Bionic skin lipid and preparation method thereof

Abstract

The invention discloses bionic skin lipid and a preparation method thereof. The compound vegetable oil composed of the deep sea fish oil, the camellia seed oil and the prinsepia utilis royle oil is selected, and is rich in triacylglycerol, phytosphingosine, squalene, cholesteryl ester, cholesterol, wax ester, phospholipid and other lipids required by skin; candida lipolytica is adopted to ferment the compound vegetable oil, the bionic skin lipid is obtained after fermentation, the content of effective components and the use feeling are obviously improved, and the bionic skin lipid can be used as an active component to be added into a cosmetic matrix. The multi-dimensional effects of maintaining a skin barrier function, preserving moisture, resisting inflammation, regulating micro-ecology and the like are achieved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular to a kind of bionic skin lipid and preparation method thereof. BACKGROUND

[0002] With the popularization of science education, people gradually realize that skin care products are not just simple daily chemical industry products, but can interact with the skin to exert efficacy. Research on skin structure is increasing, from tissue to cell to molecular level, and the action mechanism of skin care product ingredients is systematically revealed.

[0003] The composition and content of skin lipids vary depending on the source, mainly including lipids secreted by sebaceous glands and intercellular lipids. The lipids secreted by sebaceous glands account for 75% to 85% of the total skin lipids, mainly composed of triacylglycerol, wax ester, squalene, cholesterol ester and cholesterol. Intercellular lipids account for 10% to 25% of the total skin lipids, mainly composed of ceramide, cholesterol, free fatty acid and phospholipid.

[0004] In recent years, scientific research has shown that skin lipids play a crucial role in maintaining skin health. They not only constitute the skin barrier, but also participate in skin moisturizing, repair and defense functions. When skin lipids are deficient, it can cause dry skin, damaged barrier, accelerated aging, increased risk of infection and other problems. Therefore, by using lipids-containing skin care products, paying attention to diet and environmental management, skin health can be effectively maintained, and skin problems can be prevented and improved. However, the currently marketed complex lipid products have the problems of single efficacy, poor absorption, etc., and cannot meet the needs of fine skin care. SUMMARY

[0005] One of the purposes of the present application is to provide a kind of bionic skin lipid, which plays a good skin care effect in maintaining skin barrier function, moisturizing, anti-inflammatory and regulating microecology.

[0006] In order to achieve the above purpose, the following technical means are adopted in the present application:

[0007] The present application provides a kind of bionic skin lipid, which is prepared by the following method:

[0008] S1, mix deep sea fish oil, camellia seed oil and Chinese pricklyash fruit oil to obtain an oil mixture;

[0009] S2, inoculate Candida lipolytica seed bacterial liquid into liquid culture medium for induced culture to obtain a culture solution;

[0010] S3, add the oil mixture to the culture solution for fermentation to obtain a fermentation solution;

[0011] S4, collecting the oil phase layer on the upper layer of the fermentation liquid, centrifuging and filtering, and obtaining a clear and sterile filtrate, which is the biomimetic skin lipid.

[0012] The deep-sea fish oil involved in this invention is extracted from the Atlantic snapper. Atlantic snapper, also known as the longevity fish or orange snapper, can live for over 100 years and is rich in over 20 essential trace elements, cholesterol esters, cholesterol, vitamins, and various amino acids. It also contains the valuable unsaturated fatty acid DHA, which can enhance human cell activity and boost immunity.

[0013] The camellia seed oil involved in this invention is obtained from the seeds of the Camellia oleifera tree, a member of the Theaceae family. It is one of China's oldest woody edible vegetable oils. China has the most widespread distribution of Camellia plants in the world and is the world's largest tea oil production base. Small quantities are found only in Southeast Asia and Japan. Camellia seed oil is highly nutritious, containing fatty acids (93% unsaturated fatty acids, including 82% oleic acid and 11% linoleic acid), camellia glycosides, tea polyphenols, saponins, tannins, squalene, wax esters, and phospholipids.

