Device and method for extracting active peptide from astragalus membranaceus
By combining low-temperature treatment and grinding disc rubbing, the extraction process of astragalus active peptides was optimized, the problem of high cellulose and lignin content in the rhizome of astragalus was solved, and the efficient extraction and enrichment of active peptides was achieved.
Patent Information
- Application Number
- CN202510795636.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-06-16
- Publication Date
- 2025-10-03
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The extraction of Astragalus active peptides is difficult in the prior art, especially due to the high content of cellulose and lignin in the rhizome, which leads to high extraction cost and low efficiency.
The root apex and fibrous roots of Astragalus membranaceus were treated at low temperature, rubbed with a grinding disc and rinsed with low-temperature water. Combined with low-temperature rinsing, centrifugation, ultrafiltration, gel filtration and other steps, the extraction process was optimized to improve the extraction efficiency of active peptides.
The efficient extraction of Astragalus active peptides was achieved, the extraction cost was reduced, and the enrichment effect of the active peptides was improved.
Smart Images
Figure CN120737142A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the field of active peptide biotechnology and relates to an extraction device and method for astragalus active peptides. Background Art
[0002] In the field of traditional Chinese medicine, Astragalus is an important Qi-tonifying medicine; in modern pharmacological research, Astragalus has the effects of enhancing immune kinetics and protecting the cardiovascular system.
[0003] In recent years, the production of Astragalus has been increasing year by year. Besides being a traditional Chinese medicine, Astragalus has also been developed into health products. Extracting Astragalus's active peptides to develop health foods and cosmetics has a broad market.
[0004] At present, the medicinal value of Astragalus is mainly in its rhizome, and the content of cellulose and lignin in the rhizome of Astragalus is high, that is, the content of active peptides is relatively low, and the active peptides are mostly wrapped by the plant cell walls, which makes extraction difficult. If conventional active peptide extraction methods are used, the cost is too high and the efficiency is low. Summary of the Invention
[0005] In order to overcome the defects in the above-mentioned related technologies, the present invention provides a method for extracting active peptides from Astragalus membranaceus.
[0006] To achieve the above technical objectives, the method for extracting the active peptides from Astragalus membranaceus of the present invention comprises: Obtaining active roots of Astragalus membranaceus and washing them to remove soil from the roots; cutting the main root, the root tip region of the lateral root and the fibrous root of the astragalus; The cut root tip and fibrous roots are subjected to low temperature treatment to maintain the temperature of the root tip and fibrous roots between -5°C and 0°C; The root apex and fibrous roots are rubbed with a grinding disc, the surface temperature of the grinding disc being 0°C to 4°C, and the rubbed root apex and fibrous root surfaces are rinsed with low-temperature water to obtain a rinse solution; Rinse the rubbed root tip and fibrous roots in low-temperature water to obtain a rinsing solution; After mixing the flushing solution and the rinsing solution, the mixture was centrifuged at 4°C and 8000 rpm for 20 min to obtain the centrifuge solution; Adjusting the pH of the centrifuge to neutral; Select a hollow fiber ultrafiltration membrane with a molecular weight cutoff of 3kDa~10kDa, and ultrafilter the centrifuge at a pressure of 0.1MPa~0.2MPa and 4°C; Ammonium sulfate was added to the solution obtained after ultrafiltration to 30% saturation, and the solution was allowed to stand at 4°C for 1 hour, centrifuged at 10,000 rpm for 15 minutes, and the supernatant was collected; The supernatant was desalted and subjected to gel filtration chromatography, and the organic solvent was removed at low temperature.
[0007] Preferably, the method of performing gel filtration chromatography after desalting the supernatant comprises: using Sephadex G-25 or G-50 medium and Tris-HCl buffer as mobile phases in the gel filtration chromatography.
