Micro-fluidic chip card box for double-sample PCR (Polymerase Chain Reaction) detection

Abstract

The invention discloses a micro-fluidic chip card box for double-sample PCR (Polymerase Chain Reaction) detection. The micro-fluidic chip card box comprises a cover buckle, a cover body and a chip, the cover body comprises a cover body side wall, a first one-way valve, a second one-way valve and a rubber ring, the first one-way valve, the second one-way valve and the rubber ring are sequentially arranged on the inner wall of the cover body side wall, the cover body side wall, the first one-way valve and the second one-way valve form a closed cavity, and the cover body side wall, the second one-way valve and the rubber ring form an embedded cavity; a first partition plate is bonded between the first one-way valve and the second one-way valve, and a second partition plate is bonded between the second one-way valve and the rubber ring. The first partition plate and the second partition plate equally divide the closed cavity and the embedded cavity into a first section and a second section correspondingly. A PCR amplification agent, a first liquid bag and a second liquid bag are arranged in the embedded cavity; the cover buckle comprises a buckling piece, a gasket and a sealing piece; the sealing piece sequentially penetrates through the first one-way valve and the second one-way valve to stretch into the embedded cavity. The problems that fixation is unstable, operation is tedious, parallel group reference amplification and detection cannot be achieved, and aerosol pollution is caused are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and more particularly to a microfluidic chip cartridge for dual-sample PCR detection. Background Art

[0002] The microfluidic chip PCR cartridge is a detection tool that combines microfluidics and PCR technologies. By integrating nucleic acid extraction, amplification, and detection into a miniaturized chip, it enables rapid, efficient, and automated nucleic acid detection. The microfluidic chip cartridge integrates sample pretreatment, nucleic acid extraction, target gene amplification, real-time fluorescence detection, and result interpretation into a closed chip, reducing sample and reagent consumption while also minimizing manual operation and contamination risks. Its workflow typically includes reagent loading, nucleic acid extraction, nucleic acid elution, PCR amplification detection, and result analysis. It has broad and practical applications in clinical molecular testing, genetic screening, basic scientific research, animal and plant pathogen detection, and food hygiene and quarantine. With its highly integrated and automated features, the microfluidic chip PCR cartridge provides a fast, efficient, and portable solution for nucleic acid detection, making it particularly suitable for rapid on-site testing and point-of-care diagnosis.

[0003] Chinese patent application document 1 (application number: 2024108464385, application date: 2024.06.27) discloses a microfluidic chip cartridge for PCR detection, comprising: an anti-overflow soft plug 3', a cavity 5' pre-embedded with PCR amplification reagents 4', a microfluidic chip 6' and a cover 7', wherein: at least one reagent chamber 16' is provided in the cavity 5', at least one one-way soft valve is provided on the anti-overflow soft plug 3', and the anti-overflow soft plug 3' is used to seal the cavity 5' and the extracted sample liquid is injected into the cavity 5' through at least one one-way soft valve and sealed; a flow channel mechanism 14' is provided at the bottom of the cavity 5', and the flow channel mechanism 14' is connected to at least one reagent chamber 16' in the cavity 5'. The sample liquid mixed with the PCR amplification reagent 4' enters the reaction chamber of the microfluidic chip 6' through the flow channel mechanism 14'; the cover buckle 7' is engaged with the flow channel mechanism 14', and is used to clamp the microfluidic chip 6' between the cover buckle 7' and the flow channel mechanism 14'. The above device is prone to unstable fixation of the microfluidic chip 6', and the connection between the microfluidic chip 6' and the reagent chamber 16' requires correction and alignment, and the operation of additionally installing the microfluidic chip 6' is cumbersome; in addition, the above device can only perform multiple specific primer amplification of a single sample and cannot meet the requirements of parallel group reference detection of the same sample; further, the sample liquid is prone to aerosol contamination between the connection between the microfluidic chip 6' and the flow channel mechanism 14'.

[0004] Chinese patent application document 2 (application number: 2019108032982, application date: 2019.08.28) discloses a clamping device and detection method that integrates PCR amplification and capillary electrophoresis analysis. The method is performed using a clamping device, and the method includes: (1) filling the capillary electrophoresis system with glue; (2) under the action of a liquid suction and delivery device, the sample to be tested enters the PCR amplification area from the sample to be tested entrance for PCR amplification; (3) under the action of a liquid divider, the PCR amplified solution is mixed with the electrophoresis analysis reagent in proportion and then enters the temperature control area for heating denaturation; (4) the mixed solution after denaturation enters the capillary electrophoresis system for capillary electrophoresis analysis. The above scheme has a limited number of sample measurements, and can only perform single sample detection; further, the operation is cumbersome, and glue filling and reagent preparation in proportion are required in real time. The use of the clamping device is labor-intensive, and multiple sample detection is required.

[0005] Chinese patent application document 3 (application number: 202110210909X, application date: 2021.02.25) discloses a microfluidic detection chip and a microfluidic detection method, wherein the microfluidic detection chip includes at least one detection unit, and the detection unit includes a sample loading chamber, an immune binding pool, an immune detection pool, an immune cleaning liquid pool, an immune buffer pool, a nucleic acid detection pool, and a waste liquid pool. The above scheme integrates the sample pretreatment step, and there is also the problem that only a single sample can be detected. At the same time, this method cannot be well integrated into a convenient card box. Each pool needs to be loaded with samples immediately, which is cumbersome to use.

[0006] Therefore, it is a technical problem that needs to be solved urgently in this field to provide a microfluidic chip cartridge that is firmly fixed, easy to use, and can perform single sample parallel group reference amplification and detection, and prevent aerosol contamination of double sample PCR detection. Summary of the Invention

[0007] In view of this, the present invention provides a microfluidic chip cartridge for dual-sample PCR detection to solve the problems of unstable fixation, cumbersome operation, and inability to perform parallel group reference amplification and detection of two samples to prevent aerosol contamination.

[0008] The present application provides a microfluidic chip cartridge for dual-sample PCR detection, comprising a cover buckle, a cover body, and a circular chip, wherein the cover buckle covers the cover body, and the cover body, the cover buckle, and the chip are coaxially arranged;

[0009] The cover body includes a cover body side wall of a hollow cylindrical body, a circular first one-way valve, a circular second one-way valve and a circular rubber ring. The first one-way valve, the second one-way valve and the rubber ring are sequentially arranged on the inner wall of the cover body side wall along the direction in which the cover buckle points to the cover body. A closed cavity is formed between part of the cover body side wall, the first one-way valve and the second one-way valve, and a pre-buried cavity is formed between part of the cover body side wall, the second one-way valve and the rubber ring. Along the direction in which the cover buckle points to the cover body, the orthographic projection of the first one-way valve, the orthographic projection of the second one-way valve and the orthographic projection of the rubber ring are The first and second one-way valves are completely overlapped; a rectangular first partition is bonded between the central axis of the first one-way valve and the central axis of the second one-way valve, and a rectangular second partition is bonded between the central axis of the second one-way valve and the central axis of the rubber ring; along the direction from the cover buckle to the cover body, the orthographic projection of the first partition and the orthographic projection of the second partition completely overlap; the first partition and the second partition respectively divide the sealed cavity and the embedded cavity into a first section and a second section, the first section of the sealed cavity corresponds to the first section of the embedded cavity, and the second section of the sealed cavity corresponds to the second section of the embedded cavity;

[0010] Along the direction from the cover buckle to the cover body, the rubber ring is provided with an arc-shaped lower sampling hole running through its thickness. The number of the lower sampling holes is two, and the two lower sampling holes are respectively connected to the corresponding embedded cavity of the first section and the corresponding embedded cavity of the second section. The two lower sampling holes are mirror-symmetrical with the second partition as the symmetry plane;

[0011] A first liquid capsule is bonded to a side of the second one-way valve corresponding to the first section away from the first one-way valve, and a second liquid capsule is bonded to a side of the second one-way valve corresponding to the second section away from the first one-way valve; both the first and second liquid capsules are filled with a buffer solution; and both the pre-buried cavity corresponding to the first section and the pre-buried cavity corresponding to the second section are provided with a PCR amplification agent.