[0014] The prickle oil disclosed herein is an edible oil processed from prickle fruit and widely used in public health, skincare, and beauty products. Existing scientific research and analysis indicate that the ratio of monounsaturated, polyunsaturated, and saturated fatty acids in prickle oil is 1:1:0.8, close to the human fatty acid structure (1:1:1). The oil is also rich in the ceramide precursor phytosphingosine, vitamins E and A, and its composition closely resembles human lipids.

[0015] In some embodiments, in step S1, the mass ratio of the deep-sea fish oil, camellia seed oil, and utilitarian fruit oil is 1-10:1-10:1-10.

[0016] In one embodiment, in step S1, the mass ratio of the deep-sea fish oil, camellia seed oil, and utilitarian fruit oil is 2:3:5.

[0017] In some embodiments, in step S2, the concentration of the Candida lipolytica seed solution is 1×10 5 -10 8 CFU / mL, the inoculation amount of the Candida lipolytica seed solution is 5-10% (v / v).

[0018] In some embodiments, in step S2, the liquid culture medium is YM liquid culture medium. The YM liquid culture medium of the present invention can be a commercially available product or homemade. The YM liquid culture medium comprises 3.0 g / L yeast extract powder, 3.0 g / L malt extract powder, 10.0 g / L glucose, and 5.0 g / L casein peptone. The preparation method is to dissolve 21.0 g of the dry powder culture medium in 1000 mL of distilled water by heating, divide the mixture into small test tubes, sterilize the mixture by autoclaving at 121°C for 15 minutes, and dry the mixture for later use.

[0019] In some embodiments, in step S2, the induction culture refers to adding the oil mixture prepared in S1 to the liquid culture medium and aerobically culturing at 26-28°C for 24-48 hours; the amount of the oil mixture added is 1-2% (v / v).

[0020] In some embodiments, in step S3, the amount of the oil mixture added is 20-40% (v / v) of the volume of the culture medium.

[0021] In some embodiments, in step S3, the fermentation condition is aerobic culture at 26-28° C. for 48-72 hours.

[0022] In some embodiments, in step S4, the lower aqueous fermentation broth is discarded through a separatory funnel and the upper oil phase is collected; the centrifugal filtration treatment is first performed by centrifugation at 10,000-20,000 rpm using a tubular centrifuge, and then by 0.22 μm microfiltration to obtain a clear and sterile filtrate, which is the biomimetic skin lipid.

[0023] Another object of the present invention is to apply the above-mentioned biomimetic skin lipids in skin care products.

[0024] The biomimetic skin lipids produced by the present invention can be added as an active ingredient to cosmetic bases to achieve benefits such as maintaining skin barrier function, moisturizing, anti-inflammatory, and regulating microecology. The cosmetic bases can be essence oils, creams, lotions, facial masks, conditioners, etc.

[0025] An embodiment of the present invention also provides a water-in-oil barrier cream, comprising the following components in percentage by weight: 5% isododecane, 3% isononyl isononanoate, 3-5% of the above-mentioned biomimetic skin lipids, 0.5% polyglyceryl-6 polyricinoleate, 0.5% polyglyceryl-6 pentaoleate, 0.5% p-hydroxyacetophenone, 0.6% sodium chloride, 0.5% 1,2-hexanediol, 5% butylene glycol, 0.05% xanthan gum, 0.5% betaine, and the balance water.

[0026] The beneficial effects brought by the technical scheme provided by the embodiment of the present application are that the composite vegetable oil composed of deep sea fish oil, camellia seed oil and Chinese pricklyash fruit oil is rich in skin required lipids such as triacylglycerol, phytosphingosine, squalene, cholesteryl ester, cholesterol, wax ester and phospholipid; the composite vegetable oil is fermented by using Candida lipolytica, and a kind of biomimetic skin lipid is obtained after fermentation, and the content of effective components and the use feeling are obviously improved, which can be added as an active ingredient into a cosmetic matrix to play the multi-dimensional effects of maintaining skin barrier function, moisturizing, anti-inflammatory and regulating microecology. DETAILED DESCRIPTION

[0027] The present application discloses a kind of biomimetic skin lipid and preparation method thereof.The person skilled in the art can improve process parameters appropriately according to the content herein.The particularly need to point out is, all similar replacements and changes are obvious to the person skilled in the art, and they are all considered to be included in the present application.The method and application of the present application have been described by preferred embodiments, and the relevant personnel can obviously modify or appropriately change and combine the method and application described herein without departing from the content, spirit and scope of the present application, to implement and apply the present application technology.

