Liquid extraction device and extraction method

Abstract

The invention provides a liquid extraction device and an extraction method. The liquid extraction device comprises a pH detector, a first steady flow valve, an extracting solution switching valve, an extracting solution outflow pipeline, a second steady flow valve, a third steady flow valve, an eluent inflow pipeline, an extraction column, an absorbance detection unit and a controller, the pH detector, the first steady flow valve and the extracting solution switching valve are all arranged on the extracting solution outflow pipeline, the second steady flow valve and the third steady flow valve are both arranged on the eluent inflow pipeline, the input switching end of the extracting column is communicated with the outlet end of the eluent inflow pipeline, the outlet end of the extracting column is communicated with the inlet end of the extracting solution outflow pipeline, and the outlet end of the extracting column is communicated with the outlet end of the eluent inflow pipeline. The extracting solution outflow pipeline is communicated with the absorbance detection unit, and the extracting solution switching valve, the pH detector, the first steady flow valve, the second steady flow valve, the third steady flow valve and the absorbance detection unit are all connected with the controller. By adopting the technical scheme provided by the invention, the continuous automatic extraction of the proteolytic enzyme and the autonomous steady-state regulation and control of the extraction process are realized.

Description

Technical Field

[0001] The present application relates to the field of liquid extraction technology, and in particular to a liquid extraction device and extraction method. Background Art

[0002] In the pharmaceutical industry, the extraction process is a critical step in drug production. Its efficiency and quality impact key quality attributes of the final product, such as purity, yield, and bioactivity. Most existing proteolytic enzyme extraction methods require manual testing of key process parameters within each extraction column, and manual adjustments and switching of extraction processes based on these test results.

[0003] However, discontinuous manual testing methods result in delayed data feedback, making it impossible to adjust and switch extraction processes to optimal process parameters in a timely manner. Manual operations are based on empirical judgment, which affects extraction process stability and wastes resources. Furthermore, manual sampling can easily cause contamination, affecting test accuracy and leading to quality issues in the final product. Summary of the Invention

[0004] In view of this, the embodiments of the present application provide a liquid extraction device and extraction method, which can monitor the pH value, flow rate and absorbance of the extract of each extraction column in real time online, and automatically complete buffer switching, start and stop control of target extraction liquid collection, and balance adjustment of inlet and outlet liquid flow based on the detection data, thereby realizing continuous automated extraction of protein hydrolytic enzymes and autonomous steady-state regulation of the extraction process, ensuring the acquisition of protein hydrolytic enzymes with stable quality, improving the purification efficiency of protein hydrolytic enzymes, shortening detection time, reducing manual intervention, and reducing manual operation costs.

[0005] This application mainly includes the following aspects: In a first aspect, an embodiment of the present application provides a liquid extraction device, comprising: a pH detector, a first steady flow valve, an extraction liquid switching valve, an extraction liquid outflow pipeline, a second steady flow valve, a third steady flow valve, an eluent inflow pipeline, an extraction column, an absorbance detection unit, and a controller; The pH detector, the first steady flow valve and the extraction liquid switching valve are all arranged on the extraction liquid outflow pipeline, the second steady flow valve and the third steady flow valve are both arranged on the eluent inflow pipeline, the input switching end of the extraction column is connected to the outlet end of the eluent inflow pipeline, the outlet end of the extraction column is connected to the inlet end of the extraction liquid outflow pipeline, the extraction liquid outflow pipeline is connected to the absorbance detection unit, and the extraction liquid switching valve, the pH detector, the first steady flow valve, the second steady flow valve, the third steady flow valve and the absorbance detection unit are all connected to the controller.

[0006] Furthermore, the eluent inflow pipeline includes: a first buffer solution pipeline and a second buffer solution pipeline; the extraction liquid outflow pipeline includes: a first outflow pipeline; the extraction device also includes: a first flow meter, a second flow meter and a third flow meter; the input switching end of the extraction column includes: a first inlet end and a second inlet end; The first steady flow valve, the first flow meter and the extraction liquid switching valve are arranged on the first outflow pipeline, the second steady flow valve and the second flow meter are both arranged on the first buffer solution pipeline, the third steady flow valve and the third flow meter are arranged on the second buffer solution pipeline, the outlet end of the first buffer solution pipeline is connected to the first inlet end of the extraction column, the outlet end of the second buffer solution pipeline is connected to the second inlet end of the extraction column, the outlet end of the extraction column is connected to the inlet end of the first outflow pipeline, and the first flow meter, the second flow meter and the third flow meter are all connected to the controller.

[0007] Furthermore, the extraction device further comprises: a first one-way valve; the extraction liquid outflow pipeline comprises: a second outflow pipeline and a third outflow pipeline; the absorbance detection unit comprises: a detection pipeline, an inflow switching valve, a filter, a peristaltic pump, a debubbler, an ultraviolet detector and an outflow switching valve; A first one-way valve is arranged on the third outflow pipeline, and the inflow switching valve, the filter, the peristaltic pump, the debubbler, the ultraviolet detector and the outflow switching valve are all arranged on the detection pipeline. The inlet end of the second outflow pipeline is connected to the outlet end of the first flowmeter, the outlet end of the second outflow pipeline is connected to the input switching end of the inflow switching valve, the output switching end of the outflow switching valve is connected to the inlet end of the third outflow pipeline, and the outlet end of the third outflow pipeline is connected to the inlet end of the extraction liquid switching valve. The first one-way valve, the inflow switching valve, the filter, the peristaltic pump, the debubbler, the ultraviolet detector and the outflow switching valve are all connected to the controller.

[0008] Furthermore, the liquid extraction device further comprises: a collection pipeline and a waste liquid pipeline; The first outlet end of the extraction liquid switching valve is communicated with the inlet end of the waste liquid pipeline, and the second outlet end of the extraction liquid switching valve is communicated with the inlet end of the collection pipeline.

[0009] Furthermore, the eluent inflow pipeline further comprises: a first cleaning liquid inflow pipeline; the liquid extraction device further comprises: a first cleaning switching valve; The first cleaning switching valve is arranged on the first cleaning liquid inflow pipeline, the inlet end of the first cleaning switching valve is connected to the inlet end of the first cleaning liquid inflow pipeline, the first outlet end of the first cleaning switching valve is connected to the inlet end of the second flow stabilizing valve, the second outlet end of the first cleaning switching valve is connected to the inlet end of the third flow stabilizing valve, and the first cleaning switching valve is connected to the controller.

[0010] Furthermore, the eluent inflow pipeline further comprises: a second cleaning liquid inflow pipeline; the liquid extraction device further comprises: a second cleaning switching valve, a third cleaning liquid inflow pipeline, and a second one-way valve; the input switching end of the extraction column comprises: a third inlet end; The second cleaning switching valve is arranged on the second cleaning liquid inflow pipeline, and the second one-way valve is arranged on the third cleaning liquid inflow pipeline. The inlet end of the second cleaning switching valve is connected with the inlet end of the second cleaning liquid inflow pipeline, the first outlet end of the second cleaning liquid inflow pipeline is connected with the third inlet end of the extraction column, the second outlet end of the second cleaning liquid inflow pipeline is connected with the inlet end of the third cleaning liquid inflow pipeline, and the outlet end of the third cleaning liquid inflow pipeline is connected with the outlet end of the inflow switching valve; the second cleaning switching valve and the second one-way valve are both connected to the controller.

