Redissolving, diluting and supplementing method for freeze-dried vaccine preparation

Abstract

The invention discloses a freeze-dried vaccine preparation redissolving, diluting and supplementing method which comprises the following steps: orderly arranging and conveying vaccine preparation penicillin bottles through centrifugal force of a centrifugal feeding mechanism, and controlling the number of the penicillin bottles entering an uncovering process by utilizing a flow limiting mechanism; after the bottle opening mechanism is used for automatically opening a cover, an electric liquid extraction needle tube on the mechanical arm is used for extracting a sterile diluent for redissolving the vaccine to dissolve the vaccine freeze-dried powder; and conveying the vaccine reconstitution fluid and the sterile diluent to a vaccine fluid transfer pool according to a preset proportion, uniformly mixing to prepare a required vaccine replenishing fluid, and replenishing the vaccine replenishing fluid to a vaccine soak solution preparation system as required. The device solves the problems of time consumption, labor consumption and accuracy when the redissolving and diluting of a vaccine freeze-drying preparation and the replenishing of an inoculation soaking solution are manually completed in the existing soaking and inoculating process of the fishing vaccine.

Description

Technical Field

[0001] The invention relates to the technical field of aquaculture equipment, and in particular to a method for redissolving, diluting and supplementing a freeze-dried vaccine preparation. Background Art

[0002] During the existing fishery vaccine immersion vaccination process, especially when freeze-dried vaccine preparations are used for immunization, it is necessary to frequently add the diluent after the freeze-dried vaccine preparation is reconstituted to the vaccine immersion vaccination device. However, the existing freeze-dried vaccine reconstitution and diluent addition operations are mostly done manually. For fishery vaccine immersion vaccination machines, the demand for reconstitution and addition of vaccine liquid is large, and the vaccine concentration needs to be adjusted frequently. Manual replenishment is not only time-consuming and labor-intensive, but also difficult to ensure the accuracy and consistency of each replenishment operation. Therefore, there is an urgent need for an automated device with optimized structure and stable operation to solve the above problems. Summary of the Invention

[0003] In order to overcome the defects of the existing technology, a method for reconstitution, dilution and supplementation of freeze-dried vaccine preparations is provided to solve the problems of time-consuming, labor-intensive and inaccurate operations in the existing process of fishery vaccine immersion inoculation by manual reconstitution, dilution and addition of inoculation immersion liquid.

[0004] To achieve the above object, a method for reconstitution, dilution and supplementation of a freeze-dried vaccine preparation is provided, comprising the following steps: A freeze-dried vaccine preparation reconstitution, dilution and supplementation system is provided, which includes a loading mechanism, a first conveyor belt, a positioning mechanism, a bottle opening mechanism, a second conveyor belt, a liquid concentration sensor, an electric liquid withdrawal needle and a controller; The controller turns on the feeding mechanism, and the first motor of the feeding mechanism drives the centrifugal disk to rotate to generate centrifugal force. The vials loaded with freeze-dried vaccine powder on the centrifugal disk are subjected to the centrifugal force and are input to the input end of the first conveyor belt through the discharge port of the limiting cylinder of the feeding mechanism; The controller starts the first conveyor belt to input the vial into the accommodating gap on the upstream side of the working turntable of the positioning mechanism through the output end of the first conveyor belt; The controller turns on the positioning mechanism, and the second motor of the positioning mechanism drives the working turntable to transfer the vial to the midstream side of the working turntable; The controller activates the bottle opening mechanism, which removes the stopper of the vial in the receiving notch on the midstream side of the working turntable; The controller controls the electric liquid extraction needle to extract the diluent from the diluent bottle on the support platform and inject it into the syringe bottle on the positioning mechanism to obtain the vaccine reconstitution solution; The controller controls the electric liquid extraction needle to extract the vaccine reconstitution solution and the diluent, injecting them into the transfer tank and mixing them evenly to obtain the supplementary solution; The controller obtains the concentration value of the vaccine liquid in the vaccine pool collected in real time by the liquid concentration sensor; Based on the concentration value, the controller calculates the dosage and compensation volume of the vaccine reconstitution solution to be replenished; Based on the dosage and compensation volume of the vaccine reconstitution solution, the controller controls the electric liquid extraction needle to extract the supplementary liquid and inject it into the vaccine pool, so that the concentration value of the vaccine solution is within a normal range.

[0005] Furthermore, the controller includes a control module and a calculation module. The calculation module calculates the dosage and compensation volume of the vaccine reconstitution solution to be supplemented based on the concentration value of the vaccine solution. The control module controls the electric liquid extraction needle to extract the diluent from the diluent bottle, inject it into the syringe bottle on the positioning mechanism to obtain the vaccine reconstitution solution, and extract the vaccine reconstitution solution and inject it into the vaccine pool to ensure that the concentration value of the vaccine solution is within the normal range.

