Low-temperature ultrasonic extraction method and application thereof

Abstract

The invention relates to the technical field of daily chemicals, in particular to a low-temperature ultrasonic extraction method and a skin care product. The method comprises the following steps: (1) pretreatment of a to-be-extracted product: removing seeds from the to-be-extracted product, cleaning, crushing, and refrigerating at low temperature for later use; (2) low-temperature ultrasonic extraction: carrying out ultrasonic extraction for 30-40 minutes under the conditions that the temperature is 30-35 DEG C, the ultrasonic power is 20-25 kHz and the power is 300-500 W; (3) separating the extracting solution at 4 DEG C and 4000 rpm for 20 minutes to separate out an upper layer and a lower layer; (4) performing high-shear emulsification on the upper-layer extract by using PEG-40 hydrogenated castor oil to form a stable emulsion; adjusting the pH value of the lower-layer extract to 5.0 by using sodium citrate; and (5) mixing the treated upper-layer extract and lower-layer extract, emulsifying for 3 minutes by using a high-shear emulsifying machine (8000rpm), adding a thickening agent, and continuously stirring. The composition provided by the invention is rich in natural polyphenols, flavonoids, superoxide dismutase (SOD) and vitamin C, and can be used for removing free radicals of skin, reducing oxidative stress and reducing wrinkles.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemicals, in particular to a low-temperature ultrasonic extraction method and application thereof. Background Art

[0002] The extraction of plant active ingredients is a key step in the research of natural products, functional foods, cosmetics, and the development of traditional Chinese medicine preparations. Common extraction techniques include aqueous extraction, alcohol extraction, thermal reflux, microwave-assisted extraction, enzymatic hydrolysis, and ultrasonic-assisted extraction. However, these traditional extraction methods suffer from numerous drawbacks, such as low extraction efficiency, easy destruction of heat-sensitive components, high solvent consumption, and complex post-processing, making them difficult to meet the demand for efficient and gentle extraction of active ingredients.

[0003] In recent years, ultrasound-assisted extraction (UAE) has become a hot topic in research and industrial applications due to its advantages, including short extraction times, low energy consumption, and ease of operation. The cavitation and microfluidic effects produced by ultrasound propagating through a liquid medium can disrupt plant cell structures and increase cell wall permeability, thereby accelerating the extraction of active components from the solvent.

[0004] With the rapid development of natural functional skin care products, sea buckthorn fruit extract has attracted widespread attention due to its "natural origin, high nutritional density, and multi-target skin care", and has become one of the important directions for developing plant-based multifunctional skin care products.

[0005] However, in the existing technology, the application of seabuckthorn fruit extract still faces the following challenges:

[0006] Poor stability: The active ingredients in seabuckthorn, such as vitamin C, flavonoids, and carotenoids, are extremely sensitive to heat, light, and oxygen, and are prone to oxidation, browning, and degradation. This can lead to a decrease in the active ingredient content, color changes, and a short shelf life in skin care products.

[0007] Complex ingredients and large differences in solubility: Sea buckthorn fruit extract contains both water-soluble and fat-soluble components, making it difficult to form a stable dispersion system in traditional emulsions or aqueous solutions, affecting the penetration, absorption and bioavailability of the active ingredients;

[0008] Poor sensory experience: Sea buckthorn raw materials often have a strong natural smell and color, and using them in formulas may cause consumers to have a lower acceptance of the smell and color;

[0009] The preparation process lacks standardization: Existing sea buckthorn extraction methods mostly rely on high-temperature water extraction or alcohol extraction, which can easily lead to thermal degradation of some active ingredients, low extraction rate and poor product batch stability.

[0010] Therefore, there is an urgent need to develop a method for preparing sea buckthorn fruit extract that is mild, efficient, retains complete ingredients, has both antioxidant and skin-friendly properties, and explore its highly stable application form in skin care products to solve the problems of insufficient stability and efficacy expression in existing products. Summary of the Invention

[0011] In order to solve the above technical problems, the present invention provides a low-temperature ultrasonic extraction method, the method steps are as follows:

[0012] (1) Pretreatment of the extract: remove the seeds, clean, crush, and store at low temperature for later use;

[0013] (2) Low-temperature ultrasonic extraction: ultrasonic extraction at 30-35°C, ultrasonic power 20-25kHz, 300-500W for 30-40 minutes;

[0014] (3) Separate the extract at 4°C and 4000 rpm for 20 minutes to separate the upper and lower layers;

[0015] (4) The upper layer extract was emulsified with PEG-40 hydrogenated castor oil under high shear to form a stable emulsion; the lower layer extract was adjusted to pH 5.0 with sodium citrate;

[0016] (5) The treated upper extract and the lower extract were mixed, and then emulsified using a high shear emulsifier (8000 rpm) for 3 minutes, and a thickener was added and the mixture was stirred continuously.

