Preparation formula of combined tea by utilizing multiple traditional Chinese medicinal materials

Abstract

The invention discloses a preparation formula of combined tea prepared from multiple traditional Chinese medicinal materials, and relates to the technical field of traditional Chinese medicinal tea substituteds.The combined tea is prepared from, by weight, 15-20 parts of eucommia ulmoides, 12-18 parts of the root of kudzu vine, 10-15 parts of apocynum venetum, 8-12 parts of hawthorn, 8-12 parts of semen cassiae, 6-10 parts of corn stigma, 2-4 parts of liquorice, 0.5-1 part of stevia rebaudiana, 3-5 parts of lophatherum gracile and 100 parts of tea leaves. According to the multi-medicinal material combined tea, the medicinal materials such as eucommia ulmoides and radix puerariae are scientifically proportioned, then effective components of the traditional Chinese medicinal materials are accurately extracted, and then filtering and purifying are performed to obtain the combined tea. The combined tea realizes multi-target collaborative blood pressure reduction, efficient component retention and taste optimization. The combined tea can inhibit angiotensin converting enzyme, improve the vascular endothelial function and remove astringent substances in a targeted manner, and has the effects of reducing blood pressure and conditioning and being good in drinking suitability.

Description

Technical Field

[0001] The invention relates to the technical field of Chinese medicinal tea substitutes, and in particular to a preparation formula for tea composed of multiple Chinese medicinal materials. Background Art

[0002] A combination of multiple Chinese medicinal materials tea is a health-preserving tea based on the theory of traditional Chinese medicine. It mixes multiple Chinese medicinal materials according to specific compatibility principles and is drunk after brewing or decocting. Its core is to utilize the synergistic effects of the properties, flavors, meridians and effects of different medicinal materials to regulate the balance of yin and yang in the human body, improve physical fitness or assist in regulating diseases. This type of tea originated from the "tea substitute" in traditional Chinese medicine. Chinese medicine tea substitute is a traditional dosage form in my country. It is guided by the theoretical principles of Chinese medicine, methods, prescriptions and medicines, and is based on the judgment of the condition based on the combination of syndrome differentiation and disease differentiation. It is a dosage form composed of selected medicines and tea (or without tea) for the prevention and treatment of diseases, post-illness conditioning or simply for health care.

[0003] Hypertension (also known as high blood pressure) is a condition characterized by persistently higher-than-normal pressure exerted by blood against blood vessel walls. Typical symptoms of hypertension include headaches, fatigue or restlessness, arrhythmias, palpitations, and tinnitus. However, many hypertensive patients may develop other complications, such as stroke, blurred vision, loss of consciousness, and amnesia, without experiencing any symptoms. This is why hypertension is often called a "silent killer."

[0004] The current traditional Chinese herbal tea substitutes for lowering blood pressure generally use a single chlorogenic acid ingredient to assist in lowering high blood pressure. This single ingredient makes the Chinese herbal tea substitutes lack comprehensive regulation of related factors such as endothelial function and blood lipid metabolism of hypertension. In addition, traditional Chinese herbal tea substitutes are generally made by crushing and grinding the Chinese medicine and then mixing it with tea leaves or making tea alone. It is difficult to absorb the effective ingredients in them through brewing, which makes the consumption effect of Chinese herbal tea substitutes poor. Summary of the Invention

[0005] In view of the deficiencies in the prior art, the present invention provides a preparation formula for a combination tea using multiple Chinese medicinal materials, which solves the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention is implemented through the following technical solutions: a preparation formula for a combination tea using multiple Chinese medicinal materials, wherein the combination tea comprises the following raw materials in parts by weight:

[0007] 15-20 parts of Eucommia ulmoides, 12-18 parts of Pueraria root, 10-15 parts of Apocynum venetum, 8-12 parts of Hawthorn, 8-12 parts of Cassia seed, 6-10 parts of Corn silk, 2-4 parts of Licorice root, 0.5-1 part of Stevia rebaudiana, 3-5 parts of Lophatherum gracile and 100 parts of tea.

[0008] Preferably, the active ingredient content standards of each medicinal material in the raw materials are: eucommia flavonoids ≥10%, puerarin ≥8%, apocynin ≥1.5%, hyperoside ≥0.8% and bamboo leaf flavonoids ≥3%, and the tea is one of black tea or green tea.

[0009] Preferably, the stevia is a natural sweetener, which is 200-300 times sweeter than ordinary sucrose and contains no calories.

