Traditional Chinese medicine effective part composition for relieving inflammatory aging, traditional Chinese medicine preparation and application

Abstract

The invention relates to the technical field of traditional Chinese medicines, and provides a traditional Chinese medicine effective part composition for relieving inflammatory aging, a traditional Chinese medicine preparation and application. Comprising 15 to 40 parts of cod polypeptide extract, 15 to 30 parts of oyster polysaccharide extract, 15 to 25 parts of eucommia male flower total terpene extract, 15 to 35 parts of deer blood polypeptide extract, 25 to 50 parts of ginsenoside extract, 10 to 20 parts of grape seed oil VE extract, 20 to 40 parts of cordyceps militaris polysaccharide extract, 5 to 15 parts of haematococcus pluvialis carotene extract and 10 to 25 parts of krill oil astaxanthin extract. The traditional Chinese medicine preparation comprises the traditional Chinese medicine effective part composition and pharmaceutically acceptable auxiliary materials. The invention also provides application of the traditional Chinese medicine effective part composition in preparation of anti-aging medicines, health-care products or cosmetics. Inflammatory aging is relieved through mutual cooperation of three mechanisms of anti-oxidation, immunoregulation and anti-inflammation, and the anti-inflammatory and anti-oxidation health-care food has remarkable anti-inflammatory, anti-oxidation, anti-aging and immunity-improving effects.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine, and in particular to a composition of effective parts of traditional Chinese medicine for alleviating inflammatory aging, a traditional Chinese medicine preparation and an application thereof. Background Art

[0002] Common chronic diseases in the elderly caused by aging significantly reduce the health of the elderly population and increase the economic burden on families and society. The aging process is closely linked to the activity of specific cytokines, particularly increases in the proinflammatory cytokines IL-1β (interleukin-1β), IL-6 (interleukin-6), and TNF-α (tumor necrosis factor-α). The overactivity of these cytokines in the body constitutes a phenomenon known as inflammatory aging, which is not only a biological marker of aging but also a key warning sign of functional decline, the prevalence of chronic diseases, and increased mortality. Inflammatory aging refers to the long-term, low-grade proinflammatory state of the body during aging, manifested by elevated levels of proinflammatory cytokines (such as IL-6 and TNF-α) and a gradual decline in anti-inflammatory capacity. This inflammation is not an acute response to infection or injury, but rather a systemic, chronic inflammation caused by an imbalance in the immune system, forming a vicious cycle with the aging process. Inflammatory aging is closely related to diseases or lesions such as Parkinson's disease, acute lateral sclerosis, multiple sclerosis, atherosclerosis, heart disease and macular degeneration, type 2 diabetes, osteoporosis, and insulin resistance. It also increases the incidence and mortality rate, thereby seriously endangering the health of patients and reducing their quality of life.

[0003] Current clinical practice mainly relies on non-steroidal anti-inflammatory drugs or biological agents (such as IL-6 inhibitors). Although they can suppress inflammation in the short term, long-term use can easily lead to side effects such as gastrointestinal damage and immunosuppression, and they are unable to synchronously regulate aging-related pathways. Existing anti-inflammatory and anti-aging Chinese medicines mostly use whole medicinal materials (such as astragalus and angelica), which have complex ingredients and low content of effective substances, making it difficult to accurately regulate the multi-target mechanism of inflammatory aging. For example, a Chinese patent with publication number CN103393821A discloses a Chinese medicine composition for treating chronic pelvic inflammatory disease. Although it improves inflammation through a combination of oral and external use, its medicinal material compatibility (such as knotweed and safflower) is mainly aimed at local infectious inflammation, lacks the ability to regulate systemic inflammatory aging, and does not integrate multi-dimensional active substances such as immune regulation and cell repair, resulting in limited overall efficacy. Summary of the Invention

[0004] The purpose of the present invention is to provide a composition of effective parts of traditional Chinese medicine, a traditional Chinese medicine preparation and its application for alleviating inflammatory aging. The composition combines marine biological active ingredients with extracts of effective parts of terrestrial traditional Chinese medicine to achieve synergistic enhancement, and alleviates inflammatory aging through the triple mechanism of antioxidant, immunomodulatory and anti-inflammatory, with significant anti-inflammatory, anti-oxidative, anti-aging and immunity-enhancing effects.

[0005] The embodiments of the present invention are achieved through the following technical solutions:

[0006] The present invention provides a composition of effective parts of traditional Chinese medicine for alleviating inflammatory aging, which comprises, by weight:

[0007] 15-40 parts of cod polypeptide extract, 15-30 parts of oyster polysaccharide extract, 15-25 parts of total terpene extract of Eucommia ulmoides male flowers, 15-35 parts of deer blood polypeptide extract, 25-50 parts of ginsenoside extract, 10-20 parts of grape seed oil VE extract, 20-40 parts of Cordyceps militaris polysaccharide extract, 5-15 parts of Haematococcus pluvialis carotenoid extract, and 10-25 parts of krill oil astaxanthin extract.

[0008] The present invention also provides a method for preparing a composition of effective parts of traditional Chinese medicine for alleviating inflammatory aging, which is as follows: the extracts of the above raw materials are combined and concentrated according to a proportion, and auxiliary materials are added to prepare a clinically applicable dosage form.

[0009] The present invention also provides a Chinese medicine preparation for alleviating inflammatory aging, comprising the above-mentioned Chinese medicine effective part composition and pharmaceutically acceptable excipients; wherein the Chinese medicine effective part composition accounts for 40-80% of the total weight of the preparation.

[0010] The present invention also provides an application of the above-mentioned effective part composition of traditional Chinese medicine in the preparation of anti-aging medicine.

[0011] The technical solutions of the embodiments of the present invention have at least the following advantages and beneficial effects:

[0012] The present invention combines marine bioactive ingredients with extracts of effective parts of terrestrial Chinese medicine to achieve synergistic synergy. Through multi-target coverage, cross-dimensional repair, immune homeostasis reconstruction, and synergistic restoration of immune balance, the body's immunity and antioxidant capacity are significantly improved, and inflammatory aging is alleviated. More specifically, the effective part composition of Chinese medicine of the present invention can significantly improve the appearance and brain, liver, heart, and spleen indexes of D-galactose aging model mice, reduce the MDA content in mouse serum and liver, increase SOD and CAT activity, and significantly reduce the levels of pro-inflammatory factors such as TNF-α, IL-6, and IL-1β in mouse serum. The effective ingredient composition of Chinese medicine of the present invention has the advantages of high safety, wide application, and significant anti-inflammatory and antioxidant effects. The composition can be used as an active ingredient to prepare anti-aging drugs, health products, or cosmetics. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the embodiments. It should be understood that the following drawings only illustrate certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying any creative work.

