Composition and anti-plague and disease-resistant probiotic fermented feed

Abstract

The invention discloses a composition and an anti-plague and disease-resistant probiotic fermented feed. The composition comprises the following components in percentage by weight: 25%-35% of pinecone bark, 15%-25% of peanut shell powder, 3%-7% of soybean meal, 3%-7% of chicken blood powder, 7%-13% of bean dregs, 7%-13% of soy sauce residues, 0.5%-0.7% of illite, 7%-13% of bacillus subtilis, 0.0003%-0.0007% of double-oxin oligopeptide fertilizer and the balance of water. Wherein the pine cone bark is fermented pine cone bark. The anti-pestilence and disease-resistant probiotic fermented feed prepared from the composition can prevent poison and epidemic diseases, improve immunity and effectively resist swine fever.

Description

Technical Field

[0001] The present application relates to the field of biological breeding technology, and in particular to a composition and a plague-proof and disease-resistant probiotic fermented feed. Background Art

[0002] Probiotic feed technology is a crucial area at the intersection of agricultural biotechnology and animal nutrition. Traditional fermented feed has a centuries-old folk tradition, paved with breakthroughs in microbiology in the early 20th century. By 1980, industrialized bacterial culture technology had matured, evolving from single strains to complex strains, and from solid-state fermentation to submerged fermentation. Probiotic feed technology offers a solution to addressing global food security, environmental pollution, and healthy animal husbandry.

[0003] Existing technologies use probiotics (such as lactic acid bacteria, yeast, and Bacillus) to ferment feed ingredients anaerobically or aerobically, breaking down macromolecules (cellulose, protein, etc.) into small molecule nutrients (organic acids, enzymes, vitamins, etc.), improving feed palatability, digestibility, and nutritional value while inhibiting the growth of pathogens. Although probiotic fermented feed has significant advantages in improving feed utilization and animal health, it still has many drawbacks and shortcomings in practical applications, mainly in the following aspects:

[0004] 1. Insufficient bacterial activity and stability

[0005] High temperature inactivation: High temperatures (above 60°C) during feed processing (such as pelleting and puffing) can easily lead to the death of a large number of probiotics and a significant decrease in the number of live bacteria.

[0006] Storage attenuation: The number of viable bacteria may drop by more than 50% during long-term storage (especially in summer), affecting the terminal effect.

[0007] 2. Fermentation process control is complex

[0008] Parameter sensitivity: Slight deviations in fermentation temperature, humidity, and sealing can easily lead to bacterial contamination (such as mold and spoilage bacteria), causing feed deterioration.

[0009] Raw material limitations: The fermentation efficiency of high-fiber (such as straw) or high-fat raw materials is low, and additional enzyme preparations or pretreatment equipment are required, which increases costs.

[0010] 3. Difficulty in standardization

[0011] The fermentation endpoint is unclear: the fermentation time varies greatly with different raw materials and seasons, and there is a lack of unified physical and chemical indicators (such as pH value and organic acid content) for judgment.

[0012] 4. Differences in palatability

[0013] Too high acidity: Excessive fermentation leads to a low pH (<4.0), and some animals (such as young animals) may refuse to eat or have diarrhea.

[0014] 5. Nutritional imbalance: Completely replacing conventional feed can easily lead to an imbalance in energy or protein ratios, and the amount added needs to be controlled (usually ≤30%), resulting in limited room for cost reduction.

[0015] Therefore, there is an urgent need in this field to develop new functional probiotic fermented feeds in order to effectively enhance animal immunity, increase nutrition, and have antibacterial and anti-inflammatory effects, and to solve and optimize the deficiencies of probiotic feeds. Summary of the Invention

[0016] Based on this, at least one composition and anti-emergency and disease-resistant probiotic fermented feed are provided.

[0017] In one aspect of the present application, a composition for preparing a blast-resistant and disease-resistant probiotic fermented feed is provided, comprising the following components in percentage by weight:

[0018] Pine cone bark 25%-35%, peanut shell powder 15%-25%, soybean meal 3%-7%, chicken blood powder 3%-7%, soybean dregs 7%-13%, soybean residue 7%-13%, soybean paste residue 7%-13%, illite 0.5%-0.7%, Bacillus subtilis 7%-13%, Shuangxin oligofertilizer 0.0003%-0.0007%, and water;

[0019] Wherein, the pine cone bark is fermented pine cone bark.

[0020] In some embodiments, the composition comprises the following components in weight percentage: 28% to 32% of pine cone bark, 18% to 22% of peanut shell powder, 4% to 6% of soybean meal, 4% to 6% of chicken blood powder, 8% to 12% of bean dregs, 8% to 12% of soybean residue, 0.55% to 0.65% of illite, 8% to 12% of Bacillus subtilis, 0.0004% to 0.0006% of Shuangxin oligofertilizer, and water.