[0010] A method for preparing a combination tea using multiple Chinese medicinal materials is applied to the preparation formula of the combination tea using multiple Chinese medicinal materials. The preparation method of the combination tea specifically comprises the following steps:

[0011] S1, put Eucommia ulmoides and Apocynum venetum into a vibrating screen, vibrate the screen to remove impurities, then cut the sieved Eucommia ulmoides and Apocynum venetum into thin slices, then put Pueraria lobata, Hawthorn, Cassia seed, Corn silk and Bamboo leaf into a crushing device and grind them into 80-100 mesh powder, liquorice and stevia are crushed into 100-120 mesh fine powder and 200 mesh ultrafine powder, respectively;

[0012] S2, prepare three-stage countercurrent extraction tank, eucommia ulmoides and apocynum venetum slices and ethanol are put into the first stage extraction tank according to 1:8, eucommia ulmoides, apocynum venetum slices and ethanol mixture enter the second stage extraction tank after centrifuge separation, ethanol is added to the second stage extraction tank, according to the mixture and ethanol 1:6 mixing, eucommia ulmoides, apocynum venetum slices and ethanol mixture enter the third stage extraction tank after centrifuge separation, then add ethanol to the third stage extraction tank, according to the mixture and ethanol 1:4 mixing, then filter the mixture in the extraction tank to obtain an extract;

[0013] S3, adding the crushed kudzu root, hawthorn, cassia seed, corn silk and light bamboo leaf powder into the extraction tank, adding cellulase and pectinase, after enzymatic hydrolysis, adding 8-15 times the volume of the medicinal materials into water for ultrasonic extraction, and collecting the water extract after filtration;

[0014] S4. The extract and the aqueous extract are combined, first filtered through a ceramic membrane to remove tannin and protein macromolecular impurities, and the filtrate is separated by a molecular imprinting membrane using aucubin as a template molecule to target and intercept astringent substances, so that the removal rate of aucubin is ≥90% while retaining ≥85% of the flavonoid active ingredients, thereby obtaining a refined liquid extracted from the medicinal material;

[0015] S5. The refined liquid, licorice powder and stevia powder are mixed in a ratio of 1:0.5:0.1, transferred to a vacuum concentration tank, concentrated under vacuum conditions, and the concentrate is dried into granules in a drying tower. The granules are then mixed with tea leaves in a ratio of 1:1, and packaged in non-woven tea bags at a ratio of 6 g / bag. The bags are then wrapped with aluminum foil to prevent moisture, thereby obtaining tea bags of Chinese herbal medicine combination tea.

[0016] Preferably, the three-stage solvent ethanol concentrations of the three-stage countercurrent extraction tank are: 95% ethanol is added in the first stage, 70% ethanol is added in the second stage, and 50% ethanol is added in the third stage;

[0017] The extraction time of each stage of the three-stage countercurrent extraction tank is: the first stage extraction time is 60 minutes, the second stage extraction time is 40 minutes, and the third stage extraction time is 30 minutes.

[0018] Preferably, the extraction tank is continuously stirred during the enzymatic hydrolysis process in S3 at a stirring speed of 50-80 r / min, and the temperature of the ultrasonically extracted liquid is controlled at a constant temperature of 50-60° C.

[0019] Preferably, the transmembrane pressure difference of the ceramic membrane filtration in S4 is 0.3-0.4 MPa, the membrane surface flow rate is 3-5 m / s, and the filtration temperature is 20-40° C. to avoid degradation of the active ingredients.

[0020] Preferably, the method for preparing the molecularly imprinted membrane comprises the following steps:

[0021] S4.1. Prepare aucubin, acetonitrile, acrylamide, ethylene glycol dimethacrylate, initiator azobisisobutyronitrile, and polyacrylonitrile-based membrane raw materials, mix aucubin with the acetonitrile solution, then add acrylamide and stir to form a composite solution;

[0022] S4.2, adding ethylene glycol dimethacrylate and initiator azobisisobutyronitrile to the mixture, and then degassing the mixture by ultrasonication for 10 to 20 minutes to remove oxygen to obtain a prepolymer solution;

[0023] S4.3. Immerse the polyacrylonitrile-based membrane in the prepolymerization solution and react in a constant temperature water bath at 60-70°C under nitrogen protection for 12 to 24 hours. After the reaction is completed, take out the membrane and extract it with a methanol / acetic acid mixed solution for 24 to 48 hours to thoroughly elute the template molecules. Finally, rinse the membrane with deionized water until it is neutral and vacuum dry to obtain a molecular imprinting membrane.

[0024] Preferably, the extract content of the tea bag is ≥45%, and the proportion of water-soluble components in the tea bag is ≥70%.

[0025] Preferably, the vacuum concentration tank in S5 is switched to a vacuum degree of 80-100 kPa after maintaining normal pressure for 30-60 seconds during concentration, and the vacuum state is maintained for 30-60 seconds, and the above switching cycle process is repeated until the refined liquid, licorice powder and stevia powder are concentrated to meet the standards.

