Arabinose preparation capable of maintaining healthy level of blood sugar and preparation method of arabinose preparation

Abstract

The arabinose preparation capable of maintaining the healthy level of blood sugar is composed of arabinose and mulberry leaf polyphenol, the arabinose and the mulberry leaf polyphenol are ultrasonically induced to form porous dendritic co-crystals at low temperature, and the finally obtained co-crystals are fast and uniform in release and low in hygroscopicity and do not obviously change after being stored for a long time; the stability is good.

Description

Technical Field

[0001] The present invention relates to the field of sweeteners, and in particular to an arabinose preparation for maintaining healthy blood sugar levels and a preparation method thereof. Background Art

[0002] Sugar substitutes, also known as sweeteners, mostly do not cause a rise in blood sugar or insulin secretion. Some even have no reaction with the human body and are excreted immediately after consumption. Sugar substitutes can be divided into two categories based on their source: natural sweeteners and synthetic sweeteners. Natural sweeteners include xylitol, stevia, mogroside, trehalose, and erythritol; artificial sweeteners generally include acesulfame potassium and aspartame. In the prior art, sugar substitutes such as arabinose are often compounded with other polysaccharides or plant extracts to create formulations that produce a sweet taste without raising blood sugar.

[0003] Mulberry leaf extract contains a variety of physiologically active substances such as mulberry leaf flavonoids, mulberry leaf polyphenols, mulberry leaf polysaccharides, mulberry leaf alkaloids (1-DNJ), etc., which can block the absorption of carbohydrates in food to promote healthy blood sugar and reduce the absorption of carbohydrates.

[0004] L-arabinose is widely present in plant cell walls and is a component of biopolymers such as hemicellulose, gum and pectin. L-arabinose non-competitively inhibits the activity of intestinal sucrase, thereby delaying the breakdown of sucrose, slowing glucose absorption and thus delaying blood sugar and insulin responses, thereby achieving the purpose of controlling postprandial blood sugar. However, the intestinal release rate of arabinose is not high enough and the onset time is relatively long.

[0005] Existing technologies show that polysaccharides or plant extracts often cannot be well combined with sugar substitutes, and there are problems such as arabinose easily absorbing moisture and agglomerating, polysaccharides easily being degraded by gastric acid, the release speed in the intestine is not fast enough, and the release of sugar substitutes and plant extracts is not synchronized. Summary of the Invention

[0006] In order to solve the technical problems existing in the background technology, the present invention proposes an arabinose preparation for maintaining healthy blood sugar levels, comprising L-arabinose and mulberry leaf polyphenols.

[0007] The mass ratio of the L-arabinose to the mulberry leaf polyphenols is 2.5-4:1.

[0008] The preparation is a porous dendritic crystal powder.

[0009] The present invention also provides a method for preparing the above-mentioned arabinose preparation for maintaining healthy blood sugar levels, comprising the following steps:

[0010] S1, subjecting mulberry leaf extract powder to an extraction process to obtain a mulberry leaf extract concentrate;

[0011] S2. subjecting the mulberry leaf extract concentrate to alcohol precipitation to obtain mulberry leaf polyphenol powder;

[0012] S3. L-arabinose, mulberry leaf polyphenol powder and anhydrous ethanol are mixed, and then sonicated at low temperature and dried to obtain L-arabinose-polyphenol co-crystals.

[0013] In step S2, the alcohol precipitation temperature is 0°C, and the alcohol precipitation operation is repeated 2-5 times.

[0014] In step S3, the mass ratio of the L-arabinose, mulberry leaf polyphenol powder and anhydrous ethanol is 2.5-4:1:15-20.

[0015] In step S3, the parameters of the low-temperature ultrasound are as follows:

[0016] The ultrasonic frequency is 30-45kHz, the ultrasonic power is 200-300W, the cavitation intensity is 0.45-0.6MPa, the ultrasonic time is 100-150min, and the ultrasonic temperature is -15°C.