[0028] The biomimetic skin lipid provided by the present application and the raw materials and reagents used in the preparation method thereof can be purchased from the market.

[0029] The present application is further described below in combination with embodiments:

[0030] Embodiment 1

[0031] A kind of biomimetic skin lipid, which is prepared by the following preparation method:

[0032] S1, deep sea fish oil, camellia seed oil, Chinese pricklyash fruit oil are mixed according to the mass ratio 1:10:1 to obtain an oil mixture;

[0033] S2, the seed bacteria liquid of Candida lipolytica with a concentration of 1×10 8 CFU / mL is inoculated into YM liquid culture medium, and the bacteria liquid is added in an amount of 5% (v / v) of the culture medium; the oil mixture prepared in S1 is added to the liquid culture medium, and the oil mixture is added in an amount of 2% (v / v) of the culture medium; aerobic induction culture is carried out at 26°C for 48 hours to obtain a culture solution;

[0034] S3, the oil mixture prepared in S1 is added to the culture solution, and the oil mixture is added in an amount of 20% (v / v) of the volume of the culture solution; aerobic fermentation culture is carried out at 28°C for 48 hours to obtain a fermentation solution;

[0035] S4, discard the lower aqueous fermentation liquid through a separatory funnel and collect the upper oil phase; centrifuge at 20,000 rpm using a tubular centrifuge, and then filter through a 0.22 μm microporous filter to obtain a clear and sterile filtrate, which is the biomimetic skin lipid.

[0036] Example 2

[0037] A biomimetic skin lipid is obtained by the following preparation method:

[0038] S1, mixing deep-sea fish oil, camellia seed oil, and pruinosa purpurogenum oil in a mass ratio of 10:1:10 to obtain an oil mixture;

[0039] S2, the concentration is 1×10 5 A Candida lipolytica seed culture of 100 CFU / mL was inoculated into a YM liquid culture medium, wherein the culture culture was added in an amount of 10% (v / v) of the culture medium; the oil mixture prepared in S1 was added to the liquid culture medium, wherein the oil mixture was added in an amount of 1% (v / v) of the culture medium; and aerobic induction culture was performed at 28° C. for 24 hours to obtain a culture solution.

[0040] S3, adding the oil mixture prepared in S1 to the culture medium, wherein the amount of the oil mixture added is 40% (v / v) of the volume of the culture medium; aerobic fermentation is carried out at 26° C. for 72 hours to obtain a fermentation liquid;

[0041] S4, discard the lower aqueous fermentation liquid through a separatory funnel and collect the upper oil phase; centrifuge at 10,000 rpm using a tubular centrifuge, and then filter through a 0.22 μm microporous filter to obtain a clear and sterile filtrate, which is the biomimetic skin lipid.

[0042] Example 3

[0043] A biomimetic skin lipid is obtained by the following preparation method:

[0044] S1, mixing deep-sea fish oil, camellia seed oil, and pruinosa purpurogenum oil in a mass ratio of 2:3:5 to obtain an oil mixture;

[0045] S2, the concentration is 1×10 6 A Candida lipolytica seed culture of 100 CFU / mL was inoculated into YM liquid culture medium, wherein the culture culture was added in an amount of 8% (v / v) of the culture medium; the oil mixture prepared in S1 was added to the liquid culture medium, wherein the oil mixture was added in an amount of 1.5% (v / v) of the culture medium; and aerobic induction culture was carried out at 27° C. for 36 hours to obtain a culture solution.

[0046] S3, adding the oil mixture prepared in S1 into the culture solution, the oil mixture is added in an amount of 30% (v / v) of the volume of the culture solution; aerobic fermentation is carried out at 27°C for 60 hours to obtain a fermentation solution;

[0047] S4, discarding the lower aqueous phase fermentation solution through a separatory funnel, and collecting the upper oil phase layer; centrifugation is carried out at 15000 rpm using a tubular centrifuge, and then 0.22 μm microporous filtration is carried out to obtain a clear sterile filtrate, which is the biomimetic skin lipid.