[0006] Furthermore, the rotation speed of the centrifugal disk matches the conveying speed of the vial.

[0007] Furthermore, the robotic arm is a multi-degree-of-freedom robotic arm, and the degrees of freedom of the multi-degree-of-freedom robotic arm include horizontal movement freedom and vertical movement freedom.

[0008] Furthermore, when the controller controls the electric liquid extraction needle to extract the vaccine reconstitution solution and diluent into the transfer tank and mix them evenly to obtain the supplementary liquid, the vaccine reconstitution solution and diluent are extracted according to a preset ratio and injected into the transfer tank and mixed evenly to obtain the supplementary liquid.

[0009] Furthermore, the controller dynamically adjusts the amount of the supplementary liquid based on the concentration value of the vaccine liquid in the vaccine pool collected in real time by the liquid concentration sensor.

[0010] The beneficial effect of the present invention is that the method for reconstitution, dilution and supplementation of the freeze-dried vaccine preparation of the present invention realizes the automation of the entire process from automatic feeding of penicillin bottles, smooth transportation, precise opening of caps, efficient liquid extraction to precise dilution, and then to the collection of discarded penicillin bottles and bottle caps, which greatly improves the efficiency and accuracy of vaccine reconstitution, dilution and vaccine liquid replenishment. The method for reconstitution, dilution and supplementation of the freeze-dried vaccine preparation of the present invention not only supports a continuous operating process, but also shows excellent performance in opening penicillin bottles and liquid extraction, ensuring that each step can be completed accurately and without error. The method for reconstitution, dilution and supplementation of the freeze-dried vaccine preparation of the present invention abandons the traditional method of manually opening penicillin bottles and manually extracting vaccine liquid by introducing intelligent automation technology, which saves labor costs and improves work efficiency, and has significant market application potential and good business prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Other features, objects and advantages of the present application will become more apparent upon reading the detailed description of non-limiting embodiments made with reference to the following drawings: Figure 1 Schematic diagram of the process of reconstitution, dilution and supplementation of a freeze-dried vaccine preparation according to an embodiment of the present invention.

[0012] Figure 2 Schematic diagram of the structure of the freeze-dried vaccine preparation reconstitution, dilution and supplementation system according to an embodiment of the present invention.

[0013] Figure 3 Schematic diagram of the structure of the feeding mechanism of an embodiment of the present invention.

[0014] Figure 4 Schematic diagram of the structure of the first conveyor belt according to an embodiment of the present invention.

[0015] Figure 5 Schematic diagram of the positioning mechanism structure of an embodiment of the present invention.

[0016] Figure 6 Schematic diagram of the structure of the bottle opening mechanism according to an embodiment of the present invention.

[0017] Figure 7 Schematic diagram of the structure of the second conveyor belt according to an embodiment of the present invention.

[0018] Figure 8 Schematic diagram of the structure of the robotic arm according to an embodiment of the present invention.

[0019] Figure 9 Schematic diagram of the structure of a first current limiter according to an embodiment of the present invention.

[0020] Figure 10 Schematic diagram of the structure of the working turntable according to an embodiment of the present invention. DETAILED DESCRIPTION

[0021] The present application will be further described in detail below with reference to the accompanying drawings and examples. It should be understood that the specific embodiments described herein are merely for the purpose of explaining the relevant invention and are not intended to limit the invention. It should also be noted that, for ease of description, only portions relevant to the invention are shown in the accompanying drawings.

[0022] It should be noted that, in the absence of conflict, the embodiments and features of the embodiments in this application can be combined with each other. The present application will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0023] The present invention provides a method for reconstitution, dilution and supplementation of a freeze-dried vaccine preparation, which is implemented based on a system for reconstitution, dilution and supplementation of a freeze-dried vaccine preparation.

[0024] Reference Figures 2 to 9 As shown, the freeze-dried vaccine preparation reconstitution, dilution and supplementation system of the present invention includes: a loading mechanism 1, a first conveyor belt 2, a positioning mechanism 3, a bottle opening mechanism 4, a second conveyor belt 5, a support platform 6, a liquid concentration sensor and an electric liquid withdrawal needle 8.

[0025] The loading mechanism includes a limiting cylinder fixed on the workbench, a centrifugal disk for placing the vial and rotatably arranged in the limiting cylinder, and a first motor for driving the centrifugal disk. The cylinder wall of the limiting cylinder is provided with a discharge port arranged toward the top of the centrifugal disk.

[0026] The input end of the first conveyor belt is aligned with the discharge port.

[0027] The positioning mechanism includes a work turntable rotatably mounted on the workbench and a second motor for driving the work turntable. The upstream side of the work turntable is aligned with the output end of the first conveyor belt. The outer edge of the work turntable forms a receiving notch for inserting the vial.

