Vertical flow device and method for quantitatively detecting pathogenic microorganisms

Abstract

The invention provides a vertical flow device and method for quantitatively detecting pathogenic microorganisms, and belongs to the field of biological detection. Comprising a container body and a heating body, the container body is provided with a reaction cavity; the heating body is provided with an inner-layer shell, an outer-layer shell and a self-adaptive heating assembly, a heat generation cavity is formed between the inner-layer shell and the outer-layer shell and is divided into an upper cavity and a lower cavity, a first raw material is arranged in the upper cavity, a second raw material is put in the lower cavity, and the first raw material and the second raw material react to generate heat; the self-adaptive heating assembly automatically changes the heat transfer efficiency according to the temperature; one side of the bimetallic strip is connected with the inner-layer shell, the other side of the bimetallic strip is movably arranged in the gap, and the bimetallic strip has the working state of being close to / away from the container body and switched along with the temperature. According to the device, a closed reaction module and a self-heating system are integrated, and the key pain points of traditional detection equipment in the aspects of biological safety and portability are solved. The fully-closed design effectively prevents pathogen diffusion, the self-heating function ensures stable operation in a power-free environment, and the device is especially suitable for base-level medical treatment, epidemic emergency, field operation and other scenes.

Description

Technical Field

[0001] The present invention relates to the field of biological detection technology, and in particular to a vertical flow device and method for quantitatively detecting pathogenic microorganisms. Background Art

[0002] Traditional detection methods for pathogenic microbial infections rely primarily on laboratory culture and biochemical identification techniques. The core process involves sample collection, constant-temperature culture, and subsequent biochemical analysis. Despite the gradual miniaturization of detection technology in recent years, significant limitations persist in practical applications. First, it relies on a continuous power supply, making it difficult to maintain continuous operation in the field or during power outages following disasters. Second, insufficient integration results in the large size of the entire system due to the presence of essential components such as culture dishes, pharyngeal swabs, and temperature control modules, making it inconvenient to use in the field and unable to perform batch testing. These technical limitations severely restrict its rapid application in resource-constrained environments such as accident sites and primary healthcare settings. Summary of the Invention

[0003] In response to the shortcomings of the existing technology, the present invention proposes a vertical flow device and method for quantitative detection of pathogenic microorganisms to solve the technical problem that the detection device in the existing technology is large in size and not convenient for rapid batch detection.

[0004] The technical solution adopted by the present invention is a vertical flow device and method for quantitatively detecting pathogenic microorganisms.

[0005] Among them, a vertical flow device for quantitative detection of pathogenic microorganisms includes a container body and a heating body;

[0006] The container body is provided with a reaction chamber, and the reaction chamber can be opened;

[0007] The heating body includes an inner shell, an outer shell and an adaptive heating assembly. The inner side of the inner shell is a heating chamber for inserting the container body. The space between the inner shell and the outer shell is a heat generating chamber. The heat generating chamber is divided into an upper chamber and a lower chamber. A first raw material is placed in the upper chamber. A second raw material can be added to the lower chamber to generate heat.

[0008] After the container body is inserted into the heating chamber, there is still a gap between the side walls, and the adaptive heating component is located in the gap and automatically changes the heat transfer efficiency according to the temperature;

[0009] The adaptive heating component includes a bimetallic strip, one side of which is connected to the inner shell, and the other side is movably arranged in the gap. The bimetallic strip includes a working state of approaching or moving away from the container body according to temperature switching.

[0010] Optionally, the adaptive heating component also includes a heat transfer plate, which is arranged in the heating chamber, the rear side and / or bottom end of the heat transfer plate is connected to the inner shell, one side or both sides of the heat transfer plate are connected to the bimetallic strip, and the free end of the bimetallic strip is along the circumferential direction of the heating chamber.

[0011] Optionally, a feeding speed control mechanism is provided between the upper cavity and the lower cavity, comprising a feeding hole, a sliding hole, a sliding rod and a plug;

[0012] The feeding hole connects the upper cavity and the lower cavity, the sliding hole intersects with the feeding hole, the sliding rod is arranged in the sliding hole, and the plug is arranged on the sliding rod. During the axial movement of the sliding rod, the blocking amount of the feeding hole by the plug changes accordingly.

