Anti-pollution compound and application thereof in cosmetics

Abstract

The invention discloses an anti-pollution compound and application thereof in cosmetics, and belongs to the technical field of cosmetic raw materials. The active ingredients of the anti-pollution compound comprise the tea extract, the polygonum multiflorum root extract and the chrysosplenium nigrum seed extract, and the compound has an excellent anti-oxidation effect by utilizing the combined action of the active ingredients, and has the functions of inhibiting protein and nucleic acid damage in ozone and nitrogen dioxide and protecting cells; and the skin can be effectively protected from being damaged by gaseous pollutants in the air when being used in cosmetics.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetic raw materials, and particularly relates to an anti-pollution compound and application thereof in cosmetics. Background Art

[0002] In recent years, with the acceleration of industrialization and the surge in motor vehicle ownership, concentrations of oxidative pollutants such as surface ozone (O3) and nitrogen dioxide (NO2) have shown a significant upward trend. According to the World Health Organization's 2023 Global Air Quality Report, daily average O3 concentrations in urban areas have risen 37% since the end of the 20th century, and the number of days with NO2 exceeding the standard has increased 2.8-fold. These gaseous pollutants can penetrate the skin through both epidermal penetration and hair follicle adsorption, triggering a series of pathological changes. Firstly, they induce the production of reactive oxygen species (ROS) in keratinocytes, leading to abnormal activation of the nuclear factor κB (NF-κB) signaling pathway and accelerated matrix metalloproteinase (MMP)-mediated collagen degradation. Secondly, they react with squalene in sebum to produce highly inflammatory peroxidases, disrupting the skin barrier function. Extensive experimental data also confirm that O3 and NO2 cause lipid peroxidation in the skin, damage proteins and nucleic acids, trigger inflammation, reduce cell survival, and cause adverse changes in cell morphology.

[0003] Existing anti-pollution cosmetics mostly adopt a passive protection strategy of physical isolation (such as film-forming agents) or single antioxidants (such as vitamin C derivatives), which have significant technical defects: (1) Although silicon-based film-forming agents can block particulate matter such as PM2.5, their blocking efficiency for gaseous pollutants is less than 40%; (2) Traditional antioxidants have difficulty in achieving multi-target regulation in the complex oxidative stress network induced by pollutants, and have problems such as low transdermal absorption rate (<15%) and poor photostability. Therefore, the development of a multi-dimensional anti-pollution system with an active defense mechanism for the skin, especially an innovative formula that can simultaneously remove the effects of gaseous pollutants, block oxidative signal transduction, and repair the skin barrier function, has become a technical problem that needs to be solved urgently in the cosmetics field.

[0004] Natural plant extracts as cosmetic raw materials have the characteristics of natural mildness, high safety, antioxidant, moisturizing, soothing and repairing. Chinese patent CN118453482A discloses a method for preparing anti-pollution cosmetics. In addition to conventional ingredients, the anti-pollution cosmetics are also compounded with functional ingredients such as Morus alba extract, Rhodiola rosea extract, Quercus robur root extract, Leontopodium alpinum extract, and Dendrobium officinale extract. They can protect skin cell activity and reduce skin damage caused by ultraviolet radiation; however, this method can only prevent the damage of light pollution and cannot fundamentally and effectively eliminate skin problems caused by pollutants such as O3 and NO2 in the air. Chinese CN106176468A uses active ingredients such as burdock extract, Eucommia ulmoides extract, black pepper extract, and deep-sea fish collagen to calm and protect skin that has become sensitive due to environmental factors; however, this patented formula can only solve the harm of benzopyrene to the skin, and its protective effect on the skin is very limited. Summary of the Invention

[0005] In order to solve the problem of skin aging, pigmentation and various skin diseases caused by the significant increase in the concentration of gaseous pollutants such as ozone and nitrogen dioxide in the atmosphere, the present invention provides an anti-pollution complex that can effectively protect the skin from gaseous pollutants in the air, thereby inhibiting lipid oxidation, preventing skin damage, and defending against cytotoxicity.