[0012] The cover buckle is clamped on the side of the first one-way valve away from the sealed cavity, and comprises a hollow cylindrical buckle, a circular gasket and a conical sealing member bonded in sequence along the direction of the cover buckle toward the cover body, wherein the buckle is bonded to the gasket on the side close to the gasket;

[0013] The diameter of the fastener is equal to the outer diameter of the side wall of the cover body, and the fastener is fastened to the top of the side wall of the cover body; the diameter of the gasket is equal to the inner diameter of the side wall of the cover body, and it contacts and fastens with the first one-way valve inside the side wall of the cover body, and a gasket through-hole is opened on the gasket and penetrates the gasket in the direction from the cover buckle to the cover body, and the number of gasket through-holes is two, and the two gasket through-holes correspond to the first liquid capsule and the second liquid capsule respectively; the number of sealing members is two, and the tips of the two sealing members are both facing the side of the first one-way valve, and the two sealing members are respectively inserted into the two gasket through-holes; when the sealing members are in the fastened state, the tips of the two sealing members respectively penetrate the first one-way valve and the second one-way valve in sequence along the direction from the cover buckle to the cover body and extend into the embedded cavity;

[0014] The chip is divided into a first semicircular piece and a second semicircular piece with the same structure with the plane where the second partition is located as the symmetry axis. The first piece and the second piece both include a circular base and a cover piece bonded to the base. The cover piece matches the base. The base is located on the side of the cover piece close to the rubber ring, and the side of the base close to the cover piece is connected to the rubber ring. The bases of the first piece and the second piece are respectively provided with flow channel injection holes corresponding to the two lower injection holes.

[0015] Compared with the prior art, the microfluidic chip cartridge for dual-sample PCR detection provided by the present invention achieves at least the following beneficial effects:

[0016] First, the present invention provides a microfluidic chip cartridge for dual-sample PCR detection, which can perform instant parallel group reference amplification and detection of a single sample. First, the present invention provides a cover sidewall that can be divided into a first section and a second section for sampling, allowing two injections to be performed in the first and second sections of the cover sidewall, respectively. Secondly, the present invention also provides a first and second chip for amplification and detection corresponding to the first and second sections of the cover sidewall. After the sample solution is mixed with the PCR amplifier and buffer in the cover sidewall, it can be synchronously introduced into the first and second chips of the chip, allowing the sample to be synchronously amplified and detected instantly, enhancing the scientific nature of sample amplification and detection and reducing detection errors.

[0017] Secondly, a microfluidic chip cartridge for dual-sample PCR detection provided by the present invention can prevent detection contamination and inaccurate detection caused by aerosols; firstly, the present invention provides a first one-way valve and a second one-way valve to prevent external gas from contaminating the side wall detection of the cover body; secondly, the present invention sets the first one-way valve and the second one-way valve as double seals to prevent contamination of the pipette gun during the sampling process; further, the present invention also provides a seal to elastically seal the first one-way valve and the second one-way valve, further enhancing the possibility of preventing aerosol contamination; and, the present invention also provides a fixed sealed connection relationship between the chip and the side wall of the cover body, and plugs a rubber ring therein to reduce the problem of mutual contamination of samples in the process of entering the chip.

[0018] Third, the present invention provides a dual-sample PCR detection microfluidic chip cartridge that is simple to use and securely secures the chip. The present invention pre-embeds the PCR amplifier and buffer separately, and further pre-embeds specific primers and catalytic enzymes in the chip. By pre-embedding all necessary elements, the present invention requires only simple sample pretreatment before injection. For example, if the sample is a microscopically exposed template, it can be directly injected for amplification testing, further simplifying the formulation of the reaction solution. Furthermore, the present invention integrates the chip with the sidewall of the cover, enhancing the chip's stability during centrifugation and simplifying the chip installation process.

[0019] Of course, any product implementing the present invention does not necessarily need to achieve all of the technical effects described above at the same time.

[0020] Further features and advantages of the present invention will become apparent from the following detailed description of exemplary embodiments of the present invention with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and, together with the description, serve to explain the principles of the invention.

[0022] Figure 1 This is a schematic diagram of the structure of a microfluidic chip cartridge for PCR detection provided in Chinese patent application document 1;

[0023] Figure 2 Schematic diagram of the structure of the microfluidic chip cartridge for dual-sample PCR detection provided by the present invention;

[0024] Figure 3 Schematic diagram of the assembly of the microfluidic chip cartridge for dual-sample PCR detection provided by the present invention;

[0025] Figure 4 is an AA' cross-sectional view of the cover provided by the present invention;

[0026] Figure 5 A BB' cross-sectional view of the cover body and the cover buckle provided by the present invention;

[0027] Figure 6 1 is a schematic diagram of the top view of the chip provided by the present invention;

[0028] Figure 7 yes Figure 5 Enlarged view of point C in the middle;

[0029] Figure 8 yes Figure 2 Enlarged view of point D in the middle. DETAILED DESCRIPTION

[0030] Various exemplary embodiments of the present invention will now be described in detail with reference to the accompanying drawings. It should be noted that unless otherwise specifically stated, the relative arrangement of components and steps, numerical expressions and numerical values ​​set forth in these embodiments do not limit the scope of the present invention.

[0031] The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the invention, its application, or uses.

[0032] Technologies, methods, and equipment known to ordinary technicians in the relevant art may not be discussed in detail, but where appropriate, the technologies, methods, and equipment should be considered part of the specification.

[0033] In all examples shown and discussed herein, any specific values ​​should be interpreted as merely exemplary and not limiting. Therefore, other examples of the exemplary embodiments may have different values.

[0034] It should be noted that like reference numerals and letters refer to like items in the following figures, and therefore, once an item is defined in one figure, it need not be further discussed in subsequent figures.

[0035] Example 1

[0036] Reference Figure 2-Figure 6 As shown, Figure 2 Schematic diagram of the structure of the microfluidic chip cartridge for dual-sample PCR detection provided by the present invention; Figure 3 Schematic diagram of the assembly of the microfluidic chip cartridge for dual-sample PCR detection provided by the present invention; Figure 4 is an AA' cross-sectional view of the cover provided by the present invention; Figure 5 A BB' cross-sectional view of the cover body and the cover buckle provided by the present invention; Figure 6 : This is a schematic diagram of the top view of the chip provided by the present invention; this embodiment provides a microfluidic chip cartridge for dual-sample PCR detection, comprising a cover buckle 1, a cover body 2, and a circular chip 3. The cover buckle 1 covers the cover body 2, and the cover body 2, cover buckle 1, and chip 3 are coaxially arranged.