[0013] Optionally, the sliding hole includes a sliding section and a threaded section;

[0014] The sliding section intersects with the feeding hole, and the plug is located in the sliding section;

[0015] The threaded section is matched with the thread of the sliding rod. When the sliding rod is screwed, the sliding rod moves axially accordingly.

[0016] Optionally, the sliding hole includes a limiting section in the middle and a reset section and an adjustment section at both ends;

[0017] The adjusting section faces one side of the bimetallic strip, intersects with the feeding hole, the plug is located in the adjusting section, and the end of the sliding rod passes through the inner shell and is located at the rear side of the bimetallic strip;

[0018] At one end of the reset section, a first limiting structure is provided on the sliding rod, and a second limiting structure is provided on the outer shell. An elastic member is provided between the first limiting structure and the second limiting structure.

[0019] Optionally, the second limiting structure is threadedly connected to the reset section.

[0020] Optionally, a raw material chamber is further provided on the container body at the periphery of the reaction chamber, and the position of the raw material chamber corresponds to the lower chamber;

[0021] When the container body is loaded into the heating body, the raw material cavity is communicated with the lower cavity.

[0022] Optionally, a sampling container is detachably provided on the container body at the tail end of the reaction chamber through a self-sealing structure;

[0023] The self-sealing structure comprises:

[0024] A protruding communicating interface is located on the sampling container, and a concave communicating interface is located in the reaction chamber and matches the protruding communicating interface. A shaft rod is slidably provided on the protruding communicating interface toward the concave communicating interface. A sealing piece is provided on the inner end of the shaft rod located at the sampling container, and an elastic piece is provided between the sealing piece and the protruding communicating interface. Initially, the sealing piece is attached to the inner side of the protruding communicating interface and seals the interface. When plugged in, the concave communicating interface lifts up the sealing piece and opens the protruding communicating interface.

[0025] Optionally, the first raw material is calcium oxide, and the second raw material is water.

[0026] The vertical flow method for quantitatively detecting pathogenic microorganisms uses the vertical flow device for quantitatively detecting pathogenic microorganisms as described above, and includes the following steps:

[0027] Sampling steps: Collect samples from the wound / target source;

[0028] Mixing step: adding a buffer solution and the sample into the reaction chamber of the container body and mixing them evenly;

[0029] Heating and heat preservation step: the container body is inverted and inserted into the inner shell of the heating body, the second raw material is put into the upper cavity to react with the first raw material to generate heat, and the heat is transferred to the container body through the bimetallic strip. When the temperature is too high, the bimetallic strip deforms away from the container body and reduces the heat transfer effect. When the temperature is too low, the bimetallic strip clings to the container body and improves the heat transfer effect. When the bimetallic strip is deformed, the bimetallic strip also cooperates with the elastic member to adjust the feeding speed of the first raw material.

[0030] Sampling steps: Place the container upright, press the sampling container from the tail end, and the liquid in the container enters the sampling container;

[0031] Testing steps: Take out the sampling container for testing. The sampling container is made of transparent material.

[0032] It can be seen from the above technical solution that the beneficial technical effects of the present invention are as follows:

[0033] The device is self-heating, compact, and portable, making it easier to use in resource-constrained environments and significantly improving the flexibility of on-site testing. Its batch testing capabilities enable it to efficiently handle large-scale sample testing, providing fast and efficient testing support for scenarios such as accident scenes and primary healthcare, effectively improving overall testing efficiency and practicality. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly describes the drawings required for the specific embodiments or the description of the prior art. Similar elements or parts are generally identified by similar reference numerals throughout the drawings. Elements or parts in the drawings are not necessarily drawn to scale.

[0035] Figure 1 A schematic cross-sectional view of the present invention as a whole;

[0036] Figure 2 is a schematic cross-sectional view of the heating body of the present invention;

[0037] Figure 3 It is a schematic cross-sectional view of the present invention in an inverted manner;

[0038] Figure 4 For the present invention Figure 3 A partial enlarged schematic diagram in the middle;

[0039] Figure 5 This is a schematic diagram of the feeding speed control mechanism of the present invention;

[0040] Figure 6 This is a schematic diagram of the reaction chamber and the sampling container of the present invention not being connected;

[0041] Figure 7 This is a schematic diagram of the connection between the reaction chamber and the sampling container of the present invention;

[0042] Figure 8 This is a schematic cross-sectional view of the present invention in a heating state;

[0043] Figure 9 This is a schematic diagram of the sampling container of the present invention;

[0044] Figure 10 It is a half-section schematic diagram of the heating body of the present invention.