[0021] In some embodiments, the composition comprises the following components in weight percentages:

[0022] Pine cone bark 30%, peanut shell powder 20%, soybean meal 5%, chicken blood powder 5%, bean dregs 10%, soybean residue 10%, soybean paste residue 10%, Bacillus subtilis 10%, illite 0.6%, Shuangxin oligofertilizer 0.0005%, and water.

[0023] In some embodiments, the fermentation of pine cone bark comprises:

[0024] Mix unfermented pine cone bark with soil and organic matter, and control the fermentation conditions to 50% to 60% humidity, 25°C to 30°C, and ferment for 3 to 6 months;

[0025] The relative amounts of the unfermented pine cone bark, the soil and the organic matter are (6-7):(1-2):(3-4); and the organic matter includes one or more of bean cake, sawdust and dead leaves.

[0026] Another aspect of the present application provides a method for preparing a blast-resistant and disease-resistant probiotic fermented feed, comprising uniformly mixing raw materials comprising the following components in the following weight percentages and then subjecting them to biological fermentation to obtain the blast-resistant and disease-resistant probiotic fermented feed;

[0027] The raw materials include: 25% to 35% of pine cone bark, 15% to 25% of peanut shell powder, 3% to 7% of soybean meal, 3% to 7% of chicken blood powder, 7% to 13% of bean dregs, 7% to 13% of soybean residue, 0.5% to 0.7% of illite, 7% to 13% of Bacillus subtilis, 0.0003% to 0.0007% of Shuangxin oligofertilizer, and water;

[0028] Wherein, the pine cone bark is fermented pine cone bark.

[0029] In some embodiments, the raw materials include: 28%~32% of pine cone bark, 18%~22% of peanut shell powder, 4%~6% of soybean meal, 4%~6% of chicken blood powder, 8%~12% of bean dregs, 8%~12% of soybean residue, 0.55%~0.65% of illite, 8%~12% of Bacillus subtilis, 0.0004%~0.0006% of Shuangxin oligofertilizer, and water.

[0030] In some embodiments, the raw materials include: 30% pine cone bark, 20% peanut shell powder, 5% soybean meal, 5% chicken blood powder, 10% bean dregs, 10% soybean residue, 10% soybean dregs, 10% soybean paste residue, 10% Bacillus subtilis, 0.6% illite, 0.0005% Shuangxin oligofertilizer, and water.

[0031] In some embodiments, the conditions for biological fermentation include: temperature 30° C. to 35° C., humidity 55% to 60%, and pH 6.5 to 7.0.

[0032] In some embodiments, the fermentation of pine cone bark comprises:

[0033] Mix unfermented pine cone bark with soil and organic matter, and control the fermentation conditions to 50% to 60% humidity, 25°C to 30°C, and ferment for 3 to 6 months;

[0034] The relative amounts of the unfermented pine cone bark, the soil and the organic matter are (6-7):(1-2):(3-4); and the organic matter includes one or more of bean cake, sawdust and dead leaves.

[0035] Another aspect of the present application provides a plague-proof and disease-resistant probiotic fermented feed prepared using the preparation method described above.

[0036] Another aspect of the present application provides a method for raising non-human animals, which comprises administering the aforementioned anti-blast and disease-resistant probiotic fermented feed to the non-human animals.

[0037] The anti-plague and disease-resistant probiotic fermented feed provided in one aspect of the present application has at least the following advantages:

[0038] The inventor creatively combined feed raw materials such as pine cone bark, peanut shell powder and soybean meal with Bacillus subtilis, illite and Shuangxin oligofertilizer. The components worked synergistically to finally prepare a plague-proof and disease-resistant probiotic fermented feed that can prevent toxins and epidemics, enhance immunity and effectively resist swine fever.

[0039] Specifically:

[0040] 1) Disease Control: Reduces the risk of disease transmission and offers epidemic prevention and disease resistance. For high-fatality livestock epidemics (such as African swine fever and avian influenza), Bacillus subtilis can inhibit the colonization of pathogens (viruses and bacteria) in the intestines, reducing viral loads and indirectly lowering the risk of infection in animal populations. Shuangxin Oligo Fertilizer supplements animal nutrition and boosts immunity. Illite's physical anti-inflammatory properties enhance epidemic prevention and disease resistance.

[0041] 2) Immune regulation: Probiotic metabolites (such as short-chain fatty acids and antimicrobial peptides) can activate the animal immune system (such as enhancing IgA secretion and increasing macrophage activity), thereby improving resistance to plague and other infectious diseases.

[0042] 3) Antibiotic Alternatives: Promoting green and healthy farming and reducing reliance on antibiotics. Probiotics can replace antibiotics in preventing disease and alleviating drug resistance by competitively excluding pathogens and regulating the balance of intestinal flora.

[0043] 4) Improve production performance: The economic benefits are significant and the feed utilization rate is improved. During the fermentation process, probiotics decompose anti-nutritional factors (such as phytic acid and cellulose) in the feed, releasing small molecule nutrients (amino acids and monosaccharides), and improving digestion and absorption rate.

[0044] 5) Growth-promoting effect: Vitamins (B group, K), enzymes, etc. produced by probiotic metabolism can promote animal growth and shorten the marketing cycle (can increase the daily weight gain of pigs by 5%-10%).

