High-efficiency microbial compound fertilizer and preparation method thereof

Abstract

The application relates to the technical field of microbial compound fertilizers, and discloses a high-efficiency microbial compound fertilizer and a preparation method thereof, which comprises the following raw materials in parts by mass: urea 20-25 parts, monoammonium phosphate 10-15 parts, diammonium phosphate 5-8 parts, potassium dihydrogen sulfate 10-15 parts, potassium sulfate 5-10 parts, calcium-magnesium phosphate fertilizer 5-7 parts, a microbial component 10-15 parts and a composite filler 2-5 parts. The composite filler enhances the adsorption and slow-release effect on the nutrient components in the fertilizer, reduces the nutrient loss of the fertilizer, and can be used as a carrier of the microbial strains, so that the microbial strains are prevented from being affected by soil temperature, humidity and pH value, the activity of the microbial strains is inhibited, and the effect of the compound fertilizer is affected.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microbial compound fertilizer production, in particular to a high-efficiency microbial compound fertilizer and a preparation method thereof. BACKGROUND

[0002] Microbial fertilizer, also known as biological fertilizer, inoculant or bacterial fertilizer, etc., refers to a kind of fertilizer product taking the life activity of microorganisms as the core to make crops obtain specific fertilizer effect. Microbial compound fertilizer belongs to the category of compound fertilizer, including the combination of multiple microorganisms, the combination of beneficial microorganisms and different additives, etc. The additives include the combination of various additives such as organic matter (livestock and poultry manure, leather charcoal, lignite, etc.), inorganic matter (fertilizer, trace elements), etc.

[0003] The microbial fertilizer has the problems of large loss of nutrients such as nitrogen, phosphorus and potassium, low microbial activity of the fertilizer, low utilization rate of the microbial fertilizer, and the microorganism strains in the microbial fertilizer are easily affected by soil temperature, humidity and pH value, so that the activity of the microorganism strains is inhibited, thereby affecting the effect of the compound fertilizer. The addition of sepiolite in the microbial fertilizer can effectively absorb beneficial components such as nitrogen, phosphorus and potassium, and reduce the loss of the fertilizer. However, sepiolite has the defects of small surface acidity and small channel, which affects the quality of the microbial fertilizer. SUMMARY

[0004] The present application provides a high-efficiency microbial compound fertilizer and a preparation method thereof, which solves the problems of easy loss of beneficial components in the microbial compound fertilizer and inhibition of the activity of microorganism strains.

[0005] The technical scheme of the present application is as follows:

[0006] A high-efficiency microbial compound fertilizer, comprising the following raw materials in parts by mass: urea 20-25 parts, monoammonium phosphate 10-15 parts, diammonium phosphate 5-8 parts, potassium dihydrogen sulfate 10-15 parts, potassium sulfate 5-10 parts, calcium-magnesium phosphate 5-7 parts, microbial component 10-15 parts, and composite filler 2-5 parts.

[0007] A preparation method of a high-efficiency microbial compound fertilizer, comprising the following preparation steps:

[0008] S1. Mixing urea, monoammonium phosphate, diammonium phosphate, potassium dihydrogen sulfate, potassium sulfate and calcium-magnesium phosphate, and stirring at 60-80 r / min for 5-10 min to obtain a mixture;

[0009] S2. Mixing the microbial component, the composite filler and the mixture, stirring at 60-80 r / min for 5-10 min, drying at 65-75 DEG C for 20-30 min after granulation, screening, packaging, and obtaining the microbial compound fertilizer.

[0010] The composite filler is obtained by mixing and reacting modified sepiolite, hydroxypropyl methyl cellulose, ferric chloride and tea polyphenol, and then freeze-drying.

[0011] The modified sepiolite is obtained by mixing and reacting sepiolite treated by hydrothermal treatment, magnesium chloride hexahydrate and sodium hydroxide after in-situ synthesis of porous biochar on the surface of the sepiolite treated by hydrothermal treatment.

[0012] The microbial component is obtained by mixing and drying the fermentation broth formed by fermentation of the composite strains and the organic matter powder.

[0013] Further, the composite filler is specifically prepared by the following steps:

[0014] A1. mixing sepiolite and deionized water, stirring uniformly, maintaining a pressure of 1-1.5 MPa, and performing hydrothermal treatment at 150-200℃ for 3-7h, cooling to room temperature, filtering, washing, and drying to obtain pretreated sepiolite;

[0015] A2. adding pretreated sepiolite, corn straw and tannic acid to deionized water, stirring and reacting, filtering, washing, drying, adding potassium hydroxide solution, passing nitrogen, carbonizing at 750-850℃ for 3-5h, cooling to room temperature, filtering, washing, and drying to obtain sepiolite loaded with porous carbon;

[0016] A3. mixing sepiolite loaded with porous carbon and magnesium chloride hexahydrate solution, stirring uniformly, adding sodium hydroxide, stirring, filtering, washing, drying, calcining at 450-550℃ for 4-6h, and cooling to room temperature to obtain modified sepiolite;

[0017] A4. adding hydroxypropyl methyl cellulose and modified sepiolite to deionized water, stirring uniformly, adding tea polyphenol and ferric chloride, stirring to a gel, washing the gel, and freeze-drying to obtain the composite filler.

[0018] Further, in the above A1 reaction process, the sepiolite is subjected to hydrothermal treatment at 150-200℃, which can remove adsorbed water in the sepiolite, decompose organic impurities, expand the pore size of the sepiolite and increase the porosity, and obtain pretreated sepiolite.

[0019] Further, in the above A2 reaction process, tannic acid acts as a binder, allowing corn straw to adhere to the surface of the pretreated sepiolite through tannic acid, and forming a dense carbon layer after high-temperature carbonization. Potassium hydroxide solution acts as an activator, which can form pores on the surface of the dense carbon layer, and thus synthesize porous biochar on the surface of the pretreated sepiolite. The porous biochar has a pore size of 100-200nm and a particle size of 3-5μm, and the sepiolite loaded with porous carbon is obtained.

[0020] Further, in the A3 reaction process, the sepiolite loaded with porous carbon has high adsorption performance and large pore size, and can adsorb magnesium chloride hexahydrate into the pore channel on the surface of the modified sepiolite. The magnesium ions in the magnesium chloride hexahydrate can react with sodium hydroxide to form hydroxide in the pore channel of the modified sepiolite. After high-temperature calcination, the hydroxide is decomposed by heat to form oxide crystals. The crystals grow up to synthesize nanometer magnesium oxide with a size of 20-50 nm in the pore channel on the surface of the modified sepiolite, and the modified sepiolite is obtained.

