Applications of Aspergillus in the metabolism of flammable and explosive gases

Abstract

This invention belongs to the field of microbial technology, specifically relating to the application of Aspergillus in the metabolism of flammable and explosive gases. This invention discovers that Aspergillus S8 has excellent absorption and metabolic effects on flammable and explosive gases such as hydrogen and methane, as well as mixtures containing these gases. It can metabolize using flammable and explosive gases or mixtures containing them as a carbon source and / or energy source, thereby achieving highly efficient treatment of flammable and explosive gases and their mixtures. Furthermore, it offers high environmental safety, good removal efficiency, ease of use, and no toxic byproducts, making it convenient for application and conversion.

Description

Technical Field

[0001] This invention belongs to the field of microbial technology, specifically relating to the application of Aspergillus in the metabolism of flammable and explosive gases. Background Technology

[0002] Flammable and explosive gases are those that can form flammable mixtures with air, with an explosive range of 20% to 80%. Common flammable and explosive gases include hydrogen (H2), methane (CH4), and carbon monoxide (CO). Because flammable and explosive gases are prone to leakage and highly flammable, with low explosion limits, they pose an extremely high risk, capable of causing industrial accidents or fires and seriously threatening local ecosystems and human safety. Therefore, it is necessary to distinguish and classify these gases during production, transportation, storage, and use.

[0003] Studies have found that microorganisms play a crucial role in the decomposition and metabolism of flammable and explosive gases, thereby further regulating ecosystems. Furthermore, the process of using microorganisms to decompose and metabolize flammable and explosive gases is convenient, easy to operate, and has no toxic side effects.

[0004] Aspergillus ( Aspergillus It is a filamentous fungus that is widely distributed in grains, air, soil and various organic matter. It is an important species in the fermentation industry and food processing industry. Nearly 60 species have been utilized, mainly for brewing and vinegar making. However, its application in gas metabolism, especially in the metabolism of flammable and explosive gases, is relatively rare. Summary of the Invention

[0005] The purpose of this invention is to provide the application of Aspergillus in the metabolism of flammable and explosive gases. The Aspergillus S8 described in this invention has a good absorption and metabolism effect on flammable and explosive gases and mixed gases containing flammable and explosive gases, thereby increasing the biological applications of Aspergillus S8.

[0006] This invention provides the application of Aspergillus S8 in gas metabolism, wherein the gas includes hydrogen or a mixture of gases containing flammable and explosive gases; the flammable and explosive gases include hydrogen and / or methane; and the preservation number of Aspergillus S8 is CGMCC NO.40828.

[0007] The present invention also provides the application of an agent containing Aspergillus S8 in gas metabolism, wherein the gas includes hydrogen or a mixture of gases containing flammable and explosive gases; the flammable and explosive gases include hydrogen and / or methane; and the preservation number of Aspergillus S8 is CGMCC NO.40828.

[0008] Preferably, the microbial agent comprises a suspension of Aspergillus S8.

[0009] Preferably, the mixed gas includes one or more of the following four types:

[0010] 1) Hydrogen and carbon dioxide;

[0011] 2) Hydrogen and methane;

[0012] 3) Methane and carbon dioxide;

[0013] 4) Hydrogen, methane, and carbon dioxide.

[0014] Preferably, in the mixed gas 1), the volume ratio of hydrogen to carbon dioxide is (1~2):(1~2).

[0015] Preferably, in the mixed gas described in 2), the volume ratio of hydrogen to methane is (1~2):(1~2).

[0016] Preferably, in the mixed gas described in 3), the volume ratio of methane to carbon dioxide is (1~2):(1~2).

[0017] Preferably, in the mixed gas described in 4), the volume ratio of hydrogen, methane, and carbon dioxide is 1:1:1.

[0018] The present invention also provides a method for gas metabolism, wherein the gas is treated with Aspergillus S8 or an agent containing Aspergillus S8; the gas is hydrogen or a mixture of gases containing flammable and explosive gases; the flammable and explosive gases include hydrogen and / or methane; the preservation number of Aspergillus S8 is CGMCC NO.40828.

[0019] Preferably, the inoculant is a bacterial suspension of Aspergillus S8.