[0045] 6) Reduce mortality: Reduce mortality due to plague or secondary infection through intestinal health management and disease prevention.

[0046] 7) Improve animal welfare and product quality. Optimize intestinal health: Probiotics maintain the balance of intestinal flora, reduce digestive system diseases such as diarrhea and flatulence, and improve the physiological state of animals.

[0047] 8) Improved meat safety: Reduce antibiotic residues and pathogenic microorganism contamination, meeting consumers' demand for safe livestock products.

[0048] 9) Environmental protection and sustainable development: Reduce manure pollution. Probiotics improve feed utilization, reduce nitrogen and phosphorus emissions in manure (reduce the generation of harmful gases such as ammonia and hydrogen sulfide), and alleviate environmental pollution.

[0049] 10) Resource recycling: Agricultural by-products (such as straw and soybean meal) can be used for fermentation to reduce feed costs and promote a circular economy.

[0050] 11) Technical implementation path: Strain screening, the rational combination of feed ingredients and strains can produce a synergistic effect. At the same time, the addition of Shuangxin oligofertilizer biological water-soluble technology and illite physical anti-inflammatory is the technical core of this anti-epidemic and disease-resistant probiotic fermented feed.

[0051] 12) Fermentation process optimization: Use solid-state or liquid fermentation technology to control parameters such as temperature, humidity, and pH to maximize the release of functional metabolites.

[0052] 13) Functional Verification: Animal experiments are conducted to verify the effectiveness of the anti-plague and disease-resistant probiotic fermented feed in preventing and controlling specific plague diseases.

[0053] 14) Classical Swine Fever Prevention and Control Trial: Adding this anti-swine fever and disease-resistant probiotic to fermented feed can reduce the prevalence of Classical Swine Fever virus in pig herds. DETAILED DESCRIPTION

[0054] For ease of understanding of the present application, the present application will be described more fully below with reference to the embodiments. However, the present application can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present application more thorough and comprehensive.

[0055] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this application pertains. The terms used herein in the specification of this application are for the purpose of describing specific embodiments only and are not intended to limit this application. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0056] In this application, unless otherwise specified, "one or more" refers to any one of the listed items or any combination of the listed items. Similarly, "one or more" and other similar expressions that refer to "one or more" are also understood in the same way unless otherwise specified.

[0057] The terms "combination thereof", "any combination thereof", "any combination thereof" and the like used in this application include all suitable combinations of any two or more of the listed items.

[0058] In this application, the word "suitable" in "suitable combination", "suitable method", "any suitable method", etc. shall be based on the ability to implement the technical solution of this application, solve the technical problems of this application, and achieve the expected technical effects of this application.

[0059] In this application, the terms "further," "further," "particularly," "for example," "such as," "example," and "for example" are used for descriptive purposes to indicate that the preceding and following technical solutions are related in terms of the content covered, but should not be construed as limiting the preceding technical solution or the scope of protection of this application. In this application, unless otherwise specified, A (such as B) means that B is a non-limiting example of A, and it is understood that A is not limited to B.

[0060] In this application, the terms "optionally," "optional," and "optional" mean optional, that is, they refer to either option selected from the two parallel options of "yes" or "no." If multiple "options" appear in a technical solution, unless otherwise specified and there are no contradictions or mutual constraints, each "optional" is independent. Unless otherwise specified, the descriptions "optionally include," "optionally include," etc. in this application, taking "optionally include" as an example, mean "may include or not include."

[0061] As used herein, the terms "comprising," "including," and "include" are synonymous and are inclusive or open-ended, not excluding additional, unrecited members or features. Examples of members or features include materials or components, structures, elements, and instruments. Non-limiting examples of members or features include actions, conditions for the occurrence of actions, timing, and states.

[0062] In this application, the technical features or technical solutions described in open language include closed technical features or technical solutions composed of the listed contents, and also include open technical features or technical solutions containing the listed contents.

[0063] In this application, exemplary descriptions such as "in some embodiments (or examples)" and "in one embodiment (or example)" may include but are not limited to the following meanings: these solutions can be combined with other solutions in a suitable manner to form new technical solutions.

[0064] In this application, the terms "first," "second," "third," "fourth," etc. in "the first aspect," "the second aspect," "the third aspect," "the fourth aspect," etc. are used for descriptive purposes only and should not be understood as indicating or implying relative importance or quantity, nor should they be understood as implicitly indicating the importance or quantity of the indicated technical features. Moreover, "first," "second," "third," "fourth," etc. serve only for the purpose of non-exhaustive enumeration and description, and should be understood not to constitute a closed-ended limitation on quantity.

