A method for preparing a night care composition containing multiple plant extract

Abstract

The application discloses a night work activating composition containing multiple plant extract, which comprises the following components in mass: 3-5 parts of extract A, 10-15 parts of extract B, 2-4 parts of extract C, 1.5-3 parts of curcumin nano-liposome, 2-5 parts of lactobacillus fermentation filtrate, 1-3 parts of lock water magnetite, 0.1-0.3 parts of xanthan gum / acrylate compound thickening agent and 0.5-1 part of preservative composition. The night work activating composition containing multiple plant extract of the application is compounded by extract A with methyl jasmonate as a target component, extract B with rhodioside and syringic acid as target components and curcumin nano-liposome, compared with the same kind of products on the market, the antioxidant and anti-glycation capacity of the night work activating composition can be targetedly strengthened, the transdermal efficiency is improved, and the DNA repair effect and anti-inflammatory effect are improved.

Description

Technical Field

[0001] This invention relates to the field of skincare technology, and in particular to a revitalizing composition containing multiple plant extracts for those who stay up late. Background Technology

[0002] In today's fast-paced life, frequent late nights have become the norm for many people. The resulting skin problems, such as dullness, damaged skin barrier, oxidative stress, accumulation of inflammatory factors, and disordered collagen metabolism, have created a huge market demand for "revitalizing skincare after staying up late".

[0003] Chinese patent CN110496090A discloses a composition and its application in the preparation of skincare products for repairing skin damaged by staying up late. The composition provided by this invention includes adenosine, *Sedum aizoon* bulb extract, and sodium hyaluronate. The combination of hyaluronic acid and adenosine achieves skin hydration and moisturization. Small molecule hyaluronic acid can penetrate into the dermis to promote fibroblast proliferation; medium molecule hyaluronic acid can penetrate into the stratum corneum to quickly replenish its moisture; large molecule hyaluronic acid remains on the skin surface to form a film that effectively prevents moisture loss; adenosine, being a small molecule component, can quickly penetrate into the skin, and in addition to being directly converted into energy for cells to use, it can promote cell proliferation. After the skin barrier is repaired, the moisture content is increased, effectively preventing excessive physiological sebum secretion; *Sedum aizoon* bulb extract has a strong cell repair effect, strengthens the skin's natural barrier, slows down the proliferation of melanocytes, inhibits melanin production, and prevents the metabolic waste produced after staying up late from causing inflammation that induces melanin activation, leading to the accumulation of large amounts of melanin in keratinocytes and causing dull skin.

[0004] Chinese patent CN110448478A discloses a composition and its application in the preparation of skincare products that regulate skin circadian rhythms. The composition provided by this invention includes adenosine, a skin conditioning agent A containing glycoproteins and amino acids, and a skin conditioning agent B containing glutamine ethyl imidazole. The active ingredients in skin conditioning agent B can regulate the expression of circadian rhythm genes, promote the repair and proliferation of keratinocytes, promote vitamin D synthesis, and activate vitamin D conversion, thereby activating the skin's barrier function. Skin conditioning agent A, rich in glycoproteins, glutamic acid, valine, and threonine, is used to promote sugar degradation and mitochondrial respiration, ensuring that skin cells maintain a high ATP level throughout the day, ensuring sufficient energy supply when skin cells need repair at night. The additional addition of adenosine directly replenishes the energy needed by cells. This synergistic effect, achieved through circadian rhythm gene regulation, cellular energy stimulation, and additional energy replenishment, addresses skin problems caused by circadian rhythm issues.

[0005] However, in addition to addressing the decreased skin repair ability caused by staying up late, current related products need to specifically address issues such as skin oxidation, inflammation, and collagen metabolism disorders caused by staying up late. Summary of the Invention

[0006] Therefore, it is necessary to provide an overnight revitalizing composition containing multiple plant extracts to address the current situation where existing skincare products are insufficient in improving skin oxidation, inflammation, and collagen metabolism disorders caused by staying up late.