[0014] Figure 1 This figure shows the effect of the effective fraction composition of the traditional Chinese medicine of the present invention on the appearance and morphology of D-galactose aging model mice;

[0015] Figure 2 This figure shows the effect of the effective fraction composition of the traditional Chinese medicine of the present invention on the liver tissue morphology of D-galactose aging model mice;

[0016] Figure 3 This is a graph showing the effect of the effective fraction composition of the Chinese medicine of the present invention on organ indexes in D-galactose aging model mice;

[0017] Figure 4 This is a graph showing the effect of the effective fraction composition of the Chinese medicine of the present invention on the antioxidant level in D-galactose aging model mice;

[0018] Figure 5 This is a graph showing the effect of the effective fraction composition of the traditional Chinese medicine of the present invention on serum inflammatory factors in D-galactose aging model mice;

[0019] Figure 6 This figure shows the effect of the effective fraction composition of the traditional Chinese medicine of the present invention on the survival period of D-galactose aging model mice. DETAILED DESCRIPTION

[0020] To make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention are described clearly and completely below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer are used. Where the manufacturer of the reagents or instruments is not specified, all are conventional products that can be purchased commercially.

[0021] The following is a detailed description of a composition of effective parts of traditional Chinese medicine for alleviating inflammatory aging, a traditional Chinese medicine preparation and its application provided in the embodiments of the present invention.

[0022] A composition of effective parts of traditional Chinese medicine for alleviating inflammatory aging, comprising, by weight:

[0023] 15-40 parts of cod polypeptide extract, 15-30 parts of oyster polysaccharide extract, 15-25 parts of total terpene extract of Eucommia ulmoides male flowers, 15-35 parts of deer blood polypeptide extract, 25-50 parts of ginsenoside extract, 10-20 parts of grape seed oil VE extract, 20-40 parts of Cordyceps militaris polysaccharide extract, 5-15 parts of Haematococcus pluvialis carotenoid extract, and 10-25 parts of krill oil astaxanthin extract.

[0024] Preferably, the composition comprises, by weight: 20-35 parts of cod polypeptide extract, 20-25 parts of oyster polysaccharide extract, 15-20 parts of total terpene extract of Eucommia ulmoides male flowers, 20-30 parts of deer blood polypeptide extract, 35-45 parts of ginsenoside extract, 10-15 parts of grape seed oil VE extract, 25-30 parts of Cordyceps militaris polysaccharide extract, 10-15 parts of Haematococcus pluvialis carotenoid extract, and 18-25 parts of krill oil astaxanthin extract.

[0025] More preferably, the composition comprises, by weight: 30 parts of cod polypeptide extract, 20 parts of oyster polysaccharide extract, 18 parts of total terpene extract of Eucommia ulmoides male flowers, 25 parts of deer blood polypeptide extract, 40 parts of ginsenoside extract, 15 parts of grape seed oil VE extract, 30 parts of Cordyceps militaris polysaccharide extract, 10 parts of Haematococcus pluvialis carotenoid extract, and 20 parts of krill oil astaxanthin extract.

[0026] This invention is the first to scientifically combine extracts from the effective parts of nine traditional Chinese medicines with clear targets for synergistic synergy. Through multi-target coverage, cross-dimensional repair, and immune homeostasis reconstruction, the synergistic restoration of immune balance significantly improves the body's immunity and antioxidant capacity, alleviating inflammatory aging. More specifically, the mechanism by which the effective part composition of traditional Chinese medicine alleviates inflammatory aging is mainly manifested as follows:

[0027] Peptide ingredients synergistically resist oxidation and repair tissues: cod fish peptides can scavenge free radicals and delay skin aging, while deer blood peptides can activate the AMPK signaling pathway to enhance cell autophagy, eliminate senescent cells, promote immune regulation, and reduce the level of inflammatory factors.

[0028] Polysaccharide components regulate oxidative stress and immune function: oyster polysaccharides can scavenge superoxide anion free radicals, inhibit lipid peroxidation, and enhance the cellular antioxidant defense system; Cordyceps militaris polysaccharides can upregulate the expression of antioxidant enzymes (such as CAT, GSH-Px) and reduce mitochondrial oxidative damage; total terpenes from Eucommia ulmoides male flowers can reduce inflammatory responses; ginsenoside extracts are mainly composed of Rg3 and Rh2, which can regulate immune balance and inhibit TNF-α expression, improve mitochondrial function, and delay cell aging; grape seed oil VE can block lipid peroxidation chain reactions; Haematococcus pluvialis astaxanthin and krill oil astaxanthin can synergistically penetrate the blood-brain barrier, inhibit DNA oxidative damage, and synergistically enhance the antioxidant network with grape seed oil VE.

[0029] Specifically, the preparation method of the extract of each effective part of traditional Chinese medicine is as follows:

[0030] It should be noted that before extraction, the male flowers of Eucommia ulmoides, ginseng, Cordyceps militaris, and Haematococcus pluvialis must first be dried and crushed, and passed through a 20-40 mesh sieve;

[0031] The preparation method of the cod polypeptide extract is as follows: thawing the cod maw at 2-6°C, washing it, cutting it into small pieces, soaking it in 10-15 times the volume of 0.1 mol / L sodium hydroxide solution, stirring it for 48 hours, changing the alkaline solution every 6 hours, washing it with water until it is neutral, adding 10 times the volume of 10% isopropyl alcohol, degreasing it for 24 hours, changing the solution every 6 hours, and washing the sample with water to obtain the pretreated cod maw;

[0032] The pretreated cod maw was added to 10 times the volume of water, extracted at 100-110°C for 2-5 hours, and then cooled to room temperature for homogenization. After adjusting the pH to 7.0, a composite protease (CAS No. 9014-01-1, Product No.: C8800, brand SOLARBIO / Solebao) was added at an enzyme addition amount of 100U / mL. The enzyme was hydrolyzed at 50-60°C for 2-5 hours. After the enzymatic hydrolysis, the enzyme was heated to 100-120°C for 10 minutes to inactivate the enzyme. 10g / L powdered activated carbon was added, decolorized for 1 hour, and centrifuged for 20-30 minutes. The supernatant was subjected to a 200Da nanofiltration membrane desalination cycle for 3-5 times under a pressure of 1-2MPa. The treated liquid was collected and concentrated to obtain the cod polypeptide extract.

[0033] More specifically, the content of polypeptides in the cod polypeptide extract is not less than 40%.

[0034] Furthermore, the preparation method of the oyster polysaccharide extract is as follows: fresh oysters are washed and drained, 10-15 times the amount of water is added and boiled for 5-15 minutes, cooled to room temperature, crushed and homogenized with a colloid mill, the pH is adjusted to 8.5-9.5, 3-5% alkaline protease is added, and enzymatic hydrolysis is carried out at 40-60°C for 1-3 hours. After the enzymatic hydrolysis is completed, the enzyme is inactivated by heating to 90-100°C for 10 minutes, centrifuged for 5-10 minutes, the supernatant is taken, 3 times the volume of anhydrous ethanol is added, and the mixture is precipitated at 2-6°C overnight and centrifuged for 1-3 minutes. n, collect the precipitate, and freeze-dry to obtain oyster crude glycogen; then, DEAE-52 cellulose anion exchange column chromatography was used to grade and purify the crude oyster glycogen, eluting it with distilled water, 0.1mol / L, 0.2mol / L, 0.3mol / L, 0.4mol / L and 0.5mol / L NaCl solutions in sequence, and then using Sepharose 2B gel column chromatography for separation and purification, and 0.15mol / L NaCl was used for linear elution to obtain oyster polysaccharide extract.

