Biological sample detection box

Abstract

The invention belongs to the technical field of biological sample detection, and particularly relates to a biological sample detection box. The invention provides a biological sample detection box, and aims to solve the problems of large size and low detection efficiency of biological sample detection equipment in the prior art. The invention provides a biological sample detection box. Comprising a thin-film micro-fluidic chip integrated in a box body, a preheating assembly used for preheating in a sample extraction process, a driving assembly used for driving liquid to flow in the thin-film micro-fluidic chip, and a temperature control assembly used for heating or cooling the extracted sample in the thin-film micro-fluidic chip. The thin-film micro-fluidic chip is adopted, and the thin-film micro-fluidic chip has a larger contact area with the preheating assembly and the temperature control assembly, so that the heat exchange efficiency is improved, and the sample extraction and detection efficiency is further improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of biological sample detection, and in particular relates to a biological sample detection box. Background Art

[0002] PCR instrument is the abbreviation of PCR amplifier. It is an instrument that uses polymerase chain reaction technology to amplify a specific segment of DNA. It is widely used in medical and biological laboratories.

[0003] The principle of PCR technology is similar to DNA replication, and is composed of three basic reaction steps: denaturation, annealing, and extension. First, the double-stranded DNA is dissociated into single-stranded DNA at high temperature. After cooling, the primers pair with the template DNA single strand. Finally, under the action of polymerase, a DNA chain complementary to the template DNA single strand is synthesized.

[0004] In the prior art, hard chips are usually used as amplification containers for carrying samples. Hard chips are large in size and are heated unevenly, resulting in low amplification efficiency and a large amplification device. Summary of the Invention

[0005] The present invention provides a biological sample detection kit, which aims to solve the problems of large size and low detection efficiency of biological sample detection equipment in the prior art.

[0006] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0007] A biological sample detection box, comprising a thin film microfluidic chip, a temperature control component, a preheating component and a driving component integrated into the box body;

[0008] The temperature control component is used to heat or cool the biological sample after extraction in the thin film microfluidic chip;

[0009] The preheating component is used to preheat the liquid in the thin film microfluidic chip during the extraction of biological samples;

[0010] The driving component is used to drive the liquid in the thin film microfluidic chip to flow in the thin film microfluidic chip according to the biological sample extraction process.

[0011] A further improved solution: the thin film microfluidic chip includes at least two cavities for containing liquid, and at least two cavities are connected through a flow channel. The driving component includes a driving unit for driving the liquid in the thin film microfluidic chip to flow in the thin film microfluidic chip according to the biological sample extraction process and a control unit for controlling the opening and closing of the flow channel.

[0012] Based on the above technical solution: the driving component includes a driving unit and a control unit. The driving unit is used to drive the liquid to flow inside the thin film microfluidic chip according to the extraction work. The control unit is used to control the on and off of the flow channel to prevent the liquid from flowing arbitrarily in the thin film microfluidic chip, thereby improving the sample extraction accuracy of the thin film microfluidic chip.

[0013] A further improved solution: the driving unit includes a first electromagnet, which presses the thin film microfluidic chip at the corresponding cavity through a pressure plate, and the cavity corresponds to the driving unit one by one.

[0014] Based on the above technical solution: the driving unit includes a first electromagnet, which is corresponding to the pressure of the pressure plate. The pressure plate and the thin film microfluidic chip have a larger contact area, so that the liquid in the cavity can be completely squeezed out to prevent residual liquid in the cavity.

[0015] A further improved solution: the control unit includes a second electromagnet, and the second electromagnet squeezes the thin film microfluidic chip at the corresponding flow channel through a pressure head.

[0016] Based on the above technical solution: the control unit includes a second electromagnet, which squeezes the thin film microfluidic chip corresponding to the flow channel through a pressure head. The pressure head and the thin film microfluidic chip have a larger contact area, thereby effectively disconnecting the flow channel and preventing liquid from passing through the flow channel.

[0017] A further improved solution: the control unit further includes a support base for supporting the thin film microfluidic chip, a groove is provided on the support base corresponding to the pressure head, and the pressure head presses a part of the thin film microfluidic chip into the groove.

[0018] Based on the above technical solution: the control unit also includes a support base for supporting the thin film microfluidic chip, and the support base is provided with a groove corresponding to the pressure head. The pressure head can squeeze the part of the thin film microfluidic chip corresponding to the flow channel into the groove, causing the thin film microfluidic chip to bend, thereby better disconnecting the flow channel and preventing the liquid from passing through the flow channel.

