Composition with effects of controlling oil, removing acnes and inhibiting bacteria as well as preparation method and application of composition

Abstract

The invention discloses a composition with effects of controlling oil, removing acne and inhibiting bacteria, the composition is prepared from the following components in parts by weight: 10-50 parts of a composite micelle carrier, 0.1-10 parts of ampelopsis grossedentata extract and 0.1-50 parts of panthenol, and the invention also provides a preparation method and application of the composition. According to the composition provided by the invention, the chlorophyll copper and the vegetable fat are reasonably matched, the solubility and bioavailability of the composition are improved through a process technology, the biological activity of the composition can be effectively protected, and the composition has good oil-controlling, acne-removing and antibacterial effects and has a wide market prospect.

Description

Technical Field

[0001] The invention discloses a composition, a preparation method and an application thereof, and specifically, a composition with oil-controlling, acne-removing and antibacterial effects, a preparation method and an application thereof, belonging to the technical field of cosmetics. Background Art

[0002] Acne, also known as acne, is a common chronic inflammatory skin disease affecting the sebaceous follicles. It is closely linked to factors such as excessive sebum secretion, blocked ducts, bacterial infection, and inflammatory responses. With the accelerating pace of life and increasing environmental pressures, the incidence of acne is on the rise, and the age range of onset is gradually expanding.

[0003] There are numerous products on the market for the treatment and improvement of acne, but many suffer from ineffectiveness, irritation, or contain harmful chemicals. Plant oils and fats, due to their natural green color, mildness, and diverse bioactive components, have garnered widespread attention in the cosmetics field. Copper chlorophyllin exhibits some bioactivity, but when used alone, it suffers from poor solubility and compatibility, limiting its effectiveness.

[0004] Therefore, it is very necessary to develop a composition that rationally combines copper chlorophyllin with plant oils and fats, improves their solubility and bioavailability through specific process technologies, effectively protects their biological activity, and has good oil-controlling, acne-removing and antibacterial effects. Summary of the Invention

[0005] In response to the above-mentioned shortcomings, the purpose of the present invention is to provide a composition that rationally combines copper chlorophyllin with plant oils and fats, and improves their solubility and bioavailability through specific process technologies, which can effectively protect their biological activity and has good oil-control, acne-removing and antibacterial effects. A preparation method and application of the composition are also provided.

[0006] To this end, the first technical solution provided by the present invention is as follows:

[0007] A composition with oil-controlling, acne-removing and antibacterial effects consists of the following components in parts by weight: 10-50 parts of a composite micelle carrier, 0.1-10 parts of an ampelopsis serrulate extract and 0.1-50 parts of panthenol.

[0008] Furthermore, the composite micelle carrier consists of active substances, glyceride, silk fibroin, glycerol and water.

[0009] Furthermore, the active ingredient consists of copper chlorophyllin and sunflower seed oil.

[0010] Furthermore, the mass ratio of copper chlorophyllin to sunflower seeds in the active ingredient is 3:17.

[0011] Furthermore, the mass ratio of the active substance, glyceride, silk fibroin, glycerol and water in the composite micelle carrier is 1:2:2:(20-80):(20-80).

[0012] Furthermore, the mass ratio of the Ampelopsis grossedentata extract to panthenol is 1:(1-5).

[0013] The second technical solution provided by the invention is: a method for preparing a composition with oil-controlling, acne-removing and antibacterial effects, comprising the following steps:

[0014] A. Stir copper chlorophyllin, sunflower seed oil, and glyceryl (ethylhexanoate) at 45-50° C. using a stirrer at 500-800 rpm for 2-5 min until completely dissolved and clear, to obtain an oil phase;

[0015] B. Stir water, glycerol, and fibroin at 55-60° C. with a stirrer at 500-800 rpm for 5-10 minutes until completely dissolved to obtain an aqueous phase;

[0016] C. Cool the aqueous phase obtained in step B to 45-50° C., slowly add the oil phase obtained in step A to the oil phase obtained in step B while stirring, turn to homogenization and shearing at a speed of 8000-15000 rpm, and then perform high-pressure homogenization in an ultra-high pressure microfluidizer at a homogenization pressure of 20000-28000 PSI, circulate homogenization 2-5 times, and control the discharge temperature at 10-15° C. to obtain a composite micellar carrier;

[0017] D. In step C, the speed of adding the oil phase to the water phase is 5-10 mL / min, and the stirring speed is 500-800 rpm;

[0018] E. The obtained composite micellar carrier is stirred at 500-800 rpm for 2-5 minutes at room temperature, and is mixed evenly with the Ampelopsis dentata extract and panthenol to obtain a composition with oil-controlling, acne-removing and antibacterial effects.