[0021] Further, in the A4 reaction process, the ferric chloride as a crosslinking agent can combine with the oxygen-containing functional groups contained in the hydroxypropyl methyl cellulose through strong hydrogen bonds, and form a hydrogen bond network with the phenolic hydroxyl groups of tea polyphenols, and then form a three-dimensional crosslinked network structure. The modified sepiolite can also be embedded into the crosslinked network structure to form a three-dimensional network structure of the composite aerogel structure, which serves as a composite filler.

[0022] Further, in step A1, the amount ratio of the sepiolite to deionized water is (4-6) g:(70-80) mL.

[0023] Further, in step A2, the amount ratio of the pretreated sepiolite, corn straw, tannic acid, deionized water and potassium hydroxide solution is (3.5-3.8) g:(1.5-2) g:(0.1-0.3) g:(90-110) mL:(4.5-5.5) mL.

[0024] Further, in step A3, the amount ratio of the sepiolite loaded with porous carbon, magnesium chloride hexahydrate solution and sodium hydroxide is (4-5) g:(45-55) mL:(0.4-0.6) g.

[0025] Further, in step A4, the amount ratio of the hydroxypropyl methyl cellulose, modified sepiolite, deionized water, tea polyphenol and ferric chloride is (3.5-4.5) g:(1-2) g:(45-55) mL:(2-2.4) g:(0.1-0.3) g.

[0026] Further, the microbial component is prepared by the following steps:

[0027] The composite strain is inoculated into the seed culture medium at an inoculation amount of 2-5%, and cultured at 30-32°C and 180-200 rpm for 3-5 days. The fermentation broth is collected, diluted with clean water, and then mixed with the organic matter powder. After the organic matter powder fully adsorbs the fermentation broth, it is dried and dispersed into powder to obtain the microbial component.

[0028] Further, the seed culture medium formula is: glucose 15-20g / L, amino acid 8-10g / L, magnesium sulfate 0.4-0.5g / L, potassium dihydrogen phosphate 0.4-0.5g / L, and the pH value of the culture medium is 7.0-7.2.

[0029] Further, the organic matter powder is selected from any one of sawdust, bran and straw.

[0030] Further, the compound bacteria are obtained by mixing bacillus subtilis, bacillus megaterium and azotobacter chroococcum according to the viable bacterial number ratio of (5-10):(5-10):(1-3).

[0031] The present application has the following beneficial effects:

[0032] (1) In the technical scheme of the present application, the sepiolite is subjected to hydrothermal treatment to remove the adsorbed water in the sepiolite and decompose the organic impurities, thereby expanding the pore diameter of the sepiolite, improving the porosity, enhancing the adsorption performance of the sepiolite, and further enhancing the adsorption of the nutrient substances in the fertilizer, and reducing the nutrient loss of the fertilizer.

[0033] (2) In the technical scheme of the present application, the porous biochar is synthesized on the surface of the pretreated sepiolite. On the one hand, the synthesized porous biochar contains abundant pore structures, and the combination of the pretreated sepiolite improves the adsorption of the nutrient substances in the fertilizer, further reduces the nutrient loss of the fertilizer, and the layered silicate structure of the pretreated sepiolite has high mechanical strength, avoiding the structural brittleness of the porous biochar due to high porosity. On the other hand, the porous structure of the biochar has a physical adsorption effect on organic pollutants (such as heavy metals), and the silicon hydroxyl groups on the surface of the pretreated sepiolite can enhance the adsorption of heavy metals through coordination complexation, reduce the pollution of heavy metals to the soil, and improve the soil fertility. In addition, the pretreated sepiolite and the porous biochar can adsorb nutrient ions such as potassium, calcium and magnesium in the fertilizer and slowly release them, thereby improving the soil fertility.

[0034] (3) In the technical scheme of the present application, nano magnesium oxide is synthesized in the pore channel of the modified sepiolite. On the one hand, the synthesized nano magnesium oxide provides a large amount of magnesium elements, which are one of the medium nutrient elements necessary for the normal growth and development of plants, and can promote the growth of crops. At the same time, magnesium elements can alleviate salt stress and improve fertility. On the other hand, nano magnesium oxide can refine the pore diameter of the porous biochar in the modified sepiolite, improve the adsorption performance, and further enhance the adsorption and slow release of the nutrient components in the fertilizer. Moreover, as the skeleton structure of the porous biochar, nano magnesium oxide avoids the stress of the porous biochar and the fertilizer during the granulation and screening process, thereby preventing the collapse of the pore channel of the porous biochar and affecting the adsorption of the nutrient substances.

[0035] (4) In the technical scheme of the present application, the modified sepiolite, hydroxypropyl methyl cellulose, ferric chloride and tea polyphenol are mixed and reacted to form a three-dimensional network structure composite aerogel, the hydroxypropyl methyl cellulose acts as a skeleton and contains a large number of functional groups, thereby enhancing the water retention performance of the composite filler, and the composite aerogel has a porous structure, thereby increasing the adsorption of the fertilizer, in addition, the composite aerogel can be uniformly dispersed in the microbial compound fertilizer, thereby improving the fertility of the microbial compound fertilizer.

[0036] (5) In the technical scheme of the present application, the diluted microbial fermentation liquor is added to the organic matter powder, so that the organic matter fully adsorbs the microbial fermentation liquor, and is dried at low temperature and scattered into powder, thereby obtaining a microbial component, so that the microbial fermentation liquor is prevented from directly penetrating into part of the compound fertilizer, thereby affecting the mixing with other parts of the compound fertilizer, the uniformity of the microorganism is improved, and the composite filler can also act as a carrier of the microbial strain, so that the microbial strain is prevented from being affected by the soil temperature, humidity, and pH value, thereby preventing the activity of the microbial strain from being inhibited, and thereby affecting the effect of the compound fertilizer. DETAILED DESCRIPTION

[0037] The technical solutions in the embodiments of the present application will be clearly and completely described below in combination with the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by a person of ordinary skill in the art without creative labor fall within the protection scope of the present application.

[0038] The raw materials used in the embodiments of the present application are shown as follows, and all the reagents used are analytical grade.

[0039] Among them, the Bacillus subtilis is numbered ACCC 60429, the Bacillus megaterium is numbered CICC 20665, and the Azotobacter chroococcum is numbered CICC 22661, which are all purchased from the China Industrial Microbial Culture Collection Center.