[0017] In step S3, the drying is freeze-drying for 24-48 hours.

[0018] The beneficial effects of the present invention are as follows: conventional low-temperature crystallization is only used for purification, but in the present invention, under -15°C conditions, ultrasound can induce the hydroxyl group (-OH) of L-arabinose to form multiple hydrogen bonds with the phenolic hydroxyl group and carbonyl group of mulberry leaf polyphenol to form a three-dimensional skeleton; ultrasound generates micron-sized cavitation bubbles in the solution, and the shear force caused by the collapse of the bubbles will directionally guide the crystals to grow along specific crystal planes to form a dendritic fractal structure, thereby forming porous dendritic crystals, thereby improving the release rate and storage stability of the preparation in the intestine. DETAILED DESCRIPTION

[0019] To facilitate understanding of the present invention, the present invention will be described more fully below in conjunction with specific embodiments. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to provide a more thorough and comprehensive understanding of the disclosure of the present invention.

[0020] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used in the specification of the present invention herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0021] The technical solution of the present invention is described more clearly and completely below with reference to specific embodiments and comparative examples.

[0022] Example 1

[0023] This example provides an arabinose preparation for maintaining healthy blood sugar levels, and its preparation method is as follows:

[0024] (1) Dissolve 30 g of mulberry leaf extract powder in 30 mL of purified water, add 70 mL of anhydrous ethanol, stir vigorously for 20 minutes, and then centrifuge. Take the supernatant and repeat the centrifugation until there is no white precipitate; collect all the supernatant and concentrate to 30 mL;

[0025] (2) 30 mL of the above concentrate was added dropwise with 100 mL of anhydrous ethanol under stirring, and then the temperature was lowered to 0°C to obtain a precipitate. The precipitate was then dissolved in distilled water and anhydrous ethanol was added dropwise to obtain a precipitate. The purification was repeated three times, and the final precipitate was dried to obtain mulberry leaf polyphenol powder;

[0026] (3) L-arabinose, mulberry leaf polyphenol powder and anhydrous ethanol were placed in a beaker at a mass ratio of 3:1:16, and the mixture was cooled to -15°C while being stirred. After the mixture was completely dissolved and stirred evenly, the beaker containing L-arabinose, mulberry leaf polyphenol powder and anhydrous ethanol was placed in an ultrasonic machine. After the ultrasonication, the reactants in the beaker were centrifuged at 3000 rpm for 10 minutes to obtain an arabinose-polyphenol eutectic precipitate. The L-arabinose-polyphenol eutectic precipitate was freeze-dried for 48 hours to obtain an L-arabinose-polyphenol eutectic powder. The ultrasonication time was 120 minutes, the ultrasonication frequency was 40 kHz, the ultrasonication power was 300 W, the cavitation intensity was 0.5 MPa, and the temperature of the reactants in the beaker was controlled to be -15°C during the ultrasonication process.

[0027] Example 2

[0028] This example provides an arabinose preparation for maintaining healthy blood sugar levels, and its preparation method is as follows:

[0029] (1) Dissolve 40 g of mulberry leaf extract powder in 50 mL of purified water, add 130 mL of anhydrous ethanol, stir vigorously for 30 minutes, and then centrifuge. Take the supernatant and repeat the centrifugation until there is no white precipitate; collect all the supernatant and concentrate to 40 mL;

[0030] (2) 40 mL of the above concentrate was added dropwise with 150 mL of anhydrous ethanol under stirring, and then cooled to 0°C to obtain a precipitate. The precipitate was then dissolved in distilled water and anhydrous ethanol was added dropwise to obtain a precipitate. The purification was repeated three times, and the final precipitate was dried to obtain mulberry leaf polyphenol powder;