[0007] A method for multiple plant extractions, the invention titled "[Invention Name]", includes the following steps:

[0008] A1. Supercritical fluid extraction of Mirabilis jalapa seeds under the conditions of 25-30 MPa pressure, 40-50℃ temperature, and ethanol (3-5% v / v) as the entrainer. Extraction was performed to obtain extract A, whose target component was methyl jasmonate;

[0009] A2. At a pH of 4.5-5.5, an ultrasonic power of 250-350W, and a treatment time of 20-40min, the roots of Rhodiola rosea and Edelweiss callus were subjected to low-temperature enzymatic hydrolysis-ultrasonic combined extraction with 0.3-0.7% (w / w) cellulase to obtain extract B, whose target components are rhodioloside and Edelweiss acid.

[0010] A3. Green coffee beans were subjected to subcritical water extraction at a temperature of 110-130℃, a pressure of 8-12MPa, and a solvent ratio of water:ethanol = 6:4-8:2 to obtain extract C, whose target component is chlorogenic acid.

[0011] An overnight revitalizing composition containing multiple plant extracts, comprising the aforementioned extracts A, B, and C, specifically comprising, by weight, the following components: 3-5 parts of extract A, 10-15 parts of extract B, 2-4 parts of extract C, 1.5-3 parts of curcumin nanoliposomes, 2-5 parts of Lactobacillus fermentation filtrate, 1-3 parts of water-locking magnet, 0.1-0.3 parts of xanthan gum / acrylate compound thickener, and 0.5-1 parts of preservative composition.

[0012] Preferably, the overnight revitalizing composition comprises, by weight, the following components: 3-4 parts of extract A, 10-12.5 parts of extract B, 3 parts of extract C, 1.5-2.25 parts of curcumin nanoliposomes, 4 parts of lactobacillus fermentation filtrate, 2 parts of water-locking magnet, 0.2 parts of and 0.8 parts of preservative composition.

[0013] Preferably, the overnight revitalizing composition comprises, by weight, the following components: 4-5 parts of extract A, 12.5-15 parts of extract B, 3 parts of extract C, 2.25-3 parts of curcumin nanoliposomes, 4 parts of lactobacillus fermentation filtrate, 2 parts of water-locking magnet, 0.2 parts of and 0.8 parts of preservative composition.

[0014] Preferably, the overnight revitalizing composition comprises, by weight, the following components: 5 parts of extract A, 15 parts of extract B, 3 parts of extract C, 3 parts of curcumin nanoliposomes, 3 parts of Lactobacillus fermentation filtrate, 2 parts of water-locking magnet, 0.2 parts of xanthan gum / acrylate compound thickener, and 0.8 parts of a 3:1 mixture of phenoxyethanol and ethylhexylglycerin.

[0015] Preferably, the overnight revitalizing composition comprises, by weight, the following components: 5 parts of extract A, 12.5 parts of extract B, 2.25 parts of extract C, 3 parts of curcumin nanoliposomes, 3 parts of lactobacillus fermentation filtrate, 2 parts of water-locking magnet, 0.2 parts of xanthan gum / acrylate compound thickener, and 0.8 parts of a 3:1 mixture of phenoxyethanol and ethylhexylglycerin.

[0016] Preferably, the preservative composition is a mixture of phenoxyethanol and ethylhexylglycerin in a mass ratio of 3:1.

[0017] In some embodiments, the amount of extract A incorporated may be set to 3, 3.2, 3.4, 3.6, 3.8, 4, 4.2, 4.4, 4.6, 4.8 or 5.

[0018] In some embodiments, the amount of extract B incorporated may be set to 10, 10.5, 11, 11.5, 12, 12.5, 13, 13.5, 14, 14.5 or 15.

[0019] In some embodiments, the amount of extract C incorporated may be set to 2, 2.2, 2.4, 2.6, 2.8, 3, 3.2, 3.4, 3.6, 3.8 or 4.

[0020] In some embodiments, the amount of curcumin nanoliposomes incorporated can be set to 1.5, 1.7, 1.9, 2.1, 2.3, 2.5, 2.7, 2.9 or 3.

[0021] A method for preparing the above-mentioned overnight revitalizing composition containing multiple plant extracts, the method comprising the following steps:

[0022] B1. Preparation of curcumin nanoliposomes;

[0023] B2. Add 3% w / w trehalose and 2% w / w mannitol to the curcumin nanoliposomes obtained in step B1, pre-freeze at -40℃ and then vacuum dry to obtain nanoliposomes with an activity retention rate of more than 90%.

