Targeted prebiotic plant extraction dietary powder for conditioning hyperlipidemia and preparation technology thereof

Abstract

The invention discloses targeted prebiotic plant extraction dietary powder for conditioning hyperlipidemia and a preparation process of the targeted prebiotic plant extraction dietary powder. In the invention, patent strains such as lactobacillus plantarum LP-12, bifidobacterium longum BL-08 and the like in the formula can regulate intestinal flora balance and inhibit activity of liver cholesterol synthesis key enzymes, so that generation of endogenous cholesterol is reduced from the source; natural monacolin K and phytosterol ester in the red yeast rice powder respectively inhibit cholesterol synthesis and competitively block exogenous cholesterol absorption to form a bidirectional regulation mechanism. Meanwhile, the oat beta-glucan forms an adhesive layer in the intestinal tract, so that part of lipid absorption is physically blocked; and prebiotics such as resistant dextrin and fructo-oligosaccharide provide nutrients for probiotics, promote secretion of short-chain fatty acids and further activate lipid metabolism related pathways. The components cooperate with one another to cover the whole chain of cholesterol synthesis, absorption and metabolism, so that the multi-dimensional lipid-lowering effect of'source inhibition-process blocking-metabolic regulation 'is realized.

Description

Technical Field

[0001] The present invention belongs to the technical field of extracted dietary powders, and in particular relates to a targeted prebiotic plant extract dietary powder for regulating hyperlipidemia and a preparation process thereof. Background Art

[0002] Plant-derived dietary powder is a nutritional supplement made by extracting active ingredients from various plants using advanced extraction technologies. It is widely used in the fields of healthy diets and functional foods. It typically uses herbs as its primary raw material. Through processes such as cleaning, drying, low-temperature extraction, concentration, and spray drying, the plant's protein, dietary fiber, vitamins, minerals, polyphenol antioxidants, and active ingredients are efficiently retained and concentrated into a powder form. Plant-derived dietary powder boasts high nutritional density, easy absorption, no additives, and low calories, making it suitable for those pursuing a healthy diet, weight control, nutritional supplements, and vegetarians. Its formula can be scientifically formulated to meet the needs of different groups, such as enhancing immunity, improving intestinal health, and regulating blood sugar and blood lipids. It also offers excellent solubility and palatability, allowing it to be consumed directly or added to beverages and baked goods. It is an ideal choice for modern people pursuing a convenient, nutritious, and healthy lifestyle.

[0003] However, in the existing technology, traditional plant-extracted dietary powder products lack targeted designs for blood lipid regulation, and it is difficult to simultaneously act on the entire chain of cholesterol synthesis, absorption and metabolism; the plant ingredient extraction process often destroys its activity due to high temperature or chemical treatment, resulting in low utilization of effective ingredients; probiotics are not encapsulated, and their survival rate in gastric acid is insufficient, making it difficult for them to colonize in the intestines and exert their effects; some products have a single form, require complex brewing when used, or are only suitable for a single scenario; and some products add chemical synthetic ingredients to enhance the effect, which poses a safety risk for long-term consumption. Summary of the Invention

[0004] The purpose of the present invention is to provide a targeted prebiotic plant extract dietary powder for regulating hyperlipidemia and a preparation process thereof in order to solve the above-mentioned problems.

[0005] The technical solution adopted by the present invention is as follows: a targeted prebiotic plant extract dietary powder for regulating hyperlipidemia, the dietary powder comprising:

[0006] Lactobacillus plantarum LP-12: 2 parts by weight;

[0007] Bifidobacterium longum BL-08: 2 parts by weight;

[0008] Lactobacillus rhamnosus: 1 part by weight;

[0009] Resistant dextrin: 16 parts by weight;

[0010] Fructooligosaccharides: 10 parts by weight;

[0011] Inulin: 9 parts by weight;

[0012] Red yeast rice powder: 12 parts by weight;

[0013] Phytosterol esters: 8 parts by weight;

[0014] Oat β-glucan: 10 parts by weight;

[0015] Hawthorn flavonoids: 5 parts by weight;

[0016] Celery seed extract: 5 parts by weight;

[0017] Maltodextrin DE18-80: 8 parts by weight;

[0018] Vitamin B complex: 1 part by weight;

[0019] Vitamin E: 1 part by weight;

[0020] Microcrystalline cellulose: 10 parts by weight.