[0006] The effect of the present invention is achieved by the following means:

[0007] First, the present invention provides an anti-pollution compound, the active ingredients of which include CAMELLIA SINENSIS leaf extract, POLYGONUM MULTIFLORUM root extract and CUSCUTA JAPONICA seed extract.

[0008] Preferably, the anti-pollution compound has the ability to scavenge superoxide radicals and hydroxyl radicals.

[0009] Preferably, the anti-pollution complex at a mass concentration of 2% has a superoxide radical scavenging rate of ≥89.0%.

[0010] Preferably, the anti-pollution compound has the ability to inhibit protein and nucleic acid damage in ozone and nitrogen dioxide.

[0011] Preferably, the anti-pollution compound has the function of protecting cells from ozone and nitrogen dioxide.

[0012] Preferably, the anti-pollution compound comprises tea leaf extract, Polygonum multiflorum root extract, Cnidium monnieri seed extract and a solvent.

[0013] Preferably, the solvent includes but is not limited to at least one of water, ethanol, propylene glycol, glycerol, butylene glycol, hexylene glycol, cetyl alcohol, octadecyl alcohol, ethyl acetate, isopropyl palmitate, isooctyl palmitate, and caprylic / capric glyceride.

[0014] Preferably, the solvent comprises water and / or butanediol.

[0015] Preferably, the anti-pollution compound comprises the following components in mass fractions: 2-5% tea leaf extract, 2-5% Polygonum multiflorum root extract, 0.5-1% Caulis et Rhizoma Coptidis seed extract, and the solvent is added to make up the remainder to 100%.

[0016] Preferably, the tea extract includes but is not limited to at least one of green tea extract, black tea extract, oolong tea extract, and white tea extract.

[0017] Preferably, the tea leaf extract comprises green tea leaf extract.

[0018] Preferably, the anti-pollution compound comprises the following components by mass fraction: 2-5% tea leaf extract, 2-5% Polygonum multiflorum root extract, 0.5-1% Caulis et Rhizoma Coptidis seed extract, 10-55% butylene glycol, and the balance is made up of water to 100%.

[0019] Preferably, the mass ratio of the tea leaf extract, the Polygonum multiflorum root extract and the Caulis Adenophorae flavescentis seed extract is 1:0.4-2.5:0.1-0.5.

[0020] In a preferred embodiment, the mass ratio of the tea leaf extract, the Polygonum multiflorum root extract and the Celastrus australis seed extract is 1:0.7-1.2:0.2-0.35.

[0021] Preferably, the pH value of the anti-pollution complex is 4.5-7.5 (20° C., 10% solution).

[0022] Preferably, the present application also provides the use of the anti-pollution complex in cosmetics, and the cosmetics include but are not limited to any one of cleansing milk, cleansing foam, cleansing soap, cleansing powder, cleansing gel, cleansing cream, makeup remover oil, makeup remover water, makeup remover milk, makeup remover cream, makeup remover gel, toner, softener, toner, lotion, cream, essence water, essence liquid, essence oil, sunscreen, sunscreen milk, sunscreen spray, sheet mask, smear mask, mud mask, eye cream, eye mask, etc.

[0023] Beneficial effects of the present invention:

[0024] (1) The active ingredients of the present invention include tea extract, Polygonum multiflorum root extract and Cnidium monnieri seed extract, which are of natural origin and highly safe. By optimizing the content of the three ingredients and utilizing the synergistic effect of the active ingredients, the prepared anti-pollution complex has excellent antioxidant activity, can effectively inhibit reactive oxygen free radicals and lipid oxidation, prevent skin damage, and defend against cytotoxicity. As a cosmetic raw material, it can protect the skin from the invasion of gaseous pollutants in the air.

[0025] (2) By optimizing the relative amounts of active ingredients and the types of solvents, the present invention not only enables the active ingredients to fully exert their effects, but also ensures the balance of molecular forces within the system and the stability of the complex when stored in different environments. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 : Comparison chart of electrophoresis test of the anti-pollution complex in Example 1 to inhibit ozone damage to proteins.