[0031] (3) L-arabinose, mulberry leaf polyphenol powder and anhydrous ethanol were placed in a beaker at a mass ratio of 2.5:1:15, and the mixture was cooled to -15°C while being stirred. After the mixture was completely dissolved and stirred evenly, the beaker containing L-arabinose, mulberry leaf polyphenol powder and anhydrous ethanol was placed in an ultrasonic machine. After the ultrasonication, the reactants in the beaker were centrifuged at 3000 rpm for 10 minutes to obtain an arabinose-polyphenol eutectic precipitate. The L-arabinose-polyphenol eutectic precipitate was freeze-dried for 36 hours to obtain an L-arabinose-polyphenol eutectic powder. The ultrasonication time was 150 minutes, the ultrasonication frequency was 30 kHz, the ultrasonication power was 300 W, the cavitation intensity was 0.45 MPa, and the temperature of the reactants in the beaker was controlled at -15°C during the ultrasonication process.

[0032] Example 3

[0033] This example provides an arabinose preparation for maintaining healthy blood sugar levels, and its preparation method is as follows:

[0034] (1) Dissolve 30 g of mulberry leaf extract powder in 30 mL of purified water, add 70 mL of anhydrous ethanol, stir vigorously for 15 minutes, and then centrifuge. Take the supernatant and repeat the centrifugation until there is no white precipitate; collect all the supernatant and concentrate to 30 mL;

[0035] (2) 30 mL of the above concentrate was added dropwise with 100 mL of anhydrous ethanol under stirring, and then the temperature was lowered to 0°C to obtain a precipitate. The precipitate was then dissolved in distilled water and anhydrous ethanol was added dropwise to obtain a precipitate. The purification was repeated three times, and the final precipitate was dried to obtain mulberry leaf polyphenol powder;

[0036] (3) L-arabinose, mulberry leaf polyphenol powder and anhydrous ethanol were placed in a beaker at a mass ratio of 3.6:1:20, and the mixture was cooled to -15°C while being stirred. After the mixture was completely dissolved and stirred evenly, the beaker containing L-arabinose, mulberry leaf polyphenol powder and anhydrous ethanol was placed in an ultrasonic machine. After the ultrasonication, the reactants in the beaker were centrifuged at 3000 rpm for 10 minutes to obtain an arabinose-polyphenol eutectic precipitate. The L-arabinose-polyphenol eutectic precipitate was freeze-dried for 40 hours to obtain an L-arabinose-polyphenol eutectic powder. The ultrasonication time was 100 minutes, the ultrasonication frequency was 45 kHz, the ultrasonication power was 200 W, the cavitation intensity was 0.6 MPa, and the temperature of the reactants in the beaker was controlled at -15°C during the ultrasonication process.

[0037] Comparative Example 1

[0038] This comparative example proposes an arabinose preparation for maintaining healthy blood sugar levels. The preparation method is the same as that of Example 1, except that the ultrasonication step in step (3) is omitted.

[0039] Comparative Example 2

[0040] This comparative example proposes an arabinose preparation for maintaining healthy blood sugar levels. The preparation method is the same as that of Example 1, except that "the temperature of the reactants in the beaker is controlled to be -15°C during the ultrasonic process" is changed to "the temperature of the reactants in the beaker is controlled to be 25°C during the ultrasonic process."

[0041] Comparative Example 3

[0042] This comparative example proposes an arabinose preparation for maintaining healthy blood sugar levels. The preparation method is the same as that of Example 1, except that the "mulberry leaf polyphenols" in step (3) is replaced with "green tea polyphenols".

[0043] In Comparative Example 3, since mulberry leaf polyphenols contain 3 phenolic hydroxyl groups and green tea contains 8 phenolic hydroxyl groups, the excessive hydroxyl groups compete for hydrogen bonds, resulting in gelation of the gel solution and failure of co-crystallization.

[0044] Performance Testing

[0045] In vitro simulated release rate test of L-arabinose-polyphenol co-crystals:

[0046] The L-arabinose-polyphenol co-crystals were placed in simulated intestinal fluid, and the release rate of the L-arabinose-polyphenol co-crystals was measured and calculated after 30 minutes. The simulated intestinal fluid was prepared as follows: 3.2 g of KH2PO4 was dissolved in 250 mL of deionized water and the pH was adjusted to 6.8. 100 mL of 1% (w / v) pancreatic enzyme solution was added to the H2PO4 solution and the volume was adjusted to 500 mL. The pH was then adjusted to 6.8.