[0024] B3. Take the predetermined amount of extract B, add 3 parts of β-glucan and dissolve it in sufficient water at 60°C to complete the aqueous phase composition compounding;

[0025] B4. Take a predetermined amount of extract A and the nanoliposomes obtained in step B2, and dissolve 0.5 parts of tocopherol in a sufficient amount of organic solvent at 50°C to complete the oil phase composition compounding.

[0026] B5. The aqueous phase composition from step B3 and the oil phase composition obtained in step B4 are homogenized twice under high pressure at 8000psi to form an O / W emulsion. After cooling, 2 parts of Lactobacillus fermentation filtrate are added to adjust the microecology and obtain the overnight revitalizing composition.

[0027] Preferably, step B1 above includes the following steps:

[0028] B11. Turmeric root powder was ultrasonically degreased three times (30 min each time) with n-hexane (1:10 w / v) to remove grease.

[0029] B12, 70% ethanol, material-to-liquid ratio 1:15, reflux extraction at 50℃ for 2 hours, and concentration to obtain crude curcumin extract;

[0030] B13. Column chromatography purification: silica gel column (200-300 mesh), eluent chloroform:methanol (9:1) to obtain high-purity curcumin;

[0031] B14. Mix phospholipids and cholesterol in a ratio of 7:3 (w / w) to obtain the lipid fraction. Mix curcumin in a ratio of 1:10 (curcumin:total lipids) with the lipid fraction. Then add an appropriate amount of chloroform-methanol (2:1 v / v) to dissolve and vortex mix until transparent.

[0032] B15. Organic solvents were removed by rotary evaporation under reduced pressure at 40°C, resulting in a uniform lipid film in the flask.

[0033] B16. Add ultrapure water containing 0.5% Tween 80 at 46°C to the plasma membrane flask in step B15, and shake in a constant temperature water bath at 46°C for 30 minutes to form multilayer liposomes.

[0034] B17. The above multilayer liposomes are homogenized under high pressure at 15000-18000psi for 3-5 times, with the temperature controlled between 4-10℃ throughout the process, to obtain curcumin nanoliposomes with a particle size of less than 100 nanometers.

[0035] The aforementioned overnight revitalizing composition containing multiple plant extracts, through the combination of extract A (target ingredient: methyl jasmonic acid), extract B (target ingredients: rhodioloside and edelweissic acid), and curcumin nanoliposomes, can specifically enhance the antioxidant and anti-glycation capabilities of the overnight revitalizing composition compared to similar products currently on the market, improve transdermal efficiency, and simultaneously improve DNA repair and anti-inflammatory effects. Specifically:

[0036] (1) In the system of the present invention, based on the antioxidant and penetration-promoting effects of methyl jasmonate and the anti-inflammatory and free radical scavenging effects of curcumin nanoliposomes, methyl jasmonate and curcumin can directly neutralize free radicals while activating the Nrf2 pathway to enhance endogenous antioxidant capacity, thereby forming a dual defense.

[0037] (2) Based on the anti-glycation and mitochondrial protective effects of rhodioloside and the inhibitory effect of chlorogenic acid on lipid peroxidation and microcirculation promotion, rhodioloside and chlorogenic acid can inhibit RAGE and reduce glycation stress, while downregulating LOX / COX to reduce inflammation, thereby amplifying the antioxidant and anti-inflammatory effects of the overnight revitalizing composition.

[0038] (3) Edelweiss acid promotes collagen synthesis through TGF-β, while curcumin inhibits MMP-1 / 9 expression and reduces collagen degradation, thereby achieving a balance between collagen synthesis and degradation. Based on the permeation-enhancing effect of methyl jasmonate, liposomes deliver curcumin to the dermis through the hair follicle bypass, thereby achieving the transdermal-retention synergistic effect of curcumin. Detailed Implementation

[0039] To make the above-mentioned objects, features, and advantages of the present invention more apparent and understandable, specific embodiments of the present invention are described in detail below. Many specific details are set forth in the following description to provide a thorough understanding of the present invention. However, the present invention can be practiced in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0040] This invention discloses a method for multiple plant extractions, which includes the following steps:

[0041] A1. Supercritical fluid extraction of Mirabilis jalapa seeds under the conditions of 25-30 MPa pressure, 40-50℃ temperature, and ethanol (3-5% v / v) as the entrainer. Extraction was performed to obtain extract A, whose target component was methyl jasmonate;

[0042] A2. At a pH of 4.5-5.5, an ultrasonic power of 250-350W, and a treatment time of 20-40min, the roots of Rhodiola rosea and Edelweiss callus were subjected to low-temperature enzymatic hydrolysis-ultrasonic combined extraction with 0.3-0.7% (w / w) cellulase to obtain extract B, whose target components are rhodioloside and Edelweiss acid.

[0043] A3. Green coffee beans were subjected to subcritical water extraction at a temperature of 110-130℃, a pressure of 8-12MPa, and a solvent ratio of water:ethanol = 6:4-8:2 to obtain extract C, whose target component is chlorogenic acid.

[0044] An overnight revitalizing composition containing multiple plant extracts, comprising the aforementioned extracts A, B, and C, specifically comprising, by weight, the following components: 3-5 parts of extract A, 10-15 parts of extract B, 2-4 parts of extract C, 1.5-3 parts of curcumin nanoliposomes, 2-5 parts of Lactobacillus fermentation filtrate, 1-3 parts of water-locking magnet, 0.1-0.3 parts of xanthan gum / acrylate compound thickener, and 0.5-1 parts of preservative composition.

[0045] Preferably, the overnight revitalizing composition comprises the above-mentioned extract A, extract B and extract C. Specifically, the overnight revitalizing composition comprises the following components by weight: 3-4 parts of extract A, 10-12.5 parts of extract B, 3 parts of extract C, 1.5-2.25 parts of curcumin nanoliposomes, 4 parts of lactobacillus fermentation filtrate, 2 parts of water-locking magnet, 0.2 parts and 0.8 parts of preservative composition.

[0046] Preferably, the overnight revitalizing composition comprises the above-mentioned extract A, extract B and extract C. Specifically, the overnight revitalizing composition comprises the following components by weight: 4-5 parts of extract A, 12.5-15 parts of extract B, 3 parts of extract C, 2.25-3 parts of curcumin nanoliposomes, 4 parts of lactobacillus fermentation filtrate, 2 parts of water-locking magnet, 0.2 parts and 0.8 parts of preservative composition.

[0047] Preferably, the preservative composition is a mixture of phenoxyethanol and ethylhexylglycerin in a mass ratio of 3:1.

[0048] In some embodiments, the amount of extract A incorporated may be set to 3, 3.2, 3.4, 3.6, 3.8, 4, 4.2, 4.4, 4.6, 4.8 or 5.

[0049] In some embodiments, the amount of extract B incorporated may be set to 10, 10.5, 11, 11.5, 12, 12.5, 13, 13.5, 14, 14.5 or 15.

[0050] In some embodiments, the amount of extract C incorporated may be set to 2, 2.2, 2.4, 2.6, 2.8, 3, 3.2, 3.4, 3.6, 3.8 or 4.

[0051] In some embodiments, the amount of curcumin nanoliposomes incorporated can be set to 1.5, 1.7, 1.9, 2.1, 2.3, 2.5, 2.7, 2.9 or 3.

[0052] A method for preparing the above-mentioned overnight revitalizing composition containing multiple plant extracts, the method comprising the following steps:

[0053] B1. Preparation of curcumin nanoliposomes;

[0054] B2. Add 3% w / w trehalose and 2% w / w mannitol to the curcumin nanoliposomes obtained in step B1, pre-freeze at -40℃ and then vacuum dry to obtain nanoliposomes with an activity retention rate of more than 90%.

[0055] B3. Take the predetermined amount of extract B, add 3 parts of β-glucan and dissolve it in sufficient water at 60°C to complete the aqueous phase composition compounding;

[0056] B4. Take a predetermined amount of extract A and the nanoliposomes obtained in step B2, and dissolve 0.5 parts of tocopherol in a sufficient amount of organic solvent at 50°C to complete the oil phase composition compounding.

