Yellow morchella strain ZJHYDJ001 with high iron content and application of yellow morchella strain ZJHYDJ001

Abstract

The invention discloses a yellow morchella strain ZJHYDJ001 with high iron content and application of the yellow morchella strain ZJHYDJ001, and relates to the technical field of microorganisms, and the yellow morchella strain ZJHYDJ001 has the preservation number of CGMCC No.41871. The Morchella sp. Is classified and named as Morchella sp. The average yield per square meter of the strain is 0.552 kg, and the average yield per mu can reach 276 kg; by adjusting the picking time, the sporocarp which is larger in stipe and cap size and suitable for the dried product can be obtained, the cap length of the obtained sporocarp can reach 71.86 mm, the stipe length can reach 90.66 mm, and the overall weight of a single sporocarp can reach 34.6 g.

Description

Technical Field

[0001] The present application relates to the field of microbial technology, and in particular to a high-iron-content Morchella fumigatus strain ZJHYDJ001 and its application. Background Art

[0002] Morel Morel ) belongs to the phylum Ascomycota ( Ascomycota )、Petrinomycetes( Pezizomycetes )、Morelaceae( Morchellaceae ), Morel ( Morchella ), is a kind of rare edible and medicinal fungi distributed worldwide. It has the effects of benefiting the stomach and intestines, aiding digestion, resolving phlegm and regulating qi, nourishing the kidneys and absorbing qi, nourishing the brain and refreshing the mind. It also has antioxidant, antibacterial, antiviral, immune regulation and liver protection functions.

[0003] Indoor cultivation of morels was first achieved in the late 20th century, using the red morel variety. Since 2012, my country has commercially cultivated black morels, covering most of the country. Currently, the main varieties of morels cultivated in China are the ladder-shaped morel (M. importuna), the sixth-sister morel (M. sextelata), and the seventh-sister morel (M. eximia).

[0004] Commercially cultivated Morchella cultivars all belong to the black Morchella lineage and are ecologically typical burnt land varieties. In addition, Mel-9, Mel-13, M. owneri, and Mes-21 have also been reported to be able to produce mushrooms. However, the yellow Morchella ( Morchella sp. Despite attempts at field, forest, and indoor biomimetic domestication cultivation, existing varieties of Morchella edulis suffer from fruiting body distortions of up to 80% when cultivated in artificial fields, preventing the stable inheritance of the beneficial mycelial traits. Furthermore, existing varieties of Morchella edulis in artificial field cultivation suffer from low yields, high rates of fungal contamination, small fruiting bodies with short caps, low-grade dried fruiting bodies, and overall short fruiting body length.

[0005] Wild yellow morels are primarily found in ditches, embankments, orchards, tea plantations, fields, roadsides, grasslands, and woodlands, and are rarely found in burned areas. Currently cultivable yellow and traditional black varieties lack the ability to withstand high temperatures. During artificial cultivation, greenhouse temperature and humidity control is necessary to meet these requirements. However, this regulation is often ineffective in some areas, resulting in frequent crop failures of cultivated yellow morels in these areas, impacting the yield of cultivated yellow morels.

[0006] The information disclosed in the background section is only intended to enhance understanding of the overall background of the invention and should not be considered as an admission or any form of suggestion that the information constitutes the prior art already known to a person of ordinary skill in the art. Summary of the Invention

[0007] In response to the above-mentioned technical problems, the present application provides a high-iron yellow morel strain ZJHYDJ001 and its application. The average yield of this strain is 0.552 kg per square meter, and the average yield per mu can reach 276 kg. By adjusting the picking time, fruiting bodies with larger stipe and cap sizes suitable for dried products can also be obtained. The cap length of the obtained fruiting body can reach 71.86 mm, the stipe length can reach 90.66 mm, and the overall weight of a single fruiting body can reach 34.6 g.

[0008] The present application provides a high iron content yellow morel strain ZJHYDJ001 and its application, with a deposit number of CGMCC No. 41871; a classification name of Morchella Morchella sp. .

[0009] Preferably, the iron content of the fruiting body of Morchella flavescentis strain ZJHYDJ001 is 659 mg / kg, and the average yield per mu is 276 kg.

[0010] Preferably, the cap length of the fruiting body of Morchella flavescentis strain ZJHYDJ001 is 50.46-71.86 mm, the stipe length is 47.09-90.66 mm, and the total weight of a single fruiting body is 17.74-34.6 g.

[0011] Another aspect of the present application also provides the use of the above-mentioned high-iron Morchella flavescentis strain ZJHYDJ001 in artificial field cultivation.