[0035] More specifically, the oyster polysaccharide extract comprises glycogen and heteropolysaccharides, and the polysaccharide content of the extract is 40% to 70%, wherein the glycogen content is not less than 45% of the polysaccharide.

[0036] Furthermore, the preparation method of the total terpene extract of Eucommia ulmoides male flowers is as follows: adding 25 times the amount of 50-70% ethanol solution to the male flowers of Eucommia ulmoides, extracting 3-5 times at 70-90°C, each time for 1-2 hours, combining the extracts, and concentrating under reduced pressure until there is no alcohol taste; then adsorbing through AB-8 type macroporous adsorption resin, eluting with 50-70% ethanol eluent, collecting and concentrating to obtain the total terpene extract of Eucommia ulmoides male flowers.

[0037] More specifically, the total terpene extract of Eucommia ulmoides male flowers includes geniposide and at least one of Aucubin, geniposidic acid, eucommiol, and 1-deoxyeucommiol. The total terpene content in the extract is 16% to 48%, wherein the geniposide content is not less than 35% of the total terpenes.

[0038] Furthermore, the preparation method of the deer blood polypeptide extract is as follows: freeze-drying deer blood plasma to prepare freeze-dried powder, adding the freeze-dried powder to 5 times the amount of water, and ultrasonically treating it at 20-30kHz for 10-20 minutes, adjusting the pH to 7-8, adding 5-6% of a mixed enzyme solution of trypsin and neutral protease, and performing enzymatic hydrolysis at 50-60°C for 2-5 hours. After the enzymatic hydrolysis is completed, heating to 90-100°C to inactivate the enzyme for 10-20 minutes, cooling to room temperature, centrifuging for 10-20 minutes, collecting and concentrating the supernatant, and thus obtaining the deer blood polypeptide extract.

[0039] More specifically, the content of polypeptides in the deer blood polypeptide extract is no less than 60%.

[0040] Furthermore, the preparation method of the ginseng total saponin extract is as follows: adding ginseng to 8 to 10 times the amount of 70%-80% ethanol, extracting at 60 to 85°C for 2 to 5 times, each time for 2 to 5 hours, combining the extracts, filtering and concentrating under reduced pressure, adding the concentrate to 2 to 3 times the amount of water, stirring for 30 to 60 minutes, standing overnight, centrifuging to obtain the supernatant, passing through AB-8 macroporous adsorption resin, first washing with water, then eluting with 10%, 30%, and 60% to 70% ethanol, collecting the 60% to 70% ethanol eluate, and concentrating to obtain the ginseng total saponin extract.

[0041] More specifically, the ginsenoside extract comprises Ginsenoside Rg3 and at least one of Ginsenoside Rh2, Ginsenoside Rb1 and Ginsenoside Ra1, wherein the content of total saponins in the extract is 40% to 80%, wherein the content of Ginsenoside Rg3 is not less than 40% of the total saponins.

[0042] Furthermore, the preparation method of the grape seed oil VE extract comprises: deodorizing grape seed crude oil to obtain a distillate, adding 3-7 times the amount of acidic methanol to the distillate, stirring and mixing, saponifying for 1-1.5 hours, adding NaOH for neutralization, and then standing to separate the layers, taking the oil phase and cold-crystallizing at a low temperature of -4-5°C, and molecularly distilling the concentrate after distillation of the filtrate at 220-240°C and 0.5-0.7MPa, and concentrating to obtain the grape seed oil VE extract.

[0043] More specifically, the grape seed oil VE extract includes at least one of γD-γ-Tocotrienol and α-Tocopherol, or α-Tocotrienol and γ-Tocopherol, and the VE content of the extract is 30% to 60%, wherein the content of D-γ-Tocotrienol is not less than 40% of the VE.

[0044] Furthermore, the preparation method of the Cordyceps militaris polysaccharide extract is as follows: add Cordyceps militaris to 15 to 20 times the amount of water, extract at 70 to 80° C. for 2 to 5 times, each time for 1 to 3 hours, combine the extracts, filter, and concentrate under reduced pressure to obtain a concentrated solution, then add 2 to 5 times the amount of ethanol to the concentrated solution and stir, let it stand overnight, centrifuge to obtain a precipitate, add water to dissolve it, pass it through a microfiltration membrane and a nanofiltration membrane, collect the filtrate and concentrate it to obtain the Cordyceps militaris polysaccharide extract.

[0045] More specifically, the Cordyceps militaris polysaccharide extract is composed of mannose, galactose and galacturonic acid, and the polysaccharide content is 20% to 50%, wherein the mannose content is not less than 30% of the polysaccharide, and the galacturonic acid content is not less than 15% of the polysaccharide;

[0046] Furthermore, the preparation method of the Haematococcus pluvialis carotenoid extract is as follows: adding 30-50 times the amount of 0.5-1 mg / mL cellulase solution to Haematococcus pluvialis powder, adjusting the pH to 4.0, breaking the algae wall at 50-60°C for 30 minutes, centrifuging for 5 minutes, discarding the supernatant, adding acetone, extracting for 30-60 minutes, centrifuging again, discarding the lower precipitate, selecting n-hexane-acetonitrile-methanol (4:1:2, v / v / v) as the primary solvent system and n-hexane-methanol (2:1, v / v) as the secondary solvent system, and purifying and collecting by high-speed countercurrent chromatography to obtain the Haematococcus pluvialis carotenoid extract.

[0047] More specifically, the carotenoid extract of Haematococcus pluvialis includes astaxanthin linolenic acid monoester and at least one of β-carotene, canthaxanthin, astaxanthin hexadecadienoic acid monoester, astaxanthin linoleic acid monoester, and astaxanthin palmitic acid-docosadienoic acid diester. The total carotenoid content in the extract is 15% to 40%, of which the content of astaxanthin linolenic acid monoester is not less than 25% of the carotenoids.

[0048] Furthermore, the preparation method of the krill oil astaxanthin extract is as follows: after thawing, the krill is dried to a moisture content of less than 10%, crushed to 20-40 mesh, 3-7 times the amount of n-hexane-acetone solution (3:1) is added, and the mixture is stirred and extracted at 4-10°C for 2 hours. After filtering, the filtrate is rotary evaporated to remove the solvent to obtain red shrimp oil, which is dissolved in a small amount of petroleum ether and applied to a silica gel chromatography column. It is eluted with petroleum ether-acetone (4:1) solution at a flow rate of 2-5 mL / min, and the red eluate is collected in sections and concentrated by rotary evaporation to obtain the krill oil astaxanthin extract.

[0049] More specifically, the krill oil astaxanthin extract includes at least one of astaxanthin diester, astaxanthin monoester and free astaxanthin, and the astaxanthin content of the extract is 10% to 40%, wherein the astaxanthin diester content is not less than 30% of the astaxanthin.

