Electromagnetically-driven integrated valve microflow plate

Abstract

The invention provides an electromagnetically-driven integrated valve microflow plate, and belongs to the technical field of SPR integrated flow path control, and the microflow plate comprises a flow channel layer, a first sample flow channel, a second sample flow channel and a bottom through hole, the valve plate layer is made of an elastic membrane material and is attached to the flow channel layer, and a first microcavity of the valve plate layer communicates with the through hole and forms a deformable bottom wall; the electromagnetic valve layer fixes an electromagnetic valve through an electromagnetic valve fixing seat, and a valve element of the electromagnetic valve layer abuts against the bottom wall; during power failure, the bottom wall deforms away from the runner layer, so that the through holes are communicated, and the sample runner is conducted; during electrification, the valve element pushes the bottom wall to seal the through hole, a sample flow channel is blocked, and the effects of fast electromagnetic drive response and accurate control over on-off of the flow channel are achieved; the integrated pile-up valve avoids pollution of external pipelines, and SPR detection accuracy is improved; the elastic membrane sealing reduces the risk of liquid leakage, and is suitable for high-throughput sample analysis of the surface plasma resonance detector.

Description

Technical Field

[0001] This application is a divisional application of patent number: 202110642857.3, with an application date of June 9, 2021. The present invention relates to the field of surface plasmon resonance detection technology, and specifically to an electromagnetically driven integrated valve microfluidic plate. Background Art

[0002] Surface Plasmon Resonance (SPR) is a novel biosensing and analytical technology. This technology uses the binding and dissociation curves of molecular complexes occurring on the sensor surface to monitor each step of the molecular binding process in real time. During testing, the sample flows through the flow channel on the flow plate to a designated location.

[0003] In the prior art, the flow path plate uses polymethyl methacrylate as the base material and is divided into two parts, namely the flow path layer and the cover plate layer; the flow path layer is carved into the required flow channel on its surface using computer digital controlled precision machining technology; the cover plate layer is a flat plate; then the two layers are bonded together using a bonding process or laser welding process to form an internal flow channel.

[0004] However, since valves cannot be integrated inside the flow path plate, only control valves can be set at the end, and the flow path cannot be controlled in a timely and effective manner as expected, causing the sample to not flow according to the predetermined flow channel when passing through the flow path plate, which has a great impact on the test results. Summary of the Invention

[0005] Therefore, the technical problem to be solved by the present invention is to overcome the defect in the prior art that valves cannot be integrated inside the flow path plate, and only control valves can be set at the end, which makes it impossible to timely and effectively control the flow path as expected, resulting in the sample not flowing according to the predetermined flow channel when passing through the flow path plate, which has a great impact on the detection results, thereby providing an electromagnetically driven integrated valve microfluidic plate.

[0006] The present invention also provides an electromagnetically driven integrated valve microfluidic plate.

[0007] In order to solve the above technical problems, the present invention provides an electromagnetically driven integrated valve microfluidic plate, comprising:

[0008] A flow channel layer is provided with a first sample flow channel and a second sample flow channel spaced apart therefrom. A first through hole and a second through hole are provided on a lower surface of the flow channel layer; the first through hole is connected to the first sample flow channel, and the second through hole is connected to the second sample flow channel;

[0009] A valve layer is made of an elastic film material and is attached to the lower surface of the flow channel layer; a first microcavity is formed on a side of the valve layer close to the flow channel layer, the first microcavity is connected to the first through hole and the second through hole, and the valve layer forms the bottom wall of the first microcavity;

[0010] The electromagnetic valve layer is fixedly arranged on a side of the valve plate layer away from the flow channel layer;

[0011] A solenoid valve is mounted on the solenoid valve layer via a solenoid valve fixing seat;

[0012] The valve core of the solenoid valve abuts against the bottom wall of the first microcavity, and the valve core is configured as follows:

[0013] When the power is turned off, the bottom wall is separated from the bottom wall, so that the bottom wall is deformed away from the flow channel layer and the first through hole and the second through hole are connected;

[0014] When power is supplied, the bottom wall is pushed toward the flow channel layer so that the bottom wall seals the first through hole and the second through hole.

[0015] As a preferred solution, the elastic film material of the valve layer is polydimethylsiloxane film, silicone film or plastic film.