[0019] The third technical solution provided by the invention is: an application of the above composition, wherein the composition is used in the preparation of cosmetics.

[0020] Furthermore, the cosmetic is a skin external preparation prepared by using the composition with anti-photodamage efficacy as an active component and adding conventional pharmaceutical or cosmetic excipients or auxiliary ingredients.

[0021] Furthermore, the composition is added in an amount of 0.01-20% in cosmetics.

[0022] Furthermore, the composition is added to cosmetics in an amount of 0.1-15%.

[0023] Furthermore, it is characterized in that the cosmetics are one or more of lotion, essence, cream, mask, and gel.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] (1) The present invention obtains a composite micelle carrier by treating copper chlorophyllin, sunflower seed oil, glycerol (ethylhexanoate), silk fibroin, glycerol and water with ultra-high pressure microjet homogenization technology, thereby solving the problem that oil-soluble copper chlorophyllin and water-soluble ampelopsis serrulata extract and panthenol cannot be mixed and applied. Ultra-high pressure microjet technology is a nano-carrier encapsulation technology suitable for continuous scale-up production, which can evenly encapsulate these components in tiny carriers to form stable nano-scale carriers. This treatment method not only improves the stability of these components, but also enables them to better combine with water-soluble components such as ampelopsis serrulata extract and panthenol, thereby achieving high-efficiency performance of the composition. Specifically, under an ultra-high pressure environment, copper chlorophyllin, sunflower seed oil, glycerol (ethylhexanoate) and silk fibroin are dispersed in a specific solvent, and are passed through a tiny special geometric structure model at an extremely high speed through a microjet device. Under the action of instantaneous high-pressure impact and shear force, a composite micelle carrier is formed.

[0026] (2) The composite micellar carrier of the present invention is compounded with the extract of Agave serrata and panthenol in a specific ratio, and the result is a mutual synergistic and mutually promoting effect, with an effect of 1+1>2, and has the best natural green and safe oil control, acne removal and antibacterial effect. The natural green anti-inflammatory and antibacterial effect of chlorophyllin copper, the regulation of oil secretion by the extract of Agave serrata and the synergistic effect with the panthenol component can effectively reduce the oil secretion of the skin, eliminate acne, inhibit bacterial growth, and improve the overall condition of oily skin. Ingredients such as panthenol and fibroin can replenish moisture and provide nourishment to the skin, so that the skin remains hydrated and smooth while controlling oil, removing acne and inhibiting bacteria, and improving the skin texture. With the addition of ingredients such as fibroin and the mild formula of the entire composition, it has good biocompatibility and is not prone to causing adverse reactions such as skin allergies.

[0027] (3) The present invention controls the average particle size of the composite micelle carrier to be between 100nm and 150nm and the discharge temperature of the ultra-high pressure microfluidizer technology to be below 15°C, which can effectively protect the copper chlorophyllin from being affected by the high temperature during the high-pressure treatment and reducing its activity. The ultra-high pressure microfluidization technology encapsulation treatment greatly improves the stability of the copper chlorophyllin, glycerol (ethylhexanoate) ester and silk fibroin, making them less likely to undergo chemical reactions when mixed with other ingredients, and can maintain their activity for a long time, thus ensuring the quality and efficacy stability of the product.

[0028] (4) The process technology adopted by the present invention is suitable for continuous scale-up production and has the effect of significantly improving the production efficiency of traditional nanocarriers, which can effectively achieve the company's goal of reducing costs and increasing efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] The present invention will be further explained below in conjunction with the accompanying drawings and examples:

[0030] Figure 1 This is a graph showing the average particle size of the composition with oil-controlling, acne-removing and antibacterial effects in Example 1 of the present invention;

[0031] Figure 2 This is the Zeta potential diagram of the composition with oil-controlling, acne-removing and antibacterial effects in Example 1 of the present invention. DETAILED DESCRIPTION

[0032] The following further describes the claims of the present invention in detail in conjunction with specific implementation methods, but does not constitute any limitation to the present invention. Any limited modifications made by anyone within the scope of protection of the claims of the present invention are still within the scope of protection of the claims of the present invention.

