Method and device for preparing amino acid water-soluble fertilizer from dead animals

Abstract

The invention relates to the technical field of water-soluble fertilizer processing, in particular to a method and a device for preparing an amino acid water-soluble fertilizer from animals died of illness. The method comprises the following steps: raw material treatment, medium and low temperature cooking, biological fermentation, enzymolysis treatment, chelation reaction and product generation. Through biological fermentation and enzymolysis technologies, the use of chemical acid is avoided, the generation of wastewater and waste gas is remarkably reduced, and the treatment process is more environment-friendly. Meanwhile, the optimized technological process simplifies the production process, reduces the consumption of equipment and energy, improves the release rate of amino acid and the absorptivity of the fertilizer, and ensures the high quality of the product. Experimental data show that compared with crops using traditional fertilizers, the yield of the crops using the amino acid water-soluble fertilizer prepared by the invention is increased by 15-20%, and the quality is also remarkably improved.

Description

Technical Field

[0001] The invention relates to the technical field of water-soluble fertilizer processing, in particular to a method and a preparation device for preparing amino acid water-soluble fertilizer by utilizing dead animals. Background Art

[0002] Amino acids are the smallest organic structure of proteins in all organisms. Multiple amino acid molecules are connected to form polypeptides, and multiple polypeptide chains are folded in space to form proteins. Therefore, the production of amino acids from dead animals has become a new research hotspot. At present, the main methods used to produce amino acids from dead animals are acid hydrolysis, alkaline hydrolysis and enzymatic hydrolysis. However, the amino acids obtained by acid hydrolysis have low content and strong odor; the amino acids produced by alkaline hydrolysis undergo optical isomerization, and the products are D-type amino acids, which are difficult to decompose and difficult to be absorbed and utilized, and have low nutritional value; enzymatic hydrolysis method has incomplete hydrolysis, many intermediate products, and a long operation cycle.

[0003] Patent CN115626847A proposes a process for rapidly acid-hydrolyzing dead animals to produce water-soluble amino acid fertilizer. The core of this process is the use of chemical acids (such as hydrochloric acid and sulfuric acid) to rapidly hydrolyze dead animals, breaking down proteins into amino acids. This process is characterized by its rapid acid hydrolysis, enabling the protein to be broken down in a relatively short period of time.

[0004] However, this method is used to process water-soluble fertilizers and has the following problems:

[0005] 1. The acid hydrolysis process requires a large amount of chemical acid and high temperature conditions, which produces a large amount of wastewater and waste gas, which contain heavy metals and organic pollutants. It is difficult to treat and easily causes secondary pollution;

[0006] 2. Although this method can quickly acidify dead animals, it fails to fully tap the resource value of dead animals. The extraction rate and purity of amino acids are limited, and they cannot be converted into high-value-added water-soluble fertilizers. Summary of the Invention

[0007] (1) Technical problems solved

[0008] In view of the deficiencies in the prior art, the present invention provides a method and a device for preparing amino acid water-soluble fertilizer using dead animals, which solves the problems raised by the above-mentioned background technology.

[0009] (2) Technical solution

[0010] To achieve the above objectives, the present invention is implemented through the following technical solutions: A method for preparing amino acid water-soluble fertilizer using dead animals, comprising the following steps:

[0011] S1. Collect dead animal carcasses, perform preliminary cleaning and remove impurities, freeze and crush the cleaned raw materials at low temperature, and steam the crushed raw materials at medium to low temperature. The impurity removal work mainly involves removing ear tags, chains, ropes on the dead animal carcasses, as well as stones, glass, ceramics or other foreign objects brought in from the pens by forklifts.

[0012] S2, mixing the cooked raw materials with the composite bacteria and performing anaerobic fermentation, and regularly introducing micro-oxygen during the fermentation process;

[0013] S3, adding optimized complex enzymes to the fermented product for enzymatic hydrolysis, and using ultrasonic assisted treatment during the enzymatic hydrolysis process;

[0014] S4, mixing the enzymatic hydrolysis product with a chelating agent to perform a chelation reaction;

[0015] S5. Filter and concentrate the obtained product to obtain high-quality amino acid water-soluble fertilizer, and add nutritional elements and bioactive substances to the product.