[0026] The present invention provides a preparation formula for a tea combination using multiple Chinese medicinal materials. It has the following beneficial effects:

[0027] (1) This application prepares a Chinese medicine tea substitute by combining a variety of Chinese medicinal materials, and selects Chinese medicinal materials with clear antihypertensive activity such as Eucommia ulmoides, Pueraria lobata, and Apocynum venetum, and adds light bamboo leaves and tea ingredients. Among them, Eucommia ulmoides and Pueraria lobata exert direct antihypertensive effects by inhibiting angiotensin-converting enzyme and dilating peripheral blood vessels. Apocynum venetum and Crataegus pinnatifida synergistically regulate blood lipid metabolism and block the vicious cycle of hypertension and hyperlipidemia. Corn silk and Cassia seed reduce blood volume load by diuresis and moisture absorption. Bamboo leaf flavonoids in light bamboo leaves can improve microcirculation and assist in inhibiting ACE activity. By complementing the ingredients of Chinese medicinal materials, a multi-pathway regulation system is formed. Compared with a single medicinal material or traditional antihypertensive tea, it achieves two-way regulation of systolic and diastolic blood pressure, and improves dyslipidemia and microcirculation disorders associated with hypertension. Long-term consumption by people with hypertension can achieve long-term auxiliary conditioning, delay the process of vascular remodeling, and reduce the risk of cardiovascular and cerebrovascular complications.

[0028] (2) This application adopts different extraction schemes for a variety of Chinese medicinal materials. The fat-soluble medicinal materials of Eucommia ulmoides and Apocynum venetum are extracted by alcohol extraction to achieve the precise extraction of fat-soluble active ingredients such as chlorogenic acid, Eucommia ulmoides flavonoids, and Apocynum venetum glycosides. At the same time, the water-soluble medicinal materials of Pueraria lobata, Crataegus pinnatifida, Cassia seed, Corn silk and Bamboo leaves are extracted by enzymatic hydrolysis and ultrasonic extraction. The plant cell wall structure is destroyed by cellulase and pectinase, and the dissolution of effective ingredients is accelerated by ultrasonic cavitation effect, which significantly improves the extraction efficiency of polar components such as puerarin and hyperoside. Compared with traditional decoction or simple crushing process, this extraction method can target the component characteristics of different medicinal materials, avoid the degradation of heat-sensitive components caused by high temperature, and break through the dissolution limit of medicinal materials with dense cell walls, so that the extraction rate and retention rate of various active ingredients are significantly improved, so that the antihypertensive, lipid-regulating and vascular protection components in tea can be absorbed and utilized by the human body with higher bioavailability.

[0029] (3) The present application adds a natural sweetener, stevia, to the combination tea. Stevia has a sweetness far exceeding that of ordinary sucrose, does not contain calories, is not likely to cause blood sugar fluctuations, and is suitable for use by more people. At the same time, in the preparation process, a molecular imprinting membrane is used to separate specific astringent substances such as aucubin, and these substances that affect the taste are removed by targeted interception, while having little effect on active ingredients such as flavonoids. Therefore, the taste is accurately optimized without affecting the efficacy of the medicine. In addition, the combination of Chinese herbal medicine extracts and tea leaves not only enriches the aroma level of the tea soup, but its natural tea polyphenols and amino acid components can also form a flavor synergy with the Chinese herbal medicine extracts. After brewing, the combination tea has a bright soup color, a fresh aroma, and a sweet taste. It not only retains the natural herbal flavor of Chinese herbal medicines, but also has the palatability of traditional tea drinks, further enhancing the drinking experience. DETAILED DESCRIPTION

[0030] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0031] Example 1

[0032] The present invention provides a preparation formula for a combination tea using multiple Chinese medicinal materials. To achieve the above purpose, the present invention is implemented through the following technical solutions: A preparation formula for a combination tea using multiple Chinese medicinal materials, the combination tea comprising the following raw materials in parts by weight:

[0033] 15-20 parts of Eucommia ulmoides, 12-18 parts of Pueraria root, 10-15 parts of Apocynum venetum, 8-12 parts of Hawthorn, 8-12 parts of Cassia seed, 6-10 parts of Corn silk, 2-4 parts of Licorice root, 0.5-1 part of Stevia rebaudiana, 3-5 parts of Lophatherum gracile and 100 parts of tea.

[0034] Prepared according to the following raw materials in parts by weight:

[0035] 18 parts of Eucommia ulmoides, 15 parts of Pueraria root, 12 parts of Apocynum venetum, 10 parts of Crataegus pinnatifida, 10 parts of Cassia seed, 8 parts of Corn silk, 3 parts of Licorice root, 0.8 parts of Stevia rebaudiana, 4 parts of Bambusa chinensis leaves, and 100 parts of Green tea.

[0036] The preparation method of the combination tea specifically comprises the following steps:

[0037] S1, put Eucommia ulmoides and Apocynum venetum into a vibrating screen to remove impurities, then cut the sieved Eucommia ulmoides and Apocynum venetum into 1-3 mm slices, then put Pueraria lobata, Hawthorn, Cassia seed, Corn silk and Bamboo leaf into a crushing device and grind them into 80-100 mesh powder, and grind licorice and stevia into 100-120 mesh fine powder and 200 mesh ultrafine powder respectively;

[0038] S2, prepare a three-stage countercurrent extraction tank, Eucommia ulmoides and Apocynum venetum slices and 95% ethanol are put into the first-stage extraction tank according to a ratio of 1:8, and extracted at 60-65°C, 0.3-0.5MPa for 60min. The mixture of Eucommia ulmoides, Apocynum venetum slices and 95% ethanol is separated by a centrifuge and enters the second-stage extraction tank. 70% ethanol is added to the second-stage extraction tank, and the mixture and 70% ethanol are mixed at a ratio of 1:6, and extracted at 55-60°C under normal pressure for 40min. The mixture of Eucommia ulmoides, Apocynum venetum slices and 70% ethanol is separated by a centrifuge and enters the third-stage extraction tank. 50% ethanol is added to the third-stage extraction tank, and the mixture and 50% ethanol are mixed at a ratio of 1:4, and extracted at 45-50°C under normal pressure for 30min. The mixture in the extraction tank is then filtered to obtain an extract.