[0008] Preferably, the method of performing low-temperature treatment on the cut root apex and fibrous roots comprises: The cut root tip and fibrous roots were placed in distilled water at 4°C for 2-3 hours; The root apex, fibrous roots and distilled water are placed in a temperature environment of -5°C to 0°C to form ice crystals on the surface of the root apex and fibrous roots.
[0009] Preferably, the temperature of the low-temperature water used to rinse the rubbed root tip area and the fibrous root surface is 0-4°C.
[0010] On the other hand, the present invention also provides an extraction device for astragalus active peptides, which is suitable for the above-mentioned extraction method for astragalus active peptides.
[0011] The extraction device of the active peptide of Astragalus membranaceus comprises: a bracket, a first grinding disc and a second grinding disc. The bracket is fixed relative to the ground. The first grinding disc is arranged on the bracket, and the first grinding disc comprises: a disc body and an anti-slip ring. The disc body is a metal circular plate. A first central axis is fixed on one side of the disc body. The first central axis is connected to the bracket through a bearing. The anti-slip ring is a cylindrical shell. A plurality of through holes are provided on the side wall of the anti-slip ring. The anti-slip ring is fixed to the other side of the disc body. The second grinding disc is arranged on the bracket, and the second grinding disc is a metal circular plate. A second central axis is fixed on one side of the second disc. The second central axis is connected to the bracket through a bearing. The second grinding disc is adapted to the anti-slip ring of the structure. The second grinding disc is arranged relative to the disc body, and the second grinding disc is movably arranged in the anti-slip ring.
[0012] Preferably, the bracket comprises: A fixed frame, the fixed frame is a square frame, and the first grinding disc is arranged on the fixed frame; A track, the track is arranged horizontally, and the track and the fixing frame are fixedly arranged relative to each other; A movable frame, the movable frame is arranged on the track, and the movable frame is a square frame, and the second grinding disc is arranged on the movable frame; An electric push rod, wherein the housing of the electric push rod is fixedly arranged relative to the fixed frame, and the piston rod of the electric push rod is fixedly connected to the movable frame.
[0013] Preferably, the extraction device for the astragalus active peptides further comprises: a first motor, wherein the first motor is fixed to the fixing frame, and an output shaft of the first motor is connected to the first grinding disc; A second motor is fixed on the movable frame, and an output shaft of the second motor is connected to the second grinding disc.
[0014] Preferably, the extraction device for the astragalus active peptide further comprises a pressure sensor, and the pressure sensor is fixed on the second central axis.
[0015] Preferably, a water outlet nozzle is provided on the fixing frame above the anti-slip ring, and a plurality of water outlet nozzles are directed toward the anti-slip ring; A first temperature sensor is provided on the side of the disc body close to the second grinding disc, and a second temperature sensor is provided on the side of the second grinding disc close to the first grinding disc.
[0016] Preferably, the extraction device for the astragalus active peptide further comprises a microprocessor, and the microprocessor is electrically connected to the first motor, the second motor, the pressure sensor, the first temperature sensor, the second temperature sensor and the electric push rod.
[0017] The beneficial effects of the present invention are: The present invention adopts the root tip area and fibrous roots of Astragalus membranaceus, keeps them fresh at below zero degrees, and then rubs them with a grinding disc. Meanwhile, the kneading is rinsed with clean water above zero degrees, which can accelerate the rupture of plant cell walls and realize the large-scale separation of cellulose and lignin, thereby providing a concentrated solution for the subsequent extraction of active peptides and realizing the enrichment of trace active peptides. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or related technologies, the following briefly introduces the drawings required for use in the embodiments or related technical descriptions. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.
[0019] Figure 1 It is a structural diagram of the present invention. DETAILED DESCRIPTION
[0020] To make the above-mentioned objects, features, and advantages of the present invention more clearly understood, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.
[0021] In the description of the present invention, it should be understood that the terms "center", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention.
[0022] The terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the quantity of the technical features being referred to. Thus, a feature specified as "first" or "second" may explicitly or implicitly include one or more of such features. In the description of the present invention, unless otherwise specified, "plurality" means two or more.