[0048] Comparative Example 1

[0049] A biomimetic skin lipid is prepared by the following preparation method:

[0050] S1, mixing deep sea fish oil, camellia seed oil and Chinese pricklyash fruit oil according to a mass ratio of 2:3:5 to obtain an oil mixture.

[0051] Test 1 Sensory evaluation

[0052] Twelve trained inspectors are convened to perform sensory evaluation on the biomimetic skin lipids prepared in Examples 1-3 and Comparative Example 1. The specific test method is as follows: 1 mL of sample is dropped on the inner side of the arm, and a circle rubbing test is performed, the spreading property (the ease of movement of the sample on the skin), the oiliness (the degree of oiliness of the product to the skin) and the absorption (the ease of absorption by the product through the number of rubbing circles) are felt, and then each sensory index is scored according to 1-9, and the arithmetic mean of each sample is calculated, as shown in Table 1.

[0053] Table 1 Sensory evaluation record

[0054]

[0055] As can be seen from Table 1, the spreading property and the absorption of the biomimetic skin lipids prepared in Examples 1-3 after fermentation are improved, and the oiliness is reduced compared with the conventional oil mixture.

[0056] Comparative Example 2

[0057] A biomimetic skin lipid is prepared by the following preparation method:

[0058] S1, mixing deep sea fish oil to obtain an oil mixture;

[0059] S2, adding 1×10 6A Candida lipolytica seed culture of 100 CFU / mL was inoculated into YM liquid culture medium, wherein the culture culture was added in an amount of 8% (v / v) of the culture medium; the oil mixture prepared in S1 was added to the liquid culture medium, wherein the oil mixture was added in an amount of 1.5% (v / v) of the culture medium; and aerobic induction culture was carried out at 27° C. for 36 hours to obtain a culture solution.

[0060] S3, adding the oil mixture prepared in S1 to the culture medium, wherein the amount of the oil mixture added is 30% (v / v) of the volume of the culture medium; aerobic fermentation is carried out at 27° C. for 60 hours to obtain a fermentation liquid;

[0061] S4, discard the lower aqueous fermentation liquid through a separatory funnel and collect the upper oil phase; centrifuge at 15,000 rpm using a tubular centrifuge, and then filter through a 0.22 μm microporous filter to obtain a clear and sterile filtrate, which is the biomimetic skin lipid.

[0062] Comparative Example 3

[0063] A biomimetic skin lipid is obtained by the following preparation method:

[0064] S1, mixing camellia seed oil to obtain an oil mixture;

[0065] S2, the concentration is 1×10 6 A Candida lipolytica seed culture of 100 CFU / mL was inoculated into YM liquid culture medium, wherein the culture culture was added in an amount of 8% (v / v) of the culture medium; the oil mixture prepared in S1 was added to the liquid culture medium, wherein the oil mixture was added in an amount of 1.5% (v / v) of the culture medium; and aerobic induction culture was carried out at 27° C. for 36 hours to obtain a culture solution.

[0066] S3, adding the oil mixture prepared in S1 to the culture medium, wherein the amount of the oil mixture added is 30% (v / v) of the volume of the culture medium; aerobic fermentation is carried out at 27° C. for 60 hours to obtain a fermentation liquid;

[0067] S4, discard the lower aqueous fermentation liquid through a separatory funnel and collect the upper oil phase; centrifuge at 15,000 rpm using a tubular centrifuge, and then filter through a 0.22 μm microporous filter to obtain a clear and sterile filtrate, which is the biomimetic skin lipid.

[0068] Comparative Example 4

[0069] A biomimetic skin lipid is obtained by the following preparation method:

[0070] S1, mixing utilitarian fruit oil to obtain an oil mixture;

[0071] S2, the concentration is 1×10 6A Candida lipolytica seed culture of 100 CFU / mL was inoculated into YM liquid culture medium, wherein the culture culture was added in an amount of 8% (v / v) of the culture medium; the oil mixture prepared in S1 was added to the liquid culture medium, wherein the oil mixture was added in an amount of 1.5% (v / v) of the culture medium; and aerobic induction culture was carried out at 27° C. for 36 hours to obtain a culture solution.