[0021] In a preferred embodiment, a process for preparing a targeted prebiotic plant extract dietary powder for regulating hyperlipidemia comprises the following steps:

[0022] S1: Raw material quality screening and pretreatment; select plant raw materials such as red yeast rice powder, oat β-glucan, hawthorn flavonoids that meet the GB 24154 standard and test their active ingredient content; conduct activity testing on probiotic strains to ensure that the raw materials meet the formula requirements and provide a high-quality foundation for subsequent extraction and embedding;

[0023] S2: Extraction of plant active ingredients: Hawthorn, celery seeds and other raw materials are crushed to 80 mesh, and flavonoids are extracted using CO2 supercritical extraction technology. Oats are simultaneously subjected to β-glucan water extraction and concentrated to a solid content of 40%, retaining more than 98% of the active ingredients, providing highly active plant extracts for subsequent mixing;

[0024] S3: Probiotic encapsulation treatment: The three probiotics were fermented in MRS medium and then centrifuged to collect the cells. The cells were mixed with modified starch and sodium alginate in a ratio of 1:2:1 and then encapsulated into encapsulated bacterial powder using nano-scale microencapsulation technology to ensure that the probiotics remained active during subsequent mixing and storage.

[0025] S4: Pretreatment of prebiotics and carriers; passing resistant dextrin, oligofructose, and inulin through a 60-mesh sieve to remove agglomerated particles; passing microcrystalline cellulose through an 80-mesh sieve to improve powder fluidity and lay the foundation for subsequent uniform mixing;

[0026] S5: Vitamin premix; vitamin B complex, vitamin E and maltodextrin DE18-80 were mixed at a low speed in a three-dimensional mixer at a ratio of 1:5 for 10 minutes to form a premix to prevent the vitamins from agglomerating during the total mixing due to their small particle size;

[0027] S6: Multi-stage uniform mixing: first add microcrystalline cellulose as a base powder to the double cone mixer, then add the sieved prebiotics and plant extracts in sequence and mix for 20 minutes; then add the vitamin premix and mix for 15 minutes; finally add the embedded probiotic powder and mix at low speed for 30 minutes to ensure that all ingredients are evenly distributed and avoid inactivation of the probiotics due to excessive shearing;

[0028] S7: Low-temperature drying and shaping; the mixed material is dried in a fluidized bed to a moisture content of ≤3% to prevent moisture absorption and agglomeration while protecting the activity of probiotics and heat-sensitive ingredients;

[0029] S8: Quantitative packaging and sealing; use an automatic powder filling machine to package the powder into 4g bags, seal them with aluminum-plastic composite bags, and use a metal detector to detect foreign matter after packaging. The final product is easy to carry and store, ensuring the stability of the product during transportation and storage.

[0030] In a preferred embodiment, in step S1, first, for plant raw materials, red yeast rice powder, oat β-glucan, hawthorn flavonoids, celery seed extract and other ingredients are selected, and according to GB 24154 special dietary sports nutrition food standard, the monacolin K content in the red yeast rice powder is tested to be not less than 0.3%, the purity of oat β-glucan is not less than 85%, and the total flavonoids content in hawthorn flavonoids and celery seed extract is not less than 12% and 15%, respectively; for probiotic raw materials, Lactobacillus plantarum LP-12, Bifidobacterium longum BL-08, and Lactobacillus rhamnosus must pass activity testing to ensure that the number of viable bacteria per gram of bacterial powder is not less than 10 billion CFU.

[0031] In a preferred embodiment, in step S2, for raw materials such as hawthorn and celery seeds from which flavonoid components need to be extracted, they are first crushed into 80-mesh particles, and then a CO2 supercritical extraction device is used, with the extraction pressure set to 35 MPa, the extraction temperature to 45°C, and the extraction time to 2 hours. The high permeability and solubility of the supercritical CO2 fluid are used to efficiently extract the flavonoid active ingredients, and after the extraction is completed, a high-purity extract is obtained by vacuum separation.

[0032] In a preferred embodiment, in step S3, first, Lactobacillus plantarum LP-12, Bifidobacterium longum BL-08, and Lactobacillus rhamnosus are inoculated into MRS liquid culture medium, respectively, and fermented in a constant temperature incubator at 37°C for 48 hours. When the OD600 value of the bacterial liquid reaches 1.2 to 1.5, the bacteria are collected by a centrifuge; the collected bacteria are mixed with modified starch and sodium alginate in a mass ratio of 1:2:1 to form a bacterial suspension, and then nano-microcapsule encapsulation technology is used to perform encapsulation treatment through a spray drying equipment, the inlet temperature is set to 85°C, the outlet temperature is set to 40°C, and the spray pressure is controlled to 0.3 MPa, and finally the embedded bacterial powder is obtained.

