Thin-layer sample application auxiliary device and method for jujube inspection

Abstract

The invention provides a thin-layer sample application auxiliary device and method for jujube inspection, and relates to the technical field of detection.The thin-layer sample application auxiliary device comprises an operation panel, a plurality of positioning mechanisms are installed on the operation panel, and the positioning mechanisms are used for fixing a thin-layer plate to the top face of the operation panel; an adjusting support is further installed on the operation plate, a transverse moving plate is installed on the adjusting support in a sliding mode, a lifting frame is installed on the transverse moving plate in a sliding mode, a pencil is detachably installed on the lifting frame, and extrusion barrels are arranged on the two sides of the lifting frame. The invention provides a thin-layer sample application auxiliary device and method for jujube inspection. The thin-layer sample application auxiliary device comprises an operation plate, a positioning mechanism, an adjusting support, a transverse moving plate, a lifting frame, a pencil, an extrusion container, an injector, a collaboration mechanism and a height fixing mechanism. The extrusion amount of the reagent can be accurately controlled according to the concentration of the reagent, the thickness and the material of the thin-layer plate and the like, the operation is simple and convenient, the operation amount of workers is reduced, the result comparability is ensured, quantitative analysis is facilitated, and error sources are reduced.

Description

Technical Field

[0001] The present invention relates to the field of detection technology, and in particular to a thin layer spotting auxiliary device and method for jujube inspection. Background Art

[0002] In the fields of food, medicine, etc., the quality and safety inspection of jujube is of vital importance. Thin layer chromatography, as a commonly used analytical method, has been widely used in jujube inspection due to its advantages such as simple operation, rapidity and good separation effect. Spot sampling is a key step in thin layer chromatography analysis, and its accuracy and repeatability directly affect the reliability of the analysis results.

[0003] At present, the 2015 and 2020 editions of the Chinese Pharmacopoeia both provide a method for identifying jujubes. Using thin-layer chromatography, the test sample solution and the control medicinal material solution were aspirated and spotted on the same silica gel G thin-layer plate for development and inspection. Scholars Liu Shijun, Zhang Xueyuan, etc. used thin-layer chromatography to qualitatively identify the amino acid components in jujubes in "Thin-layer Chromatographic Analysis of Amino Acid Components in Jujubes". During the operation, 1 μl of test samples 1 and 2 were respectively aspirated and spotted on the silica gel G plate for development and color development. However, the existing technology has many problems in the thin-layer spotting process of jujube inspection; traditional spotting methods, such as using glass capillary spotting, are difficult to accurately control the injection volume, resulting in the inability to standardize and accurately determine the spotting concentration, and the spotting error is large, which seriously reduces the credibility of the test results; when faced with a large number of jujube samples that need to be repeatedly spotted, manual operation is not only boring, but over time, the experimenter's fatigue increases, resulting in a decrease in operation accuracy and repeatability. Summary of the Invention

[0004] Based on the technical problems existing in the background technology, the present invention proposes a thin layer spotting auxiliary device and method for jujube inspection.

[0005] The present invention provides a thin layer sample spotting auxiliary device for jujube inspection, comprising an operating panel, on which a plurality of positioning mechanisms are mounted, and the positioning mechanisms are used to fix the thin layer plate on the top surface of the operating panel; The operation panel is also equipped with an installation and adjustment bracket, a transverse plate is slidably mounted on the adjustment bracket, a lifting frame is slidably mounted on the transverse plate, a pencil is detachably mounted on the lifting frame, an extrusion cylinder is provided on both sides of the lifting frame, a syringe is mounted on the extrusion cylinder, and a coordination mechanism is mounted on the transverse plate; When the lifting frame moves up and down on the transverse plate, the cooperative mechanism can convert the lifting motion of the lifting frame into the lifting motion of the extrusion cylinder; A height-fixing mechanism is installed on the transverse plate. When the extrusion cylinder and the syringe descend, when the end of the injection needle of the syringe is 2-3 mm away from the surface of the thin layer plate, the height-fixing mechanism fixes the descending position of the syringe, and the extrusion cylinder continues to descend and squeezes out the reagent in the syringe.

