Enzyme preparation extraction device

Abstract

The invention discloses an enzymic preparation extraction device, and relates to the technical field of enzymic preparation extraction devices, the enzymic preparation extraction device comprises a crushing and separating mechanism mounted on a bracket body; the crushing and separating mechanism comprises a crushing tank body, and a crusher is mounted above the crushing tank body in a matched manner; the crushing tank body is fixed above the filtering tank body; an on-off assembly is arranged in the filtering tank body; according to the invention, a fermented biological material is put into the crushing tank body and is crushed by the crusher; a biological material is crushed by the crusher, and cells are crushed by shear force and impact force, so that subsequent enzyme extraction is facilitated. When a biological material is subjected to filter pressing, the biological material in the filter membrane is extruded from all directions due to the mechanical pressure of the sliding disc and the pressure generated by compressed air in the filter tank body, so that the enzyme liquid filtering effect of the biological material is improved.

Description

Technical Field

[0001] The present invention specifically relates to the technical field of enzyme preparation extraction devices, in particular to an enzyme preparation extraction device. Background Art

[0002] Enzyme extraction is the process of isolating and purifying target enzymes from biological materials (such as microorganisms, animal and plant tissues). Its core goal is to remove impurities and concentrate the enzyme components while maintaining enzyme activity. Depending on the enzyme's source (e.g., microbial fermentation broth, animal liver, plant seeds, etc.), raw materials with high enzyme content and low impurities are selected. For example, α-amylase is often produced from Bacillus subtilis fermentation broth, while cellulase is often produced from fungal fermentation broth.

[0003] Chinese patent application No. 202321011274.1 discloses a fine filtration device for extracting enzyme preparations, including a cylinder, the bottom of the inner cavity of the cylinder is rotatably connected to a rotating shaft through a bearing, the bottom of the rotating shaft passes through the cylinder and is fixedly connected to a slave pulley, a rotating motor is fixedly installed on the left side of the bottom of the inner cavity of the cylinder, and the output end of the rotating motor is fixedly connected to a main pulley.

[0004] The fine filtration device for enzyme preparation extraction in the above patent filters the enzyme solution by centrifugation. However, this device that relies solely on centrifugation for filtration cannot achieve a good filtration effect. A large amount of enzyme still remains in the biological material, and the separation is not complete, thereby causing waste of biological materials. Summary of the Invention

[0005] The purpose of the present invention is to provide an enzyme preparation extraction device with the functions of biomaterial crushing, high-pressure filtration, low-temperature centrifugation and concentration, which greatly meets the process requirements of enzyme preparation and has extremely high enzyme extraction efficiency; so as to solve the technical problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] An enzyme preparation extraction device includes a crushing and separating mechanism mounted on a support body; the crushing and separating mechanism includes a crushing tank body, a crusher is mounted above the crushing tank body; the crushing tank body is fixed above a filtering tank body; an on-off assembly is provided inside the filtering tank body;

[0008] The on-off assembly comprises a sliding plate that slides up and down along the inner wall of the filter tank, a cross slot is provided on the upper surface of the sliding plate, and a slider is slidably connected in the cross slot; the slider is movably connected to the movable arm via a rotating shaft; the upper end of the movable arm is movably connected to the metal ring via a rotating shaft; an elastic membrane is fixed in the middle of the metal ring; and a protective shell is welded to the outer ring of the bottom of the metal ring;

[0009] A focusing cover is fixed at the bottom of the sliding plate; a filter membrane is fixed to the bottom of the focusing cover through a flange, and a rubber bellows is sleeved on the outside of the filter membrane; the bottoms of the rubber bellows and the filter membrane are both sealed and fixed to the support member; a liquid outlet pipe connected to the liquid inlet end of the delivery pump is fixed to one side of the lower end of the rubber bellows through a flange.

[0010] As a further technical solution of the present invention, a first air inlet pipe is further provided on the top of the crushing tank body; and a second air inlet pipe is provided on the bottom of the filtering tank body.

[0011] As a further technical solution of the present invention, a cross bracket is welded inside the condenser, and a cross top rod is slidably connected in the middle of the cross bracket, and a spring is sleeved on the outside of the cross top rod; the top of the cross top rod is threadedly connected to the screw sleeve bonded and arranged in the middle of the elastic membrane; the lower end of the cross top rod passes through the bottom of the support member and is slidably connected.