[0040] The sepiolite particle size is 45 μm, which is purchased from the Lingshou County Yuanda Mica Factory.

[0041] The organic matter powder is selected from sawdust.

[0042] Embodiment 1

[0043] A high-efficiency microbial compound fertilizer comprises the following raw materials in parts by mass: urea 20 parts, monoammonium phosphate 10 parts, diammonium phosphate 5 parts, potassium hydrogen sulfate 10 parts, potassium sulfate 5 parts, calcium magnesium phosphate 5 parts, microbial component 10 parts, and composite filler 2 parts.

[0044] A preparation method of high-efficiency microbial compound fertilizer, comprising the following preparation steps:

[0045] S1. Urea, monoammonium phosphate, diammonium phosphate, potassium dihydrogen sulfate, potassium sulfate and calcium magnesium phosphate are mixed and stirred at 60 r / min for 5 min to obtain a mixture;

[0046] S2. The microbial component, the composite filler and the mixture are mixed and stirred at 60 r / min for 5 min, and after granulation, drying is performed at 65 ℃ for 20 min, screening is performed, and packaging is performed to obtain the microbial compound fertilizer.

[0047] The composite filler is specifically prepared by the following steps:

[0048] A1. 4 g of sepiolite and 70 mL of deionized water are mixed and uniformly stirred, a pressure of 1 MPa is maintained, hydrothermal treatment is performed at 150 ℃ for 3 h, cooling to room temperature is performed, filtration is performed, deionized water washing is performed for 3 times, and drying is performed in an oven at 80 ℃ for 10 min to obtain pretreated sepiolite;

[0049] A2. 3.5 g of the pretreated sepiolite, 1.5 g of corn straw and 0.1 g of tannic acid are added to 90 mL of deionized water, stirring reaction is performed at 75 ℃ for 30 min, filtration is performed, deionized water washing is performed for 3 times, drying is performed in an oven at 70 ℃ for 10 min, 4.5 mL of a 30% potassium hydroxide solution is added, nitrogen is introduced, carbonization is performed at 750 ℃ for 3 h, cooling to room temperature is performed, filtration is performed, deionized water washing is performed for 3 times, and drying is performed in an oven at 70 ℃ for 10 min to obtain sepiolite loaded with porous carbon;

[0050] A3. 4 g of the sepiolite loaded with porous carbon and 45 mL of a 0.2 mol / L magnesium chloride hexahydrate solution are mixed and uniformly stirred, 0.4 g of sodium hydroxide is added, stirring is performed at 30 ℃ for 30 min, filtration is performed, deionized water washing is performed for 3 times, drying is performed in an oven at 80 ℃ for 2 h, calcination is performed in a muffle furnace at 450 ℃ for 4 h, and cooling to room temperature is performed to obtain modified sepiolite;

[0051] A4. 3.5 g of hydroxypropyl methyl cellulose and 1 g of the modified sepiolite are added to 45 mL of deionized water, uniformly stirred, 2 g of tea polyphenol and 0.1 g of ferric chloride are added, stirring is performed at 350 r / min until a gel is formed, the gel is washed with deionized water for 3 times, freeze drying is performed at -20 ℃ for 24 h to obtain the composite filler.

[0052] The microbial component is specifically prepared by the following steps:

[0053] The complex bacteria is inoculated into a seed culture medium at an inoculation amount of 2%, and is cultured at 30 DEG C and 180 rpm for 3 days, and the fermentation liquor is collected, diluted 10 times with clean water, mixed with the sawdust, dried at 30 DEG C after the sawdust fully absorbs the fermentation liquor for 38 min, and is broken into powder to obtain a microbial component, wherein the content of the sawdust is 25%.

[0054] The seed culture medium formula is: glucose 15 g / L, amino acid 8 g / L, magnesium sulfate 0.4 g / L, potassium dihydrogen phosphate 0.4 g / L, and the pH value of the culture medium is 7.0.

[0055] The complex bacteria is obtained by mixing Bacillus subtilis, Bacillus megaterium and Azotobacter chroococcum according to the live bacteria ratio of 5:5:1.

[0056] Example 2

[0057] A high-efficiency microbial compound fertilizer, comprising the following raw materials in parts by mass: urea 23 parts, monoammonium phosphate 13 parts, diammonium phosphate 7 parts, potassium dihydrogen sulfate 13 parts, potassium sulfate 8 parts, calcium magnesium phosphate 6 parts, microbial component 13 parts, and composite filler 4 parts.

[0058] A preparation method of a high-efficiency microbial compound fertilizer, comprising the following preparation steps:

[0059] S1. Mixing urea, monoammonium phosphate, diammonium phosphate, potassium dihydrogen sulfate, potassium sulfate, and calcium magnesium phosphate, and stirring at 70 r / min for 8 min to obtain a mixture;

[0060] S2. Mixing the microbial component, the composite filler, and the mixture, and stirring at 70 r / min for 8 min, granulating, drying at 70 DEG C for 25 min, screening, and packaging to obtain the microbial compound fertilizer.

[0061] The composite filler is specifically prepared by the following steps:

[0062] A1. Mixing 5 g of sepiolite and 75 mL of deionized water, stirring uniformly, maintaining a pressure of 1.3 MPa, and performing hydrothermal treatment at 180 DEG C for 5 h, cooling to room temperature, filtering, washing with deionized water for 3 times, and drying in an 80 DEG C oven for 10 min to obtain pretreated sepiolite;

[0063] A2. 3.7 g of pre-treated sepiolite, 1.8 g of corn straw and 0.2 g of tannic acid were added to 100 mL of deionized water, stirred at 75℃ for 30 min, filtered, washed with deionized water for 3 times, dried in an oven at 70℃ for 10 min, placed in a tube furnace, 5 mL of 30% potassium hydroxide solution was added, nitrogen was introduced, carbonized at 800℃ for 4 h, cooled to room temperature, filtered, washed with deionized water for 3 times, dried in an oven at 70℃ for 10 min, to obtain sepiolite loaded with porous carbon;

[0064] A3. 4.5 g of sepiolite loaded with porous carbon and 50 mL of 0.2 mol / L magnesium chloride hexahydrate solution were mixed, stirred uniformly, 0.5 g of sodium hydroxide was added, stirred at 30℃ for 30 min, filtered, washed with deionized water for 3 times, dried in an oven at 80℃ for 2 h, calcined in a muffle furnace at 500℃ for 5 h, cooled to room temperature, to obtain modified sepiolite;

[0065] A4. 4 g of hydroxypropyl methyl cellulose and 1.5 g of modified sepiolite were added to 50 mL of deionized water, stirred uniformly, 2.2 g of tea polyphenol and 0.2 g of ferric chloride were added, stirred at 350 r / min until gelling, the gel was washed with deionized water for 3 times, freeze-dried at -20℃ for 24 h, to obtain a composite filler.