[0057] B5. The aqueous phase composition from step B3 and the oil phase composition obtained in step B4 are homogenized twice under high pressure at 8000psi to form an O / W emulsion. After cooling, 2 parts of Lactobacillus fermentation filtrate are added to adjust the microecology and obtain the overnight revitalizing composition.

[0058] Preferably, step B1 above includes the following steps:

[0059] B11. Turmeric root powder was ultrasonically degreased three times (30 min each time) with n-hexane (1:10 w / v) to remove grease.

[0060] B12, 70% ethanol, material-to-liquid ratio 1:15, reflux extraction at 50℃ for 2 hours, and concentration to obtain crude curcumin extract;

[0061] B13. Column chromatography purification: silica gel column (200-300 mesh), eluent chloroform:methanol (9:1) to obtain high-purity curcumin;

[0062] B14. Mix phospholipids and cholesterol in a ratio of 7:3 (w / w) to obtain the lipid fraction. Mix curcumin in a ratio of 1:10 (curcumin:total lipids) with the lipid fraction. Then add an appropriate amount of chloroform-methanol (2:1 v / v) to dissolve and vortex mix until transparent.

[0063] B15. Organic solvents were removed by rotary evaporation under reduced pressure at 40°C, resulting in a uniform lipid film in the flask.

[0064] B16. Add ultrapure water containing 0.5% Tween 80 at 46°C to the plasma membrane flask in step B15, and shake in a constant temperature water bath at 46°C for 30 minutes to form multilayer liposomes.

[0065] B17. The above multilayer liposomes are homogenized under high pressure at 15000-18000psi for 3-5 times, with the temperature controlled between 4-10℃ throughout the process, to obtain curcumin nanoliposomes with a particle size of less than 100 nanometers.

[0066] In summary, the overnight revitalizing composition of the present invention, containing multiple plant extracts, combines extract A (target component: methyl jasmonate), extract B (target components: rhodioloside and edelweissic acid), and curcumin nanoliposomes. Compared with similar products currently on the market, this composition can specifically enhance the antioxidant and anti-glycation capabilities of the overnight revitalizing composition, improve transdermal efficiency, and simultaneously improve DNA repair and anti-inflammatory effects. Specifically, in the system of the present invention, based on the antioxidant and penetration-promoting effects of methyl jasmonate and the anti-inflammatory and free radical scavenging effects of curcumin nanoliposomes, methyl jasmonate synergistically with curcumin can directly neutralize free radicals while activating the Nrf2 pathway to enhance endogenous antioxidant capacity, thus forming a dual defense. Based on the anti-glycation and mitochondrial protective effects of rhodioloside and the lipid peroxidation and microcirculation rent inhibition effects of chlorogenic acid, the rhodioloside system and chlorogenic acid can inhibit RAGE to reduce glycation stress, while downregulating LOX / COX to reduce inflammation, thereby jointly amplifying the antioxidant and anti-inflammatory effects of the overnight revitalizing composition. In addition, edelweiss acid promotes collagen synthesis through TGF-β, while curcumin inhibits MMP-1 / 9 expression and reduces collagen degradation, thereby achieving a balance between collagen synthesis and degradation. Based on the permeation-enhancing effect of methyl jasmonate, liposomes deliver curcumin to the dermis through the hair follicle bypass, thereby achieving a synergistic effect of transdermal and retention of curcumin.

[0067] The embodiments of the present invention will be described in detail below with reference to examples. It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. For experimental methods in the following embodiments where specific conditions are not specified, please refer to the guidelines given in this invention, or follow experimental manuals or conventional conditions in the art, or follow the conditions recommended by the manufacturer, or refer to experimental methods known in the art.

[0068] Example

[0069] In this embodiment, the overnight revitalizing composition comprises, by weight, the following components: 3 parts of extract A, 10 parts of extract B, 3 parts of extract C, 1.5 parts of curcumin nanoliposomes, 3 parts of Lactobacillus fermentation filtrate, 2 parts of water-locking magnet, 0.2 parts of xanthan gum / acrylate thickener, and 0.8 parts of a 3:1 mixture of phenoxyethanol and ethylhexylglycerin. The overnight revitalizing composition is obtained based on the above-described multiple plant extraction method and the preparation method of the overnight revitalizing composition containing multiple plant extracts.