[0012] As a further technical solution of the present invention, a metal block is fixed to the lower end of the cross push rod; and an electromagnet for attracting the metal block is installed in the middle of the bottom of the support member.

[0013] As a further technical solution of the present invention, the liquid outlet of the delivery pump is connected to an enzyme liquid delivery pipe, and the upper end of the enzyme liquid delivery pipe is connected to a low-temperature centrifuge; the low-temperature centrifuge is connected to a concentration tank through the delivery pipe; and a booster pump is also connected in series to the delivery pipe.

[0014] As a further technical solution of the present invention, the low-temperature centrifuge has a casing with a sandwich layer, and the sandwich layer of the casing is connected to a cooling pipe; the cooling pipe is connected to an air cooling or water cooling device.

[0015] A rotating motor is fixed on the top of the casing, and the shaft of the rotating motor passes through the inside of the casing and is connected with the centrifugal screen; the enzyme liquid delivery pipe is located above the centrifugal screen.

[0016] As a further technical solution of the present invention, the concentration tank is fixedly mounted on the frame; a controller is also fixed on one side of the frame.

[0017] As a further technical solution of the present invention, the concentration tank is a sandwich tank with a heating coil provided in the interlayer; a stirring device is provided inside the concentration tank, and a pressure gauge, a pressure relief valve, a safety valve and a sampling port are installed on the tank top cover.

[0018] As a further technical solution of the present invention, when the electromagnet and the metal block are attracted, the cross push rod compresses the spring and lifts the elastic membrane, and the slider moves along the cross slide groove toward the axis of the sliding disk. At this time, the metal ring is separated from the sliding disk, and a cavity is formed between the metal ring and the sliding disk to facilitate the enzyme solution to enter the filter membrane;

[0019] When the electromagnet and the metal block separate, the spring pushes the cross rod downward, the elastic membrane resets, the metal ring and the sliding disk close, cutting off the flow of the enzyme liquid. At this time, the enzyme liquid is stored in the crushing tank and crushed by the crusher.

[0020] As a further technical solution of the present invention, an annular groove is provided on the outer edge of the sliding plate, an O-type rubber ring is embedded in the annular groove and fixed by glue.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] 1. In the present invention, the fermented biological material is placed in a crushing tank and crushed by a crusher; the biological material is crushed by the crusher, and the cells are crushed by shear force and impact force, thereby facilitating the subsequent enzyme extraction.

[0023] 2. In the present invention, when the biological material is filtered, there is not only the mechanical pressure of the sliding disk, but also the pressure generated by the compressed air inside the filter tank, which squeezes the biological material in the filter membrane from all directions, thereby improving the effect of the biological material filtering the enzyme solution.

[0024] 3. In the present invention, the enzyme solution produced after filtration is transported to a low-temperature centrifuge through a delivery pump and an enzyme solution delivery tube. The low temperature can inhibit the reproduction of residual microorganisms in the raw material, prevent the proteases or nucleases secreted by them from degrading the target enzyme, and at the same time reduce the activity of endogenous enzymes (such as tissue autolytic enzymes) in the raw material to prevent the enzyme solution from deteriorating. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention.

[0026] Figure 2 In the present invention Figure 1 Schematic diagram of the bottom structure.

[0027] Figure 3 In the present invention Figure 1 main view.

[0028] Figure 4 In the present invention Figure 3 AA cross-sectional view.

[0029] Figure 5 It is a schematic diagram of the installation of the filter tank and the on-off assembly in the present invention.

[0030] Figure 6 It is a structural diagram of the switch component in the present invention.

[0031] Figure 7 It is a schematic diagram of the internal structure of the switch component in the present invention.

[0032] Figure 8 In the present invention Figure 7 main view.

[0033] Figure 9 In the present invention Figure 8 BB cross-sectional view.

[0034] Figure 10 It is a schematic diagram of the internal structure of the low-temperature centrifuge of the present invention.