[0066] The microbial component was prepared by the following steps:

[0067] The composite strain was inoculated into a seed culture medium at an inoculation amount of 3%, cultured at 31℃, 190 rpm for 4 days, the fermentation broth was collected, diluted 10 times with clean water, then mixed with sawdust, after the sawdust fully absorbed the fermentation broth, dried at 30℃ for 38 min, broken into powder, to obtain a microbial component, wherein the content of organic matter powder was 25%;

[0068] The formula of the seed culture medium was: glucose 18 g / L, amino acid 9 g / L, magnesium sulfate 0.45 g / L, potassium dihydrogen phosphate 0.45 g / L, and the pH value of the culture medium was 7.1.

[0069] The composite strain was obtained by mixing Bacillus subtilis, Bacillus megaterium and Azotobacter chroococcum according to the live bacteria ratio of 8:8:2.

[0070] Example 3

[0071] A kind of high-efficiency microbial compound fertilizer, including the following mass parts of raw materials: urea 25 parts, monoammonium phosphate 15 parts, diammonium phosphate 8 parts, potassium dihydrogen sulfate 15 parts, potassium sulfate 10 parts, calcium magnesium phosphate 7 parts, microbial component 15 parts, composite filler 5 parts;

[0072] A preparation method of high-efficiency microbial compound fertilizer, comprising the following preparation steps:

[0073] S1. Urea, monoammonium phosphate, diammonium phosphate, potassium dihydrogen sulfate, potassium sulfate, calcium magnesium phosphate fertilizer are mixed, stirred at 80 r / min for 10 min to obtain a mixture;

[0074] S2. The microbial component, the composite filler and the mixture are mixed, stirred at 80 r / min for 10 min, dried at 75 DEG C for 30 min after granulation, screened, packaged to obtain the microbial compound fertilizer.

[0075] The composite filler is specifically prepared by the following steps:

[0076] A1. 6g sepiolite and 80mL deionized water are mixed, stirred uniformly, maintained at 1.5MPa pressure, maintained at 1.5MPa pressure, hydrothermally treated at 200 DEG C for 7h, cooled to room temperature, filtered, washed with deionized water for 3 times, dried in an 80 DEG C oven for 10 min to obtain pretreated sepiolite;

[0077] A2. 3.8g pretreated sepiolite, 2g corn straw and 0.3g tannic acid are added to 110mL deionized water, stirred and reacted at 75 DEG C for 30 min, filtered, washed with deionized water for 3 times, dried in a 70 DEG C oven for 10 min, placed in a tube furnace, 5.5mL 30% mass fraction potassium hydroxide solution is added, nitrogen is introduced, carbonized at 850 DEG C for 5h, cooled to room temperature, filtered, washed with deionized water for 3 times, dried in a 70 DEG C oven for 10 min to obtain sepiolite loaded with porous carbon;

[0078] A3. 5g sepiolite loaded with porous carbon and 55mL 0.2mol / L magnesium chloride hexahydrate solution are mixed, stirred uniformly, 0.6g sodium hydroxide is added, stirred at 30 DEG C for 30 min, filtered, washed with deionized water for 3 times, dried in an 80 DEG C oven for 2h, placed in a muffle furnace, calcined at 550 DEG C for 6h, cooled to room temperature to obtain modified sepiolite;

[0079] A4. 4.5g hydroxypropyl methyl cellulose and 2g modified sepiolite are added to 55mL deionized water, stirred uniformly, 2.4g tea polyphenol and 0.3g ferric chloride are added, stirred at 350r / min until gelling, the gel is washed with deionized water for 3 times, freeze-dried at-20 DEG C for 24h to obtain the composite filler.

[0080] The microbial component is specifically prepared by the following steps:

[0081] The complex bacteria is inoculated into a seed culture medium at 5%, and cultured at 32°C and 200 rpm for 5 days, and the fermentation liquor is collected, diluted 10 times with clean water, mixed with sawdust, dried at 30°C for 38 min after the sawdust fully absorbs the fermentation liquor, and scattered into powder to obtain a microbial component, wherein the content of organic matter powder is 25%.

[0082] The seed culture medium formula is: glucose 20 g / L, amino acid 10 g / L, magnesium sulfate 0.5 g / L, potassium dihydrogen phosphate 0.5 g / L, and the pH value of the culture medium is 7.2.

[0083] The complex bacteria is obtained by mixing Bacillus subtilis, Bacillus megaterium and Azotobacter chroococcum according to the live bacteria ratio of 10:10:3.

[0084] Comparative Example 1

[0085] A kind of high-efficiency microbial compound fertilizer, including the following mass parts raw materials: urea 25 parts, monoammonium phosphate 15 parts, diammonium phosphate 8 parts, potassium hydrogen sulfate 15 parts, potassium sulfate 10 parts, calcium magnesium phosphate 7 parts, microbial component 15 parts, composite filler 5 parts.

[0086] A kind of high-efficiency microbial compound fertilizer and a preparation method thereof, including the following preparation steps:

[0087] S1.urea, monoammonium phosphate, diammonium phosphate, potassium hydrogen sulfate, potassium sulfate, calcium magnesium phosphate are mixed, stirred at 80 r / min for 10 min to obtain a mixture;

[0088] S2. The microbial component, composite filler and mixture are mixed, stirred at 80 r / min for 10 min, granulated, dried at 75°C for 30 min, screened, packaged to obtain a microbial compound fertilizer.

[0089] The composite filler is specifically prepared by the following steps:

[0090] A1.3.8g sepiolite, 2g corn straw and 0.3g tannic acid are added to 110mL deionized water, stirred and reacted at 75°C for 30min, filtered, washed with deionized water for 3 times, dried in a 70°C oven for 10min, placed in a tube furnace, 5.5mL 30% potassium hydroxide solution is added, nitrogen is introduced, carbonized at 850°C for 5h, cooled to room temperature, filtered, washed with deionized water for 3 times, dried in a 70°C oven for 10min to obtain sepiolite loaded with porous carbon;

[0091] A2. 5 g of the porous carbon loaded sepiolite and 55 mL of magnesium chloride hexahydrate solution with a concentration of 0.2 mol / L were mixed, stirred uniformly, 0.6 g of sodium hydroxide was added, stirred at 30℃ for 30 min, filtered, washed with deionized water for 3 times, dried in an oven at 80℃ for 2 h, calcined in a muffle furnace at 550℃ for 6 h, cooled to room temperature, and modified sepiolite was obtained;

[0092] A3. 4.5 g of hydroxypropyl methyl cellulose and 2 g of modified sepiolite were added to 55 mL of deionized water, stirred uniformly, 2.4 g of tea polyphenol and 0.3 g of ferric chloride were added, stirred at 350 r / min until gelling, the gel was washed with deionized water for 3 times, freeze-dried at-20℃ for 24 h, and a composite filler was obtained.