[0039] S3. Add the crushed kudzu root, hawthorn, cassia seed, corn silk and light bamboo leaf powder into an extraction tank, add 0.1% to 0.3% cellulase and 0.05% to 0.1% pectinase, then adjust the pH to 5.0-6.0, and perform enzymatic hydrolysis at 40-50° C. for 40-80 min. After enzymatic hydrolysis, add 8-15 times the volume of the medicinal materials in water for ultrasonic extraction, extract at an ultrasonic frequency of 40-60 kHz and an ultrasonic power of 300-500 W for 20-40 min, and collect the aqueous extract after filtration;

[0040] S4. The extract and the aqueous extract are combined, and first filtered through a ceramic membrane to remove tannin and protein macromolecular impurities. The pore size of the ceramic membrane is 0.1-0.2 μm, and the operating pressure of the ceramic membrane filtration is 0.3-0.4 MPa. The filtrate is separated by a molecularly imprinted membrane using aucubin as a template molecule to target and retain astringent substances, so that the removal rate of aucubin is ≥90%, while retaining ≥85% of the flavonoid active ingredients, to obtain a refined liquid extracted from the medicinal material;

[0041] S5. The refined liquid, licorice powder and stevia powder are mixed in a ratio of 1:0.5:0.1, transferred to a vacuum concentration tank, concentrated under vacuum conditions, and the concentrate is dried into granules in a drying tower. The granules are then mixed with tea leaves in a ratio of 1:1, and packaged in non-woven tea bags at a ratio of 6 g / bag. The bags are then wrapped with aluminum foil to prevent moisture, thereby obtaining tea bags of Chinese herbal medicine combination tea.

[0042] The relevant experimental data are as follows:

[0043] 1. Animal Experimentation

[0044] 1. Experimental Materials

[0045] 1.1 Experimental Animals

[0046] Sixty SPF male SD rats, 6 weeks old, weighing 200±20 g were used.

[0047] 1.2 Experimental drugs and reagents

[0048] The tea of ​​the present invention, positive control drug: Captopril tablets (50 mg / tablet, produced by Shanghai Xudong Haipu Pharmaceutical Co., Ltd., with approval number H31020617), model inducer: L-NAME (Sigma, purity ≥98%), detection kits: rat ACE activity detection kit, NO / ET-1 ELISA kit, TC / TG / LDL-C / HDL-C detection kit (produced by Nanjing Jiancheng Bioengineering Research Institute).

[0049] 1.3 Experimental instruments

[0050] Non-invasive tail artery blood pressure measurement instrument (CODA non-invasive blood pressure analysis system, manufactured by Shanghai Yuyan Scientific Instrument Co., Ltd., model NIBP-M1), fully automatic biochemical analyzer (manufactured by Beckman Coulter, USA, model AU680).

[0051] 2. Experimental Methods

[0052] 2. Hypertension model building

[0053] After 1 week of adaptive feeding, 60 SD rats were randomly divided into 6 groups, with 10 SD rats in each group:

[0054] SD rats were selected by high-salt diet combined with adrenaline injection. (L-NAME) induced hypertension;

[0055] Normal control group: fed with normal feed and drank distilled water for 28 consecutive days;

[0056] Model control group: fed with normal feed, and L-NAME (50 mg / kg / d) was added to drinking water for 28 consecutive days;

[0057] Positive control group: model mice were gavaged with captopril solution (10 mg / kg / d) for 28 consecutive days;

[0058] Low-dose group of the tea of ​​this application: model mice were gavaged with tea suspension (1g crude drug / kg / d) for 28 consecutive days;

[0059] Medium-dose tea group: Model mice were gavaged with tea suspension (2g crude drug / kg / d) for 28 consecutive days;

[0060] Traditional Eucommia tea group: Model mice were gavaged with commercially available Eucommia tea decoction for 28 consecutive days.

[0061] 2.1. Dosage

[0062] The gavage volume was 10 mL / kg, once a day for 4 consecutive weeks. The model group and the normal group were gavaged with equal volumes of normal saline.

[0063] 3. Experimental index detection

[0064] Blood pressure monitoring:

[0065] Before modeling, 28 days after modeling, and 4 weeks after drug administration, the systolic and diastolic blood pressure of the rats were measured using a tail artery blood pressure meter, and the average value was obtained by measuring 5 times for each SD rat.