[0006] Preferably, the cooperative mechanism includes a No. 1 telescopic rod, a No. 2 telescopic rod and a rotating frame; the No. 1 telescopic rod and the No. 2 telescopic rod are arranged in parallel, and the ends of the No. 1 telescopic rod and the No. 2 telescopic rod are rotatably connected to the lifting frame and the extrusion cylinder respectively, and the lifting frame and the extrusion cylinder are always kept parallel; The second telescopic rod slides through the rotating frame, and the rotating frame is rotatably mounted on the transverse plate.

[0007] Preferably, the height-fixing mechanism comprises a sleeve block, a blocking bar and a side bar; the sleeve block is sleeved on the outer periphery of the syringe, the blocking bar is fixedly connected to one side of the transverse plate, and the side bar is fixedly connected to one side of the sleeve block; When the sleeve block descends, the sleeve block drives the side strips to abut against the blocking strips.

[0008] Preferably, the extrusion cylinder is slidably mounted on the syringe, a liquid filling port communicating with the syringe is provided on the top of the extrusion cylinder, and a sealing plug capable of sealing the liquid filling port is detachably mounted on the extrusion cylinder; A distance adjustment mechanism is provided between the extrusion cylinder and the sleeve block, and the distance adjustment mechanism can adjust the distance between the bottom end of the extrusion cylinder and the top end of the sleeve block.

[0009] Preferably, the distance adjustment mechanism includes an adjusting ring, a telescopic rod, an anti-slip block, a reset spring and an adjusting assembly; an annular adjusting groove that slides with the adjusting ring is provided in the sleeve block, one end of the telescopic rod is fixedly connected to the adjusting ring, the other end of the telescopic rod slides through the sleeve block and extends to the outside of the sleeve block, a telescopic hole that slides with the telescopic rod is provided on the extrusion cylinder, the anti-slip block is fixedly connected to the other end of the telescopic rod, and an anti-slip groove that slides with the anti-slip block is provided in the extrusion cylinder; The return spring is located in the anti-slip groove, and the two ends of the return spring abut against the anti-slip block and the inner wall of the end of the anti-slip groove respectively; The adjusting assembly is used to adjust the position of the adjusting ring in the annular adjusting groove.

[0010] Preferably, the adjustment assembly includes a movable block, a pressing rod, a positioning piece and a positioning spring; a receiving groove capable of receiving the movable block is provided on the outer circumference of the adjustment ring; one end of the pressing rod is fixedly connected to the side of the movable block; the other end of the pressing rod slides through the sleeve block and extends to the outside of the sleeve block; the sleeve block is provided with an adjustment groove that slides with the pressing rod; The positioning piece is connected to the side of the movable block close to the pressing rod, and the inner wall of the annular adjustment groove is provided with a plurality of positioning grooves adapted to the pressing rod along its axial direction; The outer periphery of the pressing rod is circumferentially connected with a boss, and a movable sliding groove that slides with the boss is opened in the sleeve block. The positioning spring is located in the movable sliding groove, and the two ends of the positioning spring are respectively against the inner wall of the end of the boss and the movable sliding groove.

[0011] Preferably, the positioning mechanism includes a supporting block and a pressing block; the supporting block is mounted on the top surface of the operating panel, and the pressing block is slidably mounted on one side of the supporting block.

[0012] Preferably, the adjustment bracket includes an electric lifting rod and a crossbeam; the electric lifting rod is slidably mounted on the operating panel, the crossbeam is mounted on the output shaft of the electric lifting rod, and the crossbeam is arranged parallel to the top surface of the operating panel.