[0093] The microbial component was prepared by the following steps:

[0094] The composite strain was inoculated into the seed culture medium at an inoculation amount of 5%, cultured at 32℃, 200 rpm for 5 days, the fermentation broth was collected, diluted 10 times with clean water, then mixed with sawdust, dried at 30℃ for 38 min after the sawdust fully absorbed the fermentation broth, and dispersed into powder to obtain the microbial component, wherein the organic matter powder content was 25%.

[0095] The seed culture medium formula was: glucose 20 g / L, amino acid 10 g / L, magnesium sulfate 0.5 g / L, potassium dihydrogen phosphate 0.5 g / L, and the culture medium pH value was 7.2.

[0096] The composite strain was obtained by mixing bacillus subtilis, bacillus megaterium and azotobacter chroococcum according to the live bacteria ratio of 10:10:3.

[0097] Comparative example 2

[0098] A kind of high-efficiency microbial compound fertilizer, including the following mass parts of raw materials: urea 25 parts, monoammonium phosphate 15 parts, diammonium phosphate 8 parts, potassium hydrogen sulfate 15 parts, potassium sulfate 10 parts, calcium magnesium phosphate 7 parts, microbial component 15 parts, composite filler 5 parts;

[0099] A kind of high-efficiency microbial compound fertilizer preparation method, including the following preparation steps:

[0100] S1. urea, monoammonium phosphate, diammonium phosphate, potassium hydrogen sulfate, potassium sulfate, calcium magnesium phosphate were mixed, stirred at 80 r / min for 10 min to obtain a mixture;

[0101] S2. the microbial component, composite filler and mixture were mixed, stirred at 80 r / min for 10 min, after granulation, dried at 75℃ for 30 min, screened, packaged to obtain microbial compound fertilizer.

[0102] The composite filler is specifically prepared by the following steps:

[0103] A1. 6 g of sepiolite and 80 mL of deionized water were mixed, stirred uniformly, and subjected to hydrothermal treatment at 200 DEG C for 7 h under a pressure of 1.5 MPa, cooled to room temperature, filtered, washed with deionized water for 3 times, and dried in an oven at 80 DEG C for 10 min to obtain pretreated sepiolite;

[0104] A2. 5 g of the pretreated sepiolite and 55 mL of a magnesium chloride hexahydrate solution with a concentration of 0.2 mol / L were mixed, stirred uniformly, 0.6 g of sodium hydroxide was added, stirred at 30 DEG C for 30 min, filtered, washed with deionized water for 3 times, dried in an oven at 80 DEG C for 2 h, calcined in a muffle furnace at 550 DEG C for 6 h, and cooled to room temperature to obtain modified sepiolite;

[0105] A3. 4.5 g of hydroxypropyl methyl cellulose and 2 g of the modified sepiolite were added to 55 mL of deionized water, stirred uniformly, 2.4 g of tea polyphenol and 0.3 g of ferric chloride were added, stirred to a gel state at 350 r / min, the gel was washed with deionized water for 3 times, and freeze-dried at -20 DEG C for 24 h to obtain the composite filler.

[0106] The microbial component is specifically prepared by the following steps:

[0107] The composite strain was inoculated into a seed culture medium at an inoculation amount of 5%, and cultured at 32 DEG C and 200 rpm for 5 days, the fermentation liquor was collected, diluted 10 times with clean water, and then mixed with sawdust, the sawdust was dried at 30 DEG C after fully absorbing the fermentation liquor for 38 min, and then scattered into a powder to obtain the microbial component, wherein the content of the organic matter powder was 25%.

[0108] The seed culture medium formula was as follows: 20 g / L of glucose, 10 g / L of amino acid, 0.5 g / L of magnesium sulfate, and 0.5 g / L of potassium dihydrogen phosphate, and the pH value of the culture medium was 7.2.

[0109] The composite strain was obtained by mixing bacillus subtilis, bacillus megaterium and azotobacter chroococcum according to a live bacteria number ratio of 10:10:3.

[0110] Comparative Example 3

[0111] A kind of high-efficiency microbial compound fertilizer, including the following mass parts raw materials: urea 25 parts, monoammonium phosphate 15 parts, diammonium phosphate 8 parts, potassium dihydrogen phosphate 15 parts, potassium sulfate 10 parts, calcium magnesium phosphate 7 parts, microbial component 15 parts, composite filler 5 parts;

[0112] A kind of high-efficiency microbial compound fertilizer preparation method, including the following preparation steps:

[0113] S1. Mix urea, monoammonium phosphate, diammonium phosphate, potassium dihydrogen sulfate, potassium sulfate, calcium magnesium phosphate fertilizer, and stir at 80 r / min for 10 min to obtain a mixture;

[0114] S2. Mix the microbial component, the composite filler and the mixture, stir at 80 r / min for 10 min, dry at 75℃ for 30 min after granulation, screen, and package to obtain the microbial compound fertilizer.

[0115] The composite filler is specifically prepared by the following steps:

[0116] A1. Mix 6 g of sepiolite and 80 mL of deionized water, stir uniformly, maintain a pressure of 1.5 MPa, and perform hydrothermal treatment at 200℃ for 7 h, cool to room temperature, filter, wash with deionized water 3 times, and dry in an 80℃ oven for 10 min to obtain pretreated sepiolite;

[0117] A2. Add 3.8 g of pretreated sepiolite, 2 g of corn straw and 0.3 g of tannic acid to 110 mL of deionized water, stir at 75℃ for 30 min, filter, wash with deionized water 3 times, dry at 70℃ for 10 min, place in a tube furnace, add 5.5 mL of 30% potassium hydroxide solution, introduce nitrogen, and carbonize at 850℃ for 5 h, cool to room temperature, filter, wash with deionized water 3 times, and dry at 70℃ for 10 min to obtain sepiolite loaded with porous carbon;

[0118] A3. Add 4.5 g of hydroxypropyl methyl cellulose and 2 g of sepiolite loaded with porous carbon to 55 mL of deionized water, stir uniformly, add 2.4 g of tea polyphenols and 0.3 g of ferric chloride, stir at 350 r / min until gel is formed, wash the gel with deionized water 3 times, freeze dry at -20℃ for 24 h to obtain the composite filler.