[0072] S3, adding the oil mixture prepared in S1 to the culture medium, wherein the amount of the oil mixture added is 30% (v / v) of the volume of the culture medium; aerobic fermentation is carried out at 27° C. for 60 hours to obtain a fermentation liquid;

[0073] S4, discard the lower aqueous fermentation liquid through a separatory funnel and collect the upper oil phase; centrifuge at 15,000 rpm using a tubular centrifuge, and then filter through a 0.22 μm microporous filter to obtain a clear and sterile filtrate, which is the biomimetic skin lipid.

[0074] Comparative Example 5

[0075] A biomimetic skin lipid is obtained by the following preparation method:

[0076] S1, mixing deep-sea fish oil, camellia seed oil, and pruinosa purpurogenum oil in a mass ratio of 2:3:5 to obtain an oil mixture;

[0077] S2, the concentration is 1×10 6 CFU / mL of Candida lipolytica seed culture was inoculated into YM liquid culture medium, wherein the amount of the culture culture added was 8% (v / v) of the culture medium; aerobic induction culture was performed at 27°C for 36 hours to obtain a culture medium;

[0078] S3, adding the oil mixture prepared in S1 to the culture medium, wherein the amount of the oil mixture added is 30% (v / v) of the volume of the culture medium; aerobic fermentation is carried out at 27° C. for 60 hours to obtain a fermentation liquid;

[0079] S4, discard the lower aqueous fermentation liquid through a separatory funnel and collect the upper oil phase; centrifuge at 15,000 rpm using a tubular centrifuge, and then filter through a 0.22 μm microporous filter to obtain a clear and sterile filtrate, which is the biomimetic skin lipid.

[0080] Test of 3DPPH free radical scavenging ability

[0081] As a stable free radical, DPPH can exist stably in organic solvents. Its alcohol solution is purple and needs to be stored at low temperature and away from light. It has a single electron, so it can accept an electron or hydrogen ion and has a maximum absorption at a wavelength of 517nm. In the presence of a free radical scavenger, the single electron of DPPH is captured, causing its color to become lighter. The absorbance at the wavelength of maximum light absorption decreases, and the degree of decrease is linear. The decrease in absorbance level indicates an increase in antioxidant activity, thereby evaluating the antioxidant capacity of the test sample. This antioxidant capacity is expressed as an inhibition rate. The greater the inhibition rate, the stronger the antioxidant capacity. The biomimetic skin lipids prepared in Examples 1-3 and Comparative Examples 1-5 were respectively prepared into 20 mg / mL dimethyl sulfoxide solutions, and then the free radical capacity test was performed according to the method described in the document "Han Jiejun, Gong Tiangui, Wang Bin, et al. Study on process optimization and efficacy activity of peony seed fermentation oil [J]. Modern Chemical Industry, 2024, 44(S01): 235-239." The specific test results are shown in Table 2.

[0082] Table 2 DPPH free radical scavenging ability

[0083] Test Group DPPH clearance rate / % Example 1 62.8 Example 2 69.4 Example 3 72.5 Comparative Example 1 30.9 Comparative Example 2 9.4 Comparative Example 3 16.7 Comparative Example 4 21.2 Comparative Example 5 54.9

[0084] As can be seen from Table 2, the DPPH free radical scavenging rate of the biomimetic skin lipids prepared in Examples 1-3 is 62.8-72.5%, which is more than 30% higher than that of Comparative Example 1 of conventional oil, indicating that a better antioxidant effect is achieved through fermentation; the free radical scavenging rates of Comparative Examples 2-4 using deep-sea fish oil, camellia seed oil, and pruinosa fruit oil alone are between 36.7-41.2%, which is lower than 72.5% of Example 3, indicating that the combined use of deep-sea fish oil, camellia seed oil, and pruinosa fruit oil has a better effect. The free radical scavenging rate of Comparative Example 5 is 54.9%, which is lower than 72.5% of Example 3, indicating that the induction culture in step S2 contributes to improving the DPPH free radical scavenging rate of the biomimetic skin lipids.