[0013] An operating method of a thin layer spotting auxiliary device for jujube inspection is as follows: S1. Place the thin layer plate on the operating plate in an upright position, and slide the pressing block down along the side of the abutting block to press and fix the edge of the thin layer plate; S2. The thickness of the thin layer plate is detected according to the height of the pressing block, and the electric lifting rod drives the beam to adjust the height; S3, the electric lifting rod slides to the specified position on the operating panel, the extrusion cylinder descends on the crossbeam and the transverse plate moves horizontally on the crossbeam, driving the pencil to draw a line on the thin layer plate; S4. Adjusting the distance between the extrusion cylinder and the syringe according to the concentrations of the contrast reagent and the jujube extract reagent; S5. Add the contrast reagent and the jujube extract reagent into two syringes respectively; S6, the extrusion cylinder moves upward on the beam, and the two syringes descend synchronously, and the contrast reagent and jujube extract reagent in the two syringes are squeezed at the same pressure at the same time; S7. Blow the sample dry.

[0014] Preferably, in S3, the line drawn on the thin layer plate is 1.5 cm away from one side of the thin layer plate. The thin layer sample spotting auxiliary device and method for jujube inspection proposed in the present invention have the following beneficial effects: through the provided operating panel, positioning mechanism, adjustment bracket, transverse plate, lifting frame, pencil, extrusion cylinder, syringe, coordination mechanism and height-fixing mechanism; the extrusion amount of the reagent can be accurately controlled according to the concentration of the reagent, the thickness and material of the thin layer plate, etc., the operation is simple and convenient, the operation workload of the staff is reduced, the comparability of the results is ensured, quantitative analysis is facilitated and the source of error is reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of the overall structure of a thin layer spotting auxiliary device for jujube inspection proposed by the present invention; Figure 2 This is a schematic diagram of the structure of the transverse moving plate, lifting frame, syringe, etc. in the thin layer spotting auxiliary device for jujube inspection proposed by the present invention; Figure 3 This is a cross-sectional view of the structure of the extrusion cylinder and syringe in a thin layer sample spotting auxiliary device for jujube inspection proposed by the present invention; Figure 4 This is a top-down cross-sectional view of the position of the adjustment ring in the sleeve block in the thin-layer sample spotting auxiliary device for jujube inspection proposed by the present invention; Figure 5 This is a schematic structural diagram of the adjustment ring, telescopic rod and anti-fall-off block in a thin layer sample spotting auxiliary device for jujube inspection proposed by the present invention; Figure 6 This is a schematic diagram of the structure of the pencil, squeeze tube, syringe and cooperative mechanism in a thin layer spotting auxiliary device for jujube inspection proposed by the present invention; Figure 7 This is a schematic diagram of the position of the blocking strip on the transverse plate in the thin layer spotting auxiliary device for jujube inspection proposed by the present invention; Figure 8 This is a schematic structural diagram of the movable block, pressing rod and positioning piece in a thin layer sample spotting auxiliary device for jujube inspection proposed by the present invention; Figure 9 The figure is a cross-sectional view of the positioning mechanism in a thin layer spotting auxiliary device for jujube inspection proposed by the present invention.

[0016] In the figure: 1. operating panel; 2. transverse plate; 3. lifting frame; 4. pencil; 5. extrusion cylinder; 6. syringe; 7. telescopic rod No. 1; 8. telescopic rod No. 2; 9. rotating frame; 10. sleeve block; 11. blocking bar; 12. side bar; 13. sealing plug; 14. adjusting ring; 15. telescopic rod; 16. anti-slip block; 17. reset spring; 18. movable block; 19. pressing rod; 20. positioning piece; 21. positioning spring; 22. supporting block; 23. pressing block; 24. electric lifting rod; 25. crossbeam; 26. storage slot. DETAILED DESCRIPTION

