Evaporation and concentration mechanism and automatic analysis device with evaporation and concentration mechanism

Abstract

The invention provides an evaporation and concentration mechanism capable of adjusting the liquid evaporation amount through a low-cost and simple mechanism and an automatic analysis device provided with the mechanism. The intake part (310) includes an exhaust flow path (314), one end of which is connected to the exhaust part (320) and the other end of which is connected to an opening of the reaction vessel by the intake part contacting the reaction vessel; and a through-hole (312) through which external air can flow into the reaction vessel, the exhaust unit includes a negative pressure generation source (321) for generating negative pressure, an exhaust pipe (324) connecting the negative pressure generation source and an exhaust flow path of the intake unit, and an atmosphere introduction unit (322) for introducing atmosphere into the exhaust pipe, and an atmosphere introduction pipe (323) in the atmosphere introduction unit is detachable.

Description

Technical Field

[0001] The invention relates to an evaporation and concentration mechanism and an automatic analysis device having the evaporation and concentration mechanism. Background Art

[0002] As an analysis method for analyzing a specific component contained in a sample, for example, LC-MS in which a liquid chromatograph (LC: Liquid Chromatography) and a mass spectrometer (MS: Mass Spectrometry) are connected online can be used.

[0003] The use of LC-MS is also expanding into the field of clinical testing, where biological samples such as blood and urine are analyzed using automated analyzers. When analyzing biological samples such as blood or urine (hereinafter referred to as samples) using LC-MS, pretreatment is required to improve the purity of the sample. For example, the target components contained in the sample are extracted using methods such as solid phase extraction (SPE) or liquid-liquid extraction (LLE). In addition, to achieve high-sensitivity detection using LC-MS, evaporation concentration is sometimes performed, which increases the concentration of the target components by evaporating the extract containing the target components.

[0004] As such a technique for evaporating and concentrating a sample, there are known techniques such as those described in Patent Documents 1 and 2. In the evaporation process described in Patent Document 1, a reaction vessel for holding a sample liquid is connected to a reduced pressure source via a reduced pressure flow path, and a concentration process is performed by reducing the pressure inside the reaction vessel. In addition, Patent Document 2 discloses a plug for removing volatile substances, which is suitable for vaporizing and removing volatile substances from a solution containing volatile substances contained in a container. A through hole for discharging the vaporized volatile substances is formed in the plug for removing volatile substances, and a groove is formed on its side surface, connecting a gas inlet located at the upper edge of the plug and a gas outlet located at the lower edge of the plug. By reducing the pressure of the container, the volatile substances are stirred by the gas blown into the container at high speed through the groove, thereby promoting the vaporization of the volatile substances. Prior art literature Patent Literature

[0005] Patent Document 1: International Publication No. 2022 / 185707 Patent Document 2: International Publication No. 2008 / 078765 Summary of the Invention Technical problem to be solved by the invention

[0006] Automatic analyzers are used in a variety of environments. For example, when used at high altitudes, the lower the atmospheric pressure compared to flat land, the lower the boiling point and the more readily evaporate, potentially exceeding the designed evaporation rate. While Patent Document 1 discloses controlling the decompression rate during the concentration process, the final decompression rate remains the same regardless of the decompression rate, and no consideration is given to regulating the evaporation rate. Patent Document 2 also fails to consider regulating the vaporization rate of volatile substances.

[0007] An object of the present invention is to provide an evaporation concentration mechanism capable of adjusting the evaporation amount of a liquid by an inexpensive and simple mechanism, and an automatic analyzer having the evaporation concentration mechanism. Technical means for solving technical problems

[0008] An evaporation and concentration mechanism as an embodiment of the present invention evaporates and concentrates a liquid contained in a reaction container, and includes: a container setting portion for setting the reaction container; an air inlet portion, which can be in contact with the reaction container in a manner covering the opening of the reaction container; and an exhaust portion, which exhausts the gas in the reaction container via the air inlet portion, the air inlet portion including an exhaust flow path, one end of the exhaust flow path being connected to the exhaust portion, and the other end being connected to the opening of the reaction container through contact with the reaction container by the air inlet portion; and a through hole, which can allow external air to flow into the reaction container, the exhaust portion including a negative pressure generating source for generating negative pressure, an exhaust pipe connecting the negative pressure generating source and the exhaust flow path of the air inlet portion, and an atmosphere introduction portion for introducing atmosphere into the exhaust pipe, the atmosphere introduction pipe in the atmosphere introduction portion being detachable. Effects of the Invention

