Centrifugal micro-fluidic chip for rapid nucleic acid PCR amplification and fluorescence detection

Abstract

The invention belongs to the technical field of micro-fluidic chips, and particularly relates to a centrifugal micro-fluidic chip for rapid nucleic acid PCR amplification and fluorescence detection, the centrifugal micro-fluidic chip comprises a bottom plate, a micro-fluidic chip body and a connecting mechanism, the bottom plate is connected with the bottom of the micro-fluidic chip body through the connecting mechanism; a plurality of fastening blocks are fixedly connected to the edge of the bottom plate, fastening openings are formed in the fastening blocks, and a supporting column is fixedly connected to the top of the bottom plate; the connecting mechanism comprises a mounting block, a supporting groove corresponding to the supporting column is formed in the bottom of the mounting block, a plurality of clamping structures are slidably inserted into the supporting groove, a plurality of clamping grooves corresponding to the clamping structures are formed in the supporting column, a mounting groove is formed in the top of the mounting block, and an adjusting mechanism is rotationally connected into the mounting groove. Through the arrangement of the adjustable centrifugal mounting assembly, the micro-fluidic chip can be quickly connected with the centrifugal mechanism, so that the use efficiency of the micro-fluidic chip is effectively improved.

Description

Technical Field

[0001] The present invention relates to the technical field of microfluidic chips, in particular to a centrifugal microfluidic chip for rapid nucleic acid PCR amplification and fluorescence detection. Background Art

[0002] Microfluidics is a technology that uses microchannels and microcavities to control microfluidics and perform biochemical reactions. It is currently widely used in research related to tissues, cells, nucleic acids, proteins, and small molecules. Currently, the detection of multiple nucleic acid targets often requires multiplex amplification technology.

[0003] Nucleic acid testing, a key component of molecular diagnostics, can provide accurate diagnostic evidence for pathogen detection. Existing centrifugal microfluidic chips consist of a sample loading chamber, a reagent chamber, a detection chamber, and a waste liquid chamber. During testing, a sample is added to the sample loading chamber. Centrifugal force then drives the sample through the various reagent chambers for release, amplification, and mixing. Testing then occurs within the detection chamber, with the waste liquid ultimately discharged into the waste liquid chamber.

[0004] However, the external structure of the existing microfluidic chip is relatively simple, and the outside of the microfluidic chip lacks certain centrifugal mounting components, and cannot be quickly connected to the centrifugal mechanism, thereby affecting the use efficiency of the microfluidic chip. Summary of the Invention

[0005] 1. Technical problems to be solved

[0006] The purpose of the present invention is to solve the problem in the prior art that the external part of the microfluidic chip lacks certain centrifugal mounting components and cannot be quickly connected to the centrifugal mechanism, and to propose a centrifugal microfluidic chip for rapid nucleic acid PCR amplification and fluorescence detection.

[0007] 2. Technical solution

[0008] In order to achieve the above object, the present invention adopts the following technical solutions:

[0009] A centrifugal microfluidic chip for rapid nucleic acid PCR amplification and fluorescence detection, comprising a bottom plate, a microfluidic chip body, and a connecting mechanism, wherein the bottom plate is connected to the bottom of the microfluidic chip body via the connecting mechanism;

[0010] The edges of the bottom plate are fixedly connected with a plurality of fastening blocks, each of which is provided with a fastening opening, and the top of the bottom plate is fixedly connected with a support column;

[0011] The connecting mechanism includes a mounting block, a supporting groove corresponding to the supporting column is provided at the bottom of the mounting block, a plurality of clamping structures are slidably inserted in the supporting groove, a plurality of clamping grooves corresponding to the clamping structures are provided on the supporting column, a mounting groove is provided at the top of the mounting block, and an adjustment mechanism is rotatably connected in the mounting groove;

[0012] A connecting plate is fixedly connected to the bottom of the microfluidic chip body, and the connecting plate is connected to the adjustment mechanism through two symmetrically arranged connecting rods. The two ends of the connecting rod are respectively rotatably connected to the connecting plate and the adjustment mechanism. One end of the connecting plate is fixedly connected to a guide rod, and the top of the mounting block is provided with a guide groove corresponding to the guide rod.

[0013] Preferably, a threaded connection groove is provided at the bottom center of the bottom plate.