[0011] In a second aspect, an embodiment of the present application further provides a liquid extraction method, which is applied to an extraction device and includes: Obtaining the pH value of a first extract produced by the extraction column through a pH detector; wherein the first extract is produced by adsorbing the initial liquid in the extraction column; After the initial liquid in the extraction column is adsorbed for a first preset time, the first steady flow valve is controlled to open and the extraction liquid switching valve is controlled to be connected, so that the liquid that does not meet the collection condition flows out of the extraction column at a first set flow rate, and if the pH value is greater than a preset pH threshold, the second steady flow valve is controlled to open, so that the first buffer solution flows into the extraction column at a second set flow rate, and the extraction column produces a second extract; After the first buffer solution flows into the extraction column for a second preset time, obtaining a first sample liquid from the second extraction liquid by the absorbance detection unit, and obtaining a first absorbance of the first sample liquid; If the first absorbance is less than the first absorbance threshold, the third steady flow valve is controlled to open and the second steady flow valve is controlled to close, so that the second buffer solution flows into the extraction column at a third set flow rate, and the extraction column produces a third extract; obtaining a second sample liquid from the third extract liquid through an absorbance detection unit, and obtaining a second absorbance of the second sample liquid; If the second absorbance is less than the second absorbance threshold, the extraction liquid switching valve is controlled to switch the channel to obtain the target extraction liquid.

[0012] Furthermore, after the first buffer solution flows into the extraction column for a second preset time, obtaining a first sample liquid from the second extraction liquid by an absorbance detection unit, and obtaining a first absorbance of the first sample liquid, includes: After the first buffer solution flows into the extraction column for a second preset time, connecting the input switching end of the inflow switching valve that meets the connection condition with the outlet end of the inflow switching valve to allow the second extraction solution to flow into the detection pipeline; After the inflow switching valve is connected, the filter, the peristaltic pump and the debubbler are controlled to be opened, so that the second extract liquid is free of impurities and bubbles to obtain the first sample liquid, and the first sample liquid is made to flow to the ultraviolet detector at a fourth set flow rate; A first absorbance of the first sample liquid is obtained by an ultraviolet detector.

[0013] Furthermore, the extraction method further comprises: If the second absorbance is less than the third absorbance threshold, the extraction liquid switching valve is controlled to switch the channel, and the first cleaning switching valve, the second cleaning switching valve, the second one-way valve and the first steady flow valve are controlled to open, so that the first cleaning liquid and the second cleaning liquid clean the extraction device, and the liquid after cleaning the extraction device flows out from the waste liquid pipeline.

[0014] In a third aspect, an embodiment of the present application further provides a controller, comprising: A pH acquisition module, which acquires the pH value of a first extract produced by the extraction column through a pH detector; wherein the first extract is produced by adsorbing an initial liquid in the extraction column; The first buffer solution flows into the module. After the initial liquid in the extraction column is adsorbed for a first preset time, the first steady flow valve is controlled to open and the extraction liquid switching valve is controlled to be connected, so that the liquid that does not meet the collection conditions flows out of the extraction column at a first set flow rate. If the pH value is greater than a preset pH threshold, the first steady flow valve is controlled to open, so that the first buffer solution flows into the extraction column at a second set flow rate, and the extraction column produces a second extract; a first absorbance acquisition module, which acquires a first sample liquid from the second extracting liquid through an absorbance detection unit after the first buffer solution flows into the extraction column for a second preset time, and acquires a first absorbance of the first sample liquid; The second buffer solution flows into the module. If the second absorbance is less than the first absorbance threshold, the third steady flow valve is controlled to open and the first steady flow valve is controlled to close, so that the second buffer solution flows into the extraction column at a third set flow rate, and the extraction column produces a third extract; a second absorbance acquisition module, which acquires a second sample liquid from the third extract through an absorbance detection unit and acquires a second absorbance of the second sample liquid; The collection module controls the extraction liquid switching valve to switch the channel to obtain the target extraction liquid if the second absorbance is less than the second absorbance threshold.

[0015] The embodiments of the present application provide a liquid extraction device and extraction method. First, the pH value of a first extraction liquid is detected. If the pH value is greater than a preset pH threshold, a first buffer solution is injected to generate a second extraction liquid. A first sample liquid is obtained from the second extraction liquid, and the absorbance of the first sample liquid is detected to obtain a first absorbance. Then, if the first absorbance is less than the first absorbance threshold, the second buffer solution is switched to be injected to generate a third extraction liquid. After purification, the absorbance is detected. A second sample liquid is obtained from the third extraction liquid, and the absorbance of the second sample liquid is detected to obtain a second absorbance. Finally, if the second absorbance is less than the second absorbance threshold, the target extraction liquid is obtained by controlling the extraction liquid switching valve.

[0016] In this way, the present application can monitor the pH value, flow rate and absorbance of the extract of each extraction column online in real time, and automatically complete buffer switching, start and stop control of target extraction liquid collection, and balance adjustment of inlet and outlet liquid flow based on the detection data, thereby realizing continuous automated extraction of protein hydrolytic enzymes and autonomous steady-state regulation of the extraction process, ensuring the acquisition of protein hydrolytic enzymes with stable quality, improving the purification efficiency of protein hydrolytic enzymes, shortening detection time, reducing manual intervention, and reducing manual operation costs.

[0017] In order to make the above-mentioned objects, features and advantages of the present application more obvious and easy to understand, preferred embodiments are given below and described in detail with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following is a brief introduction to the drawings required for use in the embodiments. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without creative work.

[0019] Figure 1 A schematic structural diagram of a liquid extraction device provided in an embodiment of the present application is shown; Figure 2 A flow chart of a liquid extraction method provided in an embodiment of the present application is shown; Figure 3 It shows the real-time online monitoring dynamic diagram of pH value during the whole process of lumbrokinase extraction; Figure 4 One of the online real-time monitoring dynamic trend graphs of the flow rate flowing into the extraction column and the flow rate flowing out of the extraction column is shown; Figure 5 The real-time online monitoring dynamic trend diagram of absorbance during the whole process of lumbrokinase extraction is shown; Figure 6 The second dynamic trend diagram of the online real-time monitoring of the flow rate flowing into the extraction column and the flow rate flowing out of the extraction column is shown; Figure 7 A schematic diagram showing the comparison of control time between online detection and manual offline detection is shown; Figure 8 A schematic diagram showing the comparison of lumbrokinase quality between online detection and manual detection is shown; Figure 9 A controller provided in an embodiment of the present application is shown. DETAILED DESCRIPTION

[0020] In order to make the purpose, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. It should be understood that the drawings in the present application only serve the purpose of illustration and description and are not used to limit the scope of protection of the present application. In addition, it should be understood that the schematic drawings are not drawn to scale. The flowcharts used in this application illustrate the operations implemented according to some embodiments of the present application. It should be understood that the operations of the flowcharts can be implemented out of sequence, and steps without logical context can be reversed or implemented simultaneously. In addition, those skilled in the art, under the guidance of the contents of this application, can add one or more other operations to the flowchart, or remove one or more operations from the flowchart.