[0009] The present invention provides an evaporation concentration mechanism that can adjust the evaporation amount by a cheap and simple mechanism and an automatic analyzer equipped with the evaporation concentration mechanism. Other problems, structures and effects other than the above will become more apparent through the following description of the embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 It is a diagram schematically showing the overall structure of an automatic analyzer. Figure 2 This is a diagram showing an example of a pre-processing step of an analysis process by an automatic analyzer. Figure 3A This is a diagram schematically showing an example of an evaporation concentration mechanism. Figure 3B This is a diagram schematically showing an example of an evaporation concentration mechanism. Figure 4 This is a graph showing the difference in evaporation rate depending on whether or not an atmospheric air introduction pipe is connected. Figure 5This is a graph showing the difference in evaporation rate depending on the length of the atmosphere introduction pipe. Figure 6 This is a diagram showing the relationship between the atmospheric air introduction pipe and the amount of external air flowing in. Figure 7 It is a diagram schematically showing a state in which steam is sucked into the air intake portion. DETAILED DESCRIPTION

[0011] The following describes an embodiment of the present invention with reference to the accompanying drawings. While this embodiment exemplifies an automated analyzer that combines an LC-MS analyzer with a sample pretreatment mechanism, the present invention can also be applied to automated analyzers that combine a separation mechanism such as capillary electrophoresis with a detector such as an absorptiometry as an analyzer.

[0012] Figure 1 This figure schematically illustrates the overall structure of an automatic analyzer. The main components of the automatic analyzer 100 include a pretreatment unit 101 for pre-treating a sample, a separation unit 102 for separating components in the sample, an analysis unit 103 for analyzing the separated components, and a control unit 104 for controlling the various mechanisms within the device and analyzing and outputting the detection results of the analysis unit 103.

[0013] The pretreatment section 101 includes a conveying mechanism 112 for conveying a sample container 111 containing a sample to be analyzed to a sample dispensing position; an incubator (reaction container disk) 120 for maintaining a constant temperature of the solution within the reaction container 116 by mounting the reaction container 116 on a plurality of openings 119; a reagent disk 122 for holding a plurality of reagent containers 121 containing reagents; and a sample dispensing mechanism 113 for dispensing the sample from the sample container 111 conveyed to the sample dispensing position into the reaction container 116 contained in the opening 119 of the incubator 120. To avoid contamination, the sample dispensing mechanism 113 installs a dispensing needle when dispensing the sample. The dispensing needle is attached and detached in the dispensing needle attachment and detachment section 114. Unused dispensing needles 115a stored in the dispensing needle carrier 115 are conveyed to the dispensing needle attachment and detachment section 114 by the conveying mechanism 118.

[0014] In addition, the pretreatment unit 101 includes a magnetic separation mechanism 124, which separates magnetic beads in the solution contained in the reaction container 116 through the magnetic force of a magnet; a conveying mechanism 125, which conveys the reaction container 116 between the incubator 120 and the magnetic separation mechanism 124; and an evaporation and concentration mechanism 131, which evaporates and concentrates the analysis object components in the solution in the reaction container 116.

[0015] In addition, the pretreatment section 101 includes a conveying mechanism 132, which conveys the reaction container 116 between the incubator 120 and the evaporation and concentration mechanism 131; and a separation section dispensing mechanism 133, which dispenses the solution in the reaction container 116 after evaporation and concentration into the separation section 102 for separating the components in the sample.

[0016] The magnetic separation mechanism 124 is provided on the rotation track 126 of the reagent dispensing mechanism 123. The reagent dispensing mechanism 123 can discharge the reagent into the reaction container 116 supported by the magnetic separation mechanism 124 or can aspirate the solution in the reaction container 116.

[0017] The incubator 120 has a function of maintaining a constant temperature of the reaction container 116 placed in the opening 119 , and incubates the reaction container 116 placed in the opening 119 for a predetermined period of time.

[0018] The separation unit 102 is, for example, an LC system and includes a column or the like for separating the components of the reaction solution dispensed by the separation unit dispensing mechanism 133. The separation unit 102 separates the components of the reaction solution dispensed from the reaction vessel 116 using the separation unit dispensing mechanism 133 and sequentially introduces the separated components to the analysis unit 103. The analysis unit 103 is, for example, an MS, and ionizes the components introduced from the separation unit 102, detects the ion amounts (i.e., the component amounts), and outputs the detection results to the control unit 104.