[0027] Figure 2 : Comparison diagram of electrophoresis test of the anti-pollution complex in Example 1 to inhibit protein damage caused by nitrogen dioxide.

[0028] Figure 3 : Comparison chart of electrophoresis test of the anti-pollution complex in Example 1 to inhibit damage of nucleic acid by nitrogen dioxide. DETAILED DESCRIPTION

[0029] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0030] The term "extract" includes all substances obtained by extracting components of natural products, regardless of the extraction method, extraction solvent, extraction conditions, extracted components, or the form of the extract. It can also include substances obtained through processing or treatment; such processing or treatment may include dilution, concentration, drying, purification, classification, filtration, fermentation, enzyme treatment, etc. Therefore, in this specification, extract refers to the extract, the diluted or concentrated extract, the dried product obtained by drying the extract, its crude or refined product, the fraction obtained by fractionation, and the filtrate obtained by drying. Primary and / or secondary filtration may be included.

[0031] First, the present invention provides an anti-pollution compound, the active ingredients of which include CAMELLIA SINENSIS leaf extract, POLYGONUM MULTIFLORUM root extract and CUSCUTA JAPONICA seed extract.

[0032] Green tea is known to benefit human health, and it also offers numerous benefits for skin and hair. In the Compendium of Materia Medica, tea is classified as a "grain" and is believed to have medicinal properties such as clearing heat and detoxifying, refreshing the mind, anti-tumor, and antibacterial and anti-inflammatory. Tea extracts are rich in active ingredients such as tea polyphenols, catechins, vitamin C, and vitamin A. These ingredients possess potent antioxidant properties, neutralizing free radicals and reducing oxidative damage to the skin caused by environmental pollutants. They offer antioxidant, cell regeneration, whitening, anti-inflammatory, soothing, and antibacterial properties (for dermatitis, eczema, and rashes). Consequently, they are widely used in cosmetics.

[0033] "Compendium of Materia Medica" records: During the reign of Emperor Wu of Han, there was a horse liver stone that could blacken people's hair, so later generations concealed this name (He Shou Wu), and also called it horse liver stone. Li Ao of the Tang Dynasty wrote in "The Biography of He Shou Wu": "The seedlings of He Shou Wu are as shiny as straw, shaped like peach and willow leaves, with their backs tilted, solitary, and all grow without facing each other. There are male and female ones, the female seedlings are yellow and white, and the male ones are yellow and red. They grow far apart, and at night the seedlings intertwine or disappear. The recipe of Qibao Mei Ran Dan (blackening hair, strengthening muscles and bones, and consolidating essence and qi) in "Jishan Tang Experience Prescription" uses red and white He Shou Wu. "Chinese Pharmacopoeia" records: He Shou Wu has the functions of detoxification, eliminating carbuncle, stopping malaria, moistening the intestines and relieving constipation, and is mainly used to treat sores Carbuncle, scrofula, urticaria itching, long-term malaria weakness, dry intestine constipation, etc. The root extract of Polygonum multiflorum contains a variety of active ingredients such as diphenyl glycosides (such as 2,3,5,4'-tetrahydroxyphenyl 2-O-β-D-glucoside), lecithin, emodin, chrysophanol, rhein, physcion methyl ether, etc. These ingredients give the root extract of Polygonum multiflorum its antioxidant, moisturizing, anti-inflammatory and soothing properties, regulating skin metabolism, and enhancing skin immunity.

[0034] The main components of the golden lantern vine seed extract are β-carotene, γ-carotene, 5,6-epoxy-α-carotene, taraxanthin and lutein, including rich polyphenol compounds (such as catechins, flavonoids, etc.), alkaloids and organic acids (such as palmitic acid, saturated fatty acid mixture, stearic acid, arachidic acid, p-hydroxycinnamic acid, caffeic acid, etc.) active ingredients, which have antioxidant, anti-inflammatory, soothing, antibacterial, and barrier repair promoting effects.

