] It should be noted that the one-way valve in the inflation tube 73 can only allow the gas in the piston cylinder 3 to be charged into the liquid level of the cultivation tank 1.

[0032] Through the arrangement of the above structure, when the stirring shaft 2 rotates, the transmission effect of the pulley group 722 is utilized. When the stirring shaft 2 rotates, the reciprocating screw rod 72 will be driven to rotate. At this time, the lifting plate 721 will slide along the reciprocating screw rod 72 with the magnetic plate 71, and the piston plate 7 in the piston cylinder 3 will also move with the magnetic plate 71 by utilizing the mutual attraction between the magnetic plate 71 and the piston plate 7. When the piston plate 7 moves toward the bottom of the piston cylinder 3, the gas in the piston cylinder 3 will be compressed, and the one-way valve in the inflation tube 73 will be opened, so that the airflow in the piston cylinder 3 enters the guide groove 6 along the inflation tube 73, the air guide ring 62, and the guide channel 61, and finally passes through the ultrafiltration membrane 63 into the cultivation tank 1. With the above stirring and the floating effect of the airflow, the microalgae are evenly contacted with the carbon dioxide in the air, achieving uniform respiration, and further improving the cultivation effect of microalgae.

[0033] Reference Figure 1 、 Figure 3 and Figure 4 Among them, the lower part of the side wall of the piston cylinder 3 is fixed and connected with an exhaust pipe 74, the other end of the exhaust pipe 74 is connected to the top of the inner cavity of the culture tank 1, and an air guide pipe 75 is fixed and connected on the top side wall of the culture tank 1, and a one-way valve is provided in the exhaust pipe 74.

[0034] It should be noted that the one-way valve in the exhaust pipe 74 can only allow the gas in the top cavity of the cultivation tank 1 to enter the piston cylinder 3.

[0035] Through the arrangement of the above structure, when the piston plate 7 moves toward the top of the piston cylinder 3, a suction effect will be generated at the bottom of the piston cylinder 3, thereby opening the one-way valve in the exhaust pipe 74, so that the airflow in the top cavity of the cultivation tank 1 is sucked into the piston cylinder 3, and the airflow inside and outside the cultivation tank 1 will also be exchanged through the air guide pipe 75, thereby ensuring that the air subsequently filled into the cultivation tank 1 contains sufficient carbon dioxide, thereby meeting the growth needs of microalgae and improving the cultivation speed of microalgae.

[0036] Reference Figure 4 、 Figure 5 , wherein, a sealing ring 8 is fixedly connected to the upper part of the side wall of the cultivation tank 1, and the sealing ring 8 is connected to the cultivation tank 1 on the side facing the same, and a rotating ring 81 is slidably connected inside the sealing ring 8, and two groups of scraping rods 82 are symmetrically fixed on the side of the rotating ring 81 facing the center of the cultivation tank 1, and multiple groups of windward blades 83 are fixed at equal intervals on the side of the rotating ring 81 located inside the sealing ring 8, and a thrust tube 84 is fixed to the upper part of the side wall of the piston cylinder 3 and is connected to it, and the other end of the thrust tube 84 is connected to the inner cavity of the sealing ring 8, and the communicating port of the thrust tube 84 is inclined toward the windward blade 83.

[0037] Through the arrangement of the above structure, when the piston plate 7 moves toward the top of the piston cylinder 3, the airflow in the upper cavity of the piston cylinder 3 will be compressed at the same time, and the airflow will enter the sealing ring 8 along the thrust tube 84 and continue to blow toward the windward blade 83, thereby pushing the rotating ring 81 to rotate in the sealing ring 8. At this time, the rotating ring 81 will drive the scraping rods 82 on both sides to rotate slowly along the inner wall of the cultivation tank 1, thereby cleaning the inner wall of the cultivation tank 1, effectively preventing microalgae from adhering to the inner wall of the cultivation tank 1, improving the cleanliness of the inner wall of the cultivation tank 1, and reducing the situation where microalgae block the transparent inner wall of the cultivation tank 1, so that management personnel can observe the microalgae being cultivated.