[0035] In the figure: 1- bracket body, 2- crushing and separation mechanism, 3- low-temperature centrifuge, 4- frame body, 5- concentration tank, 6- delivery pump, 7- enzyme liquid delivery pipe, 8- cooling pipe, 9- delivery pipe, 10- booster pump, 11- controller;

[0036] 21-crushing tank, 22-crusher, 23-filtering tank, 24-on / off assembly, 25-filter membrane, 26-rubber bellows, 27-support, 28-first air inlet pipe, 29-second air inlet pipe;

[0037] 241-sliding plate, 242-cross slide, 243-slider, 244-movable arm, 245-metal ring, 246-elastic membrane, 247-shield, 248-flow collecting cover, 249-cross push rod, 2410-spring, 2411-metal block, 2412-electromagnet, 2413-cross bracket. DETAILED DESCRIPTION

[0038] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0039] See also Figures 1 to 10 In an embodiment of the present invention, an enzyme preparation extraction device includes a crushing and separating mechanism 2 mounted on a support body 1; the crushing and separating mechanism 2 includes a crushing tank body 21, and a crusher 22 is mounted above the crushing tank body 21; the crushing tank body 21 is fixed above a filtering tank body 23; and an on-off component 24 is provided inside the filtering tank body 23;

[0040] The on-off assembly 24 comprises a sliding plate 241 that slides up and down along the inner wall of the filter tank 23. A cross slot 242 is formed on the upper surface of the sliding plate 241, and a slider 243 is slidably connected in the cross slot 242. The slider 243 is movably connected to a movable arm 244 via a rotating shaft. The upper end of the movable arm 244 is movably connected to a metal ring 245 via a rotating shaft. An elastic membrane 246 is fixed in the middle of the metal ring 245. A protective shell 247 is also welded to the outer ring of the bottom of the metal ring 245.

[0041] A focusing cover 248 is fixed to the bottom of the sliding disk 241; a filter membrane 25 is fixed to the bottom of the focusing cover 248 through a flange, and a rubber bellows 26 is sleeved on the outside of the filter membrane 25; the bottoms of the rubber bellows 26 and the filter membrane 25 are both sealed and fixed to the support member 27; a liquid outlet pipe connected to the liquid inlet end of the delivery pump 6 is fixed to one side of the lower end of the rubber bellows 26 through a flange.

[0042] By adopting the above technical solution, under normal conditions, when the electromagnet 2412 and the metal block 2411 are separated, the spring 2410 pushes the cross push rod 249 downward, the elastic membrane 246 is in a horizontal state, the metal ring 245 and the sliding disk 241 are closed, and the protective shell 247 is in the gap between the metal ring 245 and the sliding disk 241, cutting off the flow of the biological material; then the fermented biological material is placed in the crushing tank 21 and crushed by the crusher 22.

[0043] The biomaterial is broken by the disruptor 22, and the cells are broken by shear force and impact force, thereby facilitating the subsequent extraction of enzymes.

[0044] Please see the attached Figure 3 and Figure 6-9 In this embodiment, a first air inlet pipe 28 is further provided at the top of the crushing tank body 21; a second air inlet pipe 29 is provided at the bottom of the filtering tank body 23; a cross bracket 2413 is welded inside the focusing cover 248, and a cross top rod 249 is slidably connected in the middle of the cross bracket 2413, and a spring 2410 is sleeved on the outside of the cross top rod 249; the top of the cross top rod 249 is threadedly connected to the screw sleeve bonded and arranged in the middle of the elastic membrane 246; the lower end of the cross top rod 249 passes through the bottom of the support member 27 and is slidably connected.

[0045] A metal block 2411 is fixed to the lower end of the cross rod 249 ; an electromagnet 2412 for attracting the metal block 2411 is also installed at the middle position of the bottom of the support member 27 .

[0046] By adopting the above technical solution, after the biomaterial is crushed, the electromagnet 2412 and the metal block 2411 are attracted, the cross push rod 249 compresses the spring 2410, and lifts the elastic membrane 246, and the slider 243 moves along the cross slot 242 toward the axis of the sliding disk 241. At this time, the metal ring 245 is separated from the sliding disk 241, and a cavity is formed between the metal ring 245 and the sliding disk 241 to facilitate the entry of the enzyme solution into the filter membrane 25;

[0047] The elastic membrane 246 is lifted up in a conical shape, and the crushed biological material flows around along the conical elastic membrane 246 and then enters the filter membrane 25 between the metal ring 245 and the sliding disk 241. Then, the electromagnet 2412 loses power, and the cross rod 249 moves downward under the weight of the metal block 2411, and the filter membrane 25 returns to its original shape, closing between the metal ring 245 and the sliding disk 241.