[0019] A further improved solution: the cross-sectional shape of the groove is V-shaped; or the cross-sectional shape of the groove is U-shaped.

[0020] Based on the above technical solution: the cross-sectional shape of the groove is V-shaped, or the cross-sectional shape of the groove is U-shaped. There are multiple options for the shape of the groove, which reduces the manufacturing cost of the support seat.

[0021] A further improved solution: the cavity includes a pre-storage chamber, a lysis chamber, a cleaning chamber, an extraction chamber, an elution chamber and an amplification chamber, and the pre-storage chamber, lysis chamber, cleaning chamber, elution chamber and amplification chamber are all connected to the extraction chamber through independent flow channels, and the flow channels correspond one-to-one to the control unit.

[0022] Based on the above technical solution: the chamber includes a pre-storage chamber, a lysis chamber, a cleaning chamber, an extraction chamber, an elution chamber and an amplification chamber. The sample can be extracted and amplified in a thin film microfluidic chip, the sample is not easily contaminated, and the detection accuracy is improved.

[0023] A further improved solution: the preheating component is in contact with the thin film microfluidic chip corresponding to the extraction chamber.

[0024] Based on the above technical solution: the preheating component contacts the thin film microfluidic chip corresponding to the extraction cavity, the preheating component can directly preheat the liquid in the extraction cavity, and the liquid in the extraction cavity is heated evenly.

[0025] A further improved solution: the temperature control component is in contact with the thin film microfluidic chip corresponding to the amplification chamber.

[0026] Based on the above technical solution: the temperature control component is in contact with the thin film microfluidic chip corresponding to the amplification chamber, and the temperature control component directly raises and lowers the temperature of the liquid in the amplification chamber. The liquid in the amplification chamber can quickly complete the temperature rise and fall cycle, thereby improving the amplification efficiency and the detection efficiency.

[0027] A further improved solution: the temperature control component includes a heater in contact with the thin film microfluidic chip and a heat sink arranged on the heater.

[0028] Based on the above technical solution: the temperature control component includes a heater in contact with the thin film microfluidic chip and a radiator arranged on the heater. The temperature control component can quickly switch between heating and cooling modes, thereby improving detection efficiency.

[0029] The beneficial effects of the present invention are:

[0030] The present invention provides a biological sample detection kit comprising a thin-film microfluidic chip integrated within the kit body, a preheating assembly for preheating the sample during extraction, a drive assembly for driving liquid flow within the thin-film microfluidic chip, and a temperature control assembly for heating or cooling the sample after extraction within the thin-film microfluidic chip. The thin-film microfluidic chip provides a larger contact area with the preheating assembly and the temperature control assembly, thereby improving heat exchange efficiency and, in turn, sample extraction and detection efficiency.

[0031] A thin film microfluidic chip is used, and the thin film microfluidic chip is small in size, so that the biological sample detection box has a smaller volume, making the biological sample detection box easy to carry.

[0032] A thin-film microfluidic chip is used, which has a thin wall thickness, improves the heat conduction efficiency, and further improves the sample extraction efficiency and detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following is a brief introduction to the drawings required for use in the embodiments. It should be understood that the following drawings only illustrate certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For users of ordinary skill in the art, other relevant drawings can be obtained based on these drawings without any creative work.

[0034] Figure 1 This is a front view of a biological sample detection kit of the present invention.

[0035] Figure 2 It is a schematic diagram of a biological sample detection kit of the present invention.

[0036] Figure 3 It is a schematic diagram of the arrangement of the driving components in a biological sample detection box of the present invention.

[0037] Figure 4 The figure is a schematic diagram of the arrangement of the driving component relative to the thin film microfluidic chip in a biological sample detection box of the present invention.

[0038] Figure 5 The figure is a front view of a thin film microfluidic chip in a biological sample detection box of the present invention.

[0039] Figure 6 It is a schematic diagram of a control unit in a biological sample detection box of the present invention.

[0040] Description of the numbers in the figure:

[0041] 1-thin film microfluidic chip; 11-pre-storage chamber; 12-lysis chamber; 13-cleaning chamber; 14-extraction chamber; 15-elution chamber; 16-amplification chamber; 17-waste liquid chamber; 2-temperature control component; 21-heater; 22-radiator; 3-drive component; 31-drive unit; 311-pressing plate; 32-control unit; 321-pressing head; 322-support seat; 323-groove. DETAILED DESCRIPTION

[0042] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention. Based on the embodiments of the present invention, all other embodiments obtained by users of this technology in the field without creative work are within the scope of protection of the present invention.