[0035] Preferably, the anti-pollution compound has the ability to scavenge superoxide radicals and hydroxyl radicals.

[0036] Preferably, the anti-pollution complex at a mass concentration of 2% has a superoxide radical scavenging rate of ≥89.0%.

[0037] Preferably, the anti-pollution compound has the ability to inhibit protein and nucleic acid damage in ozone and nitrogen dioxide.

[0038] Preferably, the anti-pollution compound has the function of protecting cells from ozone and nitrogen dioxide.

[0039] Preferably, the anti-pollution compound comprises tea leaf extract, Polygonum multiflorum root extract, Cnidium monnieri seed extract and a solvent.

[0040] Preferably, the solvent includes but is not limited to at least one of water, ethanol, propylene glycol, glycerol, butylene glycol, hexylene glycol, cetyl alcohol, octadecyl alcohol, ethyl acetate, isopropyl palmitate, isooctyl palmitate, and caprylic / capric glyceride.

[0041] Preferably, the solvent comprises water and / or butanediol.

[0042] Preferably, the anti-pollution compound comprises the following components in mass fractions: 2-5% tea leaf extract, 2-5% Polygonum multiflorum root extract, 0.5-1% Caulis et Rhizoma Coptidis seed extract, and the solvent is added to make up the remainder to 100%.

[0043] Preferably, the tea extract includes, but is not limited to, at least one of green tea leaf extract, black tea leaf extract, oolong tea leaf extract, and white tea leaf extract. Compared to black tea leaf extract, oolong tea leaf extract, or white tea leaf extract, green tea leaf extract has higher levels of tea polyphenols and catechins, effectively scavenging free radicals and inhibiting free radical reactions, exhibiting stronger antioxidant capacity and more effectively slowing skin aging.

[0044] Preferably, the tea extract comprises green tea leaf extract. The anti-pollution compound may optionally contain any one of 2.0%, 2.1%, 2.2%, 2.3%, 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 2.9%, 3.0%, 3.1%, 3.2%, 3.3%, 3.4%, 3.5%, 3.6%, 3.7%, 3.8%, 3.9%, 4.0%, 4.1%, 4.2%, 4.3%, 4.4%, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%, 5.0% or the like tea extract by mass fraction.

[0045] Preferably, the anti-pollution complex optionally contains 2.0%, 2.05%, 2.1%, 2.15%, 2.2%, 2.25%, 2.3%, 2.35%, 2.4%, 2.45%, 2.5%, 2.55%, 2.6%, 2.65%, 2.7%, 2.75%, 2.8%, 2.85%, 2.9%, 3.0%, 3.05%, 3.1%, 3.2%, 3.3%, 3.4%, 3.5%, 3.6%, 3.7%, 3.8%, 3.9%, 4.0%, 4.1%, 4.2%, 4.3%, 4.4%, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%, 5.0%, etc., any one of the mass fractions of Polygonum multiflorum root extract.

[0046] Preferably, the anti-pollution complex optionally contains any one of 0.5%, 0.52%, 0.54%, 0.55%, 0.57%, 0.59%, 0.6%, 0.61%, 0.63%, 0.65%, 0.67%, 0.69%, 0.7%, 0.72%, 0.74%, 0.75%, 0.76%, 0.78%, 0.8%, 0.81%, 0.83%, 0.85%, 0.87%, 0.89%, 0.9%, 0.92%, 0.94%, 0.95%, 0.97%, 0.99%, 1.0% or the like mass fraction of the extract of the seeds of the Chinese lantern vine.

[0047] Preferably, the anti-pollution compound comprises the following components by mass fraction: 2-5% tea leaf extract, 2-5% Polygonum multiflorum root extract, 0.5-1% Caulis et Rhizoma Coptidis seed extract, 10-55% butylene glycol, and the balance is made up of water to 100%.

