Stenotrophomonas maltophilia strain capable of degrading penthiopyrad and application of stenotrophomonas maltophilia strain
By screening the Stenotrophomonas maltophilia strain PEB1, the problem of low degradation efficiency of penthiopyrad in soil was solved, efficient and rapid degradation of penthiopyrad was achieved, environmental risks were reduced, and it is suitable for bioremediation of pesticide pollution.
Patent Information
- Application Number
- CN202510865064.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-06-26
- Publication Date
- 2025-09-26
AI Technical Summary
In the existing technology, the degradation efficiency of penthiopyrad in soil is low, there are risks of persistent residues and ecotoxicity, and there is a lack of efficient microbial degradation methods.
A strain of Stenotrophomonas maltophilia, PEB1, was screened out. This strain can efficiently degrade penthiopyrad in a wide temperature range and exhibits good degradation performance in pesticide-contaminated environments. It is suitable for pesticide production wastewater treatment, contaminated soil bioremediation and surface water treatment.
The strain PEB1 of Stenotrophomonas maltophilia can achieve a 90% degradation rate of penthiopyrad within 7 days, and the degradation rate in soil can reach 60.4%, significantly improving the degradation efficiency of penthiopyrad, shortening its half-life in the soil, and reducing environmental risks.
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of microbial degradation of penthiopyrad in soil, and particularly relates to a strain of Stenotrophomonas maltophilia capable of degrading penthiopyrad and application thereof. Background Art
[0002] Penthiopyrad, a representative example of the succinate dehydrogenase inhibitor fungicide class, has been registered and commercialized in 35 major agricultural countries worldwide (covering Asia, North America, and the European Union). Its target is highly consistent with that of SDHI compounds. It specifically binds to the succinate dehydrogenase complex in the inner mitochondrial membrane of pathogens, interfering with the electron transport chain, blocking the tricarboxylic acid cycle and hindering energy metabolism, ultimately leading to the pathogen's death. This compound exhibits broad-spectrum and highly effective fungicidal properties and is widely used to control diseases such as rust, gray mold, head blight, and leaf mold in crops such as fruit trees, vegetables, and turfgrass.
[0003] Penthiopyrad has attracted widespread attention due to its high persistence in soil environments. Existing data indicate that the compound has a half-life of 65-356 days in various soil types. This persistent residue not only significantly increases the risk of accumulation through the food chain but also poses a potential threat to ecosystem stability and human health. Studies on its ecotoxicological effects have shown that exposure to penthiopyrad during the critical period of embryonic and larval development in zebrafish can cause dose-dependent acute toxic effects, manifested by developmental abnormalities such as decreased hatching rate, segmental malformations, and motor dysfunction. Further mechanistic studies have revealed that the compound may interfere with the activity of key enzymes in the melanin synthesis pathway, leading to abnormal differentiation of larval pigment cells, manifested as disrupted melanin deposition patterns and abnormalities in the synthesis pathway. Given the combined effects of penthiopyrad's persistence in environmental media and its significant developmental toxicity, the development of efficient and environmentally friendly pollution remediation technologies has become a pressing research area in environmental science and ecotoxicology.
[0004] Compared to traditional physical and chemical remediation technologies, which suffer from high energy consumption and secondary pollution, microbial degradation offers advantages such as high degradation efficiency and zero secondary pollution, making it a recognized environmentally friendly pesticide degradation method. Currently, there are few reports on the microbial degradation of penthiopyrad. When evaluating the effects of Trichoderma and Bacillus subtilis on penthiopyrad concentration, Podbielska et al. found that Bacillus subtilis had a degradation efficiency of approximately 5% for penthiopyrad over a 14-day experimental period. For penthiopyrad treated with Trichoderma, the degradation efficiency was 34.2% on the third day and reached 56.9% on the 14th day. In mixed culture experiments, the degradation efficiency of penthiopyrad was 23.7% on the third day and 29.1% on the 14th day. Dou Li isolated six dominant degrading bacterial strains from contaminated soil that use penthiopyrad as their sole carbon source: Pseudomonas sp., Paenarthrobacter nicotinovorans, Acinetobacter sp., Serratia marcescens, Pandorae sp., and Burkholderia sp. These six strains demonstrated the ability to degrade penthiopyrad in an inorganic salt culture medium, with degradation ranging from 60% to 75%. Furthermore, they found that the pH, salinity, and temperature of the culture environment could influence the biodegradation of the degrading bacteria by altering their growth. In soil remediation experiments, the presence of all six strains promoted the degradation of penthiopyrad in soil, shortening its half-life by 2.6 to 30.4 days. However, these studies suffer from a drawback: low penthiopyrad degradation efficiency. Currently, there are no patent reports on microbial degradation of penthiopyrad residues.