[0050] More specifically, the extracts of the above-mentioned effective parts were separated and purified by silica gel column chromatography, dextran gel chromatography, semi-preparative liquid chromatography, column chromatography, thin layer chromatography and other techniques, and their structures were analyzed by NMR, HPLC, MS, GC, IR and other techniques. It was finally determined that the content of polypeptides in the cod polypeptide extract was not less than 43%; the total polysaccharide content of the oyster polysaccharide extract was not less than 75%, of which the glycogen content was not less than 40%, and not less than 45% of the polysaccharides; the total terpene content of the eucommia male flower extract was not less than 41%, of which the geniposide content was not less than 26%, and not less than 35% of the total terpenes; the polypeptide content of the deer blood polypeptide extract was not less than 66%; the VE content of the grape seed oil VE extract was not less than 52%, of which the γ-tocotrienol content was not less than 31%; %, not less than 40% of VE; the content of total saponins in the ginsenoside extract is not less than 63%, of which the content of ginsenoside Rg3 is not less than 33%, not less than 40% of the total saponins content; the content of total polysaccharides in the Cordyceps militaris polysaccharide extract is not less than 36%, of which the content of mannose is not less than 20%, not less than 30% of the polysaccharide content, and the content of galacturonic acid is not less than 13%, not less than 15% of the polysaccharide content; the content of total carotenoids in the Haematococcus pluvialis carotenoid extract is not less than 37%, of which the content of astaxanthin linolenic acid monoester is not less than 36%, not less than 25% of carotenoids; the content of total astaxanthin in the krill oil astaxanthin extract is not less than 37%, of which the content of astaxanthin diester is not less than 18%, not less than 30% of astaxanthin.

[0051] The effective component composition of traditional Chinese medicine provided by the present invention can significantly improve the appearance and morphology of D-galactose aging model mice and the indices of the brain, liver, heart, and spleen, reduce the MDA content in the mouse serum and liver, increase the activity of superoxide dismutase (SOD) and catalase (CAT), and significantly reduce the levels of pro-inflammatory factors such as TNF-α, IL-6, and IL-1β in the mouse serum. The effective component composition of traditional Chinese medicine provided by the present invention has the advantages of high safety, wide application, and significant anti-inflammatory and antioxidant effects. The composition can be used as an active ingredient in the preparation of anti-aging drugs, health products, or cosmetics.

[0052] A method for preparing a composition of effective parts of traditional Chinese medicine for alleviating inflammatory aging is as follows: the extracts of the above raw materials are combined according to a proportion, concentrated, and excipients are added to prepare a clinically applicable dosage form.

[0053] The present invention also provides a Chinese medicine preparation for alleviating inflammatory aging, comprising the above-mentioned Chinese medicine effective part composition and pharmaceutically acceptable excipients; wherein the Chinese medicine effective part composition accounts for 40-80% of the total weight of the preparation.

[0054] Furthermore, the dosage forms of the traditional Chinese medicine preparation include oral preparations and external preparations.

[0055] Furthermore, the oral preparations include tablets, granules, pills, powders, capsules and syrups; the external preparations include aerosols, creams, gels and transdermal patches.

[0056] Furthermore, the excipients include any one or more of excipients, disintegrants, lubricants, binders, fillers, sweeteners, preservatives or flavorings; optionally, they include: starch, sodium carboxymethyl starch, talc, polyethylene glycol, micropowdered silica gel, starch slurry (paste), methyl cellulose, microcrystalline cellulose, magnesium stearate, povidone, powdered sugar, syrup, lactose, sucrose, mannitol, benzoic acid, sodium benzoate, glycerin, menthol, etc.

[0057] The present invention also provides an application of the above-mentioned effective part composition of traditional Chinese medicine in the preparation of anti-aging medicines, health products or cosmetics.

[0058] Furthermore, there is provided a use of the above-mentioned composition of effective parts of traditional Chinese medicine in the preparation of medicines, health products or cosmetics for enhancing body immunity, improving antioxidant capacity and delaying cell aging.

[0059] Furthermore, the present invention also provides a use of the above-mentioned effective part composition of traditional Chinese medicine in the preparation of anti-aging medicines, health products or cosmetics that promote skin health, reduce wrinkles and spots, and improve skin elasticity.

[0060] Example 1

[0061] This embodiment provides a composition of effective parts of traditional Chinese medicine for alleviating inflammatory aging, comprising, by weight: 20 parts of cod fish polypeptide extract, 20 parts of oyster polysaccharide extract, 15 parts of total terpenoid extract of Eucommia ulmoides male flowers, 20 parts of deer blood polypeptide extract, 35 parts of ginsenoside extract, 10 parts of grape seed oil VE extract, 25 parts of Cordyceps militaris polysaccharide extract, 10 parts of Haematococcus pluvialis carotenoid extract, and 18 parts of krill oil astaxanthin extract;

[0062] This embodiment also provides a tablet for alleviating inflammatory aging, and the preparation method thereof is as follows:

[0063] (1) Dried eucommia ulmoides flowers, ginseng, cordyceps militaris, and Haematococcus pluvialis were crushed and passed through a 30-mesh sieve;

[0064] (2) Thaw the cod bladder at 4°C, clean it, cut it into small pieces, soak it in 10 times the volume of 0.1 mol / L sodium hydroxide solution, stir it for 48 hours, change the alkali solution every 6 hours, wash it with water until it is neutral, add 10 times the volume of 10% isopropanol, remove fat for 24 hours, change the solution every 6 hours, wash the sample with water, add 10 times the volume of water to the pretreated cod bladder, extract it at 105°C for 4 hours, cool it to room temperature and homogenize it, adjust the pH to 7.0 and then add 100U / mL of enzyme was added with composite protease (CAS No. 9014-01-1, Product No.: C8800, brand SOLARBIO / Solaribo), and enzymatic hydrolysis was carried out at 55°C for 4 hours. After the enzymatic hydrolysis, the enzyme was inactivated by heating to 100°C for 10 minutes. 10 g / L of powdered activated carbon was added, and the mixture was decolorized for 1 hour and centrifuged for 20 minutes. The supernatant was subjected to cyclic desalting treatment through a 200 Da nanofiltration membrane at a pressure of 1.4 MPa for three times. The treated liquid was collected and concentrated to obtain the cod polypeptide extract;

[0065] (3) Wash and drain the fresh oysters, add 10 times the amount of water and boil for 10 minutes, cool to room temperature, crush and homogenize with a colloid mill, adjust the pH to 9.0, add 4% alkaline protease, and enzymatically hydrolyze at 50°C for 2 hours. After the enzymatic hydrolysis, heat to 95°C to inactivate the enzyme for 10 minutes, centrifuge for 5 minutes, take the supernatant, add 3 times the volume of anhydrous ethanol, precipitate at 4°C overnight, centrifuge for 1 minute, collect the precipitate, and freeze-dry to obtain oyster crude glycogen. The crude oyster glycogen is fractionally purified by DEAE-52 cellulose anion exchange column chromatography, and eluted with distilled water, 0.1 mol / L, 0.2 mol / L, 0.3 mol / L, 0.4 mol / L and 0.5 mol / L NaCl solutions in sequence. Separate and purify using Sepharose 2B gel column chromatography, linear elution with 0.15 mol / L NaCl, collect the eluate, and concentrate to obtain oyster polysaccharide extract;

[0066] (4) Add 25 times the amount of 60% ethanol solution to the male pollen of Eucommia ulmoides and extract it at 80°C for 4 times, each time for 1.5 hours. Combine the extracts and concentrate under reduced pressure until there is no alcohol taste. Adsorb the target component through AB-8 macroporous adsorption resin and elute with 60% ethanol. Collect and concentrate the eluate to obtain the total terpene extract of the male flowers of Eucommia ulmoides.