[0021] In addition, the described embodiments are only a part of the embodiments of the present application, rather than all of the embodiments. The components of the embodiments of the present application generally described and shown in the drawings here can be arranged and designed in various configurations. Therefore, the following detailed description of the embodiments of the present application provided in the drawings is not intended to limit the scope of the claimed application, but merely represents selected embodiments of the present application. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present application.

[0022] The following methods, devices, electronic devices or computer-readable storage media of the embodiments of the present application can be applied to any scenario requiring liquid extraction. The embodiments of the present application are not limited to specific application scenarios. Any scheme using the methods and devices provided by the embodiments of the present application is within the scope of protection of this application.

[0023] It is worth noting that in the pharmaceutical industry, the extraction process is a key link in the drug production process, and its efficiency and quality affect the key quality attributes of the final product, such as purity, yield and biological activity. Most of the existing proteolytic enzyme extraction methods require manual detection of the key process parameters of the substances in each extraction column, and manual adjustment and switching of the extraction process one by one according to the test results. However, the non-continuous manual detection method causes a delay in data feedback, resulting in the inability to adjust and switch the extraction process in time at the optimal process parameters. Manual operation is based on experience and judgment, which affects the stability of the extraction process and causes waste of resources. In addition, manual sampling operations are prone to contamination, affecting the accuracy of detection and causing quality problems in the final product.

[0024] In response to the above problems, the embodiments of the present application propose a liquid extraction device and extraction method. The present application can monitor the pH value, flow rate and absorbance of the extract of each extraction column in real time online, and automatically complete buffer switching, start and stop control of target extraction liquid collection, and balance adjustment of inlet and outlet liquid flow based on the detection data, thereby realizing continuous automated extraction of protein hydrolytic enzymes and autonomous steady-state regulation of the extraction process, ensuring the acquisition of protein hydrolytic enzymes with stable quality, improving the purification efficiency of protein hydrolytic enzymes, shortening detection time, reducing manual intervention, and reducing manual operation costs.

[0025] To facilitate understanding of the present application, the technical solutions provided in the present application are described in detail below in conjunction with specific embodiments.

[0026] See also Figure 1 , Figure 1 This is a schematic diagram of a liquid extraction device provided in an embodiment of the present application.

[0027] In the embodiments of the present application, the extracted liquid can be a proteolytic enzyme or other liquids, which are not limited here. The present application uses lumbrokinase, a proteolytic enzyme, as an example to illustrate the liquid extraction device and extraction method provided by the present application.

[0028] In the current proteolytic enzyme extraction process, key process parameters (such as UV absorbance, pH, and flow rate) and quality control indicators require manual testing and adjustment, resulting in a lag in process response. This delay in manual intervention is amplified with the increase in the number of extraction columns and the extension of the testing interval, causing process parameter deviations, which in turn affects the yield of the proteolytic enzyme and may lead to an increase in impurities and a decrease in dry powder specific activity. This lag in manual operation is primarily manifested in static adsorption switching, buffer equilibration, buffer elution, peak collection, and flow control. This lag causes process fluctuations, unstable potency transfer and loss rates, and inconsistent absorbance and potency trends. Furthermore, manual sampling can easily introduce contamination, affecting detection accuracy. Lag in operation also leads to buffer waste, increasing the burden on downstream processes, and ultimately compromising the stability of the extraction process and product quality.

[0029] like Figure 1 As shown in the figure, the liquid extraction device provided in the embodiment of the present application includes: a pH detector 10, a first steady flow valve 20, an extraction liquid switching valve 30, an extraction liquid outflow pipeline 40, a second steady flow valve 50, a third steady flow valve 60, an eluent inflow pipeline 70, an extraction column 80, an absorbance detection unit 90 and a controller 110.

[0030] Among them, the pH detector 10, the first steady flow valve 20 and the extract switching valve 30 are all arranged on the extract outflow pipeline 40, the second steady flow valve 50 and the third steady flow valve 60 are both arranged on the eluent inflow pipeline 70, the first steady flow valve 20 and the extract switching valve 30 are both arranged on the extract outflow pipeline 40, the input switching end of the extraction column 80 is connected to the outlet end of the eluent inflow pipeline 70, the outlet end of the extraction column 80 is connected to the inlet end of the extract outflow pipeline 40, the extract outflow pipeline 40 is connected to the absorbance detection unit 90, the extract switching valve 30, the pH detector 10, the first steady flow valve 20, the second steady flow valve 50, the third steady flow valve 60 and the absorbance detection unit 90 are all connected to the controller 110.

[0031] Here, the pH detector 10 is used to monitor the pH value of the extract; the first steady flow valve 20 is used to control the flow rate of the extract out of the extraction column 80; the extract switching valve 30 is used to switch the outflow path of the extract; the second steady flow valve 50 is used to control the flow rate of the first buffer solution when it flows into the extraction column 80; the third steady flow valve 60 is used to control the flow rate of the second buffer solution when it flows into the extraction column 80; the absorbance detection unit 90 is used to detect the absorbance of the extract; the controller 110 is used to generate a control signal based on the acquired detection data within a preset optimization time, control the actuator, stabilize the key process parameters within the ideal set range, and record and save the detection data, so as to facilitate the judgment of whether the extraction process is abnormal and whether it is stable based on the continuous real-time detection data, discover potential process optimization space, and provide data support for continuous improvement. Among them, the actuator includes the first steady flow valve 20, the second steady flow valve 50, and the third steady flow valve 60.

[0032] Specifically, controller 110 incorporates a multi-layered neural network (e.g., a long-short-term memory network for processing flow time series data, a convolutional neural network for extracting pH variation characteristics) and a reinforcement learning model (e.g., a reward-based optimization control strategy). This establishes a nonlinear mapping relationship between flow rate, pH value, absorbance (A value), and control variables. Prior to extraction, the model is pre-trained using historical extraction data (including parameter ranges corresponding to qualified products and various operating conditions) to achieve convergence. This model is then fine-tuned online using real-time data, enabling accurate prediction of parameter trends and intelligent decision-making regarding control actions. Control variables include the valve opening of the flow-stabilizing valve, liquid inflow, equipment operating parameters, and pipeline cleaning time.

[0033] The device connected to the controller 110 can communicate via wired (such as industrial Ethernet, RS485, etc.) or wireless (such as Bluetooth, Wi-Fi, 5G private network, etc.) communication methods and transmit to the controller 110 at a predetermined frequency.