[0033] In a preferred embodiment, in step S4, the three prebiotic raw materials of resistant dextrin, oligofructose and inulin in the formula are sieved through a 60-mesh sieve to remove agglomerated particles and large-particle impurities to ensure uniform particle size of the material; microcrystalline cellulose is used as a carrier raw material and sieved through an 80-mesh sieve to further refine the particles and reduce friction between the powders.

[0034] In a preferred embodiment, in step S5, the vitamin B complex, vitamin E and maltodextrin DE18-80 in the formula are added into a three-dimensional mixer in a mass ratio of 1:5, the mixer speed is set to 15 rpm, and the mixing time is 10 minutes; through the multi-directional movement of the three-dimensional mixer, the vitamins are evenly dispersed in the maltodextrin carrier to form a premix.

[0035] In a preferred embodiment, in step S6, microcrystalline cellulose is first added to the double-cone mixer as a base powder, and its good carrying capacity is used to provide a dispersion basis for other materials; then, the three prebiotics of resistant dextrin, oligofructose, and inulin after sieving, as well as plant active ingredients such as red yeast rice powder, phytosterol esters, oat β-glucan concentrate, hawthorn flavonoids, and celery seed extract are added in sequence, and the mixer speed is set to 20 rpm and the mixing time is 20 minutes to fully mix the plant ingredients and prebiotics; after the above materials are evenly mixed, the vitamin premix is ​​added, the speed is adjusted to 18 rpm, and the mixing time is 15 minutes to ensure that the vitamins are integrated with other materials; finally, the embedded probiotic powder is added, the speed is reduced to 10 rpm, and the mixing time is extended to 30 minutes.

[0036] In a preferred embodiment, in step S7, the mixed material is put into a fluidized bed dryer, the inlet air temperature is set to 35°C, and the material is dried by sufficient contact between the hot air and the material. The material temperature is monitored in real time during the drying process to ensure that it does not exceed 37°C; and the drying is stopped when the moisture content of the material is ≤3%.

[0037] In a preferred embodiment, in step S8, an automatic powder filling machine is used for subpackaging, the single filling volume is set to 4 grams / bag, and the filling accuracy is controlled within ±0.1 grams; the subpacked material is sealed in an aluminum-plastic composite bag, and the sealing is completed by a heat sealing device, and the heat sealing temperature is set to 120°C, the heat sealing pressure is set to 0.3 MPa, and the heat sealing time is set to 2 seconds to ensure that the packaging bag is tightly sealed to prevent oxygen and moisture from entering; after packaging is completed, each bag of product is inspected by a metal detector to eliminate unqualified products containing metal impurities, and finally a finished product that is easy to carry and store is formed. After verification by accelerated testing, the product viable bacteria retention rate is ≥90%, and the active ingredient loss rate is ≤5%, which meets the long-term storage requirements.

[0038] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0039] 1. In the present invention, the patented strains such as Lactobacillus plantarum LP-12 and Bifidobacterium longum BL-08 in the formula regulate the balance of intestinal flora, inhibit the activity of key enzymes in liver cholesterol synthesis, and reduce endogenous cholesterol production from the source; the natural monacolin K and plant sterol esters in red yeast rice flour respectively inhibit cholesterol synthesis and competitively block the absorption of exogenous cholesterol, forming a two-way regulatory mechanism. At the same time, oat β-glucan forms a sticky layer in the intestine, physically blocking the absorption of some lipids; prebiotics such as resistant dextrin and oligofructose provide nutrients for probiotics, promote the secretion of short-chain fatty acids, and further activate lipid metabolism-related pathways. These ingredients work together to cover the entire chain of cholesterol synthesis, absorption, and metabolism, achieving a multi-dimensional lipid-lowering effect from "source inhibition-process blocking-metabolic regulation."

[0040] 2. In the present invention, probiotics are processed by nano-microcapsule embedding technology to ensure that the strain has a high survival rate in the gastric acid environment and can successfully colonize the intestines to exert its effect; CO2 supercritical extraction and low-temperature drying processes retain the biological efficacy of the plant active ingredients to the greatest extent and avoid the inactivation of heat-sensitive substances. The independent 4-gram package design meets the daily dietary fiber supplementation requirement of the human body, and the 3-second instant dissolving technology is suitable for various scenarios such as sports and office work. The hot and cold dual-solubility characteristics improve the convenience of use. In addition, the product strictly follows special dietary standards, does not contain burdensome ingredients such as chemical additives and trans fatty acids, and is highly safe. Long-term consumption can help improve dyslipidemia caused by bad living habits or metabolic disorders. At the same time, by regulating the balance of intestinal flora, it indirectly improves the overall metabolic health level, providing a scientific and gentle conditioning option for people with hyperlipidemia. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 It is a schematic diagram of the process principle of the present invention. DETAILED DESCRIPTION