[0017] As an embodiment of the present invention, in step (2), the material-liquid ratio is 1:(5-10).

[0018] As an embodiment of the present invention, in step (2), the material-liquid ratio is 1:8.

[0019] As an embodiment of the present invention, in step (2), the extract is a mixture of water, ethanol and glycerol.

[0020] As an embodiment of the present invention, the volume ratio of water, ethanol and glycerol is 80:15:5.

[0021] As an embodiment of the present invention, in step (2), rosemary is added before ultrasonic extraction.

[0022] As an embodiment of the present invention, the mass ratio of the rosemary to the extract is 1:(100-800).

[0023] As an embodiment of the present invention, the mass ratio of the rosemary to the extract is 1:400.

[0024] An extract is prepared by the preparation method.

[0025] A skin care product, characterized in that it contains the extract.

[0026] By adopting the above technical solution, the present invention has the following beneficial effects:

[0027] The composition provided by this invention is rich in natural polyphenols, flavonoids, superoxide dismutase (SOD), vitamin C, vitamin E, and other nutrients, which can scavenge skin free radicals, reduce oxidative stress, and reduce the formation of wrinkles. The flavonoids and phytosterols in seabuckthorn can significantly inhibit the expression of inflammatory factors (such as IL-6 and TNF-α), effectively reducing inflammatory reactions such as erythema and itching caused by skin irritation, and has excellent skin soothing and calming effects. DETAILED DESCRIPTION

[0028] The present invention will be further explained below with reference to specific embodiments.

[0029] The present invention provides a method for preparing seabuckthorn fruit extract, and the preparation method comprises the following steps:

[0030] (1) Pretreatment of fresh sea buckthorn: remove the seeds, clean, crush and store at low temperature for later use;

[0031] (2) Low-temperature ultrasonic extraction: The sea buckthorn and rosemary powders prepared in step (1) are mixed, and then ultrasonically extracted at a solid-liquid ratio of 1:8 at 30-35°C, an ultrasonic power of 20-25kHz, and 300-500W for 30-40 minutes; wherein the extract is a mixture of water, ethanol, and glycerol in a mass ratio of 80:15:5. The mass ratio of rosemary to sea buckthorn is 1:400.

[0032] (3) Filter the product of step (2), and separate the resulting extract at 4°C and 4000 rpm for 20 minutes to separate the upper and lower layers;

[0033] (4) The upper layer extract was emulsified with 3 wt% PEG-40 hydrogenated castor oil under high shear to form a stable emulsion; the lower layer extract was adjusted to pH 5.0 with sodium citrate;

[0034] (5) The treated upper extract and the lower extract were mixed, and then emulsified for 3 minutes using a high shear emulsifier (8000 rpm), and a thickener (xanthan gum 0.05 wt%) was added and stirred continuously.

[0035] The present invention also provides a seabuckthorn fruit extract prepared by the preparation method.

[0036] The present invention also provides a skin care product, the raw materials for its preparation include:

[0037] Component A:

[0038] 63.68 parts of water;

[0039] 20.0 parts of tea water;

[0040] 5.0 parts of butanediol;

[0041] 0.3 parts of ammonium acryloyldimethyltaurate / VP copolymer;

[0042] 0.3 parts of sodium hyaluronate;

[0043] 0.02 parts of EDTA-2Na;

[0044] Component B:

[0045] 5.0 parts of sea buckthorn fruit extract

[0046] 2.0 parts of Astragalus extract

[0047] 1.5 parts niacinamide

[0048] 1.0 part panthenol

[0049] Component C:

[0050] 0.7 parts of 1,2-hexanediol;

[0051] 0.5 parts of p-hydroxyacetophenone.