[0034] Furthermore, the eluent inflow line 70 includes a first buffer line 71 and a second buffer line 72. The extraction liquid outflow line 40 includes a first outflow line 41. The extraction device also includes a first flowmeter 120, a second flowmeter 130, and a third flowmeter 140. The input switching end of the extraction column 80 includes a first inlet end and a second inlet end.

[0035] Among them, the first steady flow valve 20, the first flow meter 120 and the extraction liquid switching valve 30 are arranged on the first outflow pipeline 41, the second steady flow valve 50 and the second flow meter 130 are both arranged on the first buffer solution pipeline 71, the third steady flow valve 60 and the third flow meter 140 are arranged on the second buffer solution pipeline 72, the outlet end of the first buffer solution pipeline 71 is connected to the first inlet end of the extraction column 80, the outlet end of the second buffer solution pipeline 72 is connected to the second inlet end of the extraction column 80, the outlet end of the extraction column 80 is connected to the inlet end of the first outflow pipeline 41, and the first flow meter 120, the second flow meter 130 and the third flow meter 140 are all connected to the controller 110.

[0036] Here, the first flowmeter 120 is used to monitor the flow rate of the extract out of the extraction column 80; the second flowmeter 130 is used to monitor the flow rate of the first buffer solution flowing into the extraction column 80; and the third flowmeter 140 is used to monitor the flow rate of the second buffer solution flowing into the extraction column 80.

[0037] Furthermore, the extraction device also includes: a first one-way valve 150; the extraction liquid outflow pipeline 40 includes: a second outflow pipeline 42 and a third outflow pipeline 43; the absorbance detection unit 90 includes: a detection pipeline 91, an inflow switching valve 92, a filter 93, a peristaltic pump 94, a debubbler 95, an ultraviolet detector 96 and an outflow switching valve 97.

[0038] Here, the first one-way valve 150 is arranged on the third outflow pipe 43, the inflow switching valve 92, the filter 93, the peristaltic pump 94, the debubbler 95, the ultraviolet detector 96 and the outflow switching valve 97 are all arranged on the detection pipe 91, the inlet end of the second outflow pipe 42 is connected to the outlet end of the first flowmeter 120, the outlet end of the second outflow pipe 42 is connected to the input switching end of the inflow switching valve 92, the output switching end of the outflow switching valve 97 is connected to the inlet end of the third outflow pipe 43, the outlet end of the third outflow pipe 43 is connected to the inlet end of the extraction liquid switching valve 30, the first one-way valve 150, the inflow switching valve 92, the filter 93, the peristaltic pump 94, the debubbler 95, the ultraviolet detector 96 and the outflow switching valve 97 are connected to the controller 110.

[0039] Here, the first one-way valve 150 is used to prevent the extract in the first outflow pipe 41 from flowing back into the UV detector unit 90; the UV detector 96 is used to detect the absorbance of the sample liquid; the inflow switching valve 92 is used to switch the extract flowing into the detection pipe 91; the filter 93 is used to filter fine solid impurities in the extract to prevent impurities from settling when flowing through the 0.002 μm micro-circulation cell of the UV detector 96, thereby avoiding detection signal distortion or detection function failure due to light path blockage. Solid impurities include: mucus, fine sediment, miscellaneous proteins, and detached cellulose; the peristaltic pump 94 is used to provide conveying power to input the extract into the UV detector 96, maintaining a stable flow rate of the extract entering the UV detector 96 to prevent sedimentation; the debubbler 95 is used to eliminate bubbles mixed in the extract to prevent bubbles from interfering with normal detection of the UV light path; and the outflow switching valve 97 is used to flow the tested sample liquid into the corresponding third outflow pipe 43.

[0040] It should be noted that during the proteolytic enzyme extraction process, when the absorbance detection unit 90 is configured with only single-channel detection capability, the inflow switching valve 92 is connected to the outlet of a second outflow conduit 42 via a single input switching end, and the outflow switching valve 97 is connected to the inlet of a third outflow conduit 43 via a single output switching end, thereby enabling detection of the extract from a single extraction column 80. When the absorbance detection unit 90 is configured with multi-channel detection capability, the inflow switching valve 92 and the outflow switching valve 97 adopt a multi-port structure, with each independent input switching end of the inflow switching valve 92 connected to the corresponding outlet of the second outflow conduit 42, and each independent output switching end of the outflow switching valve 97 connected to the corresponding inlet of the third outflow conduit 43, thereby enabling uniform detection of the outflows from multiple extraction columns 80 and enabling switching control of each channel.

[0041] Furthermore, the liquid extraction device further comprises: a collection pipeline 160 and a waste liquid pipeline 170; Here, the first outlet end of the extracting liquid switching valve 30 is communicated with the inlet end of the waste liquid pipeline 170 , and the second outlet end of the extracting liquid switching valve 30 is communicated with the inlet end of the collecting pipeline 160 .

[0042] Furthermore, the eluent inflow pipeline 70 also includes: a first cleaning liquid inflow pipeline 73; the liquid extraction device also includes: a first cleaning switching valve 180; the input switching end of the extraction column 80 includes: a third inlet end, and the first cleaning switching valve 180 is connected to the controller 110.

[0043] Here, the first cleaning switching valve 180 is arranged on the first cleaning liquid inflow pipeline 73, the inlet end of the first cleaning switching valve 180 is connected to the inlet end of the first cleaning liquid inflow pipeline 73, the first outlet end of the first cleaning switching valve 180 is connected to the inlet end of the second steady flow valve 50, the second outlet end of the first cleaning switching valve 180 is connected to the inlet end of the third steady flow valve 60, and the first cleaning switching valve 180 is connected to the controller 110.

[0044] Here, the first cleaning switching valve 180 is used to allow the first cleaning solution to flow into the first buffer solution pipeline 71 , the second buffer solution pipeline 72 and the extraction solution outflow pipeline 40 .

[0045] Furthermore, the eluent inflow pipeline 70 also includes: a second cleaning liquid inflow pipeline 74; the liquid extraction device also includes: a second cleaning switching valve 190, a third cleaning liquid inflow pipeline 200 and a second one-way valve 210; the input switching end of the extraction column 80 includes: a third inlet end.

[0046] Here, the second cleaning switching valve 190 is arranged on the second cleaning liquid inflow pipeline 74, and the second one-way valve 210 is arranged on the third cleaning liquid inflow pipeline 200. The inlet end of the second cleaning switching valve 190 is connected to the inlet end of the second cleaning liquid inflow pipeline 74, the first outlet end of the second cleaning liquid inflow pipeline 74 is connected to the third inlet end of the extraction column 80, the second outlet end of the second cleaning liquid inflow pipeline 74 is connected to the inlet end of the third cleaning liquid inflow pipeline 200, and the outlet end of the third cleaning liquid inflow pipeline 200 is connected to the outlet end of the inflow switching valve 92; the second cleaning switching valve 190 and the second one-way valve 210 are both connected to the controller 110.