[0119] The microbial component is specifically prepared by the following steps:

[0120] Inoculate the composite strain into a seed culture medium at an inoculation amount of 5%, culture at 32℃, 200 rpm for 5 days, collect the fermentation broth, dilute the fermentation broth 10 times with clean water, then mix with sawdust, dry at 30℃ for 38 min after the sawdust fully absorbs the fermentation broth, and disperse into powder to obtain the microbial component, wherein the content of organic matter powder is 25%;

[0121] The formula of the seed culture medium is: glucose 20 g / L, amino acid 10 g / L, magnesium sulfate 0.5 g / L, potassium dihydrogen phosphate 0.5 g / L, and the pH value of the culture medium is 7.2.

[0122] The complex bacteria is obtained by mixing bacillus subtilis, bacillus megaterium and azotobacter chroococcum according to the ratio of 10:10:3 of the number of viable bacteria.

[0123] Comparative example 4

[0124] A kind of high-efficiency microbial compound fertilizer, including the following mass parts raw materials: urea 25 parts, monoammonium phosphate 15 parts, diammonium phosphate 8 parts, potassium dihydrogen sulfate 15 parts, potassium sulfate 10 parts, calcium magnesium phosphate 7 parts, microbial component 15 parts, composite filler 5 parts;

[0125] A kind of high-efficiency microbial compound fertilizer preparation method, including the following preparation steps:

[0126] S1.mixture of urea, monoammonium phosphate, diammonium phosphate, potassium dihydrogen sulfate, potassium sulfate, calcium magnesium phosphate is mixed, stirred at 80r / min for 10min, to obtain mixed material;

[0127] S2.mixture of microbial component, composite filler and mixed material is mixed, stirred at 80r / min for 10min, after granulation, at 75 DEG C, drying 30min, screening, packaging, to obtain microbial compound fertilizer.

[0128] Composite filler is specifically prepared by the following steps:

[0129] A1.6g sepiolite and 80mL deionized water are mixed, stirred uniformly, keep 1.5MPa pressure, hydrothermal treatment is carried out at 200 DEG C for 7h, cool to room temperature, filter, deionized water washing 3 times, drying 10min in 80 DEG C oven, to obtain pretreated sepiolite;

[0130] A2.3.8g pretreated sepiolite, 2g corn straw and 0.3g tannic acid are added to 110mL deionized water, stirred at 75 DEG C for 30min, filter, deionized water washing 3 times, drying 10min in 70 DEG C oven, place in tube furnace, add 5.5mL 30% mass fraction potassium hydroxide solution, nitrogen is imported, carbonization is carried out at 850 DEG C for 5h, cool to room temperature, filter, deionized water washing 3 times, drying 10min in 70 DEG C oven, to obtain sepiolite loaded with porous carbon;

[0131] A3.5g sepiolite loaded with porous carbon and 55mL 0.2mol / L magnesium chloride hexahydrate solution are mixed, stirred uniformly, add 0.6g sodium hydroxide, stirred at 30 DEG C for 30min, filter, deionized water washing 3 times, drying 2h in 80 DEG C oven, place in muffle furnace, calcination is carried out at 550 DEG C for 6h, cool to room temperature, to obtain modified sepiolite;

[0132] A4. 6.9 g of tea polyphenol and 2 g of modified sepiolite were added to 55 mL of deionized water, stirred uniformly, 0.3 g of ferric chloride was added, stirred uniformly at 350 r / min, washed with deionized water for 3 times, and freeze-dried at -20℃ for 24 h to obtain a composite filler.

[0133] The microbial component was specifically prepared by the following steps:

[0134] The composite strain was inoculated into a seed culture medium at an inoculation amount of 5%, cultured at 32℃ and 200 rpm for 5 days, the fermentation liquor was collected, diluted 10 times with clean water, then mixed with sawdust, dried at 30℃ for 38 min after the sawdust fully absorbed the fermentation liquor, and then dispersed into powder to obtain the microbial component, wherein the content of organic matter powder was 25%.

[0135] The seed culture medium formula was: glucose 20 g / L, amino acid 10 g / L, magnesium sulfate 0.5 g / L, potassium dihydrogen phosphate 0.5 g / L, and the pH value of the culture medium was 7.2.

[0136] The composite strain was obtained by mixing Bacillus subtilis, Bacillus megaterium and Azotobacter chroococcum according to the live bacteria ratio of 10:10:3.

[0137] Comparative Example 5

[0138] A kind of high-efficiency microbial compound fertilizer, including the following mass parts raw materials: urea 25 parts, monoammonium phosphate 15 parts, diammonium phosphate 8 parts, potassium hydrogen sulfate 15 parts, potassium sulfate 10 parts, calcium magnesium phosphate 7 parts, microbial component 15 parts, composite filler 5 parts;

[0139] A kind of high-efficiency microbial compound fertilizer and a preparation method thereof, including the following preparation steps:

[0140] S1. Urea, monoammonium phosphate, diammonium phosphate, potassium hydrogen sulfate, potassium sulfate, calcium magnesium phosphate were mixed, stirred at 80 r / min for 10 min to obtain a mixture;

[0141] S2. The microbial component, composite filler and mixture were mixed, stirred at 80 r / min for 10 min, granulated, dried at 75℃ for 30 min, screened, packaged to obtain the microbial compound fertilizer.