[0066] Blood biochemical indicators:

[0067] After the last administration, SD rats were fasted for 12 hours, blood was collected from the abdominal aorta, and serum was separated and tested:

[0068] Blood pressure related: ACE activity, NO / ET-1 content;

[0069] Blood lipid related: TC, TG, LDL-C, HDL-C.

[0070] Histopathological observation:

[0071] Thoracic aorta and kidney tissues of rats were obtained and stained with HE to observe the integrity of vascular endothelium, smooth muscle cell proliferation and glomerular lesions.

[0072] 4. Experimental Data

[0073] Table 1 shows the blood pressure change detection data of SD rats, as follows:

[0074] Group SBP before modeling SBP after modeling SBP after 4 weeks of administration DBP after 4 weeks of administration Normal control group 112±8 115±7 113±6 75±5 Model control group 113±9 165±12* 162±10* 105±8* Positive control group 114±7 163±11* 145±9# 92±7# Low-dose tea group 115±8 164±13* 150±11# 95±6# Medium-dose tea group 116±9 162±12* 138±8## 88±5## Traditional Eucommia Tea Set 113±10 165±14* 155±12# 98±9#

[0075] Table 2 shows the vascular endothelial function and ACE activity indicators of SD rats:

[0076] Group NO (μmol / L) ET-1 (pg / mL) ACE activity (U / mL) Normal control group 85±10 45±6 12.5±1.8 Model control group 52±8* 78±9* 25.3±3.2* Medium-dose tea group 78±9## 55±7## 16.2±2.1## Traditional Eucommia Tea Set 65±7# 68±8# 20.5±2.8#

[0077] Table 3 shows the blood lipid indexes of SD rats:

[0078] Group TC TG LDL-C HDL-C Normal control group 1.8±0.3 0.9±0.2 0.8±0.1 1.2±0.2 Model control group 3.2±0.5* 2.1±0.4* 1.9±0.3* 0.8±0.1* Medium-dose tea group 2.2±0.4## 1.3±0.3## 1.2±0.2## 1.1±0.2## Traditional Eucommia Tea Set 2.8±0.5# 1.8±0.4# 1.6±0.3# 0.9±0.1#

[0079] In the table, * compared with the normal control group, P < 0.01; # compared with the model control group, P < 0.05; ## compared with the medium-dose tea group, P < 0.01;

[0080] Histopathological results

[0081] Aorta: In the model control group, endothelial cell swelling and smooth muscle layer thickening were observed. In the medium-dose tea group, endothelial integrity was significantly improved, and the degree of smooth muscle cell proliferation was lower than that in the traditional Eucommia tea group.

[0082] Kidney: The model control group showed mild hyperplasia of the glomerular mesangial area, the medium-dose tea group showed reduced glomerular lesions, and the degree of tubular interstitial fibrosis was significantly lower than that in the traditional Eucommia tea group.

[0083] 5. Experimental Conclusion

[0084] Multi-target antihypertensive effect:

[0085] After 4 weeks of administration, the medium-dose group of the tea of ​​the present invention showed a 14.8% / 16.2% decrease in systolic and diastolic blood pressure, respectively, compared with the model group. This demonstrated a better antihypertensive effect than the traditional Eucommia tea group and showed no significant difference from captopril (P>0.05). This demonstrated that the tea achieved dual regulation of the RAAS system and vascular endothelial function by inhibiting ACE activity by 36.0%, increasing NO content, and reducing ET-1 levels.

[0086] Improvement of blood lipid metabolism:

[0087] The tea beverage of the present invention can significantly reduce TC (-31.3%), TG (-38.1%), and LDL-C (-36.8%), while increasing HDL-C (+37.5%). The regulatory effect is better than that of traditional Eucommia tea (TC / -12.5%, TG / -14.3%), reflecting the synergistic lipid-lowering effect of medicinal materials such as hawthorn and cassia seed in the formula and the tea ingredients, blocking the pathological association between hypertension and hyperlipidemia.

[0088] Efficacy advantages of process innovation:

[0089] The antihypertensive, lipid-regulating and vascular protection effects of the Chinese herbal tea beverage in this application are superior to those of the eucommia tea prepared by traditional methods. This is attributed to the gradient extraction and membrane refining technology. The dissolution rates of puerarin and hyperoside are improved through enzymatic and ultrasonic synergistic extraction, and the molecular imprinting membrane targets and retains the active ingredients of flavonoids, thereby significantly improving the bioavailability of the medicinal ingredients.

[0090] Security Verification:

[0091] During the experiment, there was no death or obvious behavioral abnormality in any group of rats, and there was no significant difference in liver and kidney function indicators compared with the normal control group, which confirmed that the tea of ​​the present invention is safe for long-term use and provides a reliable basis for long-term conditioning of people with hypertension.

[0092] Example 2

[0093] Specifically: A preparation formula for a combination tea using multiple Chinese medicinal materials, characterized in that the combination tea includes the following raw materials in parts by weight:

[0094] 15-20 parts of Eucommia ulmoides, 12-18 parts of Pueraria root, 10-15 parts of Apocynum venetum, 8-12 parts of Hawthorn, 8-12 parts of Cassia seed, 6-10 parts of Corn silk, 2-4 parts of Licorice root, 0.5-1 part of Stevia rebaudiana, 3-5 parts of Lophatherum gracile and 100 parts of tea.