[0033] A composition with oil-controlling, acne-removing and antibacterial effects is prepared from the following raw materials in percentage by weight: 0.3g copper chlorophyll, 1.7g sunflower seed oil, 4g glyceryl (ethylhexanoate), 4g silk fibroin, 50g glycerol, 30g water, 5g ampelopsis serrulate extract, and 5g panthenol.

[0034] The composition of this embodiment is prepared by the following preparation method:

[0035] Step 1: stirring copper chlorophyllin, sunflower seed oil, and glyceryl (ethylhexanoate) at 45° C. and 800 rpm for 5 minutes until completely dissolved and clear, to obtain an oil phase;

[0036] Step 2: Stir water, glycerol, and fibroin at 55° C. and 800 rpm for 10 min until completely dissolved to obtain an aqueous phase;

[0037] Step 3: Cool the aqueous phase obtained in step 2 to 45°C, slowly add the oil phase in step 1 to the aqueous phase obtained in step 2 while stirring, add the oil phase to the aqueous phase at a rate of 10 mL / min, stir at a rate of 800 rpm, switch to homogenization shearing at a speed of 15000 rpm, and then perform high-pressure homogenization by an ultra-high pressure microfluidizer at a homogenization pressure of 25000 PSI, cycle homogenization 5 times, and control the discharge temperature at 10°C to obtain a composite micelle carrier.

[0038] Step 4: Stir the obtained composite micelle carrier at 800 rpm for 5 minutes at room temperature, and mix it evenly with the Ampelopsis dentata extract and panthenol to obtain a composition with oil-controlling, acne-removing and antibacterial effects.

[0039] The composition obtained in this example has the effects of oil control, acne treatment and antibacterial properties. The average particle size is 125.1 nm, the polydispersity index (PDI) is 0.100, and the zeta potential is -37.95 mV (see Figure 1 and Figure 2 ).

[0040] Example 2

[0041] A composition with oil-controlling, acne-removing and antibacterial effects is prepared from the following raw materials in percentage by weight: 0.2g copper chlorophyll, 1.13g sunflower seed oil, 2.66g glyceryl (ethylhexanoate), 2.66g silk fibroin, 20g glycerol, 67.35g water, 1g Ampelopsis glauca extract, and 5g panthenol.

[0042] The composition of this embodiment is prepared by the following preparation method:

[0043] Step 1: stirring copper chlorophyllin, sunflower seed oil, and glyceryl (ethylhexanoate) at 45° C. and 800 rpm for 5 minutes until completely dissolved and clear, to obtain an oil phase;

[0044] Step 2: Stir water, glycerol, and fibroin at 60° C. and 500 rpm for 8 minutes until completely dissolved to obtain an aqueous phase;

[0045] Step 3: Cool the aqueous phase obtained in step 2 to 50°C, slowly add the oil phase in step 1 to the aqueous phase obtained in step 2 while stirring, add the oil phase to the aqueous phase at a rate of 8 mL / min, stir at a rate of 500 rpm, switch to homogenization shearing at a speed of 8000 rpm, and then perform high-pressure homogenization by an ultra-high pressure microfluidizer at a homogenization pressure of 28000 PSI, cycle homogenization twice, and control the discharge temperature at 15°C to obtain a composite micelle carrier.

[0046] Step 4: Stir the obtained composite micelle carrier at 500 rpm for 3 minutes at room temperature, and mix it evenly with the Ampelopsis dentata extract and panthenol to obtain a composition with oil-controlling, acne-removing and antibacterial effects.

[0047] Example 3

[0048] A composition with oil-controlling, acne-removing and antibacterial effects is prepared from the following raw materials in percentage by weight: 0.1g copper chlorophyll, 0.565g sunflower seed oil, 1.33g glyceryl (ethylhexanoate), 1.33g silk fibroin, 62.675g glycerol, 30g water, 1g ampelopsis serrulate extract, and 3g panthenol.