[0042] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0043] Example:

[0044] Reference Figure 1 ,

[0045] A targeted prebiotic plant extract dietary powder for regulating hyperlipidemia, comprising:

[0046] Lactobacillus plantarum LP-12 (patented lipid-lowering strain): 2 parts by weight;

[0047] Bifidobacterium longum BL-08 (DuPont patented strain): 2 parts by weight;

[0048] Lactobacillus rhamnosus (synergistically regulates lipid metabolism): 1 part by weight;

[0049] Resistant dextrin (SCFAs precursor, promotes lipolysis): 16 parts by weight;

[0050] Fructooligosaccharide (prebiotic enhancement, 3:1 golden ratio): 10 parts by weight;

[0051] Inulin (regulates purine metabolism and promotes uric acid excretion): 9 parts by weight;

[0052] Red yeast rice powder (natural monacolin K, inhibits cholesterol synthesis): 12 parts by weight;

[0053] Phytosterol esters (competitively inhibit cholesterol absorption): 8 parts by weight;

[0054] Oat β-glucan (forms intestinal mucus layer, blocking lipid absorption): 10 parts by weight;

[0055] Hawthorn flavonoids (improve coronary blood flow and regulate vascular elasticity): 5 parts by weight;

[0056] Celery seed extract (flavonoids lower lipids and inhibit inflammatory factors): 5 parts by weight;

[0057] Maltodextrin DE18-80 (medium-low GI sustained-release energy supply): 8 parts by weight;

[0058] Vitamin B complex (lipid metabolism coenzyme activation): 1 part by weight;

[0059] Vitamin E (antioxidant, protects vascular endothelium): 1 part by weight;

[0060] Microcrystalline cellulose (carrier, to ensure powder fluidity): 10 parts by weight.

[0061] A preparation process of a targeted prebiotic plant extract dietary powder for regulating hyperlipidemia comprises the following steps:

[0062] S1: Raw material quality screening and pretreatment. Select plant raw materials such as red yeast rice powder, oat β-glucan, and hawthorn flavonoids that meet the GB 24154 standard and test their active ingredient content (for example, the content of monacolin K in red yeast rice is ≥0.3%); perform activity testing on probiotic strains (Lactobacillus plantarum LP-12, Bifidobacterium longum BL-08, and Lactobacillus rhamnosus) (viable count ≥10 billion CFU / g) to ensure that the raw materials meet the formula requirements and provide a high-quality foundation for subsequent extraction and encapsulation.

[0063] S2: Extraction of plant active ingredients. Hawthorn and celery seeds were ground into 80 mesh, and flavonoids were extracted using supercritical CO2 extraction technology (extraction pressure 35MPa, temperature 45°C, time 2 hours). Oats were simultaneously subjected to β-glucan water extraction (solid-to-liquid ratio 1:15, extraction at 60°C for 3 hours) and concentrated to a solid content of 40%, retaining more than 98% of the active ingredients, providing highly active plant extracts for subsequent mixing.

[0064] S3: Probiotic Encapsulation Treatment. The three probiotics were fermented in MRS medium (37°C, 48 hours) and then centrifuged. The cells were mixed with modified starch and sodium alginate in a 1:2:1 ratio. Nanoscale microencapsulation technology (spray drying inlet temperature 85°C, outlet temperature 40°C) was used to produce an encapsulated bacterial powder (gastric acid survival rate ≥88%), ensuring that the probiotics remained active during subsequent mixing and storage.

[0065] S4: Pretreatment of prebiotics and carriers. Pass resistant dextrin, oligofructose, and inulin through a 60-mesh sieve to remove agglomerated particles; pass microcrystalline cellulose through an 80-mesh sieve to improve powder fluidity and lay the foundation for subsequent uniform mixing.

[0066] S5: Vitamin Premix: Mix B complex vitamins, vitamin E, and maltodextrin DE18-80 in a 1:5 ratio in a three-dimensional mixer at low speed (15 rpm) for 10 minutes to form a premix to prevent the vitamins from agglomerating during the final mixing due to their small particle size.

[0067] S6: Multi-stage uniform mixing. First, add microcrystalline cellulose as a base powder to the double cone mixer, then add the sifted prebiotics (resistant dextrin, oligofructose, inulin), plant extracts (red yeast rice powder, phytosterol esters, oat beta-glucan concentrate, hawthorn flavonoids, celery seed extract) in sequence and mix for 20 minutes; then add the vitamin premix and mix for 15 minutes; finally, add the embedded probiotic powder and mix at low speed (10 rpm) for 30 minutes to ensure that all ingredients are evenly distributed and avoid inactivation of the probiotics due to excessive shearing.