[0028] The bottle opening mechanism is used to remove the stopper of the vial in the receiving gap. The bottle opening mechanism is installed on the workbench and is arranged on the midstream side of the working turntable.

[0029] The input end of the second conveyor belt is aligned with the downstream side of the operation turntable.

[0030] The support platform is used to place the diluent bottle. The support platform is arranged opposite to the second conveyor belt and is installed on the workbench.

[0031] The workbench is equipped with a vaccine pool for containing vaccine liquid. A liquid concentration sensor is installed in the vaccine pool to collect the concentration value of the vaccine liquid.

[0032] The electric liquid extraction needle is installed on the workbench through a robotic arm.

[0033] The controller signal is connected to the feeding mechanism, the first conveyor belt, the positioning mechanism, the bottle opening mechanism, the second conveyor belt, the liquid concentration sensor, the mechanical arm and the electric liquid extraction needle.

[0034] The controller includes a control module and a calculation module.

[0035] The calculation module calculates the molar concentration and compensation volume of the vaccine reconstitution solution to be supplemented based on the concentration value of the vaccine solution.

[0036] Based on the molar concentration and compensation volume of the vaccine reconstitution solution, the control module controls the electric extraction syringe to extract the diluent from the diluent bottle, inject it into the syringe bottle on the positioning mechanism to obtain the vaccine reconstitution solution, and extract the vaccine reconstitution solution and inject it into the vaccine pool to ensure that the concentration value of the vaccine solution is within the normal range.

[0037] In this embodiment, the freeze-dried vaccine preparation reconstitution, dilution and supplementation system of the present invention further includes a workbench 9 .

[0038] Preferably, the workbench is a mobile workbench. It comprises a frame and a tabletop. The tabletop is mounted on the frame. Universal rollers are mounted on the bottom of the frame. The tabletop is used to mount components such as the loading mechanism 1, the first conveyor belt 2, the positioning mechanism 3, the bottle opening mechanism 4, the second conveyor belt 5, the support platform 6, the vaccine reservoir 7, and the electric liquid extraction syringe 8.

[0039] The feeding mechanism 1 includes a limiting cylinder 11 , a centrifugal disc 12 and a first motor 13 .

[0040] Specific, combined Figure 2 and Figure 3 As shown, the limiting cylinder 11 is vertically arranged on the table surface of the workbench 9. The centrifugal disc 12 is rotatably arranged in the limiting cylinder 11. The centrifugal disc and the limiting cylinder are coaxially arranged. The outer diameter of the centrifugal disc is adapted to the inner diameter of the limiting cylinder. The centrifugal disc is used to place the penicillin bottle. The first motor 13 is mounted on the table surface of the workbench and is used to drive the centrifugal disc 12. In this embodiment, the first motor 13 is vertically arranged on the workbench. The output end of the first motor is coaxially connected to the centrifugal disc. A discharge port a is provided on the wall of the limiting cylinder 11. The discharge port a is arranged toward the top of the centrifugal disc 12.

[0041] In this embodiment, the height of the discharge port a is adapted to the height of the vial, the width of the discharge port is slightly larger than the outer diameter of the vial, and the bottom of the discharge port is flush with the upper surface of the centrifugal disk.

[0042] The vials placed on the centrifugal disk slide toward the inner wall of the limiting cylinder under the action of the centrifugal force generated by the first motor driving the centrifugal disk to rotate, and are finally discharged in a single row through the discharge port.

[0043] As a preferred embodiment, the inner wall of the limiting cylinder 11 is paved with a silicone cushion.

[0044] The first motor uses a stepper motor. The limit cylinder is made of ABS engineering plastic, and the inner wall is fitted with a 3mm thick silicone cushion (Shore hardness 30A) to effectively cushion the bottle from collision.

[0045] Combine Figure 4 As shown, the first conveyor belt 2 has an input end and an output end. The input end of the first conveyor belt 2 is aligned with the discharge port a. The discharge port of the limiting cylinder is provided with a guide slope, so that the bottles can smoothly enter the input end of the first conveyor belt at a linear speed of 0.5-1.2m / s.

[0046] In this embodiment, two guide ribs 21 are mounted on the frame of the first conveyor belt 2. The two guide ribs 21 are positioned above the timing belt of the first conveyor belt 2. The guide ribs 21 extend along the length of the timing belt. The distance between the two guide ribs 21 is adapted to the outer diameter of the vials.

[0047] A first flow limiter 91 for limiting the conveying speed of the vials on the first conveyor belt 2 is installed on the workbench 9 .