[0012] Preferably, in artificial field cultivation, when the fruiting body grows to maturity, the obtained fresh fruiting body has a stipe diameter of 11.13 mm, a stipe length of 47.09 mm, a weight of 17.74 g, a cap length of 50.46 mm, and a diameter of 38.17 mm at the widest part of the cap.

[0013] Preferably, in artificial field cultivation, the fruiting bodies are harvested 3 to 7 days after they grow to maturity, and the obtained fruiting bodies have a stipe diameter of 24.39 mm, a stipe length of 90.66 mm, a weight of 34.6 g, a cap length of 71.86 mm, and a diameter of 44.54 mm at the widest part of the cap.

[0014] Preferably, in artificial field cultivation, after the primordium is grown, the strain can tolerate an ambient temperature of 24-29.5°C in the greenhouse for one month continuously, and when harvested, there are no dead mushrooms, mushrooms killed by disease infection, mushrooms with deformities, or mushrooms infected with white mold.

[0015] Preferably, the artificial field cultivation comprises the following steps: breaking the cultivated seeds covered with mycelium into pieces and evenly spreading them on the pre-treated, deep-ploughed and sealed greenhouse floor, and covering with soil to complete sowing; placing nutrient bags in a triangular shape on the floor 10 days after sowing, covering with film, watering to promote mushroom production, and cultivating the mushrooms at 15-25°C and an air humidity of 85% for 15-25 days before the fruiting bodies enter the mature stage and are harvested.

[0016] Preferably, the preparation of the cultivar comprises the following steps: Inoculate the mother strain into a cultivation bottle filled with cultivation material at a rate of 1 block / bag, and cultivate in the dark at 19°C until the mixture is covered with mycelium to obtain the original strain; The original seed is inoculated into a cultivation bag filled with cultivation material at a rate of 10-30 g / bag and cultured in the dark at 19°C until the mycelium fills the cultivation bag, thereby obtaining a cultivated seed. Preferably, the cultivation material comprises 51.5 g of wheat, 45 g of sawdust, 1.5 g of lime, 1 g of gypsum, and 1 g of potassium dihydrogen phosphate, with a moisture content of 60%.

[0017] The percentages used in the present invention are all mass percentages.

[0018] The beneficial effects of this application include: 1) This application provides the high-iron Morchella yellow strain ZJHYDJ001 and its applications. The Mes-15 strain ZJHYDJ001 has an iron content of up to 659 mg / kg, significantly higher than the iron content of existing Morchella varieties (which reaches a maximum of only 168.2 mg / kg). This strain possesses a remarkable high iron content, which facilitates the human body's iron intake from food. This effectively compensates for the low iron content of existing Morchella varieties. This strain has an average yield of 0.552 kg per square meter, and an average yield of 276 kg per mu. By adjusting the harvesting timing, fruiting bodies with larger stipe and cap sizes suitable for dried products can be obtained. The resulting fruiting bodies have caps up to 71.86 mm and stipes up to 90.66 mm in length, and a single fruiting body weighs up to 34.6 g. This significantly increases yield. Mature fruiting bodies can reach a length of up to 19.3 cm when dried, and the resulting fruiting bodies are of high quality, considered premium quality.

[0019] 2) The high-iron Morchella strain ZJHYDJ001 provided in this application features a pale yellow cap after mature fruiting, a thin and short stipe, a strong aroma, and strong mycelial resistance to foreign bacteria. ZJHYDJ001 also boasts early fruiting, high-temperature tolerance, and high yields during fruiting. During the fruiting season, with an average temperature of 26.4°C and a maximum temperature of 29.5°C, ZJHYDJ001 exhibited no dead mushrooms, disease, white mold, or deformities.

[0020] Morchella yellow strain ( Morchella sp. (Mes-15)) ZJHYDJ001, deposited at China General Microbiology Center (CGMCC), address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, deposit date: March 31, 2025, deposit number: CGMCC No. 41871; suggested taxonomic name: Morchella Morchella sp. . BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a schematic diagram of the developmental tree of the Morchella flavescentis strain ZJHYDJ001 obtained in Example 1 provided in this application; Figure 2 The mycelium and cultivation bag photos of the yellow morel strain ZJHYDJ001 strain cultivated in Example 7 and Example 4 provided in this application, wherein Figure 2 a is a photo of mycelium and sclerotia obtained by culture in a culture dish in Example 7; Figure 2 b is a real photo of the cultivation bag; Figure 3 This is a photo of the mature fruiting body of the yellow morel strain ZJHYDJ001 cultivated in the artificial field in Example 4 provided in this application, wherein Figure 3 a~d are photos taken from different angles of mature fruiting bodies in the field before harvesting; Figure 4 Actual photos of the fruiting bodies of the yellow morel strain ZJHYDJ001 at different stages of artificial field cultivation in Example 4 provided in this application; the fruiting bodies in the figure are harvested from the time of fruiting to the maturity period, and samples are taken every 3 days; the fruiting bodies in the mature period can reach a length of 19.3 cm after being made into dried products. DETAILED DESCRIPTION

[0022] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments, but the present invention is not limited in any way. Any changes or improvements made based on the teachings of the present invention fall within the scope of protection of the present invention.

