Blood cell analyzer and blood cell analysis method

Abstract

The invention discloses a blood cell analyzer and a blood cell analysis method. A liquid storage assembly of the blood cell analyzer comprises a liquid storage cavity, and the height of an inlet of the liquid storage cavity is smaller than that of an outlet of the liquid storage cavity; the first end of a temporary storage mechanism of the liquid path emptying assembly is connected with a pressure supply mechanism, and the second end of the temporary storage mechanism is connected with an inlet of the liquid storage assembly through a first control valve. The processor is used for controlling the pressure supply mechanism to switch the temporary storage mechanism to a negative pressure state in response to a received liquid discharge instruction, and the processor is further used for controlling the first control valve to conduct the liquid storage assembly and the temporary storage mechanism, so that liquid in the liquid storage cavity is discharged through a pipeline between the first control valve and the liquid storage assembly. According to the application, the liquid in the liquid storage cavity can be emptied through the pipeline between the first control valve and the liquid storage assembly by virtue of the temporary storage mechanism in the negative pressure state, so that a residual reagent in the liquid storage cavity is removed, the blockage fault caused by evaporation and crystallization of the reagent is reduced or avoided, the service life of the hematology analyzer is prolonged, and the reliability of the hematology analyzer is improved.

Description

Technical Field

[0001] The present application relates to the field of in vitro diagnostic technology, and in particular to a blood cell analyzer and a blood cell analysis method. Background Art

[0002] In hematology analyzers, in order to better expel bubbles from the reagents, the liquid path structure of some liquid storage components adopts a bottom-in, top-out structure for liquid to pass through, so that the liquid flows from bottom to top to another component, thereby reducing the impact of bubbles on analyzer detection.

[0003] However, when the liquid storage component adopts a bottom-in and top-out structure, since the outlet level of the liquid in the liquid storage cavity is higher than the inlet level, when the liquid in the liquid storage cavity is discharged from its outlet, some reagent will always remain at the bottom of the liquid storage cavity. When the reagent remains in the cavity for too long, the moisture in the reagent will gradually evaporate and crystallize. The crystallization can easily lead to blockage of the cavity, flow channel and liquid valve components, resulting in reduced reliability of the blood cell analyzer. Summary of the Invention

[0004] To solve the above technical problems, the present application provides a blood cell analyzer and a blood cell analysis method.

[0005] To solve the above problems, the present application provides a blood cell analyzer, including a liquid storage component, a liquid circuit emptying component and a processor; the liquid storage component includes a liquid storage cavity, the liquid storage cavity has an inlet and an outlet, and the height of the inlet is smaller than the height of the outlet; the liquid circuit emptying component includes a temporary storage mechanism, a pressure supply mechanism and a first control valve, the first end of the temporary storage mechanism is connected to the pressure supply mechanism through a pipeline, and the second end of the temporary storage mechanism is connected to the inlet of the liquid storage component through the first control valve; the processor is connected to the liquid circuit emptying component; wherein, the processor is used to control the pressure supply mechanism to switch the temporary storage mechanism to a negative pressure state in response to receiving a liquid discharge instruction, and the processor is also used to control the first control valve to connect the liquid storage component and the temporary storage mechanism, so that the liquid in the liquid storage cavity is discharged through the pipeline between the first control valve and the liquid storage component.

[0006] Optionally, the above-mentioned blood cell analyzer also includes a liquid collection component, which is connected to the outlet of the above-mentioned liquid storage component; the above-mentioned processor is used to: in response to receiving the above-mentioned drainage instruction, control the above-mentioned first control valve to connect the above-mentioned liquid storage component and the above-mentioned temporary storage mechanism, and first control the above-mentioned pressure supply mechanism to switch the above-mentioned temporary storage mechanism to a positive pressure state, so that the liquid in the above-mentioned liquid storage cavity is discharged to the above-mentioned liquid collection component through the above-mentioned outlet; then control the above-mentioned first control valve to connect the above-mentioned liquid storage component and the above-mentioned temporary storage mechanism, and control the above-mentioned pressure supply mechanism to switch the above-mentioned temporary storage mechanism to a negative pressure state, so that the liquid in the above-mentioned liquid storage cavity is discharged through the above-mentioned inlet, the pipeline between the above-mentioned first control valve and the above-mentioned liquid storage component.

[0007] Optionally, the above-mentioned blood cell analyzer includes a reagent supply assembly, the above-mentioned temporary storage mechanism includes a power pump, the first end of the above-mentioned first control valve is connected to the above-mentioned power pump through the above-mentioned pipeline, the second end of the above-mentioned first control valve is connected to the above-mentioned reagent supply assembly through the above-mentioned pipeline, and the third end of the above-mentioned first control valve is connected to the above-mentioned liquid storage assembly through the above-mentioned pipeline; wherein the above-mentioned processor is used to: in response to receiving the above-mentioned discharge instruction, control the above-mentioned pressure supply mechanism to switch the above-mentioned power pump to the above-mentioned negative pressure state; control the above-mentioned first control valve to connect the above-mentioned power pump and the above-mentioned liquid storage assembly, so that the above-mentioned power pump absorbs the liquid in the above-mentioned pipeline between the above-mentioned liquid storage cavity and the above-mentioned first control valve; control the above-mentioned pressure supply mechanism to switch the above-mentioned power pump to the positive pressure state, and control the above-mentioned first control valve to connect the above-mentioned power pump and the above-mentioned reagent supply assembly, so that the above-mentioned power pump discharges the above-mentioned absorbed liquid to the above-mentioned reagent supply assembly.

[0008] Optionally, the reagent supply assembly is used to provide a first reagent; before the power pump absorbs the liquid in the pipeline between the liquid storage chamber and the first control valve, the processor is also used to: control the first control valve to connect the power pump and the reagent supply assembly, and control the pressure supply mechanism to switch the temporary storage mechanism to a negative pressure state, so that the power pump absorbs the first reagent of the reagent supply assembly; control the first control valve to connect the power pump and the liquid storage assembly, and control the pressure supply mechanism to switch the temporary storage mechanism to the positive pressure state, so that the power pump discharges the absorbed first reagent into the liquid storage assembly to clean the liquid storage assembly.

[0009] Optionally, the above-mentioned blood cell analyzer also includes an incubation component, the above-mentioned reagent supply component is used to provide a second reagent, and the above-mentioned incubation component is connected to the outlet of the above-mentioned liquid storage component; before the above-mentioned power pump absorbs the liquid in the above-mentioned pipeline between the above-mentioned liquid storage cavity and the above-mentioned first control valve, the above-mentioned processor is also used to: when the above-mentioned first control valve connects the above-mentioned power pump and the above-mentioned reagent supply component and the above-mentioned temporary storage mechanism is in the above-mentioned negative pressure state, control the above-mentioned power pump to absorb the second reagent of the above-mentioned reagent supply component; when the above-mentioned first control valve connects the above-mentioned power pump and the above-mentioned liquid storage component and the above-mentioned temporary storage mechanism is in the above-mentioned positive pressure state, control the above-mentioned power pump to discharge the absorbed above-mentioned second reagent into the above-mentioned liquid storage component, so as to realize the replenishment of the above-mentioned second reagent into the liquid storage component; and / or, control the above-mentioned first control valve to connect the above-mentioned liquid storage component and the above-mentioned power pump, and control the above-mentioned pressure supply mechanism to switch the above-mentioned power pump to the above-mentioned positive pressure state, so that the liquid in the above-mentioned liquid storage cavity is discharged to the above-mentioned incubation component through the above-mentioned outlet.

[0010] Optionally, the above-mentioned blood cell analyzer includes a reagent supply assembly, the above-mentioned temporary storage mechanism includes a liquid storage tank, a power pump and a second control valve, the above-mentioned power pump is connected to the above-mentioned liquid storage tank through the above-mentioned second control valve, the above-mentioned liquid storage tank is connected to the above-mentioned liquid storage assembly through the above-mentioned first control valve, and the above-mentioned pressure supply mechanism is respectively connected to the above-mentioned liquid storage tank and the above-mentioned power pump; wherein, the above-mentioned processor is used to: in response to receiving the above-mentioned drainage instruction, control the above-mentioned pressure supply mechanism to switch the above-mentioned power pump to the above-mentioned negative pressure state, and control the above-mentioned second control valve to connect the above-mentioned liquid storage tank and the above-mentioned power pump, so that the above-mentioned power pump absorbs the air in the above-mentioned liquid storage tank; when the air pressure in the above-mentioned liquid storage tank reaches a preset condition, control the above-mentioned first control valve to connect the above-mentioned liquid storage tank and the above-mentioned liquid storage assembly, so that the liquid in the above-mentioned liquid storage cavity is discharged to the above-mentioned liquid storage tank through the pipeline between the above-mentioned inlet, the above-mentioned first control valve and the above-mentioned liquid storage assembly.

