Agricultural biological compound enzyme preparation and preparation method thereof

Abstract

The invention relates to the technical field of enzyme preparations, in particular to an agricultural biological compound enzyme preparation and a preparation method thereof.The agricultural biological compound enzyme preparation is prepared from, by weight, 30-50 parts of compound enzyme, 5-8 parts of trehalose, 3-5 parts of glycerin, 15-20 parts of copolymer carrier, 5-8 parts of humic acid, 5-10 parts of bran, 3-5 parts of dipotassium phosphate and 85-124 parts of water. The compound enzyme contains various enzymes such as cellulase, lysozyme and protease, can synchronously decompose various organic substances such as cellulose, microbial cell walls and protein in soil and release nutrients such as nitrogen, phosphorus and potassium, has more comprehensive functions compared with a single enzyme preparation, and can meet the requirements of complex soil and crops in agricultural production.

Description

Technical Field

[0001] The present invention relates to the technical field of enzyme preparations, and in particular to an agricultural biological composite enzyme preparation and a preparation method thereof. Background Art

[0002] Traditional agricultural production has long relied on chemical fertilizers and pesticides, leading to problems such as soil compaction, excessive pesticide residues, and ecological imbalance, severely hampering sustainable agricultural development. As a green alternative, biocomplex enzyme preparations, through the synergistic action of multiple enzymes, can significantly improve nutrient utilization efficiency, promote crop growth and development, and enhance stress resistance, thereby effectively reducing the use of chemical fertilizers and pesticides. They have multiple functions, including decomposing organic matter, releasing soil nutrients, improving soil structure, and enhancing the quality and safety of agricultural products. Compared to single-function enzyme preparations, complex enzyme preparations can more systematically address the complex soil environment and crop needs in agricultural production.

[0003] However, enzyme preparations are highly sensitive to the environment, and enzyme activity is easily affected by the physical and chemical properties of the soil and climatic conditions. Changes in environmental factors such as soil pH, temperature, humidity, and heavy metal content will have a significant effect on their activity. In acidic or alkaline soil environments and extreme climatic conditions, enzymes are easily inactivated, resulting in poor stability of their action. In addition, enzyme preparations are highly dependent on the organic matter content of the soil, and their catalytic efficiency will be greatly reduced in poor soil environments. At the same time, the functional durability of enzyme preparations is insufficient. Most enzyme preparations degrade rapidly in the soil and cannot maintain activity in the soil for a long time. Frequent application is usually required, making it difficult to form a long-term soil microecological regulation effect. Summary of the Invention

[0004] In order to solve the problems mentioned in the above background technology, the present invention provides an agricultural biological composite enzyme preparation and a preparation method thereof.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] An agricultural biocomplex enzyme preparation comprising the following raw materials in parts by weight:

[0007] 30-50 parts of complex enzyme, 5-8 parts of trehalose, 3-5 parts of glycerol, 15-20 parts of copolymer carrier, 5-8 parts of humic acid, 5-10 parts of bran, 3-5 parts of dipotassium hydrogen phosphate and 85-124 parts of water.

[0008] Furthermore, based on the total weight of the complex enzyme, the enzyme components of the complex enzyme include: 8-12 parts of cellulase, 4-6 parts of lysozyme, 6-9 parts of transaminase, 4-6 parts of protease, 2-3 parts of oxidase and 2-3 parts of oxidoreductase.

[0009] Furthermore, the copolymer carrier comprises the following steps to prepare:

[0010] S1. Under nitrogen protection, hydroxyethyl acrylate and isoprene were added to a reactor pre-charged with N-methylpyrrolidone, and azobisisobutyronitrile was added. The reaction was stirred at controlled temperature for 6-8 hours. If necessary, a small amount of hydroquinone was added to terminate the reaction. After the reaction was completed, the mixture was cooled in an ice bath at 0°C to precipitate, filtered, washed three times with anhydrous ethanol, and dried at 60°C to constant weight to obtain a copolymer powder.

[0011] S2. Under nitrogen protection, add the copolymer powder to N-methylpyrrolidone, add propylene carbonate and aluminum acetylacetonate, heat in an oil bath, stir and react for 12-16 hours. After the reaction is completed, cool to 60-65°C, add 1% 0.1M hydrochloric acid solution, drop ice water into the reaction solution to precipitate, forming a milky white colloid, filter, wash with anhydrous ethanol three times, and dry at 60°C to constant weight to obtain a copolymer carrier.