[0048] Preferably, the mass ratio of the tea extract, Polygonum multiflorum root extract and Caulis et Rhizoma Coptidis seed extract is 1:0.4-2.5:0.1-0.5; by specifying the relative contents of the three, the optimal stability of the complex can be obtained. When the components are inappropriate, the stability of the system may be unbalanced; it may be because the different chemical components in the three extracts will undergo complexation reactions. It is speculated that when the composition of the tea extract is too high, it will directly lead to an excessively high content of alkaloids in the composition, prompting the above alkaline substances to form complexes with certain acidic components in the Polygonum multiflorum root extract or the Caulis et Rhizoma Coptidis seed extract, affecting the clarity and stability of the solution, and may even cause the formation of precipitation. It may also be because the polarity of the components contained in the three extracts is different. Only under the above ratio can appropriate intermolecular interactions occur between the components to jointly maintain the balance of the system's forces. When the content of at least one of the extracts is too high, the polarity difference between the components in the system is too large, resulting in phase separation. It is speculated that when the tea polyphenols and other highly polar components in the tea extract of the composition are mixed with the excessive and less polar oil components in the golden lantern vine seed extract, stratification and other problems are likely to occur.

[0049] In a preferred embodiment, the mass ratio of the tea leaf extract, the Polygonum multiflorum root extract and the Celastrus australis seed extract is 1:0.7-1.2:0.2-0.35.

[0050] Preferably, the tea extract, Polygonum multiflorum root extract and Cnidium monnieri seed extract can be obtained by at least one of water extraction, organic solvent extraction, supercritical fluid extraction, microwave-assisted extraction, ultrasound-assisted extraction and enzymatic extraction.

[0051] Preferably, the pH value of the anti-pollution compound is 4.5-7.5 (20° C., 10% solution); the above pH range can ensure that the components in the compound have an appropriate degree of hydrolysis without adversely affecting the efficacy and stability of the compound.

[0052] Preferably, the anti-pollution complex may further include conventional cosmetic ingredients, including but not limited to moisturizers, thickeners, preservatives, pH regulators, fragrances, pigments, surfactants, oils, waxes, and the like.

[0053] Preferably, the moisturizer includes, but is not limited to, at least one of glycerin, sodium hyaluronate, butylene glycol, propylene glycol, petrolatum, petrolatum, squalane, amino acid moisturizers, and sodium PCA. The thickener includes, but is not limited to, at least one of carbomer, xanthan gum, guar gum, gum arabic, polyacrylamide and its derivatives, hydroxyethyl cellulose, hydroxypropyl cellulose, and bentonite. The preservative includes, but is not limited to, at least one of phenoxyethanol, methylparaben, ethylparaben, propylparaben, potassium sorbate, sodium benzoate, rosemary extract, imidazolidinyl urea, and DMDM ​​hydantoin. The surfactant includes, but is not limited to, at least one of sodium laureth sulfate, sodium dodecylbenzenesulfonate, cocamidopropyl betaine, cocamidopropyl hydroxysultaine, polysorbate 20, and PEG-4 hydrogenated castor oil.

[0054] Preferably, the anti-pollution complex is used in cosmetics, including but not limited to any one of cleansing milk, cleansing foam, cleansing soap, cleansing powder, cleansing gel, cleansing cream, makeup remover oil, makeup remover water, makeup remover milk, makeup remover cream, makeup remover gel, toner, softener, toner, lotion, cream, essence water, essence liquid, essence oil, sunscreen, sunscreen milk, sunscreen spray, sheet mask, smear mask, mud mask, eye cream, eye mask, etc.

[0055] Example 1

[0056] This embodiment provides an anti-pollution complex. The anti-pollution complex comprises, by total mass, 2.5% of green tea leaf extract (CAS: 84650-60-2), 2.5% of Polygonum multiflorum root extract (CAS: 478-43-3), 0.8% of Celastrus australis seed extract (CAS: 89998-10-7), 40.0% of butylene glycol, and the remainder being 100% water.

[0057] The preparation method of the anti-pollution complex comprises the following steps: mixing green tea leaf extract, Polygonum multiflorum root extract, Cnidium monnieri seed extract, butylene glycol and water, stirring the mixture evenly, wherein the pH value is 5.3 at room temperature; and the mixture is stored at room temperature away from direct sunlight.