[0017] Reference Figures 1-9The present invention proposes a thin layer sample spotting auxiliary device for jujube inspection, comprising an operating panel 1, on which a plurality of positioning mechanisms are installed, the positioning mechanisms being used to fix the thin layer plate on the top surface of the operating panel 1, the positioning mechanisms comprising abutting blocks 22 and pressing blocks 23, the butting blocks 22 being installed on the top surface of the operating panel 1, the pressing blocks 23 being slidably installed on one side of the butting blocks 22, the positioning mechanisms having three, one of which is fixed on the operating panel, to place one side of the thin layer plate against the butting blocks 22, and then the pressing blocks 23 are pressed against the thin layer plate by the No. 1 electric slide rail, and then the two positioning mechanisms are placed on both sides of the thin layer plate to fix both sides of the thin layer plate, thereby ensuring that the thin layer plate will not move during the process of drawing lines and spotting, ensuring The accuracy of the drawing position and the dot position is detected by the displacement sensor to detect the height of the pressing block 23 moving downward; the operating panel 1 is also equipped with an installation adjustment bracket, which includes an electric lifting rod 24 and a crossbeam 25; the crossbeam 25 is installed on the output shaft of the electric lifting rod 24, and the crossbeam 25 is arranged parallel to the top surface of the operating panel 1. The adjustment bracket is slidably mounted with a transverse plate 2 (the moving direction of the transverse plate 2 is parallel to the top surface of the operating panel 1), and the lifting frame 3 is slidably mounted on the transverse plate 2. The lifting frame 3 is detachably mounted with a pencil 4. When the lifting frame 3 is raised and lowered, the pencil 4 will be driven to rise and fall synchronously. The lateral movement of the transverse plate 2 allows the pencil 4 to draw a baseline on the thin layer plate. Extrusion cylinders 5 are provided on both sides of the lifting frame 3. , a syringe 6 is installed on the extrusion cylinder 5, and a coordination mechanism is installed on the transverse plate 2. In addition, the tip of the pencil 4 and the ends of the injection needles of the two syringes 6 are on the same plane (when the tip of the pencil 4 and the ends of the injection needles of the syringes 6 are at the same height, the three are on the same straight line). When the lifting frame 3 moves up and down on the transverse plate 2, the coordination mechanism can convert the lifting movement of the lifting frame 3 into the lifting and lowering movement of the extrusion cylinder 5. A height-fixing mechanism is installed on the transverse plate 2. When the extrusion cylinder 5 and the syringe 6 are lowered, the end of the injection needle of the syringe 6 is 2-3mm away from the surface of the thin layer plate (to prevent the injection needle from damaging the surface of the thin layer plate), the height-fixing mechanism fixes the lowered position of the syringe 6, and the extrusion cylinder 5 The two syringes 6 continue to descend and squeeze out the reagent in the syringe 6. When spotting is required, the lifting frame 3 is raised. Through the coordinated transmission action of the two cooperative mechanisms, the two syringes 6 are synchronously lowered and suspended by the height-fixing mechanism. Then, the two syringes 6 are squeezed synchronously to ensure that the squeezing amount of the comparison reagent and the jujube extract reagent is consistent, which can ensure the comparability of the results (thin layer chromatography performs qualitative analysis by comparing the position and color of the spots of the sample and the reference substance. The same squeezing amount can make the initial content of the comparison reagent and the test reagent on the thin layer plate consistent. After the same development process, if spots appear in the same position on both, it can be more accurately judged that the test sample contains the same component as the comparison reagent);When performing quantitative analysis, it is often necessary to plot a standard curve. This ensures that the extrusion volumes of the comparison reagent and the test reagent are identical, ensuring that the concentration-spot intensity relationship of the standard (comparison reagent) is accurately reflected on the standard curve. This allows the target component content in the sample to be accurately calculated based on the test reagent spot intensity. Identical extrusion volumes prevent the effects of spot size and color variations caused by different spotting volumes on quantitative results, improving the precision and accuracy of quantitative analysis and bringing test results closer to the true value. Furthermore, this reduces the number of steps and time required for spotting, improving experimental efficiency and minimizing the risk of errors and operational errors caused by repeated adjustments to the spotting volume. This auxiliary device utilizes a mechanical transmission method, resulting in a simpler structure and more convenient operation. This simplifies manual labor and eliminates the need for expensive automated spotting machines.