[0095] The content standards of active ingredients of each medicinal material in the raw materials are: eucommia flavonoids ≥10%, puerarin ≥8%, apocynin ≥1.5%, hyperoside ≥0.8% and bamboo leaf flavonoids ≥3%, and the tea is either black tea or green tea.

[0096] Stevia is a natural sweetener that is 200-300 times sweeter than regular sucrose and contains no calories.

[0097] A method for preparing a combination tea using multiple Chinese medicinal materials, the method comprising the following steps:

[0098] S1, put Eucommia ulmoides and Apocynum venetum into a vibrating screen, vibrate the screen to remove impurities, then cut the sieved Eucommia ulmoides and Apocynum venetum into thin slices, then put Pueraria lobata, Hawthorn, Cassia seed, Corn silk and Bamboo leaf into a crushing device and grind them into 80-100 mesh powder, liquorice and stevia are crushed into 100-120 mesh fine powder and 200 mesh ultrafine powder, respectively;

[0099] S2, prepare three-stage countercurrent extraction tank, eucommia ulmoides and apocynum venetum slices and ethanol are put into the first stage extraction tank according to 1:8, eucommia ulmoides, apocynum venetum slices and ethanol mixture enter the second stage extraction tank after centrifuge separation, ethanol is added to the second stage extraction tank, according to the mixture and ethanol 1:6 mixing, eucommia ulmoides, apocynum venetum slices and ethanol mixture enter the third stage extraction tank after centrifuge separation, then add ethanol to the third stage extraction tank, according to the mixture and ethanol 1:4 mixing, then filter the mixture in the extraction tank to obtain an extract;

[0100] S3, adding the crushed kudzu root, hawthorn, cassia seed, corn silk and light bamboo leaf powder into the extraction tank, adding cellulase and pectinase, after enzymatic hydrolysis, adding 8-15 times the volume of the medicinal materials into water for ultrasonic extraction, and collecting the water extract after filtration;

[0101] S4. The extract and the aqueous extract are combined, first filtered through a ceramic membrane to remove tannin and protein macromolecular impurities, and the filtrate is separated by a molecular imprinting membrane using aucubin as a template molecule to target and intercept astringent substances, so that the removal rate of aucubin is ≥90% while retaining ≥85% of the flavonoid active ingredients, thereby obtaining a refined liquid extracted from the medicinal material;

[0102] S5. The refined liquid, licorice powder and stevia powder are mixed in a ratio of 1:0.5:0.1, transferred to a vacuum concentration tank, concentrated under vacuum conditions, and the concentrate is dried into granules in a drying tower. The granules are then mixed with tea leaves in a ratio of 1:1, and packaged in non-woven tea bags at a ratio of 6 g / bag. The bags are then wrapped with aluminum foil to prevent moisture, thereby obtaining tea bags of Chinese herbal medicine combination tea.

[0103] The three-stage solvent ethanol concentrations of the three-stage countercurrent extraction tank are: 95% ethanol added in the first stage, 70% ethanol added in the second stage, and 50% ethanol added in the third stage;

[0104] The extraction time of each stage of the three-stage countercurrent extraction tank is: the first stage extraction time is 60 minutes, the second stage extraction time is 40 minutes, and the third stage extraction time is 30 minutes.

[0105] During the enzymatic hydrolysis process in S3, the extraction tank is continuously stirred at a stirring speed of 50-80 r / min, and the temperature of the ultrasonically extracted liquid is controlled at a constant temperature of 50-60°C.

[0106] The transmembrane pressure difference of the ceramic membrane filtration in S4 is 0.3-0.4 MPa, the membrane surface flow rate is 3-5 m / s, and the filtration temperature is 20-40°C to avoid degradation of the active ingredients.

[0107] The preparation method of the molecularly imprinted membrane specifically comprises the following steps:

[0108] S4.1. Prepare aucubin, acetonitrile, acrylamide, ethylene glycol dimethacrylate, initiator azobisisobutyronitrile, and polyacrylonitrile-based membrane raw materials, mix aucubin with the acetonitrile solution, then add acrylamide and stir to form a composite solution;

[0109] S4.2, adding ethylene glycol dimethacrylate and initiator azobisisobutyronitrile to the mixture, and then degassing the mixture by ultrasonication for 10 to 20 minutes to remove oxygen to obtain a prepolymer solution;

[0110] S4.3. Immerse the polyacrylonitrile-based membrane in the prepolymerization solution and react in a constant temperature water bath at 60-70°C under nitrogen protection for 12 to 24 hours. After the reaction is completed, take out the membrane and extract it with a methanol / acetic acid mixed solution for 24 to 48 hours to thoroughly elute the template molecules. Finally, rinse the membrane with deionized water until it is neutral and vacuum dry to obtain a molecular imprinting membrane.

[0111] The extract content of tea bags is ≥45%, and the water-soluble components in tea bags account for ≥70%.