[0016] Preferably, in step S1, rosemary extract or vitamin E is added during the crushing process, and citric acid or antioxidant is added during the cooking process.

[0017] Preferably, in step S2, the composite bacteria is a mixture of lactic acid bacteria, yeast and Bacillus in a mass ratio of 1: (1.2-2.2): (0.8-1.2).

[0018] Preferably, in step S2, the fermentation temperature is set to 35-40° C., and the fermentation time is set to 7-10 days.

[0019] Preferably, in step S3, the complex enzyme is a mixture of alkaline protease, cellulase and β-glucanase in a mass ratio of 2: (0.8-1.2): (1.2-1.4), the enzymolysis temperature is controlled at 45-50° C., and the enzymolysis time is set to 4-6 hours.

[0020] Preferably, in step S4, the chelating agent is a mixture of sugar alcohol, citric acid and EDTA in a mass ratio of 2: (0.5-1.2): (0.8-1.1), the reaction temperature is set at 50-60° C., the reaction time is set at 2-3 hours, and stirring is performed regularly during the reaction.

[0021] Preferably, the nutrients include one or more of calcium, magnesium, and zinc, and the bioactive substance is humic acid or seaweed extract.

[0022] A device for preparing amino acid water-soluble fertilizer using dead animals, applied to the above-mentioned method for preparing amino acid water-soluble fertilizer using dead animals, comprises:

[0023] Raw material processing unit, used for preliminary processing of dead animals, including cleaning, low-temperature crushing and antiseptic treatment to preserve the nutrients of the raw materials and prevent spoilage;

[0024] Medium-low temperature cooking unit, used to cook the crushed raw materials under medium-low temperature conditions to kill pathogens and extract some nutrients while reducing nutritional losses;

[0025] The biological fermentation unit is used for anaerobic fermentation under controlled temperature and oxygen conditions, promoting the growth of beneficial bacteria by adding composite bacteria to decompose organic matter in the raw materials;

[0026] The enzymatic hydrolysis unit is used to perform enzymatic hydrolysis at a specific temperature. By adding complex enzymes and ultrasonic waves to improve the efficiency of enzymatic hydrolysis, it can further decompose proteins and other macromolecules in the raw materials.

[0027] The chelation reaction unit is used to mix the enzymatic hydrolysis product with the chelating agent to form a stable chelate structure, thereby enhancing the absorption rate and stability of the fertilizer;

[0028] The product collection unit is used to filter, concentrate and package the reaction products to obtain high-quality amino acid water-soluble fertilizer;

[0029] Control system, used to automatically control parameters such as temperature, time, stirring speed, etc. during the entire preparation process to ensure the accuracy and consistency of the operation;

[0030] Safety protection system, used to monitor and ensure the safety of the entire preparation process, including disinfection and emergency response mechanisms.

[0031] (3) Beneficial effects

[0032] The present invention provides a method and device for preparing amino acid water-soluble fertilizer using dead animals, which has the following beneficial effects:

[0033] 1. The present invention avoids the use of chemical acid through biological fermentation and enzymatic hydrolysis technology, significantly reduces the generation of wastewater and waste gas, and the treatment process is more environmentally friendly. The low-temperature crushing and medium-low temperature cooking steps retain the nutritional components of the raw materials and kill pathogens at the same time, ensuring the safety and environmental friendliness of the product. By converting dead animals into high-value amino acid water-soluble fertilizers, the recycling of resources is achieved, the pressure of dead animals on the environment is reduced, high-quality fertilizers are provided for agricultural production, and a win-win situation of economic and environmental benefits is achieved.

[0034] 2. The present invention combines bio-fermentation and enzymatic hydrolysis through an optimized process flow, simplifying the entire production process and reducing equipment and energy consumption. The modular design enables each unit to work together, reducing equipment redundancy and further reducing production costs. Ultrasonic-assisted enzymatic hydrolysis technology improves enzymatic hydrolysis efficiency and reduces processing time, making the entire process more efficient and economical, suitable for large-scale industrial production.