[0045] Figure numerals: container body 1, reaction chamber 10, raw material chamber 11, concave connecting interface 12, heating body 2, inner shell 20, upper cavity 212, lower cavity 213, outer shell 22, second limiting structure 222, bimetallic strip 23, heat transfer plate 24, feeding hole 25, sliding hole 26, sliding rod 27, first limiting structure 271, plug 28, elastic member 29, sampling container 3, protruding connecting interface 30, shaft rod 31, sealing sheet 32, elastic member 33. DETAILED DESCRIPTION

[0046] The following embodiments of the technical solution of the present invention will be described in detail with reference to the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the present invention and are therefore only examples and are not intended to limit the scope of protection of the present invention.

[0047] It should be noted that, unless otherwise specified, the technical or scientific terms used in this application should have the common meanings understood by those skilled in the art to which the present invention belongs.

[0048] This embodiment provides a vertical flow device for quantitative detection of pathogenic microorganisms, wherein a possible embodiment is as follows: Figure 1 As shown:

[0049] The container body 1 and the heating body 2 can be separated or plugged into each other. Initially, the container body 1 is located at the bottom and the heating body 2 covers the upper side of the container body 1.

[0050] The container body 1 is provided with a reaction chamber 10, which can be opened. Figure 6 and Figure 7 A sealing plug 101 is provided on the top of the reaction chamber 10. The sealing plug 101 can be made of elastic rubber material to close or open the plug opening;

[0051] The heating body 2 includes an inner shell 20, an outer shell 22 and an adaptive heating component. The inner side of the inner shell 20 is a heating chamber for inserting the container body 1. The inner shell 20 and the outer shell 22 are connected to a heat generating chamber, which is divided into an upper chamber 212 and a lower chamber 213. The upper chamber 212 is provided with a first raw material, and the lower chamber 213 can be used to put a second raw material into the upper chamber 212. The first raw material and the second raw material react to generate heat. In one possible combination, the first raw material is calcium oxide and the second raw material is water. The reaction formula is CaO+H2O→Ca(OH)2. This reaction is exothermic and will release a large amount of heat, thereby heating the reaction chamber 10 through the inner shell 20 and the adaptive heating component, and adaptively adjusting the heating and heat preservation effect.

[0052] After container body 1 is inserted into the heating chamber, a gap remains between the sidewalls. The adaptive heating assembly is located within this gap and automatically changes its heat transfer efficiency based on the temperature. Specifically, the adaptive heating assembly includes a bimetallic strip 23. One side of the bimetallic strip 23 is connected to the inner shell 20, receiving heat from the inner shell 20. The other side is movably positioned within the gap. The bimetallic strip 23 can switch between operating states, moving closer to or farther from container body 1, depending on the temperature.

[0053] To facilitate understanding of the above embodiments, the following briefly introduces the device usage scenarios and usage process:

[0054] First, the container body 1 and the heating body 2 are separated. This step only requires the container body 1, such as Figure 6As shown, it is necessary to open the sealing plug 101 of the reaction chamber 10 and add a buffer solution thereto. A buffer solution is a chemical solution that can stabilize the pH value in an aqueous solution, usually consisting of a weak acid and its conjugate base or a weak base and its conjugate acid. In biochemistry and molecular biology experiments, buffer solutions play a vital role, especially in the present bacterial / viral nucleic acid detection scenario, where the buffer solution can stabilize the pH value of the solution, ensuring that enzymes (such as DNA polymerases) function under optimal pH conditions, thereby improving the accuracy and reliability of the detection, preventing nucleic acids from being degraded by nucleases, and protecting the integrity of DNA or RNA. During sample collection and pretreatment, the buffer solution helps to lyse cells, release nucleic acids, and remove inhibitors, thereby improving the efficiency and quality of nucleic acid extraction. In specific implementation, the type of buffer solution can be selected as needed, which is not the core innovation of the present invention.