[0065] In this application, when referring to a numerical interval (i.e., a numerical range), unless otherwise specified, the distribution of the optional numerical values ​​in the numerical interval is considered to be continuous and includes the two numerical endpoints (i.e., the minimum and maximum values) of the numerical interval, and each numerical value between the two numerical endpoints. Unless otherwise specified, when a numerical interval only refers to an integer in the numerical interval, including the two endpoint integers of the numerical range and each integer between the two endpoints, is equivalent to directly enumerating each integer. When multiple numerical ranges are provided to describe a feature or characteristic, these numerical ranges can be merged. In other words, unless otherwise specified, the numerical range disclosed herein should be understood to include any and all subranges included therein. The "numerical value" in the numerical interval can be any quantitative value, such as a number, a percentage, a ratio, etc. "Numerical interval" allows broadly including numerical interval types such as percentage intervals, ratio intervals, and ratio intervals.

[0066] In this application, if a method flow involves multiple steps, unless otherwise specified herein, there is no strict order restriction on the execution of these steps, and they can be executed in an order other than the order described. Moreover, any step can include multiple sub-steps or multiple stages, and these sub-steps or stages do not necessarily need to be completed at the same time, but can be executed at different times, and their execution order is not necessarily sequential, but can be executed in turn, alternating, or simultaneously with other steps or parts of sub-steps or stages of other steps.

[0067] One aspect of the present application provides a plague-proof and disease-resistant probiotic fermented feed, the core technology of which includes: a reasonable combination of feed ingredients and bacterial strains to produce a synergistic effect, while adding Shuangxin oligofertilizer biological water-soluble technology and illite physical antibacterial.

[0068] The innovative nature of this anti-plague and disease-resistant probiotic fermented feed also lies in the optimization of feed processing and production techniques, the screening of bacterial strains, the control of the fermentation process, and the in-depth research and development of probiotic fermented feed with anti-plague and disease-resistant properties. The use of advanced solid-state fermentation technology can significantly improve production efficiency and ensure the stability of product quality. In terms of bacterial strain screening, the latest probiotic strains and genetic engineering technologies are used to improve bacterial strain characteristics to achieve better fermentation performance. Furthermore, the innovative addition of Shuangxin oligofertilizer biological water-soluble technology and illite physical antibacterial agents allows the production of fermented feed products that better meet animal needs. Furthermore, real-time monitoring and intelligent regulation technologies can be used in fermentation process control to precisely control fermentation conditions and optimize metabolite composition, making this a unique innovation.

[0069] The practicality of the aforementioned anti-bacteria and disease-resistant probiotic fermented feed is at least reflected in its unique nutritional value and functional properties. Through the fermentation action of probiotics, macromolecular nutrients in the feed are broken down into smaller molecules that are more easily digested and absorbed, while also producing a variety of beneficial metabolites, such as vitamins, organic acids, and antimicrobial peptides. These changes not only increase the nutritional value of the feed but also enhance its health benefits. Studies have shown that probiotic fermented feed can improve the balance of the animal's intestinal microecology, enhance immune function, and improve disease resistance. It can also promote animal growth, increase feed conversion rate, and improve product quality. These characteristics give anti-bacteria and disease-resistant probiotic fermented feed significant advantages in improving animal health and production performance.

[0070] The wide applicability of this probiotic fermented feed for plague prevention and disease resistance stems from its disease resistance and adaptability across various animal species and farming models. By optimizing production processes, selecting superior strains, and improving fermentation technology, this probiotic fermented feed possesses both plague prevention and disease resistance. In livestock and poultry farming, it can be widely used in the feeding of pigs, chickens, cattle, sheep, and other animals.

[0071] Pine cone bark (the outer scales or shells of pine cones) is rich in cellulose and lignin, as well as minerals and trace elements such as iron, phosphorus, and calcium. These ingredients help promote intestinal motility, improve digestion, and assist in regulating cholesterol levels. Fermentation converts pine cone bark into humus, releasing nutrients, indirectly indicating its rich organic matter content. Pine cone bark also contains antioxidants such as vitamin E and polyphenols, which help scavenge free radicals, slow cell aging, and repair cells. Pine cone bark also contains medicinal ingredients, including volatile oils and pinitol, which have antibacterial and anti-inflammatory properties, relieving respiratory inflammation and coughs. Flavonoids have antioxidant and anti-tumor potential, benefiting cardiovascular health. Resin and rosin are used to dispel wind and dampness, and topical application can reduce joint swelling and pain. Pine cone bark is unique in its market application as an ingredient in fermented probiotic feed for plague prevention and disease resistance.

[0072] Peanut shell meal, a byproduct of peanut processing, has nutritional value as a feed ingredient. Due to its high fiber content, it serves as a roughage or fiber supplement. Its nutritional value and application characteristics include: Crude fiber (30-50%): Peanut shell meal's primary components are cellulose, hemicellulose, and lignin, which promote intestinal health. Crude protein (5-8%): It can be used in combination with other high-protein feeds (such as soybean meal and fish meal). Minerals: Contains calcium, phosphorus, potassium, and magnesium. Fat (1-2%): Other ingredients: Contains small amounts of polyphenols (such as tannins). It is suitable for use in energy feeds (corn, wheat), protein feeds (soybean meal), and mineral premixes. Microbial fermentation can degrade lignin, improving fiber utilization.