[0070] Example

[0071] The overnight revitalizing composition was prepared according to the method of Example 1, except that the proportion of extract B was set to 12.5 and the proportion of curcumin nanoliposomes was set to 2.25. Example

[0072] The overnight revitalizing composition was prepared according to the method of Example 1, except that the amount of extract B incorporated was set to 15 and the amount of curcumin nanoliposomes incorporated was set to 3.

[0073] Example

[0074] The overnight revitalizing composition was prepared according to the method of Example 1, except that the amount of extract A incorporated was set to 4; and the amount of curcumin nanoliposomes incorporated was set to 2.25.

[0075] Example

[0076] The overnight revitalizing composition was prepared according to the method of Example 1, except that the amount of extract A incorporated was set to 4; the amount of extract B incorporated was set to 12.5; and the amount of curcumin nanoliposomes incorporated was set to 3.

[0077] Example

[0078] The overnight revitalizing composition was prepared according to the method of Example 1, except that the amount of extract A incorporated was set to 4; and the amount of extract B incorporated was set to 15.

[0079] Example

[0080] The overnight revitalizing composition was prepared according to the method of Example 1, except that the amount of extract A incorporated was set to 5; and the amount of curcumin nanoliposomes incorporated was set to 3.

[0081] Example

[0082] The overnight revitalizing composition was prepared according to the method of Example 1, except that the amount of extract A was set to 5 and the amount of extract B was set to 12.5.

[0083] Example

[0084] The overnight revitalizing composition was prepared according to the method of Example 1, except that the amount of extract A incorporated was set to 5; the amount of extract B incorporated was set to 15; and the amount of curcumin nanoliposomes incorporated was set to 2.25.

[0085] The overnight revitalizing compositions prepared in Examples 1-9 above were tested as follows: ORAC value was tested using the fluorescence decay method; NO inhibition rate was tested using the Griess method; transdermal penetration was tested using the Franz diffusion cell method; and chlorogenic acid retention rate was tested using HPLC. The test indicators included: antioxidant capacity: ORAC value (μmol TE / g); anti-inflammatory effect: NO inhibition rate (%); and transdermal efficiency: chlorogenic acid cumulative penetration over 24 hours (μmol TE / g). Stability: High-temperature retention rate of chlorogenic acid (%). The results are shown in Table 1 below.

[0086]

[0087] A range analysis of the data in the table above yields the following results:

[0088] (1) Curcumin nanoliposomes are the core variable: they have the greatest impact on transdermal efficiency and anti-inflammatory effect.

[0089] (2) Extract A significantly enhances antioxidant capacity: 5 parts added increased the ORAC value by nearly 40%.

[0090] (3) Extract B needs to be added in sufficient quantity: 15 parts are needed for the best anti-glycation and DNA repair effect.

[0091] In summary, the theoretically optimal combination of extract A, extract B, and curcumin nanoliposomes in the overnight revitalizing composition of the present invention is 5 parts extract A, 15 parts extract B, and 3 parts curcumin nanoliposomes. Based on the above orthogonal experiments, its predicted performance includes an ORAC value of 1400-1500 μmol TE / g, a NO inhibition rate of 85-88%, a transdermal penetration rate of 14-15 μg / cm², and a chlorogenic acid retention rate of 90-92%. In actual production, for cost control purposes, the amount of extract B and curcumin nanoliposomes can be adjusted to obtain a balanced combination of 5 parts extract A, 12.5 parts extract B, and 2.25 parts curcumin nanoliposomes, thereby reducing raw material costs by approximately 15%.

[0092] To verify the performance of the above-mentioned optimal doping amount and balanced doping amount compared with currently commercially available products, further comparative verification experiments will be designed below for the theoretical optimal group, the balanced group, and the control group to verify the actual performance of the optimal and balanced schemes of the present invention.

[0093] Comparison Example

[0094] We used commercially available overnight serums containing 3% niacinamide and 1% caffeine to test ORAC, NO inhibition rate, and transdermal efficiency.