[0055] Next, a sample is collected from the wound / target source and added to the reaction chamber 10 (buffer). Depending on the site, various methods and tools can be used to collect samples, such as using a syringe to collect samples from a liquid or using a cotton swab to collect samples from a wound. The collected sample is added to the buffer, and then the reaction chamber 10 is sealed with a sealing plug 101 and shaken to mix well.

[0056] Next, turn the container body 1 upside down and insert the reaction chamber 10 into the heating chamber (inner shell 20). Figure 3 As shown. Simultaneously, a second raw material is added to the lower chamber 213. It enters the upper chamber 212 through the hole between the lower chamber 213 and the upper chamber 212, where it reacts with the first raw material 2121 in the upper chamber 212, generating heat and thereby promoting the reaction of the bacterial / viral sample in the buffer solution. Generally, the heating and holding time may take up to an hour, and during this process, the temperature should not be too high.

[0057] After the heating and holding time is sufficient (nucleic acid amplification), the container body 1 is removed from the heating chamber (inner shell 20) and the sample solution after the reaction is observed (the shell of the container body 1 can be set to a transparent material). The result can be judged by naked eye, or the result can be judged by a graphic processing APP after taking a photo, or the sample picture can be uploaded to a remote machine for storage and expert judgment.

[0058] In the above embodiment, in order to ensure the heat preservation effect during the reaction process, the adaptive heating component is as follows Figure 10 As shown, it also includes a heat transfer plate 24, which is arranged in the heating chamber. The rear side and / or bottom end of the heat transfer plate 24 is connected to the inner shell 20 to receive heat ( Figure 10 In the figure, only the bottom end of the heat transfer plate 24 is connected to the inner shell 20, and the heat transfer plate 24 is connected to the bimetallic strip 23 on one or both sides along the circumference. The free end of the bimetallic strip 23 is along the circumferential direction of the heating cavity. The heat transfer plate 24 is preferably made of a metal material with high thermal conductivity.

[0059] In the above embodiment, the working principle of adaptive heating is:

[0060] Initially, the temperature is room temperature. After the container 1 is inserted into the heating chamber, the outer wall of the container 1 does not directly contact the side wall of the inner shell 20. Instead, heat is transferred indirectly to the inner shell 20 via the bimetallic strips 23. At lower temperatures, the multiple bimetallic strips 23 arranged in a circular array surround the container 1, achieving high heat transfer efficiency. As the heat generation reaction in the upper chamber 212 proceeds, the temperature of the inner shell 20 begins to rise, and the temperature of the heat transfer plate 24 also rises, causing the bimetallic strips 23 to heat up and transfer heat to the container 1. As the temperature continues to rise, the bimetallic strips 23 deform, gradually loosening their fit with the container 1 and reducing heat transfer efficiency. Conversely, as the temperature decreases, the bimetallic strips 23 reverse their deformation, gradually moving closer to the container 1, and heat transfer efficiency also gradually improving. This repetitive process creates a negative feedback loop between the bimetallic strips 23 and the heat transfer efficiency, ensuring that heat is transferred to the container 1 at a relatively appropriate rate.

[0061] In the above embodiment, in addition to indirect heat transfer between the container body 1 and the inner shell 20 via the bimetallic strip 23 on the sides, the container body 1 also directly transfers heat via the sealing plug 101 on its bottom. If heat transfer is only desired via the bimetallic strip 23, a recess can be provided on the back of the sealing plug 101 to reduce the contact surface between the back of the sealing plug 101 and the bottom of the inner shell 20, thereby inhibiting direct heat transfer. If heat transfer is primarily through the sealing plug 101, supplemented by temperature regulation via the bimetallic strip 23, the sealing plug 101 can be made of a material with good thermal conductivity.

[0062] In the above embodiment, the bimetallic strip is formed by laminating two layers of metals with different thermal expansion coefficients. When the temperature changes, the bimetallic strip bends and deforms due to the expansion difference, so that it can be attached to or separated from the container body 1.