[0073] Soybean meal, a byproduct of soybean oil extraction (primarily through extraction or pressing), is a core plant-based protein ingredient in the feed industry. Due to its high protein content, balanced amino acid profile, and economical efficiency, it is widely used in livestock, poultry, and aquaculture feeds. The following is a detailed analysis of its nutritional value and applications: Soybean meal protein has a digestibility of 85-90% (higher than other plant proteins), making it a key alternative to fishmeal. It also boasts a high lysine content (approximately 2.8%) and a fiber content of only 3-7%, making it suitable for the digestive systems of monogastric animals (pigs and poultry). Its cost-effectiveness significantly surpasses that of fishmeal, making it a core protein ingredient for large-scale aquaculture. Fermentation with lactic acid bacteria or Bacillus subtilis degrades antigenic proteins and increases the content of small peptides.

[0074] Chicken blood meal is an animal protein feed ingredient made from coagulated and dried chicken blood. It is high in protein and rich in iron. Its nutritional value lies in its high protein and iron content, making it suitable for anemic or rapidly growing animals (such as piglets and young poultry). Its functional ingredient, hemoglobin, improves intestinal oxygen supply and promotes digestion and absorption. Resource utilization reduces slaughterhouse waste pollution and lowers feed costs.

[0075] Okara (tofu dregs) is the residue from soybean production, such as soy milk, tofu, and soy milk. Its primary components are the soybean husks and unextracted fiber and protein. It's cheaper than soybean meal and suitable as a partial replacement for plant protein. It promotes gastrointestinal motility and improves intestinal health in animals.

[0076] Soy sauce residue is the residue left after the production of fermented condiments like soy sauce and fermented broad bean paste. It contains a high amount of salt and fermentation products. It also contains probiotics and organic acids, which improve the intestinal microbiome and enhance palatability. The flavoring substances (nucleotides and glutamate) produced by fermentation promote food intake.

[0077] Shuangxin Oligo Fertilizer is rich in trace elements such as zinc, iron, manganese, calcium, and magnesium. Its water-soluble technology rapidly activates the animal's immune system (inducing disease resistance), combining nutrients with biostimulants to enhance resistance. The combination of oligosaccharides and a biological agent (Bacillus subtilis) in Shuangxin Oligo Fertilizer is the technological highlight of Songsu No. 1, a disease-resistant probiotic fermented feed.

[0078] Illite is a yellowish-white powdered mica composed of over 20 minerals, including silicon, strontium, aluminum, and calcium. Originating in China, illite produced there contains far more minerals than those found in EU countries. Extensive research and experiments have demonstrated that illite is not only effective in absorbing, removing, and decomposing heavy metals and toxic gases, but also has antiviral and immune-boosting properties. It also releases far-infrared radiation and negative ions, which are beneficial to humans, animals, and plants. Illite possesses antibacterial properties due to its unique structure and is rich in numerous rare and beneficial trace elements, such as strontium and potassium. The strontium content reaches 90mg / kg, a level unmatched by any other mineral. On January 4, 2019, the European Commission issued Regulation (EU) 2019 / 8-2019 / 12, approving substances such as natural mixed preparations of illite-montmorillonite-kaolinite as animal feed additives in accordance with Regulation (EC) No 1831 / 2003 of the European Parliament and of the Council. The physical antibacterial properties of illite further improve the nutritional and disease-resistant functional properties of fermented feed.

[0079] In one aspect of the present application, a composition for preparing a plague-resistant and disease-resistant probiotic fermented feed is provided, which comprises pine cone bark, peanut shell powder, soybean meal, chicken blood powder, bean dregs, sauce residue, illite, Bacillus subtilis, Shuangxin oligofertilizer, and water.

[0080] In some embodiments, the composition comprises the following components in weight percentages:

[0081] Pine cone bark 25%-35%, peanut shell powder 15%-25%, soybean meal 3%-7%, chicken blood powder 3%-7%, soybean dregs 7%-13%, soybean residue 7%-13%, soybean paste residue 7%-13%, illite 0.5%-0.7%, Bacillus subtilis 7%-13%, Shuangxin oligofertilizer 0.0003%-0.0007%, and water;

[0082] Wherein, the pine cone bark is fermented pine cone bark.

[0083] Illustratively, the weight ratio of pine cone bark in the composition is 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, or a range or value between any two values.

[0084] Illustratively, the weight ratio of peanut shell powder in the composition is 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, or a range or value between any two values.

[0085] Illustratively, the weight ratio of soybean meal in the composition is 3%, 4%, 5%, 6%, 7%, or a range or value between any two values.

[0086] Illustratively, the weight ratio of chicken blood powder in the composition is 3%, 4%, 5%, 6%, 7%, or a range or value between any two values.

[0087] Illustratively, the weight ratio of okara in the composition is 7%, 8%, 9%, 10%, 11%, 12%, 13%, or a range or value between any two values.

[0088] Illustratively, the weight ratio of the sauce residue in the composition is 7%, 8%, 9%, 10%, 11%, 12%, 13%, or a range or value between any two values.