[0023] Unless otherwise specified, the materials and instruments used in the following examples were obtained from commercial channels; the detection methods used were all existing methods unless otherwise specified.

[0024] The enriched medium used in the following examples is: 20 g / L glucose, 150 g / L potato juice, 1.5 g / L peptone, 2.5 g / L beef extract, 1.5 g / L potassium dihydrogen phosphate, 0.7 g / L magnesium sulfate, and 17 g / L agar (agar is selected depending on whether a solid medium is required), with a natural pH value.

[0025] Example 1 Acquisition and Identification of Morchella Flavescentis Strain ZJHYDJ001 1. Collection: Zhang Junbo collected wild-growing yellow morel fruiting bodies from the urban sewers in Kunming, Yunnan Province on May 18, 2023; (1) Tissue separation and screening: Inoculate the tissue blocks of the fruiting body through a slant test tube with enriched culture medium in a super-bacterial table, culture in the dark at 19°C for 7 days, and then purify. Continue to culture for 7 days to select the test tube with better growth, pick the strain in the test tube, and make the culture strain. The culture strain is inoculated at an inoculum of 20-30 grams into a cultivation bag containing 185 grams of bagged cultivation material. The cultivation material used is composed of 51.5 grams of wheat, 45 grams of sawdust, 1.5 grams of lime, 1 gram of gypsum, and 1 gram of potassium dihydrogen phosphate. "Good growth" means that the mycelium is thick and vigorous, and the sclerotia are abundant. (2) Cultivate at 19°C until the mycelium fills the bag, transplant to the field and cultivate until the mushrooms are produced, select the fruiting bodies of the yellow morel with better fruiting body characteristics, pick them and repeat the above-mentioned tissue separation process to obtain the strain, prepare the obtained strain into an inoculum according to the existing method, inoculate it again in the cultivation bag, cultivate under normal management until the mushrooms are produced, and harvest the fruiting bodies; better fruiting body characteristics refer to: the fruiting body has the best flower shape, the heaviest weight, and the most uniform color.

[0026] By repeating steps (1) to (2) for fruiting and breeding, a yellow morel strain ZJHYDJ001 with high fruiting yield and good disease resistance was finally obtained.

[0027] The yellow morel strain ZJHYDJ001 was inoculated into a slant test tube with enriched medium and cultured in the dark at 19°C for 7 days. Figure 2 As shown, the mycelial blocks were cut and placed in sterile distilled water freezing tubes and stored in a 4°C refrigerator.

[0028] 2. Identification of Morchella flavescentis strain ZJHYDJ001 DNA from mycelia and fruiting bodies of ZJHYDJ001 was extracted by CTAB method, and the DNA was detected by 1% agarose gel electrophoresis and GelRed staining. The extracted total DNA was used as template and the ITS and LSU sequences were amplified using Mix reagent (Hunan Qingke Biotechnology Co., Ltd.) with primers ITS4 (5'-TCCTCCGCTTATTGATATGC-3'), ITS5 (5'-GGAAGTAAAAGTCGTAACAAGG-3'), LR0R (5'-ACCCGCTGAACTTAAGC-3'), and LR5 (5'-TCCTGAGGGAAACTTCG-3').

[0029] The PCR amplification system (25 μL) consisted of 12.5 μL of 2× Mix, 0.5 μL of each 10 μM primer, 11 μL of ddH2O (redistilled water), and 0.5 μL of DNA template. The reaction procedure was: pre-denaturation at 95°C for 5 min, followed by 35 cycles of denaturation at 95°C for 40 sec, annealing at 50°C for 40 sec, extension at 72°C for 1 min, and then extension at 72°C for 10 min. The amplified DNA was then analyzed by electrophoresis on a 1% agarose gel and sent to Qingke Biotechnology for sequencing.