[0011] Optionally, the liquid circuit emptying component further includes a third control valve and a drainage branch, wherein the first end of the third control valve is connected to the pipeline between the liquid storage tank and the first control valve, and the second end of the third control valve is connected to the drainage branch; the processor is also used to control the first control valve to cut off and control the third control valve to be connected, so that the liquid in the liquid storage tank is discharged to the drainage branch.

[0012] Optionally, the pressure supply mechanism includes a first pressure source, a second pressure source and a fourth control valve, the first pressure source is connected to the power pump, the second pressure source is connected to the liquid storage tank through the fourth control valve, the second pressure source is used to provide positive pressure for the liquid storage tank, the blood cell analyzer further includes a liquid collection component, the liquid collection component is connected to the outlet of the liquid storage component; wherein, before the liquid in the liquid storage cavity is discharged to the liquid storage tank through the pipeline between the inlet, the first control valve and the liquid storage component, the processor is used to control the first control valve to conduct the liquid storage in response to receiving the discharge instruction component and the above-mentioned liquid storage tank, and controls the above-mentioned fourth control valve to be connected, so that the above-mentioned liquid storage tank discharges the liquid in the above-mentioned liquid storage cavity through the above-mentioned outlet to the above-mentioned liquid collecting component under the positive pressure of the second pressure source; before the liquid in the above-mentioned liquid storage cavity is discharged to the above-mentioned liquid storage tank through the above-mentioned inlet, the pipeline between the above-mentioned first control valve and the above-mentioned liquid storage component, the above-mentioned processor is also used to respond to receiving the above-mentioned discharge instruction, control the above-mentioned second control valve to connect the above-mentioned power pump and the above-mentioned liquid storage tank, and control the above-mentioned first pressure source to switch the above-mentioned power pump to a positive pressure state, so that the above-mentioned power pump discharges the absorbed reagent to the above-mentioned liquid storage component to clean the above-mentioned liquid storage component.

[0013] Optionally, the liquid circuit emptying assembly further includes a waste liquid storage mechanism, wherein the first end of the second control valve is connected to the power pump via the pipeline, the second end of the second control valve is connected to the liquid storage tank via the pipeline, and the third end of the second control valve is connected to the waste liquid storage mechanism via the pipeline; wherein the processor is configured to: in response to receiving the liquid discharge instruction, first control the second pressure source to switch the power pump to a positive pressure state, and then control the second control valve to conduct electricity between the power pump and the waste liquid storage mechanism, so as to discharge the liquid in the power pump into the waste liquid storage mechanism; Then, the second pressure source is controlled to switch the power pump to the negative pressure state, and the second control valve is controlled to connect the liquid storage tank and the power pump, so that the power pump absorbs the air in the liquid storage tank; the steps of controlling the second pressure source to switch the power pump to the positive pressure state, controlling the second control valve to connect the power pump and the waste liquid storage mechanism, and controlling the second pressure source to switch the power pump to the negative pressure state, and controlling the second control valve to connect the liquid storage tank and the power pump are repeated until the air pressure in the liquid storage tank reaches the preset condition.

[0014] To solve the above problems, the present application provides a blood cell analysis method, which is applied to the above-mentioned blood cell analyzer. The above-mentioned blood cell analysis method includes: in response to receiving a discharge instruction, switching the temporary storage mechanism to a positive pressure state, controlling the first control valve to connect the liquid storage component and the above-mentioned temporary storage mechanism, driving the reagent into the above-mentioned liquid storage component to clean the above-mentioned liquid storage component; controlling the liquid in the above-mentioned liquid storage component to be discharged from the outlet of the above-mentioned liquid storage component; switching the temporary storage mechanism to a negative pressure state, controlling the first control valve to connect the liquid storage component and the temporary storage mechanism, so that the liquid in the above-mentioned liquid storage component is discharged through the inlet of the above-mentioned liquid storage component and the pipeline between the above-mentioned first control valve and the above-mentioned liquid storage component.

[0015] The present application provides a blood cell analyzer and a blood cell analysis method, wherein the liquid storage component of the blood cell analyzer includes a liquid storage cavity, the liquid storage cavity having an inlet and an outlet, the height of the inlet being less than the height of the outlet; the first end of the temporary storage mechanism of the liquid path emptying component is connected to the pressure supply mechanism through a pipeline, and the second end of the temporary storage mechanism is connected to the inlet of the liquid storage component through a first control valve; the processor is used to control the pressure supply mechanism to switch the temporary storage mechanism to a negative pressure state in response to receiving a liquid discharge instruction, and the processor is also used to control the first control valve to connect the liquid storage component and the temporary storage mechanism so that the liquid in the liquid storage cavity is discharged through the pipeline between the first control valve and the liquid storage component. Therefore, the present application can empty the liquid in the liquid storage cavity through the pipeline between the first control valve and the liquid storage component through the temporary storage mechanism in a negative pressure state to clear the residual reagent in the liquid storage cavity, reduce or avoid blockage failures caused by reagent evaporation and crystallization, and improve the service life and reliability of the blood cell analyzer. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present application. For those skilled in the art, other drawings can be obtained based on these drawings without inventive efforts. Among them: Figure 1 This is a schematic structural diagram of the first embodiment of the blood cell analyzer provided by the present application; Figure 2 is a structural schematic diagram of a second embodiment of the blood cell analyzer provided by the present application; Figure 3 1 is a schematic structural diagram of a third embodiment of a blood cell analyzer provided by the present application; Figure 4 1 is a schematic structural diagram of a fourth embodiment of a blood cell analyzer provided by the present application; Figure 5 1 is a flow chart of an embodiment of the blood cell analysis method provided in the present application.

[0017] Among them, 10, liquid storage component; 11, liquid storage cavity; 20, liquid circuit emptying component; 21, temporary storage mechanism; 211, power pump; 212, liquid storage tank; 213, second control valve; 22, pressure supply mechanism; 221, fifth control valve; 222, first pressure source; 2221, positive pressure source; 2222, negative pressure source; 223, second pressure source; 224, fourth control valve; 23, first control valve; 24, third control valve; 25, drainage branch; 26, waste liquid storage mechanism; 27, sixth control valve; 30, processor; 40, liquid collection component; 50, reagent supply component; 51, first storage bottle; 52, second storage bottle; 53, third storage bottle; 60, incubation component. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments of this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0019] It should be noted that if the embodiments of the present application involve directional indications (such as up, down, left, right, front, back, etc.), such directional indications are only used to explain the relative position relationship, movement status, etc. between the various components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0020] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present application, the descriptions of "first", "second", etc. are only for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of such features. In addition, the technical solutions between the various embodiments can be combined with each other, but they must be based on the fact that they can be implemented by ordinary technicians in this field. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by this application.

[0021] Specifically, in one embodiment of the related technology, in order to ensure the accuracy and reliability of the detection, the blood cell analyzer may include a reagent preheating component, which is used to preheat the reagent to a certain temperature before use, and to heat it in order to ensure the volume of the reagent after heating.

[0022] In another embodiment of the related art, the detection assembly of a hematology analyzer may include a sheath flow cell. This cell is used to envelop a sample solution with sheath fluid, ensuring that the sample solution passes through the detection site at a stable speed and width for detection and counting. To ensure pressure and flow rate at the detection site, a liquid reservoir is designed within the sheath fluid flow channel of the sheath flow cell to stabilize the sheath fluid pressure and flow rate in advance.

[0023] Therefore, the reagent preheating assembly and / or sheath flow cell described above in the related art can be referred to as a liquid reservoir assembly. To effectively expel bubbles from the reagent, the liquid reservoir assembly is typically designed with a bottom-in, top-out structure, so that the fluid moves from bottom to top, causing bubbles in the fluid to float upward and be expelled, thereby reducing the impact of bubbles on the analyzer's detection. However, with the above-mentioned structure and the bottom-up movement of the fluid, some reagent will always remain in the cavity of the liquid reservoir assembly and cannot be expelled. If the reagent remains in the cavity for too long, it will cause crystallization.