[0019] First, the basic steps of the analysis process will be described. Figure 2 1 is a diagram illustrating an example of a pre-processing step of an analysis process in the automatic analyzer 100. Before starting the pre-processing, unused reaction vessels 116 are placed in advance in an opening 119 of an incubator 120 from a reaction vessel rack 117 by a conveyor mechanism 118.

[0020] First, a sample containing an analyte component is sucked from the sample container 111 by the sample dispensing mechanism 113 and discharged into the reaction container 116 in the incubator 120 (step S200 ).

[0021] Next, the reagent dispensing mechanism 123 draws an internal standard substance from the reagent container 121 on the reagent disk 122 as a reagent corresponding to the analyte component and discharges it into the reaction container 116 (step S201). Next, the reagent dispensing mechanism 123 draws a reagent, such as a deproteinizing agent, from the reagent container 121 on the reagent disk 122 and discharges it into the reaction container 116 (step S202). Next, the reagent dispensing mechanism 123 draws a suspension of magnetic beads from the reagent container 121 on the reagent disk 122 as a reagent and discharges it into the reaction container 116 (step S203).

[0022] Thereafter, the reaction container 116 into which the sample, internal standard substance, reagent, and magnetic beads are dispensed is transported to the magnetic separation mechanism 124 by the transport mechanism 125 , and the magnetic beads 207 are washed (step S204 ).

[0023] The cleaning process of the magnetic beads is described below. In the magnetic separation mechanism 124, the magnetic force of the magnet 201 arranged along the outer side of the reaction vessel 116 is used to collect the magnetic beads holding the analyte components and the internal standard substance onto the inner wall surface ( Figure 2 16). In this state, the solution of the reaction vessel 116 is sucked in and discarded by the reagent dispensing mechanism 123. At this time, the magnetic beads, the analysis target components and the internal standard substances retained in the magnetic beads remain in the reaction vessel 116. Next, a cleaning solution for cleaning impurities other than the substances (analysis target components, internal standard substances) retained in the magnetic beads is sucked in from the reagent container 121 of the reagent disk 122 by the reagent dispensing mechanism 123, and the cleaning solution is discharged into the reaction vessel 116. At this time, the magnetic force of the magnet 201 can be temporarily released from the constraint of the magnetic beads. Thereafter, in a state where the magnetic beads are collected on the inner wall surface of the reaction vessel 116 by the magnet 201, the reagent dispensing mechanism 123 sucks in and discards the solution (cleaning solution) in the reaction vessel 116, thereby cleaning the magnetic beads.

[0024] Next, the reagent dispensing mechanism 123 sucks the eluent used to dissolve and separate the analyte components and the internal standard substance from the magnetic bead group 202 as a reagent from the reagent container 121 of the reagent disk 122 and discharges it into the reaction container 116 (step S205).

[0025] Next, while the magnetic bead group 203, in which the analyte component and the internal standard substance have been dissolved and separated, is collected on the inner wall surface of the reaction vessel 116 by the magnetic force of the magnet 201, the solution (purified liquid) in the reaction vessel 116 is aspirated by the reagent dispensing mechanism 123 (step S206) and discharged into an unused reaction vessel 116v in the incubator 120, which is different from the reaction vessel 116 disposed in the magnetic separation mechanism 124 (step S207). Furthermore, the purified liquid contained in the reaction vessel 116v is incubated as needed.

[0026] Next, the reaction container 116v containing the purified liquid is transported to the evaporation and concentration mechanism 131 by the transport mechanism 132, and the components in the purified liquid are evaporated and concentrated (step S208). The detailed structure of the evaporation and concentration mechanism 131 will be described later.

[0027] In addition, when the composition of the purified liquid is changed so that the analyte component is combined with the separation column (not shown) included in the separation unit 102, the diluent is sucked from the reagent container 121 of the reagent disk 122 by the reagent dispensing mechanism 123 and discharged into the reaction container 116v.