[0085] Example 4

[0086] A water-in-oil barrier cream comprises the following components in percentage by weight: 5% isododecane, 3% isononyl isononanoate, 5% of the above-mentioned biomimetic skin lipid, 0.5% polyglyceryl-6 polyricinoleate, 0.5% polyglyceryl-6 pentaoleate, 0.5% p-hydroxyacetophenone, 0.6% sodium chloride, 0.5% 1,2-hexanediol, 5% butylene glycol, 0.05% xanthan gum, 0.5% betaine, and the balance water.

[0087] Example 5

[0088] A water-in-oil barrier cream comprises the following components in percentage by weight: 5% isododecane, 3% isononyl isononanoate, 3% of the above-mentioned biomimetic skin lipid, 0.5% polyglyceryl-6 polyricinoleate, 0.5% polyglyceryl-6 pentaoleate, 0.5% p-hydroxyacetophenone, 0.6% sodium chloride, 0.5% 1,2-hexanediol, 5% butylene glycol, 0.05% xanthan gum, 0.5% betaine, and the balance water.

[0089] Comparative Example 6

[0090] A water-in-oil barrier cream comprises the following components in percentage by weight: 5% isododecane, 3% isononyl isononanoate, 3% of the above-mentioned biomimetic skin lipid, 1% polyglyceryl-6 polyricinoleate, 0.5% p-hydroxyacetophenone, 0.6% sodium chloride, 0.5% 1,2-hexanediol, 5% butylene glycol, 0.05% xanthan gum, 0.5% betaine, and the balance water.

[0091] Comparative Example 7

[0092] A water-in-oil barrier cream comprises the following components in percentage by weight: 5% isododecane, 3% isononyl isononanoate, 3% of the above-mentioned biomimetic skin lipid, 1% polyglyceryl-6 pentaoleate, 0.5% p-hydroxyacetophenone, 0.6% sodium chloride, 0.5% 1,2-hexanediol, 5% butylene glycol, 0.05% xanthan gum, 0.5% betaine, and the balance water.

[0093] Test 3 Stability Test

[0094] Stability tests were conducted on the water-in-oil barrier creams prepared in Examples 4-5 and Comparative Examples 5-6. The products' heat and cold stability indicators were evaluated according to the industry standard "QB / T 1857-2004 Moisturizing Creams." The acceptable heat resistance indicator was a 24-hour oil permeation rate of ≤3% after being held at (40±1)°C and returning to room temperature. The acceptable cold resistance indicator was a 24-hour exposure to -5°C to -10°C and no significant difference in properties after returning to room temperature. See Table 3 for detailed test results.

[0095] Table 3 Stability test records

[0096] Test Group Heat resistant Cold-resistant Example 4 pass pass Example 5 pass pass Comparative Example 6 pass Cold resistance, demulsification and stratification, failed Comparative Example 7 pass Cold resistance, demulsification and stratification, failed

[0097] As shown in Table 3, the water-in-oil barrier cream prepared in Examples 4-5 passed the heat resistance and cold resistance tests specified in the industry standard, while the water-in-oil barrier cream prepared in Comparative Examples 6-7 was demulsified and separated when subjected to the cold resistance test. When subjected to the cold resistance test, the water phase of the water-in-oil system may freeze at -5℃ to -10℃, and if the interface film formed by the emulsifier is not strong enough, the frozen water phase may break through the interface film and enter the external phase, and after returning to room temperature, the phenomenon of demulsification and separation may occur. It is estimated that the cold resistance of the water-in-oil system in Examples 4-5 is due to the stronger strength and elasticity of the interface film formed by the combination of polyglyceryl-6 polyricinoleate and polyglyceryl-6 pentaoleate.