[0016] As a preferred solution, the flow channel layer includes a first layer, a second layer, a third layer and a fourth layer laminated in sequence:

[0017] The first layer is provided with a sample inlet connector and a sample outlet connector;

[0018] The second layer is provided with a first communicating hole connected to the sample inlet connector and a second communicating hole connected to the sample outlet connector;

[0019] The third layer is provided with the first sample flow channel and the second sample flow channel, the first sample flow channel is connected to the first communicating hole, and the second sample flow channel is connected to the second communicating hole;

[0020] The fourth layer is provided with the first through hole and the second through hole.

[0021] As a preferred solution, the solenoid valve fixing seat and the valve plate layer are integrally formed, and the solenoid valve fixing seat is provided with a positioning groove, and the valve core of the solenoid valve is embedded in the positioning groove.

[0022] The present invention also provides a surface plasmon resonance detector, comprising any one of the above-mentioned integrated valve microfluidic plates for sample delivery.

[0023] The technical solution of the present invention has the following advantages:

[0024] 1. The integrated valve microfluidic plate for sample transport provided by the present invention comprises: a flow channel layer and a valve plate layer; when the valve plate layer is away from the flow channel layer, a microcavity is formed between the flow channel layer and the valve plate layer, thereby connecting the first sample flow channel and the second sample flow channel, allowing the sample to be tested to flow between the integrated valve microfluidic plate and enter a connected state; at the same time, under the action of an external force, the valve plate layer adheres to the lower surface of the flow channel layer and covers the first through hole and the second through hole, sealing the first through hole and the second through hole, preventing the sample to be tested from flowing smoothly within the integrated valve microfluidic plate and entering a disconnected state;

[0025] By controlling the valve layer, the disconnection and connection of the entire integrated valve microfluidic plate can be achieved, thereby controlling the flow of the sample to be tested in the integrated valve microfluidic plate well, allowing it to circulate according to the predetermined flow channel, and ensuring the accuracy of the test results.

[0026] 2. The integrated valve microfluidic plate for sample transportation provided by the present invention allows pressurized fluid to circulate in the power transmission channel of the power transmission layer. Under the action of the fluid pressure, the bottom wall is deformed to achieve sealing or opening of the first through hole or the second through hole, thereby controlling the connection or disconnection of the integrated valve microfluidic plate.

[0027] 3. The integrated valve microfluidic plate for sample delivery provided by the present invention includes: a first layer, a second layer, a third layer and a fourth layer; by setting up multiple layers, the sample flow channel is set on the third layer; no laser engraving or bonding process is required, which reduces the processing difficulty, reduces the processing cost, and shortens the processing cycle. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the specific embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0029] Figure 1 Schematic diagram of the exploded structure of the integrated valve microfluidic plate for sample transportation of the present invention.

[0030] Figure 2 Schematic diagram of the internal structure of the flow channel layer of the present invention viewed from above.

[0031] Figure 3 Schematic diagram of the structure of the upper surface of the flow channel layer of the present invention.

[0032] Figure 4 This is a schematic diagram of the internal main structure of the flow channel layer of the present invention.

[0033] Figure 5 Schematic diagram of the structure of the flow channel layer in a connected state according to an embodiment of the present invention.

[0034] Figure 6 This is a schematic structural diagram of a flow channel layer in a disconnected state according to an embodiment of the present invention.

[0035] Figure 7 This is a schematic diagram of the internal structure of the integrated valve microfluidic plate with a power delivery layer of the present invention.

[0036] Figure 8 Schematic diagram of the exploded structure of the integrated valve microfluidic plate with the solenoid valve layer of the present invention.

[0037] Figure 9 It is a structural schematic diagram of the solenoid valve layer of the present invention.

[0038] Figure 10 Schematic diagram of the structure of the flow channel layer in the connected state of another embodiment of the present invention.

[0039] Figure 11 This is a structural schematic diagram of another embodiment of the flow channel layer of the present invention in a disconnected state.

[0040] Description of reference numerals:

[0041] 1. Flow channel layer; 2. Valve plate layer; 3. Power transmission layer; 4. First sample flow channel; 5. Second sample flow channel; 6. First through hole; 7. Second through hole; 8. Sample injection connector; 9. Sample output connector; 10. First microcavity; 11. Second microcavity; 12. Buffer microcavity; 13. Bottom wall; 14. Solenoid valve layer; 15. Solenoid valve fixing seat; 16. Solenoid valve. DETAILED DESCRIPTION

[0042] The technical solution of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0043] In the description of the present invention, it should be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside", etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, they cannot be understood as limiting the present invention.