[0028] The purified liquid obtained through the above pretreatment step is drawn from the reaction vessel 116 and discharged into the separation unit 102 by the separation unit dispensing mechanism 133. The components separated in the separation unit 102 are ionized in the analysis unit 103, and the ion amounts (i.e., component amounts) are detected. The detection results obtained by the analysis unit 103 are output to the control unit 104, which calculates the concentration values ​​of the components in the sample using a calibration curve.

[0029] The evaporation concentration process in this embodiment will be described. The automatic analyzer 100 can adjust the evaporation amount to meet the evaporation performance required by the device.

[0030] Figure 3A and Figure 3B 1 is a diagram schematically showing an example of the evaporation and concentration mechanism 131. The evaporation and concentration mechanism 131 includes a container installation portion 302 for installing a reaction container 116v containing the purified liquid obtained in the pretreatment step, an air intake portion 310 for drawing vapor (gas) from the reaction container 116v, and an air exhaust portion 320. Figure 3A The container installation part 302 and the air intake part 310 are shown in a bird's-eye view. The container installation part 302 includes one or more container storage parts 301 for storing the reaction container 116v and a heating part 303 for heating the reaction container 116v. A Peltier element or a heater capable of controlling the temperature can be used as the heating part 303. In addition, the air intake part 310 includes an arm 311 and a support 313. An exhaust flow path 314 (see FIG. 314 ) for sucking in the steam in the reaction container 116v is provided. Figure 7 ) is formed within arm 311 and support 313. Exhaust flow path 314 is formed such that one end is connected to exhaust portion 320, and the other end is connected to the opening of reaction vessel 116v by contacting arm 311 so as to cover reaction vessel 116v. Furthermore, arm 311 is provided with a through-hole 312 for allowing outside air to flow into reaction vessel 116v when steam is inhaled.

[0031] Figure 3BThe structure of the exhaust unit 320 is shown. The exhaust unit 320 includes an exhaust mechanism 321 and an atmosphere introduction portion 322 for introducing outside air midway between the exhaust flow path 314 in the intake unit 310 and the exhaust pipe 324 of the exhaust mechanism 321. An atmosphere introduction pipe 323 is detachably attached to the atmosphere introduction portion 322. Whether the atmosphere introduction pipe 323 is connected to the atmosphere introduction portion 322 is optional. By not connecting the atmosphere introduction pipe 323 and sealing the atmosphere introduction pipe connection portion of the atmosphere introduction portion 322, the introduction of outside air can be prevented. In addition, Figure 3B In the example, an atmosphere introduction portion is included, and the front end of the atmosphere introduction pipe 323 is open to the atmosphere, but it is not limited to this. For example, multiple atmosphere introduction portions 322 can be provided, or an opening portion can be provided in the middle of the atmosphere introduction pipe 323 so as to be open to the atmosphere, or multiple opening portions can be provided in the atmosphere introduction pipe 323. The atmosphere introduction portion 322 can be configured as a tube having a branch portion such as a Y-shaped tube or a T-shaped tube, or can be configured as a block having a hole for gas flow inside. The hole provided in the block only needs to allow the gas to circulate, and does not need to be tubular. A shape that is easy to form, such as a triangular or quadrilateral cross-section, can be appropriately selected. Two blocks provided with grooves can be docked and joined. As an example of the exhaust mechanism 321, a negative pressure generating source that exhausts by generating negative pressure can be cited, such as a vacuum pump such as a rotary pump. Since a high vacuum degree is not required, any so-called roughing pump can be used.

[0032] Furthermore, when steam is drawn in through the air inlet 310, the condensed steam may liquefy in the air inlet 310 and drip. When these drips enter the reaction vessel, the evaporation concentration rate decreases, so it is desirable to take measures to prevent dripping. For example, heating the air inlet 310 to prevent steam from condensing on its surface is also effective. Furthermore, after exhaust is completed and the air inlet 310 is separated from the reaction vessel 116v, continuing to draw in air is also an effective measure.

[0033] Give examples to illustrate the method of adjusting evaporation amount. Figure 4 is Figure 3A 、 Figure 3B The results of evaporation and concentration are shown for the evaporation and concentration mechanism 131 when it is not connected to the atmospheric introduction tube and when it is connected. The experiment was conducted under the same conditions except for the connection of the atmospheric introduction tube. Furthermore, the evaporation rate on the vertical axis represents the ratio of the volume of the evaporated sample to the volume of the sample before evaporation and concentration. In this example, the evaporation rate is approximately 60% when the atmospheric introduction tube is not connected. In contrast, the evaporation rate is less than 80% when the atmospheric introduction tube is connected, indicating an increase in the evaporation rate.