[0048] Specific, combined Figure 8 As shown, the first flow limiter includes a base 913, a column 912 and a chuck 911. The base is mounted on a workbench. The column is vertically mounted on the base. The chuck is rotatably mounted on the upper end of the column. A plurality of bayonet holes are provided on the outer edge of the chuck. In this embodiment, the number of bayonet holes is four. The size of the bayonet hole is adapted to the outer diameter of the vial. A drive motor for driving the chuck is mounted on the column. The drive motor controls the rotation speed of the chuck. One side of the chuck is arranged above the middle of the synchronous belt of the first conveyor belt. Under the rotation of the chuck, the upper part of the vial on the synchronous belt is embedded in the bayonet hole of the chuck. The conveying speed of the vial on the first conveyor belt is adjusted by the chuck.

[0049] When the bottle density on the second conveyor exceeds the set threshold, the chuck speed is adjusted, and subsequent bottles are retained at the intersection of the centrifugal disc and the input end of the first conveyor until the density returns to normal. The base is fixed to the workbench table with M6 hexagon socket screws, making it easy to disassemble and maintain.

[0050] The flow limiter only allows 4 to 6 vials to enter the capping area (i.e., the working turntable) per cycle. Excess vials are regulated by the PID control algorithm and temporarily stored on the buffer track of the first conveyor belt, which has an overload and blockage warning function.

[0051] The second conveyor belt is a polyurethane synchronous belt with an inner ring equipped with evenly spaced teeth (module 2mm, 120 teeth), which mesh with the drive wheel sprocket. Guide ridges are installed on the upper surface of the synchronous belt to ensure that the vials are transported upright. A photoelectric encoder is installed on the end of the drive wheel shaft to provide real-time speed feedback to the control system.

[0052] The positioning mechanism is arranged at the output end of the first conveyor belt. Figure 4 As shown, the positioning mechanism 3 includes a working turntable 31 and a second motor.

[0053] The work turntable 31 is rotatably mounted on the workbench 9. Specifically, the second motor is fixed to the workbench surface. The work turntable is coaxially connected to the output shaft of the second motor. The second motor drives the work turntable to rotate.

[0054] The outer edge of the work turntable 31 forms a receiving notch b for inserting a vial. In this embodiment, there are multiple receiving notches. The multiple receiving notches are evenly spaced along the circumference of the work turntable. The size of the receiving notches is adapted to the outer diameter of the vial.

[0055] As a preferred embodiment, the inner wall of the accommodating notch b is paved with a rubber anti-skid layer. After the vial is embedded in the accommodating notch, the presence of the rubber anti-skid layer prevents the vial from falling.

[0056] The working turntable is a 600mm diameter aluminum alloy disc with 16 accommodating notches (25mm deep, 2mm chamfered) evenly distributed around its circumference. These notches are inlaid with rubber anti-slip strips. The working turntable is driven by a servo motor (rated power 100W, accuracy ±0.1°) via drive shaft 25, with a speed synchronized with the primary conveyor belt (typically 4 rpm).

[0057] In this embodiment, see Figure 5 and Figure 10 As described above, the working turntable 31 includes an upper disc 311, a lower disc 312 and a connecting shaft 313. The shape and size of the upper disc are adapted to the lower disc. The upper disc and the lower disc are coaxially arranged. The connecting shaft is coaxially connected to the upper disc and the lower disc. Notches are respectively provided on the outer edges of the upper disc and the lower disc. The size of the notch of the upper disc is adapted to the outer diameter of the bottleneck of the vial. The size of the notch of the lower disc is adapted to the outer diameter of the bottle body A of the vial. The size of the notch of the upper disc is smaller than the size of the notch of the lower disc. The notch of the upper disc and the notch of the lower disc constitute an accommodating gap.

[0058] As a preferred embodiment, an arc-shaped anti-slip strip 32 is mounted on the workbench 9. The anti-slip strip 32 is in contact with the circumferential surface of the work turntable. The curvature of the anti-slip strip 32 is adapted to the curvature of the circumferential surface of the work turntable. The anti-slip strip 32 is arranged between the upstream and downstream sides of the work turntable. The distance from the inner curved surface of the anti-slip strip to the inner side of the receiving notch of the work turntable is adapted to the outer diameter of the vial. The restriction and restraint of the anti-slip strip prevents the vial from slipping out of the receiving notch.

[0059] In this embodiment, the work turntable is divided into upstream, midstream, and downstream sides along its rotational direction. The upstream side of the work turntable 31 is aligned with the output end of the first conveyor belt 2. Vials on the first conveyor belt are fed into a receiving notch on the work turntable via the output end. Driven by a second motor, the work turntable rotates, transferring vials on the upstream side of the work turntable to the midstream side.

[0060] The bottle opening mechanism 4 is mounted on the workbench 9. The bottle opening mechanism 4 is arranged on the midstream side of the operation turntable 31. The bottle opening mechanism 4 is used to remove the stopper of the vial in the receiving notch b of the operation turntable 31.