[0024] In order to prevent the evaporation and crystallization of reagents inside the hematology analyzer, the related art usually requires that the reagents inside the instrument be emptied before using pure water or distilled water to clean the reagents in the pipes and cavities. However, when using water to clean the liquid storage component, the problem of water not being able to be discharged still exists due to the bottom-in and top-out structure. The related art also adopts the method of continuously cleaning the liquid storage component with water to dilute the reagents to reduce the probability of crystallization blockage, but this solution still cannot completely empty the reagents in the liquid storage component, the operation is complicated and the reliability is low.

[0025] In view of this, the present application embodiment first proposes a blood cell analyzer. Figure 1 , Figure 1 Schematic diagram of the structure of the first embodiment of the blood cell analyzer provided by this application. Figure 1 As shown, in this embodiment, the blood cell analyzer includes a liquid storage component 10, a liquid path emptying component 20 and a processor 30.

[0026] The liquid storage component 10 includes a liquid storage cavity 11, which has an inlet and an outlet, and the height of the inlet is smaller than the height of the outlet; the liquid circuit emptying component 20 includes a temporary storage mechanism 21, a pressure supply mechanism 22 and a first control valve 23, the first end of the temporary storage mechanism 21 is connected to the pressure supply mechanism 22 through a pipeline, and the second end of the temporary storage mechanism 21 is connected to the inlet of the liquid storage component 10 through the first control valve 23; the processor 30 is connected to the liquid circuit emptying component 20; wherein, the processor 30 is used to control the pressure supply mechanism 22 to switch the temporary storage mechanism 21 to a negative pressure state in response to receiving a discharge instruction, and the processor 30 is also used to control the first control valve 23 to connect the liquid storage component 10 and the temporary storage mechanism 21, so that the liquid in the liquid storage cavity 11 is discharged through the pipeline between the first control valve 23 and the liquid storage component 10.

[0027] Specifically, the above-mentioned liquid circuit emptying assembly 20 includes pipes, valves, pumps and other control components connected to the inlet of the liquid storage assembly 10. The pressure supply mechanism 22 is used to provide a preset air pressure environment for the temporary storage mechanism 21, so that the temporary storage mechanism 21 is in a positive pressure state or a negative pressure state. When the pressure supply mechanism 22 switches the temporary storage mechanism 21 to a negative pressure state, the first control valve 23 conducts electricity between the liquid storage assembly 10 and the temporary storage mechanism 21, that is, the pipe between the temporary storage mechanism 21 and the inlet of the liquid storage assembly 10 can be placed in a negative pressure environment. The liquid in the liquid storage cavity 11 can be sucked out by the negative pressure through the inlet and discharged through the pipe between the first control valve 23 and the liquid storage assembly 10.

[0028] At this time, the processor 30 is used to change the pressure state of the pipeline by controlling at least one of the temporary storage mechanism 21, the pressure supply mechanism 22, and the first control valve 23, so as to control the liquid flow of the liquid storage assembly 10. The processor 30 can be, but is not limited to, a CPU (Central Processing Unit), an integrated circuit chip, a general-purpose processor, a digital signaling processor (DSP), an application-specific integrated circuit (ASIC), a field-programmable gate array (FPGA), or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, or other devices with signal processing capabilities.

[0029] The liquid storage chamber 11 of the liquid storage assembly 10 is provided with an inlet and an outlet. When the hematology analyzer is placed on a table or on the floor, the height between the inlet and the floor is less than the height between the outlet and the floor; alternatively, if the hematology analyzer includes a frame, the height between the inlet and the base plane of the frame is less than the height between the outlet and the base plane. When the liquid storage chamber 11 is used to store reagents, the reagents enter the liquid storage chamber 11 through the inlet. When the hematology analyzer needs to use the reagents stored in the liquid storage chamber 11, the reagents flow out of the liquid storage chamber 11 through the outlet to a designated location.

[0030] In an embodiment of the present application, the liquid storage assembly 10 of the hematology analyzer includes a liquid storage chamber 11 having an inlet and an outlet, the inlet being lower than the outlet. A first end of a temporary storage mechanism 21 of a liquid drain assembly 20 is connected to a pressure supply mechanism 22 via a pipeline, and a second end of the temporary storage mechanism 21 is connected to the inlet of the liquid storage assembly 10 via a first control valve 23. A processor 30 is configured to, in response to receiving a drain instruction, control the pressure supply mechanism 22 to switch the temporary storage mechanism 21 to a negative pressure state. The processor 30 is also configured to control the first control valve 23 to connect the liquid storage assembly 10 and the temporary storage mechanism 21, so that the liquid in the liquid storage chamber 11 is drained through the pipeline between the first control valve 23 and the liquid storage assembly 10. Therefore, in this embodiment, the liquid in the liquid storage chamber 11 can be drained through the pipeline between the first control valve 23 and the liquid storage assembly 10 via the temporary storage mechanism 21 in a negative pressure state, thereby clearing residual reagent in the liquid storage chamber 11, reducing or avoiding blockage caused by reagent evaporation and crystallization, and improving the service life and reliability of the hematology analyzer.

[0031] In some embodiments, see Figure 2 and Figure 3 , Figure 2 is a structural diagram of the second embodiment of the blood cell analyzer provided by this application, Figure 3 FIG. 1 is a schematic diagram of the structure of the third embodiment of the blood cell analyzer provided by this application. Figure 2-Figure 3 As shown, the hematology analyzer further includes a liquid collection assembly 40, which is connected to the outlet of the liquid storage assembly 10. In response to receiving a liquid discharge instruction, the processor 30 is configured to control the first control valve 23 to connect the liquid storage assembly 10 and the temporary storage mechanism 21, and first control the pressure supply mechanism 22 to switch the temporary storage mechanism 21 to a positive pressure state, so that the liquid is discharged through the outlet to the liquid collection assembly 40; then control the first control valve 23 to connect the liquid storage assembly 10 and the temporary storage mechanism 21, and control the pressure supply mechanism 22 to switch the temporary storage mechanism 21 to a negative pressure state, so that the liquid is discharged through the pipeline between the inlet, the first control valve 23, and the liquid storage assembly 10.

[0032] Specifically, the liquid collection component 40 is used to collect the reagents of the liquid storage component 10. In one embodiment, Figure 2 As shown, when the liquid storage component 10 is used as a reagent preheating device to preheat the reagent, the liquid collection component 40 can be some components that use the heated reagent. For example, the liquid collection component 40 is an incubation component 60, and the liquid collection component 40 is used to incubate the sample with the reagent of the liquid storage component 10. In another embodiment, as Figure 3 As shown, the liquid storage component 10 is used as a sheath flow pool to store part of the sheath liquid to ensure the stability of the sheath liquid during the sheath flow process. At this time, the liquid collection component 40 can be a waste liquid collection component connected to the outlet.

[0033] At this time, when emptying the liquid storage assembly 10, the processor 30 is used to control the first control valve 23 to connect the liquid storage assembly 10 and the temporary storage mechanism 21, and first control the pressure supply mechanism 22 to switch the temporary storage mechanism 21 to a positive pressure state, and discharge it directly to the liquid collection assembly 40 through the outlet. However, the liquid at the bottom of the liquid storage assembly 10 cannot be discharged directly, so the processor 30 is further used to control the first control valve 23 to connect the liquid storage assembly 10 and the temporary storage mechanism 21, and control the pressure supply mechanism 22 to switch the temporary storage mechanism 21 to a negative pressure state, so that the liquid remaining in the liquid storage assembly 10 is attracted by the negative pressure in the pipeline and discharged through the pipeline between the inlet, the first control valve 23 and the liquid storage assembly 10.

[0034] In a possible embodiment, the temporary storage mechanism 21 includes a power pump 211, the pressure supply mechanism 22 includes a fifth control valve 221 and a first pressure source 222, the first pressure source 222 includes a positive pressure source 2221 and a negative pressure source 2222, a first end of the fifth control valve 221 is connected to the positive pressure source 2221 via a pipeline, a second end of the fifth control valve 221 is connected to the negative pressure source 2222 via a pipeline, and a third end of the fifth control valve 221 is connected to the power pump 211 via a pipeline. In this case, the processor 30 is configured to control the fifth control valve 221 to connect the positive pressure source 2221 and the power pump 211, thereby switching the temporary storage mechanism 21 to a positive pressure state; the processor 30 is also configured to control the fifth control valve 221 to connect the negative pressure source 2222 and the power pump 211, thereby switching the temporary storage mechanism 21 to a negative pressure state.