[0048] Subsequently, compressed gas is introduced into the first air inlet pipe 28 (the second air inlet pipe is closed). As the air pressure in the crushing tank 21 continues to increase, the sliding plate 241 slides downward under the push of the air pressure and compresses the rubber bellows 26 and the filter membrane 25. The biological material in the filter membrane 25 is filtered by the filter membrane 25 and then enters the gap between the rubber bellows 26 and the filter membrane 25, and is output through the delivery pump 6.

[0049] When the biological material is filtered, there is not only mechanical pressure from the sliding plate 241, but also pressure from the compressed air inside the filter tank 23, which squeezes the biological material in the filter membrane 25 from all directions, thereby improving the effect of the biological material filtering the enzyme solution.

[0050] When the next round of biomaterial crushing is carried out, the first air inlet pipe 28 stops supplying air and the second air inlet pipe 29 starts supplying air. The compressed gas pushes the sliding plate 241 to slide upward along the filter tank body 23. When the sliding plate 241 contacts the raised ring inside the filter tank body 23, it stops moving. At this time, the sliding plate 241 and the protective shell 247 are in a closed state, and biomaterial can be added to the crushing tank body 21 for the next crushing process.

[0051] Please see the attached Figure 1-3 In this embodiment, the liquid outlet end of the delivery pump 6 is connected to the enzyme liquid delivery pipe 7, and the upper end of the enzyme liquid delivery pipe 7 is connected to the low-temperature centrifuge 3; the low-temperature centrifuge 3 is connected to the concentration tank 5 through the delivery pipe 9; and the delivery pipe 9 is also connected in series with a booster pump 10.

[0052] The low-temperature centrifuge 3 has a casing with a sandwich layer, and the sandwich layer of the casing is connected to a cooling pipe 8; the cooling pipe 8 is connected to an air cooling or water cooling device.

[0053] A rotating motor is fixed on the top of the casing, and the shaft of the rotating motor passes through the interior of the casing and is connected to the centrifugal screen; the enzyme liquid delivery pipe 7 is located above the centrifugal screen.

[0054] By adopting the above technical solution, the enzyme solution produced after filtration is transported to the low-temperature centrifuge 3 through the delivery pump 6 and the enzyme solution delivery pipe 7, and a refrigerant is provided to the interlayer of the low-temperature centrifuge 3 through the cooling pipe 8 to ensure that the temperature of the low-temperature centrifuge 3 is maintained at 4-10°C.

[0055] Most enzymes are easily inactivated due to conformational changes at room temperature or high temperature (such as protein denaturation). Low temperature (usually controlled at 4-10°C) can significantly reduce the thermal motion rate of enzyme molecules and reduce their interaction with denaturing factors (such as metal ions and shear force).

[0056] For example, when extracting heat-sensitive proteases, if the centrifugation temperature exceeds 20°C, the enzyme activity may be lost by more than 50%, while a low temperature environment can increase the activity retention rate to more than 80%.

[0057] Low temperature can inhibit the reproduction of residual microorganisms in the raw materials, prevent the proteases or nucleases secreted by them from degrading the target enzyme, and at the same time reduce the activity of endogenous enzymes (such as tissue autolytic enzymes) in the raw materials to prevent the enzyme solution from deteriorating.

[0058] Process advantages of low-temperature centrifugation (Table 1)

[0059]

[0060]

[0061] In this embodiment, the concentration tank 5 is fixedly mounted on the frame 4 ; a controller 11 is also fixed on one side of the frame 4 .

[0062] The controller 11 is used to control various valves, booster pumps, delivery pumps and other electrical components, thereby achieving collaboration in the enzyme preparation extraction process.

[0063] In this embodiment, the concentration tank 5 is a sandwich tank with a heating coil provided in the sandwich layer; a stirring device is provided inside the concentration tank 5, and a pressure gauge, a pressure relief valve, a safety valve and a sampling port are installed on the tank top cover.