[0047] Here, the second cleaning switching valve 190 is used to flow the second cleaning liquid into the extraction column 80 and the first outflow pipe 41; the second one-way valve 210 is used to flow the second cleaning liquid into the ultraviolet detection unit.

[0048] See also Figure 2 , Figure 2 This is a flow chart of a liquid extraction method provided in an embodiment of the present application.

[0049] like Figure 2 As shown in , the liquid extraction method provided in the embodiment of the present application includes the following steps: In step S101 , the pH value of the first extraction solution generated by the extraction column 80 is obtained by using the pH detector 10 .

[0050] Here, the first extract is produced by adsorbing the initial liquid in the extraction column 80. The initial liquid is the liquid to be purified. As an example, the initial liquid can be earthworm slurry obtained by clarifying a standard volume of earthworm slurry. The standard volume can be 60 kg or other volumes, which are not limited here. The extraction column 80 can be made of organic glass or other materials, which are not limited here; the size of the extraction column 80 can be 0.6 m in inner diameter and 1.3 m in height, which can also be other sizes, which are not limited here.

[0051] Specifically, the initial liquid is added to a plurality of extraction columns 80, and the adsorption material pre-filled in the extraction column 80 gradually adsorbs the proteolytic enzyme in the initial liquid until it reaches a saturated state. During the adsorption process, the pH value of the liquid in the extraction column 80 gradually increases. As an example, if lumbrokinase is to be extracted, the extraction column 80 is filled with anti-fibrinolytic anion exchange cellulose, which gradually adsorbs the lumbrokinase in the earthworm slurry to generate a first extract. The pH value of the first extract is continuously obtained by the pH detector 10. As an example, the real-time online dynamic monitoring diagram of the pH value of the entire enzyme extraction process is as follows: Figure 3 shown.

[0052] In step S102, after the initial liquid in the extraction column 80 is adsorbed for a first preset time, the first steady flow valve 20 is controlled to open and the extraction liquid switching valve 30 is controlled to be connected, so that the liquid that does not meet the collection conditions flows out of the extraction column 80 according to the first set flow rate, and if the pH value is greater than the preset pH threshold, the first steady flow valve 20 is controlled to open, so that the first buffer solution flows into the extraction column 80 according to the second set flow rate, and the extraction column 80 produces a second extraction liquid.

[0053] Here, as an example, the first preset time can be set to three hours, and the preset pH threshold can be set to 8. The first preset time and the preset pH threshold can also be set to other values ​​based on other actual experience or working conditions, and there is no limitation here. The liquid that does not meet the collection conditions is the extract whose concentration does not meet the purification standard. The first set flow rate and the second set flow rate should be consistent, that is, the flow rate of the first buffer solution flowing into the extraction column 80 and the flow rate of the second extract flowing out of the extraction column 80 remain basically consistent, wherein the set flow rate can be a specific value or a range, and there is no limitation here. As an example, the dynamic trend diagram of the flow rate of the first buffer solution flowing into the extraction column 80 and the flow rate of the second extract flowing out of the extraction column 80 is shown as follows. Figure 4 shown.

[0054] In this embodiment of the present application, when the flow deviation exceeds the allowable range due to real-time fluctuations in pipeline pressure, the opening of the first flow stabilizing valve 20 or the second flow stabilizing valve 50 is automatically adjusted to maintain the flow within the set range. As an example, when the flow deviation exceeds the allowable range, the opening of the first flow stabilizing valve 20 is adjusted by 10%.

[0055] Specifically, in this step, after the initial liquid in the extraction column 80 has been adsorbed for a first preset time, the first steady flow valve 20 is controlled to open according to the first set valve opening, and the first flowmeter 120 is turned on, allowing the first extract to flow out of the extraction column 80 at the first set flow rate. The inlet end of the extract switching valve 30 is controlled to be connected to the first outlet end, allowing the first extract to flow out of the waste liquid pipeline 170. If the pH value is greater than the preset pH threshold, the second steady flow valve 50 is controlled to open according to the second set valve opening, and the second flowmeter 130 is turned on, allowing the first buffer to flow into the extraction column 80 at the second set flow rate, and causing the extraction column 80 to produce the second extract. At the same time, the inlet end of the extract switching valve 30 is kept connected to the first outlet end, allowing the second extract to flow out of the waste liquid pipeline 170. The purpose of adding the first extract is to remove foreign proteins and other impurities in the initial liquid.

[0056] In step S103 , after the first buffer solution flows into the extraction column 80 for a second preset time, the absorbance detection unit 90 obtains a first sample liquid from the second extraction liquid and obtains a first absorbance of the first sample liquid.

[0057] Here, as an example, the second preset time may be set to three hours, or may be set to other values ​​based on other actual experience or working conditions, which is not limited here.

[0058] In the examples of this application, conventional absorbance detection has the following limitations: First, a narrow measuring range: the effective absorbance detection is low, with an upper limit typically less than 5. Second, readings drift when the absorbance exceeds 1, resulting in reduced measurement accuracy. In actual detection, the absorbance exceeds 10 at the highest concentration. Therefore, for sample liquids with an absorbance greater than 5, the sample liquid is diluted a certain multiple before measurement. To improve absorbance accuracy in high-concentration, complex samples, or for online detection, this application first adjusts the optical path length, employing an adjustable optical pathlength flow cell design. As an example, the flow cell optical pathlength is continuously adjustable from 0.2 to 10 mm. Furthermore, multi-wavelength detection is utilized to achieve an ultra-wide absorbance range of 0-15 nm. As an example, a multi-band ultraviolet to visible light can be utilized, with a detection wavelength range exceeding 200-800 nm. Based on real-time absorbance, adaptive range switching (e.g., switching from high-sensitivity mode to high-range mode) can be performed, effectively distinguishing between absorption signals and scattering interference, significantly reducing signal saturation at high absorbances. Next, a correction term is introduced to account for the scattering effect of highly absorbent samples (e.g., A value > 1.0). For example, polynomial fitting and power law models can be used to introduce correction terms. Through nonlinear correction, the absorbance conversion standard for different ranges is unified, avoiding error jumps caused by segmented detection intervals. Secondly, noise suppression is performed. For example, filtering algorithms (such as Kalman filtering) can be used to reduce interference from noise such as ambient light, bubbles, or suspended particles.

[0059] In the embodiments of the present application, since there is a positive correlation between absorbance and proteolytic enzyme concentration, a nonlinear mapping model between absorbance and proteolytic enzyme can be established using a neural network. The proteolytic enzyme concentration can be accurately predicted by the absorbance data collected in real time. This nonlinear mapping model is applicable to complex biological samples.

[0060] Regarding step S103, in specific implementation, as an example, the following steps may be included: A. After the first buffer solution flows into the extraction column 80 for a second preset time, the input switching end of the inflow switching valve 92 that meets the connection condition is connected to the outlet end of the inflow switching valve 92 to allow the second extraction liquid to flow into the detection pipeline 91.