[0061] For details, see Figure 5 As shown, the bottle opening mechanism 4 includes a first bracket 41 and an electric clamping claw 42 .

[0062] The first bracket 41 is mounted on the workbench 9. The electric clamp 42 is mounted on the first bracket 41 in a liftable manner.

[0063] As a preferred embodiment, an upper plate is installed on the top of the first bracket, and a plurality of guide rods are installed on the bottom of the upper plate. A lifting motor is installed on the upper plate. The lifting motor is arranged vertically. The output end of the lifting motor is coaxially connected to a screw rod. A lower plate is slidably provided on the plurality of guide rods. The lower plate is provided with a plurality of vertical through holes. The guide rods are slid in the vertical through holes. The electric clamp is installed on the bottom of the lower plate. Since the lower plate is provided with a threaded hole, the screw rod is screwed into the threaded hole of the lower plate. As the lifting motor drives the screw rod to rotate, the lower plate together with the electric clamp is lifted up and down.

[0064] Continue reading Figure 6 As shown, a diversion ramp 92 is installed on the workbench 9. The upper end of the diversion ramp 92 is arranged below the electric clamp 42. A first collection bucket is installed below the workbench 9. The lower end of the diversion ramp 92 is arranged above the barrel opening of the first collection bucket 93.

[0065] In this embodiment, an elastic baffle is connected to the upper end of the diversion ramp. The elastic baffle is positioned between the electric gripper and the work turntable. When the electric gripper descends and passes over the elastic baffle, it grips the vial stopper on the midstream side of the work turntable. Driven by the lifting motor, it ascends to remove the vial stopper and reverses past the elastic baffle. The electric gripper then releases the stopper, allowing it to fall onto the elastic baffle. Under the influence of its own weight and the diversion ramp, the stopper slides into the first collection bucket.

[0066] Preferably, the inner wall of the guide ramp is sprayed with Teflon coating to reduce friction.

[0067] Combine Figure 2 and Figure 7As shown, the second conveyor belt 5 is positioned between the downstream side of the work turntable and the support platform. The input end of the second conveyor belt 5 is aligned with the downstream side of the work turntable 31. Vials removed from the work turntable are transferred to the downstream side of the work turntable and then fed to the input end of the second conveyor belt. The second conveyor belt then transfers the vials toward its output end.

[0068] The supporting platform 6 is installed on the working platform 9. The vaccine pool 7 is installed on the working platform 9. The supporting platform is arranged on the first side of the second conveyor belt, and the vaccine pool is arranged on the second side of the second conveyor belt.

[0069] The vaccine pool is used to hold the vaccine solution. A liquid concentration sensor is installed in the vaccine pool to collect the concentration of the vaccine solution. In this embodiment, a liquid level meter is installed in the vaccine pool to collect the liquid level of the vaccine solution.

[0070] The supporting platform 6 is used for placing the diluent bottle. The supporting platform 6 is arranged opposite to the second conveyor belt 5. The supporting platform 6 is installed on the workbench through a rotating motor.

[0071] The electric liquid extraction needle tube 8 is installed on the workbench 9 through a mechanical arm. The electric liquid extraction needle tube 8 is used to extract the vaccine in the cillin bottle on the second conveyor belt 5 and the diluent in the dilution bottle and input them into the vaccine pool 7.

[0072] After the electric liquid extraction needle transfers the vaccine in the vial on the second conveyor belt to the vaccine pool, the empty vial is input into the second collection bucket through the output end of the second conveyor belt.

[0073] The robotic arm is a multi-degree-of-freedom robotic arm, and the degrees of freedom of the multi-degree-of-freedom robotic arm include horizontal movement freedom and vertical movement freedom.

[0074] Combine Figure 8 As shown, the robot arm includes a rotary disk 81, a third motor, a second bracket 82, a horizontal linear module 83 and a vertical linear module 84. The electric liquid extraction needle 8 is installed on the slide of the vertical linear module 84.

[0075] The turntable 81 is rotatably mounted on the workbench 9. A third motor is used to drive the turntable 81. The third motor is mounted on the workbench. The second bracket 82 is mounted on the turntable 81. The horizontal linear module 83 is mounted on the top of the second bracket 41. The vertical linear module 84 is mounted on the slide of the horizontal linear module 83. The electric liquid extraction needle 8 is mounted on the slide of the vertical linear module 84. The horizontal linear module 83 is arranged in the horizontal direction, and the vertical linear module 84 is arranged in the vertical direction, allowing the electric liquid extraction needle 8 to move with multiple degrees of freedom in the up and down and left and right directions.