[0047] Moisture absorption test:

[0048] Equal amounts of the L-arabinose-polyphenol co-crystals prepared in Examples 1-3 and Comparative Examples 1-3 were tested for moisture absorption and weight gain according to the dessicator equilibrium method specified in the National Military Standard (GJB770A-97). The method involves placing a fixed amount of sample at a constant temperature and humidity (20°C and 75% RH) and measuring its weight at regular intervals. When the sample reaches equilibrium, the mass of water absorbed by the sample is measured to calculate the final moisture absorption and weight gain of the sample. Moisture absorption rate = (weight after moisture absorption - weight before moisture absorption) / weight before moisture absorption × 100%.

[0049] Accelerated testing:

[0050] The L-arabinose-polyphenol co-crystals prepared in Examples 1-3 and Comparative Examples 1-3 were subjected to accelerated test conditions (temperature 40°C ± 2°C, relative humidity 75% ± 5%) and long-term test conditions (temperature 25°C ± 2°C, relative humidity 60% ± 5%, 6 months) in accordance with the guidelines of the Chinese Pharmacopoeia "9001 Stability of Raw Materials and Preparations".

[0051] The above performance test data are shown in Table 1

[0052] Table 1 Performance test data of each embodiment and comparative example

[0053]

[0054] As shown in Table 1, low-temperature-induced co-crystallization can significantly improve the stability and intestinal release rate of each arabinose preparation, and effectively control the blood sugar level of the human body after eating.

[0055] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. An arabinose preparation for maintaining healthy blood sugar levels, characterized in that: Includes L-arabinose and mulberry leaf polyphenols.

2. The arabinose preparation for maintaining healthy blood sugar levels according to claim 1, characterized in that The mass ratio of the L-arabinose to the mulberry leaf polyphenols is 2.5-4:

1.

3. The arabinose preparation for maintaining healthy blood sugar levels according to claim 1 or 2, characterized in that: The preparation is a porous dendritic crystal powder.

4. A method for preparing the arabinose preparation for maintaining healthy blood sugar levels according to any one of claims 1 to 3, characterized in that: The steps include: S1, subjecting mulberry leaf extract powder to an extraction process to obtain a mulberry leaf extract concentrate; S2. subjecting the mulberry leaf extract concentrate to alcohol precipitation to obtain mulberry leaf polyphenol powder; S3. L-arabinose, mulberry leaf polyphenol powder and anhydrous ethanol are mixed, and then sonicated at low temperature and dried to obtain L-arabinose-polyphenol co-crystals.

5. The method for preparing the arabinose preparation for maintaining healthy blood sugar levels according to claim 4, characterized in that: In step S2, the alcohol precipitation temperature is 0°C, and the alcohol precipitation operation is repeated 2-5 times.

6. The method for preparing the arabinose preparation for maintaining healthy blood sugar levels according to claim 4 or 5, characterized in that: In step S3, the mass ratio of the L-arabinose, mulberry leaf polyphenol powder and anhydrous ethanol is 2.5-4:1:15-20.

7. The method for preparing the arabinose preparation for maintaining healthy blood sugar levels according to any one of claims 4 to 6, characterized in that: In step S3, the parameters of the low-temperature ultrasound are as follows: The ultrasonic frequency is 30-45kHz, the ultrasonic power is 200-300W, the cavitation intensity is 0.45-0.6MPa, the ultrasonic time is 100-150min, and the ultrasonic temperature is -15°C.

8. The method for preparing the arabinose preparation for maintaining healthy blood sugar levels according to any one of claims 4 to 7, characterized in that: In step S3, the drying is freeze-drying for 24-48 hours.

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