[0068] S7: Low-temperature drying and shaping. The mixed material is dried in a fluidized bed (inlet air temperature 35°C, material temperature ≤37°C) to a moisture content of ≤3%. This prevents moisture absorption and agglomeration, while protecting the activity of probiotics and heat-sensitive ingredients (such as B vitamins).

[0069] S8: Quantitative packaging and sealing. Use an automatic powder filling machine to pack the powder into 4g bags, seal them in aluminum-plastic composite bags (heat sealing temperature 120°C, pressure 0.3MPa), and use a metal detector to check for foreign matter after packaging. The final product is easy to carry and store, ensuring product stability during transportation and storage.

[0070] In the step S1, first, for plant raw materials, select ingredients such as red yeast rice powder, oat beta-glucan, hawthorn flavonoids, and celery seed extract. According to GB 24154 special dietary sports nutrition food standard, the monacolin K content in the red yeast rice powder needs to be not less than 0.3%, the purity of oat beta-glucan needs to be not less than 85%, and the total flavonoids content in hawthorn flavonoids and celery seed extract is not less than 12% and 15% respectively. For probiotic raw materials, plant lactobacillus LP-12, Bifidobacterium longum BL-08, and Lactobacillus rhamnosus need to pass activity detection to ensure that the number of viable bacteria in each gram of bacterial powder is not less than 10 billion CFU. After all raw materials are tested and qualified, the plant raw materials are removed and cleaned, and the probiotic powder is subjected to freeze-dried product morphology inspection to ensure that there is no moisture absorption and caking phenomenon, providing high-quality basic raw materials for subsequent extraction, embedding and other processes.

[0071] In step S2, for raw materials such as hawthorn and celery seeds that need to be extracted with flavonoid components, they are first crushed into 80 mesh particles, and then a CO2 supercritical extraction device is used, with the extraction pressure set to 35MPa, the extraction temperature to 45°C, and the extraction time to 2 hours. The high permeability and solubility of the supercritical CO2 fluid are used to efficiently extract the flavonoid active ingredients. After the extraction is completed, a high-purity extract is obtained by vacuum separation. For oat raw materials, β-glucan is extracted by water extraction. The specific process is as follows: oat flour and deionized water are mixed at a solid-liquid ratio of 1:15, and extracted in a constant temperature water bath at 60°C for 3 hours. After the extraction is completed, centrifugation (3000 rpm, 10 minutes) is performed to obtain the supernatant, which is then concentrated to a solid content of 40% by a vacuum concentrator. This process can retain more than 98% of the β-glucan activity.

[0072] In described step S3, first plant lactobacillus LP-12, Bifidobacterium longum BL-08, lactobacillus rhamnosus are inoculated into MRS liquid nutrient medium respectively, fermented 48 hours in 37 DEG C of constant temperature incubators, when bacterium liquid OD600 value reaches 1.2~1.5, by centrifuge (4000 rev / min, 15 minutes) collect thalline.The thalline collected is mixed with modified starch, sodium alginate in the mass ratio of 1:2:1, forms bacterial suspension, adopts nanometer microcapsule embedding technology subsequently, carries out embedding process by spray drying equipment, sets inlet air temperature 85 DEG C, outlet air temperature 40 DEG C, controls spray pressure 0.3MPa, finally obtains embedded bacteria powder.After testing, the embedded bacteria powder gastric acid survival rate of this process preparation is not less than 88%, can ensure that probiotics survive in gastric acid environment and arrive intestinal colonization.

[0073] In step S4, the three prebiotic ingredients in the formula—resistant dextrin, oligofructose, and inulin—are sieved through a 60-mesh sieve to remove agglomerated particles and large impurities, ensuring uniform particle size. Microcrystalline cellulose, used as a carrier material, is sieved through an 80-mesh sieve to further refine the particles and reduce friction between the powders. This treatment significantly improves the fluidity of the prebiotic and carrier materials, preventing stratification due to particle size differences during subsequent mixing and ensuring uniform mixing.

[0074] In the step S5, the vitamin B group, vitamin E and maltodextrin DE18-80 in the formula are put into a three-dimensional mixer in a mass ratio of 1:5, and the mixer speed is set to 15 rev / min and the mixing time is 10 minutes. Through the multi-directional motion of the three-dimensional mixer, the vitamins are evenly dispersed in the maltodextrin carrier to form a premix. This step can effectively avoid the floating or agglomeration phenomenon that occurs due to the low density and fine particles of the vitamins in the subsequent total mixing process, and ensure that the vitamins are evenly distributed in the final product.