[0014] Preferably, a plurality of symmetrically arranged positioning rods are fixedly connected to the bottom of the mounting block, and a plurality of positioning holes corresponding to the positioning rods are provided on the top of the base plate.

[0015] Preferably, the clamping structure includes a clamping rod, an end of the clamping rod close to the support column is fixedly sleeved with a clamping sleeve, and an end of the clamping rod away from the clamping sleeve is fixedly connected to a pull rod.

[0016] Preferably, a first spring is sleeved on the clamping rod, and two ends of the first spring are fixedly connected to the clamping sleeve and the inner wall of the supporting groove respectively.

[0017] Preferably, the inner top of the support groove is fixedly connected to a limiting block, and the top of the support column is provided with a limiting groove corresponding to the limiting block.

[0018] Preferably, the adjusting mechanism includes a screw, a transmission block is threadedly sleeved on the screw, one end of the mounting block is provided with a cavity corresponding to the screw, a worm is rotatably inserted in the cavity, a worm wheel meshing with the worm is fixedly sleeved on the screw, one end of the worm passes through the inner wall of the cavity and is fixedly connected to a knob, a locking groove is provided on the outer wall of the mounting block, and a locking structure corresponding to the knob is fixedly connected in the locking groove.

[0019] Preferably, the locking structure includes a vertical rod, an L-shaped locking rod is slidably sleeved on the vertical rod, and a plurality of locking holes corresponding to the L-shaped locking rod are arranged around the knob.

[0020] Preferably, a second spring is sleeved on the vertical rod, and two ends of the second spring respectively abut against the L-shaped locking rod and the inner wall of the locking groove.

[0021] 3. Beneficial effects

[0022] Compared with the prior art, the advantages of the present invention are:

[0023] (1) In the present invention, the microfluidic chip can be quickly connected to the centrifugal mechanism by setting up an adjustable centrifugal mounting assembly, thereby effectively improving the utilization efficiency of the microfluidic chip.

[0024] (2) In the present invention, the setting of the threaded connection groove can facilitate connection with the external centrifugal mechanism. At the same time, the setting of the positioning rod can prevent rotation between the mounting block and the base plate. The setting of the clamping structure can effectively install and fix the support column. At the same time, the clamping sleeve can prevent the clamping rod from falling out of the support groove. The first spring can play a certain elastic supporting role on the clamping rod.

[0025] (3) In the present invention, the setting of the limit block can prevent the support column from shaking, and the setting of the locking mechanism can effectively fix the knob. The setting of the guide rod can prevent the microfluidic chip body from shaking, and the second spring can play a certain elastic supporting role on the L-shaped locking rod. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a schematic structural diagram of a centrifugal microfluidic chip for rapid nucleic acid PCR amplification and fluorescence detection proposed by the present invention;

[0027] Figure 2 This is a schematic diagram of the top view of the bottom plate of a centrifugal microfluidic chip for rapid nucleic acid PCR amplification and fluorescence detection proposed by the present invention;

[0028] Figure 3 This is a schematic diagram of the top view of the screw of a centrifugal microfluidic chip for rapid nucleic acid PCR amplification and fluorescence detection proposed by the present invention;

[0029] Figure 4 This is a side view structural diagram of the worm of a centrifugal microfluidic chip for rapid nucleic acid PCR amplification and fluorescence detection proposed by the present invention.

[0030] In the figure: 1 bottom plate, 2 microfluidic chip body, 3 fastening block, 4 support column, 5 mounting block, 6 mounting slot, 7 connecting plate, 8 connecting rod, 9 guide rod, 10 threaded connection slot, 11 positioning rod, 12 clamping rod, 13 clamping sleeve, 14 pull rod, 15 first spring, 16 limit block, 17 screw, 18 transmission block, 19 worm, 20 worm gear, 21 knob, 22 vertical rod, 23 L-shaped locking rod, 24 second spring. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0032] Example 1:

[0033] Reference Figure 1-4 A centrifugal microfluidic chip for rapid nucleic acid PCR amplification and fluorescence detection includes a base plate 1, a microfluidic chip body 2, and a connecting mechanism. The base plate 1 is connected to the bottom of the microfluidic chip body 2 by the connecting mechanism. A threaded connection groove 10 is provided at the center of the bottom of the base plate 1 to facilitate connection with an external centrifugal drive mechanism.