[0063] In this embodiment, in order to further accurately control the insulation temperature, a feeding speed control mechanism is provided between the upper cavity 212 and the lower cavity 213. Figure 4 and Figure 5 , including a feeding hole 25, a sliding hole 26, a sliding rod 27 and a plug 28;

[0064] The feeding hole 25 connects the upper cavity 212 and the lower cavity 213, the sliding hole 26 intersects with the feeding hole 25, the sliding rod 27 is set in the sliding hole 26, and the plug 28 is set on the sliding rod 27. During the axial movement of the sliding rod 27, the blocking amount of the plug 28 on the feeding hole 25 changes accordingly. Since the second raw material (such as water) is added to the lower cavity 213, the second raw material automatically enters the upper cavity 212 through the feeding hole 25 under the action of gravity and reacts with the first raw material to generate heat. By controlling the sliding amount of the sliding rod 27 in the sliding hole 26, the blocking amount of the plug 28 on the feeding hole 25 can be controlled, and the feeding speed can also be changed accordingly, thereby controlling the heat generation reaction to proceed in an orderly manner and achieving a long-term heat preservation effect. At the same time, this adjustable method can prevent the temperature from being too high or too low, resulting in the reaction between the sample and buffer in the container body 1 not proceeding in an orderly manner as expected.

[0065] The beneficial effects of the device of the above embodiment include at least the following: adaptively adjusting the heat transfer efficiency through the bimetallic strip ensures a stable heating and heat preservation process, maintaining the container body 1 within an appropriate temperature range, avoiding excessive temperature fluctuations or even temperature fluctuations outside the range, improving reaction efficiency and ensuring reaction quality. Furthermore, the plug sliding mechanism can control the speed of heat-generating raw materials, achieving a long-term heat preservation effect, ensuring the stability of reaction conditions, and improving the reliability of test results. The synergistic effect of the bimetallic strip and the plug adjustment mechanism maintains the reaction temperature within a reasonable range from the perspectives of both heat generation and heat transfer.

[0066] Specifically, in order to achieve the above-mentioned purpose of controlling the sliding amount of the sliding rod 27 in the sliding hole 26, thereby controlling the blocking amount of the plug 28 on the feeding hole 25, and correspondingly controlling the feeding speed, thereby controlling the heat generation reaction rate, in one of the methods:

[0067] The sliding distance of the sliding rod 27 in the sliding hole 26 is manually adjusted. Specifically, the sliding hole 26 includes a sliding section and a threaded section. The sliding section intersects with the feeding hole 25, and the plug 28 is located in the sliding section. The threaded section is threadedly engaged with the sliding rod 27, and the outer end of the sliding rod 27 extends beyond the outer shell 22. When the sliding rod 27 is twisted, the sliding rod 27 moves axially accordingly.

[0068] In another way:

[0069] The sliding rod 27 automatically changes its sliding position according to the temperature change, thereby adjusting the speed of adding the reaction raw materials. Figure 4 and Figure 5The sliding hole 26 includes a central limiting section and two end sections, one for reset and the other for adjustment. The limiting section has the smallest diameter and mates with the sliding rod 27. The adjustment section faces the bimetallic strip 23 and intersects with the feeding hole 25. The plug 28 is located within the adjustment section. The end of the sliding rod 27 passes through the inner shell 20 and is located behind the bimetallic strip 23. When the temperature is too high, the deformation of the bimetallic strip 23 pushes the sliding rod 27, which in turn moves the plug 28 and blocks part of the feeding hole 25, reducing the feeding speed and heat generation.

[0070] At one end of the reset section, a first limiting structure 271 is provided on the sliding rod 27, and a second limiting structure 222 is provided on the outer shell 22. An elastic member 29 is provided between the first limiting structure 271 and the second limiting structure 222. When the temperature is too low, the bimetallic strip 23 deforms and returns to a contact state with the container body 1. Under the restoring force of the elastic member 29, the sliding rod 27 is pushed to move, thereby driving the plug 28 to move and partially open the feeding hole 25, increasing the feeding speed and the heat generation.