[0067] (5) Deer blood plasma was freeze-dried into powder, and the freeze-dried powder was added with 5 times the amount of water and subjected to 20 kHz ultrasonic treatment for 15 minutes. After adjusting the pH to 7.5, a mixed enzyme solution of 5.5% trypsin and neutral protease was added and enzymatically hydrolyzed at 50°C for 4 hours. After the enzymatic hydrolysis, the solution was heated to 95°C for 10 minutes to inactivate the enzyme, cooled to room temperature, centrifuged for 15 minutes, and the supernatant was collected and concentrated to obtain the deer blood polypeptide extract;

[0068] (6) Ginseng was extracted twice with 10 times the amount of 80% ethanol at 75°C for 3 hours each time. The combined extracts were filtered and concentrated under reduced pressure. 2.5 times the amount of water was added to the concentrate, stirred for 30 minutes, and allowed to stand overnight. The supernatant was centrifuged and passed through AB-8 macroporous adsorption resin, washed with water, and eluted with 10%, 30%, and 60% ethanol. The 60% ethanol eluate was collected and concentrated to obtain the ginsenoside extract.

[0069] (7) Add 5 times the amount of acidic methanol to the deodorized distillate of grape seed oil, stir and mix, saponify for 1.5 hours, add NaOH to neutralize and let stand for stratification, take the oil phase and cold crystallize at -4°C, the concentrate after distillation of the filtrate is molecularly distilled at 230°C and 0.6MPa to obtain concentrated grape seed oil VE extract;

[0070] (8) Cordyceps militaris powder was extracted with 15 times the amount of water at 80°C for 3 times, each time for 2 hours. The combined extracts were filtered and concentrated under reduced pressure. 4 times the amount of ethanol was added to the concentrate and stirred. The concentrate was allowed to stand overnight. The precipitate was centrifuged and dissolved in water and passed through a microfiltration membrane and a nanofiltration membrane. The filtrate was collected and concentrated to obtain a Cordyceps militaris polysaccharide extract.

[0071] (9) 40 times the amount of 0.6 mg / mL cellulase solution was added to the algae powder of Haematococcus pluvialis, and after adjusting the pH to 4.0, the algae cell wall was broken at 50°C for 30 minutes, centrifuged for 5 minutes, and the supernatant was discarded. Acetone was added and the mixture was extracted for 40 minutes. After centrifugation again, the lower precipitate was discarded, and n-hexane-acetonitrile-methanol (4:1:2, v / v / v) was selected as the primary solvent system and n-hexane-methanol (2:1, v / v) was selected as the secondary solvent system. The target component was purified and collected by high-speed countercurrent chromatography to obtain the carotenoid extract of Haematococcus pluvialis.

[0072] (10) After thawing, Antarctic krill was dried to a water content of less than 10%, crushed to 40 mesh, added with 5 times the amount of n-hexane-acetone solution (3:1), stirred and extracted at 5°C for 2 h, filtered, and the filtrate was rotary evaporated to remove the solvent to obtain red shrimp oil. After the shrimp oil was dissolved, it was loaded onto the sample and eluted with petroleum ether-acetone (4:1) solution at a flow rate of 2 mL / min. The red eluate was collected in sections and concentrated by rotary evaporation to obtain krill oil astaxanthin extract;

[0073] (11) The extracts obtained above were mixed evenly, 20% microcrystalline cellulose was added, stirred evenly, dried under reduced pressure at 60°C until the moisture content was less than 5%, crushed through an 80-mesh sieve to obtain a mixed powder, 2% magnesium stearate was added, mixed evenly, and compressed into tablets using a tablet press.

[0074] Example 2

[0075] The difference between this embodiment and Example 1 is that the tablets of this embodiment include, by weight, 30 parts of cod polypeptide extract, 20 parts of oyster polysaccharide extract, 18 parts of total terpene extract of Eucommia ulmoides male flowers, 25 parts of deer blood polypeptide extract, 40 parts of ginsenoside extract, 15 parts of grape seed oil VE extract, 30 parts of Cordyceps militaris polysaccharide extract, 10 parts of Haematococcus pluvialis carotenoid extract, and 20 parts of krill oil astaxanthin extract.

[0076] Example 3

[0077] The difference between this embodiment and embodiment 1 is that this embodiment is a granule, which comprises, by weight: 30 parts of cod polypeptide extract, 20 parts of oyster polysaccharide extract, 18 parts of total terpenoid extract of Eucommia ulmoides, 25 parts of deer blood polypeptide extract, 40 parts of ginsenoside extract, 15 parts of grape seed oil VE extract, 30 parts of Cordyceps militaris polysaccharide extract, 10 parts of Haematococcus pluvialis carotenoid extract, and 20 parts of krill oil astaxanthin extract;

[0078] The preparation method is as follows: after uniformly mixing the extracts, heat and concentrate, add 5% corn starch, stir thoroughly to make the liquid medicine form a soft material, sieve the soft material to granulate, dry until the moisture content is less than 5%, granulate, and package to obtain granules.

[0079] Comparative Example 1

[0080] The difference between this comparative example and Example 2 is that it does not contain the total terpene extract of Eucommia ulmoides male flowers and the astaxanthin extract of krill oil.

[0081] Comparative Example 2

[0082] The difference between this comparative example and Example 2 is that it does not contain deer blood polypeptide extract, ginsenoside extract and grape seed oil VE extract.

[0083] Experimental Example 1-Determination of the content of effective parts of traditional Chinese medicine

[0084] The extracts of the effective parts obtained in Example 1 were separated and purified by silica gel column chromatography, dextran gel chromatography, semi-preparative liquid chromatography, column chromatography, thin layer chromatography and other techniques, and the structures were analyzed by NMR, HPLC, MS, GC, IR and other techniques, and the following were finally determined:

[0085] The polypeptide content of cod polypeptide extract is 43%; the total polysaccharide content of oyster polysaccharide extract is 75%, of which the glycogen content is 40%, not less than 45% of the polysaccharide; the total terpene content of Eucommia ulmoides male flower extract is 41%, of which the geniposide content is 26%, not less than 35% of the total terpene; the polypeptide content of deer blood polypeptide extract is 66%; the VE content of grape seed oil VE extract is 52%, of which the γ-tocotrienol content is 31%, not less than 40% of the VE; the total saponin content of ginsenoside extract is 63%, of which the content of ginsenoside Rg3 is The content of total saponins is 33%, not less than 40% of the total saponin content; the content of total polysaccharides in the Cordyceps militaris polysaccharide extract is 36%, of which the content of mannose is 20%, not less than 30% of the polysaccharide content, and the content of galacturonic acid is 13%, not less than 15% of the polysaccharide content; the content of total carotenoids in the Haematococcus pluvialis carotenoid extract is 37%, of which the content of astaxanthin linolenic acid monoester is 36%, not less than 25% of the carotenoids; the content of total astaxanthin in the krill oil astaxanthin extract is 37%, of which the content of astaxanthin diester is 18%, not less than 30% of astaxanthin.