[0005] Stenotrophomonas maltophilia is a Gram-negative bacterium belonging to the Proteobacteria, subclass γ-β, genus Stenotrophomonas. It is widely distributed in the environment and has been found in soil, water, and on animal surfaces. It has shown potential for pesticide degradation, biocontrol, and plant growth promotion. However, its ability to degrade penthiopyrad has not been reported. The present invention discloses a penthiopyrad-degrading bacterium and its application. The present invention discovered for the first time the degradation effect of Stenotrophomonas maltophilia on penthiopyrad, and screened out a strain of Stenotrophomonas maltophilia PEB1 that can efficiently degrade penthiopyrad. Based on the present invention, Stenotrophomonas maltophilia has a significant biodegradation effect on penthiopyrad, and can effectively degrade it within a wide temperature range and tolerate higher concentrations of penthiopyrad. The strain can be effectively applied to environmental remediation scenarios such as pesticide production wastewater treatment, bioremediation of contaminated soil, and in-situ treatment of surface water bodies. The present invention provides a new and efficient microbial resource for the treatment of organic pesticide pollution. Its strong environmental adaptability and high operational feasibility have opened up a new path for the development of green bioremediation technology. Summary of the Invention
[0006] The technical problem solved by the present invention is to provide a strain of Stenotrophomonas maltophilia, which can efficiently degrade penthiopyrad and can be used to prepare a bioremediation agent for agricultural land contaminated by the pesticide penthiopyrad, and has good application prospects in soil remediation treatment.
[0007] The purpose of the present invention is to provide an application of Stenotrophomonas maltophilia in degrading and repairing a natural environment contaminated by penthiopyrad.
[0008] Another object of the present invention is to provide a strain of Stenotrophomonas maltophilia PEB1 that can efficiently degrade penthiopyrad.
[0009] Another object of the present invention is to provide a use of the Stenotrophomonas maltophilia strain PEB1 in degrading and repairing a natural environment contaminated by penthiopyrad.
[0010] The above-mentioned purpose of the present invention is achieved through the following technical solutions:
[0011] The present invention discovered for the first time the degradation effect of Stenotrophomonas maltophilia on penthiopyrad, and screened out a Stenotrophomonas maltophilia strain PEB1 that is efficient and fast in degrading penthiopyrad. The strain was deposited in the General Microbiology Center of the China Culture Collection Administration on December 24, 2024, with the deposit number: CGMCC No. 33180.
[0012] This strain (PEB1) was isolated from farmland soil where penthiopyrad was applied for a long time, and was obtained through artificial enrichment culture and multiple generations of purification. Experiments have shown that it has significant degradation characteristics: in an inorganic salt culture medium with penthiopyrad as the sole carbon source, the degradation rate exceeded 90% in 7 days; it has both adaptability to a wide temperature range and tolerance to high-concentration pesticides. Soil remediation experiments showed that 60 days after the addition of the strain, the degradation rate of penthiopyrad in sterilized soil reached 52.3% (an increase of 6.0% compared to 46.5% in the blank control group); and in a non-sterile system with competition from indigenous microorganisms, the degradation efficiency was further increased to 60.4% (an increase of 8.7% compared to 51.7% in the control group), confirming its ecological competitive advantage. This strain can efficiently degrade penthiopyrad residues in the environment, and has shown important application value in the field of bioremediation of pesticide pollution.
[0013] Therefore, the following applications should all be within the scope of protection of the present invention:
[0014] Application of Stenotrophomonas maltophilia in degrading penthiopyrad or preparing degradation agents.
[0015] Application of Stenotrophomonas maltophilia in remediating natural environments contaminated by penthiopyrad or preparing remediation agents.
[0016] The application of the Stenotrophomonas maltophilia strain PEB1 in degrading penthiopyrad or preparing a degradation bacterial agent.
[0017] The application of the Stenotrophomonas maltophilia strain PEB1 in repairing a natural environment contaminated by penthiopyrad or preparing a repair bacterial agent.