[0142] The composite filler was specifically prepared by the following steps:

[0143] A1. 6 g of sepiolite and 80 mL of deionized water were mixed, stirred uniformly, maintained at a pressure of 1.5 MPa, hydrothermally treated at 200℃ for 7 h, cooled to room temperature, filtered, washed with deionized water for 3 times, and dried in an 80℃ oven for 10 min to obtain pretreated sepiolite;

[0144] A2. 3.8 g of pretreated sepiolite, 2 g of corn straw and 0.3 g of tannic acid were added to 110 mL of deionized water, stirred at 75°C for 30 min, filtered, washed with deionized water for 3 times, dried in an oven at 70°C for 10 min, placed in a tube furnace, 5.5 mL of 30% potassium hydroxide solution was added, nitrogen was introduced, carbonized at 850°C for 5 h, cooled to room temperature, filtered, washed with deionized water for 3 times, dried in an oven at 70°C for 10 min, to obtain sepiolite loaded with porous carbon;

[0145] A3. 5 g of sepiolite loaded with porous carbon and 55 mL of 0.2 mol / L magnesium chloride hexahydrate solution were mixed, stirred uniformly, 0.6 g of sodium hydroxide was added, stirred at 30°C for 30 min, filtered, washed with deionized water for 3 times, dried in an oven at 80°C for 2 h, placed in a muffle furnace, calcined at 550°C for 6 h, cooled to room temperature, to obtain modified sepiolite;

[0146] A4. 4.5 g of hydroxypropyl methyl cellulose and 2 g of modified sepiolite were added to 55 mL of deionized water, stirred uniformly, 2.4 g of tea polyphenol and 0.3 g of ferric chloride were added, stirred at 350 r / min until gelling, the gel was washed with deionized water for 3 times, freeze-dried at -20°C for 24 h, to obtain a composite filler.

[0147] The microbial component was prepared by the following steps:

[0148] The composite strain was inoculated into a seed culture medium at an inoculation amount of 5%, cultured at 32°C and 200 rpm for 5 days, the fermentation liquor was collected, diluted 10 times with clean water, then mixed uniformly with urea, dried at 30°C for 38 min, and dispersed into a powder to obtain the microbial component, wherein the urea content was 25%.

[0149] The seed culture medium formula was: glucose 20 g / L, amino acid 10 g / L, magnesium sulfate 0.5 g / L, potassium dihydrogen phosphate 0.5 g / L, and the pH value of the culture medium was 7.2.

[0150] The composite strain was obtained by mixing Bacillus subtilis, Bacillus megaterium and Azotobacter chroococcum at a live bacteria ratio of 10:10:3.

[0151] The effects of the microbial composite fertilizers prepared in Examples 1-3 and Comparative Examples 1-5 were detected.

[0152] 3 g of compound fertilizer is mixed with 8 kg of soil, and then loaded into a flowerpot. 20 wheat seeds are sown in each flowerpot. After the wheat sprouts, 5 wheat seedlings with the same growth are reserved in each flowerpot. After winter, the flowerpots are buried in the soil to prevent the wheat roots from being frozen. After the wheat is harvested, the whole wheat is dried and weighed to obtain the dry matter weight. The nitrogen content of the wheat plant is determined by the Nessler colorimetric method, the phosphorus content is determined by the vanadium-molybdenum yellow colorimetric method, and the potassium content is determined by the flame photometric method.

[0153] The test results are shown in Table 1 below.

[0154] Table 1

[0155]

[0156] As can be seen from the data in Table 1, the microbial compound fertilizer prepared in Examples 1-3 can increase the yield of crops and improve the soil fertility.

[0157] In Comparative Example 1, the pretreated sepiolite is replaced by the compound filler prepared by loading porous carbon on sepiolite and added to the microbial compound fertilizer. The yield of wheat decreases, and the nutrient components in wheat decrease, which proves that after the hydrothermal treatment of sepiolite, the adsorbed water in sepiolite is removed, the organic impurities are decomposed, the pore size of sepiolite is expanded, the porosity is improved, the adsorption performance of sepiolite is improved, and then the adsorption of nutrient substances in fertilizer is enhanced, and the nutrient loss of fertilizer is reduced.

[0158] In Comparative Example 2, the pretreated sepiolite is replaced by the compound filler prepared by loading porous carbon on sepiolite and added to the microbial compound fertilizer. The yield of wheat decreases, and the nutrient components in wheat decrease, which proves that the porous biochar is synthesized on the surface of the pretreated sepiolite, the adsorption of nutrient substances in fertilizer is improved, the nutrient loss of fertilizer is further reduced, the porous structure of biochar has a physical adsorption effect on organic pollutants (such as heavy metals), and the silicon hydroxyl on the surface of the pretreated sepiolite can enhance the adsorption of heavy metals through coordination complexation, reduce the pollution of heavy metals to the soil, improve the soil fertility, and further improve the soil fertility.

[0159] In Comparative Example 3, the modified sepiolite is replaced by the compound filler prepared by loading porous carbon on sepiolite and added to the microbial compound fertilizer. The yield of wheat decreases, and the nutrient components in wheat decrease, which proves that the nano magnesium oxide is synthesized in the pore channel on the surface of the modified sepiolite, which can promote the growth of crops, and the magnesium element can alleviate the salt stress and improve the fertility. Moreover, the nano magnesium oxide serves as the skeleton structure of the porous biochar, which avoids the porous biochar from being subjected to stress during the granulation and screening process of the fertilizer, so as to prevent the pore channel of the porous biochar from collapsing and affecting the adsorption of nutrient substances.

[0160] The wheat yield and the nutrient components in the wheat decrease in the comparative example 4 in which the hydroxypropyl methyl cellulose is replaced by the composite filler prepared from tea polyphenol and added to the microbial compound fertilizer, which proves that the modified sepiolite, the hydroxypropyl methyl cellulose, the ferric chloride and the tea polyphenol mixed reaction can form the composite aerogel with the three-dimensional network structure, the hydroxypropyl methyl cellulose serves as the skeleton, contains a large number of functional groups, enhances the water retention performance of the composite filler, and has the porous structure, increases the adsorption of the fertilizer, in addition, the composite aerogel can be uniformly dispersed in the microbial compound fertilizer, can serve as the carrier of the microbial strains, avoids the microbial strains from being affected by the soil temperature, humidity, pH value, and causes the activity of the microbial strains to be inhibited, thereby affecting the effect of the compound fertilizer, and improves the fertility of the microbial compound fertilizer.

[0161] The wheat yield and the nutrient components in the wheat decrease in the comparative example 5 in which the sawdust is replaced by the microbial component prepared from urea and added to the microbial compound fertilizer, which proves that the diluted microbial fermentation liquor can be added to the organic matter powder, can avoid the microbial fermentation liquor from directly penetrating in part of the compound fertilizer, affects the mixing with other parts of the compound fertilizer, and improves the uniformity of the microorganisms.

[0162] In the description of the specification, the description of the terms "one embodiment", "example", "specific example" and the like means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are contained in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily mean the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0163] The above is only an example and description of the present application, and those skilled in the art can make various modifications or supplements to the described specific embodiments or adopt similar ways to replace, as long as the modifications or supplements do not deviate from the application or exceed the scope defined by the present claims, and should belong to the protection scope of the present application.