[0030] After bidirectional splicing of the sequencing results of ZJHYDJ001 mycelium and cultivated fruiting bodies, the obtained ZJHYDJ001-mycelium ITS sequence was 1080 bp in length (such as SEQ ID: No. 1) and the LSU sequence was 894 bp in length (such as SEQ ID: No. 2); the cultivated fruiting body ITS sequence was 1026 bp in length (such as SEQ ID: No. 3) and the LSU sequence was 848 bp in length (such as SEQ ID: No. 4).

[0031] SEQ ID: No. 1 (ZJHYDJ001-hypha ITS sequence) taacggtgga agacgccgtc atataggcgg gttccataac cggcacaaga tgttggaagg 60 gcccagggcg ggccggccgg gtcaacctca tccgcgtaat cctgctgccc actgcgctcc 120 ctccccacgc tttagggcag caaccccccg cattgggggg tggagccgga tctaagcaat 180 caatgcttcg catccatcca caacacagtt gggtgccggt gcggagtaga ctattgcggc 240 taaaggaggt tcacccggat ggaggctgac tgcgcatgca ggaccatcat ggatggattc 300 caaccatgcg ttccttcccc tggggcctga ggggtgtaaa aacccctcag cgacctgctc 360 cgacggcgtg aagagccc ccaccgtttg gcaccctctc gccattgtcc caaccaaaac 420 cctctgtgta cccttccctg ttgcttcccc cgggcaactg gctccggcca gccgggggggg 480 agaaaccaag caaaaaccct tttcgcaaaa cagacgtctg aatgtaaaaa aacaaaaaac 540 aaaagttaaa actttcaaca acggatctct tggttcccac atcgatgaag aacgcagcga 600 aatgcgataa gtaatgtgaa ttgcagaatt cagtgaatca tcgaatcttt gaacgcacat 660 tgcgccctct ggtattccgg agggcatgcc tgttcgagcg tcataaatac cgctccccct 720 cggattgctt gcggtccctg gggggttctg gcaatgtgga ttccccccgt gctttgaggg 780 catgcgaacg ggctcccagt gctgaaagac ataatgttcc cagccgaaac cggtgattta 840 tttcatcggc aggattcgtg gcaggcagac tgagggcgtc aaccgtggag tcatgaggat 900 agaaacctcc ccctttgcaa gtaacattgc tctggcagtt agatctgcag gcccgccggt 960 ctggggatgg accctcccac tcgtaggcgt cacggccacg atagcgggcg ttaatggaa 1020 tccgatccgc ccctccccgg gtggttgaag atccttgtgg gctagcaacc cctaaacaca 1080 SEQ ID: No. 2 (ZJHYDJ001 - Hyphal LSU Sequence) cagaaccagc tactagatgg ttcgattagt ctttcgcccc tatacccaaa tttgacgatc 60 gatttgcacg tcagaaccgc tgcgagcctc caccagagtt tcctctggct tcaccctatt 120 caggcatagt tcaccatctt tcgggtccca acagctatgc tcctactcag atccttcaga 180 agacttcggg accggtcgat ggtgcaccct tgcgggttcc cacctccgtt cactttcatt 240 tcgcgtaagg gtttgacacc caaacactcg catagatgtt agactccttg gtccgtgttt 300 caagacgggt cgctgaagac cattatgcca agcatcctag cccgaaggcg cggtcctcgg 360 tcggggctgg cggcattcac ccgggctata acactccccg aaaggagcca cattcccggg 420 acctttatcc cgccgtccca accgatgctg gcccggaggg gagcaagtgc accgcccaga 480 aggacgactg atcactcccc accgaagtct ggtctcaagc gcttcccttt caacaatttc 540 acgtactttt taactctatt ttcaaagtgc ttttcatctt tcgatcactc tacttgtgcg 600 ctatcggtct cccaccaata tttagcttta gatgaaattt accacccatt ttgagctgca 660 ttcccaaaca actcgactcg tcgaagacac ctcacatgga cggggacagc cagccaagca 720 cgggattctc accctctatg acgtcctgtt ccaaggaact taggccggcg ccctacccgg 780 agatgcctca caaaattaca acgcggacac cgggggtgcc agctttaaaa tttgagcttt 840 tgccgcttca ctcgccgtta ctgaggcaat ccctgttggt ttcttttcct ccgc 894 