[0089] Illustratively, the weight ratio of illite in the composition is 0.5%, 0.6%, 0.7%, or a range or value between any two values.

[0090] Illustratively, the weight ratio of Bacillus subtilis in the composition is 7%, 8%, 9%, 10%, 11%, 12%, 13%, or a range or value between any two values.

[0091] Illustratively, the weight ratio of Shuangxin Oligo Fertilizer in the composition is 0.0003%, 0.0004%, 0.0005%, 0.0006%, 0.0007%, or a range or value between any two values.

[0092] In some embodiments, the composition comprises the following components in weight percentage: 28% to 32% of pine cone bark, 18% to 22% of peanut shell powder, 4% to 6% of soybean meal, 4% to 6% of chicken blood powder, 8% to 12% of bean dregs, 8% to 12% of soybean residue, 0.55% to 0.65% of illite, 8% to 12% of Bacillus subtilis, 0.0004% to 0.0006% of Shuangxin oligofertilizer, and water.

[0093] In some embodiments, the composition comprises the following components in weight percentages:

[0094] Pine cone bark 30%, peanut shell powder 20%, soybean meal 5%, chicken blood powder 5%, bean dregs 10%, soybean residue 10%, soybean paste residue 10%, Bacillus subtilis 10%, illite 0.6%, Shuangxin oligofertilizer 0.0005%, and water.

[0095] In some embodiments, the fermentation of pine cone bark comprises:

[0096] Mix unfermented pine cone bark with soil and organic matter, and control the fermentation conditions to 50% to 60% humidity, 25°C to 30°C, and ferment for 3 to 6 months;

[0097] The relative amounts of the unfermented pine cone bark, the soil and the organic matter are (6-7):(1-2):(3-4); and the organic matter includes one or more of bean cake, sawdust and dead leaves.

[0098] Another aspect of the present application provides a method for preparing a blast-resistant and disease-resistant probiotic fermented feed, comprising mixing raw materials comprising the following components in the following weight percentages and then subjecting them to biological fermentation to obtain the blast-resistant and disease-resistant probiotic fermented feed;

[0099] The raw materials include: 25% to 35% of pine cone bark, 15% to 25% of peanut shell powder, 3% to 7% of soybean meal, 3% to 7% of chicken blood powder, 7% to 13% of bean dregs, 7% to 13% of soybean residue, 0.5% to 0.7% of illite, 7% to 13% of Bacillus subtilis, 0.0003% to 0.0007% of Shuangxin oligofertilizer, and water;

[0100] Wherein, the pine cone bark is fermented pine cone bark.

[0101] The content of each component in the raw materials can be referred to the above definitions.

[0102] In some embodiments, the raw materials include: 28%~32% of pine cone bark, 18%~22% of peanut shell powder, 4%~6% of soybean meal, 4%~6% of chicken blood powder, 8%~12% of bean dregs, 8%~12% of soybean residue, 0.55%~0.65% of illite, 8%~12% of Bacillus subtilis, 0.0004%~0.0006% of Shuangxin oligofertilizer, and water.

[0103] In some embodiments, the raw materials include: 30% pine cone bark, 20% peanut shell powder, 5% soybean meal, 5% chicken blood powder, 10% bean dregs, 10% soybean residue, 10% soybean dregs, 10% soybean paste residue, 10% Bacillus subtilis, 0.6% illite, 0.0005% Shuangxin oligofertilizer, and water.

[0104] In some embodiments, the conditions for biological fermentation include: a temperature of 30°C to 35°C, a humidity of 55% to 60%, and a pH of 6.5 to 7.0. For example, the temperature may be 30°C, 31°C, 32°C, 33°C, 34°C, 35°C, or a range or value between any two values. The humidity may be, for example, 55%, 56%, 57%, 58%, 59%, 60%, or a range or value between any two values. The pH may be, for example, 6.5, 6.6, 6.7, 6.8, 6.9, 7.0, or a range or value between any two values.

[0105] In some embodiments, the fermentation of pine cone bark comprises:

[0106] Mix unfermented pine cone bark with soil and organic matter, and control the fermentation conditions to 50% to 60% humidity, 25°C to 30°C, and ferment for 3 to 6 months;

[0107] The relative amounts of the unfermented pine cone bark, the soil and the organic matter are (6-7):(1-2):(3-4); and the organic matter includes one or more of bean cake, sawdust and dead leaves.

[0108] In some embodiments, the relative amounts of the unfermented pine cone bark, the soil, and the organic matter are 6.5:1.5:3.5.

[0109] During the fermentation of pine cone bark, the humidity can be, for example, 50%, 51%, 52%, 53%, 54%, 55%, or any range or value between any two values. The temperature can be, for example, 25° C., 26° C., 27° C., 28° C., 29° C., 30° C., or any range or value between any two values.

[0110] In another aspect of the present application, provided is a blast-proof and disease-resistant probiotic fermented feed prepared using the preparation method described above.