[0037] The cover body 2 includes a cover body side wall 20 of a hollow cylindrical body, a circular first one-way valve 23, a circular second one-way valve 24 and a circular rubber ring 26. The first one-way valve 23, the second one-way valve 24 and the rubber ring 26 are sequentially arranged on the inner wall of the cover body side wall 20 along the direction Z from the cover buckle 1 to the cover body 2. A closed cavity 21 is formed between part of the cover body side wall 20, the first one-way valve 23 and the second one-way valve 24, and a pre-buried cavity 22 is formed between part of the cover body side wall 20, the second one-way valve 24 and the rubber ring 26. Along the direction Z from the cover buckle 1 to the cover body 2, the orthographic projections of the first one-way valve 23, the orthographic projections of the second one-way valve 24 and the rubber ring 26 are completely intersected. A rectangular first partition 25 is bonded between the central axis of the first one-way valve 23 and the central axis of the second one-way valve 24, and a rectangular second partition 27 is bonded between the central axis of the second one-way valve 24 and the central axis of the rubber ring 26; along the direction Z from the cover buckle 1 to the cover body 2, the orthographic projection of the first partition 25 and the orthographic projection of the second partition 27 completely overlap; the first partition 25 and the second partition 27 respectively divide the closed cavity 21 and the embedded cavity 22 into a first section 021 and a second section 022, the first section 021 in the closed cavity 21 corresponds to the first section 021 in the embedded cavity 22, and the second section 022 in the closed cavity 21 corresponds to the second section 022 in the embedded cavity 22;

[0038] Along the direction Z from the cover buckle 1 to the cover body 2, the rubber ring 26 is provided with an arc-shaped lower sampling hole 28 running through its thickness. The number of the lower sampling holes 28 is two, and the two lower sampling holes 28 are respectively connected to the embedded cavity 22 corresponding to the first section 021 and the embedded cavity 22 corresponding to the second section 022. The two lower sampling holes 28 are mirror-symmetrical with the second partition 27 as the symmetry plane;

[0039] A first liquid capsule 221 is bonded to the side of the second one-way valve 24 corresponding to the first section 021 away from the first one-way valve 23, and a second liquid capsule 222 is bonded to the side of the second one-way valve 24 corresponding to the second section 022 away from the first one-way valve 23. Both the first liquid capsule 221 and the second liquid capsule 222 are filled with buffer. Both the pre-buried cavity 22 corresponding to the first section 021 and the pre-buried cavity 22 corresponding to the second section 022 are provided with a PCR amplification agent 223.

[0040] The cover buckle 1 is clamped to the side of the first one-way valve 23 away from the sealed chamber 21. It includes a hollow cylindrical fastener 11, a circular gasket 12, and a conical sealing member 13 bonded in sequence along the direction Z of the cover buckle 1 pointing to the cover body 2. The fastener 11 is bonded to the gasket 12 on the side closest to the gasket 12.

[0041] The diameter of the fastener 11 is equal to the outer diameter of the cover side wall 20, and the fastener 11 is fastened to the top of the cover side wall 20; the diameter of the gasket 12 is equal to the inner diameter of the cover side wall 20, and it contacts and fastens with the first one-way valve 23 in the cover side wall 20, and is provided with a gasket through hole that penetrates the gasket 12 along the direction Z pointing from the cover buckle 1 to the cover body 2, and the number of the gasket through holes is two, and the two gasket through holes correspond to the first liquid capsule 221 and the second liquid capsule 222 respectively; the number of the sealing members 13 is two, and the tips of the two sealing members 13 are both facing the side of the first one-way valve 23, and the two sealing members 13 are respectively inserted into the two gasket through holes; when the sealing members 13 are in the fastened state, along the direction Z pointing from the cover buckle 1 to the cover body 2, the tips of the two sealing members 13 respectively penetrate the first one-way valve 23 and the second one-way valve 24 in sequence and extend into the embedded cavity 22;

[0042] The chip 3 is divided into a first semicircular piece 31 and a second semicircular piece 32 with the same structure with the plane where the second partition 27 is located as the symmetrical plane. The first piece 31 and the second piece 32 both include a circular base and a cover piece bonded to the base. The cover piece matches the base. The base is located on the side of the cover piece away from the rubber ring 26, and the side of the cover piece away from the base is connected to the rubber ring 26. The cover pieces of the first piece 31 and the second piece 32 are respectively provided with flow channel injection holes corresponding to the two lower injection holes 28.

[0043] Specifically, continue to refer to Figure 2-Figure 4 As shown, along the direction Z pointing from the cover buckle 1 to the cover body 2, this embodiment provides a microfluidic chip card box for dual-sample PCR detection, including a cover buckle 1, a cover body 2 and a chip 3 coaxially connected and penetrated in sequence, the chip 3 is fixedly and tightly bonded to the cover body 2, and the cover buckle 1 is movably engaged with the cover body 2.

[0044] The cover body 2 is a hollow cylinder of uniform thickness, comprising a tubular cover sidewall 20 with openings at both ends. The cover sidewall 20 comprises a sealed cavity 21 and a pre-buried cavity 22, which extend one-way through the interior of the cover sidewall 20 in the direction Z from the cover buckle 1 to the cover body 2. The sealed cavity 21 and the pre-buried cavity 22 are formed by a first one-way valve 23, a second one-way valve 24, and a rubber ring 26, which are arranged sequentially within the cover sidewall 20 in the direction Z from the cover buckle 1 to the cover body 2. The first one-way valve 23, the second one-way valve 24, and a portion of the cover sidewall 20 form the cylindrical sealed cavity 21; the second one-way valve 24, the rubber ring 26, and another portion of the cover sidewall 20 form the cylindrical pre-buried cavity 22.

[0045] The central axes of the first and second one-way valves 23 and 24 coincide with each other. A first partition 25 is bonded and fixed between the first and second one-way valves 23 and 24, passing through the central axis of the first or second one-way valve 23 or 24. The central axes of the rubber ring 26 and the second one-way valve 24 also coincide with each other. A second partition 27 is bonded and fixed between the rubber ring 26 and the second one-way valve 24, passing through the central axis of the rubber ring 26 or the second one-way valve 24. The central axes of the first and second partitions 25 and 27 along the direction Z from the cover buckle 1 to the cover body 2 coincide with the central axes of the cover sidewall 20, the rubber ring 26, and the first or second one-way valve 23 or 24. The first and second partitions 25 and 27 also coincide with the center of the chip 3. The first and second partitions 25 and 27 are both rectangular plates of the same width. The width of the first and second partitions 25 and 27 perpendicular to the length of the cover sidewall 20 is equal to the diameter of the first or second one-way valve 23 or 24. Along the direction Z pointing from the cover buckle 1 to the cover body 2, the orthographic projection of the first partition 25 and the orthographic projection of the second partition 27 overlap. The first partition 25 and the second partition 27 divide the cover sidewall 20 into a first section 021 and a second section 022. The first section 021 and the second section 022 are each a semi-cylinder, symmetrical in structure and size, and are used to simultaneously test two samples.