[0071] In the above embodiment, the second limiting structure 222 is threadedly connected to the reset section. By twisting the second limiting structure 222, the second limiting structure 222 moves along the axis, thereby changing the initial distance between the second limiting structure 222 and the first limiting structure 271, thereby adjusting the initial compression of the elastic member 29, that is, adjusting the initial elastic force of the elastic member 29. Accordingly, when the sliding rod 27 is pushed by the deformation of the bimetallic strip 23, the amount of its sliding is also different, and the degree of blocking the feeding hole 25 is also different. The method in the above embodiment enables the feeding speed control mechanism to adjust the amount of feeding speed according to temperature changes. For different scenarios, the feeding speed control mechanism has different degrees of response to temperature changes.

[0072] In this embodiment, a raw material chamber 11 is further provided on the outer periphery of the reaction chamber 10 on the container body 1, and the position of the raw material chamber 11 corresponds to the lower chamber 213; when the container body 1 is loaded with the heating body 2, the raw material chamber 11 is connected to the lower chamber 213. There are many ways to generate heat, and the calcium oxide and water mentioned above are just one possible option. When using this combination, calcium oxide is pre-loaded in the upper chamber 212, and water can be taken on site, added to the lower chamber 213 and enters the upper chamber 212 through the feeding hole 25 to react with calcium oxide to generate heat.

[0073] However, in some cases, the raw material combination for the heat generation reaction may be different and therefore cannot be obtained on site. In this case, the first raw material is pre-sealed in the upper cavity 212, and the second raw material can be pre-sealed in the raw material cavity 11 of the container body 1. When the reaction is required, the cavity wall of the corresponding area of ​​the upper cavity 212 and the lower cavity 213 can be punctured. Figure 3As shown. The puncturing tool (e.g., a needle) can be obtained from the outside or fixed in the lower cavity 213 and facing the container body 1. Once the container body 1 is inserted upside down into the heating cavity of the heating body 2 and then rotated relative to it, the partition wall of the raw material cavity 11 can be circumferentially scratched. Of course, to facilitate the scratching, a film material 105 can be used.

[0074] This embodiment can be found in Figure 6-Figure 9 On the container body 1, a sampling container 3 is detachably provided at the tail end of the reaction chamber 10 through a self-sealing structure;

[0075] The self-sealing structure includes: a protruding communication interface 30 located on the sampling container 3, a concave communication interface 12 located in the reaction chamber 10 and matching the protruding communication interface 30, a shaft 31 slidingly provided on the protruding communication interface 30 toward the concave communication interface 12, a sealing sheet 32 ​​provided at the inner end of the shaft 31 located at the sampling container 3, an elastic member 33 provided between the sealing sheet 32 ​​and the protruding communication interface 30, the elastic member 33 is fixed to both sides, and initially, the elastic member 33 allows the sealing sheet 32 ​​to stick to the inner side of the protruding communication interface 30 and seal the interface, such as Figure 9 and Figure 6 As shown, when plugged in, the concave communication interface 12 lifts the sealing piece 32 and opens the convex communication interface 30, so that the sampling container 3 and the reaction chamber 10 are connected, as shown in FIG. Figure 7 shown.

[0076] Before the heating reaction, the sampling container 3 is inserted into the rear end of the reaction chamber 10 but not completely. Figure 6 As shown, it cannot be connected at this time. Then open the sealing plug 101, put in the buffer solution and sample, and then close the sealing plug 101. Invert the container body 1 and insert it into the inner shell 20 (heating chamber) of the heating body 2. After the reaction is completed, Figure 8 As shown, press the sampling container 3 to fully insert it into the tail end of the reaction chamber 10. At this time, the self-sealing structure is connected, and then the device is turned over so that the sampling container 3 is located at the bottom, and the reacted solution enters the sampling container 3. Then, take out the sampling container 3 from the tail end of the reaction chamber 10, and the self-sealing structure automatically seals, as shown in FIG. Figure 9 As shown. At least a portion of the sampling container 3 is made of transparent material. At this time, relevant personnel can observe, photograph, or otherwise process the liquid in the sampling container 3. In the above embodiment, it is possible to effectively prevent interference during the sampling process (such as the cotton swab head used for sampling) from entering the sampling container 3 and affecting the observation and photography of the reaction products.