[0035] 3. The amino acid water-soluble fertilizer prepared by the present invention has a high amino acid content and is rich in various nutrients and bioactive substances. It has a significant fertilizer effect. Through ultrasound-assisted enzymatic hydrolysis and chelation technology, the release rate of amino acids and the absorption rate of fertilizer are improved, ensuring the high quality of the product. Experimental data show that the yield of crops using the amino acid water-soluble fertilizer prepared by the present invention is 15%-20% higher than that of crops using traditional fertilizers, and the quality is also significantly improved. The application of chelation technology further enhances the stability and absorption rate of the fertilizer, reduces nutrient loss, and ensures the efficient use of the fertilizer. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 It is a schematic diagram of the process of the present invention. DETAILED DESCRIPTION

[0037] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0038] Example 1:

[0039] like Figure 1 As shown, an embodiment of the present invention provides a method for preparing amino acid water-soluble fertilizer using dead animals, comprising:

[0040] Raw material processing: Dead pig carcasses are collected and initially cleaned and decontaminated to remove surface dirt. The cleaned raw materials are then cryogenically frozen and pulverized (-25°C) to preserve their nutritional content and structural integrity. Rosemary extract is added during the pulverization process to provide a preservative and antioxidant effect.

[0041] Steaming at medium to low temperature: Steam the crushed raw materials at medium to low temperature (65°C) to kill pathogens and extract some nutrients. Add a small amount of citric acid during the cooking process to reduce the loss of nutrients.

[0042] For biological fermentation, the cooked ingredients are mixed with a bacterial complex (lactic acid bacteria, yeast, and Bacillus, in a mass ratio of 1:2:1) for anaerobic fermentation. Micro-oxygenation is periodically introduced during the fermentation process to promote the growth and metabolism of beneficial bacteria. The fermentation temperature is controlled at 38°C and the fermentation time is 8 days.

[0043] The product after enzymatic hydrolysis and fermentation was then treated with a complex enzyme (alkaline protease, cellulase, and β-glucanase in a mass ratio of 2:1:1) for enzymatic hydrolysis. Ultrasonic treatment was used to enhance the efficiency of the hydrolysis. The hydrolysis temperature was maintained at 48°C for 5 hours.

[0044] Chelation reaction: Mix the enzymatic hydrolysis product with a chelating agent (sugar alcohol, citric acid, and EDTA, in a mass ratio of 2:1:1) for a chelation reaction. Maintain the reaction temperature at 55°C for 2.5 hours. Stir regularly during the reaction to ensure uniformity of the chelation reaction.

[0045] After the above steps, the product is filtered and concentrated to obtain high-quality amino acid water-soluble fertilizer. Nutrients such as calcium, magnesium, and zinc, as well as bioactive substances such as humic acid and seaweed extract are added to the product to enhance the fertilizer's multifunctionality.

[0046] A device for preparing amino acid water-soluble fertilizer using dead animals, applied to the above-mentioned method for preparing amino acid water-soluble fertilizer using dead animals, comprises:

[0047] Raw material processing unit, used for preliminary processing of dead animals, including cleaning, low-temperature crushing and antiseptic treatment to preserve the nutrients of the raw materials and prevent spoilage;

[0048] Medium-low temperature cooking unit, used to cook the crushed raw materials under medium-low temperature conditions to kill pathogens and extract some nutrients while reducing nutritional losses;

[0049] The biological fermentation unit is used for anaerobic fermentation under controlled temperature and oxygen conditions, promoting the growth of beneficial bacteria by adding composite bacteria to decompose organic matter in the raw materials;

[0050] The enzymatic hydrolysis unit is used to perform enzymatic hydrolysis at a specific temperature. By adding complex enzymes and ultrasonic waves to improve the efficiency of enzymatic hydrolysis, it can further decompose proteins and other macromolecules in the raw materials.

[0051] The chelation reaction unit is used to mix the enzymatic hydrolysis product with the chelating agent to form a stable chelate structure, thereby enhancing the absorption rate and stability of the fertilizer;

[0052] The product collection unit is used to filter, concentrate and package the reaction products to obtain high-quality amino acid water-soluble fertilizer;

[0053] Control system, used to automatically control parameters such as temperature, time, stirring speed, etc. during the entire preparation process to ensure the accuracy and consistency of the operation;

[0054] Safety protection system, used to monitor and ensure the safety of the entire preparation process, including disinfection and emergency response mechanisms.