[0046] The first one-way valve 23 is bonded to the cover sidewall 20 on the side closest to the cover buckle 1. The first one-way valve 23 is a circular rubber sheet. The first one-way valve 23 forms the upper bottom surface of the cover sidewall 20, and the diameter of the first one-way valve 23 is equal to the inner diameter of the cover sidewall 20. A second one-way valve 24 is bonded to the side of the sealed chamber 21 away from the first one-way valve 23. The second one-way valve 24 is a circular rubber sheet with the same structure and size as the first one-way valve 23. Along the direction Z from the cover buckle 1 to the cover 2, the orthographic projection of the second one-way valve 24 overlaps with the orthographic projection of the first one-way valve 23. The excellent elasticity of rubber allows the first and second one-way valves 23 and 24 to maintain an elastically locked state in their natural state.

[0047] The side of the second one-way valve 24 away from the sealed cavity 21 is the embedded cavity 22, which is a cylindrical cavity. A rubber ring 26 is provided on the side of the embedded cavity 22 away from the second one-way valve 24, which is overlapped and glued to the lower bottom surface of the cover side wall 20. The rubber ring 26 is circular in shape. The rubber ring 26 is made of rubber with different elasticity from the first one-way valve 23 and the second one-way valve 24. The diameter of the rubber ring 26 is equal to the inner diameter length of the cover side wall 20. Along the direction Z from the cover buckle 1 to the cover body 2, the rubber ring 26 protrudes at least 0.1 mm from the lower bottom surface of the cover side wall 20 away from the upper bottom surface of the cover side wall 20. The protrusion of at least 0.1 mm allows the rubber ring 26 to be elastically squeezed after the cover side wall 20 is bonded to the chip 3, further enhancing the bonding and sealing between the lower bottom surface of the cover side wall 20 and the chip 3. The rubber ring 26 is provided with two lower sampling holes 28 corresponding to the pre-buried cavity 22 in the first section 021 and the pre-buried cavity 22 in the second section 022 along the direction Z from the cover buckle 1 to the cover body 2. The lower sampling hole 28 is a rectangular hole with an arc bend, and the arc bending direction is the central axis direction of the cover body side wall 20. The two lower sampling holes 28 are mirror-symmetrical with the second partition 27 as the symmetry plane. After the pipette tip passes through the first one-way valve 23 and the second one-way valve 24, the sample is input into the pre-buried cavity 22 and enters the different flow channel sampling holes of the chip 3 through the two lower sampling holes 28.

[0048] The pre-buried cavity 22 corresponding to the first section 021 and the pre-buried cavity 22 corresponding to the second section 022 are both filled with a PCR amplification agent 223 (Polymerase Chain Reaction, polymerase chain reaction); the PCR amplification agent 223 is a lyophilized agent, and the PCR amplification agent 223 includes dNTP and Mg 2+ The freeze-dried PCR amplifier 223 is a conventional technical means in this field, and the technical personnel can easily know it through the description. This embodiment does not elaborate on the freeze-dried PCR amplifier 223.

[0049] In the first section 021, a first liquid capsule 221 is bonded to the side of the second one-way valve 24 facing away from the first one-way valve 23. In the second section 022, a second liquid capsule 222 is bonded to the side of the second one-way valve 24 facing away from the first one-way valve 23. The first and second liquid capsules 221 and 222 are ellipsoidal in shape and are specifically made of polyester film. The first and second liquid capsules 221 and 222 are identical in size. Both the first and second liquid capsules 221 and 222 contain a buffer solution, which may be Tris-HCl buffer. When the pipette tip passes through the first and second one-way valves 23 and 24 and enters the pre-buried cavity 22, the pipette tip automatically punctures the first or second liquid capsule 221 or 222, allowing the buffer solution in the first or second liquid capsule 221 or 222 to flow into the pre-buried cavity 22 and mix with the sample and PCR amplification agent 223 within the pre-buried cavity 22.

[0050] Continue to refer to Figure 2-Figure 5 As shown, the cover buckle 1 is a cylindrical structure of uniform thickness. Its length extends in the direction Z from the cover buckle 1 toward the cover body 2. The cover buckle 1 is secured to the upper bottom surface of the cover body sidewall 20 and contacts the side of the first one-way valve 23 facing away from the sealed chamber 21. Along the direction Z from the cover buckle 1 toward the cover body 2, the cover buckle 1 includes a fastening member 11, a gasket 12, and a sealing member 13.

[0051] The fastener 11 is cylindrical in shape, with a diameter equal to the outer diameter of the cover sidewall 20. The side of the fastener 11 proximal to the first one-way valve 23 is bonded to the gasket 12 and the seal 13. The fastener 11 is designed to be held by the user, allowing the cover buckle 1 to snap onto the end of the cover sidewall 20 facing away from the chip 3, thereby closing the end of the cover sidewall 20 facing away from the chip 3. The fastener 11 is made of plastic, which is easy to connect, easy to manufacture, and lightweight.

[0052] The gasket 12 is a circular piece with the same diameter as the inner diameter of the cover sidewall 20. One wide surface of the gasket 12 is bonded to the side of the fastener 11 closest to the cover sidewall 20, and the central axis of the gasket 12 coincides with the central axis of the fastener 11. When the cover buckle 1 is fastened to the cover sidewall 20 via the fastener 11, the side of the gasket 12 facing away from the fastener 11 contacts the first one-way valve 23. The gasket 12 is provided with two gasket through-holes extending along the direction Z from the cover buckle 1 to the cover 2. These two gasket through-holes are mirror-symmetrical about the imaginary plane of the first partition 25. The gasket through-holes are circular holes with a radius smaller than the bottom radius of the sealing member 13. Along the direction Z from the cover buckle 1 to the cover 2, the orthographic projections of the two gasket through-holes coincide with the orthographic projections of the two sealing members 13. The gasket through hole is used for the sealing member 13 to pass through, so that the sealing member 13 can pass through the gasket 12 and directly be rigidly bonded to the side of the fastening member 11 close to the gasket 12.

[0053] The seal 13 is conical and is located on the side of the fastener 11 near the gasket 12. The two seals 13 are mirror-symmetrical about the imaginary plane of the first partition 25. The tips of the two seals 13 face the lower surface of the cover buckle 1. The tips of the two seals 13 respectively penetrate the gasket holes of the first section 021 and the second section 022, the first one-way valve 23 and the second one-way valve 24, and extend into the embedded cavity 22 of the first section 021 and the second section 022. Both seals 13 are made of rigid silicone or plastic. When the seals 13 pass through the first and second one-way valves 23 and 24, the rigid seals 13 can expand the silicone rubber of the first and second one-way valves 23 and 24, achieving an elastic interference fit between the first and second one-way valves 23 and 24, thereby enhancing the sealing performance of the first and second one-way valves 23 and 24.