[0098] Test 5: Volunteer test

[0099] The skin barrier is the natural protective layer of the skin, which is composed of the stratum corneum and the sebum film. Once damaged, the skin may exhibit abnormal reactions due to the decreased water retention capacity and weakened defense. The main manifestations of damaged skin barrier include dryness and desquamation, redness and sensitivity, easy irritation, repeated inflammation or itching, etc. Volunteers with damaged skin barrier were recruited from the society to test the water-in-oil barrier cream prepared in Example 5. Twenty volunteers with dryness and desquamation, redness and sensitivity, and easy irritation on the face were recruited, regardless of gender, and aged 35-55 years. After washing the face, the volunteers applied an appropriate amount of the sample to the face, massaged and absorbed, once in the morning and once in the evening, and continuously used for 4 weeks. After 4 weeks, it was found that the skin barrier damage of 13 volunteers was greatly improved, and the significant efficiency reached 65%; the skin barrier damage of 5 volunteers was improved to a certain extent, and the total efficiency reached 90%. In addition, all the volunteers indicated that no irritation and allergy occurred during the use of the product, and the product had a light and silky texture without greasiness. It can be seen that the biomimetic skin lipid prepared in the present application achieves good skin barrier repair, moisturizing and anti-inflammatory effects when applied in cosmetic formulations.

[0100] The above only describes the preferred embodiments of the present application, and it should be noted that those skilled in the art can make several improvements and refinements without departing from the principles of the present application, and these improvements and refinements should also be considered within the protection scope of the present application.

Claims

1. A bionic skin lipid, characterized in that: It is obtained by the following preparation method: S1, mixing deep-sea fish oil, camellia seed oil, and pruinosa purpurogenum oil to obtain an oil mixture; S2, inoculating the Candida lipolytica seed culture into a liquid culture medium for induction culture to obtain a culture solution; S3, adding the oil mixture to the culture solution and fermenting to obtain a fermentation solution; S4, collecting the oil phase layer on the upper layer of the fermentation liquid, centrifuging and filtering, and obtaining a clear and sterile filtrate, which is the biomimetic skin lipid.

2. The biomimetic skin lipid according to claim 1, characterized in that In step S1, the mass ratio of the deep-sea fish oil, camellia seed oil, and utilitarian fruit oil is 1-10:1-10:1-10.

3. The biomimetic skin lipid according to claim 2, characterized in that In step S1, the mass ratio of the deep-sea fish oil, camellia seed oil, and utilitarian fruit oil is 2:3:

5.

4. The biomimetic skin lipid according to claim 1, characterized in that In step S2, the concentration of the Candida lipolytica seed solution is 1×10 5 -10 8 CFU / mL, the inoculation amount of the Candida lipolytica seed solution is 5-10% (v / v).

5. The biomimetic skin lipid according to claim 1, characterized in that In step S2, the liquid culture medium is YM liquid culture medium.

6. The biomimetic skin lipid according to claim 1, characterized in that In step S2, the induction culture refers to adding the oil mixture prepared in S1 to the liquid culture medium and aerobically culturing at 26-28°C for 24-48 hours; the amount of the oil mixture added is 1-2% (v / v).

7. The biomimetic skin lipid according to claim 1, characterized in that In step S3, the amount of the oil mixture added is 20-40% (v / v) of the volume of the culture solution; and the fermentation conditions are aerobic culture at 26-28° C. for 48-72 hours.

8. The biomimetic skin lipid according to claim 1, characterized in that In step S4, the lower aqueous fermentation broth is discarded through a separatory funnel and the upper oil phase is collected; the centrifugal filtration treatment is first performed by centrifuging at 10,000-20,000 rpm in a tubular centrifuge, and then by 0.22 μm microfiltration to obtain a clear and sterile filtrate, which is the biomimetic skin lipid.

9. Use of the biomimetic skin lipid according to any one of claims 1 to 8 in the preparation of cosmetics.

10. A water-in-oil barrier cream, characterized in that: The invention comprises the following components in percentage by weight: 5% of isododecane, 3% of isononyl isononanoate, 3-5% of the biomimetic skin lipid according to any one of claims 1 to 8, 0.5% of polyglycerol-6 polyricinoleate, 0.5% of polyglycerol-6 pentaoleate, 0.5% of p-hydroxyacetophenone, 0.6% of sodium chloride, 0.5% of 1,2-hexanediol, 5% of butylene glycol, 0.05% of xanthan gum, 0.5% of betaine, and the balance of water.

Images