[0018] like Figure 1 、 Figure 2 and Figure 6 As shown in, the collaborative mechanism includes a No. 1 telescopic rod 7, a No. 2 telescopic rod 8 and a rotating frame 9; the No. 1 telescopic rod 7 and the No. 2 telescopic rod 8 are arranged in parallel, and the lengths of the No. 1 telescopic rod 7 and the No. 2 telescopic rod 8 can be adjusted as needed. It is necessary to ensure that the lengths of the No. 1 telescopic rod 7 and the No. 2 telescopic rod 8 are the same. During operation, the lengths of the No. 1 telescopic rod 7 and the No. 2 telescopic rod 8 will not change. The two ends of the No. 1 telescopic rod 7 and the No. 2 telescopic rod 8 are rotatably connected to the lifting frame 3 and the extrusion cylinder 5 respectively, and the lifting frame 3 and the extrusion cylinder 5 always remain parallel. The No. 2 telescopic rod 8 slides through the rotating frame 9, and the rotating frame 9 is rotatably installed on the transverse plate 2. The lifting frame 3, the No. 1 telescopic rod 7, the No. 2 telescopic rod 8 and the extrusion cylinder 5 form a parallelogram structure. When the lifting frame 3 moves up and down, the two extrusion cylinders 5 move up and down synchronously. Due to the characteristics of the parallelogram, the two extrusion cylinders 5 and the syringe 6 can always remain perpendicular to the thin layer plate.

[0019] like Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 and Figure 7 As shown in , the height-fixing mechanism includes a sleeve block 10, a blocking bar 11 and a side bar 12; the sleeve block 10 is sleeved on the outer periphery of the syringe 6, the blocking bar 11 is fixedly connected to one side of the transverse plate 2, and the side bar 12 is fixedly connected to one side of the sleeve block 10. When the sleeve block 10 descends, the sleeve block 10 drives the side bar 12 to abut against the blocking bar 11. When the syringe 6 descends, the sleeve block 10 descends synchronously until the side bar 12 abuts against the blocking bar 11. The sleeve block 10 and the syringe 6 stop descending, and the two extrusion cylinders 5 continue to descend synchronously to squeeze out the reagent in the syringe 6. Since the two extrusion cylinders 5 descend synchronously, the two reagents in the two syringes 6 can be squeezed out, and the extrusion amount is also consistent, thereby ensuring the comparability of the results.

[0020] like Figure 3 , pictures and Figure 5 As shown in the figure, the extrusion cylinder 5 is slidably mounted on the syringe 6, and a liquid filling port connected to the syringe 6 is provided on the top of the extrusion cylinder 5. A sealing plug 13 capable of sealing the liquid filling port is detachably installed on the extrusion cylinder 5. A distance adjustment mechanism is provided between the extrusion cylinder 5 and the sleeve block 10. The distance adjustment mechanism can adjust the distance between the bottom end of the extrusion cylinder 5 and the top end of the sleeve block 10. In actual operation, the spot needs to be clear and the extrusion amount should be sufficient so that the spot formed by the sample on the thin layer plate has sufficient substance concentration and can be clearly seen after expansion and color development for easy observation and analysis. If the extrusion amount is too little, the spot is too light or too small, and it may be impossible to accurately judge the presence and position of the component in the sample; at the same time, the extrusion amount should not be too much, otherwise the spot will be If the sample spot is too large, it is easy for the spots to overlap with each other during the expansion process, affecting the separation effect. At the same time, too much sample may also cause spot tailing, making the spot shape irregular, difficult to accurately measure its relative shift value (Rf value), and interfering with the judgment of the results. When spotting, it is also necessary to consider factors such as the concentration of the sample, the properties of the thin layer plate, and the purpose of analysis to control the optimal extrusion amount of the reagent. Since the extrusion cylinder 5 is sleeved on the syringe 6, the space in the syringe 6 is reduced, and the reagent is squeezed out by air pressure. However, the distance that the extrusion cylinder 5 is sleeved on the syringe 6 is the distance from the bottom of the extrusion cylinder 5 to the top of the sleeve block 10. Therefore, the extrusion amount of the reagent can be controlled by adjusting the distance between the bottom end of the extrusion cylinder 5 and the top of the sleeve block 10.

