Inorganic oxide-based nano-enzyme, preparation method thereof and method for adjusting oxidation resistance of inorganic oxide-based nano-enzyme

Abstract

The invention provides an inorganic oxide-based nano-enzyme, a preparation method thereof and a method for adjusting the oxidation resistance of the inorganic oxide-based nano-enzyme, and belongs to the technical field of nano-materials. Nano-selenium is adsorbed to the surface of inorganic oxide nano-enzyme, surface stress is introduced to the surface of the inorganic oxide nano-enzyme, local lattice distortion of inorganic oxide is induced, and high-density and uniformly-distributed surface oxygen vacancies are directionally generated; by limiting the molar ratio of the nano-selenium to the inorganic oxide nano-enzyme, the oxygen vacancy can be kept at a relatively high level; when a magnetic field is applied to the inorganic oxide nano-enzyme, high-density oxygen vacancies migrate in the magnetic field, and the magnetic field forms Lorentz force on the charged oxygen vacancies, so that a directional driving effect is generated on a migration path of the oxygen vacancies, the oxygen vacancy regeneration rate and ROS removal kinetics are remarkably accelerated, and the oxygen vacancy regeneration rate and ROS removal kinetics are remarkably improved. The oxidation resistance of the nano-enzyme can be regulated and controlled in real time according to needs by regulating and controlling the magnetic field intensity.

Description

Technical Field

[0001] The present invention belongs to the technical field of nanomaterials, and specifically relates to an inorganic oxide-based nanozyme, a preparation method thereof, and a method for regulating antioxidant capacity. Background Art

[0002] Nanozymes are a class of nanomaterials with enzymatic catalytic properties. Their unique size, surface, and quantum effects have attracted considerable attention in fields such as biomedicine and environmental management. Inorganic oxide nanozymes have become a key branch of nanozyme research due to their excellent chemical stability, catalytic performance, and ease of synthesis and modification.

[0003] Inorganic oxide nanozymes can efficiently scavenge reactive oxygen species (ROS) or degrade organic pollutants by mimicking the catalytic mechanism of natural oxidases, showing potential as a biomedical alternative to natural enzymes. However, the slow regeneration rate of oxygen vacancies in the dynamic reactions of existing inorganic oxide nanozymes limits their antioxidant capacity, severely restricting their practical applications.

[0004] In addition, due to the fixed catalytic activity of inorganic oxide nanozymes, they cannot cope with dynamically changing oxidative stress levels after implantation. From the perspective of the tissue repair cycle, after tissue damage, it is necessary to maintain a high oxidative stress level for about 24 hours, and then reduce the oxidative stress level within a week to promote tissue repair. In the later stage of tissue repair, it is necessary to maintain a certain oxidative stress level to induce cells to secrete ECM, etc., to accelerate tissue repair. Therefore, if the dosage of inorganic oxide nanozymes is too much, it is not conducive to reducing the oxidative stress level 24 hours after damage and during the tissue repair period; if the dosage is too little, it cannot meet the high stress level requirements within 24 hours of damage. Therefore, providing an inorganic oxide nanozyme with adjustable antioxidant capacity so that the antioxidant capacity of inorganic oxide nanozymes can be regulated on demand has important application value. Summary of the Invention

[0005] The present invention aims to provide an inorganic oxide-based nanozyme, a preparation method thereof, and a method for regulating antioxidant capacity. The inorganic oxide-based nanozyme provided by the present invention can regulate antioxidant capacity according to demand to meet the needs of different oxidative stress levels.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The present invention provides an inorganic oxide-based nanozyme, comprising an inorganic oxide nanozyme and nano-selenium adsorbed on the surface of the inorganic oxide nanozyme; the lattice of the inorganic oxide nanozyme contains oxygen vacancies; the molar ratio of the nano-selenium to the inorganic oxide nanozyme is 1:(5-20).

[0008] Preferably, the molar ratio of the nano-selenium to the inorganic oxide nanozyme is 1:(10-15).

[0009] Preferably, the inorganic oxide nanozyme comprises nano-cerium oxide, nano-manganese oxide or nano-copper oxide.

[0010] The present invention also provides a method for preparing the inorganic oxide-based nanozyme described in the above technical solution, comprising: mixing a nano-selenium suspension with an inorganic oxide nanozyme for in-situ adsorption to obtain an inorganic oxide-based nanozyme.

[0011] Preferably, the in-situ adsorption time is 10 to 20 hours.

[0012] Preferably, the concentration of nano-selenium in the nano-selenium suspension is 30-250 μg / mL.

[0013] Preferably, the nano-selenium suspension is prepared by chemical reduction, biosynthesis or physical preparation.

[0014] The present invention also provides a method for regulating the antioxidant capacity of an inorganic oxide-based nanozyme, wherein a magnetic field is applied to the inorganic oxide-based nanozyme, and the inorganic oxide-based nanozyme is the inorganic oxide-based nanozyme described in the above technical solution.

[0015] Preferably, the intensity of the magnetic field is no higher than 2000 mT.