[0075] In the step S6, microcrystalline cellulose is first added to the double cone mixer as a base powder, and its good carrying capacity is used to provide a dispersion basis for other materials; then the three prebiotics of resistant dextrin, oligofructose, and inulin after sieving are added in sequence, as well as plant active ingredients such as red yeast rice powder, phytosterol esters, oat beta-glucan concentrate, hawthorn flavonoids, and celery seed extract, and the mixer speed is set to 20 rpm and the mixing time is 20 minutes to fully mix the plant components with the prebiotics; after the above materials are evenly mixed, the vitamin premix is ​​added, the speed is adjusted to 18 rpm, and the mixing time is 15 minutes to ensure that the vitamins are fused with other materials; finally, the embedded probiotic powder is added, the speed is reduced to 10 rpm, and the mixing time is extended to 30 minutes to avoid high-speed shearing from damaging the probiotic activity. This multi-stage mixing process can make all the ingredients evenly distributed, and the mixing uniformity RSD is tested to be ≤3%.

[0076] In step S7, the mixed material is placed in a fluidized bed dryer with the inlet air temperature set at 35°C. Drying occurs through full contact between the hot air and the material. The material temperature is monitored in real time during the drying process to ensure it does not exceed 37°C. Drying is stopped when the material moisture content is ≤3%. This moisture content prevents the material from absorbing moisture and clumping, while also preventing the inactivation of probiotics or the decomposition of heat-sensitive components such as B vitamins due to overdrying. After drying, the material's fluidity is further improved, facilitating the packaging process.

[0077] In described step S8, use automatic powder filling machine to carry out subpackaging, set single filling amount to 4 grams / bag, and filling precision is controlled within ± 0.1 gram. The material after subpackaging is sealed in aluminum-plastic composite bag, and sealing is completed by heat sealing equipment. It is set that heat sealing temperature is 120 DEG C, heat sealing pressure is 0.3MPa, and heat sealing time is 2 seconds, ensures that packaging bag is tightly sealed to prevent oxygen and moisture from entering. After packaging is completed, each bag of product is tested by metal detector, and the defective products containing metal impurities are rejected, and finally formed into the finished product that is easy to carry, store, and through accelerated test (40 DEG C, 75% humidity, 30 days) verification, product viable count retention rate ≥90%, active ingredient loss rate ≤5%, meet long-term storage requirements.

[0078] From the above we can know:

[0079] In the present invention, the patented strains of Lactobacillus plantarum LP-12, Bifidobacterium longum BL-08, etc. in the formula regulate the balance of intestinal flora, inhibit the activity of key enzymes in liver cholesterol synthesis, and reduce endogenous cholesterol production from the source; the natural monacolin K and phytosterol esters in red yeast rice flour respectively inhibit cholesterol synthesis and competitively block the absorption of exogenous cholesterol, forming a two-way regulatory mechanism. At the same time, oat β-glucan forms a sticky layer in the intestine, physically blocking the absorption of some lipids; prebiotics such as resistant dextrin and oligofructose provide nutrients for probiotics, promote the secretion of short-chain fatty acids, and further activate lipid metabolism-related pathways. These ingredients work together to cover the entire chain of cholesterol synthesis, absorption, and metabolism, achieving a multi-dimensional lipid-lowering effect from "source inhibition-process blocking-metabolic regulation".

[0080] In the present invention, probiotics are processed by nano-microcapsule embedding technology to ensure that the strain has a high survival rate in the gastric acid environment and can successfully colonize the intestines to exert its effect; CO2 supercritical extraction and low-temperature drying processes retain the biological efficacy of the plant active ingredients to the greatest extent and avoid the inactivation of heat-sensitive substances. The independent 4-gram package design meets the daily dietary fiber supplementation requirements of the human body, and the 3-second instant dissolving technology is suitable for various scenarios such as exercise and office work. The hot and cold dual-solubility characteristics improve the convenience of use. In addition, the product strictly follows special dietary standards, does not contain burdensome ingredients such as chemical additives and trans fatty acids, and is highly safe. Long-term consumption can help improve dyslipidemia caused by bad living habits or metabolic disorders. At the same time, by regulating the balance of intestinal flora, it indirectly improves the overall metabolic health level, providing a scientific and gentle conditioning option for people with hyperlipidemia.

[0081] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprises" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device that includes a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further limitations, an element defined by the sentence "comprises a ..." does not exclude the presence of other identical elements in the process, method, article or device that includes the element.