[0021] like Figure 3 、 Figure 4 、 Figure 6 and Figure 8As shown in , the distance adjustment mechanism includes an adjusting ring 14, a telescopic rod 15, an anti-falling block 16, a reset spring 17 and an adjusting assembly; an annular adjusting groove that slides with the adjusting ring 14 is provided in the sleeve block 10, one end of the telescopic rod 15 is fixedly connected to the adjusting ring 14, and the other end of the telescopic rod 15 slides through the sleeve block 10 and extends to the outside of the sleeve block 10, a telescopic hole that slides with the telescopic rod 15 is provided on the extrusion cylinder 5, the anti-falling block 16 is fixedly connected to the other end of the telescopic rod 15, an anti-falling groove that slides with the anti-falling block 16 is provided in the extrusion cylinder 5, and the reset spring 17 is located at the anti-falling groove. In the slip groove, the two ends of the return spring 17 respectively abut against the anti-slip block 16 and the inner wall of the end of the anti-slip groove. The adjusting assembly is used to adjust the position of the adjusting ring 14 in the annular adjusting groove. The adjusting assembly includes a movable block 18, a pressing rod 19, a positioning piece 20 and a positioning spring 21; the outer periphery of the adjusting ring 14 is provided with a receiving groove 26 that can accommodate the movable block 18, one end of the pressing rod 19 is fixedly connected to the side of the movable block 18, and the other end of the pressing rod 19 slides through the sleeve block 10 and extends to the outside of the sleeve block 10, and the sleeve block 10 is provided with an adjusting groove that slides with the pressing rod 19 The positioning piece 20 is connected to the side of the movable block 18 close to the pressing rod 19. The inner wall of the annular adjustment groove is provided with a plurality of positioning grooves adapted to the pressing rod 19 along its axial direction. The outer periphery of the pressing rod 19 is circumferentially connected with a boss. A movable slide groove that slides with the boss is provided in the sleeve block 10. The positioning spring 21 is located in the movable slide groove, and the two ends of the positioning spring 21 respectively abut against the boss and the inner wall of the end of the movable slide groove. When adjusting the distance between the bottom of the extrusion cylinder 5 and the top of the sleeve block 10, the staff pinches the pressing rods 19 on both sides of the sleeve block 10, and the pressing rods 19 drive the movable block 18 drives the positioning piece 20 to retract into the receiving groove 26, and then slides the pressing rod 19. The pressing rod 19 drives the adjusting ring 14 to slide in the annular adjusting groove. When it is adjusted to the appropriate position (the scale value is engraved on the sleeve block 10), under the rebound action of the positioning spring 21, the boss and the pressing rod 19 are pushed to slide and reset. The pressing rod 19 drives the movable block 18 and the positioning piece 20 to slide and reset. The positioning piece 20 is inserted into the positioning groove at the corresponding position (so that the length of the telescopic rod 15 exposed to the outside of the sleeve block 10 changes), completing the distance adjustment from the bottom of the extrusion cylinder 5 to the top of the sleeve block 10.

[0022] The specific operation method is as follows: S1. Place the thin layer plate in an upright position on the operating plate 1. The pressing block 23 slides down along the side of the abutting block 22 to press and fix the edge of the thin layer plate. S2, the thickness of the thin layer plate is detected according to the height of the pressing block 23, and the electric lifting rod 24 drives the crossbeam 25 to adjust the height; S3. The electric lifting rod 24 slides to the designated position on the operating panel 1. The extrusion cylinder 5 descends on the crossbeam 25. At the same time, the transverse plate 2 moves laterally on the crossbeam 25. The pencil 4 is driven to draw a baseline on the thin layer plate. The baseline is located 1.5 cm away from one side of the thin layer plate. S4, adjusting the distance between the extrusion cylinder 5 and the syringe 6 according to the concentrations of the contrast reagent and the jujube extract reagent; S5, adding the contrast reagent and the jujube extract reagent into two syringes 6 respectively; S6, the extrusion cylinder 5 moves upward on the crossbeam 25, and the two syringes 6 descend synchronously, and simultaneously squeeze the contrast reagent and jujube extract reagent in the two syringes 6 with the same pressure; S7. Do not use hot air when drying the samples.