[0061] In the embodiment of the present application, only a small amount of the second extraction liquid flows into the detection pipeline 91.

[0062] It should be noted that different control modes are adopted according to the configuration of the absorbance detection unit 90: when the detection unit only supports single-channel detection, the connectivity condition is that the first buffer solution continues to flow into the extraction column 80 for a second preset time; when the detection unit has a multi-channel rotation function, if a single extraction column 80 satisfies the first buffer solution inflow time reaching the second preset time, the corresponding input switching end is opened to receive the extract generated by the extraction column 80, that is, the connectivity condition is that the first buffer solution flows into only one extraction column 80 for the second preset time; if multiple extraction columns 80 simultaneously meet the first buffer solution inflow time reaching the second preset time, the input switching end is automatically switched in turn according to the preset priority order and time interval, and the extract generated by each extraction column 80 is received in order, that is, the connectivity condition is that multiple extraction columns 80 simultaneously meet the first buffer solution inflow time reaching the second preset time, reach the set rotation time interval and meet the set priority order.

[0063] B. After the inflow switching valve 92 is connected, the filter 93, the peristaltic pump 94 and the debubbler 95 are controlled to be opened, so that the second extract liquid is free of impurities and bubbles to obtain the first sample liquid, and the first sample liquid is made to flow to the UV detector 96 at a fourth set flow rate.

[0064] C. Continuously obtain the first absorbance of the first sample liquid through the ultraviolet detector 96. As an example, the absorbance of the whole process of enzyme extraction is monitored online in real time. Figure 5 shown.

[0065] Here, while obtaining the first absorbance, the first one-way valve 150 is opened to allow the first sample liquid to flow out of the detection pipeline 91 and out of the waste liquid pipeline 170 .

[0066] In step S104 , if the first absorbance is less than the first absorbance threshold, the third steady flow valve 60 is controlled to open and the second steady flow valve 50 is controlled to close, so that the second buffer solution flows into the extraction column 80 at a third set flow rate, and the extraction column 80 produces a third extract.

[0067] Here, the first absorbance threshold can be set to 6, or it can be set to other values ​​based on other actual experience or working conditions, and there is no limitation here. The first set flow rate and the third set flow rate are consistent, that is, the flow rate of the second buffer solution flowing into the extraction column 80 and the flow rate of the third extract solution flowing out of the extraction column 80 remain basically consistent. As an example, the dynamic trend diagram of the flow rate of the second buffer solution flowing into the extraction column 80 and the flow rate of the third extract solution flowing out of the extraction column 80 is as shown in FIG. Figure 6 When the flow deviation exceeds the allowable range due to real-time fluctuations in pipeline pressure, the opening of the first steady-flow valve 20 or the third steady-flow valve 60 is automatically adjusted to maintain the opening within the set range. The purpose of adding the second buffer is to elute the proteolytic enzyme from the cellulose.

[0068] In this step, specifically, if the first absorbance is less than the first absorbance threshold, the second flow stabilizing valve 50 is controlled to open according to the third set valve opening, and the third flowmeter 140 is opened to allow the third extraction liquid to flow out of the extraction column 80 according to the third set flow rate. At the same time, the inlet end of the extraction liquid switching valve 30 is kept connected to the first outlet end, so that the second extraction liquid flows out from the waste liquid pipeline 170.

[0069] In step S105 , a second sample liquid is obtained from the third extracting liquid by the absorbance detection unit 90 , and a second absorbance of the second sample liquid is obtained.

[0070] Step S106 : If the second absorbance is less than the second absorbance threshold, the extraction liquid switching valve 30 is controlled to switch the channel to obtain the target extraction liquid.

[0071] Here, the second absorbance threshold may be set to 2.3, or may be set to other values ​​based on other practical experience or working conditions, and is not limited here.

[0072] Specifically, if the second absorbance is less than the second absorbance threshold, the inlet end of the extraction liquid switching valve 30 is controlled to communicate with the second outlet end, so that the third extraction liquid and the second sample liquid meeting the collection conditions flow out from the collection pipeline 160 to obtain the target extraction liquid.

[0073] In an embodiment of the present application, if the second absorbance is less than the third absorbance threshold, the extraction liquid switching valve 30 is controlled to switch the channel, and the first cleaning switching valve 180, the second cleaning switching valve 190, the second one-way valve 210 and the first steady flow valve 20 are controlled to open, so that the first cleaning liquid and the second cleaning liquid clean the extraction device, and the liquid after cleaning the extraction device flows out from the waste liquid pipeline 170. Here, the third absorbance threshold can be set to 0.25, or it can be set to other values ​​based on other actual experience or working conditions, and there is no restriction here. The first cleaning liquid can be drinking water or other liquids for cleaning salt crystals, and there is no restriction here. The second cleaning liquid can be purified water or other cleaning liquids, and there is no restriction here.

[0074] Specifically, if the second absorbance of the second sample liquid detected by the absorbance detection unit 90 is less than the third absorbance threshold, the supply of the second buffer solution is closed, the extraction is terminated, and the inlet end of the extraction liquid switching valve 30 is controlled to be connected to the first outlet end; the first cleaning switching valve 180 is controlled to open, mainly to clean the first steady flow valve 20, the first flow meter 120, the second steady flow valve 50, the second flow meter 130, the third steady flow valve 60 and the third flow meter 140, to prevent the salt components remaining on the steady flow valve and the flow meter from crystallizing and getting stuck in the flow meter during the extraction process, causing the flow meter to malfunction; the second cleaning switching valve 190 is controlled to open, to clean the extraction column 80 and the extraction liquid outflow pipeline; the second one-way valve 210 is controlled to open, to clean the absorbance detection unit 90.

[0075] See also Figure 7 and Figure 8 , Figure 7 This is a schematic diagram comparing the control time between online detection and manual offline detection. Figure 8 Schematic diagram of the comparison of lumbrokinase quality between online detection and manual detection.

[0076] like Figure 7 As shown, the control time of online detection and manual offline detection in the lumbrokinase extraction process was shortened by at least 1230 minutes, reducing detection costs and labor costs.

[0077] like Figure 8 As shown in the figure, the mass fluctuation of lumbrokinase ranged from 1.5% to 0.92%, indicating a more stable mass. The mass yield of lumbrokinase decreased from 12.2% to 4.01%, indicating a reduced volatility. Lumbrokinase mass was measured and calculated using the relative standard deviation (RSD) of the unit potency, while the mass yield of lumbrokinase was measured and calculated using the relative standard deviation.

[0078] An embodiment of the present application provides an extraction device method. Through the extraction method, the present application can monitor the pH value, flow rate and absorbance of the extract of each extraction column 80 in real time online, and automatically complete buffer switching, start and stop control of target extraction liquid collection, and balance adjustment of inlet and outlet liquid flow based on the detection data, thereby realizing continuous automated extraction of protein hydrolase and autonomous steady-state regulation of the extraction process, ensuring the acquisition of protein hydrolase with stable quality, improving the purification efficiency of protein hydrolase, shortening detection time, reducing manual intervention, and lowering manual operation costs.