[0076] In this embodiment, the sliders of the horizontal and vertical linear modules are driven by synchronous belts. Specifically, the linear module (i.e., the horizontal or vertical linear module) is equipped with two opposing synchronous pulleys, and the synchronous belt is mounted on the two synchronous pulleys. One side of the synchronous belt is connected to the slide of the linear module. The synchronous pulleys are driven by a sliding motor, which in turn drives the sliders via the synchronous belt.

[0077] As a preferred embodiment, the second conveyor belt utilizes a double-row roller chain drive (12.7mm pitch) and is started and stopped by a stepper motor (with a fine-grained drive accuracy of 1600 pulses / rev). A V-shaped guide track with a depth of 10mm is installed on the surface of the second conveyor belt to ensure stable transport of the bottles to the extraction station after opening. A baffle is installed at the output end of the second conveyor belt. The baffle is height-adjustable (range: 50-100mm), and a corrugated hose is installed on the outside of the baffle. The corrugated hose is connected to the second collection bucket. When baffle 11 triggers a microswitch, the robotic arm pushes the empty bottles into the slide.

[0078] The electric aspiration syringe consists of a base, a syringe, and a drive mechanism. The base is mounted on the slide of a vertical linear module. The drive mechanism is mounted on the base. The drive mechanism drives the piston rod connected to the aspiration syringe via a gear and rack. After the aspiration syringe completes aspiration, a third motor drives the robotic arm to rotate 90°, injecting the vaccine solution into the vaccine reservoir (5L volume, equipped with a liquid level sensor).

[0079] In this embodiment, the workbench comprises a frame. The frame utilizes a 40mm x 40mm aluminum profile frame. The frame's internal interlayer houses pull-out collection buckets (a first collection bucket and a second collection bucket, each with a capacity of 20L). The frame's bottom is equipped with universal casters equipped with brakes and a height-adjustable screw (with an adjustment range of ±10mm). The various electrical components on the workbench are controlled by a PLC integrated module, which coordinates the operation of the motors. Parameters (such as pumping volume and rotational speed) are set on the touchscreen, and abnormal conditions are indicated by audible and visual alarms.

[0080] Continue reading Figure 1 As shown, the method for redissolving, diluting and supplementing the freeze-dried vaccine preparation of the present invention comprises the following steps: S1. The liquid concentration sensor collects the concentration value of the vaccine liquid in the vaccine pool in real time.

[0081] In this embodiment, the liquid concentration sensor uses an embedded concentration sensor to monitor the parameters of the vaccine liquid in real time. The liquid concentration sensor collects the real-time concentration value of the vaccine liquid in the vaccine pool and sends it to the outside.

[0082] S2. The controller obtains the concentration value collected by the liquid concentration sensor.

[0083] The controller includes a control module and a calculation module. The control module acquires the real-time concentration value collected by the liquid concentration sensor. When the concentration of the vaccine solution falls below a preset threshold (X% ± 0.5%), the controller is triggered to initiate a compensation algorithm.

[0084] S3. The controller calculates the dosage and compensation volume of the vaccine reconstitution solution to be supplemented based on the concentration value of the vaccine solution.

[0085] The calculation module calculates the dosage and compensation volume of the vaccine reconstitution solution to be supplemented based on the concentration value of the vaccine solution.

[0086] The controller's calculation module establishes a real-time compensation model, concentration value, and volume of vaccine liquid based on the dynamic mass conservation equation, accurately calculates the required supplementary vaccine liquid dosage (C) and compensation volume (V), and generates a compensation instruction queue.

[0087] S4. The control module turns on the feeding mechanism. The first motor of the feeding mechanism drives the centrifugal disk to rotate to generate centrifugal force. The vials loaded with vaccine freeze-dried powder on the centrifugal disk are subjected to the centrifugal force and input into the input end of the first conveyor belt through the discharge port of the limiting cylinder of the feeding mechanism.

[0088] The rotation speed of the centrifugal disk matches the conveying speed of the vials.

[0089] Vials containing freeze-dried vaccine are placed on a centrifuge disk, which rotates at 250 rpm. Centrifugal force aligns the vials and directs them onto the first conveyor belt. The conveying speed of the first conveyor belt is controlled within the range of 0.5 m / s ± 5%.

[0090] S5. The control module starts the first conveyor belt and inputs the vial into the accommodating gap on the upstream side of the operating turntable of the positioning mechanism through the output end of the first conveyor belt.

[0091] S6. The control module turns on the positioning mechanism, and the second motor of the positioning mechanism drives the operating turntable to transfer the vial to the midstream side of the working turntable.

[0092] S7. The control module activates the bottle opening mechanism, which removes the stopper of the vial from the receiving notch on the midstream side of the working turntable.