[0012] Furthermore, in step S1, the mass ratio of hydroxyethyl acrylate, isoprene, N-methylpyrrolidone and azobisisobutyronitrile is (83-85):(16-18):(300-320):(1-2).

[0013] Furthermore, in step S2, the mass ratio of the copolymer powder, N-methylpyrrolidone, propylene carbonate and aluminum acetylacetonate is (100-105):(300-320):(18-22):(15-16).

[0014] Furthermore, in step S1, the temperature is controlled to 70-80° C., and the stirring speed is 100-200 rpm.

[0015] Furthermore, in step S2, the temperature of the oil bath is raised to 110-120° C., and the stirring speed is 200-300 rpm.

[0016] According to another aspect of the present invention, there is provided a method for preparing the above-mentioned agricultural biocomplex enzyme preparation, comprising the following steps:

[0017] Trehalose, glycerol and water are added to a mixing tank according to weight, and stirred until dissolved. At room temperature, a composite enzyme is slowly added and stirred for 30-40 minutes. A copolymer carrier and dipotassium hydrogen phosphate are added and stirred for adsorption for 2-3 hours. The pH value is adjusted. Humic acid and bran are added in sequence and high-speed shearing is performed for 10-20 minutes. The wet granules are formed by an extrusion granulator to obtain wet granules. The wet granules are spread on a dryer and dried for 50-60 minutes to control the moisture content to ≤8%. The granules are sieved through a 60-mesh sieve to obtain an agricultural biological composite enzyme preparation.

[0018] Furthermore, the stirring speed is 100-200 rpm, the pH value is adjusted to 6.8±0.2, the high-speed shearing speed is 600-700 rpm, and the drying temperature is 40-50°C.

[0019] Beneficial effects of the present invention:

[0020] 1. In the technical solution of the present invention, hydroxyethyl acrylate and isoprene form an amphiphilic molecular chain containing a hydroxyl polar segment and an olefin flexible segment under the action of an initiator. After coordination with aluminum acetylacetonate, the hydroxyl group forms an Al-O coordination bond with the aluminum ion, cross-linking the linear molecular chain into a porous network. Among them, the hydrophilic hydration layer formed by the polar hydroxyl group tightly wraps the enzyme active center, buffering the impact of soil pH fluctuations on the enzyme conformation; the dynamic reversible characteristics of the olefin flexible segment and the coordination bond can offset the thermal stress caused by temperature changes through segment deformation and bond reorganization, avoiding irreversible denaturation of enzyme molecules due to high or low temperatures. Compared with traditional enzyme preparations directly exposed to the soil environment, the composite enzyme of the present invention is fixed inside the carrier network and on the pore surface, which can significantly reduce the damage to enzyme activity caused by adverse factors such as heavy metal ions and extreme acid and alkali in the soil, and improve the stability of the enzyme preparation in complex field environments from the structural root.

[0021] 2. In the technical solution of the present invention, the copolymer carrier forms a nano- to micron-scale porous network through coordination cross-linking, which has an extremely high specific surface area and abundant active sites. Among them, hydroxyl groups and aluminum ions can actively adsorb polar substrates in the soil through hydrogen bonds, coordination bonds, etc., while the hydrophobic regions composed of olefin segments have an enrichment effect on non-polar substrates. This amphiphilic interface can form a local substrate concentration gradient on the carrier surface without relying on soil organic matter, and can maintain high reaction efficiency even in soils with low organic matter content. In addition, the pore structure of the three-dimensional network can allow small molecule substrates to diffuse freely to the enzyme active site, while limiting the loss of enzyme molecules, reducing the dependence of traditional enzyme preparations on soil organic matter.

[0022] 3. In the technical solution of the present invention, the complex enzyme contains multiple enzymes such as cellulase, lysozyme, and protease, which can simultaneously decompose various organic substances such as cellulose, microbial cell walls, and proteins in the soil, releasing nutrients such as nitrogen, phosphorus, and potassium. Compared with single enzyme preparations, it has more comprehensive functions and can meet the complex soil and crop needs in agricultural production. Transaminases and proteases are involved in the synthesis and transformation of amino acids in plants, and oxidases and oxidoreductases regulate redox balance, which can enhance plant metabolic functions, promote root development and photosynthesis, improve crop resistance, and achieve increased yield and quality. By decomposing organic matter, the soil porosity is increased, the air permeability and water retention are improved, and the problem of soil compaction is alleviated. At the same time, humic acid can further optimize the soil colloidal structure, enhance fertilizer retention capacity, and continuously improve soil fertility. DETAILED DESCRIPTION

[0023] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0024] Unless otherwise specified, the raw materials used in the present invention, such as hydroxyethyl acrylate (CAS No.: 818-61-1), isoprene (CAS No.: 78-79-5), propylene carbonate (CAS No.: 108-32-7), aluminum acetylacetonate (CAS No.: 13963-57-0), N-methylpyrrolidone (CAS No.: 872-50-4), and azobisisobutyronitrile (CAS No.: 78-67-1), are all commercially available.