[0079] See also Figure 9 , Figure 9 A controller 110 is provided in an embodiment of the present application.

[0080] like Figure 9 As shown in FIG, the controller 110 includes: The pH acquisition module 1101 acquires the pH value of the first extract produced by the extraction column 80 through the pH detector 10; wherein the first extract is produced by adsorbing the initial liquid in the extraction column 80; The first buffer inflow module 1102 controls the first steady flow valve 20 to open and the extraction liquid switching valve 30 to connect after the initial liquid in the extraction column 80 is adsorbed for a first preset time, so that the liquid that does not meet the collection conditions flows out of the extraction column 80 at a first set flow rate. If the pH value is greater than a preset pH threshold, the first steady flow valve 20 is controlled to open, so that the first buffer flows into the extraction column 80 at a second set flow rate, and the extraction column 80 produces a second extract. The first absorbance acquisition module 1103 is configured to acquire a first sample liquid from the second extraction liquid through the absorbance detection unit 90 after the first buffer solution flows into the extraction column 80 for a second preset time, and acquire a first absorbance of the first sample liquid; The second buffer solution flows into the module 1104. If the second absorbance is less than the first absorbance threshold, the third steady flow valve 60 is controlled to open and the first steady flow valve 20 is controlled to close, so that the second buffer solution flows into the extraction column 80 at a third set flow rate, and the extraction column 80 produces a third extract. The second absorbance acquisition module 1105 acquires a second sample liquid from the third extract through the absorbance detection unit 90 and acquires a second absorbance of the second sample liquid; The collecting module 1106 controls the extraction liquid switching valve 30 to switch the channel to obtain the target extraction liquid if the second absorbance is less than the second absorbance threshold.

[0081] Furthermore, the first absorbance acquisition module 1103 is specifically used to: After the first buffer solution flows into the extraction column 80 for a second preset time, the input switching end of the inflow switching valve 92 that meets the connection condition is connected to the outlet end of the inflow switching valve 92, so that the second extraction liquid flows into the detection pipeline 91; After the inflow switching valve 92 is connected, the filter 93, the peristaltic pump 94 and the debubbler 95 are controlled to be opened, so that the second extract liquid is free of impurities and bubbles to obtain the first sample liquid, and the first sample liquid is made to flow to the UV detector 96 at a fourth set flow rate; The first absorbance of the first sample liquid is obtained by the ultraviolet detector 96 .

[0082] Furthermore, the controller 110 further includes: Cleaning module 1107, if the second absorbance is less than the third absorbance threshold, controls the extraction liquid switching valve 30 to switch the channel, and controls the first cleaning switching valve 180, the second cleaning switching valve 190, the second one-way valve 210 and the first steady flow valve 20 to open, so that the first cleaning liquid and the second cleaning liquid clean the extraction device, and the liquid after cleaning the extraction device flows out from the waste liquid pipeline 170.

[0083] Those skilled in the art can clearly understand that, for the convenience and simplicity of description, the specific working process of the system and device described above can refer to the corresponding process in the aforementioned method embodiment, and will not be repeated here. In the several embodiments provided in this application, it should be understood that the disclosed system, device and method can be implemented in other ways. The device embodiments described above are merely schematic. For example, the division of the units is only a logical function division. There may be other division methods in actual implementation. For example, multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. Another point is that the mutual coupling or direct coupling or communication connection shown or discussed can be through some communication interfaces, indirect coupling or communication connection of devices or units, which can be electrical, mechanical or other forms.

[0084] The units described as separate components may or may not be physically separate, and the components shown as units may or may not be physical units, that is, they may be located in one place or distributed across multiple network units. Some or all of these units may be selected to achieve the purpose of this embodiment according to actual needs.

[0085] In addition, each functional unit in each embodiment of the present application may be integrated into one processing unit, or each unit may exist physically separately, or two or more units may be integrated into one unit.

[0086] If the functions are implemented in the form of software functional units and sold or used as independent products, they can be stored in a non-volatile computer-readable storage medium that is executable by a processor. Based on this understanding, the technical solution of the present application, or the part that contributes to the prior art, or the part of the technical solution, can be embodied in the form of a software product. The computer software product is stored in a storage medium and includes several instructions for enabling a computer device (which can be a personal computer, server, or network device, etc.) to execute all or part of the steps of the method described in each embodiment of the present application. The aforementioned storage medium includes various media that can store program codes, such as a USB flash drive, a mobile hard disk, a read-only memory (ROM), a random access memory (RAM), a magnetic disk, or an optical disk.

[0087] The above are only specific embodiments of the present application, but the scope of protection of this application is not limited thereto. Any changes or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in this application should be included in the scope of protection of this application. Therefore, the scope of protection of this application should be based on the scope of protection of the claims.

Claims

1. A liquid extraction device, characterized in that: The extraction device comprises: a pH detector, a first steady flow valve, an extraction liquid switching valve, an extraction liquid outflow pipeline, a second steady flow valve, a third steady flow valve, an eluent inflow pipeline, an extraction column, an absorbance detection unit and a controller; The pH detector, the first steady flow valve and the extraction liquid switching valve are all arranged on the extraction liquid outflow pipeline, the second steady flow valve and the third steady flow valve are both arranged on the eluent inflow pipeline, the input switching end of the extraction column is connected to the outlet end of the eluent inflow pipeline, the outlet end of the extraction column is connected to the inlet end of the extraction liquid outflow pipeline, the extraction liquid outflow pipeline is connected to the absorbance detection unit, and the extraction liquid switching valve, the pH detector, the first steady flow valve, the second steady flow valve, the third steady flow valve and the absorbance detection unit are all connected to the controller.

2. The liquid extraction device according to claim 1, characterized in that The eluent inflow pipeline includes: a first buffer solution pipeline and a second buffer solution pipeline; the extraction liquid outflow pipeline includes: a first outflow pipeline; the extraction device also includes: a first flow meter, a second flow meter and a third flow meter; the input switching end of the extraction column includes: a first inlet end and a second inlet end; The first steady flow valve, the first flow meter and the extraction liquid switching valve are arranged on the first outflow pipeline, the second steady flow valve and the second flow meter are both arranged on the first buffer solution pipeline, the third steady flow valve and the third flow meter are arranged on the second buffer solution pipeline, the outlet end of the first buffer solution pipeline is connected to the first inlet end of the extraction column, the outlet end of the second buffer solution pipeline is connected to the second inlet end of the extraction column, the outlet end of the extraction column is connected to the inlet end of the first outflow pipeline, and the first flow meter, the second flow meter and the third flow meter are all connected to the controller.