[0055] Example 2:

[0056] The difference between this embodiment and the first embodiment is that:

[0057] Raw material processing: Collect the carcasses of dead chickens and perform low-temperature freezing and crushing (-30℃).

[0058] Steaming at medium-low temperature: the cooking temperature is 70℃ and the time is 30 minutes.

[0059] Biological fermentation: The fermentation temperature is controlled at 35°C and the fermentation time is 7 days.

[0060] Enzymatic hydrolysis treatment: The enzymatic hydrolysis temperature was controlled at 50°C for 4 hours.

[0061] Chelating reaction: The reaction temperature was controlled at 60°C and the reaction time was 3 hours.

[0062] Product generation: The amino acid content of the obtained amino acid water-soluble fertilizer reaches 22.3%.

[0063] Example 3:

[0064] The difference between this embodiment and the first embodiment is that:

[0065] Raw material processing: Collect the dead cattle carcasses and freeze and crush them at low temperature (-20℃). Medium-low temperature cooking: The cooking temperature is 65℃ and the time is 40 minutes.

[0066] Biological fermentation: The fermentation temperature is controlled at 37°C and the fermentation time is 9 days.

[0067] Enzymatic hydrolysis treatment: The enzymatic hydrolysis temperature was controlled at 47°C for 5 hours.

[0068] Chelating reaction: The reaction temperature was controlled at 53°C and the reaction time was 2 hours.

[0069] Product generation: The amino acid content of the obtained amino acid water-soluble fertilizer reaches 21.0%.

[0070] Example 4:

[0071] This embodiment differs from the first embodiment in that:

[0072] Raw material processing: Collect the dead sheep carcasses and freeze and crush them at low temperature (-28℃). Medium-low temperature cooking: The cooking temperature is 68℃ and the time is 35 minutes.

[0073] Biological fermentation: The fermentation temperature is controlled at 39°C and the fermentation time is 8 days.

[0074] Enzymatic hydrolysis: The enzymatic hydrolysis temperature was controlled at 49°C for 5 hours.

[0075] Chelating reaction: The reaction temperature was controlled at 58°C and the reaction time was 2.5 hours.

[0076] Product generation: The amino acid content of the obtained amino acid water-soluble fertilizer reaches 21.5%.

[0077] Embodiment 5:

[0078] This embodiment differs from the first embodiment in that:

[0079] Raw material processing: Collect dead dog carcasses and freeze and crush them at low temperature (-22℃). Medium-low temperature cooking: The cooking temperature is 62℃ and the time is 45 minutes.

[0080] Biological fermentation: The fermentation temperature is controlled at 36°C and the fermentation time is 10 days. Enzymatic hydrolysis: The enzymatic hydrolysis temperature is controlled at 46°C and the time is 6 hours.

[0081] Chelating reaction: The reaction temperature was controlled at 52°C and the reaction time was 3 hours.

[0082] Product generation: The amino acid content of the obtained amino acid water-soluble fertilizer reaches 20.8%.

[0083] Table 1 shows the amino acid yields and resource consumption of different embodiments, and compares them with conventional technologies to verify the superiority of the method of the present invention.

[0084] Table 1

[0085]

[0086] Conclusion From the table data, it can be seen that the preparation method of amino acid water-soluble fertilizer of the present invention is superior to conventional technology in terms of amino acid yield, energy consumption and environmental protection.

[0087] 1. The amino acid yield is significantly improved: the amino acid yield of the method of the present invention is between 20.8% and 22.5%, which is 30% to 40% higher than the 15.0% of the conventional technology.

[0088] 2. Significantly reduced energy consumption: The energy consumption of the method of the present invention is 0.75-0.85 kWh / kg, which is 29%-38% lower than the 1.2 kWh / kg of conventional technology.

[0089] 3. The amount of wastewater and waste gas generated is significantly reduced: the amount of wastewater generated by the method of the present invention is 0.25-0.35L / kg, and the amount of waste gas generated is 0.15-0.25m 3 / kg, which were significantly lower than the 1.0L / kg and 0.8m 3 / kg.