[0077] In conjunction with the embodiment of the above device, the vertical flow method for quantitative detection of pathogenic microorganisms is described below. The vertical flow device for quantitative detection of pathogenic microorganisms as described above is used, and the following steps are included:

[0078] Sampling steps: Collect wound samples by suctioning with a syringe or using a cotton swab;

[0079] Mixing step: putting the buffer solution and the sample into the reaction chamber 10 of the container body 1 and mixing them evenly, for example, directly putting a cotton swab soaked with the sample into the buffer solution and shaking it;

[0080] Heating and heat preservation steps: The container body 1 is inverted and inserted into the inner shell 20 of the heating body 2. The second raw material is added to the upper cavity 212 to react with the first raw material to generate heat. The heat is transferred to the container body 1 through the bimetallic strip 23. When the temperature is too high, the bimetallic strip 23 deforms away from the container body 1, reducing the heat transfer effect. When the temperature is too low, the bimetallic strip 23 clings to the container body 1 to improve the heat transfer effect. When the bimetallic strip 23 deforms, it also cooperates with the elastic member 29 to adjust the feeding speed of the first raw material, thereby controlling the heat generation.

[0081] Sampling steps: After a sufficient reaction time (generally 1 hour), place the container body 1 upright and press the sampling container 3 from the rear end (the sampling container 3 is not fully inserted initially). The self-sealing structure of the sampling container 3 automatically opens, and the liquid in the container body 1 enters the sampling container 3.

[0082] Testing Steps: Remove the sampling container 3 for testing. The transparent material allows for visual inspection. Alternatively, you can take a photo using a mobile phone graphics app to analyze the results or upload the data. If conditions permit, the sampling container 3 can be sent to a specialized laboratory for more comprehensive testing. It should be noted that conducting more comprehensive testing is not the focus of this solution; this solution aims to provide rapid on-site testing.

[0083] This device, combined with its method, solves the problems of how to keep the heat in the pre-treatment process before testing and how to reduce the size of the device through self-heating, significantly improving its practicality in resource-constrained environments. First, it gets rid of the dependence on a continuous power supply, allowing the equipment to continue working in the wild or in post-disaster power outage scenarios, ensuring the independence and reliability of the detection. Secondly, it improves the degree of integration and reduces the size of the equipment, making it more convenient for on-site use. In addition, the device is low-cost and small in size, and can be carried and used in batches, enhancing batch detection capabilities. These improvements together make the equipment more adaptable to resource-constrained environments such as accident sites and primary healthcare, effectively improving detection efficiency and practicality, and providing strong support for rapid response and response.

[0084] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some or all of the technical features therein. These modifications or replacements do not deviate the essence of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present invention, and they should all be included in the scope of the claims and description of the present invention.

Claims

1. A vertical flow device for quantitative detection of pathogenic microorganisms, characterized by: It comprises a container body (1) and a heating body (2); The container body (1) is provided with a reaction chamber (10), and the reaction chamber (10) is openable; The heating body (2) comprises an inner shell (20), an outer shell (22) and an adaptive heating component; the inner side of the inner shell (20) is a heating chamber for inserting the container body (1); a heat generating chamber is provided between the inner shell (20) and the outer shell (22); the heat generating chamber is divided into an upper chamber (212) and a lower chamber (213); a first raw material is provided in the upper chamber (212); a second raw material can be added to the lower chamber (213) into the upper chamber (212); the first raw material and the second raw material react to generate heat; After the container body (1) is inserted into the heating chamber, there is still a gap between the side walls, and the adaptive heating component is located in the gap; The adaptive heating component includes a bimetallic strip (23), one side of which is connected to the inner shell (20), and the other side is movably arranged in the gap, and the bimetallic strip (23) includes a working state of approaching or moving away from the container body (1) according to temperature switching.

2. The vertical flow device for quantitative detection of pathogenic microorganisms according to claim 1, characterized in that: The adaptive heating component also includes a heat transfer plate (24), which is arranged in the heating chamber, and the rear side and / or bottom end of the heat transfer plate (24) is connected to the inner shell (20), and one side or both sides of the heat transfer plate (24) are connected to the bimetallic strip (23), and the free end of the bimetallic strip (23) is along the circumferential direction of the heating chamber.