Claims

1. A high-efficiency microbial compound fertilizer, characterized in that, The compound filler is prepared by the following steps: A1. The sepiolite and deionized water are mixed and stirred uniformly, and then the mixture is subjected to hydrothermal treatment at 150-200 DEG C for 3-7 hours under a pressure of 1-1.5 MPa, and then cooled to room temperature, filtered, washed and dried to obtain pretreated sepiolite; A2. The pretreated sepiolite, corn straw and tannic acid are added to deionized water, and then subjected to stirring reaction, filtration, washing and drying, and then a potassium hydroxide solution is added, inert gas is introduced, and carbonization is carried out at 750-850 DEG C for 3-5 hours, and then the mixture is cooled to room temperature, filtered, washed and dried to obtain sepiolite loaded with porous carbon; A3. The sepiolite loaded with porous carbon and a magnesium chloride hexahydrate solution are mixed and stirred uniformly, and then sodium hydroxide is added, and then the mixture is subjected to stirring, filtration, washing and drying, and then calcination is carried out at 450-550 DEG C for 4-6 hours, and then the mixture is cooled to room temperature to obtain modified sepiolite; A4. The hydroxypropyl methyl cellulose and modified sepiolite are added to deionized water, and then the mixture is stirred uniformly, and then tea polyphenol and ferric chloride are added, and then the mixture is stirred to a gel state, and then the gel is washed and freeze-dried to obtain the compound filler; The organic matter powder is selected from any one of sawdust, bran and straw; The compound bacterial strain is obtained by mixing Bacillus subtilis, Bacillus megaterium and Azotobacter chroococcum according to a ratio of (5-10):(5-10):(1-3) of the number of viable bacteria. In step A1, the sepiolite and deionized water are used in a ratio of (4-6) g:(70-80) mL. In step A2, the pretreated sepiolite, corn straw, tannic acid, deionized water and potassium hydroxide solution are used in a ratio of (3.5-3.8) g:(1.5-2) g:(0.1-0.3) g:(90-110) mL:(4.5-5.5) mL. In step A3, the sepiolite loaded with porous carbon, magnesium chloride hexahydrate solution and sodium hydroxide are used in a ratio of (4-5) g:(45-55) mL:(0.4-0.6) g. In step A4, the hydroxypropyl methyl cellulose, modified sepiolite, deionized water, tea polyphenol and ferric chloride are used in a ratio of (3.5-4.5) g:(1-2) g:(45-55) mL:(2-2.4) g:(0.1-0.3) g.

2. The high-efficiency microbial compound fertilizer according to claim 1, characterized in that, The microbial component is prepared by the following steps:

3. The high-efficiency microbial compound fertilizer according to claim 1, characterized in that, The compound filler is prepared by the following steps:

4. The high-efficiency microbial compound fertilizer according to claim 1, characterized in that, A1. The sepiolite and deionized water are mixed and stirred uniformly, and then the mixture is subjected to hydrothermal treatment at 150-200 DEG C for 3-7 hours under a pressure of 1-1.5 MPa, and then cooled to room temperature, filtered, washed and dried to obtain pretreated sepiolite; 5. The high-efficiency microbial compound fertilizer according to claim 1, characterized in that, A2. The pretreated sepiolite, corn straw and tannic acid are added to deionized water, and then subjected to stirring reaction, filtration, washing and drying, and then a potassium hydroxide solution is added, inert gas is introduced, and carbonization is carried out at 750-850 DEG C for 3-5 hours, and then the mixture is cooled to room temperature, filtered, washed and dried to obtain sepiolite loaded with porous carbon; 6. The high-efficiency microbial compound fertilizer according to claim 1, characterized in that, A3. The sepiolite loaded with porous carbon and a magnesium chloride hexahydrate solution are mixed and stirred uniformly, and then sodium hydroxide is added, and then the mixture is subjected to stirring, filtration, washing and drying, and then calcination is carried out at 450-550 DEG C for 4-6 hours, and then the mixture is cooled to room temperature to obtain modified sepiolite; A4. The hydroxypropyl methyl cellulose and modified sepiolite are added to deionized water, and then the mixture is stirred uniformly, and then tea polyphenol and ferric chloride are added, and then the mixture is stirred to a gel state, and then the gel is washed and freeze-dried to obtain the compound filler; The organic matter powder is selected from any one of sawdust, bran and straw; The compound bacterial strain is obtained by mixing Bacillus subtilis, Bacillus megaterium and Azotobacter chroococcum according to a ratio of (5-10):(5-10):(1-3) of the number of viable bacteria. In step A1, the sepiolite and deionized water are used in a ratio of (4-6) g:(70-80) mL. In step A2, the pretreated sepiolite, corn straw, tannic acid, deionized water and potassium hydroxide solution are used in a ratio of (3.5-3.8) g:(1.5-2) g:(0.1-0.3) g:(90-110) mL:(4.5-5.5) mL. In step A3, the sepiolite loaded with porous carbon, magnesium chloride hexahydrate solution and sodium hydroxide are used in a ratio of (4-5) g:(45-55) mL:(0.4-0.6) g. In step A4, the hydroxypropyl methyl cellulose, modified sepiolite, deionized water, tea polyphenol and ferric chloride are used in a ratio of (3.5-4.5) g:(1-2) g:(45-55) mL:(2-2.4) g:(0.1-0.3) g. The microbial component is prepared by the following steps: The complex bacteria is inoculated into a seed culture medium with an inoculation amount of 2-5%, and is cultured at 30-32℃ and 180-200rpm for 3-5 days, and the fermentation liquor is collected, diluted with clean water, mixed with organic matter powder, dried, and scattered into powder to obtain a microbial component.

7. The high-efficiency microbial compound fertilizer according to claim 6, characterized in that, The seed culture medium formula is: glucose 15-20g / L, amino acid 8-10g / L, magnesium sulfate 0.4-0.5g / L, potassium dihydrogen phosphate 0.4-0.5g / L, and the pH value of the culture medium is 7.0-7.

2.

8. A method for preparing the high-efficiency microbial compound fertilizer according to any one of claims 1-7, characterized in that, The preparation steps include the following: S1. Urea, monoammonium phosphate, diammonium phosphate, potassium dihydrogen sulfate, potassium sulfate and calcium-magnesium phosphate fertilizer are mixed and stirred at 60-80r / min for 5-10min to obtain a mixture; S2. The microbial component, the complex filler and the mixture are mixed and stirred at 60-80r / min for 5-10min, granulated, dried at 65-75℃ for 20-30min, screened, packaged, and a microbial compound fertilizer is obtained.

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