SEQ ID:No.3 (ZJHYDJ001 - ITS sequence of fruiting body) gaagacgccg tcatataggc gggttccata accggcacaa gatgttggaa gggcccaggg 60 cgggccggcc gggtcaacct catccgcgta atcctgctgc ccactgcgct ccctccccac 120 gctttagggc agcaaccccc cgcattgggg ggtggagccg gatctaagca atcaatgctt 180 cgcatccatc cacaacacag ttgggtgccg gtgcggagta gactattgcg gctaaaggag 240 gttcacccgg atggaggctg actgcgcatg caggaccatc atggatggat tccaaccatg 300 cgttccttcc cctggggcct gaggggtgta aaaacccctc agcgacctgc tccgacggcg 360 tgaagaggac ccccaccgtt tggcaccctc tcgccattgt cccaaccaaa accctctgtg 420 tacccttcc tgttgcttcc cccgggcaac tggctccggc cagccgggg ggagaaacca 480 agcaaaaacc cttttcgcaa aacagacgtc tgaatgtaaa aaaacaaaaa acaaaagtta 540 aaactttcaa caacggatct cttggttccc acatcgatga agaacgcagc gaaatgcgat 600 aagtaatgtg aattgcagaa ttcagtgaat catcgaatct ttgaacgcac attgcgccct 660 ctggtattcc ggagggcatg cctgttcgag cgtcataaat accgctcccc ctcggattgc 720 ttgcggtccc tggggggttc tggcaatgtg gattcccccc gtgctttgag ggcatgcgaa 780 cgggctccca gtgctgaaag acataatgtt cccagccgaa accggtgatt tatttcatcg 840 gcaggattcg tggcaggcag actgagggcg tcaaccgtgg agtcatgagg atagaaacct 900 ccccctttgc aagtaacatt gctctggcag ttagatctgc aggcccgccg gtctggggat 960 ggaccctccc actcgtaggc gtcacggcca cgatagcggg cgttaaatgg aatccgatcc 1020 gcccct 1026 SEQ ID: No.4 gaggttcgat tagtctttcg cccctatacc caaatttgac gatcgatttg cacgtcagaa 60 ccgctgcgag cctccaccag agtttcctct ggcttcaccc tattcaggca tagttcacca 120 tctttcgggt cccaacagct atgctcctac tcagatcctt cagaagactt cgggaccggt 180 cgatggtgca cccttgcggg ttcccacctc cgttcacttt catttcgcgt aagggtttga 240 cacccaaaca ctcgcataga tgttagactc cttggtccgt gtttcaagac gggtcgctga 300 agaccattat gccaagcatc ctagcccgaa ggcgcggtcc tcggtcgggg ctggcggcat 360 tcacccgggc tataacactc cccgaaagga gccacattcc cgggaccttt atcccgccgt 420 cccaaccgat gctggcccgg aggggagcaa gtgcaccgcc cagaaggacg actgatcact 480 ccccaccgaa gtctggtctc aagcgcttcc ctttcaacaa tttcacgtac tttttaactc 540 tattttcaaa gtgcttttca tctttcgatc actctacttg tgcgctatcg gtctcccacc 600 aatatttagc tttagatgaa atttaccacc cattttgagc tgcattccca aacaactcga 660 ctcgtcgaag acacctcaca tggacgggga cagccagcca agcacgggat tctcaccctc 720 tatgacgtcc tgttccaagg aacttaggcc ggcgccctac ccggagatgc ctcacaaaat 780 tacaacgcgg acaccggggg tgccagcttt aaaatttgag cttttgccgc ttcactcgcc 840 gttactga 848 Sequences were submitted to GeneBank for homology searches using BLAST (www.ncbi.nlm.nih.gov / BLAST) and analyzed for similarity with sequences from various strains in the database. Sequences were downloaded from the GeneBank database and identified using the multi-gene joint (ITS-LSU) matrix sequence analysis method. The ITS-LSU gene sequences of the most homologous species were aligned using the Clustal W function in MEGA6.0 software, and a phylogenetic tree was constructed using neighbor-joining (NJ). Default parameters were used, with 1000 bootstrap tests.