[0095] Theoretical Optimal Example

[0096] In this embodiment, the overnight revitalizing composition comprises, by weight, the following components: 5 parts of extract A, 15 parts of extract B, 3 parts of extract C, 3 parts of curcumin nanoliposomes, 3 parts of Lactobacillus fermentation filtrate, 2 parts of water-locking magnet, 0.2 parts of xanthan gum / acrylate thickener, and 0.8 parts of a 3:1 mixture of phenoxyethanol and ethylhexylglycerin. Based on the above-described multiple plant extraction method and the preparation method of the overnight revitalizing composition containing multiple plant extracts, the overnight revitalizing composition was obtained, and ORAC, NO inhibition rate, and transdermal efficiency were tested.

[0097] Balanced Example

[0098] In this embodiment, the overnight revitalizing composition comprises, by weight, the following components: 5 parts of extract A, 12.5 parts of extract B, 3 parts of extract C, 2.25 parts of curcumin nanoliposomes, 3 parts of Lactobacillus fermentation filtrate, 2 parts of water-locking magnet, 0.2 parts of xanthan gum / acrylate thickener, and 0.8 parts of a 3:1 mixture of phenoxyethanol and ethylhexylglycerin. Based on the above-described multiple plant extraction method and the preparation method of the overnight revitalizing composition containing multiple plant extracts, the overnight revitalizing composition was obtained, and ORAC, NO inhibition rate, and transdermal efficiency were tested.

[0099] Subsequently, the verification data of the control example, the theoretically optimal example, and the equilibrium example were compared, and the results are shown in Table 2 below.

[0100]

[0101] In summary, the overnight revitalizing composition of the present invention containing multiple plant extracts has the best overall performance when the component combination is in the theoretical optimal example compared with similar products currently on the market. However, in actual production and sales, it can be adjusted according to the actual production cost. For example, the above-mentioned balanced example has the second best overall performance compared with the theoretical optimal example, but better than the control example.

[0102] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0103] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.

Claims

1. A method for multiple plant extractions, characterized in that, Includes the following steps: A1. Supercritical fluid extraction of Mirabilis jalapa seeds was carried out under the conditions of 25-30 MPa pressure, 40-50℃ temperature, and 3-5% (v / v) ethanol as the entrainer. Extraction was performed to obtain extract A, whose target component was methyl jasmonate; A2. At a pH of 4.5-5.5, an ultrasonic power of 250-350W, and a treatment time of 20-40min, the roots of Rhodiola rosea and Edelweiss callus were subjected to low-temperature enzymatic hydrolysis-ultrasonic combined extraction using cellulase with a mass fraction of 0.3-0.7% to obtain extract B, whose target components are rhodioloside and Edelweiss acid. A3. Green coffee beans were subjected to subcritical water extraction at a temperature of 110-130℃, a pressure of 8-12MPa, and a solvent ratio of water:ethanol = 6:4-8:2 to obtain extract C, whose target component is chlorogenic acid.

2. A revitalizing composition for those who stay up late and contains multiple plant extracts, characterized in that, The overnight revitalizing composition comprises extract A, extract B and extract C as described in claim 1.

3. The overnight revitalizing composition containing multiple plant extracts according to claim 2, characterized in that, The overnight revitalizing composition comprises, by weight, the following components: 3-5 parts of extract A, 10-15 parts of extract B, 2-4 parts of extract C, 1.5-3 parts of curcumin nanoliposomes, 2-5 parts of lactobacillus fermentation filtrate, 1-3 parts of water-locking magnet, 0.1-0.3 parts of xanthan gum / acrylate compound thickener, and 0.5-1 parts of preservative composition.

4. The overnight revitalizing composition containing multiple plant extracts according to claim 2, characterized in that, The overnight revitalizing composition comprises the following components by weight: 3-4 parts of extract A, 10-12.5 parts of extract B, 3 parts of extract C, 1.5-2.25 parts of curcumin nanoliposomes, 4 parts of lactobacillus fermentation filtrate, 2 parts of water-locking magnet, and 0.8 parts of preservative composition.