[0049] The composition of this embodiment is prepared by the following preparation method:

[0050] Step 1: stirring copper chlorophyllin, sunflower seed oil, and glyceryl (ethylhexanoate) at 48° C. and 700 rpm for 2 min until completely dissolved and clear, to obtain an oil phase;

[0051] Step 2: Stir water, glycerol, and fibroin at 58° C. and 700 rpm for 5 minutes until completely dissolved to obtain an aqueous phase;

[0052] Step 3: Cool the aqueous phase obtained in step 2 to 48°C, slowly add the oil phase in step 1 to the aqueous phase obtained in step 2 while stirring, add the oil phase to the aqueous phase at a rate of 5 mL / min, stir at a rate of 700 rpm, switch to homogenization shearing at a speed of 12000 rpm, and then perform high-pressure homogenization by an ultra-high pressure microfluidizer at a homogenization pressure of 20000 PSI, cycle homogenization 4 times, and control the discharge temperature at 12°C to obtain a composite micelle carrier.

[0053] Step 4: Stir the obtained composite micelle carrier at 700 rpm for 2 minutes at room temperature, and mix it evenly with the Ampelopsis dentata extract and panthenol to obtain a composition with oil-controlling, acne-removing and antibacterial effects.

[0054] Comparative Example 1

[0055] In this comparative example, the ultrahigh pressure microfluidizer treatment in Example 1 was replaced with homogenous stirring using conventional technology, while other aspects remained unchanged.

[0056] Comparative Example 2

[0057] In this comparative example, the copper chlorophyllin in Example 2 was removed and the remaining amount was supplemented with water, while other conditions remained unchanged.

[0058] Comparative Example 3

[0059] In this comparative example, the Ampelopsis grossedentata extract in Example 2 was removed and the remaining amount was supplemented with water, while other contents remained unchanged.

[0060] Comparative Example 4

[0061] In this comparative example, the discharge temperature of the material treated by the ultrahigh pressure microfluidizer in Example 1 was not controlled, and other parameters were kept unchanged.

[0062] Comparative Example 5

[0063] In this comparative example, the silk fibroin in Example 2 was replaced with phospholipid, a more commonly used membrane material in cosmetics, while other materials remained unchanged.

[0064] Table 1. Composition of Examples and Comparative Examples

[0065]

[0066] In order to better illustrate the advantages of the present invention, the verification scheme provided by the present invention is given below:

[0067] Test Example 1: Accelerated stability performance test

[0068] This test is a one-month accelerated stability performance test of the compositions obtained in Examples 1-3 and Comparative Examples 1-5.

[0069] The composition prepared above was subjected to stability testing under normal temperature (room temperature), illumination (natural light), low temperature (-15°C), high temperature (45°C), high-low temperature cycling (45°C / -15°C for 24 hours each), and refrigeration (5°C). The data are recorded in Table 2.

[0070] Table 2. One-month accelerated stability performance test results of the composition

[0071] serial number normal temperature illumination Low temperature high temperature High and low temperature cycle refrigeration Example 1 No abnormalities No abnormalities No abnormalities No abnormalities No abnormalities No abnormalities Example 2 No abnormalities No abnormalities No abnormalities No abnormalities No abnormalities No abnormalities Example 3 No abnormalities No abnormalities No abnormalities No abnormalities No abnormalities No abnormalities Comparative Example 1 Separation and stratification Faded layering Separation and stratification Faded layering Faded layering Separation and stratification Comparative Example 2 No abnormalities No abnormalities No abnormalities No abnormalities No abnormalities No abnormalities Comparative Example 3 No abnormalities No abnormalities No abnormalities No abnormalities No abnormalities No abnormalities Comparative Example 4 Separation and stratification Faded layering Separation and stratification Faded layering Faded layering Separation and stratification Comparative Example 5 Separation and stratification Faded layering Separation and stratification Faded layering Faded layering Separation and stratification

[0072] As shown in Table 2, the composite micelle carrier obtained by ultra-high pressure microfluidization technology, using silk fibroin as the membrane material and controlling the discharge temperature can better solve the stability problem of the compounding of oil-soluble copper chlorophyllin with water-soluble Ampelopsis glauca extract and panthenol, and can better delay the fading problem of copper chlorophyllin. It also shows that the combination of silk fibroin and copper chlorophyllin with the specific ultra-high pressure microfluidization technology can better solve the stability problem of the compounding between copper chlorophyllin and Ampelopsis glauca extract.