[0020] Beneficial effects:

[0021] This invention provides the application of Aspergillus S8 in gas metabolism, wherein the gas includes hydrogen or a mixture of gases containing flammable and explosive gases; the flammable and explosive gases include hydrogen and / or methane; and the preservation number of Aspergillus S8 is CGMCC NO.40828. This invention discovers that Aspergillus S8 has excellent absorption and metabolic effects on flammable and explosive gases such as hydrogen and methane, as well as mixtures containing flammable and explosive gases. It can metabolize using flammable and explosive gases or mixtures containing flammable and explosive gases as carbon sources and / or energy sources, thereby achieving efficient treatment of flammable and explosive gases and their mixtures. Furthermore, it exhibits high environmental safety, good removal effect, ease of use, and no toxic byproducts, making it convenient for application and conversion. Detailed Implementation

[0022] This invention provides the application of Aspergillus S8 in gas metabolism, wherein the gas includes hydrogen or a mixture of gases containing flammable and explosive gases; the flammable and explosive gases include hydrogen and / or methane; the accession number of Aspergillus S8 is CGMCC NO.40828. The Aspergillus S8 described in this invention has been disclosed in patent application number CN202311542973.3.

[0023] The present invention also provides the application of an agent containing Aspergillus S8 in gas metabolism, wherein the gas includes hydrogen or a mixture of gases containing flammable and explosive gases; the flammable and explosive gases include hydrogen and / or methane; and the preservation number of Aspergillus S8 is CGMCC NO.40828.

[0024] In one embodiment, the inoculant is a suspension of Aspergillus S8; in another embodiment, the effective viable cell concentration of the Aspergillus S8 suspension is 2% (vol / vol). In one embodiment, the resuspending agent of the Aspergillus S8 suspension is PBS phosphate buffer, wherein the PBS phosphate buffer is 1×PBS phosphate buffer with a pH of 6.8.

[0025] As one embodiment, the preparation method of the Aspergillus S8 bacterial suspension is as follows: Aspergillus S8 is inoculated into an inorganic salt culture medium and cultured by shaking to obtain Aspergillus S8 fermentation broth; the Aspergillus S8 fermentation broth is separated into solid and liquid components, and the precipitate is resuspended to obtain the Aspergillus S8 bacterial suspension. In one embodiment, the inorganic salt culture medium comprises 0.5 g / L KH₂PO₄, 0.5 g / L Na₂HPO₄, 0.4 g / L NaCl, 1.0 g / L KNO₃, 0.5 g / L NH₄Cl, 1.0 g / L MgSO₄·7H₂O, 0.2 g / L CaCl₂, 0.004 g / L FeSO₄·7H₂O, 0.004 g / L CuSO₄·5H₂O, 0.004 g / L MnSO₄·H₂O, 0.004 g / L ZnSO₄·7H₂O, and 0.00024 g / L NaMoO₄·2H₂O. In one embodiment, the shaking culture temperature is 28°C. In one embodiment, the shaking culture speed is 170 rpm. In one embodiment, the shaking culture time is 3 days.

[0026] In one embodiment, the mixed gas includes one or more of the following four types of mixed gas: 1) hydrogen and carbon dioxide; 2) hydrogen and methane; 3) methane and carbon dioxide; 4) hydrogen, methane and carbon dioxide.

[0027] In one embodiment, 1) the volume ratio of hydrogen to carbon dioxide in the mixed gas is (1~2):(1~2); in another embodiment, the volume ratio of hydrogen to carbon dioxide is 1:1, 1:2, or 2:1. In one embodiment, 2) the volume ratio of hydrogen to methane in the mixed gas is (1~2):(1~2); in another embodiment, the volume ratio of hydrogen to methane is 1:1, 1:2, or 2:1. In one embodiment, 3) the volume ratio of methane to carbon dioxide in the mixed gas is (1~2):(1~2); in another embodiment, the volume ratio of methane to carbon dioxide is 1:1, 1:2, or 2:1. In one embodiment, 4) the volume ratio of hydrogen, methane, and carbon dioxide in the mixed gas is 1:1:1.