[0018] The natural environment includes water bodies or soil, etc.
[0019] Preferably, the Stenotrophomonas maltophilia is the Stenotrophomonas maltophilia strain PEB1.
[0020] The present invention discloses for the first time the degradation effect of Stenotrophomonas maltophilia on penthiopyrad, and screens out a Stenotrophomonas maltophilia strain PEB1 that can efficiently degrade penthiopyrad, providing a new and efficient microbial resource for the treatment of organic pesticide pollution. The microbial resource has strong environmental adaptability and high operational feasibility, opening up a new path for the development of green bioremediation technology.
[0021] Moreover, the bacteria can effectively degrade penthiopyrad in a wide temperature range and tolerate a higher concentration of penthiopyrad (250 mg / L), proving that strain PEB1 can be used as an excellent pesticide-degrading bacterium in the bioremediation of penthiopyrad-contaminated environments. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] Figure 1 This is a diagram showing the colony morphology characteristics of the Stenotrophomonas maltophilia strain PEB1 cultured on LB solid medium for 2 days.
[0023] Figure 2 These are scanning electron micrographs and Gram staining images of Stenotrophomonas maltophilia strain PEB1.
[0024] Figure 3 is the phylogenetic tree of 16S rDNA of Stenotrophomonas maltophilia strain PEB1.
[0025] Figure 4 is the growth curve of Stenotrophomonas maltophilia strain PEB1.
[0026] Figure 5 is the degradation curve of Stenotrophomonas maltophilia strain PEB1.
[0027] Figure 6 The degradation effect of Stenotrophomonas maltophilia strain PEB1 on penthiopyrad under different temperature conditions.
[0028] Figure 7 Degradation effect of Stenotrophomonas maltophilia strain PEB1 on penthiopyrad under different initial concentrations.
[0029] Figure 8 Degradation effect of Stenotrophomonas maltophilia strain PEB1 on penthiopyrad under different inoculum conditions.
[0030] Figure 9 The effect of Stenotrophomonas maltophilia strain PEB1 on the degradation of penthiopyrad in sterilized soil.
[0031] Figure 10 Figure 3. Effect of Stenotrophomonas maltophilia strain PEB1 on the degradation of penthiopyrad in non-sterile soil. DETAILED DESCRIPTION
[0032] The above contents of the present invention are further described in detail below through examples, but this should not be understood as limiting the scope of the above subject matter of the present invention to the following examples. All technologies implemented based on the above contents of the present invention fall within the scope of the present invention.
[0033] Unless otherwise specified, the reagents and materials used in the following examples were commercially available.
[0034] The culture medium formulations in the following examples are as follows:
[0035] Mineral salts medium (MSM): 1 g ammonium nitrate, 0.2 g magnesium sulfate heptahydrate, 1 g sodium chloride, 1.5 g potassium dihydrogen phosphate, 0.5 g dipotassium hydrogen phosphate, and 1000 mL distilled water, pH 7.2. Solid medium: Add 18-20 g agar powder per 1 L of liquid medium.
[0036] LB medium: 10 g of tryptone, 5 g of yeast powder, 10 g of sodium chloride, and 1000 mL of distilled water, pH 7.2; solid medium: add 18-20 g of agar powder per 1 L of liquid medium.
[0037] Example 1 Isolation and identification of strains
[0038] 1. Screening and isolation of penthiopyrad-degrading strains:
[0039] Soil samples chronically contaminated with penthiopyrad were collected, air-dried in a cool, dry place, and sieved. A 5g sample was weighed and added to 50mL of sterile MSM medium containing 50mg / L penthiopyrad and incubated in a constant-temperature shaker (28°C, 160rpm). Every 7 days, the culture was transferred to an inorganic liquid medium containing increasing concentrations of penthiopyrad at a 5% inoculum size, starting at 50mg / L, 100mg / L, 200mg / L, 300mg / L, 400mg / L, and 500mg / L. The final culture was plated onto an inorganic solid medium containing 100mg / L penthiopyrad. After incubation at 28°C for 4-5 days, colonies were picked and streaked three times for purification. The purified strain was then plated onto plates containing 100mg / L penthiopyrad, resulting in the isolation of a highly efficient degrading bacterium designated PEB1.