[0035] Therefore, the hematology analyzer of this embodiment controls the first control valve 23 through the processor 30 to connect the liquid storage assembly 10 and the temporary storage mechanism 21, and first controls the pressure supply mechanism 22 to switch the temporary storage mechanism 21 to a positive pressure state, allowing the liquid to be discharged through the outlet to the liquid collection assembly 40. The first control valve 23 is then controlled to connect the liquid storage assembly 10 and the temporary storage mechanism 21, and the pressure supply mechanism 22 is controlled to switch the temporary storage mechanism 21 to a negative pressure state, allowing the liquid to be discharged through the pipeline between the inlet, the first control valve 23, and the liquid storage assembly 10. Therefore, the liquid at the bottom of the liquid storage chamber 11 is ensured to be discharged smoothly, the drainage efficiency is improved, and the residual liquid in the liquid storage chamber 11 is reduced. Simultaneously, the operation is simple, and while ensuring the stability of the system operation, blockage caused by reagent evaporation and crystallization is reduced, thereby improving the service life and reliability of the hematology analyzer.

[0036] In some embodiments, the blood cell analyzer includes a reagent supply assembly 50, the temporary storage mechanism 21 includes a power pump 211, the first end of the first control valve 23 is connected to the power pump 211 through a pipeline, the second end of the first control valve 23 is connected to the reagent supply assembly 50 through a pipeline, and the third end of the first control valve 23 is connected to the liquid storage assembly 10 through a pipeline.

[0037] The processor 30 is used to: in response to receiving a discharge instruction, control the pressure supply mechanism 22 to switch the power pump 211 to a negative pressure state; control the first control valve 23 to connect the power pump 211 and the liquid storage component 10, so that the power pump 211 absorbs the liquid in the pipeline between the liquid storage cavity 11 and the first control valve 23; control the pressure supply mechanism 22 to switch the power pump 211 to a positive pressure state, and control the first control valve 23 to connect the power pump 211 and the reagent supply component 50, so that the power pump 211 discharges the absorbed liquid to the reagent supply component 50.

[0038] Specifically, the drain instruction received by the processor 30 may be an operation instruction generated when performing a liquid line drain operation of the hematology analyzer. Upon receiving the drain instruction, the processor 30 is configured to control the pressure supply mechanism 22 to switch the power pump 211 to a negative pressure state, and control the first control valve 23 to connect the power pump 211 and the liquid storage assembly 10, so that the power pump 211 draws liquid from the pipeline between the liquid storage cavity 11 and the first control valve 23. After the power pump 211 draws a sufficient amount of liquid from the liquid storage assembly 10, the processor 30 is further configured to control the pressure supply mechanism 22 to switch the power pump 211 to a positive pressure state, and control the first control valve 23 to connect the power pump 211 and the reagent supply assembly 50, so that the power pump 211 discharges the drawn liquid into the reagent supply assembly 50. After the absorbed liquid is discharged into the reagent supply assembly 50, the processor 30 is further configured to return to executing the steps of controlling the pressure supply mechanism 22 to switch the power pump 211 to a negative pressure state, controlling the first control valve 23 to connect the power pump 211 and the liquid storage assembly 10, and executing subsequent steps, so as to repeat the operation multiple times until the liquid in the liquid storage cavity 11 is emptied. In a possible embodiment, the liquid suction volume of the power pump 211 is fixed. In this case, the power pump 211 can be, but is not limited to, a fixed-flow pump.

[0039] Therefore, the blood cell analyzer of this embodiment can control the power pump 211 to switch between negative pressure and positive pressure states through the processor 30, so that the power pump 211 sucks out the liquid in the liquid storage cavity 11 and discharges the sucked liquid to the reagent supply assembly 50. The operation method is simple, reduces the complexity of the liquid pipeline and reduces the maintenance cost, thereby improving the service life and reliability of the blood cell analyzer.

[0040] In some embodiments, as Figure 2As shown, the reagent supply assembly 50 is used to provide a first reagent; before the power pump 211 draws the liquid from the pipeline between the liquid storage chamber 11 and the first control valve 23, the processor 30 is also used to: control the first control valve 23 to connect the power pump 211 and the reagent supply assembly 50, and control the pressure supply mechanism 22 to switch the temporary storage mechanism 21 to a negative pressure state, so that the power pump 211 draws the first reagent from the reagent supply assembly 50; control the first control valve 23 to connect the power pump 211 and the liquid storage assembly 10, and control the pressure supply mechanism 22 to switch the temporary storage mechanism 21 to a positive pressure state, so that the power pump 211 discharges the drawn first reagent into the liquid storage assembly 10 to clean the liquid storage assembly 10.

[0041] Specifically, the reagent supply assembly 50 is used to provide a first reagent to the liquid storage assembly 10. The first reagent can be, but is not limited to, pure water or deionized water. The reagent supply assembly 50 may include a first storage bottle 51 for storing the first reagent, which is connected to the first control valve 23 via a pipeline. Upon receiving a discharge instruction, the processor 30 controls the first control valve 23 to connect the power pump 211 and the liquid storage assembly 10, and controls the pressure supply mechanism 22 to switch the temporary storage mechanism 21 to a positive pressure state, thereby discharging the reagent liquid above the outlet of the liquid storage assembly 10 into the liquid collection assembly 40 (or incubation assembly 60). After the reagent above the outlet is emptied, the processor 30 further controls the first control valve 23 to connect the power pump 211 and the reagent supply assembly 50, and controls the pressure supply mechanism 22 to switch the temporary storage mechanism 21 to a negative pressure state, thereby allowing the power pump 211 to draw the first reagent from the reagent supply assembly 50. The processor 30 is also used to control the first control valve 23 to connect the power pump 211 and the liquid storage component 10, and control the pressure supply mechanism 22 to switch the temporary storage mechanism 21 to a positive pressure state, so that the power pump 211 discharges the absorbed first reagent to the liquid storage component 10, so that the first reagent can be used to clean the pipeline between the liquid storage component 10 and the first control valve 23 and dilute the liquid inside the liquid storage cavity 11.

[0042] After the cleaning is completed, for example, after the steps of the power pump 211 sucking the first reagent from the reagent supply assembly 50 and the power pump 211 discharging the sucked first reagent into the liquid storage assembly 10 are repeated multiple times, the processor 30 is further configured to execute the above-mentioned steps of controlling the pressure supply mechanism 22 to switch the power pump 211 to a negative pressure state; controlling the first control valve 23 to connect the power pump 211 and the liquid storage assembly 10 so that the power pump 211 sucks liquid from the pipeline between the liquid storage cavity 11 and the second control valve 213; and controlling the pressure supply mechanism 22 to switch the power pump 211 to a positive pressure state and controlling the first control valve 23 to connect the power pump 211 and the reagent supply assembly 50 so that the power pump 211 discharges the sucked liquid into the reagent supply assembly 50. In this way, all the liquid in the liquid storage assembly 10 is discharged into the reagent supply assembly 50.

[0043] Therefore, the blood cell analyzer of this embodiment uses the processor 30 to coordinately control the power pump 211, the first control valve 23 and the pressure supply mechanism 22, so that the liquid storage component 10 can be emptied while the pipeline between the first control valve 23 and the liquid storage component 10 is cleaned by the first reagent, which is beneficial to reducing the risk of crystallization of the liquid in the pipeline, reducing or avoiding blockage failures caused by reagent evaporation and crystallization, and further improving the service life and reliability of the blood cell analyzer.

[0044] Alternatively, see Figure 4 , Figure 4 FIG. 1 is a structural diagram of the fourth embodiment of the blood cell analyzer provided by the present application. Figure 4 As shown, the blood cell analyzer further includes an incubation component 60 . The reagent supply component 50 is used to provide a second reagent. The incubation component 60 is connected to the outlet of the liquid storage component 10 .

[0045] In one possible embodiment, before the power pump 211 draws liquid from the pipeline between the liquid storage chamber 11 and the first control valve 23, the processor 30 is used to control the power pump 211 to draw the second reagent from the reagent supply assembly 50 when the first control valve 23 connects the power pump 211 and the reagent supply assembly 50 and the power pump 211 is in a negative pressure state; and when the first control valve 23 connects the power pump 211 and the liquid storage assembly 10 and the power pump 211 is in a positive pressure state, the processor 30 is used to control the power pump 211 to discharge the drawn second reagent into the liquid storage assembly 10, so as to replenish the second reagent into the liquid storage assembly 10.