[0043] refer to Figures 1 to 6 , a biological sample detection box, comprising a thin film microfluidic chip 1, a temperature control component 2, a preheating component and a driving component 3 integrated in the box body;

[0044] The temperature control component 2 is used to heat or cool the biological sample after extraction in the thin film microfluidic chip 1;

[0045] The preheating component is used to preheat the liquid in the thin film microfluidic chip 1 during the process of extracting biological samples;

[0046] The driving component 3 is used to drive the liquid in the thin film microfluidic chip 1 to flow in the thin film microfluidic chip 1 according to the biological sample extraction process.

[0047] The preheating component is a structure that can generate heat, and can adopt a semiconductor heating block or other structures.

[0048] The temperature control component 2 is used to output heat or cold to control the temperature increase or decrease of the liquid in the thin film microfluidic control chip to complete the amplification operation.

[0049] The temperature control component 2 can adopt a semiconductor heating element or similar structure.

[0050] refer to Figures 1 to 6 , wherein: the thin film microfluidic chip 1 includes at least two cavities for containing liquid, at least two cavities are connected through a flow channel, and the driving component 3 includes a driving unit 31 for driving the liquid in the thin film microfluidic chip 1 to flow in the thin film microfluidic chip 1 according to the biological sample extraction process and a control unit 32 for controlling the opening and closing of the flow channel.

[0051] The thin film microfluidic chip 1 includes a first thin film layer and a second thin film layer. The first thin film layer and the second thin film layer can be an integrated film structure. The integrated film structure is bent to form the first thin film layer and the second thin film layer. The first thin film layer and the second thin film layer are heat-sealed together, and the unheat-sealed part forms a cavity and a flow channel.

[0052] A heat-sealed area is formed between the first and second film layers, forming the cavity and the flow channel. A heat-sealed portion can be formed within the flow channel to disconnect the flow channel. The heat-sealed portion has a lower seal strength than the heat-sealed area, allowing the seal to be broken open by liquid, thus opening the flow channel. A valve can be used to close the flow channel during sample extraction.

[0053] refer to Figures 1 to 6 , wherein: the driving unit 31 includes a first electromagnet, and the first electromagnet squeezes the thin film microfluidic chip 1 corresponding to the cavity through a pressing plate 311, and the cavity corresponds to the driving unit 31 one by one.

[0054] There are multiple driving units 31 for driving the flow of liquid in different cavities.

[0055] The control unit 32 includes a second electromagnet, which presses the thin film microfluidic chip 1 at the corresponding flow channel through a pressure head 321 .

[0056] The control unit 32 also includes a support base 322 for supporting the thin film microfluidic chip 1. A groove 323 is provided on the support base 322 corresponding to the pressure head 321. The pressure head 321 presses a portion of the thin film microfluidic chip 1 into the groove 323. The control unit 32 has multiple functions for controlling the opening and closing of different flow channels.

[0057] The cross-sectional shape of the groove 323 is V-shaped; or, the cross-sectional shape of the groove 323 is U-shaped.

[0058] refer to Figures 1 to 6 Specifically, the chamber includes a pre-storage chamber 11, a lysis chamber 12, a cleaning chamber 13, an extraction chamber 14, an elution chamber 15 and an amplification chamber 16. The pre-storage chamber 11, the lysis chamber 12, the cleaning chamber 13, the elution chamber 15 and the amplification chamber 16 are all connected to the extraction chamber 14 through independent flow channels, and the flow channels correspond one-to-one to the control unit 32.

[0059] The preheating component is in contact with the thin film microfluidic chip 1 at a position corresponding to the extraction chamber 14. The preheating component is generally used to preheat the liquid in the extraction chamber 14 to 56°C.

[0060] The temperature control component 2 is in contact with the thin film microfluidic chip 1 at a position corresponding to the amplification chamber 16. The temperature control component 2 is used to complete sample amplification, and is generally used to control the sample to cycle between two temperatures.

[0061] The temperature control assembly 2 includes a heater 21 in contact with the thin film microfluidic chip 1 and a heat sink 22 disposed on the heater 21. The heat sink 22 and the heater 21 may be an integrated structure, or the heat sink 22 may be welded to the heater 21.