[0040] 2. Identification of strain PEB1
[0041] (1) Morphological identification:
[0042] The degradation strain PEB1 was cultured on LB solid medium plates at 30°C for 24 hours and the colony morphology was observed. The colonies of strain PEB1 on LB solid medium were opaque, bright yellow, round, convex, with neat edges, moist surface and glossy ( Figure 1 ); Scanning electron microscopy results showed that PEB1 had no spores, and the bacteria were short rod-shaped with no other special structures ( Figure 2 )
[0043] (2) Physiological and biochemical identification:
[0044] Physiological and biochemical tests of strain PEB1 showed that the methyl red reaction, starch hydrolysis, indole, and VP tests were all negative, while the nitrate reduction and citrate tests were positive. The results of its physiological and biochemical identification are shown in Table 1.
[0045] Table 1 Physiological and biochemical characteristics of strain PEB1 Note: +: Positive; -: Negative.
[0046] (3) 16S rDNA molecular biological identification:
[0047] Genomic DNA from strain PEB1 was extracted and used as a template for PCR amplification using universal 16S rDNA bacterial primers (27F: 5'-AGAGTTTGATCCTGGCTCAG-3'; 1429R: 5'-GGTTACCTTGTTACGACTT-3'). The PCR product was sent to Sangon Biotech (Shanghai) Co., Ltd. for sequencing. The sequenced strain gene sequences were compared against the NCBI database using BLAST, and a phylogenetic tree was constructed using MEGA 11.0.
[0048] The phylogenetic tree of 16S rDNA is as follows Figure 3 As shown, strain PEB1 has the highest homology with Stenotrophomonas maltophilia (GU254017) and is very close to its taxonomic status. Its culture characteristics and scanning electron microscopic observation characteristics are also most similar to those of Stenotrophomonas maltophilia. Therefore, the degrading bacteria screened in the present invention were identified as Stenotrophomonas maltophilia.
[0049] Based on the above identification results, the strain PEB1 of the present invention was identified as Stenotrophomonas maltophilia, and was deposited in the General Microbiology Center of the China Culture Collection Administration of Microorganisms on December 24, 2024, with the deposit number: CGMCC No.33180.
[0050] Example 2 Experiment on the degradation effect of strain PEB1 on penthiopyrad
[0051] 1. Experimental methods
[0052] (1) Preparation of bacterial suspension: The degradative strain PEB1 obtained by screening was inoculated into LB medium and cultured under shaking conditions of 30°C and 160 rpm for 18 h. The bacteria were collected by centrifugation at 5000 r / min for 5 min, washed twice with sterile saline, and resuspended in inorganic salt liquid medium to prepare bacterial suspension (OD 600 =1), and conduct subsequent experiments.
[0053] (2) Growth curve determination: PEB1 bacterial solution was inoculated into sterile LB medium at a 2% inoculum volume. Samples were taken before culture as a control, and the growth rate of the strain at 0 h of culture was recorded. The culture was placed at 28°C and 150 rpm for 96 h. Samples were taken at 0 h, 2 h, 4 h, 6 h, 8 h, 10 h, 12 h, 14 h, 16 h, 18 h, 20 h, 22 h, 24 h, 36 h, 48 h, 60 h, 72 h, 84 h, and 96 h, and the OD of the samples at each period was measured.600 value.
[0054] (3) Degradation performance determination: The bacterial suspension of strain PEB1 was inoculated into 100 mL of inorganic salt liquid culture medium containing 100 mg / L penthiopyrad. The inorganic salt liquid culture medium without inoculation but only with drug was used as a blank control. Samples were taken at 0, 1, 2, 3, 4, 5, 6, and 7 days, and the degradation of penthiopyrad was determined by HPLC.
[0055] (4) Chromatographic conditions:
[0056] HPLC: Agilent 1260
[0057] Chromatographic column: DIKMA Leapsil-C18 (4.6mm×150mm×5μm)
[0058] Column temperature: 30°C;
[0059] Mobile phase: acetonitrile: water = 70:30 (V / V)
[0060] Flow rate: 1 mL / min
[0061] Injection volume: 20 μL
[0062] Detection wavelength: 230nm
[0063] The degradation rate of penthiopyrad was calculated according to the following formula: Degradation rate (%) = (C–C0) / C×100,
[0064] Wherein, C is the initial concentration of penthiopyrad (mg / L), and C0 is the residual concentration of penthiopyrad in the inorganic salt liquid culture medium after the culture is completed (mg / L).