[0112] The vacuum concentration tank in S5 maintains normal pressure for 30 to 60 seconds during concentration and then switches to a vacuum degree of 80-100 kPa, and maintains the vacuum state for 30 to 60 seconds. The above switching cycle process is repeated until the refined liquid, licorice powder and stevia powder are concentrated to meet the standards;

[0113] In this embodiment, Eucommia ulmoides and Crataegus pinnatifida: pinoresinol diglucoside in the ethanol extract of Eucommia ulmoides can inhibit renin secretion and block excessive activation of the RAAS system; hyperoside in Crataegus pinnatifida can reduce oxidative stress damage and delay the proliferation of vascular smooth muscle cells by scavenging reactive oxygen species (ROS) in vascular endothelial cells;

[0114] Apocynum venetum leaves and corn silk. The quercetin in Apocynum venetum leaves promotes endothelial relaxation by activating the eNOS / NO pathway, and the flavonoids in corn silk can regulate the expression of water channel proteins, promote sodium ion excretion, and reduce blood volume load.

[0115] Cassia seeds and corn silk can help lower diastolic blood pressure by clearing liver heat, promoting urination, and reducing blood volume load;

[0116] Adding bamboo leaves to traditional Chinese medicine tea combination, the bamboo leaf flavonoids it contains can: inhibit the activity of angiotensin-converting enzyme (ACE), improve microcirculation disorders, and especially have a preventive effect on retinal vascular lesions in patients with hypertension and diabetes;

[0117] The preparation method adopts three-stage dynamic countercurrent extraction technology to process fat-soluble medicinal materials such as Eucommia ulmoides and Apocynum venetum. By setting the ethanol concentration gradient (95%~70%~50%) and regulating temperature and pressure, the layered enrichment of fat-soluble active ingredients such as chlorogenic acid, Eucommia flavonoids, and Apocynum venetum glycosides is achieved. At the same time, enzymatic hydrolysis and ultrasonic synergistic extraction are used for water-soluble medicinal materials such as Pueraria lobata and Crataegus pinnatifida. The plant cell wall structure is destroyed by cellulase and pectinase, and the ultrasonic cavitation effect is combined to accelerate the dissolution of effective ingredients, significantly improving the extraction efficiency of polar components such as puerarin and hyperoside.

[0118] Compared with traditional decoction or simple crushing processes, this process can target the extraction of ingredients based on the characteristics of different medicinal materials, avoid the degradation of heat-sensitive ingredients (such as rutin and bamboo leaf flavonoids) caused by high temperature, and break through the dissolution limitations of medicinal materials with dense cell walls, significantly improving the extraction rate and retention rate of various active ingredients. Subsequent ceramic membrane filtration and molecular imprinting membrane refining further remove large molecular impurities such as tannins and proteins and astringent substances, retaining key medicinal ingredients with a molecular weight of 300-500Da, forming a high-purity, highly active extract. While ensuring the integrity of the effective ingredients, the preparation process greatly improves the utilization rate of medicinal materials, lays a material foundation for the efficacy of the combination tea, and solves the problems of single ingredients, low extraction efficiency, and serious loss of active ingredients in the existing technology, so that the blood pressure-lowering, lipid-regulating, and vascular protection ingredients in the tea can be absorbed and utilized by the human body with higher bioavailability.

[0119] While the embodiments of the present invention have been shown and described, it will be apparent to those skilled in the art that various changes, modifications, substitutions, and alterations can be made to the embodiments without departing from the principles and spirit of the invention.

Claims

1. A preparation formula for a combination tea using multiple Chinese medicinal materials, characterized in that: The combination tea comprises the following raw materials in parts by weight: 15-20 parts of Eucommia ulmoides, 12-18 parts of Pueraria root, 10-15 parts of Apocynum venetum, 8-12 parts of Hawthorn, 8-12 parts of Cassia seed, 6-10 parts of Corn silk, 2-4 parts of Licorice root, 0.5-1 part of Stevia rebaudiana, 3-5 parts of Lophatherum gracile and 100 parts of tea.

2. The preparation formula of a combination tea using multiple Chinese medicinal materials according to claim 1, characterized in that: The content standards of active ingredients of each medicinal material in the raw materials are: eucommia ulmoides flavonoids ≥10%, puerarin ≥8%, apocynin ≥1.5%, hyperoside ≥0.8% and bamboo leaf flavonoids ≥3%, and the tea is one of black tea and green tea.

3. The preparation formula of a combination tea using multiple Chinese medicinal materials according to claim 1, characterized in that: The stevia is a natural sweetener, which is 200-300 times sweeter than ordinary sucrose and contains no calories.