[0111] In another aspect of the present application, a method for raising non-human animals is provided, wherein the non-human animals are given the aforementioned anti-blast and disease-resistant probiotic fermented feed.

[0112] The non-human animals may be, for example, common livestock, such as pigs, horses, cows, and sheep.

[0113] Some examples are provided below.

[0114] The embodiments of the present application will be described in detail below with reference to the examples. It should be understood that these examples are intended to illustrate the present application only and are not intended to limit the scope of the present application. The experimental methods for which the conditions are not specified in the following examples are preferably referred to the guidance provided in the present application, and may also be based on the experimental manuals or conventional conditions in this area, or on the conditions recommended by the manufacturer, or with reference to experimental methods known in the art.

[0115] Unless otherwise specified, “%” refers to mass percentage.

[0116] The peanut shell powder, soybean meal, chicken blood powder, bean dregs, illite, Shuangxin oligofertilizer and soy sauce residue used below are all purchased from outside the market.

[0117] The Shuangxin oligofertilizer used below was from the Chinese Academy of Sciences.

[0118] The manufacturer of the Bacillus subtilis used below is Weifang Yihao Biotechnology Co., Ltd., with license number Lu Shitian (2021) H07344.

[0119] The common feeds used below were purchased from Weifang Yihao Biotechnology Co., Ltd. with the license number Lu Shitian (2021) H07344.

[0120] The following anti-plague and disease-resistant probiotic fermented feed has been tested by Henan Taiqing Quality Inspection Co., Ltd. and issued a quality inspection certificate. All tests have met national requirements and standards.

[0121] Example 1 Preparation and application of anti-plague and disease-resistant probiotic fermented feed

[0122] The composition ratio of anti-plague and disease-resistant probiotic fermented feed:

[0123] Pine cone bark 30%, peanut shell powder 20%, soybean meal 5%, chicken blood powder 5%, bean dregs 10%, soybean residue 10%, soybean sauce residue 10%, illite 0.6%, Shuangxin oligofertilizer 0.0005%, Bacillus subtilis 10%, and drinking water 9.3995%.

[0124] The preparation of pine cone bark comprises:

[0125] Mix unfermented pine cone bark with soil and organic matter, and control the fermentation conditions to 55% humidity, 28°C, and ferment for 4 months;

[0126] Among them, the relative amounts of unfermented pine cone bark, soil and organic matter are 6.5:1.5:3.5; the organic matter is bean cake.

[0127] Through biological fermentation, biological fermentation material (hereinafter referred to as Songsu No. 1) is formed.

[0128] Biofermentation is a solid-state fermentation method, using Bacillus subtilis to ferment the feed. During the fermentation process, the temperature is controlled at 30°C to 35°C. This temperature range is conducive to the rapid reproduction and metabolism of Bacillus subtilis, exerting its effects in regulating intestinal flora and enhancing immunity. Humidity is maintained at 55% to 60%, ensuring the appropriate dryness and humidity of the material, which not only meets the growth requirements of microorganisms but also prevents excessive moisture from breeding bacteria. It provides sufficient moisture for microorganisms while ensuring the stability of the material structure. The pH value is stable at 6.5 to 7.0, which is within the optimal growth range for Bacillus subtilis. This ensures efficient and stable fermentation throughout the entire process. The resulting feed is nutritious and palatable, promoting the healthy growth of livestock and poultry and improving breeding efficiency.

[0129] Test case:

[0130] Experimental site: Yang Baogang Pig Farm, No. 8, Group 2, Honglian Village, Mingshan District, Benxi City, Liaoning Province;

[0131] Test period: December 22, 2024 - January 5, 2024 (15 days);

[0132] Experimental species: Duroc fattening pigs;

[0133] Total number of pigs on the farm: 43 (weighing 200-220 catties);

[0134] Operational procedures: One pig on the farm was infected with swine fever. After isolation and disinfection, the remaining 42 pigs were placed in the same pen. Forty pigs were fed standard feed, while two pigs were marked and fed Songsu No. 1 anti-swine fever probiotic fermented feed. See Table 1 for details.

[0135] Table 1

[0136]

[0137] Analysis of test results:

[0138] I. Environmental Analysis: Classical swine fever is a highly contagious viral disease. Once infected, the virus survives and spreads rapidly within the same piggery environment, and there is an incubation period. In this experiment, 42 pigs were housed in the same pen and fed simultaneously. The pigs' living environment was consistent, and there were no environmental factors affecting their survival.

[0139] Second, time analysis: The swine fever virus can survive for months or even years in a cool, dry environment. This experiment lasted 15 days, which is short, and the virus won't automatically decompose in such a short time. In the same pen, 40 of the 42 pigs died, while only two survived. Time is not a factor.

[0140] 3. Disinfection method: The pens are uniformly disinfected with potassium persulfate composite powder and cleaned regularly. There is no factor affecting the disinfection method.