[0058] Example 2

[0059] This embodiment provides an anti-pollution complex. The anti-pollution complex comprises, by total mass, 5% green tea leaf extract (CAS: 84650-60-2), 2% Polygonum multiflorum root extract (CAS: 478-43-3), 0.5% Aconitum carmichaelii seed extract (CAS: 89998-10-7), 50.0% butylene glycol, and the remainder being 100% water.

[0060] The preparation method was the same as that in Example 1, and the pH value was 5.5 at room temperature.

[0061] Example 3

[0062] This embodiment provides an anti-pollution complex. The anti-pollution complex comprises, by total mass, 2% green tea leaf extract (CAS: 84650-60-2), 5% Polygonum multiflorum root extract (CAS: 478-43-3), 1% Aconitum quinata seed extract (CAS: 89998-10-7), 30.0% butylene glycol, and the remainder being 100% water.

[0063] The preparation method was the same as that in Example 1, and the pH value was 4.8 at room temperature.

[0064] Example 4

[0065] This embodiment provides an anti-pollution complex. The anti-pollution complex comprises, by total mass, 2.5% of green tea leaf extract (CAS: 84650-60-2), 2.5% of Polygonum multiflorum root extract (CAS: 478-43-3), 0.8% of Celastrus australis seed extract (CAS: 89998-10-7), 40.0% of hexadecanol, and the remainder being 100% water.

[0066] The preparation method was the same as that in Example 1, and the pH value was 5.2 at room temperature.

[0067] Example 5

[0068] This embodiment provides an anti-pollution complex. The anti-pollution complex comprises, by total mass, 2.5% of green tea leaf extract (CAS: 84650-60-2), 2.5% of Polygonum multiflorum root extract (CAS: 478-43-3), 0.8% of Celastrus nobilis seed extract (CAS: 89998-10-7), and the remainder being 100% by weight of water.

[0069] The preparation method was the same as that in Example 1, and the pH value was 5.3 at room temperature.

[0070] Comparative Example 1

[0071] This comparative example provides an anti-pollution compound. The anti-pollution compound comprises, by total mass, 5.8% of green tea leaf extract, 40.0% of butanediol, and the balance of water being 100%.

[0072] Comparative Example 2

[0073] This comparative example provides an anti-pollution compound. The anti-pollution compound comprises, by total mass, 2.9% of Polygonum multiflorum root extract, 2.9% of Celastrus australis seed extract, 40.0% of butylene glycol, and the remainder being 100% by water.

[0074] Comparative Example 3

[0075] This comparative example provides an anti-pollution complex. The anti-pollution complex comprises, by total mass, 1.0% green tea leaf extract, 4.0% Polygonum multiflorum root extract, 0.8% Celastrus australis seed extract, 40.0% butylene glycol, and the remainder being 100% water.

[0076] Comparative Example 4

[0077] This comparative example provides an anti-pollution complex. The anti-pollution complex comprises, by total mass, 4.0% green tea leaf extract, 1.0% Polygonum multiflorum root extract, 0.8% Celastrus australis seed extract, 40.0% butylene glycol, and the remainder being 100% water.

[0078] Performance testing method:

[0079] 1. Antioxidant efficacy test:

[0080] (1) Superoxide anion (O2 - ) Free radical scavenging experiment

[0081] Take 4.5mL of 0.05mol / L Tris-HCl buffer (pH=8.2) in a 15mL centrifuge tube and preheat it in a 25℃ water bath for 20min. Add 2% mass concentration of the sample solution to be tested, then add 0.4mL of 25mmol / L pyrogallol solution. After mixing, react accurately for 4min in a 25℃ water bath. Immediately add 1mL of concentrated HCl to terminate the reaction. Finally, dilute to 10mL with distilled water and measure the absorbance A at 325nm.