[0032] From the obtained multi-gene joint phylogenetic tree ( Figure 1 ), the hyphae and fruiting bodies of ZJHYDJ001 are clustered into one branch with the Morchella sp. species in the GeneBank database, indicating the stability of the strain's traits. The Morchella sp. species in the GeneBank database are clustered into one branch, so based on the results of BLAST and phylogenetic tree comparison, it can be concluded that the sequenced ZJHYDJ001 is a Morchella sp. (Mes-15).

[0033] The obtained strain ZJHYDJ001 was biologically deposited on March 31, 2025, with the deposit number CGMCC No. 41871; the proposed classification name is Morchella Morchella sp. .

[0034] Example 2 Nutrient Determination of Morchella sp. Mes-15 Strain ZJHYDJ001 The mature fruiting bodies of cultivated Morchella oleracea ZJHYDJ001 were harvested and dried, and samples were sent to the Kunming Edible Fungi Quality Supervision and Inspection Center of the China National Federation of Supply and Marketing Cooperatives for testing of some nutrients. The test results are shown in Table 1: Table 1: Some nutrients of Morchella oleracea ZJHYDJ001 Jia Hui, Fan Shangyi. Comparison of nutritional components of wild and cultivated Morchella in Gannan [J]. Journal of Gansu Normal University, 2023, 28(02): 20-23. The nutrient content of wild and cultivated Morchella is disclosed in the study. The iron content of each strain tested is relatively low, with the highest being only 168.2 mg / kg. However, the iron content of Morchella yellow ZJHYDJ001 can reach 659 mg / kg, which is much higher than that of existing Morchella varieties. This indicates that this strain has a significant high iron content, which is beneficial for the human body to absorb the iron required by the human body from food. This effectively compensates for the low iron content of existing Morchella varieties.

[0035] Example 3 Preparation of original seeds and cultivars (1) Preparation of mother culture: Take the mycelial block of ZJHYDJ001 strain obtained in Example 1 and store it in a refrigerator at 4°C. Take a piece of mycelial block and inoculate it on the slant of a slant test tube (test tube size is 18*18mm). The slant is enriched with culture medium. Incubate in the dark at 19°C until the mycelium covers the inner wall of the slant test tube to obtain the mother culture; (2) The formula of the cultivation material used for the original species and the cultivated species is: 51.5g wheat, 45g sawdust, 1.5g lime, 1g gypsum, and 1g potassium dihydrogen phosphate; soak the wheat in water one day in advance and soak overnight for later use; mix the raw materials according to the formula and stir evenly, add water to adjust the water content to 60% to obtain the cultivation material, bottle the obtained cultivation material, sterilize it at 121℃ for 120-150min, and cool it for later use; Inoculate the mother strain on the cooled culture medium in the bottle, take out the fungus blocks with a square block taker (with an inner cavity size of 1cm*1cm), and inoculate 1 block per bag. Cultivate in the dark at 19℃ until the mix is ​​covered with mycelium to obtain the original strain. After filling the cultivation bag with the cultivation material, sterilize it according to the above conditions and then cool it to obtain a cultivation bag. Inoculate the obtained original seed blocks into the cultivation bag at an inoculum rate of 10-30 grams per bag, and culture it in the dark at 19°C until the mycelium fills the cultivation bag to obtain the cultivated seeds.

[0036] Example 4 Field cultivation and yield statistics 1. Field cultivation: 1) Before sowing, pre-treat the soil and deep-plow it under a covered greenhouse to reduce soil bacteria and pests. Begin sowing preparations from September to December, when the soil temperature remains below 20°C. Add quicklime to the soil, till it evenly, and pre-wet it. Build beds with a width of 1-1.2 m and no length limit. 2) When sowing, break up the seeds (0.5 kg / m2) and spread evenly on the bed surface, then cover with 2-3 cm of soil. 3) 5-7 days after sowing, a large amount of hyphae and white frost will be visible on the surface of the soil. 10 days after sowing, begin placing nutrient bags (8-10 bags / m2, approximately 300 g per bag) in a triangular pattern on the bed surface. Make two parallel incisions about 3-5 cm on one side of the bed, buckle them to the bed surface, and cover with black mulch. Cultivate the fungi for 40-60 days. When the frost fades and a small amount of tangled particles are formed, and the soil temperature continues to rise, remove the mulch and water to promote mushroom growth. 4) During the fruiting period, the temperature in the greenhouse should be kept at 15-25℃ and the air humidity should be kept at 85%. The fruiting bodies should be harvested after 15-25 days of cultivation.

[0037] The above cultivation methods are only examples. The strain can also be cultivated in the field using any of the methods disclosed in Zhao Qi, Huang Yunting, Xu Zhongzhi, et al. Current status of research on morel cultivation [J]. Journal of Yunnan Agricultural University, 2009, 24(06): 904-907. DOI: 10.16211 / j.issn.1004-390x(n).2009.06.022.

[0038] See also Figure 3 It can be seen that the fruiting bodies obtained by cultivating the strain provided by this application using this method are similar in shape to wild Morchella; the fruiting bodies sampled every 3 days during the growth period after fruiting are as follows: Figure 4 , see Figure 4 The cap of the fruiting body after germination is black, gray when grown up, and yellow when old. The stipe is white and thin from young to mature, and becomes thicker, longer and wrinkled when old. The mature fruiting body can reach 19.3 cm in length after being made into dried products.