[0023] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. A thin layer spotting auxiliary device for jujube inspection, characterized in that: It comprises an operating panel (1), wherein a plurality of positioning mechanisms are mounted on the operating panel (1), and the positioning mechanisms are used to fix the thin layer plate on the top surface of the operating panel (1); The operating panel (1) is further provided with an installation adjustment bracket, a transverse plate (2) is slidably installed on the adjustment bracket, a lifting frame (3) is slidably installed on the transverse plate (2), a pencil (4) is detachably installed on the lifting frame (3), an extrusion cylinder (5) is provided on both sides of the lifting frame (3), a syringe (6) is installed on the extrusion cylinder (5), and a coordination mechanism is installed on the transverse plate (2); When the lifting frame (3) moves up and down on the transverse plate (2), the cooperative mechanism can convert the lifting movement of the lifting frame (3) into the lifting and lowering movement of the extrusion cylinder (5); A height-fixing mechanism is installed on the transverse plate (2). When the extrusion cylinder (5) and the syringe (6) descend, when the end of the injection needle of the syringe (6) is 2-3 mm away from the surface of the thin layer plate, the height-fixing mechanism fixes the descending position of the syringe (6), and at the same time, the extrusion cylinder (5) continues to descend and squeezes out the reagent in the syringe (6).

2. A thin layer spotting auxiliary device for jujube inspection according to claim 1, characterized in that: The cooperative mechanism comprises a No. 1 telescopic rod (7), a No. 2 telescopic rod (8) and a rotating frame (9); the No. 1 telescopic rod (7) and the No. 2 telescopic rod (8) are arranged in parallel, and the two ends of the No. 1 telescopic rod (7) and the No. 2 telescopic rod (8) are rotatably connected to the lifting frame (3) and the extrusion cylinder (5), respectively, and the lifting frame (3) and the extrusion cylinder (5) always remain parallel; The second telescopic rod (8) slides through the rotating frame (9), and the rotating frame (9) is rotatably mounted on the transverse plate (2).

3. A thin layer spotting auxiliary device for jujube inspection according to claim 2, characterized in that: The height-fixing mechanism comprises a sleeve block (10), a blocking bar (11) and a side bar (12); the sleeve block (10) is sleeved on the outer periphery of the syringe (6), the blocking bar (11) is fixedly connected to one side of the transverse plate (2), and the side bar (12) is fixedly connected to one side of the sleeve block (10); When the sleeve block (10) descends, the sleeve block (10) drives the side strip (12) to abut against the blocking strip (11).

4. A thin layer spotting auxiliary device for jujube inspection according to claim 3, characterized in that: The extrusion cylinder (5) is slidably mounted on the syringe (6); a liquid filling port communicating with the syringe (6) is provided on the top of the extrusion cylinder (5); and a sealing plug (13) capable of sealing the liquid filling port is detachably mounted on the extrusion cylinder (5); A distance adjustment mechanism is provided between the extrusion cylinder (5) and the sleeve block (10), and the distance adjustment mechanism is capable of adjusting the distance between the bottom end of the extrusion cylinder (5) and the top end of the sleeve block (10).