3. The liquid extraction device according to claim 2, characterized in that The extraction device further includes: a first one-way valve; the extraction liquid outflow pipeline includes: a second outflow pipeline and a third outflow pipeline; the absorbance detection unit includes: a detection pipeline, an inflow switching valve, a filter, a peristaltic pump, a debubbler, a UV detector and an outflow switching valve; A first one-way valve is arranged on the third outflow pipeline, and the inflow switching valve, the filter, the peristaltic pump, the debubbler, the ultraviolet detector and the outflow switching valve are all arranged on the detection pipeline. The inlet end of the second outflow pipeline is connected to the outlet end of the first flowmeter, the outlet end of the second outflow pipeline is connected to the input switching end of the inflow switching valve, the output switching end of the outflow switching valve is connected to the inlet end of the third outflow pipeline, and the outlet end of the third outflow pipeline is connected to the inlet end of the extraction liquid switching valve. The first one-way valve, the inflow switching valve, the filter, the peristaltic pump, the debubbler, the ultraviolet detector and the outflow switching valve are all connected to the controller.

4. The liquid extraction device according to claim 1, characterized in that The liquid extraction device further comprises: a collection pipeline and a waste liquid pipeline; The first outlet end of the extraction liquid switching valve is communicated with the inlet end of the waste liquid pipeline, and the second outlet end of the extraction liquid switching valve is communicated with the inlet end of the collection pipeline.

5. The liquid extraction device according to claim 1, characterized in that The eluent inflow pipeline further includes: a first cleaning liquid inflow pipeline; the liquid extraction device further includes: a first cleaning switching valve; The first cleaning switching valve is arranged on the first cleaning liquid inflow pipeline, the inlet end of the first cleaning switching valve is connected to the inlet end of the first cleaning liquid inflow pipeline, the first outlet end of the first cleaning switching valve is connected to the inlet end of the second flow stabilizing valve, the second outlet end of the first cleaning switching valve is connected to the inlet end of the third flow stabilizing valve, and the first cleaning switching valve is connected to the controller.

6. The liquid extraction device according to claim 1, characterized in that The eluent inflow pipeline further includes: a second cleaning liquid inflow pipeline; the liquid extraction device further includes: a second cleaning switching valve, a third cleaning liquid inflow pipeline, and a second one-way valve; the input switching end of the extraction column includes: a third inlet end; The second cleaning switching valve is arranged on the second cleaning liquid inflow pipeline, and the second one-way valve is arranged on the third cleaning liquid inflow pipeline. The inlet end of the second cleaning switching valve is connected with the inlet end of the second cleaning liquid inflow pipeline, the first outlet end of the second cleaning liquid inflow pipeline is connected with the third inlet end of the extraction column, the second outlet end of the second cleaning liquid inflow pipeline is connected with the inlet end of the third cleaning liquid inflow pipeline, and the outlet end of the third cleaning liquid inflow pipeline is connected with the outlet end of the inflow switching valve; the second cleaning switching valve and the second one-way valve are both connected to the controller.

7. A liquid extraction method, characterized in that: The extraction method is applied to the extraction device according to any one of claims 1 to 6, and the extraction method comprises: Obtaining the pH value of a first extract produced by the extraction column through a pH detector; wherein the first extract is produced by adsorbing the initial liquid in the extraction column; After the initial liquid in the extraction column is adsorbed for a first preset time, the first steady flow valve is controlled to open and the extraction liquid switching valve is controlled to be connected, so that the liquid that does not meet the collection condition flows out of the extraction column at a first set flow rate, and if the pH value is greater than a preset pH threshold, the second steady flow valve is controlled to open, so that the first buffer solution flows into the extraction column at a second set flow rate, and the extraction column produces a second extract; After the first buffer solution flows into the extraction column for a second preset time, obtaining a first sample liquid from the second extraction liquid by the absorbance detection unit, and obtaining a first absorbance of the first sample liquid; If the first absorbance is less than the first absorbance threshold, the third steady flow valve is controlled to open and the second steady flow valve is controlled to close, so that the second buffer solution flows into the extraction column at a third set flow rate, and the extraction column produces a third extract; obtaining a second sample liquid from the third extract liquid through an absorbance detection unit, and obtaining a second absorbance of the second sample liquid; If the second absorbance is less than the second absorbance threshold, the extraction liquid switching valve is controlled to switch the channel to obtain the target extraction liquid.

8. The liquid extraction method according to claim 7, characterized in that After the first buffer solution flows into the extraction column for a second preset time, the first sample liquid is obtained from the second extraction liquid by the absorbance detection unit, and the first absorbance of the first sample liquid is obtained, including: After the first buffer solution flows into the extraction column for a second preset time, connecting the input switching end of the inflow switching valve that meets the connection condition with the outlet end of the inflow switching valve to allow the second extraction solution to flow into the detection pipeline; After the inflow switching valve is connected, the filter, the peristaltic pump and the debubbler are controlled to be opened, so that the second extract liquid is free of impurities and bubbles to obtain the first sample liquid, and the first sample liquid is made to flow to the ultraviolet detector at a fourth set flow rate; A first absorbance of the first sample liquid is obtained by an ultraviolet detector.

9. The liquid extraction method according to claim 7, characterized in that: The extraction method further comprises: If the second absorbance is less than the third absorbance threshold, the extraction liquid switching valve is controlled to switch the channel, and the first cleaning switching valve, the second cleaning switching valve, the second one-way valve and the first steady flow valve are controlled to open, so that the first cleaning liquid and the second cleaning liquid clean the extraction device, and the liquid after cleaning the extraction device flows out from the waste liquid pipeline.

10. A controller, characterized in that: The controller includes: A pH acquisition module, which acquires the pH value of a first extract produced by the extraction column through a pH detector; wherein the first extract is produced by adsorbing an initial liquid in the extraction column; The first buffer solution flows into the module. After the initial liquid in the extraction column is adsorbed for a first preset time, the first steady flow valve is controlled to open and the extraction liquid switching valve is controlled to be connected, so that the liquid that does not meet the collection conditions flows out of the extraction column at a first set flow rate. If the pH value is greater than a preset pH threshold, the first steady flow valve is controlled to open, so that the first buffer solution flows into the extraction column at a second set flow rate, and the extraction column produces a second extract; a first absorbance acquisition module, which acquires a first sample liquid from the second extracting liquid through an absorbance detection unit after the first buffer solution flows into the extraction column for a second preset time, and acquires a first absorbance of the first sample liquid; The second buffer solution flows into the module. If the second absorbance is less than the first absorbance threshold, the third steady flow valve is controlled to open and the first steady flow valve is controlled to close, so that the second buffer solution flows into the extraction column at a third set flow rate, and the extraction column produces a third extract; a second absorbance acquisition module, which acquires a second sample liquid from the third extract through an absorbance detection unit and acquires a second absorbance of the second sample liquid; The collection module controls the extraction liquid switching valve to switch the channel to obtain the target extraction liquid if the second absorbance is less than the second absorbance threshold.

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