[0082] The calculation formula of superoxide anion radical scavenging rate is as follows:

[0083] Superoxide anion radical scavenging rate % = [1-(A sample-A sample blank) / A blank]*100%;

[0084] Where: A blank—add pyrogallol but no sample;

[0085] Sample A—add pyrogallol and sample;

[0086] A. Sample blank—no pyrogallol is added but sample is added.

[0087] (2) Hydroxyl free radical scavenging experiment

[0088] Add 2 mL of 2 mmol / L FeSO4 solution, 2 mL of 6 mmol / L salicylic acid-ethanol solution, 2 mL of 2% mass concentration of the sample solution, and 1 mL of 0.03% H2O2 in sequence, shake well, dilute to 10 mL with distilled water, react in a 37°C water bath for 30 min, and measure the absorbance A at 510 nm.

[0089] The calculation formula of hydroxyl radical scavenging rate is as follows:

[0090] Hydroxyl radical scavenging rate % = (A0-Ax) / A0*100%;

[0091] Where A0 is the absorbance value of the reagent blank; Ax is the absorbance value of the sample solution.

[0092] (3) Determination of relative expression of lipid peroxidation product MDA

[0093] Human skin keratinocytes (HaCaT) in the logarithmic growth phase were seeded into 6-well plates at 5×105 cells / well and placed in a CO2 incubator for 24 hours for cell attachment. The experiment set up a blank control group, a model control group, and a sample group, with 3 replicates in each group. Normal cell culture medium was added to the blank control group, culture medium containing LPS was added to the model control group, and culture medium containing 2% mass concentration of the test sample and LPS was added to the sample group, and the cells were cultured in a CO2 incubator for 24 hours. The culture medium was discarded, 100L of lysis buffer was added to each well to lyse the cells, and the supernatant was centrifuged at 4°C and operated according to the human malondialdehyde (MDA) ELISA kit.

[0094] By comparing the MDA levels of the model group and the sample group, the inhibition rate was calculated:

[0095] MDA inhibition rate %=(1-MDA content in sample group / MDA content in model group)*100%.

[0096] The antioxidant efficacy test results of the above examples and comparative examples are shown in Table 1.

[0097] Table 1

[0098]

[0099]

[0100] From the above results, it can be seen that when tea extract, Polygonum multiflorum root extract and Caulis et Rhizoma Coptidis seed extract are used in combination in Examples 1-5, the combined effect of the three can be utilized to obtain better antioxidant properties and protective effects on skin proteins and cells, and effectively resist the damage caused by ozone and nitrogen dioxide to the skin.

[0101] 2. Protein damage inhibition test: Albumin was treated with 11 ppm ozone or nitrogen dioxide gas for 20 min, and the SDS-PAGE (polyacrylamide gel electrophoresis) method was used to determine the protein damage inhibition of the anti-pollution composite of Example 1 in ozone (attached Figure 1 ) or nitrogen dioxide (with Figure 2 ) inhibits protein damage.

[0102] Attachment Figure 1In the experiment, 1 is a blank control experiment, 2 is an ozone treatment experiment, 3 is an ozone + 1% mass concentration anti-pollution compound treatment experiment, and 4 is an ozone + 2% mass concentration anti-pollution compound treatment experiment. Figure 1 It can be seen that the group treated with the anti-pollution complex exhibited an excellent effect of inhibiting protein damage in a concentration-dependent manner compared with the control group not treated with ozone.

[0103] Attachment Figure 2 In the experiment, 1 is a blank control experiment, 2 is a nitrogen dioxide treatment experiment, 3 is a nitrogen dioxide + 1% mass concentration anti-pollution compound treatment experiment, and 4 is a nitrogen dioxide + 2% mass concentration anti-pollution compound treatment experiment. Figure 2 It can be seen that compared with the control group not treated with nitrogen dioxide, the group treated with the anti-pollution complex showed a better effect of inhibiting protein damage, and it also showed concentration dependence.