5. A thin layer spotting auxiliary device for jujube inspection according to claim 4, characterized in that: The distance adjustment mechanism includes an adjusting ring (14), a telescopic rod (15), an anti-slip block (16), a return spring (17) and an adjusting assembly; an annular adjusting groove is provided in the sleeve block (10) and is slidably matched with the adjusting ring (14); one end of the telescopic rod (15) is fixedly connected to the adjusting ring (14); the other end of the telescopic rod (15) slides through the sleeve block (10) and extends to the outside of the sleeve block (10); a telescopic hole is provided on the extrusion cylinder (5) and is slidably matched with the telescopic rod (15); the anti-slip block (16) is fixedly connected to the other end of the telescopic rod (15); and an anti-slip groove is provided in the extrusion cylinder (5) and is slidably matched with the anti-slip block (16); The return spring (17) is located in the anti-slip groove, and the two ends of the return spring (17) respectively abut against the anti-slip block (16) and the inner wall of the end of the anti-slip groove; The adjustment assembly is used to adjust the position of the adjustment ring (14) in the annular adjustment groove.

6. A thin layer spotting auxiliary device for jujube inspection according to claim 5, characterized in that: The adjustment assembly includes a movable block (18), a pressing rod (19), a positioning piece (20) and a positioning spring (21); a receiving groove (26) capable of receiving the movable block (18) is provided on the outer periphery of the adjustment ring (14); one end of the pressing rod (19) is fixedly connected to the side of the movable block (18); the other end of the pressing rod (19) slides through the sleeve block (10) and extends to the outside of the sleeve block (10); the sleeve block (10) is provided with an adjustment groove that slides with the pressing rod (19); The positioning piece (20) is connected to a side of the movable block (18) close to the pressing rod (19), and the inner wall of the annular adjustment groove is provided with a plurality of positioning grooves adapted to the pressing rod (19) along its axial direction; The outer periphery of the pressing rod (19) is circumferentially connected to a boss, and a movable slide groove is provided in the sleeve block (10) for sliding cooperation with the boss. The positioning spring (21) is located in the movable slide groove, and the two ends of the positioning spring (21) respectively abut against the inner wall of the end of the boss and the movable slide groove.

7. The thin layer spotting auxiliary device for jujube inspection according to claim 1, characterized in that: The positioning mechanism comprises a supporting block (22) and a pressing block (23); the supporting block (22) is mounted on the top surface of the operating panel (1), and the pressing block (23) is slidably mounted on one side of the supporting block (22).

8. The thin layer spotting auxiliary device for jujube inspection according to claim 1, characterized in that: The adjustment bracket includes an electric lifting rod (24) and a crossbeam (25); the electric lifting rod (24) is slidably mounted on the operating panel (1), the crossbeam (25) is mounted on the output shaft of the electric lifting rod (24), and the crossbeam (25) is arranged parallel to the top surface of the operating panel (1).

9. An operating method of a thin layer sample spotting auxiliary device for jujube inspection, using the thin layer sample spotting auxiliary device for jujube inspection according to any one of claims 1 to 8, characterized in that: Here’s how to do it: S1. Place the thin layer plate in an upright position on the operating plate (1), and slide the pressing block (23) down on the side of the abutting block (22) to press and fix the edge of the thin layer plate; S2, the thickness of the thin layer plate is detected according to the height of the pressing block (23) descending, and the height is adjusted by driving the crossbeam (25) by the electric lifting rod (24); S3, the electric lifting rod (24) slides to a specified position on the operating plate (1), the extrusion cylinder (5) descends on the crossbeam (25) and the transverse plate (2) moves laterally on the crossbeam (25), driving the pencil (4) to draw a line on the thin layer plate; S4, adjusting the distance between the extrusion cylinder (5) and the syringe (6) according to the concentrations of the contrast reagent and the jujube extract reagent; S5. Add the contrast reagent and the jujube extract reagent into two syringes (6) respectively; S6, the extrusion cylinder (5) moves upward on the crossbeam (25), and the two syringes (6) descend synchronously, and the contrast reagent and the jujube extract reagent in the two syringes (6) are squeezed at the same pressure at the same time; S7. Blow the sample dry.

10. The method for operating the thin layer sample spotting auxiliary device for jujube inspection according to claim 9, characterized in that: In S3, the line drawn on the thin layer plate is 1.5 cm away from one side of the thin layer plate.

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