[0073] Test Example 2: Human skin patch test

[0074] This test involved the compositions obtained in Examples 1-3 and Comparative Examples 1-5, prepared as 10% aqueous solutions. Following the human skin patch test specified in the 2022 Cosmetic Safety Technical Specifications, skin reactions were observed at 30 minutes (after the indentation disappeared), 24 hours, and 48 hours, and the results were recorded. The data are summarized in Table 3.

[0075] Table 3. Human skin patch test results of the composition

[0076] serial number 30min 24h 48h Example 1 Level 0, 30 people Level 0, 30 people Level 0, 30 people Example 2 Level 0, 30 people Level 0, 30 people Level 0, 30 people Example 3 Level 0, 30 people Level 0, 30 people Level 0, 30 people Comparative Example 1 Level 0, 30 people Level 0, 30 people Level 0, 30 people Comparative Example 2 Level 0, 30 people Level 0, 30 people Level 0, 30 people Comparative Example 3 Level 0, 30 people Level 0, 30 people Level 0, 30 people Comparative Example 4 Level 0, 30 people Level 0, 30 people Level 0, 30 people Comparative Example 5 Level 0, 30 people Level 0, 30 people Level 0, 30 people

[0077] As shown in Table 3, the composition is non-irritating when added in an amount of up to 10%, and can be used safely within this range.

[0078] Test Example 3: 5α-reductase inhibition experiment oil control test

[0079] This test is conducted on the compositions obtained in Examples 1-3 and Comparative Examples 1-5, which were prepared into 1% concentration samples.

[0080] This test method involves adding 1 mL of PBS solution to the blank control group, 1 mL of sample working solution to the sample group, 1 mL of finasteride working solution to the positive control group, and 1 mL of 5% ethanol to the enzyme tube. Add 1 mL of enzyme solution, 1 mL of NADPH solution, and 1 mL of testosterone solution to a test tube. Three parallel tubes are set up and gently shaken. Use a pipette to transfer 200 μL of the solution to a 96-well microplate. The plate is then placed in a microplate reader for analysis. The absorbance at 340 nm is measured as the first measurement, A0. After incubation at 37°C for 30 minutes, the plate is placed in a microplate reader for analysis. The absorbance at 340 nm is measured as the second measurement, A30. The 5α-reductase inhibition rate is also measured. The results are shown in Table 4.

[0081] Among them, 5α-reductase inhibition rate (%) = (1-(A sample 0-A sample 30) / (A enzyme 0-A enzyme 30)) × 100%, wherein A sample 0 is the absorbance value of the sample group measured for the first time (0 min), A sample 30 is the absorbance value of the sample group measured for the second time (30 min), A enzyme 0 is the average value of the absorbance measured in the enzyme tube for the first time (0 min), and A enzyme 30 is the average value of the absorbance measured in the enzyme tube for the second time (30 min).

[0082] Table 4. 5α-reductase inhibition test results of the composition

[0083]

[0084]

[0085] Note: If P>0.05, it means there is no significant difference, which is recorded as "ns"; if P<0.05, it means there is a significant difference, and 0.01<P≤0.05, it is recorded as "*"; 0.001<P≤0.01, it is recorded as "**"; 0.0001<P≤0.001, it is recorded as "***"; P≤0.0001, it is recorded as "****".

[0086] As can be seen from Table 4, compared with the blank control group, the 5α-reductase inhibition rate of the positive control group was 41.23%, which was significantly different from the 5α-reductase inhibition rates of 36.15%, 31.28% and 26.87% of the samples in Examples 1-3 at a concentration of 1%; there was no significant difference in the 5α-reductase inhibition rates of 1.56% and 2.37% of the samples in Comparative Examples 2 and 4 at the same concentration; there was a certain significant difference in the 5α-reductase inhibition rates of 12.14%, 13.75% and 18.29% of the samples in Comparative Examples 1, 3 and 5 at the same concentration. The results show that only by using ultra-high pressure microfluidization technology, silk fibroin as the membrane material, and controlling the discharge temperature to obtain a composite micelle carrier can the oil-control effect of the combination of copper chlorophyllin, Agave serrata extract and panthenol be efficiently exerted. It also shows that silk fibroin and copper chlorophyllin can exert a better synergistic oil-control effect only when they are in a specific proportion and under specific technical processes.