[0039] 2. Yield Statistics: Using the above cultivation method, 11 1m*1m square areas were artificially divided in the field. The fruiting bodies of Morchella in each area were harvested, and the weight of the fruiting bodies obtained in the area was weighed as the statistical yield. The results are shown in Table 2: Table 2: Yield statistics of Morchella oleracea ZJHYDJ001 The area of ​​a 1m*1m square area is 1 square meter, so the statistical yield result is the yield per square meter. The average of the 10 statistical results is obtained, and the average yield per square meter of yellow morel ZJHYDJ001 is 0.552kg. One mu is based on an effective area of ​​500m 2After conversion, the average yield per mu of the yellow morel ZJHYDJ001 can reach 276 kg. From the table, we can see that the maximum yield per square meter is 0.79 kg, the highest yield per mu is 395 kg, and the lowest yield per mu is 205 kg, with an average yield of over 200 kg per mu. At the current price of over 200 yuan per kilogram of yellow morels, the per mu income can reach 41,000 to 79,000 yuan, a considerable benefit.

[0040] Example 5 Analysis of Fruiting Body Traits of Morchella sp. Mes-15 Strain ZJHYDJ001 A cultivation experiment was conducted at the Kunming Edible Fungi Research Institute Cultivation Experiment Base in Jinning District, Kunming City, Yunnan Province, according to the method provided in Example 4. 100 mature and late fruiting bodies were randomly selected, and their cap diameter, cap length, stipe diameter, stipe length, and weight were measured. The average values ​​of each index were calculated and listed in Table 3. Late fruiting bodies refer to fruiting bodies that were picked 3 to 7 days after maturity.

[0041] Table 3: Average values ​​of fruiting body traits of Morchella oleracea ZJHYDJ001 From the above data, it can be seen that the stipe of mature fruiting bodies is thinner, and the diameter and length of the widest part of the cap are larger, which can effectively increase the proportion of the cap part of the fruiting body and help improve the grade of the obtained fruiting body.

[0042] While the cap size of mature fruiting bodies increases, the diameter and length of the stipe also increase significantly, resulting in a decrease in the proportion of the cap to the overall fruiting body. The cap color of mature fruiting bodies also changes significantly, becoming yellow as they age, and individual mushrooms become heavier. Mature fruiting bodies are suitable for fresh consumption, while older fruiting bodies are suitable for dried products. Harvesting the fruiting bodies of this strain can be adjusted according to production needs to achieve different product qualities.

[0043] Example 6 Detection of the High Temperature Resistance of Morchella sp. Mes-15 Strain ZJHYDJ001 in Cultivation Yellow Morchella ZJHYDJ001 cultivar and commercially available black Morchella cultivar were inoculated on the same growing medium under the same cultivation conditions and cultivated in the field. The cultivation method was the same as in Example 4. The field cultivation location was the Kunming Edible Fungi Research Institute base in Jinning District, Kunming City, Yunnan Province. The field cultivation was carried out in the same greenhouse, and the greenhouse area was divided into two equal parts: one was planted with yellow Morchella ZJHYDJ001 and the other was planted with commercially available black Morchella.

[0044] During the fruiting period in April 2025, the high temperature season, the temperature at evenly distributed temperature measuring points in the greenhouse was measured five times from 12:00 to 14:00 every day after the primordia grew out. The temperature measuring points were evenly distributed in the greenhouse, and any two temperature measuring points were more than 4 meters apart. The average temperature of the greenhouse on that day was calculated. The temperature values ​​were measured every day for 15 consecutive days. The temperature records are shown in Table 4: Table 4: Temperature during the 15-day fruiting period As can be seen from the above table, high temperature environment is more likely to appear in the greenhouse during the mushroom fruiting period in the high temperature season, and the temperature in the greenhouse can reach 29.5℃.

[0045] The number of primordiums, white mold, deformities, growth conditions, dead mushrooms, and diseases in each area were counted. The cultivation conditions in each area were counted separately, and the number of primordiums, white mold, deformities, dead mushrooms, and diseases of each fruiting body were checked one by one. The percentage = A / total number of fruiting bodies germinated in the transplanting area * 100%; A = the number of primordiums, the number of fruiting bodies with white mold, the number of fruiting bodies with deformities, the number of fruiting bodies with dead mushrooms, and the number of fruiting bodies with diseases. Even if no primordiums emerged, the statistics were still counted after normal planting. The results are listed in Table 5: Table 5: Statistical results of mushroom production Through records and observations, it can be seen that the yellow morel Morchella sp. Mes-15 strain ZJHYDJ001 has higher resistance to high temperatures when fruiting than the existing black morels. During the fruiting period in the high temperature season with an average temperature of 26.4℃ and a maximum temperature of 29.5℃, conventional black varieties will suffer from a decrease in the number of primary mushrooms, white mold on the top of the cap, a large number of diseases, growth deformities, poor growth and a large number of dead mushrooms under continuous high temperatures.