[0044] In the description of the present invention, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they may refer to fixed, detachable, or integral connections; mechanical or electrical connections; direct or indirect connections through an intermediate medium; and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on the specific circumstances.

[0045] In addition, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0046] Example 1

[0047] The integrated valve microfluidic plate for sample delivery provided in this embodiment is as follows: Figure 1 As shown, it comprises: a flow channel layer 1 and a valve plate layer 2; the flow channel layer comprises a first layer, a second layer, a third layer and a fourth layer which are sequentially laminated;

[0048] like Figure 2 、 3 As shown, a sample injection connector 8 and a sample outlet connector 9 are provided on the upper surface of the first layer; at the same time, a first communicating hole and a second communicating hole are provided on the first layer and the second layer respectively; the first communicating hole is connected to the sample injection connector, and the second communicating hole is connected to the sample outlet connector;

[0049] like Figure 4 As shown, a first sample flow channel and a second sample flow channel are spaced apart on the third layer; one end of the first sample flow channel is connected to the first communicating hole; one end of the second sample flow channel is connected to the second communicating hole;

[0050] A first through hole and a second through hole are spaced apart on the fourth layer, the other end of the first sample flow channel is connected to the first through hole, and the other end of the second sample flow channel is connected to the second through hole;

[0051] like Figure 4 As shown, a valve layer 2 is bonded to the lower surface of the flow channel layer 1, and the valve layer 2 is made of an elastic membrane material. Specifically, the elastic membrane material can be a polydimethylsiloxane membrane, a silicone membrane or a plastic film; a first microcavity 10 is provided on a side of the valve layer 2 close to the flow channel layer 1, and the first microcavity 10 is connected to the first through hole 6 and the second through hole 7; an external force is applied to the bottom wall 13 of the first microcavity 10, and the bottom wall 13 is deformed under the action of the external force, and its bottom wall 13 can be bonded to the lower surface of the flow channel layer 1 to seal the first through hole 6 and the second through hole 7.

[0052] Specifically, such as Figure 4As shown, the valve layer 2 and the flow channel layer 1 are in the initial setting state and have not been deformed;

[0053] like Figure 5 As shown, the sample fluid to be tested flows in the direction of the arrow; specifically, the sample to be tested enters the interior of the first sample flow channel 4 through the sample inlet connector 8, passes through the first sample flow channel 4, and then enters the interior of the first microcavity 10 through the first through-hole 6; after being filled with the sample fluid to be tested, the bottom wall 13 of the first microcavity 10 is deformed toward the side away from the flow channel layer 1 under the action of the sample fluid to be tested, becoming an arc, forming a channel connecting the first through-hole 6 and the second through-hole 7, and then the fluid to be tested enters the second sample flow channel 5 through the second through-hole 7, and finally flows out of the flow plate through the sample outlet connector 9;

[0054] like Figure 6 As shown, an external force is applied to the outer side of the bottom wall 13 of the first microcavity 10 in the direction of the arrow, and the bottom wall 13 is deformed toward the flow channel layer 1, covering the first through hole 6 and the second through hole 7, so that the sample to be tested in the first sample flow channel 4 cannot flow into the second sample flow channel 5, thereby blocking the sample to be tested, which is equivalent to providing a valve between the first sample flow channel 4 and the second sample flow channel 5.

[0055] like Figure 7 As shown, a second microcavity 11 is provided on a side of the valve layer 2 away from the flow channel layer 1, and the first microcavity 10 and the second microcavity 11 are provided correspondingly, that is, the first microcavity 10 and the second microcavity 11 share a bottom wall 13; a power transmission layer 3 is provided on the side of the valve layer 2 having the second microcavity 11, and a power transmission channel is provided in the power transmission layer 3. A buffer microcavity 12 is provided at one end of the power transmission channel, and a fluid connector is provided at the other end; the buffer microcavity 12 is connected to the second microcavity 11;

[0056] During use, pressurized fluid is transported from the fluid connector to the power transmission layer 3. The fluid can be either gas or liquid. The fluid enters the buffer microcavity 12 and the second microcavity 11. Due to the pressure of the fluid, under the action of the fluid, the bottom wall 13 of the second microcavity 11 is deformed toward the flow channel layer 1, and the cover is set on the first through hole 6 and the second through hole 7, so that the sample to be tested in the first sample flow channel 4 cannot flow into the second sample flow channel 5. The greater the pressure of the fluid, the tighter the closure.