[0141] 4. Analysis and summary: The same environment, the same time, and uniform disinfection, only the feeding method is different, and the results are different. 40 pigs were fed with the original feeding method, and all 40 test pigs died. 2 pigs were fed Songsu No. 1 anti-plague and disease-resistant probiotic fermented feed, and 2 survived and were not infected with the plague after testing. The experiment shows that Songsu No. 1 anti-plague and disease-resistant probiotic fermented feed has the effects of preventing viruses and epidemics, enhancing immunity, and effectively resisting swine fever. The various technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the various technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0142] The above-described embodiments merely represent several implementation methods of the present application. While the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent. It should be noted that a person skilled in the art could make various modifications and improvements without departing from the spirit of the present application, all of which fall within the scope of protection of the present application. Therefore, the scope of protection of the present patent application shall be determined by the appended claims, and the specification shall be used to interpret the content of the claims.

Claims

1. A composition for preparing a pest-resistant and disease-resistant probiotic fermented feed, characterized in that: It contains the following components in weight percentage: Pine cone bark 25%-35%, peanut shell powder 15%-25%, soybean meal 3%-7%, chicken blood powder 3%-7%, soybean dregs 7%-13%, soybean residue 7%-13%, soybean paste residue 7%-13%, illite 0.5%-0.7%, Bacillus subtilis 7%-13%, Shuangxin oligofertilizer 0.0003%-0.0007%, and water; Wherein, the pine cone bark is fermented pine cone bark.

2. The composition according to claim 1, wherein The invention comprises the following components in percentage by weight: 28% to 32% of pine cone bark, 18% to 22% of peanut shell powder, 4% to 6% of soybean meal, 4% to 6% of chicken blood powder, 8% to 12% of bean dregs, 8% to 12% of soybean residue, 0.55% to 0.65% of illite, 8% to 12% of Bacillus subtilis, 0.0004% to 0.0006% of Shuangxin oligofertilizer, and water. Optionally, it comprises the following components in weight percentage: Pine cone bark 30%, peanut shell powder 20%, soybean meal 5%, chicken blood powder 5%, bean dregs 10%, soybean residue 10%, soybean paste residue 10%, Bacillus subtilis 10%, illite 0.6%, Shuangxin oligofertilizer 0.0005%, and water.

3. The composition according to claim 1 or 2, wherein The fermentation of the pine cone bark comprises: Mix unfermented pine cone bark with soil and organic matter, and control the fermentation conditions to 50% to 60% humidity, 25°C to 30°C, and ferment for 3 to 6 months; The relative amounts of the unfermented pine cone bark, the soil and the organic matter are (6-7):(1-2):(3-4); and the organic matter includes one or more of bean cake, sawdust and dead leaves.

4. A method for preparing a plague-resistant and disease-resistant probiotic fermented feed, characterized in that: The method comprises the steps of: uniformly mixing raw materials containing the following components in the following weight percentages and then performing biological fermentation to obtain anti-bacteria and anti-disease probiotic fermented feed; The raw materials include: 25% to 35% of pine cone bark, 15% to 25% of peanut shell powder, 3% to 7% of soybean meal, 3% to 7% of chicken blood powder, 7% to 13% of bean dregs, 7% to 13% of soybean residue, 0.5% to 0.7% of illite, 7% to 13% of Bacillus subtilis, 0.0003% to 0.0007% of Shuangxin oligofertilizer, and water; Wherein, the pine cone bark is fermented pine cone bark.

5. The preparation method according to claim 4, wherein The raw materials include: 28%~32% of pine cone bark, 18%~22% of peanut shell powder, 4%~6% of soybean meal, 4%~6% of chicken blood powder, 8%~12% of bean dregs, 8%~12% of soybean residue, 0.55%~0.65% of illite, 8%~12% of Bacillus subtilis, 0.0004%~0.0006% of Shuangxin oligofertilizer, and water.

6. The preparation method according to claim 5, wherein The raw materials include: 30% pine cone bark, 20% peanut shell powder, 5% soybean meal, 5% chicken blood powder, 10% bean dregs, 10% soybean residue, 10% soybean dregs, 10% soybean paste residue, 10% Bacillus subtilis, 0.6% illite, 0.0005% Shuangxin oligofertilizer, and water.

7. The preparation method according to any one of claims 4 to 6, characterized in that The conditions for biological fermentation include: temperature 30°C~35°C, humidity 55%~60%, and pH 6.5~7.

0.

8. The preparation method according to any one of claims 4 to 6, characterized in that The fermentation of the pine cone bark comprises: Mix unfermented pine cone bark with soil and organic matter, and control the fermentation conditions to 50% to 60% humidity, 25°C to 30°C, and ferment for 3 to 6 months; The relative amounts of the unfermented pine cone bark, the soil and the organic matter are (6-7):(1-2):(3-4); and the organic matter includes one or more of bean cake, sawdust and dead leaves.

9. A blast-proof and disease-resistant probiotic fermented feed prepared by the preparation method according to any one of claims 4 to 8.

10. A method for raising non-human animals, characterized in that: The non-human animal is administered the anti-epidemic and anti-disease probiotic fermented feed according to claim 9.

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