[0087] Test Example 4: Human Acne Treatment Efficacy Test

[0088] This test is based on the compositions obtained in Examples 1-3 and Comparative Examples 1-5. The compositions were added at a 10% dosage to the base formula of the essence lotion for comparative testing. The specific test method is as follows:

[0089] 4.1 Experimental Design

[0090] Single-blind, self-controlled before and after

[0091] 4.2 Test Purpose and Principle

[0092] 30 healthy Chinese subjects used the test samples for 14 consecutive days, and the acne volume of the subjects before and after use was compared to evaluate the acne-removing effect of the test samples.

[0093] 4.3. Usage

[0094] After cleansing in the morning and evening, take an appropriate amount of sample and pat it on the areas that need care (face, acne areas) until absorbed.

[0095] 4.4 Test Location

[0096] Cheeks and acne areas

[0097] 4.5 Test equipment and parameters

[0098] Table 5. Equipment and parameter table

[0099]

[0100] 4.6 Statistical Methods (Data Analysis)

[0101] SPSS statistical analysis software was used, and the Shapiro-Wilk test was used to test the significance of the normal distribution of the data improvement value. If Sig. (two-sided) > 0.01, it was normally distributed, and a paired t test was performed. If Sig. (two-sided) < 0.01, it was non-normally distributed, and a Wilcoxon test was performed.

[0102] If P>0.05, it means there is no significant difference, which is recorded as "ns";

[0103] If P < 0.05, it indicates a significant difference, and 0.01 ≤ P < 0.05, it is recorded as "*"; 0.001 ≤ P < 0.01, it is recorded as "**"; p < 0.001, it is recorded as "***".

[0104] Change rate (percentage) = (mean value of data after use - mean value of data before use) * 100% / mean value of data before use.

[0105] 4.7. Results and Analysis (the larger the negative value of the rate of change, the more obvious the effect)

[0106] Table 6. Change rate of acne volume of subjects

[0107]

[0108] As shown in Table 6, Examples 1-3 all significantly reduced acne volume and had a significant effect on acne, while Comparative Examples 1-5 did not significantly reduce acne volume and had no significant effect on acne. This indicates that the composite micelle carrier obtained through ultrahigh pressure microfluidization technology, using silk fibroin as the membrane material, and controlling the discharge temperature is required to effectively exert the acne-removing efficacy of the combination of copper chlorophyllin, Ampelopsis grossularis extract, and panthenol. It also shows that only when silk fibroin and copper chlorophyllin are used in a specific ratio and under specific technical processes can they exert a good synergistic and synergistic effect in removing acne.

[0109] Test Example 5: Antibacterial Experiment Test

[0110] The test was carried out in accordance with the "Test Method for Antibacterial Performance of Soluble Antibacterial (Inhibitory) Products" described in "Study on Evaluation Method of Antibacterial Efficacy of Antibacterial Cosmetics" (Daily Chemicals Science, Vol. 27, No. 12, December 2004).

[0111] 5.1、Operation steps:

[0112] (1) 24 h (bacteria) and 5 days (fungi) cultures of the experimental bacteria were washed with 0.03 mol / L PBS and prepared into a 10^6 cfu / mL bacterial suspension.

[0113] (2) Take 1 g of the test sample and add 0.1 mL of the above bacterial suspension to the sample and evenly spread and mix. After 20 minutes, add 9 mL of 0.03 mL PBS and mix thoroughly. After appropriate dilution, take 1 mL of each of 2 to 3 dilutions and place them in two parallel flat dishes. Then add the corresponding culture medium and mix and culture. Count the colonies. Repeat the above test 3 times and take the average. Set up a blank control.

[0114] Calculation: X = (AB) / A * 100%,

[0115] Where: X is the inhibition rate, %; A is the average number of colonies recovered from the control sample; B is the average number of colonies recovered from the test sample.

[0116] 5.2 Results Analysis

[0117] The results show that if the inhibition rate is 50-90%, the product has an antibacterial effect; if the inhibition rate is greater than 90%, the product has a strong antibacterial effect.

[0118] Acne treatment efficacy is evaluated at three levels: markedly effective, effective, and ineffective. A sample with an inhibition rate of ≥90% against all bacterial species is considered significantly effective in preventing and inhibiting acne. A sample with an inhibition rate of ≥50% to 90% against all bacterial species is considered moderately effective in preventing and inhibiting acne. A sample with an inhibition rate of <50% against all bacterial species is considered ineffective.