[0025] Preparation Example 1

[0026] The copolymer carrier comprises the following steps to prepare:

[0027] S1. Under nitrogen protection, 83 g of hydroxyethyl acrylate and 16 g of isoprene were added to a reactor pre-charged with 300 g of N-methylpyrrolidone, and 1 g of azobisisobutyronitrile was added. The mixture was stirred at 70°C and 100 rpm for 6 h. After the reaction was completed, the mixture was cooled in an ice bath at 0°C to precipitate, filtered, washed three times with anhydrous ethanol, and dried at 60°C to constant weight to obtain a copolymer powder.

[0028] S2. Under nitrogen protection, add 100g of copolymer powder to 300g of N-methylpyrrolidone, add 18g of propylene carbonate and 15g of aluminum acetylacetonate, heat the oil bath to 110°C, and stir at 200rpm to react for 12h. After the reaction is completed, cool to 60°C, add 1% 0.1M hydrochloric acid solution, and drop ice water into the reaction solution to precipitate to form a milky white colloid. Filter, wash with anhydrous ethanol 3 times, and dry at 60°C to constant weight to obtain a copolymer carrier.

[0029] Preparation Example 2

[0030] The copolymer carrier comprises the following steps to prepare:

[0031] S1. Under nitrogen protection, 84 g of hydroxyethyl acrylate and 17 g of isoprene were added to a reactor pre-charged with 310 g of N-methylpyrrolidone, and 1.5 g of azobisisobutyronitrile was added. The mixture was stirred at 75°C and 150 rpm for 7 h. After the reaction was completed, the mixture was cooled in an ice bath at 0°C to precipitate, filtered, washed three times with anhydrous ethanol, and dried at 60°C to constant weight to obtain a copolymer powder.

[0032] S2. Under nitrogen protection, 102.5g of copolymer powder was added to 310g of N-methylpyrrolidone, 20g of propylene carbonate and 15.5g of aluminum acetylacetonate were added, the oil bath was heated to 115°C, and the reaction was stirred at 250rpm for 14h. After the reaction was completed, the mixture was cooled to 62°C, 1% 0.1M hydrochloric acid solution was added, and ice water was dropped into the reaction solution to precipitate to form a milky white colloid. The mixture was filtered, washed with anhydrous ethanol 3 times, and dried at 60°C to constant weight to obtain a copolymer carrier.

[0033] Preparation Example 3

[0034] The copolymer carrier comprises the following steps to prepare:

[0035] S1. Under nitrogen protection, 85 g of hydroxyethyl acrylate and 18 g of isoprene were added to a reactor pre-charged with 320 g of N-methylpyrrolidone, and 2 g of azobisisobutyronitrile was added. The mixture was stirred at 80°C and 200 rpm for 8 h. 0.05 g of hydroquinone was added to terminate the reaction. After the reaction, the mixture was cooled in an ice bath at 0°C to precipitate, filtered, washed three times with anhydrous ethanol, and dried at 60°C to constant weight to obtain a copolymer powder.

[0036] S2. Under nitrogen protection, 105g of copolymer powder was added to 320g of N-methylpyrrolidone, 22g of propylene carbonate and 16g of aluminum acetylacetonate were added, the oil bath temperature was raised to 120°C, and the reaction was stirred at 300rpm for 16h. After the reaction was completed, the mixture was cooled to 65°C, 1% 0.1M hydrochloric acid solution was added, and ice water was dropped into the reaction solution to precipitate to form a milky white colloid. The mixture was filtered, washed with anhydrous ethanol 3 times, and dried at 60°C to constant weight to obtain a copolymer carrier.