[0038] Reference Figures 1-8In the present invention, when in use, the microalgae are first placed in the cultivation tank 1 for cultivation. During the cultivation process, the driving motor 41 is turned on, and after the speed is reduced by the reducer 42, the stirring shaft 2 is driven to rotate slowly. At the same time, the stirring shaft 2 will drive multiple groups of stirring paddles 21 to rotate slowly in the cultivation tank 1. First, it helps to evenly distribute nutrients in the culture medium. Secondly, it can move the microalgae in the cultivation tank 1, help the microalgae to maintain a suspended state, and allow it to receive uniform light, thereby promoting the growth of the entire group, thereby effectively improving the cultivation effect of the microalgae. At the same time, by utilizing the transmission effect of the pulley set 722, when the stirring shaft 2 rotates, the reciprocating screw rod 72 will be driven to rotate. At this time, the lifting plate 721 will slide along the reciprocating screw rod 72 with the magnetic plate 71, and by utilizing the mutual attraction between the magnetic plate 71 and the piston plate 7, the piston plate 7 in the piston cylinder 3 will also move with the magnetic plate 71. When the piston plate 7 moves to the bottom of the piston cylinder 3, the gas in the piston cylinder 3 will be compressed and the one-way valve in the inflation pipe 73 will be opened, so that the air flow in the piston cylinder 3 will enter the guide groove 6 along the inflation pipe 73, the air guide ring 62, and the guide channel 61, and finally pass through the ultrafiltration membrane 63 to enter the cultivation tank 1 In combination with the above-mentioned stirring and the upward effect of the airflow, the microalgae are evenly in contact with the carbon dioxide in the air, achieving uniform respiration, and further improving the cultivation effect of the microalgae; and when the piston plate 7 moves toward the top of the piston cylinder 3, a suction effect is generated at the bottom of the piston cylinder 3, thereby opening the one-way valve in the exhaust pipe 74, so that the air in the top cavity of the cultivation tank 1 is sucked into the piston cylinder 3, and the air inside and outside the cultivation tank 1 are also exchanged through the air guide pipe 75, thereby ensuring that the air subsequently filled into the cultivation tank 1 contains sufficient carbon dioxide, thereby meeting the growth needs of the microalgae and improving the cultivation speed of the microalgae.

[0039] And when the piston plate 7 moves toward the top of the piston cylinder 3, it will compress the airflow in the upper cavity of the piston cylinder 3 at the same time, and make the airflow enter the sealing ring 8 along the thrust tube 84, and continue to blow toward the windward blade 83, thereby pushing the rotating ring 81 to rotate in the sealing ring 8. At this time, the rotating ring 81 will drive the scraping rods 82 on both sides to rotate slowly along the inner wall of the cultivation tank 1, thereby cleaning the inner wall of the cultivation tank 1, effectively preventing microalgae from adhering to the inner wall of the cultivation tank 1, improving the cleanliness of the inner wall of the cultivation tank 1, and reducing the situation where microalgae block the transparent inner wall of the cultivation tank 1, so that management personnel can observe the microalgae being cultivated.

[0040] As the stirring paddles 21 rotate, the multiple sets of temperature sensors 22 detect the temperature inside the cultivation tank 1 and transmit the detection data to the PLC controller 92. At this time, the PLC controller 92 compares the real-time temperature data with the temperature threshold for microalgae growth at this stage. When the difference between the two exceeds the threshold range, the PLC controller 92 sends a temperature increase or decrease command to the temperature controller 51. At this time, the temperature controller 51 heats or cools the liquid medium in the medium tube 5. When the airflow passes through the temperature control chamber 31 and enters the cultivation tank 1, it is heated or cooled. After entering the cultivation tank 1, the stirring effect is combined to achieve temperature control in the cultivation tank 1. The heating or cooling effect generated by the temperature controller 51 gradually increases, and the continuously increasing heating or cooling effect is evenly distributed throughout the cultivation tank 1. As described above, the continuous flow of air achieves a gradual change in the temperature inside the cultivation tank 1, avoiding the physiological stress of the microalgae caused by excessive temperature changes, thereby ensuring the cultivation effect of the microalgae.

[0041] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. A precise temperature gradient control method, characterized in that: The steps include: Step 1: Obtain the temperature inside the microalgae cultivation device; Step 2: Calculate the difference between the temperature obtained in step 1 and the temperature required for the actual growth stage of the microalgae; Step 3: Determine whether the temperature in the cultivation device meets the requirements of the microalgae growth stage at this time through the difference; Step 4: When the temperature difference exceeds the set cultivation threshold, the temperature in the cultivation device is adjusted in real time.

2. A precise temperature gradient control device for implementing the precise temperature gradient control method described in claim 1, characterized in that: The invention comprises a transparent cultivation tank (1), wherein a stirring shaft (2) is rotatably connected in the cultivation tank (1), a plurality of stirring paddles (21) are mounted on the stirring shaft (2) at equal intervals, at least one temperature sensor (22) is arranged on the stirring paddles (21) on the same plane, and a driving unit for driving the stirring shaft (2) to rotate is arranged on the cultivation tank (1), and further comprises: A piston cylinder (3), wherein the piston cylinder (3) is fixed on the cultivation tank (1), The bottom of the piston cylinder (3) is fixedly connected to a temperature control chamber (31), and the temperature control chamber (31) is provided with a temperature control portion for heating the airflow in the piston cylinder (3), and a guide portion for pushing the heated airflow into the interior of the cultivation tank (1) is provided in the piston cylinder (3).