[0065] Quality control: The external standard method was used to calibrate the standard substances and prepare the standard curve.
[0066] 2. Experimental results
[0067] The results are as follows Figure 4 and Figure 5 As shown, the overall growth trend is as follows: 0 to 4 hours is the adaptation period, 4 to 24 hours is the logarithmic growth period, and the bacterial mass increases rapidly. From 24 hours on, the stable period begins and lasts until 72 hours, after which bacterial growth begins to decrease and enters the decline period. Strain PEB1 can utilize penthiopyrad as a sole carbon source, and the degradation rate changes with incubation time. The residual rate of penthiopyrad gradually decreases with time and then levels off. After 5 days of incubation, the degradation rate reaches a maximum of over 80%, after which the degradation rate slows. On the 7th day, strain PEB1's degradation rate of penthiopyrad reaches 90%.
[0068] The results showed that strain PEB1 could use penthiopyrad as the sole carbon source for growth and reproduction. When the concentration of penthiopyrad was 100 mg / L, the degradation rate reached 90% after 7 days of cultivation, indicating that the strain had the ability to degrade penthiopyrad efficiently and quickly.
[0069] Example 3 Study on the degradation characteristics of strain PEB1 on penthiopyrad
[0070] 1. Experimental methods
[0071] (1) Effect of temperature on the degradation of penthiopyrad by PEB1:
[0072] A 3% inoculum of the bacterial suspension was inoculated into 50 mL of an inorganic salt liquid culture medium containing an initial concentration of 100 mg / L of penthiopyrad. The culture was shaken at 20°C, 25°C, 30°C, and 35°C at 160 rpm. Samples were collected after 7 days of incubation to determine the residual concentration of penthiopyrad.
[0073] (2) Effect of initial concentration of penthiopyrad on the degradation of penthiopyrad by PEB1:
[0074] A 3% inoculum of the bacterial suspension was inoculated into 50 mL of inorganic salt liquid culture medium containing initial concentrations of 10 mg / L, 20 mg / L, 50 mg / L, 100 mg / L, 150 mg / L, 200 mg / L, and 250 mg / L of penthiopyrad. The culture was shaken at 30°C and 160 rpm. Samples were collected after 7 days of incubation to determine the residual concentration of penthiopyrad.
[0075] (3) Effect of inoculum size on the degradation of penthiopyrad by PEB1:
[0076] The bacterial suspension was inoculated into 50 mL of inorganic salt liquid culture medium containing an initial concentration of 100 mg / L penthiopyrad at inoculum sizes of 1%, 3%, 5%, 10%, and 20%. The suspension was shaken at 30°C and 160 rpm. Samples were collected after 7 days of incubation to determine the residual concentration of penthiopyrad.
[0077] 2. Experimental results are as follows Figures 6-8 shown.
[0078] Figure 6 The study shows the effect of temperature on the degradation of penthiopyrad by strain PEB1. Within the temperature range of 20-35°C, the degradation efficiency of penthiopyrad by strain PEB1 was significantly independent of temperature. Notably, the degradation rate remained consistently above 85% (p>0.05) throughout the entire tested temperature range, demonstrating that strain PEB1 possesses excellent degradation properties over a wide temperature range.
[0079] Figure 7The results show the effect of initial penthiopyrad concentration on the degradation performance of strain PEB1. When the initial concentration of penthiopyrad ranged from 10 to 250 mg / L, the degradation rate of penthiopyrad initially increased and then decreased with increasing concentration. Strain PEB1 can utilize penthiopyrad as its sole carbon source and can tolerate concentrations up to 250 mg / L. At an initial concentration of 100 mg / L, strain PEB1 achieved a maximum degradation rate of 93.84%.
[0080] Figure 8 The results show the effect of inoculum size on the degradation of penthiopyrad by strain PEB1. As the inoculum size increases, the degradation rate of penthiopyrad by strain PEB1 also increases. Strain PEB1 achieves optimal degradation of penthiopyrad at an inoculum size of 3%. When the inoculum size increases from 5% to 20%, the degradation rate does not increase but instead shows a downward trend (p<0.05), although it still maintains a high degradation rate.