[0119] Table 7. Antibacterial test results

[0120]

[0121] As shown in Table 7, the composition samples of Examples 1-3 were effective against all four bacterial species, with inhibition rates exceeding 90%, demonstrating significant efficacy. This demonstrates that the composition samples prepared by the present invention can significantly and effectively prevent and inhibit the occurrence of acne. Comparative Examples 2 and 4 exhibited an inhibition rate of less than 50% against the four bacterial species, demonstrating no antibacterial or anti-acne effect. This indicates that the samples without the addition of copper chlorophyllin and without strict control of the ultrahigh-pressure microfluidization discharge temperature had no antibacterial or anti-acne effect. Comparative Examples 1, 3, and 5 exhibited an inhibition rate of 50% to 90% against the four bacterial species, demonstrating no significant anti-acne or antibacterial effect. This demonstrates that the composite micelle carrier obtained through ultrahigh-pressure microfluidization technology, using silk fibroin as the membrane material, and requiring controlled discharge temperature, can effectively exert the anti-acne and antibacterial efficacy of the combination of copper chlorophyllin, Ampelopsis glauca extract, and panthenol. This also demonstrates that only when silk fibroin and copper chlorophyllin are combined in a specific ratio and using a specific process can they achieve a synergistic and potent anti-acne and antibacterial effect.

[0122] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be considered as equivalent replacement methods and are included in the scope of protection of the present invention.

Claims

1. A composition with oil-controlling, acne-removing and antibacterial effects, characterized in that: The invention is composed of the following components in parts by weight: 10 to 50 parts of composite micelle carrier, 0.1 to 10 parts of Ampelopsis grossedentata extract and 0.1 to 50 parts of panthenol.

2. The composition according to claim 1, characterized in that The composite micelle carrier consists of active substances, glyceride, silk fibroin, glycerol and water.

3. The composition according to claim 1, characterized in that The actives consist of copper chlorophyllin and sunflower seed oil.

4. The composition according to claim 1, characterized in that The mass ratio of copper chlorophyllin to sunflower seeds in the active ingredient is 3:

17.

5. The composition according to claim 1, characterized in that The mass ratio of the active substance, glyceride, silk fibroin, glycerol and water in the composite micelle carrier is 1:2:2:(20-80):(20-80).

6. The composition according to claim 1, characterized in that The mass ratio of the Ampelopsis grossedentata extract to panthenol is 1:(1-5).

7. A method for preparing a composition having oil-controlling, acne-removing and antibacterial effects according to any one of claims 1 to 6, characterized in that: It consists of the following steps: A. Stir copper chlorophyllin, sunflower seed oil, and glyceryl (ethylhexanoate) at 45-50° C. using a stirrer at 500-800 rpm for 2-5 min until completely dissolved and clear, to obtain an oil phase; B. Stir water, glycerol, and fibroin at 55-60° C. with a stirrer at 500-800 rpm for 5-10 minutes until completely dissolved to obtain an aqueous phase; C. Cool the aqueous phase obtained in step B to 45-50° C., slowly add the oil phase obtained in step A to the oil phase obtained in step B while stirring, turn to homogenization and shearing at a speed of 8000-15000 rpm, and then perform high-pressure homogenization in an ultra-high pressure microfluidizer at a homogenization pressure of 20000-28000 PSI, circulate homogenization 2-5 times, and control the discharge temperature at 10-15° C. to obtain a composite micellar carrier; D. In step C, the speed of adding the oil phase to the water phase is 5-10 mL / min, and the stirring speed is 500-800 rpm; E. The obtained composite micellar carrier is stirred at 500-800 rpm for 2-5 minutes at room temperature, and is mixed evenly with the Ampelopsis dentata extract and panthenol to obtain a composition with oil-controlling, acne-removing and antibacterial effects.

8. Use of a composition having oil-controlling, acne-removing and antibacterial effects according to any one of claims 1 to 6 in the preparation of cosmetics, characterized in that: The cosmetic is a skin external preparation prepared by using the composition with oil-controlling, acne-removing and antibacterial effects as an active component and adding conventional pharmaceutical or cosmetic excipients or auxiliary ingredients.

9. The use according to claim 8, characterized in that The composition is added in an amount of 0.01-20% in cosmetics.

10. The use according to claim 8, characterized in that The cosmetics are one or more of lotion, essence, cream, mask, and gel.

Images