[0104] 3. Nucleic acid damage inhibition test: The nucleic acids isolated from melanocytes B16F10 were exposed to 10 ppm nitrogen dioxide, and the degree of damage was determined by nucleic acid electrophoresis (when DNA is damaged, the fluorescence of the band will decrease due to impaired electrophoretic mobility or due to interaction with fluorescent materials due to changes in its structure).

[0105] Attachment Figure 3 Among them, 1 is a blank control experiment (without nitrogen dioxide treatment), 2 is an experiment of nitrogen dioxide + 1% mass concentration anti-pollution compound treatment, and 4 is an experiment of nitrogen dioxide + 2% mass concentration anti-pollution compound treatment. Figure 3 As can be seen from the comparison, the extent of the nucleic acid bands treated with the anti-pollution complex is similar to that of the nucleic acid bands not treated with nitrogen dioxide, indicating that the anti-pollution complex of Example 1 has the effect of inhibiting nucleic acid damage.

[0106] 4. Stability test: The test samples were stored at -10°C, 4°C, 25°C, and 45°C for 3 months. The product appearance was observed regularly (at 1 week, 2 weeks, 3 weeks, 1 month, 2 months, and 3 months), and the physical state, color, and odor were recorded. The experimental results are shown in Table 2.

[0107] Table 2

[0108]

[0109]

[0110] As can be seen from Table 2 above, when the mass ratio of tea extract, Polygonum multiflorum root extract and Caulis et Rhizoma Coptidis seed extract in the composite is controlled to be 1:0.4-2.5:0.1-0.5 and combined with a specific solvent, a better system stability can be obtained, which complies with the regulations of the cosmetics industry.

[0111] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus.

[0112] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. An anti-pollution composite, characterized in that Active ingredients include tea leaf extract, Polygonum multiflorum root extract, and Cnidium officinale seed extract.

2. The anti-pollution composite according to claim 1, characterized in that The anti-pollution complex at a concentration of 2% by mass had a superoxide radical scavenging rate of ≥89.0%.

3. The anti-pollution composite according to claim 1, characterized in that The anti-pollution complex has the ability to inhibit protein and nucleic acid damage in ozone and nitrogen dioxide.

4. The anti-pollution composite according to claim 1, characterized in that The anti-pollution complex has the function of protecting cells from ozone and nitrogen dioxide.

5. The anti-pollution composite according to claim 1, characterized in that The components of the anti-pollution complex include: tea extract, Polygonum multiflorum root extract, Celastrus australis seed extract and a solvent; the solvent includes at least one of water, ethanol, propylene glycol, glycerol, butylene glycol, hexylene glycol, hexadecanol, octadecyl alcohol, ethyl acetate, isopropyl palmitate, isooctyl palmitate and caprylic / capric glyceride.

6. The anti-pollution composite according to claim 5, characterized in that Solvents include water and / or butylene glycol.

7. The anti-pollution composite according to claim 5, characterized in that The anti-pollution compound comprises the following components in mass fractions: 2-5% of tea extract, 2-5% of Polygonum multiflorum root extract, 0.5-1% of Caulis et Rhizoma Coptidis seed extract, and the remainder is supplemented with solvent to 100%.

8. The anti-pollution composite according to claim 1, characterized in that The tea extract includes at least one of green tea leaf extract, black tea leaf extract, oolong tea leaf extract, and white tea leaf extract, and preferably includes green tea leaf extract.

9. The anti-pollution composite according to claim 1, characterized in that The mass ratio of the tea leaf extract, the Polygonum multiflorum root extract and the Caulis Adenophorae flavescentis seed extract is 1:0.4-2.5:0.1-0.

5.

10. Use of the anti-pollution complex according to claims 1-9 in cosmetics, characterized in that: The cosmetics include any one of cleansing milk, cleansing foam, cleansing soap, cleansing powder, cleansing gel, cleansing cream, makeup remover oil, makeup remover water, makeup remover milk, makeup remover cream, makeup remover gel, toner, softener, toner, lotion, cream, essence water, essence liquid, essence oil, sunscreen, sunscreen milk, sunscreen spray, sheet mask, smear mask, mud mask, eye cream, and eye mask.

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