[0046] The Morchella edulis ZJHYDJ001 strain provided in this application, however, showed no signs of dead mushrooms, disease, white mold, or deformities. Therefore, Morchella edulis ZJHYDJ001 exhibits enhanced resistance to high temperatures and diseases during the fruiting stage, making it a high-quality Morchella edulis strain. This strain can grow at temperatures between 15°C and 28°C and is tolerant of moderate to high temperatures.

[0047] Example 7 Sclerotium count of Morchella sp. Mes-15 strain ZJHYDJ001 After the mother strain was obtained according to the method in Example 3, it was inoculated into a culture dish with enriched medium and cultured in the dark at 19°C until full of mycelium. The actual photo of the obtained culture dish is shown in the figure. Figure 2 As shown in a, Figure 2 It can be seen that the mycelium of strain ZJHYDJ001 is thick and the sclerotia are abundant, with the number of sclerotia reaching more than 100, indicating that the strain has strong growth activity.

[0048] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A yellow morel strain ZJHYDJ001 with high iron content, characterized in that: Deposit number: CGMCC No.41871; Classification name: Morchella Morchella sp. .

2. The yellow morel strain ZJHYDJ001 with high iron content according to claim 1, characterized in that The iron content of the fruiting body of Morchella yellow strain ZJHYDJ001 is 659 mg / kg, and the average yield per mu is 276 kg.

3. The yellow morel strain ZJHYDJ001 with high iron content according to claim 1, characterized in that The cap length of the fruiting body of the yellow morel strain ZJHYDJ001 is 50.46~71.86mm, the stipe length is 47.09~90.66mm, and the total weight of a single fruiting body is 17.74~34.6g.

4. Use of the high-iron yellow morel strain ZJHYDJ001 as claimed in claim 1 in artificial field cultivation.

5. The use according to claim 4, characterized in that In artificial field cultivation, when the fruiting bodies grow to maturity, the diameter of the fresh fruiting bodies is 11.13 mm, the length of the stipe is 47.09 mm, the weight is 17.74 g, the length of the cap is 50.46 mm, and the diameter of the widest part of the cap is 38.17 mm.

6. The use according to claim 4, characterized in that In artificial field cultivation, the fruiting bodies are harvested 3 to 7 days after they reach maturity. The resulting fruiting bodies have a stipe diameter of 24.39 mm, a stipe length of 90.66 mm, a weight of 34.6 g, a cap length of 71.86 mm, and a diameter of 44.54 mm at the widest part of the cap.

7. The use according to claim 4, characterized in that In artificial field cultivation, after the primordium grows out, the strain can tolerate the ambient temperature in the greenhouse at 24~29.5℃ for 1 month. When harvested, there are no dead mushrooms, mushrooms killed by disease infection, mushrooms with deformities, and mushrooms infected with white mold.

8. The use according to claim 4, characterized in that Artificial field cultivation includes the following steps: breaking the cultivated seeds covered with mycelium into pieces and evenly spreading them on the pre-treated, deep-ploughed and sealed greenhouse surface, and covering with soil to complete sowing; starting from 10 days after sowing, placing nutrient bags in a triangular shape on the greenhouse surface, covering with film, watering to promote mushroom production, and cultivating the mushrooms at 15-25℃ and 85% air humidity for 15-25 days. After the fruiting bodies enter the mature stage, they are harvested.

9. The use according to claim 8, characterized in that The preparation of cultivars comprises the following steps: Inoculate the mother strain into a cultivation bottle filled with cultivation material at a rate of 1 block / bag, and cultivate in the dark at 19°C until the mixture is covered with mycelium to obtain the original strain; The original seeds were inoculated into a cultivation bag filled with cultivation material at a rate of 10 to 30 g / bag and cultured in the dark at 19°C until the mycelium filled the cultivation bag to obtain cultivated seeds.

10. The use according to claim 4, characterized in that The cultivation material is 51.5g wheat, 45g sawdust, 1.5g lime, 1g gypsum, and 1g potassium dihydrogen phosphate, with a water content of 60%.

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