[0046] Specifically, the incubation assembly 60 is the aforementioned liquid collection assembly 40, and the reagent supply assembly 50 includes a second storage bottle 52 for storing a second reagent, and the second storage bottle 52 is connected to the first control valve 23 via a pipeline. Therefore, during the normal operation process of the hematology analyzer, the processor 30 is used to control the first control valve 23 to connect the power pump 211 and the reagent supply assembly 50, and control the power supply mechanism to switch the power pump 211 to a negative pressure state, so that the power pump 211 draws the second reagent from the reagent supply assembly 50 under the action of the negative pressure. The processor 30 is also used to control the first control valve 23 to connect the power pump 211 and the liquid storage assembly 10, and control the power supply mechanism to switch the power pump 211 to a positive pressure state, so that the power pump 211, under the action of the positive pressure, discharges the drawn second reagent through the pipeline and enters the liquid storage assembly 10 through the inlet. At this time, the liquid storage component 10 can be used as a temporary storage component for the second reagent, or the liquid storage component 10 also includes a heating mechanism arranged in the liquid storage cavity 11, and the heating mechanism is used to heat the second reagent in the liquid storage cavity 11 to achieve preheating of the second reagent.

[0047] In another possible embodiment, before the power pump 211 draws the liquid from the pipeline between the liquid storage chamber 11 and the first control valve 23, the processor 30 is used to control the first control valve 23 to connect the liquid storage component 10 and the power pump 211, and control the pressure supply mechanism 22 to switch the power pump 211 to a positive pressure state so that the liquid in the liquid storage chamber 11 is discharged to the incubation component 60 through the outlet.

[0048] Specifically, when the incubation assembly 60 needs to use the preheated second reagent, a sufficient amount of the second reagent is stored in the liquid storage chamber 11. The processor 30 is used to control the first control valve 23 to connect the liquid storage assembly 10 and the power pump 211, and control the pressure supply mechanism 22 to switch the power pump 211 to a positive pressure state, so that the second reagent in the liquid storage chamber 11 is discharged through the outlet to the incubation assembly 60 under the action of positive pressure.

[0049] Alternatively, in response to receiving the drain instruction, the processor 30 is configured to first disconnect the reagent supply assembly 50 from the power pump 211, so that the power pump 211 is connected to the external air source through the second end of the first control valve 23. In other words, the storage bottle of the reagent supply assembly 50 is removed, so that the power pump 211 is connected to the external air through the first control valve 23. The processor 30 is also used to first control the first control valve 23 to connect the external air source and the power pump 211, and control the pressure supply mechanism 22 to switch the power pump 211 to a negative pressure state, so that the power pump 211 can suck the reagent and / or external air from the pipeline between the first control valve 23 and the external air source; and then control the first control valve 23 to connect the liquid storage component 10 and the power pump 211, and control the pressure supply mechanism 22 to switch the power pump 211 to a positive pressure state, so that the power pump 211 can pump the absorbed gas, liquid and / or liquid between the first control valve 23 and the liquid storage component 10 into the liquid storage component 10, and discharge the liquid in the liquid storage component 10 to the incubation component 60 through the outlet. At this time, the processor 30 can be used to repeatedly execute the steps of controlling the first control valve 23 to connect the external air source and the power pump 211, and controlling the pressure supply mechanism 22 to switch the power pump 211 to a negative pressure state, and controlling the first control valve 23 to connect the liquid storage component 10 and the power pump 211, and controlling the pressure supply mechanism 22 to switch the power pump 211 to a positive pressure state, until the liquid in the liquid storage component is completely discharged to the incubation component 60 through the outlet. The complete discharge here can be understood as most of it being discharged, and a small amount of liquid remains at the bottom or side wall due to the contact between the liquid and the liquid storage component.

[0050] Therefore, the blood cell analyzer of this embodiment can precisely control the power pump 211, the first control valve 23 and the pressure supply mechanism 22 through the processor 30 to achieve the absorption and discharge of the second reagent, thereby ensuring the normal use of the incubation component 60 while improving the efficiency and accuracy of the detection.

[0051] In a possible embodiment, when the liquid storage assembly 10 is used as a reagent preheating assembly, the first control valve 23 and the fifth control valve 221 can be, but are not limited to, three-way valves, or the first control valve 23 and the fifth control valve 221 can be other valve devices that can realize three-way channel control. In this case, when the fifth control valve 221 is not driven by the outside world, the fifth control valve 221 is used to connect the negative pressure source 2222 and the power pump 211; when the processor 30 controls the fifth control valve 221 to open, the fifth control valve 221 is used to connect the positive pressure source 2221 and the power pump 211. When the first control valve 23 is not driven by the outside world, the first control valve 23 is used to connect the reagent supply assembly 50 and the power pump 211; when the processor 30 controls the first control valve 23 to open, the first control valve 23 is used to connect the liquid storage assembly 10 and the power pump 211.

[0052] In some embodiments, the blood cell analyzer includes a reagent supply assembly 50, the temporary storage mechanism 21 includes a liquid storage tank 212, a power pump 211 and a second control valve 213, the power pump 211 is connected to the liquid storage tank 212 through the second control valve 213, the liquid storage tank 212 is connected to the liquid storage assembly 10 through the first control valve 23, and the pressure supply mechanism 22 is respectively connected to the liquid storage tank 212 and the power pump 211.

[0053] Specifically, in this embodiment, the liquid stored in the liquid storage cavity 11 of the liquid storage assembly 10 is sheath fluid, which is used to assist the liquid storage assembly 10 in sample sheath flow detection. Alternatively, the liquid in the liquid storage cavity 11 is a mixed liquid of any combination of sheath fluid, sample solution, diluent, cleaning fluid, detection reagent, etc. In this case, the liquid storage tank 212 is used to store the sheath fluid reagent. During the sheath flow detection process, the pressure supply mechanism 22 is used to provide a positive pressure environment for the liquid storage tank 212, so that the sheath fluid reagent in the liquid storage tank 212 enters the liquid storage assembly 10 for use under the action of positive pressure through the pipeline between the liquid storage tank 212 and the first control valve 23.

[0054] Among them, the processor 30 is used to: in response to receiving a discharge instruction, control the pressure supply mechanism 22 to switch the power pump 211 to a negative pressure state, and control the second control valve 213 to connect the liquid storage tank 212 and the power pump 211, so that the power pump 211 absorbs the air in the liquid storage tank 212; when the air pressure in the liquid storage tank 212 reaches a preset condition, control the first control valve 23 to connect the liquid storage tank 212 and the liquid storage component 10, so that the liquid in the liquid storage cavity 11 is discharged to the liquid storage tank 212 through the pipeline between the inlet, the first control valve 23 and the liquid storage component 10.

[0055] Specifically, when the drainage instruction indicates that the liquid storage component 10 needs to perform a drainage operation, the processor 30 is used to control the pressure supply mechanism 22 to switch the power pump 211 to a negative pressure state, and control the second control valve 213 to connect the liquid storage tank 212 and the power pump 211, so that the power pump 211 absorbs the air in the liquid storage tank 212, and the air pressure in the liquid storage tank 212 decreases. When the air pressure in the liquid storage tank 212 reaches a preset condition, that is, when the pressure value in the liquid storage tank 212 reaches a preset negative pressure value, the processor 30 is also used to control the first control valve 23 to connect the liquid storage tank 212 and the liquid storage component 10, so that the pipeline between the liquid storage tank 212 and the liquid storage component 10 is in a negative pressure environment. Under the action of negative pressure, the liquid in the liquid storage cavity 11 is discharged into the liquid storage tank 212 through the inlet, the first control valve 23 and the pipeline between the liquid storage component 10, thereby realizing the emptying of the liquid storage cavity 11. It can be understood that the emptying here means to drain away all the residual liquid in the liquid storage cavity 11.

[0056] In a possible embodiment, the hematology analyzer further includes a reagent supply assembly 50 for providing a sheath reagent. For example, the reagent supply assembly 50 includes a third storage bottle 53, which is connected to the power pump 211 via the second control valve 213. In this case, the power pump 211 can also be used to draw the sheath reagent from the reagent supply assembly 50 and discharge the sheath reagent into the liquid storage tank 212, thereby replenishing the reagent in the liquid storage tank 212.

[0057] Therefore, upon receiving a discharge instruction, the hematology analyzer of this embodiment controls the pressure supply mechanism 22 via the processor 30 to switch the power pump 211 to a negative pressure state, and controls the second control valve 213 to connect the liquid storage tank 212 and the power pump 211, so that the power pump 211 draws air from the liquid storage tank 212 and increases the air pressure in the liquid storage tank 212 to a preset negative pressure value. The processor 30 is further configured to control the first control valve 23 to connect the liquid storage tank 212 and the liquid storage assembly 10, so that the liquid in the liquid storage chamber 11 is discharged into the liquid storage tank 212 through the inlet under the action of the negative pressure of the liquid storage tank 212, thereby clearing residual reagent from the liquid storage chamber 11, reducing or avoiding blockages caused by reagent evaporation and crystallization, and improving the service life and reliability of the hematology analyzer.