[0054] Continue to refer to Figure 2 、 Figure 3 and Figure 6 As shown, the chip 3 is in the shape of a circular sheet and includes a circular base away from the rubber ring 26, and a cover sheet that overlaps and is bonded to the base and to the rubber ring 26. The central axes of the base, cover sheet, rubber ring 26, and the side wall 20 of the cover body overlap with each other. The base is made of silicon, and the cover sheet is made of glass. The silicon base and the cover sheet made of glass are both conventional technical means in this field and will not be described in detail in this embodiment. The base and the cover sheet are divided into a semicircular first sheet 31 and a second sheet 32, which are mirror-symmetrically arranged along the imaginary plane where the first partition is located. The semicircular first sheet 31 and the semicircular second sheet 32 ​​are made of an integrated structure. The first sheet 31 and the second sheet 32 ​​have the same structure and size and are both pre-embedded with a specific primer and a freeze-dried preparation of the corresponding catalytic enzyme. The side of the cover sheet of the first sheet 31 and the second sheet 32 ​​near the rubber ring 26 is provided with a flow channel injection hole corresponding to the two lower injection holes 28. The flow channel injection hole is a circular hole that passes through the cover sheet along the direction Z from the cover buckle 1 to the cover body 2. Through centrifugal rotation, the sample, PCR amplification agent 223, and buffer mixed in the pre-buried cavity 22 of the first section 021 enter the flow channel injection hole in the first sheet 31 through the rubber ring 26 corresponding to a lower injection hole 28 in the pre-buried cavity 22 of the first section 021. Simultaneously, through centrifugal rotation, the sample, PCR amplification agent 223, and buffer mixed in the pre-buried cavity 22 of the second section 022 enter the flow channel injection hole in the second sheet 32 ​​through the rubber ring 26 corresponding to a lower injection hole 28 in the pre-buried cavity 22 of the second section 022. Simultaneous amplification and detection of the two samples is achieved in the first sheet 31 and the second sheet 32.

[0055] In a specific implementation process, this embodiment can be used for rapid nucleic acid detection of pathogens (such as viruses and bacteria); it can also be used for rapid detection of pathogens in imported and exported goods at customs.

[0056] Continue to refer to Figure 2-Figure 6 As shown, the specific operations are as follows:

[0057] First, the sample is simply pre-treated externally. This step is a routine operation in this field and will not be described in detail.

[0058] Secondly, perform the first sample addition: use a pipette to absorb the sample solution, so that the pipette tip passes through the first one-way valve 23 and the second one-way valve 24 in the first section 021 along the direction Z that the cover buckle 1 points to the cover body 2, and extends into the pre-buried cavity 22 in the first section 021; in the process of the pipette tip extending, the pipette tip part of the pipette punctures the first liquid capsule 221 in the pre-buried cavity 22 of the first section 021, so that the buffer solution in the first liquid capsule 221 is released into the pre-buried cavity 22 of the first section 021 to mix with the PCR amplifier 223 in the pre-buried cavity 22 of the first section 021; in the pre-buried cavity 22 in the first section 021, the released sample solution is injected, so that the sample solution is further mixed with the PCR amplifier 223 and the buffer solution in the pre-buried cavity 22 of the first section 021; during the injection process, the pre-buried cavity 22 in the first section 021 should be appropriately pressurized by the pipette; pull out the pipette, and then discard the pipette tip.

[0059] The second sample addition is further performed: a new can is set for the pipette, and another sample solution is sucked with the pipette. The pipette tip passes through the first one-way valve 23 and the second one-way valve 24 in the second section 022 along the direction Z of the cover buckle 1 pointing to the cover body 2, and extends into the pre-buried cavity 22 in the second section 022; during the process of the pipette tip extending into the pre-buried cavity 22 in the second section 022, the pipette tip punctures the second liquid capsule 222 in the pre-buried cavity 22 of the second section 022, so that the buffer solution in the second liquid capsule 222 is absorbed. The flushing liquid is released into the pre-buried cavity 22 of the second section 022 to mix with the PCR amplifier 223 in the pre-buried cavity 22 of the second section 022; in the pre-buried cavity 22 in the second section 022, the sample solution is injected and released, so that the sample solution is further mixed with the PCR amplifier 223 and the buffer solution in the pre-buried cavity 22 of the second section 022; during the injection process, the pre-buried cavity 22 in the second section 022 should be appropriately pressurized by a pipette; the pipette is pulled out and the pipette tip is discarded immediately.

[0060] Then, along the direction Z from the cover buckle 1 toward the cover body 2, the fastening member 11 is fastened toward the upper bottom surface of the cover side wall 20, causing the fastening member 11 to engage with the upper bottom surface of the cover side wall 20. That is, the two sealing members 13 sequentially pass through the first and second one-way valves 23, 24, further enhancing the airtightness of the first and second one-way valves 23, 24; the gasket 12 is elastically squeezed and contacts the side of the first one-way valve 23 away from the sealed cavity 21.

[0061] Finally, the microfluidic chip cartridge for dual-sample PCR detection provided in this embodiment is placed in a PCR amplification instrument for centrifugation, temperature control, and monitoring.

[0062] Compared with the prior art, the microfluidic chip cartridge for dual-sample PCR detection provided in this embodiment achieves at least the following beneficial effects:

[0063] First, the microfluidic chip cartridge for dual-sample PCR detection provided in this embodiment can perform instant parallel group reference amplification and detection of a single sample. First, this embodiment provides a cover sidewall 20 that can be divided into two areas for sample injection, a first section 021 and a second section 022. Two injections can be performed in the first section 021 and the second section 022 of the cover sidewall 20, respectively. Second, this embodiment also provides a first chip 31 and a second chip 32 for amplification and detection corresponding to the first section 021 and the second section 022 of the cover sidewall 20. After the sample solution is mixed with the PCR amplification agent 223 and the buffer in the cover sidewall 20, it can be synchronously introduced into the first chip 31 and the second chip 32, allowing for simultaneous instant amplification and detection of the sample, enhancing the scientific nature of the sample amplification and detection, and reducing detection errors.

[0064] Secondly, the microfluidic chip cartridge for dual-sample PCR detection provided in this embodiment can prevent detection contamination and inaccurate detection caused by aerosols; first, this embodiment provides a first one-way valve 23 and a second one-way valve 24 to prevent external gas from contaminating the detection of the cover side wall 20; secondly, this embodiment sets the first one-way valve 23 and the second one-way valve 24 as double seals to prevent contamination of the pipette during the injection process; further, this embodiment also provides a seal 13 to elastically seal the first one-way valve 23 and the second one-way valve 24, further enhancing the possibility of preventing aerosol contamination; and, this embodiment also provides a fixed sealed connection relationship between the chip 3 and the cover side wall 20, and plugs a rubber ring 26 therein to reduce the problem of mutual contamination of samples in the process of entering the chip 3.

[0065] Third, the dual-sample PCR detection microfluidic chip cartridge provided by this embodiment is simple to use and securely secures the chip 3. First, this embodiment pre-embeds the PCR amplifier 223 and buffer separately, and further pre-embeds specific primers and a catalytic enzyme within the chip 3. This pre-embedded structure allows for simple sample pretreatment prior to injection. For example, if the sample is already a microscopically exposed template, it can be directly injected for amplification and detection, further simplifying the formulation of the reaction solutions. Furthermore, the integrated connection between the chip 3 and the side wall 20 of the cover body enhances the stability of the chip 3 during centrifugation and simplifies the installation of the chip 3.

[0066] In an alternative embodiment, continue to refer to Figure 2 、 Figure 4 and Figure 5As shown, the first one-way valve 23 and the second one-way valve 24 are respectively provided with corresponding circular upper injection holes 231. There are two upper injection holes 231, one corresponding to the first section 021 and the other corresponding to the second section 022. The first injection hole and the second injection hole correspond to the first liquid capsule 221 and the second liquid capsule 222, respectively. The first injection hole is used to pass through the closed cavity 21 and the embedded cavity 22 corresponding to the first section 021, and the second injection hole is used to pass through the closed cavity 21 and the embedded cavity 22 of the second section 022. The natural state of the first injection hole and the second injection hole is an elastic closed state.