[0034] In the present invention, a plurality of fastening blocks 3 are fixedly connected to the edge of the bottom plate 1. The fastening blocks 3 are provided with fastening openings for convenient connection with an external centrifugal drive mechanism. A support column 4 is fixedly connected to the top of the bottom plate 1 for connection with the mounting block 5.

[0035] In the present invention, the connection mechanism includes a mounting block 5, the bottom of the mounting block 5 is fixedly connected to a plurality of symmetrically arranged positioning rods 11, the top of the base plate 1 is provided with a plurality of positioning holes corresponding to the positioning rods 11, which can prevent the mounting block 5 from shaking, the bottom of the mounting block 5 is provided with a support groove corresponding to the support column 4, the inner top of the support groove is fixedly connected to a limit block 16, which can prevent the support column 4 from shaking, and the top of the support column 4 is provided with a limit groove corresponding to the limit block 16;

[0036] In the present invention, a plurality of clamping structures are slidably inserted in the supporting groove, and a plurality of clamping grooves corresponding to the clamping structures are provided on the supporting column 4. The clamping structure includes a clamping rod 12 for clamping and fixing the supporting column 4. One end of the clamping rod 12 close to the supporting column 4 is fixedly sleeved with a clamping sleeve 13, and the end of the clamping rod 12 away from the clamping sleeve 13 is fixedly connected with a pull rod 14 for convenient pulling of the clamping rod 12. A first spring 15 is sleeved on the clamping rod 12, and the two ends of the first spring 15 are respectively fixedly connected to the clamping sleeve 13 and the inner wall of the supporting groove, which provides a certain elastic support for the clamping rod 12. A mounting groove 6 is provided on the top of the mounting block 5, and an adjustment mechanism is rotatably connected in the mounting groove 6 to adjust the height of the microfluidic chip body 2;

[0037] In the present invention, the adjustment mechanism includes a screw 17, which is used to drive a transmission block 18. The transmission block 18 is threadedly sleeved on the screw 17. One end of the mounting block 5 is provided with a cavity corresponding to the screw 17. A worm 19 is rotatably inserted in the cavity to drive a worm gear 20 to rotate. A worm gear 20 meshing with the worm 19 is fixedly sleeved on the screw 17 to drive the screw 17 to rotate. One end of the worm 19 passes through the inner wall of the cavity and is fixedly connected to a knob 21 to facilitate the rotation of the worm 19. A locking groove is provided on the outer wall of the mounting block 5, and a locking structure corresponding to the knob 21 is fixedly connected in the locking groove to lock and fix the knob 21.

[0038] In the present invention, the locking structure includes a vertical rod 22, on which an L-shaped locking rod 23 is slidably sleeved. A plurality of locking holes corresponding to the L-shaped locking rod 23 are arranged around the knob 21. A second spring 24 is sleeved on the vertical rod 22. The two ends of the second spring 24 respectively abut against the L-shaped locking rod 23 and the inner wall of the locking groove, providing a certain elastic support for the L-shaped locking rod 23.

[0039] In the present invention, a connecting plate 7 is fixedly connected to the bottom of the microfluidic chip body 2, and the connecting plate 7 is connected to the adjustment mechanism through two symmetrically arranged connecting rods 8. The two ends of the connecting rod 8 are rotatably connected to the connecting plate 7 and the adjustment mechanism respectively. One end of the connecting plate 7 is fixedly connected to a guide rod 9, and the top of the mounting block 5 is provided with a guide groove corresponding to the guide rod 9, which is used to prevent the microfluidic chip body 2 from shaking.

[0040] In the present invention, the base plate 1 is first connected to the external traction mechanism through the fastening block 3, and then the support column 4 is inserted into the support groove, and then the support column 4 is fixed by the clamping mechanism to realize the connection between the support column 4 and the base plate 1. At the same time, the first spring 15 can play a certain elastic traction role on the clamping rod 12, and then the knob 21 is rotated to drive the worm 19 to rotate, and through the meshing action between the worm 19 and the worm wheel 20, the screw 17 is driven to rotate, and the transmission action of the transmission block 18 and the connecting rod 8 is used to drive the microfluidic chip body 2 to move, and adjust the height of the microfluidic chip body 2. Then the locking mechanism is used to fix the knob 21. At the same time, the setting of the guide rod 9 can prevent the microfluidic chip body 2 from shaking.