[0082] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A targeted prebiotic plant extract dietary powder for regulating hyperlipidemia, characterized by: The dietary powder comprises: Lactobacillus plantarum LP-12: 2 parts by weight; Bifidobacterium longum BL-08: 2 parts by weight; Lactobacillus rhamnosus: 1 part by weight; Resistant dextrin: 16 parts by weight; Fructooligosaccharides: 10 parts by weight; Inulin: 9 parts by weight; Red yeast rice powder: 12 parts by weight; Phytosterol esters: 8 parts by weight; Oat β-glucan: 10 parts by weight; Hawthorn flavonoids: 5 parts by weight; Celery seed extract: 5 parts by weight; Maltodextrin DE18-80: 8 parts by weight; Vitamin B complex: 1 part by weight; Vitamin E: 1 part by weight; Microcrystalline cellulose: 10 parts by weight.

2. The preparation process of the targeted prebiotic plant extract dietary powder for regulating hyperlipidemia according to claim 1, characterized in that: The preparation process comprises the following steps: S1: Raw material quality screening and pretreatment; select plant raw materials such as red yeast rice powder, oat β-glucan, hawthorn flavonoids that meet the GB 24154 standard and test their active ingredient content; conduct activity testing on probiotic strains to ensure that the raw materials meet the formula requirements and provide a high-quality foundation for subsequent extraction and embedding; S2: Extraction of plant active ingredients: Hawthorn, celery seeds and other raw materials are crushed to 80 mesh, and flavonoids are extracted using CO2 supercritical extraction technology. Oats are simultaneously subjected to β-glucan water extraction and concentrated to a solid content of 40%, retaining more than 98% of the active ingredients, providing highly active plant extracts for subsequent mixing; S3: Probiotic encapsulation treatment: The three probiotics were fermented in MRS medium and then centrifuged to collect the cells. The cells were mixed with modified starch and sodium alginate in a ratio of 1:2:1 and then encapsulated into encapsulated bacterial powder using nano-scale microencapsulation technology to ensure that the probiotics remained active during subsequent mixing and storage. S4: Pretreatment of prebiotics and carriers; passing resistant dextrin, oligofructose, and inulin through a 60-mesh sieve to remove agglomerated particles; passing microcrystalline cellulose through an 80-mesh sieve to improve powder fluidity and lay the foundation for subsequent uniform mixing; S5: Vitamin premix; vitamin B complex, vitamin E and maltodextrin DE18-80 were mixed at a low speed in a three-dimensional mixer at a ratio of 1:5 for 10 minutes to form a premix to prevent the vitamins from agglomerating during the total mixing due to their small particle size; S6: Multi-stage uniform mixing: first add microcrystalline cellulose as a base powder to the double cone mixer, then add the sieved prebiotics and plant extracts in sequence and mix for 20 minutes; then add the vitamin premix and mix for 15 minutes; finally add the embedded probiotic powder and mix at low speed for 30 minutes to ensure that all ingredients are evenly distributed and avoid inactivation of the probiotics due to excessive shearing; S7: Low-temperature drying and shaping; the mixed material is dried in a fluidized bed to a moisture content of ≤3% to prevent moisture absorption and agglomeration while protecting the activity of probiotics and heat-sensitive ingredients; S8: Quantitative packaging and sealing; use an automatic powder filling machine to package the powder into 4g bags, seal them with aluminum-plastic composite bags, and use a metal detector to detect foreign matter after packaging. The final product is easy to carry and store, ensuring the stability of the product during transportation and storage.

3. The preparation process of the targeted prebiotic plant extract dietary powder for regulating hyperlipidemia according to claim 1, characterized in that: In step S1, first, for plant raw materials, red yeast rice powder, oat β-glucan, hawthorn flavonoids, celery seed extract and other ingredients are selected. According to the standard of GB 24154 for special dietary sports nutrition foods, the monacolin K content in the red yeast rice powder must be not less than 0.3%, the purity of oat β-glucan must be not less than 85%, and the total flavonoids content in hawthorn flavonoids and celery seed extract must be not less than 12% and 15%, respectively; for probiotic raw materials, Lactobacillus plantarum LP-12, Bifidobacterium longum BL-08, and Lactobacillus rhamnosus must pass activity testing to ensure that the number of viable bacteria in each gram of bacterial powder is not less than 10 billion CFU.