[0067] When the sealing member 13 is in the buckled state, along the direction Z pointing from the cover buckle 1 to the cover body 2, the tip of the first sealing member 13 sequentially passes through the first injection hole of the first one-way valve 23 and the first injection hole of the second one-way valve 24 and extends into the embedded cavity 22. At the same time, the tip of the second sealing member 13 sequentially passes through the second injection hole of the first one-way valve 23 and the second injection hole of the second one-way valve 24 and extends into the embedded cavity 22.

[0068] Specifically, along the direction Z from the cover buckle 1 toward the cover body 2, the cover body sidewall 20 is provided with an upper sampling hole 231 that sequentially passes through the first one-way valve 23 and the second one-way valve 24. Two upper sampling holes 231 are provided, both of which are circular. Both upper sampling holes 231 are mirror-symmetrical about the imaginary plane of the first partition 25. The two upper sampling holes 231 are respectively a first sampling hole corresponding to the first section 021 and a second sampling hole corresponding to the second section 022.

[0069] The first injection hole is a through hole for penetrating the closed cavity 21 and the embedded cavity 22 in the first section 021. The radius of the first injection hole is not greater than 1 / 4 of the radius of the first one-way valve 23. The first injection hole is in an elastic closed state in a natural state due to the rubber material of the first one-way valve 23 and the second one-way valve 24. When the sealing member 13 is in a buckled state, along the direction Z pointing from the cover buckle 1 to the cover body 2, the tip of the first sealing member 13 can resist the rubber elasticity of the first one-way valve 23 and the second one-way valve 24, and sequentially penetrate the first injection hole of the first one-way valve 23 and the first injection hole of the second one-way valve 24 into the embedded cavity 22. The second injection hole is a through hole for penetrating the closed cavity 21 and the embedded cavity 22 in the second section 022. The radius of the second injection hole is not greater than 1 / 4 of the radius of the first one-way valve 23. The second injection hole is in an elastic closed state in a natural state due to the influence of the rubber material of the radius of the first one-way valve 23; when the sealing member 13 is in a buckled state, along the direction Z pointing from the cover buckle 1 to the cover body 2, the tip of the second sealing member 13 can resist the rubber elasticity of the first one-way valve 23 and the second one-way valve 24, and sequentially penetrate the second injection hole of the first one-way valve 23 and the second injection hole of the second one-way valve 24 and extend into the embedded cavity 22.

[0070] Through the above scheme, the first sampling hole is used for the first sample addition, and the pipette tip is passed through the first one-way valve 23 and the second one-way valve 24 corresponding to the first section 021 along the direction Z of the cover buckle 1 pointing to the cover body 2; the second sampling hole is used for the first sample addition, and the pipette tip is passed through the first one-way valve 23 and the second one-way valve 24 corresponding to the second section 022 along the direction Z of the cover buckle 1 pointing to the cover body 2.

[0071] In an alternative embodiment, continue to refer to Figure 2 and Figure 7 As shown, Figure 7 yes Figure 5 An enlarged view of point C in the middle; a snap ring 14 is fixedly bonded to the outer side of the snap member 11 near one end of the gasket 12 and away from the seal 13; the snap ring 14 extends along its circumferential direction and is a hollow ring body, and its cross-sectional shape is hook-shaped;

[0072] The side wall 20 of the cover body corresponds to the outer wall of the first one-way valve 23 and is fixedly bonded with a fixing ring 29 extending along its circumferential direction. The fixing ring 29 is a hollow annular body with an inverted L-shaped cross-section. The fixing ring 29 matches the buckling ring 14 and is buckled to the buckling ring 14.

[0073] Specifically, a snap ring 14 is fixedly bonded to the snap fitting 11 near one end of the gasket 12 and away from the outer side surface of the sealing member 13 along the circumferential direction of the snap fitting 11. The snap ring 14 is a hollow ring body, and its inner diameter is equal to the outer diameter of the snap fitting 11. The snap ring 14 is hook-shaped in any cross-sectional shape along its length extension direction. The hook head of the snap ring 14 is overlapped and bonded with the lower bottom surface of the cover body side wall 20, that is, the side of the hook head of the snap ring 14 near the first one-way valve 23 is at the same horizontal height as the lower bottom surface of the cover body side wall 20. The length extension direction of the hook head and the hook tail of the snap ring 14 is horizontal. The hook tail of the snap ring 14 is integrally provided with a rectangular anti-slip block, and the length extension directions of the anti-slip block and the hook tail of the snap ring 14 are perpendicular to each other. The anti-slip block is used to fix the fixing ring 29 to prevent the fixing ring 29 from falling off.

[0074] A retaining ring 29 is fixedly bonded along the circumference of the lid sidewall 20, near one end of the first one-way valve 23. The retaining ring 29 is a hollow annular body that is coaxially coupled to the snap ring 14. Any cross-section along its length is an inverted L-shaped. The head of the inverted L-shaped retaining ring 29 is fixedly bonded to the top surface of the lid sidewall 20 at the same height, and the entire inverted L-shaped retaining ring 29 snaps into engagement with the hook of the snap ring 14. The right-angled edge of the inverted L-shaped retaining ring 29, near the snap ring 14, is flattened. This flattened retaining ring 29 facilitates smoother engagement when the snap ring 14 snaps in the direction Z from the lid buckle 1 to the lid body 2.

[0075] Through the above scheme, the fixed fastening of the fastener and the side wall 20 of the cover body can be achieved, so that the fastener only needs to be pushed in the direction Z pointing from the cover buckle 1 to the cover body 2 on one side of the first one-way valve 23 of the side wall 20 of the cover body to achieve fastening and fixing, which is convenient for users to perform quick sealing operations, helps to reduce the operational practices and steps of fixed sealing, and thus helps to improve the overall detection speed.

[0076] In an alternative embodiment, continue to refer to Figure 2 、 Figure 4 and Figure 5 As shown, along the direction Z pointing from the cover buckle 1 to the cover body 2, the length of the sealing member 13 is a, the maximum distance between the side of the fastener 11 close to the gasket 12 and the side of the first liquid sac 221 away from the second one-way valve 24, and the maximum distance between the side of the fastener 11 close to the gasket 12 and the side of the second liquid sac 222 away from the second one-way valve 24 are b, a>b.

[0077] Specifically, the length of the sealing member 13 along the direction Z from the cover buckle 1 to the cover body 2 is equal to the height of the conical body of the sealing member 13, that is, the length of the sealing member 13 is a. The maximum distance between the side of the fastening member 11 near the gasket 12 and the side of the first liquid capsule 221 away from the second one-way valve 24, and the maximum distance between the side of the fastening member 11 near the gasket 12 and the side of the second liquid capsule 222 away from the second one-way valve 24, is b. The length a of the sealing member 13 is greater than the maximum distance between the side of the fastening member 11 near the gasket 12 and the side of the first liquid capsule 221 away from the second one-way valve 24, and the maximum distance between the side of the fastening member 11 near the gasket 12 and the side of the second liquid capsule 222 away from the second one-way valve 24, which is b; that is, when the cover buckle 1 is in the fastened state, the two sealing members 13 completely penetrate the first liquid capsule 221 and the second liquid capsule 222, respectively, along the direction Z from the cover buckle 1 to the cover body 2.