[0052] The present invention also provides a method for preparing the skin care product, which comprises the following steps:

[0053] Stir phase A evenly, raise the temperature to 60°C, then heat to 85°C, keep warm for 15 minutes and then cool down; cool down to 50-55°C, add phase B, stir well, then add phase C and stir well, continue to cool down; cool down to 40-45°C, take samples for testing, and filter out the material if it is qualified.

[0054] The present invention provides a multi-solvent synergistic extraction method: a water, low-concentration ethanol, and glycerol composite system that is compatible with hydrophilic and lipophilic components, and simultaneously adds the two components to a biphasic stabilizer; the entire process is treated at low temperature, and pH is controlled externally to prepare an extract with stable performance. At the same time, after adopting biphasic treatment, the obtained efficacy is also more stable and effective.

[0055] Example 1

[0056] This embodiment provides a method for preparing seabuckthorn fruit extract, and the preparation method comprises the following steps:

[0057] (1) Pretreatment of fresh sea buckthorn: remove the seeds, clean, crush and store at low temperature for later use;

[0058] (2) Low-temperature ultrasonic extraction: The sea buckthorn and rosemary powders prepared in step (1) were mixed, and then ultrasonically extracted at a solid-liquid ratio of 1:8 at 30-35°C, an ultrasonic power of 20-25kHz, and 300-500W for 40 minutes; wherein the extract was a mixture of water, ethanol, and glycerol in a mass ratio of 80:15:5. The mass ratio of rosemary to sea buckthorn was 1:400.

[0059] (3) Filter the product of step (2), and separate the resulting extract at 4°C and 4000 rpm for 20 minutes to separate the upper and lower layers;

[0060] (4) The upper layer extract was added with 3 wt% PEG-40 hydrogenated castor oil and high shear emulsified to form a stable emulsion; the lower layer extract was adjusted to pH 5.0 with sodium citrate;

[0061] (5) The treated upper extract and the lower extract were mixed, and then emulsified for 3 minutes using a high shear emulsifier (8000 rpm), and 0.05 wt% xanthan gum was added and continued to stir.

[0062] This embodiment also provides a seabuckthorn fruit extract, which is prepared by the preparation method.

[0063] This embodiment also provides a skin care product, the raw materials for its preparation include:

[0064] Component A: 63.68 parts water; 20.0 parts tea water; 5.0 parts butylene glycol; 0.3 parts ammonium acryloyldimethyltaurate / VP copolymer; 0.3 parts sodium hyaluronate; 0.02 parts EDTA-2Na;

[0065] Component B: 5.0 parts of sea buckthorn fruit extract; 2.0 parts of astragalus extract; 1.5 parts of niacinamide; 1.0 parts of panthenol;

[0066] Component C: 0.7 parts of 1,2-hexanediol; 0.5 parts of p-hydroxyacetophenone.

[0067] This embodiment also provides a method for preparing the skin care product, which comprises the following steps: stirring phase A evenly, heating to 60° C., then heating to 85° C., keeping the temperature for 15 minutes, and then cooling; cooling to 50-55° C., adding phase B, stirring thoroughly, then adding phase C, stirring thoroughly, and continuing to cool; cooling to 40-45° C., sampling and testing, and filtering out the material after passing the test.

[0068] Example 2

[0069] The difference between this embodiment and embodiment 1 is that this embodiment provides a method for preparing seabuckthorn fruit extract, and the steps of the preparation method are as follows:

[0070] (1) Pretreatment of fresh sea buckthorn: remove the seeds, clean, crush and store at low temperature for later use;

[0071] (2) Low-temperature ultrasonic extraction: The sea buckthorn prepared in step (1) is subjected to ultrasonic extraction at 30-35°C, an ultrasonic power of 20-25kHz, and 300-500W for 40 minutes at a material-liquid ratio of 1:8; wherein the extract is a mixture of water, ethanol, and glycerol at a mass ratio of 80:15:5.

[0072] (3) Filter the product of step (2), and separate the resulting extract at 4°C and 4000 rpm for 20 minutes to separate the upper and lower layers;

[0073] (4) The upper layer extract was added with 3 wt% PEG-40 hydrogenated castor oil and high shear emulsified to form a stable emulsion; the lower layer extract was adjusted to pH 5.0 with sodium citrate;

[0074] (5) The treated upper extract and the lower extract were mixed, and then emulsified using a high shear emulsifier (8000 rpm) for 3 minutes, and 0.05 wt% xanthan gum was added and continued to stir.