[0037] Example 1

[0038] A method for preparing an agricultural biocomplex enzyme preparation comprises the following steps:

[0039] 5 parts of trehalose, 3 parts of glycerol and 85 parts of water were added to a mixing tank by weight and stirred at 100 rpm until dissolved. At room temperature, 8 parts of cellulase, 4 parts of lysozyme, 6 parts of transaminase, 4 parts of protease, 2 parts of oxidase and 2 parts of oxidoreductase were slowly added and stirred at 100 rpm for 30 minutes. 15 parts of the copolymer carrier prepared in Preparation Example 1 and 3 parts of dipotassium hydrogen phosphate were added and stirred and adsorbed at 100 rpm for 2 hours. The pH value was adjusted to 6.6. 5 parts of humic acid and 5 parts of bran were added in sequence. The mixture was sheared at a speed of 600 rpm for 10 minutes and formed into wet granules by an extrusion granulator. The wet granules were spread on a dryer and dried at 40°C for 50 minutes. The moisture content was controlled to 8%. The mixture was sieved through a 60-mesh sieve to obtain an agricultural biocomposite enzyme preparation.

[0040] Example 2

[0041] A method for preparing an agricultural biocomplex enzyme preparation comprises the following steps:

[0042] 6.5 parts of trehalose, 4 parts of glycerol and 104.5 parts of water were added to a mixing tank by weight and stirred at 150 rpm until dissolved. At room temperature, 10 parts of cellulase, 5 parts of lysozyme, 7.5 parts of transaminase, 5 parts of protease, 2.5 parts of oxidase and 2.5 parts of oxidoreductase were slowly added, and stirred at 150 rpm for 35 minutes. 17.5 parts of the copolymer carrier prepared in Preparation Example 2 and 4 parts of dipotassium hydrogen phosphate were added, and adsorbed at a speed of 150 rpm for 2.5 hours. The pH value was adjusted to 6.8, and 6.5 parts of humic acid and 7.5 parts of bran were added in sequence. The mixture was sheared at a speed of 650 rpm for 15 minutes and formed into wet granules by an extrusion granulator. The wet granules were spread on a dryer and dried at 45°C for 55 minutes. The moisture content was controlled to 8%. The mixture was sieved through a 60-mesh sieve to obtain an agricultural bio-composite enzyme preparation.

[0043] Example 3

[0044] A method for preparing an agricultural biocomplex enzyme preparation comprises the following steps:

[0045] 8 parts of trehalose, 5 parts of glycerol and 124 parts of water were added to a mixing tank by weight and stirred at 200 rpm until dissolved. At room temperature, 12 parts of cellulase, 6 parts of lysozyme, 9 parts of transaminase, 6 parts of protease, 3 parts of oxidase and 3 parts of oxidoreductase were slowly added, and stirred at 200 rpm for 40 minutes. 20 parts of the copolymer carrier prepared in Preparation Example 3 and 5 parts of dipotassium hydrogen phosphate were added, and adsorbed at a speed of 200 rpm for 3 hours. The pH value was adjusted to 7.0, and 8 parts of humic acid and 10 parts of bran were added in sequence. The mixture was sheared at a speed of 700 rpm for 20 minutes and formed into wet granules by an extrusion granulator. The wet granules were spread on a dryer and dried at 50°C for 60 minutes. The moisture content was controlled to 8%. The mixture was sieved through a 60-mesh sieve to obtain an agricultural biocomposite enzyme preparation.

[0046] Comparative Example 1

[0047] The difference between this comparative example and Example 1 is that hydroxyethyl acrylate is used instead of the copolymer carrier prepared in Preparation Example 1, and the remaining steps are the same as those in Example 1.

[0048] Comparative Example 2

[0049] The difference between this comparative example and Example 2 is that isoprene is used instead of the copolymer carrier prepared in Preparation Example 2, and the remaining steps are the same as in Example 2.

[0050] Comparative Example 3

[0051] The difference between this comparative example and Example 3 is that aluminum acetylacetonate is used instead of the copolymer carrier prepared in Preparation Example 3, and the remaining steps are the same as those in Example 3.