[0078] Through the above scheme, when the pipette adds samples to the first section 021 and the second section 022, the pipette tip is thin and light, and the first liquid capsule 221 in the pre-buried cavity 22 of the first section 021 and the second liquid capsule 222 in the pre-buried cavity 22 of the second section 022 may not be fully punctured, so that the buffer solution in the first liquid capsule 221 and the second liquid capsule 222 cannot be completely released; the length of the sealing member 13 is greater than the distance from the side of the fastener 11 close to the gasket 12 to the side of the first liquid capsule 221 or the second liquid capsule 222 away from the second one-way valve 24, so that when the cover buckle 1 is in the fastened state, the buffer solution in the first liquid capsule 221 and the second liquid capsule 222 can be further completely released, so that the sample, PCR amplifier 223 and the buffer solution can be fully mixed.

[0079] In an alternative embodiment, continue to refer to Figure 2 and Figure 6As shown, the bases of the first sheet 31 and the second sheet 32 ​​are both provided with a flow channel mechanism 34 connected to the flow channel injection hole. The number of the flow channel mechanisms 34 is at least two, and the at least two flow channel mechanisms 34 are arranged along the circumferential direction of the first sheet 31 and the second sheet 32. The length extension direction of the at least two flow channel mechanisms 34 is the radial extension direction of the first sheet 31 or the second sheet 32. Each flow channel mechanism 34 is a groove with an opening toward the cover sheet. A reaction chamber is opened on each flow channel mechanism 34, and the reaction chamber is a circular groove. Catalytic enzyme and specific primers are arranged in the circular groove.

[0080] Specifically, each of the first and second sheets 31 and 32 is provided with at least one reaction chamber 33 and a flow channel mechanism 34 corresponding in number to and connected to the reaction chamber 33. The flow channel mechanism 34 and the reaction chamber 33 are specifically located on the side of the substrate near the cover sheet. The reaction chamber 33 is a circular groove formed in the substrate. A catalytic enzyme and a specific primer are disposed within the reaction chamber 33. Both the catalytic enzyme and the specific primer are freeze-dried preparations. The catalytic enzyme and the specific primer are common knowledge in the art and will not be further explained in this embodiment. The catalytic enzyme and the specific primer within each reaction chamber 33 match and respond to each other, and the catalytic enzyme and specific primer within each reaction chamber 33 are different. Each flow channel mechanism 34 is a groove opening toward the cover sheet, and the length of each flow channel mechanism 34 extends in the radial direction of the first or second sheet 31 and 32. One end of the flow channel mechanism 34 is connected to and connected to the reaction chamber 33, and the end of the flow channel mechanism 34 away from the reaction chamber 33 is connected to the lower injection port 28 via the flow channel injection port.

[0081] Through the above scheme, the sample, PCR extender, and buffer mixed within the pre-buried cavity 22 of the first section 021 can flow through the lower injection hole 28 within the pre-buried cavity 22 of the first section 021 and the different flow channel injection holes on the first sheet 31, and then flow into different reaction chambers 33 through different flow channel mechanisms 34, thereby combining with the catalytic enzyme and specific primers for amplification. The sample, PCR extender, and buffer mixed within the pre-buried cavity 22 of the second section 022 can flow through the lower injection hole 28 within the pre-buried cavity 22 of the second section 022 and the different flow channel injection holes on the second sheet 32, and then flow into different reaction chambers 33 through different flow channel mechanisms 34, thereby combining with the catalytic enzyme and specific primers for amplification.

[0082] In an alternative embodiment, continue to refer to Figure 2 and Figure 6 As shown, along the direction Z pointing from the cover buckle 1 to the cover body 2 , the orthographic projection of the flow channel injection hole is located within the orthographic projection of the lower injection hole 28 .

[0083] Specifically, when there are more than two flow channel mechanisms 34 and flow channel injection holes, at least two flow channel injection holes on the first piece 31 are arranged along the arc length direction of the lower injection hole 28 in the pre-buried cavity 22 of the first section 021, and the diameter of the flow channel injection holes on the first piece 31 is smaller than the width of the lower injection hole 28 in the pre-buried cavity 22 of the first section 021 in the plane of the rubber ring 26; along the direction Z from the cover buckle 1 to the cover body 2, the orthographic projections of the flow channel injection holes on the first piece 31 coincide with the orthographic projections of the lower injection hole 28 in the pre-buried cavity 22 of the first section 021.

[0084] At least two flow channel injection holes on the second piece 32 are arranged along the arc length direction of the lower injection hole 28 in the pre-buried cavity 22 of the second section 022, and the diameters of the flow channel injection holes on the second piece 32 are all smaller than the width of the lower injection hole 28 in the pre-buried cavity 22 of the second section 022 in the plane of the rubber ring 26; along the direction Z from the cover buckle 1 to the cover body 2, the orthographic projections of the flow channel injection holes on the second piece 32 all coincide with the orthographic projections of the lower injection hole 28 in the pre-buried cavity 22 of the second section 022.

[0085] Through the above solution, during the centrifugation process of chip 3, the sample mixture in the pre-buried cavity 22 of the first section 021 and the pre-buried cavity 22 of the second section 022 can flow evenly into the flow channel mechanism 34 of the first chip 31 or the second chip 32. The lower sampling hole 28 and the flow channel sampling hole, which are directly connected to each other, can further improve the efficiency of the flow, thereby increasing the rate of amplification detection.

[0086] Example 2

[0087] This embodiment is an extension solution based on embodiment 1. Figure 2 and Figure 8 As shown, Figure 8 yes Figure 2 Enlarged view of point D in the middle; the fastening member 11 is provided with at least 10 arcuate grooves 15 along its circumferential direction, and the at least 10 arcuate grooves 15 are all recessed toward the central axis of the fastening member 11 and are symmetrical about the central axis of the fastening member 11, and the length direction of the arcuate groove 15 is in the direction Z along the cover buckle 1 pointing to the cover body 2;

[0088] Along the direction Z pointing from the cover buckle 1 to the cover body 2 , the orthographic projection of the arc-shaped groove 15 is tangent to the orthographic projection of the buckling ring 14 .

[0089] Specifically, the fastening member 11 is provided with at least ten arcuate grooves 15 along its circumference. The length of the arcuate grooves 15 is in the direction Z from the cover buckle 1 toward the cover body 2. The at least ten arcuate grooves 15 are arranged equidistantly along the circumference of the fastening member 11. Each arcuate groove 15 is recessed toward the central axis of the fastening member 11 and is symmetrical about the central axis of the fastening member 11. Along the direction Z from the cover buckle 1 toward the cover body 2, the arcuate grooves 15 contact the fastening ring 14 near the gasket 12, and the orthographic projection of the arcuate grooves 15 is tangent to the orthographic projection of the fastening ring 14.

[0090] Through the above solution, the grip roughness of the fastener 11 during use by the user can be increased, making it easier for the user to grip the fastener 11 and fasten it in the direction Z pointing from the cover buckle 1 to the cover body 2 .

[0091] There is a relationship of mutual coordination and functional support between the various technical features of the present invention, that is, the technical solution is not a "simple superposition" of technical features between multiple comparative documents, so the present invention does not fall under the "simple superposition" situation in Chapter 4 of Part 2 of the "Patent Examination Guidelines".

[0092] Although some specific embodiments of the present invention have been described in detail by way of examples, it should be understood by those skilled in the art that the above examples are for illustration only and are not intended to limit the scope of the present invention. It should be understood by those skilled in the art that modifications may be made to the above embodiments without departing from the scope and spirit of the present invention. The scope of the present invention is defined by the appended claims.