[0041] In the present invention, the microfluidic chip can be quickly connected to the centrifugal mechanism by providing an adjustable centrifugal mounting assembly, thereby effectively improving the utilization efficiency of the microfluidic chip.

[0042] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. A centrifugal microfluidic chip for rapid nucleic acid PCR amplification and fluorescence detection, comprising a base plate (1), a microfluidic chip body (2) and a connecting mechanism, characterized in that: The bottom plate (1) is connected to the bottom of the microfluidic chip body (2) via a connecting mechanism; A plurality of fastening blocks (3) are fixedly connected to the edge of the bottom plate (1), and fastening openings are provided on the fastening blocks (3). A support column (4) is fixedly connected to the top of the bottom plate (1); The connecting mechanism comprises a mounting block (5), the bottom of the mounting block (5) is provided with a support groove corresponding to the support column (4), a plurality of clamping structures are slidably inserted in the support groove, the support column (4) is provided with a plurality of clamping grooves corresponding to the clamping structures, the top of the mounting block (5) is provided with a mounting groove (6), and an adjustment mechanism is rotatably connected in the mounting groove (6); The bottom of the microfluidic chip body (2) is fixedly connected to a connecting plate (7), and the connecting plate (7) is connected to the adjustment mechanism through two symmetrically arranged connecting rods (8). The two ends of the connecting rod (8) are rotatably connected to the connecting plate (7) and the adjustment mechanism respectively. One end of the connecting plate (7) is fixedly connected to a guide rod (9), and the top of the mounting block (5) is provided with a guide groove corresponding to the guide rod (9).

2. A centrifugal microfluidic chip for rapid nucleic acid PCR amplification and fluorescence detection according to claim 1, characterized in that: A threaded connection groove (10) is provided at the bottom center of the base plate (1).

3. A centrifugal microfluidic chip for rapid nucleic acid PCR amplification and fluorescence detection according to claim 1, characterized in that: The bottom of the mounting block (5) is fixedly connected to a plurality of symmetrically arranged positioning rods (11), and the top of the base plate (1) is provided with a plurality of positioning holes corresponding to the positioning rods (11).

4. A centrifugal microfluidic chip for rapid nucleic acid PCR amplification and fluorescence detection according to claim 1, characterized in that: The clamping structure comprises a clamping rod (12), one end of the clamping rod (12) close to the support column (4) is fixedly sleeved with a clamping sleeve (13), and one end of the clamping rod (12) away from the clamping sleeve (13) is fixedly connected with a pull rod (14).

5. A centrifugal microfluidic chip for rapid nucleic acid PCR amplification and fluorescence detection according to claim 4, characterized in that: A first spring (15) is sleeved on the clamping rod (12), and two ends of the first spring (15) are fixedly connected to the clamping sleeve (13) and the inner wall of the support groove respectively.

6. A centrifugal microfluidic chip for rapid nucleic acid PCR amplification and fluorescence detection according to claim 1, characterized in that: The inner top of the support groove is fixedly connected to a limiting block (16), and the top of the support column (4) is provided with a limiting groove corresponding to the limiting block (16).

7. A centrifugal microfluidic chip for rapid nucleic acid PCR amplification and fluorescence detection according to claim 1, characterized in that: The adjusting mechanism comprises a screw (17), a transmission block (18) is threadedly sleeved on the screw (17), one end of the mounting block (5) is provided with a cavity corresponding to the screw (17), a worm (19) is rotatably inserted in the cavity, a worm wheel (20) is fixedly sleeved on the screw (17) and meshed with the worm (19), one end of the worm (19) passes through the inner wall of the cavity and is fixedly connected to a knob (21), a locking groove is provided on the outer wall of the mounting block (5), and a locking structure corresponding to the knob (21) is fixedly connected in the locking groove.

8. A centrifugal microfluidic chip for rapid nucleic acid PCR amplification and fluorescence detection according to claim 7, characterized in that: The locking structure comprises a vertical rod (22), an L-shaped locking rod (23) is slidably sleeved on the vertical rod (22), and a plurality of locking holes corresponding to the L-shaped locking rod (23) are arranged around the knob (21).

9. The centrifugal microfluidic chip for rapid nucleic acid PCR amplification and fluorescence detection according to claim 8, characterized in that: A second spring (24) is sleeved on the vertical rod (22), and two ends of the second spring (24) respectively abut against the L-shaped locking rod (23) and the inner wall of the locking groove.

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