[0064] By employing this technical solution, the enzyme solution (such as fermentation supernatant or crushing supernatant) after cryocentrifugation is typically low in concentration (enzyme protein content may be only 0.1-1g / L). Direct purification would be inefficient due to its large volume. Using a concentrator (such as a vacuum concentrator, thin-film evaporator, or ultrafiltration membrane device) can reduce the volume of the enzyme solution to 1 / 5-1 / 10 of its original volume, increasing the enzyme concentration by 5-10 times (e.g., to 5-10g / L).

[0065] In α-amylase extraction, the enzyme activity units (U / mL) of the concentrated enzyme solution can be increased from 1000U to 5000-10000U, significantly reducing the processing capacity of subsequent chromatography columns. The increase in enzyme concentration during the concentration process may cause impurity proteins (such as those with properties significantly different from the target enzyme) to preferentially precipitate due to supersaturation, which can be further removed by subsequent centrifugation or filtration.

[0066] In this embodiment, an annular groove is formed on the outer edge of the sliding plate 241 , and an O-shaped rubber ring is embedded in the annular groove and fixed by glue.

[0067] By adopting the above technical solution, the sliding plate 241 strengthens the airtightness between the sliding plate 241 and the filter tank body 23 through the outer O-ring. After the compressed gas is introduced, the sliding plate 241 can slide up or down along the filter tank body 23.

[0068] The working principle of the present invention is as follows: after the biomaterial is crushed, the electromagnet 2412 and the metal block 2411 are attracted, the cross push rod 249 compresses the spring 2410, and lifts the elastic membrane 246, and the slider 243 moves along the cross slot 242 toward the axis of the sliding disk 241. At this time, the metal ring 245 is separated from the sliding disk 241, and a cavity is formed between the metal ring 245 and the sliding disk 241 to facilitate the entry of the enzyme solution into the filter membrane 25;

[0069] The elastic membrane 246 is lifted up in a conical shape, and the crushed biological material flows around along the conical elastic membrane 246 and then enters the filter membrane 25 between the metal ring 245 and the sliding disk 241. Then, the electromagnet 2412 loses power, and the cross rod 249 moves downward under the weight of the metal block 2411, and the filter membrane 25 returns to its original shape, closing between the metal ring 245 and the sliding disk 241.

[0070] Subsequently, compressed gas is introduced into the first air inlet pipe 28 (the second air inlet pipe is closed). As the air pressure in the crushing tank 21 continues to increase, the sliding plate 241 slides downward under the push of the air pressure and compresses the rubber bellows 26 and the filter membrane 25. The biological material in the filter membrane 25 is filtered by the filter membrane 25 and then enters the gap between the rubber bellows 26 and the filter membrane 25, and is output through the delivery pump 6.

[0071] When the biological material is filtered, there is not only mechanical pressure from the sliding plate 241, but also pressure from the compressed air inside the filter tank 23, which squeezes the biological material in the filter membrane 25 from all directions, thereby improving the effect of the biological material filtering the enzyme solution.

[0072] When the next round of biomaterial crushing is carried out, the first air inlet pipe 28 stops supplying air and the second air inlet pipe 29 starts supplying air. The compressed gas pushes the sliding plate 241 to slide upward along the filter tank body 23. When the sliding plate 241 contacts the raised ring inside the filter tank body 23, it stops moving. At this time, the sliding plate 241 and the protective shell 247 are in a closed state, and biomaterial can be added to the crushing tank body 21 for the next crushing process.

[0073] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the invention is defined by the appended claims, not the foregoing description, and all variations within the meaning and range of equivalents of the claims are intended to be included therein. Any reference sign in a claim should not be construed as limiting the claim to which it relates.