5. The overnight revitalizing composition containing multiple plant extracts according to claim 2, characterized in that, The overnight revitalizing composition comprises the following components by weight: 4-5 parts of extract A, 12.5-15 parts of extract B, 3 parts of extract C, 2.25-3 parts of curcumin nanoliposomes, 4 parts of lactobacillus fermentation filtrate, 2 parts of water-locking magnet, and 0.8 parts of preservative composition.

6. The overnight revitalizing composition containing multiple plant extracts according to claim 2, characterized in that, The overnight revitalizing composition comprises, by weight, the following components: 5 parts of extract A, 15 parts of extract B, 3 parts of extract C, 3 parts of curcumin nanoliposomes, 3 parts of lactobacillus fermentation filtrate, 2 parts of water-locking magnet, 0.2 parts of xanthan gum / acrylate compound thickener, and 0.8 parts of a 3:1 mixture of phenoxyethanol and ethylhexylglycerin.

7. The overnight revitalizing composition containing multiple plant extracts according to claim 2, characterized in that, The overnight revitalizing composition comprises, by weight, the following components: 5 parts of extract A, 12.5 parts of extract B, 2.25 parts of extract C, 3 parts of curcumin nanoliposomes, 3 parts of lactobacillus fermentation filtrate, 2 parts of water-locking magnet, 0.2 parts of xanthan gum / acrylate compound thickener, and 0.8 parts of a 3:1 mixture of phenoxyethanol and ethylhexylglycerin.

8. The overnight revitalizing composition containing multiple plant extracts according to claim 2, characterized in that, The preservative composition is a mixture of phenoxyethanol and ethylhexylglycerin in a mass ratio of 3:

1.

9. A method for preparing the overnight revitalizing composition containing multiple plant extracts as described in any one of claims 2 to 8, characterized in that, The preparation method of this overnight revitalizing composition includes the following steps: B1. Preparation of curcumin nanoliposomes; B2. Add 3% w / w trehalose and 2% w / w mannitol to the curcumin nanoliposomes obtained in step B1, pre-freeze at -40℃ and then vacuum dry to obtain nanoliposomes with an activity retention rate of more than 90%. B3. Take the predetermined amount of extract B, add 3 parts of β-glucan and dissolve it in sufficient water at 60°C to complete the aqueous phase composition compounding; B4. Take a predetermined amount of extract A and the nanoliposomes obtained in step B2, and dissolve 0.5 parts of tocopherol in a sufficient amount of organic solvent at 50°C to complete the oil phase composition compounding. B5. The aqueous phase composition from step B3 and the oil phase composition obtained in step B4 are homogenized twice under high pressure at 8000psi to form an O / W emulsion. After cooling, 2 parts of Lactobacillus fermentation filtrate are added to adjust the microecology and obtain the overnight revitalizing composition.

10. The method for preparing the overnight revitalizing composition containing multiple plant extracts according to claim 9, characterized in that, Step B1 above includes the following steps: B11. Turmeric root and rhizome powder was ultrasonically degreased three times (30 min / time) with hexane at a material-to-liquid ratio of 1:10 w / v to remove grease. B12, 70% ethanol, material-to-liquid ratio 1:15, reflux extraction at 50℃ for 2 hours, and concentration to obtain crude curcumin extract; B13. Column chromatography purification: silica gel column (200-300 mesh), eluent chloroform:methanol (9:1) to obtain high-purity curcumin; B14. Mix phospholipids and cholesterol in a mass fraction ratio of 7:3 to obtain the lipid fraction. Mix curcumin in a ratio of curcumin to total lipids of 1:10 with the lipid fraction. Then add an appropriate amount of chloroform-methanol in a volume fraction ratio of 2:1 to dissolve and vortex mix until transparent. B15. Organic solvents were removed by rotary evaporation under reduced pressure at 40°C, resulting in a uniform lipid film in the flask. B16. Add ultrapure water containing 0.5% Tween 80 at 46°C to the plasma membrane flask in step B15, and shake in a constant temperature water bath at 46°C for 30 minutes to form multilayer liposomes. B17. The above multilayer liposomes are homogenized under high pressure at 15000-18000psi for 3-5 times, with the temperature controlled between 4-10℃ throughout the process, to obtain curcumin nanoliposomes with a particle size of less than 100 nanometers.

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