[0028] The present invention also provides a method for gas metabolism, wherein the gas is treated with Aspergillus S8 or an agent containing Aspergillus S8; the gas is hydrogen or a mixture of gases containing flammable and explosive gases; the flammable and explosive gases include hydrogen and / or methane; the preservation number of Aspergillus S8 is CGMCC NO.40828.

[0029] In one embodiment, the inoculant is a suspension of Aspergillus S8; the relevant characteristics of the Aspergillus S8 suspension have been defined in the above technical solutions and will not be repeated here. In one embodiment, the volume of the Aspergillus S8 suspension is 2% of the gas volume.

[0030] To further illustrate the present invention, the technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0031] Unless otherwise specified, the experimental and detection methods in the following embodiments are conventional methods; unless otherwise specified, the reagents and materials can be purchased through conventional commercial channels.

[0032] Inorganic salt culture medium: KH2PO4 0.5 g / L, Na2HPO4 0.5 g / L, NaCl 0.4 g / L, KNO3 1.0 g / L, NH4Cl 0.5 g / L, MgSO4·7H2O 1.0 g / L, CaCl2 0.2 g / L, FeSO4·7H2O 0.004 g / L, CuSO4·5H2O 0.004 g / L, MnSO4·H2O 0.004 g / L, ZnSO4·7H2O 0.004 g / L, NaMoO4·2H2O 0.00024 g / L.

[0033] PDA medium: 200 g / L potato, 20 g / L glucose, 5 g / L peptone, 3 g / L potassium dihydrogen phosphate, 1.5 g / L magnesium sulfate, 20 g / L agar.

[0034] Example 1

[0035] This embodiment provides an experiment on the utilization effect of Aspergillus S8 on H2 gas, and the steps are as follows:

[0036] Add 50 mL of inorganic salt culture medium to a 250 mL sealed bottle. Replace the headspace gas in the sealed bottle with nitrogen, then add pure H2 gas. Inoculate each bottle with 2% of the isolated and purified Aspergillus S8 suspension. Add ambient air to the control group bottle as a control. After static incubation at 28℃ for 7 days, measure the following indicators:

[0037] The utilization rate of H2 by Aspergillus S8 was determined using a gas chromatography-mass spectrometry (GC2014C, purchased from Shimadzu Corporation). The test conditions were: TCD detector, inlet temperature 100℃, detector temperature 100℃, furnace temperature 90℃, carrier gas argon, and flow rate 30 mL / min. The results are shown in Table 1.

[0038] The average volume utilization rate was determined by the water displacement gas collection method, and the results are shown in Table 1.

[0039] When determining the dry weight of mycelia, the filter paper was first dried to constant weight, and the weight of the filter paper was accurately weighed. After thoroughly mixing the Aspergillus S8 fermentation broth, 30 mL was accurately measured and filtered. The filter residue was washed several times with distilled water until the filtrate was colorless. Then, it was placed in a 55℃ constant temperature drying oven and dried to constant weight. The total mass of the filter residue and filter paper was accurately weighed. The formula for calculating the dry weight of mycelia is: Dry weight of mycelia (mg) = Total mass of filter residue and filter paper (mg) - Mass of filter paper (mg). The test results are shown in Table 1.

[0040] Table 1. Utilization effect of Aspergillus S8 on H2 gas

[0041]

[0042] As shown in Table 1, the utilization rate of ambient air gas by adding Aspergillus S8 to the sealed bottle was only 2.016%, the volume utilization rate was 2.437%, and the mycelial dry weight content was 28.178 mg. However, when Aspergillus S8 was added to pure H2 gas, its H2 gas utilization capacity was significantly improved. This strain could efficiently utilize H2 gas as a nutrient source for its growth and reproduction. The utilization rate of H2 gas by this strain was 95.721%, the volume utilization rate was 51.110%, and the mycelial dry weight was 41.833 mg. The gas utilization rate of Aspergillus S8 in pure H2 gas was 47.480 times that of the control group, the volume utilization rate was 20.972 times that of the control group, and the mycelial dry weight was 1.730 times that of the control group. This indicates that the Aspergillus S8 provided by this invention has a better utilization effect on H2 gas, with a strong H2 gas utilization rate, and can utilize H2 gas as a nutrient source for growth and reproduction.