[0058] In some embodiments, the liquid circuit emptying assembly 20 also includes a third control valve 24 and a drainage branch 25, the first end of the third control valve 24 is connected to the pipeline between the liquid storage tank 212 and the first control valve 23, and the second end of the third control valve 24 is connected to the drainage branch 25; the processor 30 is also used to control the first control valve 23 to cut off and control the third control valve 24 to be connected, so that the liquid in the liquid storage tank 212 is discharged to the drainage branch 25.

[0059] Specifically, the third control valve 24 is used to control the flow of liquid in the pipeline between the liquid storage tank 212 and the first control valve 23. After the residual reagent in the liquid storage chamber 11 is drawn through the liquid storage tank 212, the processor 30 is used to control the first control valve 23 to be cut off, thereby blocking the pipeline connection between the liquid storage tank 212 and the liquid storage assembly 10. The processor 30 is also used to control the third control valve 24 to be opened, thereby opening the pipeline between the liquid storage tank 212 and the drainage branch 25, allowing the liquid in the liquid storage tank 212 to be discharged through the drainage branch 25 without affecting the normal operation of other fluid systems.

[0060] Therefore, this embodiment can achieve rapid emptying of the liquid in the liquid storage tank 212 through the control of the third control valve 24 and the first control valve 23 by the processor 30, preventing residual liquid from flowing back into the liquid storage component 10, and the drainage operation is simple and fast.

[0061] In some embodiments, the pressure supply mechanism 22 includes a first pressure source 222, a second pressure source 223 and a fourth control valve 224. The first pressure source 222 is connected to the power pump 211, and the second pressure source 223 is connected to the liquid storage tank 212 through the fourth control valve 224. The second pressure source 223 is used to provide positive pressure for the liquid storage tank 212. The blood cell analyzer also includes a liquid collection component 40, which is connected to the outlet of the liquid storage component 10.

[0062] Among them, before the liquid in the liquid storage chamber 11 is discharged to the liquid storage tank 212 through the pipeline between the inlet, the first control valve 23 and the liquid storage component 10, the processor 30 is used to control the first control valve 23 to connect the liquid storage component 10 and the liquid storage tank 212 in response to receiving the discharge instruction, and control the fourth control valve 224 to be connected, so that the liquid storage tank 212 discharges the liquid in the liquid storage chamber 11 through the outlet to the liquid collection component 40 under the positive pressure of the second pressure source 223.

[0063] Specifically, when the liquid storage assembly 10 needs to be emptied, the processor 30 is configured to control the first control valve 23 to connect the liquid storage assembly 10 and the liquid storage tank 212, and to control the fourth control valve 224 to connect, so that the liquid storage tank 212 discharges a portion of the liquid in the liquid storage cavity 11 into the liquid collection assembly 40 under the positive pressure of the second pressure source 223. The processor 30 is also configured to execute the steps of controlling the pressure supply mechanism 22 to switch the power pump 211 to a negative pressure state, and controlling the second control valve 213 to connect the liquid storage tank 212 and the power pump 211 so that the power pump 211 draws air from the liquid storage tank 212, and subsequent steps. Therefore, the processor 30 can flexibly respond to different liquid discharge requirements by controlling the fourth control valve 224 to achieve pressure control of the second pressure source 223 and driving and controlling the power pump 211 through the first pressure source 222, thereby ensuring the safety and reliability of the liquid discharge process.

[0064] Among them, before the liquid in the liquid storage chamber 11 is discharged to the liquid storage tank 212 through the pipeline between the inlet, the first control valve 23 and the liquid storage component 10, the processor 30 is also used to control the second control valve 213 to connect the power pump 211 and the liquid storage tank 212 in response to receiving a discharge instruction, and control the first pressure source 222 to switch the power pump 211 to a positive pressure state, so that the power pump 211 will discharge the absorbed reagent to the liquid storage component 10 to clean the liquid storage component 10.

[0065] In some embodiments, the liquid circuit emptying assembly 20 further includes a waste liquid storage mechanism 26. The first end of the second control valve 213 is connected to the power pump 211 via a pipeline, the second end of the second control valve 213 is connected to the liquid storage tank 212 via a pipeline, and the third end of the second control valve 213 is connected to the waste liquid storage mechanism 26 via a pipeline. The temporary storage mechanism 21 further includes a sixth control valve 27. The waste liquid storage mechanism 26 is connected to the pipeline between the second control valve 213 and the third storage bottle 53 via the sixth control valve 27.

[0066] The processor 30 is used to: in response to receiving a discharge instruction, first control the second pressure source 223 to switch the power pump 211 to a positive pressure state, and control the second control valve 213 to connect the power pump 211 and the waste liquid storage mechanism 26 to discharge the gas and / or liquid of the power pump 211 to the waste liquid storage mechanism 26; then control the second pressure source 223 to switch the power pump 211 to a negative pressure state, and control the second control valve 213 to connect the liquid storage tank 212 and the power pump 211, so that the power pump 211 absorbs air from the liquid storage tank 212; return to repeat the steps of controlling the second pressure source 223 to switch the power pump 211 to a positive pressure state, and controlling the second control valve 213 to connect the power pump 211 and the waste liquid storage mechanism 26, and controlling the second pressure source 223 to switch the power pump 211 to a negative pressure state, and controlling the second control valve 213 to connect the liquid storage tank 212 and the power pump 211, until the air pressure in the liquid storage tank 212 reaches the preset condition.

[0067] Specifically, when the drain instruction instructs to drain the liquid from the liquid storage chamber 11, the processor 30 is configured to control the first control valve 23 to connect the liquid storage assembly 10 and the liquid storage tank 212, and to control the fourth control valve 224 to connect, so that the liquid storage tank 212 discharges most of the liquid from the liquid storage chamber 11 into the liquid collection assembly 40 under the positive pressure of the second pressure source 223. The processor 30 is also configured to control the second pressure source 223 to switch the power pump 211 to a positive pressure state, control the second control valve 213 to connect the power pump 211 and the waste liquid storage mechanism 26, and control the sixth control valve 27 to connect, so that the liquid in the power pump 211 is discharged into the waste liquid storage mechanism 26 under the action of the positive pressure. The processor 30 is further configured to control the second pressure source 223 to switch the power pump 211 to a negative pressure state, and to control the second control valve 213 to connect the liquid storage tank 212 and the power pump 211, so that the power pump 211, under the action of the negative pressure, draws air from the liquid storage tank 212 and liquid remaining in the pipeline between the liquid storage tank 212 and the second control valve 213. As the power pump 211 draws air from the liquid storage tank 212, the air pressure in the liquid storage tank 212 decreases.

[0068] The processor 30 is also configured to repeatedly discharge the liquid within the power pump 211 into the waste liquid storage mechanism 26, and subsequently to cause the power pump 211 to draw air from the liquid storage tank 212. Thus, the air in the liquid storage tank 212 is drawn multiple times to gradually reduce the air pressure within the liquid storage tank 212 until the liquid storage tank 212 reaches a preset negative pressure value and the liquid storage tank 212 is placed in a negative pressure environment. The processor 30 is also configured to, when the air pressure in the liquid storage tank 212 reaches a preset condition, control the first control valve 23 to connect the liquid storage tank 212 and the liquid storage assembly 10, thereby allowing the liquid in the liquid storage chamber 11 to enter the liquid storage tank 212; and control the third control valve 24 to connect, thereby allowing the liquid in the liquid storage tank 212 to be discharged through the drainage branch 25.

[0069] Therefore, the blood cell analyzer of this embodiment can reduce the air pressure in the liquid storage tank 212 through multiple suction and discharge operations of the power pump 211 and allow the liquid in the liquid storage cavity 11 to be sucked out under the negative pressure environment of the liquid storage tank 212, so as to remove the residual reagent in the liquid storage cavity 11, reduce or avoid the blockage failure caused by the evaporation and crystallization of the reagent; the liquid and gas in the liquid storage tank 212 sucked by the power pump 211 can be discharged through the waste liquid storage mechanism 26, thereby reducing environmental pollution and improving the reliability of the blood cell analyzer.