[0023] The invention provides a method for extracting astragalus active peptides.
[0024] To achieve the above technical objectives, the method for extracting the active peptides from Astragalus membranaceus of the present invention comprises: Obtaining active roots of Astragalus membranaceus and washing them to remove soil from the roots; cutting the main root, the root tip region of the lateral root and the fibrous root of the astragalus; The cut root tip and fibrous roots are subjected to low temperature treatment to maintain the temperature of the root tip and fibrous roots between -5°C and 0°C; The root apex and fibrous roots are rubbed with a grinding disc, the surface temperature of the grinding disc being 0°C to 4°C, and the rubbed root apex and fibrous root surfaces are rinsed with low-temperature water to obtain a rinse solution; Rinse the rubbed root tip and fibrous roots in low-temperature water to obtain a rinsing solution; After mixing the flushing solution and the rinsing solution, the mixture was centrifuged at 4°C and 8000 rpm for 20 min to obtain the centrifuge solution; Adjusting the pH of the centrifuge to neutral; Select a hollow fiber ultrafiltration membrane with a molecular weight cutoff of 3kDa~10kDa, and ultrafilter the centrifuge at a pressure of 0.1MPa~0.2MPa and 4°C; Ammonium sulfate was added to the solution obtained after ultrafiltration to 30% saturation, and the solution was allowed to stand at 4°C for 1 hour, centrifuged at 10,000 rpm for 15 minutes, and the supernatant was collected; The supernatant was desalted and subjected to gel filtration chromatography, and the organic solvent was removed at low temperature.
[0025] Preferably, the method of performing gel filtration chromatography after desalting the supernatant comprises: using Sephadex G-25 or G-50 medium and Tris-HCl buffer as mobile phases in the gel filtration chromatography.
[0026] Preferably, the method of performing low-temperature treatment on the cut root apex and fibrous roots comprises: The cut root tip and fibrous roots were placed in distilled water at 4°C for 2-3 hours; The root apex, fibrous roots and distilled water are placed in a temperature environment of -5°C to 0°C to form ice crystals on the surface of the root apex and fibrous roots.
[0027] Preferably, the temperature of the low-temperature water used to rinse the rubbed root tip area and the fibrous root surface is 0-4°C.
[0028] On the other hand, Figure 1 As shown, the present invention also provides an extraction device for astragalus active peptides, which is suitable for the above-mentioned extraction method for astragalus active peptides.
[0029] The extraction device of the active peptide of Astragalus membranaceus comprises: a bracket 1, a first grinding disc 2 and a second grinding disc 3. The bracket 1 is fixed relative to the ground. The first grinding disc 2 is arranged on the bracket 1, and the first grinding disc 2 comprises: a disc body 21 and an anti-slip ring 22. The disc body 21 is a metal circular plate. A first central axis is fixed to one side of the disc body 21. The first central axis is connected to the bracket 1 through a bearing. The anti-slip ring 22 is a cylindrical shell. A plurality of through holes are provided on the side wall of the anti-slip ring 22. The anti-slip ring 22 is fixed to the other side of the disc body 21. The second grinding disc 3 is arranged on the bracket 1. The second grinding disc 3 is a metal circular plate. A second central axis is fixed to one side of the second disc. The second central axis is connected to the bracket 1 through a bearing. The second grinding disc 3 is adapted to the anti-slip ring 22 of the structure. The second grinding disc 3 is arranged relative to the disc body 21, and the second grinding disc 3 is movably arranged in the anti-slip ring 22.
[0030] Preferably, the bracket 1 comprises: A fixed frame, which is a square frame, and the first grinding disc 2 is arranged on the fixed frame; A track, the track is arranged horizontally, and the track and the fixing frame are fixedly arranged relative to each other; A movable frame is provided on the track and is a square frame, and the second grinding disc 3 is provided on the movable frame; An electric push rod, wherein the housing of the electric push rod is fixedly arranged relative to the fixed frame, and the piston rod of the electric push rod is fixedly connected to the movable frame.