4. The preparation process of the targeted prebiotic plant extract dietary powder for regulating hyperlipidemia according to claim 1, characterized in that: In step S2, for raw materials such as hawthorn and celery seeds from which flavonoid components need to be extracted, they are first crushed into 80-mesh particles, and then a CO2 supercritical extraction device is used, with the extraction pressure set to 35 MPa, the extraction temperature set to 45°C, and the extraction time set to 2 hours. The flavonoid active ingredients are efficiently extracted through the high permeability and solubility of the supercritical CO2 fluid, and a high-purity extract is obtained by vacuum separation after the extraction is completed.

5. The process for preparing the targeted prebiotic plant extract dietary powder for regulating hyperlipidemia according to claim 1, wherein: In the step S3, first, Lactobacillus plantarum LP-12, Bifidobacterium longum BL-08, and Lactobacillus rhamnosus are inoculated into MRS liquid culture medium, respectively, and fermented in a constant temperature incubator at 37°C for 48 hours. When the OD600 value of the bacterial liquid reaches 1.2 to 1.5, the bacteria are collected by a centrifuge; the collected bacteria are mixed with modified starch and sodium alginate in a mass ratio of 1:2:1 to form a bacterial suspension, and then nano-scale microcapsule encapsulation technology is adopted to perform encapsulation treatment through a spray drying device, the inlet air temperature is set to 85°C, the outlet air temperature is set to 40°C, and the spray pressure is controlled to 0.3 MPa, so as to finally obtain embedded bacterial powder.

6. The process for preparing the targeted prebiotic plant extract dietary powder for regulating hyperlipidemia according to claim 1, wherein: In step S4, the three prebiotic raw materials of resistant dextrin, oligofructose and inulin in the formula are sieved through a 60-mesh sieve to remove agglomerated particles and large-particle impurities to ensure uniform particle size of the materials; microcrystalline cellulose is used as a carrier raw material and sieved through an 80-mesh sieve to further refine the particles and reduce friction between the powders.

7. The process for preparing the targeted prebiotic plant extract dietary powder for regulating hyperlipidemia according to claim 1, wherein: In step S5, the B vitamins, vitamin E, and maltodextrin DE18-80 in the formula are added to a three-dimensional mixer at a mass ratio of 1:5, the mixer speed is set to 15 rpm, and the mixing time is 10 minutes; the vitamins are evenly dispersed in the maltodextrin carrier through the multi-directional movement of the three-dimensional mixer to form a premix.

8. The process for preparing the targeted prebiotic plant extract dietary powder for regulating hyperlipidemia according to claim 1, wherein: In the step S6, first, microcrystalline cellulose is added to the double-cone mixer as a base powder, and its good carrying capacity is used to provide a dispersion basis for other materials; then, three prebiotics, namely, resistant dextrin, oligofructose, and inulin, which have been sifted, as well as plant active ingredients such as red yeast rice powder, phytosterol esters, oat β-glucan concentrate, hawthorn flavonoids, and celery seed extract are added in sequence, and the mixer speed is set to 20 rpm and the mixing time is 20 minutes to fully mix the plant ingredients and the prebiotics; after the above materials are evenly mixed, the vitamin premix is ​​added, the speed is adjusted to 18 rpm, and the mixing time is 15 minutes to ensure that the vitamins are integrated with other materials; finally, the embedded probiotic powder is added, the speed is reduced to 10 rpm, and the mixing time is extended to 30 minutes.

9. The process for preparing the targeted prebiotic plant extract dietary powder for regulating hyperlipidemia according to claim 1, wherein: In step S7, the mixed material is put into a fluidized bed dryer, and the inlet air temperature is set to 35° C. The material is dried by sufficient contact between the hot air and the material. The material temperature is monitored in real time during the drying process to ensure that it does not exceed 37° C. The drying process is stopped when the moisture content of the material is ≤3%.

10. The process for preparing the targeted prebiotic plant extract dietary powder for regulating hyperlipidemia according to claim 1, wherein: In step S8, an automatic powder filling machine is used for subpackaging, a single filling amount is set to 4 g / bag, and a filling accuracy is controlled within ±0.1 g; the subpacked material is sealed in an aluminum-plastic composite bag, and the bag is sealed by a heat sealing device, with the heat sealing temperature set to 120° C., the heat sealing pressure set to 0.3 MPa, and the heat sealing time set to 2 seconds to ensure that the packaging bag is tightly sealed to prevent oxygen and moisture from entering; After packaging is completed, each bag of product is tested with a metal detector to eliminate unqualified products containing metal impurities, and finally a finished product that is easy to carry and store is formed. After verification by accelerated tests, the product's live bacteria retention rate is ≥90%, and the active ingredient loss rate is ≤5%, which meets the long-term storage requirements.

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