[0070] In a possible embodiment, when the liquid storage assembly 10 is used as a sheath flow cell, the second control valve 213 and the fifth control valve 221 can be, but are not limited to, three-way valves. Alternatively, the second control valve 213 and the fifth control valve 221 can be other valve devices capable of controlling three fluid channels. In this case, when the fifth control valve 221 is not externally driven, the fifth control valve 221 is used to connect the negative pressure source 2222 and the power pump 211; when the processor 30 controls the fifth control valve 221 to open, the fifth control valve 221 is used to connect the positive pressure source 2221 and the power pump 211. When the second control valve 213 is not externally driven, the second control valve 213 is used to connect the third storage bottle 53 and the power pump 211; when the processor 30 controls the second control valve 213 to open, the second control valve 213 is used to connect the liquid storage tank 212 and the power pump 211. The first control valve 23 , the third control valve 24 , the fourth control valve 224 and the sixth control valve 27 are two-way valves, or other valve devices capable of controlling two fluid channels.

[0071] The present application also provides a blood cell analysis method, which is applied to the blood cell analyzer in any of the above embodiments. Figure 5 , Figure 5 This is a flow chart of an embodiment of the blood cell analysis method provided by this application. Figure 5 As shown, the blood cell analysis method includes: Step S10 : In response to receiving the drain instruction, the temporary storage mechanism 21 is switched to a positive pressure state, and the first control valve 23 is controlled to connect the liquid storage assembly 10 and the temporary storage mechanism 21 , driving the reagent into the liquid storage assembly 10 to clean the liquid storage assembly 10 .

[0072] Specifically, the temporary storage mechanism 21 is used to replenish the reagent of the liquid storage assembly 10. In a possible embodiment, the blood cell analyzer further includes a reagent supply assembly 50, which is used to provide a first reagent. The blood cell analysis method can control the temporary storage mechanism 21 to absorb the first reagent from the reagent supply assembly 50 and discharge the first reagent into the liquid storage assembly 10, so that the pipeline between the temporary storage mechanism 21 and the liquid storage assembly 10 and the liquid storage assembly 10 are cleaned by the first reagent.

[0073] Step S20 : ​​controlling the liquid in the liquid storage assembly 10 to be discharged from the outlet of the liquid storage assembly 10 .

[0074] Specifically, the first control valve 23 can be controlled to connect the liquid storage assembly 10 and the temporary storage mechanism 21 , and the temporary storage mechanism 21 is first switched to a positive pressure state to allow the residual liquid in the liquid storage assembly 10 to be discharged from the outlet of the liquid storage assembly 10 .

[0075] Step S30: Switch the temporary storage mechanism 21 to a negative pressure state, and control the first control valve 23 to connect the liquid storage assembly 10 and the temporary storage mechanism 21, so that the liquid in the liquid storage assembly 10 is discharged through the inlet of the liquid storage assembly 10 and the pipeline between the first control valve 23 and the liquid storage assembly 10.

[0076] Specifically, the temporary storage mechanism 21 is switched to a negative pressure state, and the first control valve 23 is controlled to connect the liquid storage component 10 and the temporary storage mechanism 21, so that the pipeline between the first control valve 23 and the liquid storage component 10 is in a negative pressure environment. The liquid in the liquid storage cavity 11 of the liquid storage component 10 enters the pipeline between the first control valve 23 and the liquid storage component 10 through the inlet under the action of negative pressure, and is discharged through the pipeline.

[0077] Therefore, the blood cell analysis method of this embodiment can efficiently discharge the liquid in the liquid storage cavity 11 by switching the temporary storage mechanism 21 to a negative pressure state and using the first control valve 23 to conduct the pipeline, thereby maintaining the cleanliness and efficiency of the system, removing residual reagents in the liquid storage cavity 11, reducing or avoiding blockage failures caused by reagent evaporation and crystallization, and improving the service life and reliability of the blood cell analyzer.

[0078] In one embodiment, in step S20, the blood cell analysis method includes: in response to receiving a liquid discharge instruction, controlling the pressure supply mechanism 22 to switch the temporary storage mechanism 21 to a negative pressure state. In step S30, the blood cell analysis method includes: controlling the first control valve 23 to connect the liquid storage assembly 10 and the temporary storage mechanism 21, and to discharge the liquid in the liquid storage chamber 11 through the pipeline between the first control valve 23 and the liquid storage assembly 10.

[0079] Optionally, step S20 includes: controlling the first control valve 23 to connect the liquid storage component 10 and the temporary storage mechanism 21, and controlling the pressure supply mechanism 22 to switch the temporary storage mechanism 21 to a positive pressure state, so that the liquid in the liquid storage cavity 11 is discharged to the liquid collection component 40 through the outlet.

[0080] In one embodiment, in step S30, the blood cell analysis method includes: first controlling the pressure supply mechanism 22 to switch the power pump 211 to a negative pressure state; controlling the first control valve 23 to connect the power pump 211 and the liquid storage assembly 10, so that the power pump 211 absorbs liquid from the pipeline between the liquid storage chamber 11 and the second control valve 213; and then controlling the pressure supply mechanism 22 to switch the power pump 211 to a positive pressure state, and controlling the first control valve 23 to connect the power pump 211 and the reagent supply assembly 50, so that the power pump 211 discharges the absorbed liquid to the reagent supply assembly 50.

[0081] In one embodiment, in step S30, the blood cell analysis method includes: first, controlling the second pressure source 223 to switch the power pump 211 to a positive pressure state, and controlling the second control valve 213 to connect the power pump 211 and the waste liquid storage mechanism 26 to discharge the liquid in the power pump 211 into the waste liquid storage mechanism 26; then controlling the second pressure source 223 to switch the power pump 211 to a negative pressure state, and controlling the second control valve 213 to connect the liquid storage tank 212 and the power pump 211, so that the power pump 211 absorbs air from the liquid storage tank 212; returning to repeat the steps of controlling the second pressure source 223 to switch the power pump 211 to a positive pressure state, controlling the second control valve 213 to connect the power pump 211 and the waste liquid storage mechanism 26, and controlling the second pressure source 223 to switch the power pump 211 to a negative pressure state, and controlling the second control valve 213 to connect the liquid storage tank 212 and the power pump 211, until the air pressure in the liquid storage tank 212 reaches a preset condition.

[0082] In step S30, the blood cell analysis method includes: when the air pressure in the liquid storage tank 212 reaches a preset condition, controlling the first control valve 23 to connect the liquid storage tank 212 and the liquid storage assembly 10, so that the liquid in the liquid storage cavity 11 enters the liquid storage tank 212; controlling the first control valve 23 to be cut off and controlling the third control valve 24 to be connected, so that the liquid in the liquid storage tank 212 is discharged to the drainage branch 25.

[0083] In the present application, the blood cell analysis method can be applied when the instrument is packaged at the factory, and can also be used for maintenance operations when the blood cell analyzer is not used for a long time. Specifically, when the blood cell analyzer leaves the factory, all the reagents inside the instrument will be emptied and cleaned with pure water, and then the cleaned pure water cleaning fluid will be emptied from the outlet, and finally the residual reagents in the liquid storage component 10 will be emptied through the inlet of the liquid storage component 10 with a bottom inlet and top outlet structure. When the instrument is installed and used at the client after a period of time after leaving the factory, it will avoid internal reagent crystallization and blockage, and the problem of poor unpacking will occur at the client. Exemplarily, when the blood cell analyzer is not used for a long time, according to the user's operation on the analyzer, the instrument will automatically start the emptying process of the analyzer, clean the liquid storage component 10 with cleaning fluid, and then empty the cleaning fluid from the outlet of the liquid storage component 10, and finally empty the residual reagents in the liquid storage component 10 through the inlet of the liquid storage component 10 with a bottom inlet and top outlet structure, to prevent problems caused by internal reagent crystallization and blockage when the customer uses it next time. For example, when the analyzer is exported to foreign regions, it will also be scanned at the customs to check whether there is a large amount of residual liquid inside the instrument. If there is liquid, it will affect the export of the instrument. The inside of the instrument is cleaned by the blood cell analysis method of the present application and the residual reagent in the liquid storage component 10 is emptied through the inlet of the liquid storage component 10 with a bottom-in and top-out structure to avoid residual reagents at the time of customs export, which does not meet the customs requirements for the export of in vitro diagnostic devices, thereby affecting the sales and use of the instrument.