[0062] The working principle of this embodiment is as follows:

[0063] The thin film microfluidic chip 1 can specifically include a pre-storage chamber 11, a lysis chamber 12, a cleaning chamber 13, an extraction chamber 14, an elution chamber 15 and an amplification chamber 16, and the pre-storage chamber 11, the lysis chamber 12, the cleaning chamber 13, the elution chamber 15 and the amplification chamber 16 are all connected to the extraction chamber 14 through independent flow channels.

[0064] A waste liquid chamber 17 may also be included for storing waste liquid. The waste liquid chamber 17 communicates with the extraction chamber 14 through an independent flow channel. The flow channel connecting the waste liquid chamber 17 and the extraction chamber 14 also corresponds to an independent control unit 32.

[0065] The cleaning chamber 13 may include a second cleaning chamber 13 and a second cleaning chamber 13 . The first cleaning chamber 13 and the second cleaning chamber 13 are arranged in series and communicate with the extraction chamber 14 .

[0066] The biological sample detection kit specifically includes the following detection steps:

[0067] S10, inject the sample into the pre-storage space by any means, then the driving unit 31 corresponding to the pre-storage chamber 11 works, and at the same time, the control unit 32 corresponding to the flow channel connecting the pre-storage chamber 11 and the lysis chamber 12 works to open the flow channel, and the liquid in the pre-storage chamber 11 is squeezed into the lysis chamber 12, and the sample and the reagent are lysed in the lysis chamber 12 at a temperature range of 50°C to 60°C; the sample and the reagent are lysed at a temperature of 50°C, 60°C or 56°C. A guide part can be set in the lysis chamber 12, and the guide part can be formed by heat-sealing the first film layer and the second film layer. The liquid entering the lysis chamber 12 circulates around the guide part for mixing and lysis. During this process, all flow channels communicating with the lysis chamber 12 are closed by the corresponding control unit 32.

[0068] S20, after the liquid in the lysis chamber 12 is completely lysed, the drive unit 31 corresponding to the lysis chamber 12 works, and the control unit 32 for controlling the flow channel between the lysis chamber 12 and the extraction chamber 14 is opened, so that the liquid in the lysis chamber 12 is squeezed into the extraction chamber 14, and the lysed sample is adsorbed in the extraction chamber 14. A silicon cavity membrane for adsorbing the sample can be provided in the extraction chamber 14; in the process of the liquid in the lysis chamber 12 entering the extraction chamber 14, all flow channels connected to the extraction chamber 14 except those connected to the lysis chamber 12 are disconnected, and all flow channels connected to the lysis chamber 12 except those connected to the extraction chamber 14 are disconnected;

[0069] S30, the driving unit 31 corresponding to the first cleaning chamber 13 works, and at the same time, the control unit 32 corresponding to the flow channel connecting the first cleaning chamber 13 and the extraction chamber 14 is opened, and the liquid in the first cleaning chamber 13 is squeezed into the extraction chamber 14, and the sample adsorbed in the extraction chamber 14 is cleaned for the first time using the cleaning liquid in the first cleaning chamber 13. During this process, all the flow channels communicating with the extraction chamber 14 are disconnected except the flow channel communicating with the first cleaning chamber 13, and all the flow channels communicating with the first cleaning chamber 13 are disconnected except the flow channel communicating with the extraction chamber 14. The driving unit 31 corresponding to the extraction chamber 14 can work repeatedly to squeeze the liquid in the extraction chamber 14 so that the sample in the extraction chamber 14 is repeatedly cleaned. During the cleaning process, the pressing plate 311 corresponding to the extraction chamber 14 can work repeatedly within 1 / 2 stroke, or less than 2 / 3 stroke, to repeatedly squeeze the extraction chamber 14 to complete the first cleaning of the sample. After the sample in the extraction chamber 14 completes the first cleaning, the driving unit 31 corresponding to the extraction chamber 14 works, and the channel connecting the extraction chamber 14 to the waste liquid chamber 17 is opened, and the waste liquid enters the waste liquid chamber 17. Alternatively, the driving unit 31 corresponding to the extraction chamber 14 works, and the channel connecting the lysis chamber 12 is opened, and the waste liquid enters the lysis chamber 12.