[0074] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. An enzyme preparation extraction device, characterized in that: The invention comprises a crushing and separating mechanism (2) mounted on a support body (1); the crushing and separating mechanism (2) comprises a crushing tank body (21), a crusher (22) being mounted on the top of the crushing tank body (21); the crushing tank body (21) is fixed on the top of a filtering tank body (23); an on-off assembly (24) is provided inside the filtering tank body (23); The on-off assembly (24) has a sliding plate (241) that slides up and down along the inner wall of the filter tank body (23); a cross groove (242) is provided on the upper surface of the sliding plate (241); a slider (243) is slidably connected in the cross groove (242); the slider (243) is movably connected to the movable arm (244) through a rotating shaft; the upper end of the movable arm (244) is movably connected to the metal ring (245) through a rotating shaft; an elastic membrane (246) is fixed in the middle position of the metal ring (245); and a protective shell (247) is welded to the outer ring of the bottom of the metal ring (245); A focusing hood (248) is fixed to the bottom of the sliding plate (241); a filter membrane (25) is fixed to the bottom of the focusing hood (248) through a flange, and a rubber bellows (26) is sleeved on the outside of the filter membrane (25); the bottoms of the rubber bellows (26) and the filter membrane (25) are both sealed and fixed to the support member (27); a liquid outlet pipe connected to the liquid inlet end of the delivery pump (6) is fixed to one side of the lower end of the rubber bellows (26) through a flange.

2. The enzyme preparation extraction device according to claim 1, characterized in that: The top of the crushing tank (21) is also provided with a first air inlet pipe (28); the bottom of the filtering tank (23) is provided with a second air inlet pipe (29).

3. The enzyme preparation extraction device according to claim 1, characterized in that: A cross bracket (2413) is welded inside the condenser (248), and a cross top rod (249) is slidably connected in the middle of the cross bracket (2413), and a spring (2410) is sleeved on the outside of the cross top rod (249); the top of the cross top rod (249) is threadedly connected to a screw sleeve bonded and arranged in the middle of the elastic membrane (246); the lower end of the cross top rod (249) passes through the bottom of the support member (27) and is slidably connected.

4. The enzyme preparation extraction device according to claim 3, characterized in that: A metal block (2411) is also fixed to the lower end of the cross top rod (249); and an electromagnet (2412) for attracting the metal block (2411) is also installed at the middle position of the bottom of the support member (27).

5. The enzyme preparation extraction device according to claim 1, characterized in that: The liquid outlet end of the delivery pump (6) is connected to an enzyme liquid delivery pipe (7), and the upper end of the enzyme liquid delivery pipe (7) is connected to a low-temperature centrifuge (3); the low-temperature centrifuge (3) is connected to a concentration tank (5) through a delivery pipe (9); and a booster pump (10) is also connected in series to the delivery pipe (9).

6. The enzyme preparation extraction device according to claim 5, characterized in that: The low-temperature centrifuge (3) has a casing with a sandwich layer, and the sandwich layer of the casing is connected to a cooling pipe (8); the cooling pipe (8) is connected to an air cooling or water cooling device. A rotating motor is fixed on the top of the casing, and the shaft of the rotating motor passes through the inside of the casing and is connected to the centrifugal screen; the enzyme liquid delivery pipe (7) is located above the centrifugal screen.

7. The enzyme preparation extraction device according to claim 5, characterized in that: The concentration tank (5) is fixedly mounted on the frame (4); a controller (11) is also fixed on one side of the frame (4).

8. The enzyme preparation extraction device according to claim 1, characterized in that: The concentration tank (5) is a sandwich tank with a heating coil inside the sandwich; a stirring device is provided inside the concentration tank (5), and a pressure gauge, a pressure relief valve, a safety valve and a sampling port are installed on the tank top cover.

9. The enzyme preparation extraction device according to claim 4, characterized in that: When the electromagnet (2412) and the metal block (2411) are attracted, the cross push rod (249) compresses the spring (2410) and lifts the elastic membrane (246), and the slider (243) moves along the cross slot (242) toward the axis of the sliding disk (241). At this time, the metal ring (245) is separated from the sliding disk (241), and a cavity is provided between the metal ring (245) and the sliding disk (241) to facilitate the enzyme liquid to enter the filter membrane (25); When the electromagnet (2412) and the metal block (2411) are separated, the spring (2410) pushes the cross rod (249) downward, the elastic membrane (246) is reset, the metal ring (245) and the sliding plate (241) are closed, and the flow of the enzyme liquid is cut off. At this time, the enzyme liquid is stored in the crushing tank (21) and crushed by the crusher (22).

10. The enzyme preparation extraction device according to claim 9, characterized in that: An annular groove is provided on the outer edge of the sliding plate (241), and an O-shaped rubber ring is embedded in the annular groove and fixed by glue.

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