[0086] Experimental Example 2 - Therapeutic effect of the effective fraction composition of the Chinese medicine of the present invention on D-galactose-induced aging mice

[0087] 2.1 Model Construction: 48 mice weighing 18-24 g were selected for adaptive feeding for one week and randomly divided into 8 groups, with 6 mice in each group, namely:

[0088] The blank control group, model control group, positive drug control group, low-dose group, medium-dose group and high-dose group of the tablets obtained in Example 1 (hereinafter referred to as low-dose group, medium-dose group and high-dose group), comparative example 1 group and comparative example 2 group; wherein, the dosage of each administration of the low-dose group, medium-dose group and high-dose group was 0.59 g / kg, 1.17 g / kg and 2.34 g / kg respectively;

[0089] Starting from the 8th day, except for the blank control group, the models of rats in other groups were established as follows: each group of mice was intraperitoneally injected with D-galactose (150 mg / kg), and the blank control group was intraperitoneally injected with 0.2 mL of normal saline, once a day for 8 consecutive weeks.

[0090] 2.2 Administration: At the same time as modeling, the low-dose group, the medium-dose group and the high-dose group were gavaged with different doses of the pharmaceutical composition prepared in Example 1 (i.e., 0.59 g / kg, 1.17 g / kg, 2.34 g / kg, with physiological saline solution as a solvent); the comparative example 1 group and the comparative example 2 group were gavaged with 2.34 g / kg of the drugs prepared in comparative examples 1 and 2, respectively, the positive drug group was gavaged with 0.1 g / kg of vitamin C, and the blank control group and the model group were gavaged with 10 mL / kg of physiological saline solution, once a day, for 8 consecutive weeks. During the experiment, the body weight, appearance and hair color, body shape changes, behavioral activities, diet and mental state of the mice in each group were observed and recorded. The results are shown in the figure below. Figure 1 As shown,

[0091] Depend on Figure 1 It can be seen that the mice in the blank control group had a normal diet, shiny fur and good skin elasticity. The mice in the aging model control group induced by D-galactose had dry and dull hair, gradually became thin, moved slowly, had significantly reduced spontaneous activity, reduced food intake, slow reaction, and lack of energy, showing aging characteristics, indicating that the model was successfully established. After gavage with different doses of the effective part composition of the Chinese medicine of the present invention, the appearance and physical signs of the mice in the low-dose group, the medium-dose group and the high-dose group were significantly improved, with normal activity frequency, more agile movement, normal diet, good mental state, and shiny and thick fur; the fur color, mental state and action state of the mice in the positive control group were good, but significantly worse than the fur color, mental state and action state of the mice in the high-dose group, indicating that the high-dose group of the effective part composition of the Chinese medicine of the present invention had a better effect on improving the appearance and behavior of the aging model mice induced by D-galactose than the positive control vitamin C.

[0092] In addition, the body weight results of mice in each group from 1 to 8 weeks are shown in Table 1.

[0093] Table 1 - Mouse body weight (unit: g)

[0094] Blank control group Model control group Positive control group Low-dose group Medium dose group High-dose group Week 1 32.42±1.45 32.68±1.63 32.91±2.18 30.29±1.40 34.00±1.62 32.49±2.28 Week 2 40.06±2.43 38.63±2.16 37.99±2.21 36.38±1.82 38.72±2.03 37.28±3.14 Week 3 44.25±2.68 40.50±1.59 41.43±2.28 40.55±2.34 41.61±3.14 41.91±3.12 Week 4 46.69±2.92 42.86±2.83 42.97±2.29 42.11±2.29 43.00±2.97 43.84±2.77 Week 5 47.84±3.14 43.67±2.53 45.61±2.25 44.29±2.53 45.51±2.79 45.11±4.50 Week 6 48.94±3.44 44.47±3.03 46.56±2.28 44.04±1.88 45.93±3.39 45.72±2.85 Week 7 49.12±3.79 45.05±3.26 48.00±1.76 44.82±1.97 46.38±2.66 47.49±3.52 Week 8 49.75±3.62 45.17±2.62 50.04±2.08 45.93±1.83 47.81±3.03 48.41±2.86

[0095] As shown in Table 1, there was no significant difference in the weight of mice in each group before the experiment, and the weight of mice in each group increased slowly. The weight gain of mice in the model control group was significantly slower than that in other groups, indicating that the model was successfully established. The weight of mice in the high and medium dose groups of the effective fraction composition of the Chinese medicine of the present invention were not much different from those in the blank group and the positive control group. This indicates that the effective fraction composition of the Chinese medicine of the present invention has a good effect on improving the appearance and behavior of aging model mice induced by D-galactose.

[0096] Experimental Example 3 - Effect of the effective fraction composition of traditional Chinese medicine of the present invention on the liver tissue morphology of mice

[0097] Experimental method: The liver tissues of mice in each group were fixed in 4% paraformaldehyde solution, dehydrated, embedded in paraffin, and sliced. Then, they were stained with hematoxylin-eosin (HE). The morphological changes were observed under an optical microscope. The experimental results are shown in Table 1. Figure 2 As shown,

[0098] Depend on Figure 2 It can be seen that the liver tissue morphology of the blank control group mice was intact, with clear structure, uniform cytoplasm, and normal nuclear morphology, while the liver lobule structure of the model control group mice was blurred, partially accompanied by inflammatory cell infiltration, pyknosis of the cell nuclei, and a large number of necrotic cells. Compared with the model group, the low-dose group and the comparative example 1-2 group had some fat vacuoles and inflammatory cell infiltration, and unclear hepatic cords; the medium-dose group and the high-dose group had basically similar tissue states to the positive control group, with normal hepatic lobule morphology, clearer hepatic cords, less inflammatory cell infiltration, normal nuclear morphology, and fewer necrotic cells. This shows that the pharmaceutical composition of the present invention can improve the phenomenon of liver tissue morphological destruction in D-galactose-induced aging model mice. The improvement effect of the medium and high-dose groups is not much different from that of the positive control group, and can achieve the pharmacological effect of vitamin C.

[0099] Experimental Example 4 - Effects of the Composition of Effective Fractions of Traditional Chinese Medicine of the Present Invention on Organ Indexes in Mice

[0100] Experimental Method: Mice were sacrificed by vertebral dislocation, and the brain, liver, heart, and spleen were removed separately. The surrounding adhesions and connective tissue were carefully removed, and the organs were placed on filter paper to absorb moisture. The organs were accurately weighed using an analytical balance, and the organ index was calculated: organ index (mg / g) = organ weight (mg) / mouse body weight (g).

[0101] The organ index reflects the structural changes of the animal's organs and the overall nutritional status, and is an important indicator for judging whether the mouse is aging. As the body ages, the functions of various tissues and organs gradually decline and the organs atrophy. The organ indexes of the liver, spleen, heart and brain tissues of each group of mice are as follows: Figure 3 As shown, Figure 3 AD are the results of brain index, liver index, heart index and spleen index of each group of mice respectively; Figure 3 The results showed that compared with the blank control group, the organ indexes of the brain, liver, spleen and heart of the mice in the D-galactose aging model group were significantly reduced (P < 0.05), indicating that the model was relatively successful.