[0043] Example 2

[0044] This embodiment provides an experiment on the utilization effect of Aspergillus S8 on a mixture of CO2 and H2 gases. The steps are as follows:

[0045] Add 50 mL of inorganic salt culture medium to a 250 mL sealed bottle. Replace the headspace gas in the bottle with nitrogen. Add pure CO2 and H2 gases in three volume ratios: CH1 (CO2:H2 = 1:2), CH2 (CO2:H2 = 1:1), and CH3 (CO2:H2 = 2:1). Re-add the same volume ratio of mixed gases to the sealed bottle every 7 days, with each cycle lasting 7 days and repeated 4 times. Inoculate each bottle with 2% of a purified Aspergillus S8 suspension. Add ambient air to the control group bottle as a control.

[0046] After static incubation at 28℃ for 7 days, the average utilization rate of Aspergillus S8 for a mixture of CO2 and H2 gas over four cycles was determined using a gas chromatography-mass spectrometry (GC2014C, purchased from Shimadzu Corporation) system (i.e., one measurement per cycle, and the average value was taken, the same as in the following examples). The results are shown in Table 2. The test conditions were the same as in Example 1. The volume average utilization rate over four cycles was determined by the water displacement gas collection method (i.e., one measurement per cycle, and the average value was taken, the same as in the following examples), and the results are shown in Table 2. The determination of the mycelial dry weight in the fourth cycle was the same as in Example 1, and the results are shown in Table 2.

[0047] Table 2. Utilization efficiency of Aspergillus S8 on CO2 and H2 mixed gas over 4 cycles.

[0048]

[0049] Table 2 shows that after adding Aspergillus S8 to the sealed bottle, the utilization rate of ambient air gas was only 3.124%, the volume utilization rate was 3.560%, and the mycelial dry weight was 30.561 mg. However, when Aspergillus S8 was added to a mixture of pure CO2 and H2 gas, the CH3 group showed the most significant utilization, followed by the CH2 group and then the CH1 group. This strain can efficiently utilize the mixture of pure CO2 and H2 gas as a carbon source and energy source for its growth and reproduction. The CH3 group showed a utilization rate of 96.524% and a volume utilization rate of 63.404% for the CO2 and H2 mixture, with a mycelial dry weight of 48.03 mg. In the pure CO2 and H2 mixture, the gas utilization rate of Aspergillus S8 was 30.897 times that of the control group, the volume utilization rate was 17.810 times that of the control group, and the mycelial dry weight was 1.571 times that of the control group. This indicates that the Aspergillus S8 provided by the present invention has a good utilization effect on the mixed gas of pure CO2 and H2, and has a strong utilization rate of pure CO2 and H2 gas. It can use the mixed gas of pure CO2 and H2 as a carbon source and energy source for growth and reproduction.

[0050] Example 3

[0051] This embodiment provides an experiment on the utilization effect of Aspergillus S8 on a mixture of CH4 and H2 gases. The steps are as follows:

[0052] Add 50 mL of inorganic salt culture medium to a 250 mL sealed bottle. Replace the headspace gas in the bottle with nitrogen. Add pure CH4 and H2 gas into the bottle, dividing it into three groups according to volume ratio: MH1 (CH4: H2 = 1:2), MH2 (CH4: H2 = 1:1), and MH3 (CH4: H2 = 2:1). Re-add the same volume ratio of mixed gas to the sealed bottle every 7 days, with each cycle lasting 7 days and repeated 4 times. Inoculate each bottle with 2% of the isolated and purified Aspergillus S8 suspension. Add ambient air to the control group bottle as a control.

[0053] After static incubation at 28℃ for 7 days, the average utilization rate of Aspergillus S8 for the CH4 and H2 mixed gas over 4 cycles was determined using a gas chromatography-mass spectrometry (GC2014C, purchased from Shimadzu Corporation). The results are shown in Table 3. The test conditions were the same as in Example 1. The average volume utilization rate over 4 cycles was determined by the water displacement gas collection method, and the results are shown in Table 3. The dry weight of the mycelium in the 4th cycle was determined in the same manner as in Example 2, and the results are shown in Table 3.