[0093] After the vial reaches the midstream side of the bottom working turntable, the stepper motor of the bottle opening mechanism drives the electric gripper to descend to the top of the vial, and the electric gripper of the bottle opening mechanism removes the bottle cap, and the discarded bottle cap slides into the first collection bucket through the diversion ramp for collection.

[0094] S8. The control module controls the electric liquid extraction needle to extract the diluent from the diluent bottle on the support platform based on the dosage and compensation volume of the vaccine reconstitution solution, and injects it into the syringe bottle on the positioning mechanism to obtain the vaccine reconstitution solution.

[0095] The robotic arm on the electric aspiration syringe moves to the diluent bottle according to the preset path, extracts the diluent and injects it into the syringe bottle, and repeatedly aspirates to achieve uniform mixing and redissolution of the vaccine liquid.

[0096] S9. The control module controls the electric liquid extraction needle to extract the vaccine reconstitution solution and the diluent, and injects them into the transfer pool and mixes them evenly to obtain the supplementary solution.

[0097] As a preferred embodiment, the control module controls the electric liquid extraction needle to extract the vaccine reconstituted solution and the diluent according to the preset ratio and inject them into the transfer tank to mix them evenly to obtain the replenishing liquid. S10. Based on the dosage and compensation volume of the vaccine reconstitution solution, the control module of the controller controls the electric liquid extraction needle to extract the supplementary liquid and inject it into the vaccine pool so that the concentration value of the vaccine solution is within a normal range.

[0098] The robotic arm on the electric aspiration syringe moves the aspiration syringe into the syringe bottle according to the preset path, extracts the vaccine reconstitution solution and injects it into the vaccine pool. After mixing, the concentration reaches the normal range.

[0099] In this embodiment, an electric aspiration syringe draws a preset amount of diluent into the vaccine reservoir, where it mixes with the vaccine reconstitution solution to complete the dilution. The diluent dynamically adjusts the amount of vaccine solution added based on feedback from a liquid concentration sensor. The ratio of the injected diluent to the withdrawn vaccine reconstitution solution is a preset value, and the mixed concentration is dynamically adjusted by a controller.

[0100] The method for reconstitution, dilution and supplementation of freeze-dried vaccine preparations of the present invention realizes the automation of the entire process from automatic feeding of vials, smooth transportation, precise opening of caps, efficient liquid extraction, precise dilution, and collection of discarded vials and bottle caps, greatly improving the efficiency and accuracy of vaccine reconstitution, dilution and vaccine liquid supplementation.

[0101] The freeze-dried vaccine preparation reconstitution, dilution and supplementation method of the present invention not only supports a continuous operating process, but also demonstrates excellent efficiency in opening vials and extracting liquids, ensuring that each step can be completed accurately and without error.

[0102] The freeze-dried vaccine preparation reconstitution, dilution and supplementation method of the present invention abandons the traditional method of manually opening the vial and manually extracting the vaccine liquid by introducing intelligent automation technology, which not only saves labor costs but also improves work efficiency. It is suitable for scenarios with strict sterility requirements such as vaccines and biological preparations.

[0103] The freeze-dried vaccine preparation reconstitution, dilution and supplementation system of the present invention is used for the preparation of vaccine soaking liquid for fishery vaccine soaking inoculation in aquaculture. The system arranges and transports vials in an orderly manner through centrifugal force; uses flow limiting to control the number of vials entering the uncapping process; automatically uncaps and extracts diluent to dissolve the freeze-dried vaccine powder; and mixes the reconstituted vaccine liquid with the diluent and adds it to the vaccine pool. The freeze-dried vaccine preparation reconstitution, dilution and supplementation system of the present invention achieves accurate reconstitution, dilution and supplementation of the vaccine liquid through a fully automated process, improves the efficiency of vaccine supplementation, reduces dosage errors, and significantly improves the efficiency and consistency of vaccine liquid preparation. It is suitable for large-scale fishery vaccine soaking inoculation scenarios.

[0104] The single-cycle processing time of the freeze-dried vaccine preparation reconstitution, dilution and supplementation system of the present invention is shortened from 3 minutes in manual operation to 40 seconds, thereby improving the efficiency of vaccine supplementation.

[0105] The liquid extraction error of the freeze-dried vaccine preparation reconstitution, dilution and addition system of the present invention is less than ±0.1 mL, and the dilution concentration deviation is less than ±1%, thereby improving the precision control of vaccine addition.

[0106] The freeze-dried vaccine preparation reconstitution, dilution and supplementation system of the present invention does not require manual intervention throughout the entire process, supports continuous 24-hour operation, and has a high degree of automation.

[0107] The freeze-dried vaccine preparation reconstitution, dilution and supplementation system of the present invention can be integrated into existing fishery vaccine immersion inoculation system equipment, is suitable for large-scale aquaculture farms, significantly reduces manual inoculation costs and improves vaccination success rate and vaccine utilization rate.