4. A method for preparing a combination tea of ​​multiple Chinese medicinal materials, applied to the preparation formula of a combination tea of ​​multiple Chinese medicinal materials as claimed in any one of claims 1 to 3, characterized in that: The preparation method of the combination tea specifically comprises the following steps: S1, put Eucommia ulmoides and Apocynum venetum into a vibrating screen, vibrate the screen to remove impurities, then cut the sieved Eucommia ulmoides and Apocynum venetum into thin slices, then put Pueraria lobata, Hawthorn, Cassia seed, Corn silk and Bamboo leaf into a crushing device and grind them into 80-100 mesh powder, liquorice and stevia are crushed into 100-120 mesh fine powder and 200 mesh ultrafine powder, respectively; S2, prepare three-stage countercurrent extraction tank, eucommia ulmoides and apocynum venetum slices and ethanol are put into the first stage extraction tank according to 1:8, eucommia ulmoides, apocynum venetum slices and ethanol mixture enter the second stage extraction tank after centrifuge separation, ethanol is added to the second stage extraction tank, according to the mixture and ethanol 1:6 mixing, eucommia ulmoides, apocynum venetum slices and ethanol mixture enter the third stage extraction tank after centrifuge separation, then add ethanol to the third stage extraction tank, according to the mixture and ethanol 1:4 mixing, then filter the mixture in the extraction tank to obtain an extract; S3, adding the crushed kudzu root, hawthorn, cassia seed, corn silk and light bamboo leaf powder into the extraction tank, adding cellulase and pectinase, after enzymatic hydrolysis, adding 8-15 times the volume of the medicinal materials into water for ultrasonic extraction, and collecting the water extract after filtration; S4. The extract and the aqueous extract are combined, first filtered through a ceramic membrane to remove tannin and protein macromolecular impurities, and the filtrate is separated by a molecular imprinting membrane using aucubin as a template molecule to target and intercept astringent substances, so that the removal rate of aucubin is ≥90% while retaining ≥85% of the flavonoid active ingredients, thereby obtaining a refined liquid extracted from the medicinal material; S5. The refined liquid, licorice powder and stevia powder are mixed in a ratio of 1:0.5:0.1, transferred to a vacuum concentration tank, concentrated under vacuum conditions, and the concentrate is dried into granules in a drying tower. The granules are then mixed with tea leaves in a ratio of 1:1, and packaged in non-woven tea bags at a ratio of 6 g / bag. The bags are then wrapped with aluminum foil to prevent moisture, thereby obtaining tea bags of Chinese herbal medicine combination tea.

5. The method for preparing a tea combination using multiple Chinese medicinal materials according to claim 4, characterized in that: The three-stage countercurrent extraction tank has three levels of solvent ethanol concentrations: 95% ethanol is added in the first stage, 70% ethanol is added in the second stage, and 50% ethanol is added in the third stage; The extraction time of each stage of the three-stage countercurrent extraction tank is: the first stage extraction time is 60 minutes, the second stage extraction time is 40 minutes, and the third stage extraction time is 30 minutes.

6. The method for preparing a combination tea using multiple Chinese medicinal materials according to claim 4, characterized in that: During the enzymatic hydrolysis process in S3, the extraction tank is continuously stirred at a stirring speed of 50-80 r / min, and the temperature of the ultrasonically extracted liquid is controlled at a constant temperature of 50-60°C.

7. The method for preparing a tea combination using multiple Chinese medicinal materials according to claim 4, characterized in that: The transmembrane pressure difference of the ceramic membrane filtration in S4 is 0.3-0.4 MPa, the membrane surface flow rate is 3-5 m / s, and the filtration temperature is 20-40° C. to avoid degradation of the active ingredients.

8. The method for preparing a combination tea using multiple Chinese medicinal materials according to claim 4, characterized in that: The method for preparing the molecularly imprinted membrane specifically comprises the following steps: S4.

1. Prepare aucubin, acetonitrile, acrylamide, ethylene glycol dimethacrylate, initiator azobisisobutyronitrile, and polyacrylonitrile-based membrane raw materials, mix aucubin with the acetonitrile solution, then add acrylamide and stir to form a composite solution; S4.2, adding ethylene glycol dimethacrylate and initiator azobisisobutyronitrile to the mixture, and then degassing the mixture by ultrasonication for 10 to 20 minutes to remove oxygen to obtain a prepolymer solution; S4.

3. Immerse the polyacrylonitrile-based membrane in the prepolymerization solution and react in a constant temperature water bath at 60-70°C under nitrogen protection for 12 to 24 hours. After the reaction is completed, take out the membrane and extract it with a methanol / acetic acid mixed solution for 24 to 48 hours to thoroughly elute the template molecules. Finally, rinse the membrane with deionized water until it is neutral and vacuum dry to obtain a molecular imprinting membrane.

9. The method for preparing a tea combination using multiple Chinese medicinal materials according to claim 4, characterized in that: The extract content of the tea bag is ≥45%, and the proportion of water-soluble components in the tea bag is ≥70%.

10. The method for preparing a combination tea using multiple Chinese medicinal materials according to claim 4, characterized in that: The vacuum concentration tank in S5 maintains normal pressure for 30 to 60 seconds during concentration and then switches to a vacuum degree of 80-100 kPa, and maintains the vacuum state for 30 to 60 seconds. The above switching cycle process is repeated until the refined liquid, licorice powder and stevia powder are concentrated to meet the standards.