[0031] Preferably, the extraction device for the astragalus active peptides further comprises: a first motor 4, wherein the first motor 4 is fixed to the fixing frame, and an output shaft of the first motor 4 is connected to the first grinding disc 2; The second motor 5 is fixed on the movable frame, and the output shaft of the second motor 5 is connected to the second grinding disc 3 .
[0032] Preferably, the extraction device for the astragalus active peptide further comprises a pressure sensor, and the pressure sensor is fixed on the second central axis.
[0033] Preferably, a water outlet nozzle is provided on the fixing frame above the anti-slip ring 22, and a plurality of water outlet nozzles are directed toward the anti-slip ring 22; A first temperature sensor is provided on the side of the disc body 21 close to the second grinding disc 3 , and a second temperature sensor is provided on the side of the second grinding disc 3 close to the first grinding disc 2 .
[0034] Preferably, the extraction device for the active peptides of Astragalus membranaceus further comprises a microprocessor, and the microprocessor is electrically connected to the first motor 4, the second electric motor, the pressure sensor, the first temperature sensor, the second temperature sensor and the electric push rod.
[0035] The specific operation process of this application is as follows: A certain amount of root apex and / or fibrous roots soaked in distilled water and with ice crystals formed on the surface are placed in the anti-slip ring 22, and the position of the second grinding disc 3 is adjusted so that the second grinding disc 3, the disc body 21, and the anti-slip area form a rubbing space. The disc body 21 rotates in the opposite direction relative to the second grinding disc 3. At the same time, the distance between the disc body 21 and the second grinding disc 3 is gradually reduced, and a water nozzle is used to continuously spray the rubbed root apex and / or fibrous roots.
[0036] When rubbing, the cell wall rupture of the root tip and / or fibrous roots can be accelerated. At the same time, rubbing can make the problem suddenly rise. When the intracellular liquid transforms from ice crystals to liquid, the cell wall rupture can be promoted, thereby accelerating the separation of cell fluid rich in active peptides from cellulose and lignin.
[0037] In addition, the first temperature sensor and the second temperature sensor are used to monitor the temperature during kneading to prevent excessive temperature from damaging the activity.
[0038] In the description of this specification, specific features, structures, materials or characteristics may be combined in an appropriate manner in any one or more embodiments or examples.
[0039] The above are only specific embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any changes or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in this invention should be included in the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be based on the scope of protection of the claims.
Claims
1. A method for extracting active peptides from Astragalus membranaceus, characterized in that: include: Obtaining active roots of Astragalus membranaceus and washing them to remove soil from the roots; cutting the main root, the root tip region of the lateral root and the fibrous root of the astragalus; The cut root tip and fibrous roots are subjected to low temperature treatment to maintain the temperature of the root tip and fibrous roots between -5°C and 0°C; The root apex and fibrous roots are rubbed with a grinding disc, the surface temperature of the grinding disc being 0°C to 4°C, and the rubbed root apex and fibrous root surfaces are rinsed with low-temperature water to obtain a rinse solution; Rinse the rubbed root tip and fibrous roots in low-temperature water to obtain a rinsing solution; After mixing the flushing solution and the rinsing solution, the mixture was centrifuged at 4°C and 8000 rpm for 20 min to obtain the centrifuge solution; Adjusting the pH of the centrifuge to neutral; Select a hollow fiber ultrafiltration membrane with a molecular weight cutoff of 3kDa~10kDa, and ultrafilter the centrifuge at a pressure of 0.1MPa~0.2MPa and 4°C; Ammonium sulfate was added to the solution obtained after ultrafiltration to 30% saturation, and the solution was allowed to stand at 4°C for 1 hour, centrifuged at 10,000 rpm for 15 minutes, and the supernatant was collected; The supernatant was desalted and subjected to gel filtration chromatography, and the organic solvent was removed at low temperature.