[0081] The results showed that the degradation of penthiopyrad by strain PEB1 was not greatly affected by the environment. It could efficiently and quickly degrade penthiopyrad under existing conventional conditions. For example, it could quickly degrade penthiopyrad within a wide temperature range (20-35°C) and could tolerate 250 mg / L penthiopyrad, which provided a guarantee for its application in complex environments.
[0082] Example 4 Study on the Degradation Effect of Strain PEB1 on Penthiopyrad in Soil
[0083] 1. Test soil sample
[0084] The test soil was taken from the experimental field in Xindong District of Henan University of Science and Technology. It was yellow loam soil and had not been treated with penthiopyrad for more than 5 years. Before use, the soil was removed from the soil and air-dried in a cool and ventilated place. The soil was then sieved through a 2.0 mm pore size sieve for later use.
[0085] Treatment 1 (Sterilized Soil): 300 g of treated soil sample was accurately weighed and placed in a 500 mL Erlenmeyer flask, sealed with breathable sealing film, and sterilized by high-temperature moist heat for 30 minutes. After drying at 30°C, an inorganic salt liquid medium containing penthiopyrad (final concentration of 50 mg / kg penthiopyrad) was added to the soil in a clean bench to bring the soil moisture content to 60% of field capacity, and the soil was mixed thoroughly. A suspension of degrading bacteria was added to the experimental group and mixed thoroughly with the soil. A separate soil sample without the addition of degrading bacteria at the same concentration served as a blank control. Samples were collected at 0 (2 hours), 1, 3, 5, 7, 14, 28, 36, 45, and 60 days, and the residual penthiopyrad was determined and the degradation rate calculated. Throughout the experiment, water was continuously replenished by gravimetric method to maintain 60% of field capacity.
[0086] Treatment 2 (Non-sterilized Soil): Accurately weigh 300g of treated soil sample and place it in a 500mL Erlenmeyer flask, sealed with breathable film. Under sterile conditions, add an inorganic salt liquid medium containing penthiopyrad (final concentration of penthiopyrad is 50mg / kg) to bring the soil moisture content to 60% of field capacity, and mix the soil thoroughly. A suspension of degrading bacteria was added to the experimental group and mixed thoroughly with the soil. A separate soil sample without degrading bacteria at the same concentration served as a blank control. Sampling time points and testing methods were the same as for Treatment 1. Throughout the experiment, water was continuously replenished by weighing to maintain 60% of field capacity.
[0087] 2. The test results are as follows: Figure 9 and Figure 10 The degradation effects of the degrading bacteria on penthiopyrad varied little, demonstrating their ability to effectively promote the degradation of penthiopyrad. The degradation process of strain PEB1 exhibited a two-stage pattern: a lag phase of 0-3 days followed by rapid degradation. After 60 days of incubation, the degradation rate in sterilized soil inoculated with the degrading bacteria PEB1 was 52.3%, significantly higher than that of the sterilized control (46.5%, P < 0.05). Under non-sterile conditions, the degradation efficiency of the degrading bacteria PEB1 (60.4%) was 8.7% higher than that of the non-sterile control (51.7%) (P < 0.05), and the residual concentration in soil was significantly reduced.
[0088] The results showed that the degradation performance of strain PEB1 was stable after being directly applied to the soil, providing a scientific basis for the soil remediation of penthiopyrad by strain PEB1.
[0089] The above embodiments describe the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for illustrating the principles of the present invention. Without departing from the scope of the principles of the present invention, the present invention may have various changes and improvements, and these changes and improvements all fall within the scope of protection of the present invention.
Claims
1. A strain of Stenotrophomonas maltophilia that can degrade penthiopyrad Stenotrophomonas maltophilia ) strain PEB1, characterized in that: The strain PEB1 was deposited in the General Microbiology Center of China Culture Collection Administration on December 24, 2024, with the deposit number: CGMCC No.33180.
2. Use of the Stenotrophomonas maltophilia strain PEB1 according to claim 1 in degrading penthiopyrad.
3. Use of the Stenotrophomonas maltophilia strain PEB1 according to claim 1 in repairing a natural environment contaminated by the fungicide penthiopyrad or in preparing a penthiopyrad-degrading bacterial agent.
4. The application according to claim 3, characterized in that The natural environment is water or soil.
5. A bacterial agent for efficiently degrading penthiopyrad, characterized in that: Contains the Stenotrophomonas maltophilia strain PEB1 according to claim 1.