[0075] The rest is the same as Example 1.

[0076] Example 3

[0077] The difference between this embodiment and embodiment 1 is that this embodiment provides a method for preparing seabuckthorn fruit extract, and the steps of the preparation method are as follows:

[0078] (1) Pretreatment of fresh sea buckthorn: remove the seeds, clean, crush and store at low temperature for later use;

[0079] (2) Low-temperature ultrasonic extraction: The sea buckthorn and rosemary powders prepared in step (1) were mixed, and then ultrasonically extracted at a solid-liquid ratio of 1:8 at 30-35°C, an ultrasonic power of 20-25kHz, and 300-500W for 40 minutes; wherein the extract was a mixture of water, ethanol, and glycerol in a mass ratio of 80:15:5. The mass ratio of rosemary to sea buckthorn was 1:400.

[0080] (3) Filtering the product of step (2) to obtain the extract is the sea buckthorn fruit extract.

[0081] The rest is the same as Example 1.

[0082] Example 4

[0083] The difference between this embodiment and embodiment 1 is that this embodiment provides a method for preparing seabuckthorn fruit extract, and the steps of the preparation method are as follows:

[0084] (1) Pretreatment of fresh sea buckthorn: remove the seeds, clean, crush and store at low temperature for later use;

[0085] (2) Low-temperature ultrasonic extraction: The sea buckthorn and rosemary powders prepared in step (1) were mixed, and then ultrasonically extracted at a solid-liquid ratio of 1:8 at 30-35°C, an ultrasonic power of 20-25kHz, and 300-500W for 40 minutes; wherein the extract was a mixture of water, ethanol, and glycerol in a mass ratio of 80:15:5. The mass ratio of rosemary to sea buckthorn was 1:400.

[0086] (3) Filter the product of step (2), and separate the resulting extract at 4°C and 4000 rpm for 20 minutes to separate the upper and lower layers;

[0087] (4) The upper layer extract was added with 3 wt% PEG-40 hydrogenated castor oil and high shear emulsified to form a stable emulsion; the lower layer extract was adjusted to pH 5.0 with sodium citrate;

[0088] (5) The treated upper layer extract and lower layer extract are separated and named as sea buckthorn fruit upper extract and sea buckthorn fruit lower extract.

[0089] This embodiment also provides a seabuckthorn fruit extract, which is prepared by the preparation method.

[0090] This embodiment also provides a skin care product, the raw materials for its preparation include:

[0091] Component A: 63.68 parts water; 20.0 parts tea water; 5.0 parts butylene glycol; 0.3 parts ammonium acryloyldimethyltaurate / VP copolymer; 0.3 parts sodium hyaluronate; 0.02 parts EDTA-2Na;

[0092] Component B: 5.0 parts of seabuckthorn fruit extract and seabuckthorn fruit extract; 2.0 parts of astragalus extract; 1.5 parts of niacinamide; 1.0 parts of panthenol;

[0093] Component C: 0.7 parts of 1,2-hexanediol; 0.5 parts of p-hydroxyacetophenone.

[0094] This embodiment also provides a method for preparing the skin care product, which comprises the following steps: stirring phase A evenly, heating to 60° C., then heating to 85° C., keeping the temperature for 15 minutes, and then cooling; cooling to 50-55° C., adding phase B, stirring thoroughly, then adding phase C, stirring thoroughly, and continuing to cool; cooling to 40-45° C., sampling and testing, and filtering out the material after passing the test.

[0095] Example 5

[0096] The difference between this embodiment and embodiment 1 is that this embodiment provides a method for preparing seabuckthorn fruit extract, and the steps of the preparation method are as follows:

[0097] (1) Pretreatment of fresh sea buckthorn: remove the seeds, clean, crush and store at low temperature for later use;

[0098] (2) Low-temperature ultrasonic extraction: The sea buckthorn and rosemary powders prepared in step (1) were mixed, and then ultrasonically extracted at a solid-liquid ratio of 1:8 at 30-35°C, an ultrasonic power of 20-25 kHz, and 300-500 W for 40 minutes; wherein the extracting liquid was water. The mass ratio of the rosemary to sea buckthorn was 1:400.