[0057] As an alternative embodiment, Figure 8 、 9As shown, a solenoid valve layer 14 is provided on the side of the valve layer 2 away from the flow channel layer 1. The solenoid valve layer 14 has a solenoid valve 16. The solenoid valve 16 is fixed on the solenoid valve fixing seat 15 and assembled with screws. The valve core of the solenoid valve covers the bottom wall 13 of the first microcavity 10. The rest of the solenoid valve fixing seat 15 is fixed to the flow channel layer 1 by compacting the valve layer 2.

[0058] like Figure 10 As shown, when there is no external force, the sample to be tested flows normally in the flow channel layer 1;

[0059] like Figure 11 As shown, the valve core pushes up the bottom wall 13 of the first microcavity 10 toward the flow channel layer 1, and the bottom wall 13 of the first microcavity 10 is elastically deformed to close the first through hole 6 and the second through hole 7. At this time, the sample to be tested cannot flow normally in the first sample flow channel 4 and the second sample flow channel 5, and the entire integrated valve microfluidic plate is in a closed state.

[0060] Example 2

[0061] This embodiment provides a surface plasmon resonance detector, comprising the integrated valve microfluidic plate for sample delivery described in Example 1.

[0062] Obviously, the above embodiments are merely examples for clarity of explanation and are not intended to limit the implementation methods. Those skilled in the art will readily appreciate that other variations or modifications based on the above descriptions are possible. It is not necessary and impossible to enumerate all implementation methods here. Obvious variations or modifications arising therefrom remain within the scope of protection of the present invention.

Claims

1. An electromagnetically driven integrated valve microfluidic plate, characterized in that: include: A flow channel layer (1) is provided with a first sample flow channel (4) and a second sample flow channel (5) arranged at intervals therein, and a first through hole (6) and a second through hole (7) are provided on the lower surface of the flow channel layer (1); the first through hole (6) is communicated with the first sample flow channel (4), and the second through hole (7) is communicated with the second sample flow channel (5); A valve sheet layer (2) is made of an elastic film material and is attached to the lower surface of the flow channel layer (1); a first microcavity (10) is provided on a side of the valve sheet layer (2) close to the flow channel layer (1); the first microcavity (10) is connected to the first through hole (6) and the second through hole (7), and the valve sheet layer (2) forms a bottom wall (13) of the first microcavity (10); The electromagnetic valve layer (14) is fixedly arranged on a side of the valve plate layer (2) away from the flow channel layer (1); A solenoid valve (16) is mounted on the solenoid valve layer (14) via a solenoid valve fixing seat (15); The valve core of the solenoid valve (16) abuts against the bottom wall (13) of the first microcavity (10), and the valve core is configured as follows: When the power is turned off, the bottom wall (13) is separated, so that the bottom wall (13) is deformed away from the flow channel layer (1) and the first through hole (6) and the second through hole (7) are connected; When electricity is applied, the bottom wall (13) is pushed toward the flow channel layer (1), so that the bottom wall (13) seals the first through hole (6) and the second through hole (7).

2. The electromagnetically driven integrated valve microfluidic plate according to claim 1, characterized in that: The elastic film material of the valve layer (2) is polydimethylsiloxane film, silicone film or plastic film.

3. The electromagnetically driven integrated valve microfluidic plate according to claim 1, characterized in that: The flow channel layer (1) comprises a first layer, a second layer, a third layer and a fourth layer laminated in sequence: The first layer is provided with a sample inlet connector (8) and a sample outlet connector (9); The second layer is provided with a first communicating hole communicating with the sample inlet connector (8) and a second communicating hole communicating with the sample outlet connector (9); The third layer is provided with the first sample flow channel (4) and the second sample flow channel (5), the first sample flow channel (4) is connected to the first communication hole, and the second sample flow channel (5) is connected to the second communication hole; The fourth layer is provided with the first through hole (6) and the second through hole (7).

4. The electromagnetically driven integrated valve microfluidic plate according to claim 1, characterized in that: The electromagnetic valve fixing seat (15) and the valve plate layer (2) are integrally formed, and the electromagnetic valve fixing seat (15) is provided with a positioning groove, and the valve core of the electromagnetic valve (16) is embedded in the positioning groove.

5. A surface plasmon resonance detector, characterized in that: A microfluidic plate comprising an electromagnetically driven integrated valve according to any one of claims 1 to 4.

Images