[0052] With reference to GB / T 23527.4-2025 "Quality Requirements for Enzyme Preparations Part 4: Immobilized Glucose Isomerase Preparations", GB / T 23535-2009 "Lipase Preparations" and GB 20287-2006 "Agricultural Microbial Agents", 1.0 g of the samples prepared in Examples 1-3 and Comparative Examples 1-3 were added to 50 mL of 0.05 M phosphate buffer at pH 6.8, respectively. The mixture was shaken and extracted at 4°C for 2 hours, centrifuged at 8000 rpm for 10 minutes, and the supernatant was taken as the enzyme solution to be tested and stored at 4°C for later use. 0.5 mL of the enzyme solution to be tested was added to 0.5 mL of a 1% CMC-Na solution pre-dissolved in 0.05 M acetate buffer at a pH of 4.8, and the mixture was incubated at 50°C for 30 minutes. 1.5 mL of DNS reagent was immediately added, and the mixture was in a boiling water bath for 5 minutes. After cooling, the volume was adjusted to 10 mL. The OD value was measured at 540 nm. This was repeated three times. U / g was calculated using the following formula:

[0053]

[0054] Where U / g is the unit of enzyme activity; ΔC is the change in reducing sugar concentration in the reaction system (mg / mL); V is the total volume of the reaction system (mL); D is the sample dilution factor; t is the enzymatic reaction time (min); and m is the mass of the enzyme sample involved in the reaction (g). The results are shown in Table 1:

[0055] Table 1. Cellulase activity test results of Examples 1-3 and Comparative Examples 1-3

[0056]

[0057] The above preparations were then divided into brown glass bottles, sealed, and placed in a 45°C constant temperature box with a relative humidity of 60%. The samples were taken at 0, 10, 20, and 30 days. The enzyme activity was determined according to the above method, and the activity retention rate R was calculated. t , the formula is as follows:

[0058]

[0059] Among them, R t (%) is the enzyme activity retention rate (percentage) after time t; A t is the enzyme activity measured at time t; A0 is the enzyme activity measured at the initial time (t=0). The results are shown in Table 2:

[0060] Table 2. Enzyme activity retention rate results of 45℃ accelerated aging experiment (%)

[0061] sample 0 days 10 days 20 days 30 days Example 1 100.0 88.5±2.7 75.3±2.3 62.1±1.9 Example 2 100.0 90.2±2.8 79.6±2.4 68.3±2.1 Example 3 100.0 92.7±2.8 83.5±2.5 73.9±2.2 Comparative Example 1 100.0 65.3±2.0 42.7±1.3 27.5±0.8 Comparative Example 2 100.0 61.8±1.9 38.2±1.2 23.1±0.7 Comparative Example 3 100.0 52.4±1.6 27.5±0.8 14.3±0.4

[0062] As shown in Tables 1 and 2, the initial cellulase activity (1520-1950 U / g) of the example group using the copolymer carrier was significantly higher than that of the comparative example group (810-1050 U / g). After 30 days of accelerated aging at 45°C, the enzyme activity retention rate (62.1-73.9%) of the example group was much higher than that of the comparative example group (14.3-27.5%). The copolymer carrier was synthesized by specific steps in the preparation example, and it has a special structure and properties. In the preparation process of agricultural biocomposite enzyme preparations, the copolymer carrier can provide a suitable microenvironment for the enzyme. On the one hand, it can adsorb enzyme molecules, reduce mutual collisions and aggregation between enzyme molecules, thereby reducing the probability of enzyme inactivation, maintaining the active conformation of the enzyme, allowing more enzyme molecules to play a catalytic role, and thus showing higher enzyme activity. On the other hand, the copolymer carrier may have a certain protective effect, which can block the direct effects of adverse external factors on the enzyme molecules and maintain the activity of the enzyme. Therefore, in the accelerated aging experiment, the enzyme activity retention rate is higher.

[0063] Comparative Example 1 uses hydroxyethyl acrylate to replace the copolymer carrier, Comparative Example 2 uses isoprene to replace the copolymer carrier, and Comparative Example 3 uses aluminum acetylacetonate to replace the copolymer carrier. Enzyme molecules are prone to aggregation and inactivation, resulting in reduced enzyme activity, and in the accelerated aging process, the enzyme activity decreases faster, and the enzyme activity retention rate is low. There may be certain interactions between the copolymer carrier and the enzyme, such as hydrogen bonds, van der Waals forces, etc. These interactions can stabilize the active conformation of the enzyme and prevent the enzyme from denaturing under conditions such as high temperatures. At the same time, the copolymer carrier can also buffer the effects of changes in the external environment on the enzyme. For example, in a 45°C accelerated aging experiment, the copolymer carrier can slow down the rate at which the enzyme activity decreases, improve the stability of the enzyme, and enable the enzyme to maintain a high activity over a long period of time. The comparative examples cannot effectively buffer the effects of changes in the external environment on the enzyme because the alternative substances do not form similar stable interactions with the enzyme. Under high temperature conditions, the enzyme molecules are more likely to undergo structural changes, resulting in a rapid decrease in enzyme activity, and the enzyme activity retention rate is low.