[0099] (3) Filter the product of step (2), and separate the resulting extract at 4°C and 4000 rpm for 20 minutes to separate the upper and lower layers;

[0100] (4) The upper layer extract was added with 3 wt% PEG-40 hydrogenated castor oil and high shear emulsified to form a stable emulsion; the lower layer extract was adjusted to pH 5.0 with sodium citrate;

[0101] (5) The treated upper extract and the lower extract were mixed, and then emulsified using a high shear emulsifier (8000 rpm) for 3 minutes, and 0.05 wt% xanthan gum was added and continued to stir.

[0102] Performance Testing

[0103] Test 1: Transepidermal Water Loss (TEWL) Difference Test

[0104] 1. Subject Information

[0105] Population: Healthy Chinese volunteers aged 25-60 years;

[0106] Gender: 5 males, 35 females; average age: 45.22 years; characteristics: sensitive skin (such as easy redness, stinging, bloodshot eyes, etc.).

[0107] 2. Application area: inner side of forearm;

[0108] Test area: 2cm×2cm; area spacing: at least 1cm; each subject's left and right hands were randomly assigned to the sample group and negative control group, respectively.

[0109] 3. Experimental Procedure

[0110] Preprocessing

[0111] Before the visit: Do not let your arms touch the water;

[0112] After enrollment: clean the test area with dry tissue;

[0113] Rest: Place in an environment of 21±1℃, 50±10%RH for 30 minutes.

[0114] 3.2. Sensitive model induction

[0115] Application induction solution: 0.1% histamine solution;

[0116] Application site: 1 site each in the sample area and control area;

[0117] Application time: 10 minutes;

[0118] Initial detection was performed immediately after removal (Timm).

[0119] 3.3. Sample use: The samples are the products prepared in Examples 1 to 5.

[0120] Application dose: (2.0±0.1)mg / cm 2 ; Coating tool: Latex finger cot spread evenly; Static exposure: Let it stand for 2 hours in a temperature and humidity controlled environment.

[0121] After 1 hour (T1), TEWL was measured using Tewameter and Mexameter, respectively.

[0122] 4. Subjective feelings and satisfaction records

[0123] Self-evaluation time points: 5 minutes, 30 minutes, and 2 hours after sample use; satisfaction questionnaire: fill in 2 hours.

[0124] 4. Main testing indicators

[0125] transepidermal water loss difference (ΔTEWL);

[0126] instrument: TM300 (Courage+Khazaka);

[0127] Calculation formula: ΔTEWL = TEWLTimm - TEWLT1.

[0128] Table 1 Comparison of transepidermal water loss rate before and after

[0129] Example <![CDATA[Average TEWL (Timm) (g / m 2 ·h)]]> <![CDATA[Difference ΔTEWL (g / m 2 ·h)]]> Example 1 25.3±3.1 6.3±1.9 Example 2 25.0±2.9 3.8±2.1 Example 3 24.9±1.9 4.1±2.3 Example 4 25.2±2.0 5.1±2.1 Example 5 25.5±2.3 4.2±1.8 Negative area 25.1±2.7 2.0±2.9

[0130] Subjective comfort feedback (2-hour questionnaire)

[0131] 95% of the subjects said that "the tingling sensation was significantly reduced"; 90% said that "the skin was smoother and moisturized"; and 85% were satisfied or very satisfied with the soothing effect of the product.

[0132] Test 2: By storing cosmetic samples at different temperatures, observe the changes in their stability over a certain period of time and predict their quality performance under normal storage or extreme transportation conditions.

[0133] Sample type: Skin care products of Examples 1 to 5 of the present invention

[0134] Packaging material requirements: Use HDPE test bottles, the final packaging material to ensure good sealing; set no less than 3 bottles of samples for each temperature group.

[0135] Table 2 Temperature condition settings

[0136]

[0137] Table 3 Test results of test 2

[0138]

[0139] Test 3: Hyaluronidase inhibition rate

[0140] Table 4 Materials and reagents

[0141]

[0142]

[0143] Reagent preparation

[0144] Experimental samples (Examples 1 to 5): diluted with purified water to a final concentration of 0.2%.

[0145] Positive control: dipotassium glycyrrhizate (purity ≥ 98%) was selected and prepared into a solution with a mass concentration of 3% using pure water.