[0054] Table 3. Utilization efficiency of Aspergillus S8 on CH4 and H2 mixed gas over 4 cycles

[0055]

[0056] Table 3 shows that after adding Aspergillus S8 to the sealed bottle, the utilization rate of ambient air gas was only 3.124%, the volume utilization rate was 3.560%, and the mycelial dry weight was 30.561 mg. However, when Aspergillus S8 was added to a pure CH4 and H2 mixture, the utilization capacity of group MH3 was significantly higher, followed by group MH2 and then group MH1. This strain can efficiently utilize the pure CH4 and H2 mixture as a carbon and energy source for its growth and reproduction. Group MH3 achieved a CH4 and H2 mixture utilization rate of 94.620%, a volume utilization rate of 54.958%, and a mycelial dry weight of 89.350 mg. In the pure CH4 and H2 mixture, the gas utilization rate of Aspergillus S8 was 30.288 times that of the control group, the volume utilization rate was 15.437 times that of the control group, and the mycelial dry weight was 2.923 times that of the control group. This indicates that the Aspergillus S8 provided by the present invention has a good utilization effect on pure CH4 and H2 gases, with a strong utilization rate of pure CH4 and H2 gases, and can use pure CH4 and H2 gases as carbon and energy sources for growth and reproduction.

[0057] Example 4

[0058] This embodiment provides an experiment on the utilization effect of Aspergillus S8 on a mixture of CO2 and CH4 gases. The steps are as follows:

[0059] Add 50 mL of inorganic salt culture medium to a 250 mL sealed bottle. Replace the headspace gas in the bottle with nitrogen. Add pure CO2 and CH4 gas into the bottle, dividing it into three groups according to volume ratio: CM1 (CO2:CH4 = 1:2), CM2 (CO2:CH4 = 1:1), and CM3 (CO2:CH4 = 2:1). Re-add the same volume ratio of mixed gas to the sealed bottle every 7 days, with each cycle lasting 7 days and repeated 4 times. Inoculate each bottle with 2% of a purified suspension of Aspergillus S8. Add ambient air to the control group bottle as a control.

[0060] After static incubation at 28℃ for 7 days, the average utilization rate of Aspergillus S8 for a mixture of CO2 and CH4 over four cycles was determined using a gas chromatography-mass spectrometry (GC2014C, purchased from Shimadzu Corporation). The results are shown in Table 4. The test conditions were the same as in Example 1. The average volume utilization rate over four cycles was determined by the water displacement gas collection method, and the results are shown in Table 4. The dry weight of the mycelium in the fourth cycle was determined in the same manner as in Example 1, and the results are shown in Table 4.

[0061] Table 4. Utilization efficiency of Aspergillus S8 on CO2 and CH4 mixed gas over 4 cycles.

[0062]

[0063] Table 4 shows that after adding Aspergillus S8 to the sealed bottle, the utilization rate of ambient air gas was only 3.124%, the volume utilization rate was 3.560%, and the mycelial dry weight was 30.561 mg. However, when Aspergillus S8 was added to a mixture of pure CO2 and CH4 gas, the utilization capacity of group CM3 was significantly higher, followed by groups CM2 and CM1. This strain can efficiently utilize the mixture of pure CO2 and CH4 gas as a carbon source and energy source for its growth and reproduction. The utilization rate of CO2 and CH4 gas in group CM3 was 97.014%, the volume utilization rate was 73.632%, and the mycelial dry weight was 91.771 mg. The gas utilization rate of Aspergillus S8 in the mixture of pure CO2 and CH4 gas was 31.054 times that of the control group, the volume utilization rate was 20.683 times that of the control group, and the mycelial dry weight was 3.002 times that of the control group. This indicates that the Aspergillus S8 provided by the present invention has a good utilization effect on the mixed gas of pure CO2 and CH4, and has a strong utilization rate of the mixed gas of pure CO2 and CH4. It can use the mixed gas of pure CO2 and CH4 as a carbon source and energy source for growth and reproduction.