[0102] like Figure 3 A. Compared with the aging model control group, the brain index of the aging mice in the positive control group, low-dose group, medium-dose group and high-dose group increased significantly (P < 0.05), while the brain index of the aging mice in the control group 1-2 increased, but there was no significant difference (P > 0.05); Figure 3B. Compared with the aging model control group, the liver index of the aging mice in the positive control group and the high-dose group increased significantly (P < 0.05), while the liver index of the aging mice in the low-dose group, the medium-dose group, and the control group 1-2 increased, but there was no significant difference (P > 0.05); Figure 3 C. Compared with the aging model control group, the cardiac index of the aging mice in the positive control group, the medium-dose group, the high-dose group, and the comparative example 1 group increased significantly (P < 0.05), while the cardiac index of the aging mice in the low-dose group and the comparative example 2 group increased, but there was no significant difference (P > 0.05); Figure 3 D. Compared with the aging model control group, the spleen index of the aging mice in the positive control group, low-dose group, medium-dose group, and high-dose group increased significantly (P < 0.05). The spleen index of the aging mice in the control group 1-2 increased, but there was no significant difference (P > 0.05). In summary, the low-dose group, medium-dose group, and high-dose group of the effective fraction composition of the present invention can significantly increase the organ indexes of the liver, spleen, heart, and brain tissues, improve the atrophy of various organ tissues in aging mice, and have excellent anti-aging effects.

[0103] Experimental Example 5-Effect of the Composition of Effective Parts of Traditional Chinese Medicine of the Present Invention on Oxidative Stress Indicators in Mice

[0104] Experimental Methods: Brain and liver tissues from each group of mice were accurately weighed, added to 9 times the volume of 4°C normal saline, and homogenized in an ice-water bath. The homogenates were centrifuged at 4°C, 2500 rpm, for 10 minutes, and the supernatant was collected for later use. Following the instructions in each kit, SOD and CAT activities, as well as MDA content, were measured in the brain and liver tissues of the mice.

[0105] The results of SOD, CAT activities and MDA content determination in brain and liver tissues of mice in each group are as follows Figure 4 As shown, Figure 4 AF are the results of liver SOD activity, brain tissue SOD activity, liver MDA activity, brain tissue MDA activity, liver CAT activity, and brain tissue CAT activity in each group of mice, respectively; Figure 4 The results showed that compared with the normal control group, the SOD and CAT activities in the liver and brain tissues of the D-galactose aging model group, i.e. the model control group, were significantly reduced, and the MDA content was significantly increased (P < 0.05), indicating that the aging model was successfully established.

[0106] like Figure 4As shown in A and B, compared with the model control group, the SOD activities of the liver and brain tissues of the aging mice in the positive control group, the medium-dose group, the high-dose group and the comparative example 1 group were significantly increased (P < 0.05), and the SOD activities of the liver and brain tissues of the aging mice in the low-dose group and the comparative example 2 group were increased, but there was no significant difference (P> 0.05). The SOD activity of the liver tissue of the high-dose group was significantly higher than that of the positive control group (P < 0.05); Figure 4 C, D, compared with the model control group, the MDA content in the liver and brain tissues of the aging mice in the positive control group, low-dose group, medium-dose group, and high-dose group were significantly reduced (P < 0.05), and the MDA content in the liver of the aging mice in the comparative example 1 and the MDA content in the brain of the aging mice in the comparative example 2 were significantly reduced (P < 0.05); Figure 4 E, F, Compared with the model control group, the CAT activity of the liver and brain tissues of aging mice intervened in the positive control group, low-dose group, medium-dose group, and high-dose group, as well as the CAT activity of the brain tissues of aging mice intervened in comparison example 2 were significantly increased (P < 0.05), the CAT activity of the liver and brain tissues of aging mice intervened in comparison example 1, and the liver tissues of aging mice intervened in comparison example 2 were increased, but there was no significant difference (P > 0.05), but the effects of the low-dose group, medium-dose group, and high-dose group, especially the high-dose group, were significantly better than those of comparison examples 1-2 groups.

[0107] In summary, the composition of the effective parts of traditional Chinese medicine of the present invention can significantly improve the activity of SOD and CAT in aging mice, reduce the MDA content, and thus scavenge superoxide anions, inhibit the damage of H2O2 to the liver and kidneys of D-galactose-aged mice, effectively improve the body's immunity, reduce the degree of oxidative damage in the body, effectively slow down oxidative damage, reduce the level of lipid peroxidation, and play a role in delaying aging, and the effect is better than that of the positive control group.

[0108] Example 6 - Effect of the effective fraction composition of traditional Chinese medicine of the present invention on serum inflammatory factor indicators in mice

[0109] Experimental Methods: Blood was collected from the mouse eyeballs and allowed to stand at room temperature for 30 minutes. The blood samples were centrifuged at 4°C and 2500 rpm for 10 minutes, and the supernatant was collected for later use. Mice were sacrificed by dislocation, and brain tissue was removed and weighed. Nine volumes of 4°C PBS buffer were added and homogenized in an ice-water bath. The homogenate was centrifuged at 4°C and 2500 rpm for 10 minutes, and the supernatant was collected for later use. Mouse serum levels of TNF-a, IL-6, and IL-1β were assayed according to the instructions in the respective kits.

[0110] The levels of inflammatory factors TNF-a, IL-6, and IL-1β in the serum of mice in each group were as follows: Figure 5 As shown, Figure 5AC are the results of the serum IL-6, TNF-α and IL-1β levels of mice in each group; Figure 5 The results show that:

[0111] Compared with the blank control group, the levels of inflammatory factors such as TNF-α, IL-6 and IL-1β in the serum of mice in the model control group increased significantly (P<0.05), indicating that the aging model was successfully established. Figure 5 A, compared with the model control group, the IL-6 content in the serum of the mice in the positive control group was significantly reduced (P<0.01), and the IL-6 content in the mice in the low-dose group, medium-dose group, high-dose group and comparative example 1-2 groups administered with the composition of the present invention was significantly reduced (P<0.05), and the IL-6 content in the high-dose group was lower than that in the positive control group but there was no significant difference (P>0.05); Figure 5 B. Compared with the model control group, the TNF-α content in the serum of the mice in the positive control group was significantly reduced (P<0.01). The TNF-α content in the mice in the low-dose group, medium-dose group, high-dose group and comparative example 1-2 groups administered with the composition of the present invention was significantly reduced (P<0.05). The IL-6 content in the high-dose group was significantly lower than that in the positive control group (P<0.05). Figure 5 C. Compared with the model control group, the IL-1β content in the serum of mice in the positive control group was significantly decreased (P<0.01), and the IL-1β content in the mice of the low-dose group, medium-dose group, high-dose group and comparative example 1-2 groups gavaged with the composition of the present invention was significantly decreased (P<0.05), while the IL-1β content in the high-dose group was significantly lower than that in the positive control group but there was no significant difference (P>0.05); the inflammatory factor content in the mice of the comparative example 1-2 group was significantly decreased (P<0.05), but the effect was not as good as that of the medium-dose group and high-dose group of the effective part composition of the traditional Chinese medicine of the present invention, indicating that the effective part composition of the traditional Chinese medicine of the present invention can reduce the level of inflammatory factors in D-galactose-aged mice, improve the level of inflammation in D-galactose-aged mice, and thus play a role in delaying aging, and the anti-inflammatory effect of the high-dose group is better than that of the positive control group.