3. The vertical flow device for quantitative detection of pathogenic microorganisms according to claim 2, characterized in that: A feeding speed control mechanism is provided between the upper cavity (212) and the lower cavity (213), comprising a feeding hole (25), a sliding hole (26), a sliding rod (27) and a plug (28); The feeding hole (25) is connected to the upper cavity (212) and the lower cavity (213), the sliding hole (26) intersects with the feeding hole (25), the sliding rod (27) is arranged in the sliding hole (26), and the plug (28) is arranged on the sliding rod (27). During the axial movement of the sliding rod (27), the blocking amount of the feeding hole (25) by the plug (28) changes accordingly.

4. The vertical flow device for quantitative detection of pathogenic microorganisms according to claim 3, characterized in that: The sliding hole (26) includes a sliding section and a threaded section; The sliding section intersects with the feeding hole (25), and the plug (28) is located in the sliding section; The threaded section is threadably matched with the sliding rod (27); when the sliding rod (27) is screwed, the sliding rod (27) moves axially accordingly.

5. The vertical flow device for quantitative detection of pathogenic microorganisms according to claim 3, characterized in that: The sliding hole (26) comprises a limiting section in the middle and a reset section and an adjustment section at both ends; The adjusting section faces one side of the bimetallic strip (23), intersects with the feeding hole (25), the plug (28) is located in the adjusting section, and the end of the sliding rod (27) passes through the inner shell (20) and is located at the rear side of the bimetallic strip (23); At one end of the reset section, a first limiting structure (271) is provided on the sliding rod (27), a second limiting structure (222) is provided on the outer shell (22), and an elastic member (29) is provided between the first limiting structure (271) and the second limiting structure (222).

6. The vertical flow device for quantitative detection of pathogenic microorganisms according to claim 5, characterized in that: The second limiting structure (222) is threadedly connected to the reset section.

7. The vertical flow device for quantitative detection of pathogenic microorganisms according to claim 1, characterized in that: A raw material chamber (11) is further provided on the container body (1) at the periphery of the reaction chamber (10), and the position of the raw material chamber (11) corresponds to the lower chamber (213); When the container body (1) is loaded into the heating body (2), the raw material chamber (11) is connected to the lower chamber (213).

8. The vertical flow device for quantitative detection of pathogenic microorganisms according to claim 1, characterized in that: On the container body (1), a sampling container (3) is detachably provided at the tail end of the reaction chamber (10) via a self-sealing structure; The self-sealing structure comprises: A protruding communication interface (30) is located on the sampling container (3), and a concave communication interface (12) is located in the reaction chamber (10) and matches the protruding communication interface (30). A shaft (31) is slidably provided on the protruding communication interface (30) and faces the concave communication interface (12). A sealing sheet (32) is provided at the inner end of the shaft (31) located on the sampling container (3). An elastic member (33) is provided between the sealing sheet (32) and the protruding communication interface (30). Initially, the sealing sheet (32) is attached to the inner side of the protruding communication interface (30) and seals the interface. When plugged in, the concave communication interface (12) lifts the sealing sheet (32) and opens the protruding communication interface (30).

9. The vertical flow device for quantitative detection of pathogenic microorganisms according to claim 1, characterized in that: The first raw material is calcium oxide, and the second raw material is water.

10. A vertical flow method for quantitatively detecting pathogenic microorganisms, using the vertical flow device for quantitatively detecting pathogenic microorganisms according to claim 1, characterized in that: The following steps are involved: Sampling steps: Collect samples from the wound / target source; Mixing step: the reaction chamber (10) of the container body (1) is filled with a buffer solution and the sample and then mixed; Heating and heat preservation steps: the container body (1) is invertedly inserted into the inner shell (20) of the heating body (2), the second raw material is put into the upper cavity (212) to react with the first raw material to generate heat, and the heat is transferred to the container body (1) through the bimetallic strip (23); when the temperature is too high, the bimetallic strip (23) is deformed away from the container body (1) to reduce the heat transfer effect; when the temperature is too low, the bimetallic strip (23) is closely attached to the container body (1) to improve the heat transfer effect; when the bimetallic strip (23) is deformed, the bimetallic strip (23) also cooperates with the elastic member (29) to adjust the feeding speed of the first raw material; Sampling steps: placing the container body (1) upright, pressing the sampling container (3) from the rear end, and the liquid in the container body (1) enters the sampling container (3); Testing steps: taking out the sampling container (3) for testing, wherein the sampling container (3) is made of transparent material.

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