[0084] The above description is only an implementation method of the present application and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the description and drawings of this application, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A blood cell analyzer, characterized in that: include: The liquid storage assembly includes a liquid storage cavity having an inlet and an outlet, wherein the height of the inlet is smaller than the height of the outlet; A liquid circuit emptying assembly includes a temporary storage mechanism, a pressure supply mechanism, and a first control valve, wherein a first end of the temporary storage mechanism is connected to the pressure supply mechanism via a pipeline, and a second end of the temporary storage mechanism is connected to the inlet of the liquid storage assembly via the first control valve; a processor connected to the liquid path emptying component; In which, the processor is used to control the pressure supply mechanism to switch the temporary storage mechanism to a negative pressure state in response to receiving a discharge instruction, and the processor is also used to control the first control valve to connect the liquid storage component and the temporary storage mechanism so that the liquid in the liquid storage cavity is discharged through the pipeline between the first control valve and the liquid storage component.

2. The blood cell analyzer according to claim 1, characterized in that The blood cell analyzer further includes a liquid collection component connected to the outlet of the liquid storage component; the processor is configured to: In response to receiving the liquid discharge instruction, controlling the first control valve to connect the liquid storage assembly and the temporary storage mechanism, and first controlling the pressure supply mechanism to switch the temporary storage mechanism to a positive pressure state, so that the liquid in the liquid storage chamber is discharged to the liquid collection assembly through the outlet; Then, the first control valve is controlled to connect the liquid storage assembly and the temporary storage mechanism, and the pressure supply mechanism is controlled to switch the temporary storage mechanism to a negative pressure state, so that the liquid in the liquid storage cavity is discharged through the pipeline between the inlet, the first control valve and the liquid storage assembly.

3. The blood cell analyzer according to claim 1, characterized in that The blood cell analyzer includes a reagent supply assembly, the temporary storage mechanism includes a power pump, a first end of the first control valve is connected to the power pump via the pipeline, a second end of the first control valve is connected to the reagent supply assembly via the pipeline, and a third end of the first control valve is connected to the liquid storage assembly via the pipeline; wherein the processor is configured to: In response to receiving the liquid discharge instruction, controlling the pressure supply mechanism to switch the power pump to the negative pressure state; Controlling the first control valve to conduct the power pump and the liquid storage assembly, so that the power pump absorbs the liquid in the pipeline between the liquid storage chamber and the first control valve; The pressure supply mechanism is controlled to switch the power pump to a positive pressure state, and the first control valve is controlled to connect the power pump and the reagent supply assembly so that the power pump discharges the sucked liquid to the reagent supply assembly.

4. The blood cell analyzer according to claim 3, characterized in that The reagent supply assembly is used to provide a first reagent; before the power pump draws liquid from the pipeline between the liquid storage chamber and the first control valve, the processor is further used to: Controlling the first control valve to conduct the power pump and the reagent supply assembly, and controlling the pressure supply mechanism to switch the temporary storage mechanism to a negative pressure state, so that the power pump absorbs the first reagent of the reagent supply assembly; The first control valve is controlled to connect the power pump and the liquid storage assembly, and the pressure supply mechanism is controlled to switch the temporary storage mechanism to the positive pressure state, so that the power pump discharges the absorbed first reagent into the liquid storage assembly to clean the liquid storage assembly.

5. The blood cell analyzer according to claim 3, characterized in that: The hematology analyzer further includes an incubation component, the reagent supply component is used to provide a second reagent, and the incubation component is connected to the outlet of the liquid storage component; before the power pump draws the liquid from the pipeline between the liquid storage chamber and the first control valve, the processor is further used to: When the first control valve connects the power pump and the reagent supply assembly and the temporary storage mechanism is in the negative pressure state, the power pump is controlled to absorb the second reagent of the reagent supply assembly; when the first control valve connects the power pump and the liquid storage assembly and the temporary storage mechanism is in the positive pressure state, the power pump is controlled to discharge the absorbed second reagent into the liquid storage assembly, so that the second reagent is replenished into the liquid storage assembly; and / or, The first control valve is controlled to connect the liquid storage component and the power pump, and the pressure supply mechanism is controlled to switch the power pump to the positive pressure state, so that the liquid in the liquid storage cavity is discharged to the incubation component through the outlet.

6. The blood cell analyzer according to claim 1, characterized in that: The blood cell analyzer includes a reagent supply assembly, the temporary storage mechanism includes a liquid storage tank, a power pump and a second control valve, the power pump is connected to the liquid storage tank through the second control valve, the liquid storage tank is connected to the liquid storage assembly through the first control valve, and the pressure supply mechanism is connected to the liquid storage tank and the power pump respectively; The processor is configured to: In response to receiving the discharge instruction, controlling the pressure supply mechanism to switch the power pump to the negative pressure state, and controlling the second control valve to connect the liquid storage tank and the power pump, so that the power pump draws air from the liquid storage tank; When the air pressure in the liquid storage tank reaches a preset condition, the first control valve is controlled to connect the liquid storage tank and the liquid storage assembly, so that the liquid in the liquid storage cavity is discharged to the liquid storage tank through the pipeline between the inlet, the first control valve and the liquid storage assembly.

7. The blood cell analyzer according to claim 6, characterized in that: The liquid drain assembly further includes a third control valve and a drain branch, wherein a first end of the third control valve is connected to the pipeline between the liquid storage tank and the first control valve, and a second end of the third control valve is connected to the drain branch; The processor is further configured to control the first control valve to be cut off and control the third control valve to be turned on, so that the liquid in the liquid storage tank is discharged to the liquid drain branch.

8. The blood cell analyzer according to claim 6, characterized in that: The pressure supply mechanism includes a first pressure source, a second pressure source, and a fourth control valve. The first pressure source is connected to the power pump, and the second pressure source is connected to the liquid storage tank through the fourth control valve. The second pressure source is used to provide positive pressure to the liquid storage tank. The blood cell analyzer also includes a liquid collection assembly, which is connected to the outlet of the liquid storage assembly. Wherein, before the liquid in the liquid storage cavity is discharged to the liquid storage tank through the pipeline between the inlet, the first control valve and the liquid storage assembly, the processor is used to control the first control valve to connect the liquid storage assembly and the liquid storage tank in response to receiving the liquid discharge instruction, and control the fourth control valve to connect, so that the liquid storage tank discharges the liquid in the liquid storage cavity through the outlet to the liquid collection assembly under the positive pressure of the second pressure source; Before the liquid in the liquid storage chamber is discharged to the liquid storage tank through the pipeline between the inlet, the first control valve and the liquid storage component, the processor is also used to control the second control valve to connect the power pump and the liquid storage tank in response to receiving the discharge instruction, and control the first pressure source to switch the power pump to a positive pressure state, so that the power pump can discharge the absorbed reagent into the liquid storage component to clean the liquid storage component.

9. The blood cell analyzer according to claim 8, characterized in that: The liquid circuit emptying assembly further includes a waste liquid storage mechanism, wherein a first end of the second control valve is connected to the power pump via the pipeline, a second end of the second control valve is connected to the liquid storage tank via the pipeline, and a third end of the second control valve is connected to the waste liquid storage mechanism via the pipeline; The processor is configured to: In response to receiving the discharge instruction, firstly controlling the second pressure source to switch the power pump to a positive pressure state, and controlling the second control valve to connect the power pump and the waste liquid storage mechanism, so as to discharge the liquid in the power pump into the waste liquid storage mechanism; Then, controlling the second pressure source to switch the power pump to the negative pressure state, and controlling the second control valve to connect the liquid storage tank and the power pump, so that the power pump absorbs air from the liquid storage tank; Return to repeatedly execute the steps of controlling the second pressure source to switch the power pump to the positive pressure state, and controlling the second control valve to connect the power pump and the waste liquid storage mechanism, and controlling the second pressure source to switch the power pump to the negative pressure state, and controlling the second control valve to connect the liquid storage tank and the power pump, until the air pressure in the liquid storage tank reaches the preset condition.

10. A blood cell analysis method, characterized in that: Applied to the blood cell analyzer according to any one of claims 1 to 9, the blood cell analysis method comprises: In response to receiving a liquid discharge instruction, the temporary storage mechanism is switched to a positive pressure state, and the first control valve is controlled to connect the liquid storage component and the temporary storage mechanism, so as to drive the reagent into the liquid storage component to clean the liquid storage component; Controlling the liquid in the liquid storage component to be discharged from the outlet of the liquid storage component; The temporary storage mechanism is switched to a negative pressure state, and the first control valve is controlled to connect the liquid storage assembly and the temporary storage mechanism, so that the liquid in the liquid storage assembly is discharged through the inlet of the liquid storage assembly and the pipeline between the first control valve and the liquid storage assembly.

Images