[0112] Example 7 - Effect of the composition of effective parts of Chinese medicine of the present invention on the survival of mice

[0113] Experimental method: 40 mice were selected and randomly divided into a model control group, a positive control group, a low-dose group of the tablet prepared in Example 1, and a high-dose group (hereinafter referred to as the low-dose group and the high-dose group), with 10 mice in each group. The mice in each group were intraperitoneally injected with D-galactose (150 mg / kg). At the same time as the model was established, the low-dose group and the high-dose group were gavaged with different doses of the tablet prepared in Example 1 (respectively: 0.59 g / kg and 2.34 g / kg, with physiological saline solution as the solvent). The positive control group was gavaged with 0.1 g / kg of vitamin C, and the model group was gavaged with 0.2 mL of physiological saline solution, once a day. During the experiment, the death of mice in each group was observed and recorded.

[0114] The experimental results are as follows Figure 6 As shown by Figure 6 It can be seen that compared with the model control group, the survival time of mice in the positive control group, low-dose group and high-dose group was prolonged, indicating that the effective part composition of traditional Chinese medicine of the present invention can significantly prolong the survival time of mice and delay D-galactose-induced aging.

[0115] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Those skilled in the art will readily appreciate that various modifications and variations of the present invention are possible. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A composition of effective parts of traditional Chinese medicine for alleviating inflammatory aging, characterized in that: Calculated by weight, the invention comprises: 15-40 parts of cod polypeptide extract, 15-30 parts of oyster polysaccharide extract, 15-25 parts of total terpene extract of Eucommia ulmoides male flowers, 15-35 parts of deer blood polypeptide extract, 25-50 parts of ginsenoside extract, 10-20 parts of grape seed oil VE extract, 20-40 parts of Cordyceps militaris polysaccharide extract, 5-15 parts of Haematococcus pluvialis carotenoid extract, and 10-25 parts of krill oil astaxanthin extract.

2. The effective component composition of traditional Chinese medicine for alleviating inflammatory aging according to claim 1, characterized in that: The preparation method of the cod polypeptide extract is as follows: thawing the cod maw, washing it, cutting it into small pieces, soaking it in alkali solution, stirring it for a period of time, washing it with water until it is neutral, adding isopropyl alcohol to remove fat, and washing it to obtain the pretreated cod maw; The pretreated cod maw is extracted by adding it into water and then cooled to room temperature for homogenization. The pH is adjusted to 7.0 and then a composite protease is added for enzymatic hydrolysis. After the enzymatic hydrolysis is completed, the enzyme is inactivated by heating. After decolorization, the supernatant is centrifuged and subjected to a nanofiltration membrane circulation desalination treatment under pressure. The treated liquid is collected and concentrated to obtain the cod polypeptide extract.

3. The effective component composition of traditional Chinese medicine for alleviating inflammatory aging according to claim 1, characterized in that: The preparation method of the oyster polysaccharide extract comprises the following steps: washing and draining fresh oysters, boiling, cooling, grinding and homogenizing, adjusting the pH to 8.5-9.5, adding alkaline protease for enzymatic hydrolysis, heating to inactivate the enzyme after the enzymatic hydrolysis, taking the supernatant after centrifugation, adding the supernatant to anhydrous ethanol, centrifuging, collecting the precipitate, and freeze-drying to obtain crude oyster glycogen; and then using anion exchange column chromatography to separate, purify and elute the crude oyster glycogen to obtain the oyster polysaccharide extract.

4. The effective component composition of traditional Chinese medicine for alleviating inflammatory aging according to claim 1, characterized in that: The preparation methods of the total terpenes of Eucommia ulmoides male flowers, total saponins of Panax ginseng and polysaccharide extracts of Cordyceps militaris are the same, all of which adopt the following method: drying, crushing and sieving the male flowers of Eucommia ulmoides, Panax ginseng and Cordyceps militaris respectively; then extracting multiple times, combining the extracts, filtering and concentrating under reduced pressure to obtain concentrated solutions respectively; The concentrated solutions of Eucommia ulmoides male flowers and Panax ginseng are eluted with macroporous adsorption resin and then collected and concentrated to obtain the total terpene extract of Eucommia ulmoides male flowers and the total saponin extract of Panax ginseng respectively; The concentrated solution obtained from Cordyceps militaris is added with anhydrous ethanol and allowed to stand, and the precipitate is collected by centrifugation. The precipitate is filtered through a microfiltration membrane and a nanofiltration membrane, and then collected and concentrated to obtain the Cordyceps militaris polysaccharide extract.

5. The effective component composition of traditional Chinese medicine for alleviating inflammatory aging according to claim 1, characterized in that: The preparation method of the deer blood polypeptide extract comprises the following steps: freeze-drying deer blood plasma to prepare lyophilized powder, adding the lyophilized powder into water, performing ultrasonic treatment, adjusting the pH to 7-8, adding a mixed enzyme solution for enzymatic hydrolysis, heating to inactivate the enzyme after the enzymatic hydrolysis, cooling to room temperature, centrifuging, collecting the supernatant, and concentrating to obtain the deer blood polypeptide extract.

6. The effective component composition of traditional Chinese medicine for alleviating inflammatory aging according to claim 1, characterized in that: The preparation method of the grape seed oil VE extract comprises the following steps: deodorizing grape seed crude oil to obtain a distillate, adding acidic methanol to the distillate for mixing and saponification, adding alkali solution for neutralization, and then standing to separate the distillate, taking the oil phase and cold-precipitating and crystallizing it under low temperature conditions, distilling the obtained filtrate to obtain a concentrate, and molecularly distilling and concentrating the concentrate to obtain the grape seed oil VE extract.

7. The effective component composition of traditional Chinese medicine for alleviating inflammatory aging according to claim 1, characterized in that: The preparation method of the Haematococcus pluvialis carotenoid extract comprises the following steps: drying the Haematococcus pluvialis and then crushing it, passing it through a 20-40 mesh sieve, adding the obtained algae powder to an enzyme solution, adjusting the pH to 4.0, breaking the algae wall, centrifuging, discarding the supernatant, adding acetone for extraction, centrifuging again, discarding the lower precipitate, and purifying the solution to obtain the Haematococcus pluvialis carotenoid extract.

8. The effective component composition of traditional Chinese medicine for alleviating inflammatory aging according to claim 1, characterized in that: The preparation method of the krill oil astaxanthin extract comprises the following steps: thawing the krill, drying the krill until the water content is less than 10%, crushing, screening, extracting, and filtering, removing the solvent from the resulting filtrate to obtain krill oil, dissolving the krill oil, applying the dissolved oil to a silica gel chromatography column, eluting, collecting the eluate, and concentrating the eluate to obtain the krill oil astaxanthin extract.

9. A Chinese medicine preparation for alleviating inflammatory aging, characterized in that: Comprising the effective part composition of traditional Chinese medicine according to any one of claims 1 to 8 and pharmaceutically acceptable excipients; The effective parts of the traditional Chinese medicine composition account for 40-80% of the total weight of the preparation.

10. Use of the effective fraction composition of traditional Chinese medicine according to any one of claims 1 to 8 in the preparation of anti-aging medicines, health products or cosmetics.

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