[0108] The present invention's freeze-dried vaccine preparation reconstitution, dilution, and replenishment system completely automates the traditional manual process of opening vials to extract vaccine solution, significantly reducing labor costs while improving operational safety and hygiene. This system has broad market application prospects. In the current context of emphasizing automation and intelligent development, this device not only enhances enterprise production capacity and competitiveness but also promotes the development of related industries towards higher quality, possessing extremely high economic value and social benefits.

[0109] The above description is merely a preferred embodiment of the present application and an illustration of the technical principles employed. Those skilled in the art should understand that the scope of the invention herein is not limited to the technical solutions formed by the specific combination of the above-mentioned technical features, but also encompasses other technical solutions formed by any combination of the above-mentioned technical features or their equivalents without departing from the inventive concept. For example, a technical solution formed by replacing the above-mentioned features with (but not limited to) technical features having similar functions disclosed in this application.

Claims

1. A method for redissolving, diluting and supplementing a freeze-dried vaccine preparation, characterized in that: The following steps are involved: A freeze-dried vaccine preparation reconstitution, dilution and supplementation system is provided, which includes a loading mechanism, a first conveyor belt, a positioning mechanism, a bottle opening mechanism, a second conveyor belt, a liquid concentration sensor, an electric liquid withdrawal needle and a controller; The controller turns on the feeding mechanism, and the first motor of the feeding mechanism drives the centrifugal disk to rotate to generate centrifugal force. The vials loaded with freeze-dried vaccine powder on the centrifugal disk are subjected to the centrifugal force and are input to the input end of the first conveyor belt through the discharge port of the limiting cylinder of the feeding mechanism; The controller starts the first conveyor belt to input the vial into the accommodating gap on the upstream side of the working turntable of the positioning mechanism through the output end of the first conveyor belt; The controller turns on the positioning mechanism, and the second motor of the positioning mechanism drives the working turntable to transfer the vial to the midstream side of the working turntable; The controller activates the bottle opening mechanism, which removes the stopper of the vial in the receiving notch on the midstream side of the working turntable; The controller controls the electric liquid extraction needle to extract the diluent from the diluent bottle on the support platform and inject it into the syringe bottle on the positioning mechanism to obtain the vaccine reconstitution solution; The controller controls the electric liquid extraction needle to extract the vaccine reconstitution solution and the diluent, injecting them into the transfer tank and mixing them evenly to obtain the supplementary solution; The controller obtains the concentration value of the vaccine liquid in the vaccine pool collected in real time by the liquid concentration sensor; Based on the concentration value, the controller calculates the dosage and compensation volume of the vaccine reconstitution solution to be replenished; Based on the dosage and compensation volume of the vaccine reconstitution solution, the controller controls the electric liquid extraction needle to extract the supplementary liquid and inject it into the vaccine pool, so that the concentration value of the vaccine solution is within a normal range.

2. The method for reconstitution, dilution and supplementation of a freeze-dried vaccine preparation according to claim 1, characterized in that: The controller includes a control module and a calculation module. The calculation module calculates the dosage and compensation volume of the vaccine reconstitution solution to be supplemented based on the concentration value of the vaccine solution. The control module controls the electric liquid extraction needle to extract the diluent from the diluent bottle, inject it into the syringe bottle on the positioning mechanism to obtain the vaccine reconstitution solution, and extract the vaccine reconstitution solution and inject it into the vaccine pool to ensure that the concentration value of the vaccine solution is within the normal range.

3. The method for reconstitution, dilution and supplementation of a freeze-dried vaccine preparation according to claim 1, characterized in that: The rotation speed of the centrifugal disk matches the conveying speed of the vials.

4. The method for reconstitution, dilution and supplementation of a freeze-dried vaccine preparation according to claim 1, characterized in that: The robotic arm is a multi-degree-of-freedom robotic arm, and the degrees of freedom of the multi-degree-of-freedom robotic arm include horizontal movement freedom and vertical movement freedom.

5. The method for reconstitution, dilution and supplementation of a freeze-dried vaccine preparation according to claim 1, characterized in that: When the controller controls the electric liquid extraction needle to extract the vaccine reconstitution solution and diluent and inject them into the transfer tank for uniform mixing to obtain the supplementary liquid, the vaccine reconstitution solution and diluent are extracted according to a preset ratio and injected into the transfer tank for uniform mixing to obtain the supplementary liquid.

6. The method for reconstitution, dilution and supplementation of a freeze-dried vaccine preparation according to claim 1, characterized in that: The controller dynamically adjusts the amount of the supplementary liquid based on the concentration value of the vaccine liquid in the vaccine pool collected in real time by the liquid concentration sensor.

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