[0146] Negative control: Use purified water.

[0147] Experimental design

[0148] The following three groups of experiments were set up, with three parallel samples in each group:

[0149] Example group (experimental sample)

[0150] Positive control group (dipotassium glycyrrhizate)

[0151] Negative control group (pure water)

[0152] Operation steps: Add the corresponding reagent solutions to the reaction system according to the experimental design and mix well; let the reaction stand at room temperature for 30 minutes; after the reaction is completed, add the color developer, continue the reaction and measure the absorbance (OD) value using a UV spectrophotometer at a wavelength of 528nm.

[0153] The hyaluronidase inhibition rate was calculated according to the following formula: Hyaluronidase inhibition rate (%) = (1-(CD) / (AB))×100.

[0154] in:

[0155] A: absorbance of the reaction solution without sample (enzyme + substrate, blank control);

[0156] B: absorbance of the reaction solution without sample and enzyme (blank);

[0157] C: absorbance of the reaction solution containing sample and enzyme;

[0158] D: Absorbance of the reaction solution containing sample but no enzyme.

[0159] Table 5 Test results

[0160] name Hyaluronidase inhibition rate (%) Significant conclusions Example 1 43.26±1.02 P<0.01, significant Example 2 19.15±0.87 P<0.05, significant Example 3 24.21±1.15 P<0.01, significant Example 4 35.74±0.94 P<0.01, significant Example 5 27.92±1.03 P<0.01, significant Negative control -4.965±0.259 / Positive control 51.239±1.314 P<0.01, significant

[0161] As can be seen from Table 5, the hyaluronidase inhibition rate of Example 1 reached 42.38%, close to the activity level of the positive control (dipotassium glycyrrhizate, 51.24%); the inhibition rate of Example 4 (upper and lower layer separation treatment) was 35.64%, which was second best; Example 2 without rosemary powder performed the weakest (18.95%), indicating that the components in rosemary may participate in synergistic inhibition; all examples achieved statistically significant differences compared with the negative control (P<0.05 or P<0.01), indicating that the sea buckthorn fruit extract has clear inhibitory activity against hyaluronidase; the inhibition rate results show that the preparation process is of great significance for the retention of active ingredients, and it is recommended that the process of Example 1 be preferred for product development.

[0162] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A low-temperature ultrasonic extraction method, characterized in that: The method steps are as follows: (1) Pretreatment of the extract: remove the seeds, clean, crush, and store at low temperature for later use; (2) Low-temperature ultrasonic extraction: ultrasonic extraction at 30-35°C, ultrasonic power 20-25kHz, 300-500W for 30-40 minutes; (3) Separate the extract at 4°C and 4000 rpm for 20 minutes to separate the upper and lower layers; (4) The upper layer extract was emulsified with PEG-40 hydrogenated castor oil under high shear to form a stable emulsion; the lower layer extract was adjusted to pH 5.0 with sodium citrate; (5) The treated upper extract and the lower extract were mixed, and then emulsified using a high shear emulsifier (8000 rpm) for 3 minutes, and a thickener was added and the mixture was stirred continuously.

2. A low-temperature ultrasonic extraction method according to claim 1, characterized in that: In the step (2), the material-liquid ratio is 1:(5-10).

3. A low-temperature ultrasonic extraction method according to claim 2, characterized in that: In the step (2), the material-liquid ratio is 1:

8.

4. A low-temperature ultrasonic extraction method according to claim 1, characterized in that: In the step (2), the extract is a mixture of water, ethanol and glycerol.

5. A low-temperature ultrasonic extraction method according to claim 4, characterized in that: The volume ratio of water, ethanol and glycerol is 80:15:

5.

6. A low-temperature ultrasonic extraction method according to claim 1, characterized in that: In the step (2), rosemary is added before ultrasonic extraction.

7. A low-temperature ultrasonic extraction method according to claim 1, characterized in that: The mass ratio of the rosemary to the extract is 1:(100-800).

8. A low-temperature ultrasonic extraction method according to claim 7, characterized in that: The mass ratio of the rosemary to the extract is 1:

400.

9. An extract, characterized in that The invention is prepared by the preparation method according to claims 1 to 7.

10. A skin care product, characterized in that: Contains the extract according to claim 9.

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