[0064] In summary, the copolymer carrier in the agricultural biocomposite enzyme preparation prepared by the present invention is a key factor in improving enzyme performance, enhancing enzyme activity, and stability. The rational selection and application of copolymer carriers is of great significance for the preparation of efficient and stable agricultural biocomposite enzyme preparations and for enhancing their application in agricultural production.

[0065] In summary, the copolymer carriers prepared in Preparation Examples 1-3 can significantly improve the enzyme activity and stability of the agricultural biocomplex enzyme preparations prepared in Examples 1-3.

[0066] Throughout the specification, reference to terms such as "Preparation," "Example," or "Examples" indicates that the specific features, structures, materials, or characteristics described in conjunction with that embodiment or preparation are included in at least one embodiment or preparation of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or preparation. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or preparations.

[0067] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. An agricultural biocomplex enzyme preparation, characterized in that: The following raw materials are included in parts by weight: 30-50 parts of complex enzyme, 5-8 parts of trehalose, 3-5 parts of glycerol, 15-20 parts of copolymer carrier, 5-8 parts of humic acid, 5-10 parts of bran, 3-5 parts of dipotassium hydrogen phosphate and 85-124 parts of water.

2. The agricultural biocomplex enzyme preparation according to claim 1, characterized in that: Based on the total weight of the complex enzyme, the enzyme components of the complex enzyme include: 8-12 parts of cellulase, 4-6 parts of lysozyme, 6-9 parts of transaminase, 4-6 parts of protease, 2-3 parts of oxidase and 2-3 parts of oxidoreductase.

3. The agricultural biocomplex enzyme preparation according to claim 1, characterized in that: The copolymer carrier comprises the following steps to prepare: S1. Under nitrogen protection, hydroxyethyl acrylate and isoprene were added to a reactor pre-charged with N-methylpyrrolidone, and azobisisobutyronitrile was added. The mixture was stirred at controlled temperature for 6-8 hours. After the reaction was completed, the mixture was cooled in an ice bath to precipitate, filtered, washed, and dried to obtain a copolymer powder. S2. Under nitrogen protection, add the copolymer powder to N-methylpyrrolidone, add propylene carbonate and aluminum acetylacetonate, heat in an oil bath, stir and react for 12-16 hours. After the reaction is completed, cool to 60-65°C, add 1% hydrochloric acid solution, add ice water dropwise to the reaction solution for precipitation, filter, wash and dry to obtain a copolymer carrier.

4. The agricultural biocomplex enzyme preparation according to claim 3, characterized in that: The mass ratio of hydroxyethyl acrylate, isoprene, N-methylpyrrolidone and azobisisobutyronitrile in step S1 is (83-85):(16-18):(300-320):(1-2).

5. The agricultural biocomplex enzyme preparation according to claim 3, characterized in that: In step S2, the mass ratio of the copolymer powder, N-methylpyrrolidone, propylene carbonate and aluminum acetylacetonate is (100-105):(300-320):(18-22):(15-16).

6. The agricultural biocomplex enzyme preparation according to claim 3, characterized in that: In step S1, the temperature is controlled to 70-80° C. and the stirring speed is 100-200 rpm.

7. The agricultural biocomplex enzyme preparation according to claim 3, characterized in that: In step S2, the temperature of the oil bath is raised to 110-120° C., and the stirring speed is 200-300 rpm.

8. A method for preparing the agricultural biocomplex enzyme preparation according to any one of claims 1 to 7, characterized in that: The following steps are involved: Trehalose, glycerol and water are added to a mixing tank according to weight, and stirred until dissolved. At room temperature, the complex enzyme is slowly added and stirred for 30-40 minutes. The copolymer carrier and dipotassium hydrogen phosphate are added and stirred for adsorption for 2-3 hours. The pH value is adjusted. Humic acid and bran are added in sequence, and high-speed shearing is performed for 10-20 minutes. The wet granules are formed by an extrusion granulator to obtain wet granules. The wet granules are spread on a dryer, dried for 50-60 minutes, and sieved to obtain an agricultural biological complex enzyme preparation.

9. The method for preparing an agricultural biocomplex enzyme preparation according to claim 8, characterized in that: The stirring speed is 100-200 rpm, the pH value is adjusted to 6.8±0.2, the high-speed shearing speed is 600-700 rpm, and the drying temperature is 40-50°C.

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