[0034] Figure 5 It is the result of implementing evaporation concentration by changing the length of the connected atmospheric introduction pipe. The vertical axis represents the average value of the evaporation rate obtained by conducting multiple experiments. In this experiment, atmospheric introduction pipes of four lengths, 100mm, 400mm, 800mm, and 1030mm, were connected. It can be seen that the average evaporation rate is the highest when the atmospheric introduction pipe is the shortest, 100mm. The longer the atmospheric introduction pipe, the lower the average evaporation rate. Therefore, it can be seen that the evaporation rate can be adjusted by adjusting the length of the atmospheric introduction pipe. It can be considered that the change in evaporation rate caused by the length of the atmospheric introduction pipe depends on the atmospheric inflow rate of the atmosphere flowing into the atmospheric introduction pipe per unit time. Therefore, the evaporation rate can be adjusted not only by changing the length of the atmospheric introduction pipe, but also by changing the diameter of the atmospheric introduction pipe, the position and number of the atmospheric introduction parts, etc. In addition, the evaporation rate can be adjusted by combining these adjustment methods for adjusting the atmospheric inflow rate.

[0035] Figure 6 Yes Figure 5 The relationship between the length of the atmospheric inlet pipe and the flow rate in the experiment shown is the result of theoretical calculation. Figure 3B In the exhaust section 320 shown, a pressure gauge is arranged in the exhaust pipe between the atmosphere inlet section 322 and the exhaust mechanism (vacuum pump) 321 to measure the pressure at the inlet of the exhaust mechanism 321. Using the characteristic line diagram of the vacuum pump (exhaust mechanism, negative pressure source) showing the relationship between the vacuum degree and the air flow rate, the total flow rate is calculated based on the value of the pressure gauge as the air flow rate discharged by the exhaust mechanism. The flow rate on the atmospheric inlet pipe side, i.e., the air flow rate flowing in from the atmospheric inlet pipe 323, and the flow rate on the intake side, i.e., the amount of air flowing in from the intake section 310, are calculated as follows. The pressure loss of the atmospheric inlet pipe 323 can be calculated by theoretical calculation. On the other hand, the intake section 310 and the exhaust mechanism 321 are directly connected and the pressure loss of the intake section 310 is calculated based on the exhaust result. Based on the pressure loss between the atmospheric inlet pipe 323 and the intake section 310 calculated in this way, the flow balance is calculated to obtain the atmospheric inlet pipe side flow rate and the intake section side flow rate.

[0036] from Figure 5 It can be seen that the evaporation rate is higher when the 100mm and 400mm atmospheric introduction pipes are connected than when there is no introduction pipe. In addition, the evaporation rate tends to decrease as the length of the atmospheric introduction pipe becomes longer. Figure 6 As can be seen, the total flow rate decreases as the length of the atmospheric introduction pipe increases, showing a trend similar to the evaporation rate. However, looking at the details of the total flow rate, the flow rate on the atmospheric introduction pipe side decreases as the total flow rate decreases. Conversely, the flow rate on the air intake side is smaller when the 100mm atmospheric introduction pipe is connected than when the 400mm atmospheric introduction pipe is connected, showing a trend opposite to the total flow rate or the flow rate on the atmospheric introduction pipe side.

[0037] Figure 7 This is a schematic diagram of steam being drawn in through the air intake 310. When exhaust begins through the exhaust mechanism 321, outside air flows in through the through-hole 312 provided in the arm 311. This influx of outside air through the through-hole 312 generates airflow within the reaction vessel 116v, which shakes the liquid level of the refined liquid 330, thereby increasing the surface area of ​​the refined liquid 330 in contact with the outside air. This change in surface area is believed to affect the evaporation rate of the refined liquid 330. In this embodiment, it is believed that by connecting a relatively short atmospheric air intake pipe, the majority of the total flow rate discharged by the exhaust mechanism 321 is provided by outside air from the atmospheric air intake pipe, thereby reducing the flow rate on the air intake side. This allows the outside air flowing in through the through-hole 312 to be efficiently drawn in as airflow within the vessel.