[0090] In summary, the method of the present invention has significant advantages in resource utilization efficiency, environmental protection and economic benefits, and is suitable for large-scale industrial production.

[0091] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A method for preparing amino acid water-soluble fertilizer using dead animals, characterized in that: The following steps are involved: S1. Collect the carcasses of dead animals, perform preliminary cleaning and impurity removal, freeze and crush the cleaned raw materials at low temperature, and steam and cook the crushed raw materials at medium and low temperature. S2, mixing the cooked raw materials with the composite bacteria and performing anaerobic fermentation, and regularly introducing micro-oxygen during the fermentation process; S3, adding optimized complex enzymes to the fermented product for enzymatic hydrolysis, and using ultrasonic assisted treatment during the enzymatic hydrolysis process; S4, mixing the enzymatic hydrolysis product with a chelating agent to perform a chelation reaction; S5. Filter and concentrate the obtained product to obtain high-quality amino acid water-soluble fertilizer, and add nutritional elements and bioactive substances to the product.

2. The method for preparing amino acid water-soluble fertilizer using dead animals according to claim 1, wherein: In step S1, rosemary extract or vitamin E is added during the crushing process, and citric acid or antioxidant is added during the cooking process.

3. The method for preparing amino acid water-soluble fertilizer using dead animals according to claim 1, wherein: In step S2, the composite bacteria is a mixture of lactic acid bacteria, yeast and bacillus in a mass ratio of 1: (1.2-2.2): (0.8-1.2).

4. The method for preparing amino acid water-soluble fertilizer using dead animals according to claim 1, wherein: In step S2, the fermentation temperature is set to 35-40° C., and the fermentation time is set to 7-10 days.

5. The method for preparing amino acid water-soluble fertilizer using dead animals according to claim 1, wherein: In step S3, the complex enzyme is a mixture of alkaline protease, cellulase and β-glucanase in a mass ratio of 2: (0.8-1.2): (1.2-1.4), the enzymolysis temperature is controlled at 45-50° C., and the enzymolysis time is set to 4-6 hours.

6. The method for preparing amino acid water-soluble fertilizer using dead animals according to claim 1, wherein: In step S4, the chelating agent is a mixture of sugar alcohol, citric acid and EDTA in a mass ratio of 2: (0.5-1.2): (0.8-1.1), the reaction temperature is set at 50-60° C., the reaction time is set at 2-3 hours, and stirring is performed regularly during the reaction.

7. The method for preparing amino acid water-soluble fertilizer using dead animals according to claim 1, wherein: The nutrients include one or more of calcium, magnesium, and zinc, and the bioactive substance is humic acid or seaweed extract.

8. A device for preparing amino acid water-soluble fertilizer using dead animals, applied to the method for preparing amino acid water-soluble fertilizer using dead animals according to claims 1-7, characterized in that: include: Raw material processing unit, used for preliminary processing of dead animals, including cleaning, low-temperature crushing and antiseptic treatment to preserve the nutrients of the raw materials and prevent spoilage; Medium-low temperature cooking unit, used to cook the crushed raw materials under medium-low temperature conditions to kill pathogens and extract some nutrients while reducing nutritional losses; The biological fermentation unit is used for anaerobic fermentation under controlled temperature and oxygen conditions, promoting the growth of beneficial bacteria by adding composite bacteria to decompose organic matter in the raw materials; The enzymatic hydrolysis unit is used to perform enzymatic hydrolysis at a specific temperature. By adding complex enzymes and ultrasonic waves to improve the efficiency of enzymatic hydrolysis, it can further decompose proteins and other macromolecules in the raw materials. The chelation reaction unit is used to mix the enzymatic hydrolysis product with the chelating agent to form a stable chelate structure, thereby enhancing the absorption rate and stability of the fertilizer; The product collection unit is used to filter, concentrate and package the reaction products to obtain high-quality amino acid water-soluble fertilizer; Control system, used to automatically control parameters such as temperature, time, stirring speed, etc. during the entire preparation process to ensure the accuracy and consistency of the operation; Safety protection system, used to monitor and ensure the safety of the entire preparation process, including disinfection and emergency response mechanisms.

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