Claims

1. A microfluidic chip cartridge for dual-sample PCR detection, characterized in that: It includes a cover buckle, a cover body and a circular chip, wherein the cover buckle covers the cover body, and the cover body, the cover buckle and the chip are coaxially arranged; The cover body includes a hollow cylindrical cover body side wall, a circular first one-way valve, a circular second one-way valve and a circular rubber ring, the first one-way valve, the second one-way valve and the rubber ring are sequentially arranged on the inner wall of the cover body side wall along the direction from the cover buckle to the cover body, a closed cavity is formed between part of the cover body side wall, the first one-way valve and the second one-way valve, and a pre-buried cavity is formed between part of the cover body side wall, the second one-way valve and the rubber ring; along the direction from the cover buckle to the cover body, the orthographic projection of the first one-way valve, the orthographic projection of the second one-way valve and the orthographic projection of the rubber ring completely overlap; a rectangular first partition is bonded between the central axis of the first one-way valve and the central axis of the second one-way valve, and a rectangular second partition is bonded between the central axis of the second one-way valve and the central axis of the rubber ring; Along the direction from the cover buckle to the cover body, the orthographic projection of the first partition and the orthographic projection of the second partition completely overlap; the first partition and the second partition respectively divide the sealed cavity and the embedded cavity into a first section and a second section; along the direction from the cover buckle to the cover body, the first section in the sealed cavity corresponds to the first section in the embedded cavity, and the second section in the sealed cavity corresponds to the second section in the embedded cavity; Along the direction from the cover buckle to the cover body, the rubber ring is provided with an arc-shaped lower sampling hole running through the thickness thereof, and the number of the lower sampling holes is two, and the two lower sampling holes are respectively connected to the embedded cavity corresponding to the first section and the embedded cavity corresponding to the second section, and the two lower sampling holes are mirror-symmetrical with the second partition as the symmetry plane; A first liquid capsule made of polyester is bonded to the side of the second one-way valve corresponding to the first section away from the first one-way valve, and a second liquid capsule made of polyester is bonded to the side of the second one-way valve corresponding to the second section away from the first one-way valve; both the first and second liquid capsules are filled with buffer; and both the pre-buried cavity corresponding to the first section and the pre-buried cavity corresponding to the second section are provided with a PCR amplification agent. The cover buckle is clamped on the side of the first one-way valve away from the closed cavity, and comprises a hollow cylindrical buckle, a circular gasket and a conical sealing member bonded in sequence along the direction from the cover buckle to the cover body, wherein the buckle is bonded to the gasket on the side close to the gasket; The diameter of the fastener is equal to the outer diameter of the side wall of the cover body, and the fastener is fastened to the top of the side wall of the cover body; the diameter of the gasket is equal to the inner diameter of the side wall of the cover body, and it contacts and fastens with the first one-way valve inside the side wall of the cover body, and a gasket through-hole is opened on the gasket, which passes through the gasket along the direction of the cover buckle pointing to the cover body, and the number of the gasket through-holes is two, and the two gasket through-holes correspond to the first liquid capsule and the second liquid capsule respectively; the number of the sealing members is two, and the tips of the two sealing members are both facing one side of the first one-way valve, and the two sealing members are respectively inserted into the two gasket through-holes; when the sealing members are in the fastened state, the tips of the two sealing members respectively pass through the first one-way valve and the second one-way valve in sequence along the direction of the cover buckle pointing to the cover body and extend into the embedded cavity; The chip is divided into a first and a second semicircular piece with the same structure with the plane where the second partition is located as the symmetry plane. The first and the second pieces each include a circular base and a cover piece bonded to the base. The cover piece matches the base. The base is located on the side of the cover piece away from the rubber ring. The side of the cover piece away from the base is connected to the rubber ring. The cover pieces of the first and the second pieces are respectively provided with flow channel injection holes corresponding to the two lower injection holes.

2. The microfluidic chip cartridge for dual-sample PCR detection according to claim 1, characterized in that: The first one-way valve and the second one-way valve are respectively provided with corresponding circular upper injection holes. There are two upper injection holes, a first injection hole corresponding to the first section and a second injection hole corresponding to the second section. The first injection hole and the second injection hole correspond to the first liquid capsule and the second liquid capsule respectively. The first injection hole is used to pass through the sealed cavity and the embedded cavity corresponding to the first section, and the second injection hole is used to pass through the sealed cavity and the embedded cavity corresponding to the second section. The natural state of the first injection hole and the second injection hole is an elastic closed state. When the sealing members are in a buckled state, along the direction from the cover buckle to the cover body, the tip of the first sealing member sequentially passes through the first injection hole of the first one-way valve and the first injection hole of the second one-way valve and extends into the embedded cavity, and the tip of the second sealing member sequentially passes through the second injection hole of the first one-way valve and the second injection hole of the second one-way valve and extends into the embedded cavity.

3. The microfluidic chip cartridge for dual-sample PCR detection according to claim 1, characterized in that: A buckling ring is fixedly bonded to the outer side of the buckling member close to one end of the gasket and away from the sealing member; the buckling ring extends along its circumferential direction and is a hollow ring body, and its cross-section shape is hook-shaped; The side wall of the cover body is fixedly bonded with a fixing ring extending along its circumferential direction corresponding to the outer wall of the first one-way valve. The fixing ring is a hollow annular body with an inverted L-shaped cross-section. The fixing ring matches the buckling ring and is buckled to the buckling ring.

4. The microfluidic chip cartridge for dual-sample PCR detection according to claim 3, characterized in that: The length of the sealing member along the direction from the cover buckle to the cover body is a, the maximum distance between the side of the fastener close to the gasket and the side of the first liquid sac away from the second one-way valve, and the maximum distance between the side of the fastener close to the gasket and the side of the second liquid sac away from the second one-way valve are b, a>b.

5. The microfluidic chip cartridge for dual-sample PCR detection according to claim 3, characterized in that: The fastening member is provided with at least 10 arcuate grooves along its circumferential direction, and the at least 10 arcuate grooves are all recessed toward the central axis of the fastening member and are symmetrical about the central axis of the fastening member, and the length direction of the arcuate groove is along the direction of the cover buckle pointing toward the cover body; Along the direction from the cover buckle to the cover body, the orthographic projection of the arc-shaped groove is tangent to the orthographic projection of the buckling ring.

6. The microfluidic chip cartridge for dual-sample PCR detection according to claim 1, characterized in that: The bases of the first sheet and the second sheet are both provided with a flow channel mechanism connected to the flow channel sampling hole, the number of the flow channel mechanisms is at least two, and the at least two flow channel mechanisms are arranged along the circumferential direction of the first sheet and the second sheet, and the length extension direction of the at least two flow channel mechanisms is the radial extension direction of the first sheet or the second sheet, each flow channel mechanism is a groove opening toward the cover sheet, and each flow channel mechanism is provided with a reaction chamber, which is a circular groove, and a catalytic enzyme and a specific primer are arranged in the circular groove.

7. The microfluidic chip cartridge for dual-sample PCR detection according to claim 6, characterized in that: Along the direction from the cover buckle to the cover body, the orthographic projection of the flow channel injection hole is located within the orthographic projection of the lower injection hole.

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