[0038] As described above, according to this embodiment, by varying the length of the atmospheric air inlet tube, the amount of outside air flowing into the air intake portion can be adjusted, thereby regulating the amount of evaporation from the reaction vessel. Furthermore, depending on the installation environment, the evaporation rate of an automatic analyzer equipped with this evaporation and concentration mechanism may sometimes exceed the designed value. In such cases, an automatic analyzer can be implemented that can achieve a predetermined evaporation rate by varying the length and / or diameter of the atmospheric air inlet tube.

[0039] In addition, the present invention is not limited to the above-mentioned embodiments and modifications, but also includes various other modifications. For example, the above-mentioned embodiments are detailed descriptions for the purpose of explaining the present invention in an easy-to-understand manner, and the present invention is not necessarily limited to including all the structures described. For example, in the above-mentioned examples, an example of performing concentration processing by heat treatment and exhaust treatment is shown, but an example of performing concentration processing by exhaust treatment alone is also possible. Even if only exhaust treatment is performed, concentration processing of the sample can be performed. In addition, the analysis unit 103 can be an optical analysis device other than a mass spectrometer, for example. Label Description

[0040] 100 Automatic analyzer, 101 Pretreatment unit, 102 Separation unit, 103 Analysis unit, 104 Control unit, 111 Sample container, 112 Conveying mechanism, 113 Sample dispensing mechanism, 114 Dispensing needle mounting and detaching unit, 115 Dispensing needle mounting rack, 115a Dispensing needle, 116, 116v Reaction container, 117 Reaction container mounting rack, 118 Conveying mechanism, 119 Opening, 120 Incubator, 121 Reagent container, 122 Reagent tray, 123 Reagent dispensing mechanism, 124 4 Magnetic separation mechanism, 125 Conveying mechanism, 126 Rotating track, 131 Evaporation and concentration mechanism, 132 Conveying mechanism, 133 Dispensing mechanism for separation section, 201 Magnet, 202 Magnetic bead group, 203 Magnetic bead group, 301 Container storage section, 302 Container setting section, 303 Heating section, 310 Air inlet section, 311 Arm, 312 Through hole, 313 Support, 314 Exhaust flow path, 320 Exhaust section, 321 Exhaust mechanism, 322 Atmosphere introduction section, 323 Atmosphere introduction pipe, 324 Exhaust pipe.

Claims

1. An evaporation and concentration mechanism for evaporating and concentrating a liquid contained in a reaction container, characterized in that: include: a container setting portion, the container setting portion being used to set the reaction container; an air inlet portion capable of contacting the reaction container in a manner covering an opening of the reaction container; and an exhaust portion for exhausting the gas in the reaction container via the gas inlet portion, The air intake portion includes an exhaust flow path, one end of which is connected to the exhaust portion, and the other end of which is connected to the opening of the reaction container through the air intake portion contacting the reaction container; and a through hole, which allows external air to flow into the reaction container. The exhaust portion includes a negative pressure generating source for generating negative pressure, an exhaust pipe connecting the negative pressure generating source and the exhaust flow path of the intake portion, and an atmosphere introduction portion for introducing atmosphere into the exhaust pipe, wherein the atmosphere introduction pipe in the atmosphere introduction portion is detachable.

2. The evaporation concentration mechanism according to claim 1, wherein: The atmospheric air introduction pipe may be selected from a plurality of atmospheric air introduction pipes having different lengths and / or inner diameters and may be installed in the atmospheric air introduction portion.

3. The evaporation concentration mechanism according to claim 1, wherein: The atmosphere introduction portion is a branch portion of the exhaust pipe, or a block provided with holes for gas circulation.

4. The evaporation concentration mechanism according to claim 1, wherein: The exhaust portion includes a plurality of the atmosphere introduction portions.

5. The evaporation concentration mechanism according to claim 1, wherein: A heating unit is provided on the container installation portion and heats the reaction container. The reaction container is heated by the heating portion, and the gas in the reaction container is exhausted through the gas inlet portion, thereby evaporating and concentrating the liquid contained in the reaction container.

6. An automatic analysis device comprising the evaporation and concentration mechanism according to any one of claims 1 to 5, characterized in that: An analysis unit is included that analyzes the liquid contained in the reaction container after being concentrated by the evaporation and concentration mechanism.

7. The automatic analyzer according to claim 6, wherein The atmospheric air introduction pipe selected according to the evaporation rate specification determined for the evaporation concentration mechanism is connected to the evaporation concentration mechanism.

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