3. A precise temperature gradient control device according to claim 2, characterized in that: The driving unit comprises a mounting frame (4), the mounting frame (4) being fixed to the top of the cultivation tank (1), the top of the mounting frame (4) being fixedly connected to a driving motor (41), the bottom of the mounting frame (4) being fixedly connected to a reducer (42), the output shaft of the driving motor (41) being connected to the input end of the reducer (42), and the top end of the stirring shaft (2) being connected to the output end of the reducer (42).

4. The precise temperature gradient control device according to claim 2, characterized in that: The temperature control unit comprises a medium tube (5), the medium tube (5) is fixed in the temperature control chamber (31), and the medium tube (5) is arranged in a serpentine shape. A temperature controller (51) is fixedly connected to the side wall of the temperature control chamber (31), and the output end of the temperature controller (51) is connected to the end of the medium tube (5).

5. The precise temperature gradient control device according to claim 2, characterized in that: A guide groove (6) is provided inside the stirring paddle (21), a guide channel (61) is provided inside the stirring shaft (2), the guide groove (6) is connected to the guide channel (61), an air guide ring (62) is rotatably connected to the outer wall of the stirring shaft (2), the top of the air guide ring (62) is fixedly connected to the top of the cultivation tank (1), and the top of the guide channel (61) is connected to the inner cavity of the air guide ring (62), the guide groove (6) facing the back force surface of the stirring paddle (21) is connected to the inner cavity of the cultivation tank (1), the back force surface of the stirring paddle (21) is fixedly connected to an ultrafiltration membrane (63), and the ultrafiltration membrane (63) covers the connection between the guide groove (6) and the cultivation tank (1).

6. The precise temperature gradient control device according to claim 5, characterized in that: The guide portion includes a piston plate (7), the piston plate (7) is slidably connected in the piston cylinder (3), a magnetic plate (71) is slidably sleeved on the outer wall of the piston cylinder (3), the magnetic plate (71) and the piston plate (7) are magnetically attracted to each other, a reciprocating screw (72) is rotatably connected between the top of the culture tank (1) and the top of the temperature control chamber (31), a lifting plate (721) is threadedly sleeved on the reciprocating screw (72), and the lifting plate (72 1) is fixedly connected to the side wall of the magnetic plate (71), the top end of the reciprocating screw (72) is connected to the stirring shaft (2) through a pulley group (722), the bottom end of the piston cylinder (3) is connected to the temperature control chamber (31), the side wall of the temperature control chamber (31) is fixed and connected to an inflation tube (73), the other end of the inflation tube (73) is connected to the inner cavity of the air guide ring (62), and a one-way valve is provided in the inflation tube (73).

7. The precise temperature gradient control device according to claim 6, characterized in that: The lower part of the side wall of the piston cylinder (3) is fixed and connected to an exhaust pipe (74), the other end of the exhaust pipe (74) is connected to the top of the inner cavity of the cultivation tank (1), and an air guide pipe (75) is fixed and connected to the top side wall of the cultivation tank (1), and a one-way valve is provided in the exhaust pipe (74).

8. The precise temperature gradient control device according to claim 6, characterized in that: A sealing ring (8) is fixedly connected to the upper part of the side wall of the cultivation tank (1), and the sealing ring (8) is connected to the cultivation tank (1) on the side facing the sealing ring (8), and a rotating ring (81) is slidably connected inside the sealing ring (8), and two groups of scraping rods (82) are symmetrically fixed on the side of the rotating ring (81) facing the center of the cultivation tank (1), and a plurality of groups of windward blades (83) are fixed at equal intervals on the side of the rotating ring (81) located inside the sealing ring (8), and a thrust tube (84) is fixed to the upper part of the side wall of the piston cylinder (3) and is connected thereto, and the other end of the thrust tube (84) is connected to the inner cavity of the sealing ring (8), and the connecting port of the thrust tube (84) is inclined toward the windward blades (83).

9. The precise temperature gradient control device according to claim 2, characterized in that: Both sides of the top of the cultivation tank (1) are fixed and connected with an addition pipe (9), the top of the addition pipe (9) is fixedly connected to a sealing cover, the bottom of the cultivation tank (1) is fixedly connected to a discharge pipe (91), and a solenoid valve is provided in the discharge pipe (91).

10. The precise temperature gradient control device according to claim 4, characterized in that: A PLC controller (92) is fixedly connected to the top of the cultivation tank (1), and the temperature controller (51), the temperature sensor (22) and the PLC controller (92) are electrically connected.

Images