[0064] Example 5

[0065] This embodiment provides an experiment on the utilization effect of Aspergillus S8 on a mixture of CO2, CH4, and H2 gases. The steps are as follows:

[0066] Add 50 mL of inorganic salt culture medium to a 250 mL sealed bottle. Replace the headspace gas in the bottle with nitrogen. Add pure CO2, CH4, and H2 gases in a 1:1:1 volume ratio. Refill the sealed bottle with the same gas mixture every 7 days, repeating this cycle 4 times. Inoculate each bottle with 2% of a purified Aspergillus S8 suspension. Add ambient air to the control group bottle as a control.

[0067] After static incubation at 28℃ for 7 days, the average utilization rate of Aspergillus S8 for a mixture of CO2, CH4, and H2 gases over four cycles was determined using a gas chromatography-mass spectrometry (GC2014C, purchased from Shimadzu Corporation). The results are shown in Table 5. The test conditions were the same as in Example 1. The average volume utilization rate over four cycles was determined by the water displacement gas collection method, and the results are shown in Table 5. The dry weight of the mycelium in the fourth cycle was determined in the same manner as in Example 1, and the results are shown in Table 5.

[0068] Table 5. Utilization efficiency of Aspergillus S8 on a mixture of CO2, CH4, and H2 gases over four cycles.

[0069]

[0070] Table 5 shows that after adding Aspergillus S8 to the sealed bottle, the utilization rate of ambient air gas was only 3.124%, the volume utilization rate was 3.560%, and the mycelial dry weight was 30.561 mg. However, when Aspergillus S8 was added to a mixture of pure CO2, CH4, and H2 gas, the utilization capacity of the treatment group was significantly higher. This strain can efficiently utilize the mixture of pure CO2, CH4, and H2 gas as a carbon source and energy source for its growth and reproduction. The utilization rate of the treatment group for the mixture of pure CO2, CH4, and H2 gas was 94.722%, the volume utilization rate was 59.196%, and the mycelial dry weight was 86.768 mg. The gas utilization rate of Aspergillus S8 in the mixture of pure CO2, CH4, and H2 gas was 30.321 times that of the control group, the volume utilization rate was 16.628 times that of the control group, and the mycelial dry weight was 2.839 times that of the control group. This indicates that the Aspergillus S8 provided by the present invention has a good utilization effect on the mixed gas of pure CO2, CH4 and H2, and has a strong utilization rate of the mixed gas of pure CO2, CH4 and H2. It can use the mixed gas of pure CO2, CH4 and H2 as a carbon source and energy source for growth and reproduction.

[0071] From the above embodiments, it can be concluded that the Aspergillus S8 of the present invention can efficiently metabolize flammable and explosive gases as well as mixed gases containing flammable and explosive gases.

[0072] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. Aspergillus ( Aspergillus cejpii The application of S8 in gas metabolism is characterized by, The gas includes hydrogen or a mixture of gases containing hydrogen; the Aspergillus S8 has the accession number CGMCC NO.40828.

2. The application of an agent containing Aspergillus S8 in gas metabolism, characterized in that, The gas includes hydrogen or a mixture of gases containing hydrogen; the Aspergillus S8 has the accession number CGMCC NO.40828.

3. The application according to claim 2, characterized in that, The microbial agent includes a suspension of Aspergillus S8.

4. The application according to any one of claims 1 to 3, characterized in that, The mixed gas includes one or more of the following three: 1) Hydrogen and carbon dioxide; 2) Hydrogen and methane; 3) Hydrogen, methane, and carbon dioxide.

5. The application according to claim 4, characterized in that, 1) In the mixed gas, the volume ratio of hydrogen to carbon dioxide is (1~2):(1~2).

6. The application according to claim 4, characterized in that, 2) In the mixed gas, the volume ratio of hydrogen to methane is (1~2):(1~2).

7. The application according to claim 4, characterized in that, 3) In the mixed gas, the volume ratio of hydrogen, methane and carbon dioxide is 1:1:

1.

8. A method for gas metabolism, characterized in that, The gas was treated with Aspergillus S8 or an agent containing Aspergillus S8; the gas was hydrogen or a mixture containing hydrogen; the preservation number of Aspergillus S8 was CGMCC NO.40828.

9. The method according to claim 8, characterized in that, The inoculant is a bacterial suspension of Aspergillus S8.

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