2. The method for extracting the active peptides from Astragalus membranaceus according to claim 1, wherein The method of performing gel filtration chromatography after desalting the supernatant comprises: using Sephadex G-25 or G-50 medium and Tris-HCl buffer as mobile phases in the gel filtration chromatography.
3. The method for extracting the active peptides from Astragalus membranaceus according to claim 2, wherein The method for performing low-temperature treatment on the cut root apex and fibrous roots comprises: The cut root tip and fibrous roots are placed in distilled water at a temperature of 4° C. for 2 h to 3 h; The root apex, fibrous roots and distilled water are placed in a temperature environment of -5°C to 0°C to form ice crystals on the surface of the root apex and fibrous roots.
4. The method for extracting the active peptides from Astragalus membranaceus according to claim 3, wherein The temperature of the low-temperature water used for washing the rubbed root tip area and the surface of the fibrous roots is 0-4°C.
5. An extraction device for active peptides from Astragalus membranaceus, suitable for the extraction method for active peptides from Astragalus membranaceus according to claims 1 to 4, characterized in that: The extraction device of the astragalus active peptide comprises: A bracket, wherein the bracket is fixed relative to the ground; A first grinding disc, the first grinding disc is arranged on the bracket, and the first grinding disc comprises: a disc body and an anti-slip ring, the disc body is a metal circular plate, a first central shaft is fixed to one side of the disc body, the first central shaft is connected to the bracket through a bearing, the anti-slip ring is a cylindrical shell, a plurality of through holes are provided on the side wall of the anti-slip ring, and the anti-slip ring is fixed to the other side of the disc body; The second grinding disc is arranged on the bracket, the second grinding disc is a metal circular plate, a second center shaft is fixed on one side of the second disc, the second center shaft is connected to the bracket through a bearing, the second grinding disc is adapted to the anti-slip ring of the structure, the second grinding disc is arranged opposite to the disc body, and the second grinding disc is movably arranged in the anti-slip ring.
6. The extraction device for active peptides from Astragalus membranaceus according to claim 5, characterized in that: The bracket comprises: A fixed frame, the fixed frame is a square frame, and the first grinding disc is arranged on the fixed frame; A track, the track is arranged horizontally, and the track and the fixing frame are fixedly arranged relative to each other; A movable frame, the movable frame is arranged on the track, and the movable frame is a square frame, and the second grinding disc is arranged on the movable frame; An electric push rod, wherein the housing of the electric push rod is fixedly arranged relative to the fixed frame, and the piston rod of the electric push rod is fixedly connected to the movable frame.
7. The extraction device for active peptides from Astragalus membranaceus according to claim 6, characterized in that: The extraction device of the astragalus active peptide also includes: a first motor, wherein the first motor is fixed to the fixing frame, and an output shaft of the first motor is connected to the first grinding disc; A second motor is fixed on the movable frame, and an output shaft of the second motor is connected to the second grinding disc.
8. The extraction device for active peptides from Astragalus membranaceus according to claim 7, characterized in that: The device for extracting astragalus active peptides further includes a pressure sensor, which is fixed on the second central axis.
9. The extraction device for active peptides from Astragalus membranaceus according to claim 8, characterized in that: A water outlet nozzle is provided on the fixing frame above the anti-slip ring, and a plurality of water outlet nozzles are directed toward the anti-slip ring; A first temperature sensor is provided on the side of the disc body close to the second grinding disc, and a second temperature sensor is provided on the side of the second grinding disc close to the first grinding disc.
10. The extraction device for active peptides from Astragalus membranaceus according to claim 9, characterized in that: The extraction device for the active peptides of Astragalus membranaceus further comprises a microprocessor, which is electrically connected to the first motor, the second motor, the pressure sensor, the first temperature sensor, the second temperature sensor and the electric push rod.