[0070] S40, referring to the cleaning process of the first cleaning chamber 13, the sample in the extraction chamber 14 that has completed the first cleaning is cleaned for a second time using the cleaning liquid in the second cleaning chamber 13, wherein the cleaning process preheating component works, and the sample after the first cleaning is cleaned for a second time in the temperature range of 60° C. to 70° C., preferably, the second cleaning is performed at 65° C. The liquid in the second cleaning chamber 13 can enter the extraction chamber 14 through the first cleaning chamber 13, or the second cleaning chamber 13 can also be an independent chamber. The second cleaning process of the sample in the extraction chamber 14 by the second cleaning chamber 13 is similar to the first cleaning process, and the waste liquid can still enter the waste liquid chamber 17 or the lysis chamber 12;

[0071] At step S50, after the second cleaning of the liquid in the extraction chamber 14 is completed, the drive unit 31 corresponding to the elution chamber 15 operates to squeeze the liquid in the elution chamber 15 into the extraction chamber 14. The sample after the second cleaning is eluted using the eluent in the elution chamber 15 at a temperature range of 60°C to 70°C, thereby completing the extraction of the biological sample. Preferably, the elution is performed at 65°C. During this process, all flow channels communicating with the extraction chamber 14, except those communicating with the elution chamber 15, are disconnected.

[0072] At step S60, after the sample is extracted from the extraction chamber 14, the drive unit 31 corresponding to the extraction chamber 14 is activated, disconnecting all flow channels connecting the extraction chamber 14 except the one connecting to the amplification chamber 16, and squeezing the sample from the extraction chamber 14 into the amplification chamber 16. The flow channels connecting to the amplification chamber 16 are then closed by the corresponding control unit 32, and the temperature control assembly 2 is activated to amplify the sample in the amplification chamber 16.

[0073] Finally, the biological sample detection kit may further include an optical system for optically detecting the sample after amplification.

[0074] The present invention is not limited to the above-mentioned optional implementation methods. Under the premise of not conflicting with each other, the various solutions can be arbitrarily combined. Anyone can derive other forms of products under the inspiration of the present invention. However, no matter what changes are made in their shape or structure, all technical solutions that fall within the scope defined by the claims of the present invention fall within the scope of protection of the present invention.

Claims

1. A biological sample detection kit, characterized in that: It includes a thin film microfluidic chip, a temperature control component, a preheating component and a driving component integrated in the box body; The temperature control component is used to heat or cool the biological sample after extraction in the thin film microfluidic chip; The preheating component is used to preheat the liquid in the thin film microfluidic chip during the extraction of biological samples; The driving component is used to drive the liquid in the thin film microfluidic chip to flow in the thin film microfluidic chip according to the biological sample extraction process.

2. The biological sample detection kit according to claim 1, characterized in that: The thin film microfluidic chip includes at least two cavities for containing liquid, and at least two cavities are connected through a flow channel. The driving component includes a driving unit that drives the liquid in the thin film microfluidic chip to flow in the thin film microfluidic chip according to the biological sample extraction process and a control unit that controls the opening and closing of the flow channel.

3. The biological sample detection kit according to claim 2, characterized in that: The driving unit includes a first electromagnet, which presses the thin film microfluidic chip at the corresponding cavity through a pressing plate, and the cavity corresponds to the driving unit one by one.

4. The biological sample detection kit according to claim 2, characterized in that: The control unit includes a second electromagnet, and the second electromagnet presses the thin film microfluidic chip at the corresponding flow channel through a pressure head.

5. The biological sample detection kit according to claim 1, characterized in that: The control unit further comprises a support base for supporting the thin film microfluidic chip. A groove is provided on the support base corresponding to the pressure head, and the pressure head presses a part of the thin film microfluidic chip into the groove.

6. The biological sample detection kit according to claim 5, characterized in that: The cross-sectional shape of the groove is V-shaped; or, the cross-sectional shape of the groove is U-shaped.

7. The biological sample detection kit according to claim 2, characterized in that: The cavity includes a pre-storage chamber, a lysis chamber, a cleaning chamber, an extraction chamber, an elution chamber and an amplification chamber. The pre-storage chamber, the lysis chamber, the cleaning chamber, the elution chamber and the amplification chamber are all connected to the extraction chamber through independent flow channels, and the flow channels correspond one to one with the control unit.

8. The biological sample detection kit according to claim 7, characterized in that: The preheating component contacts the thin film microfluidic chip corresponding to the extraction cavity.

9. The biological sample detection kit according to claim 7, characterized in that: The temperature control component contacts the thin film microfluidic chip corresponding to the amplification chamber.

10. The biological sample detection kit according to claim 9, characterized in that: The temperature control component includes a heater in contact with the thin film microfluidic chip and a heat sink arranged on the heater.

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