[0016] Preferably, the magnetic field is a static magnetic field or an alternating magnetic field.

[0017] The present invention provides an inorganic oxide-based nanozyme, comprising an inorganic oxide nanozyme and nano-selenium adsorbed on the surface of the inorganic oxide nanozyme; the lattice of the inorganic oxide nanozyme contains oxygen vacancies; the molar ratio of the nano-selenium to the inorganic oxide nanozyme is 1:(5-20). The present invention adsorbs nano-selenium on the surface of the inorganic oxide nanozyme, introduces surface stress on the surface of the inorganic oxide nanozyme, induces local lattice distortion of the inorganic oxide, and directionally generates high-density, uniformly distributed surface oxygen vacancies; the increase in oxygen vacancies can promote the transfer and distribution of electrons, making it easier for it to react with the substrate and enhancing the antioxidant performance; by limiting the molar ratio of nano-selenium to inorganic oxide nanozyme, the oxygen vacancies can be maintained at a high level; when a magnetic field is applied to the inorganic oxide nanozyme, the high-density oxygen vacancies migrate in the magnetic field, and the magnetic field forms a Lorentz force on the charged oxygen vacancies, thereby generating a directional driving effect on the migration path of the oxygen vacancies, significantly accelerating the oxygen vacancy regeneration rate and ROS scavenging kinetics, the greater the magnetic field intensity, the greater the Lorentz force on the oxygen vacancies, the faster the migration speed, the more conducive to the generation of new oxygen vacancies, and thus the greater the effect of improving the antioxidant capacity, and by regulating the magnetic field intensity, the on-demand real-time regulation of the nanozyme's antioxidant capacity can be achieved. The results of the examples show that the inorganic oxide-based nanozyme provided by the present invention has a DPPH free radical scavenging rate of 64.07%, 67.40% and 71.85% when no magnetic field is applied, the magnetic field strength is 50-100mT, and the magnetic field strength is 200-300mT, respectively, which meets the usage requirements of different oxidative stress levels. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a graph showing the changes in free radical scavenging rates of the nanozymes of Examples 1 to 2 of the present invention and Comparative Example 1 in a static magnetic field;

[0019] Figure 2 This is a graph showing the changes in free radical scavenging rates of the nanozymes of Example 4 of the present invention and Comparative Example 2 in a static magnetic field;

[0020] Figure 3 This is a graph showing the changes in free radical scavenging rates of the nanozymes of Example 1 and Comparative Example 1 of the present invention in an alternating magnetic field;

[0021] Figure 4 Graph showing the free radical scavenging rate changes of the nanozymes of Examples 1 and 3 of the present invention and Comparative Example 1 in a static magnetic field;

[0022] Figure 5 This is a graph showing the changes in free radical scavenging rate of the nanozyme in Example 1 of the present invention in magnetic fields of different intensities. DETAILED DESCRIPTION

[0023] All raw materials of the present invention are not particularly limited in their sources and can be purchased from the market or prepared according to conventional methods well known to those skilled in the art.

[0024] The purity of all raw materials in the present application is not particularly limited, and the present application preferably uses analytically pure raw materials.

[0025] The present application provides an inorganic oxide-based nanoscale enzyme, comprising an inorganic oxide nanoscale enzyme and nano-selenium adsorbed on the surface of the inorganic oxide nanoscale enzyme; the crystal lattice of the inorganic oxide nanoscale enzyme contains oxygen vacancies; and the molar ratio of the nano-selenium to the inorganic oxide nanoscale enzyme is 1:(5-20).

[0026] The inorganic oxide-based nanoscale enzyme provided by the present application comprises an inorganic oxide nanoscale enzyme, and the crystal lattice of the inorganic oxide nanoscale enzyme contains oxygen vacancies. The inorganic oxide nanoscale enzyme containing oxygen vacancies can promote the transfer and distribution of electrons through oxygen vacancies, and has antioxidant capacity by undergoing redox reactions with oxygen free radicals of substrates.

[0027] In the present application, the inorganic oxide nanoscale enzyme preferably comprises nano-cerium oxide, nano-manganese oxide or nano-copper oxide. The above nano-oxides have more oxygen vacancies, which are beneficial to the adjustment of antioxidant capacity by a magnetic field.

[0028] The inorganic oxide-based nanoscale enzyme provided by the present application further comprises nano-selenium adsorbed on the surface of the inorganic oxide nanoscale enzyme.

[0029] In the present application, the molar ratio of the nano-selenium to the inorganic oxide nanoscale enzyme is 1:(5-20), preferably 1:(10-15); as an embodiment of the present application, the molar ratio of the nano-selenium to the inorganic oxide nanoscale enzyme can be 1:5, 1:7, 1:9, 1:10, 1:12, 1:14, 1:15, 1:16, 1:18 or 1:20. The nano-selenium is adsorbed on the surface of the inorganic oxide nanoscale enzyme, which can introduce surface stress on the surface of the inorganic oxide, induce local lattice distortion of the inorganic oxide, and directionally generate high-density and uniformly distributed surface oxygen vacancies; the molar ratio of the nano-selenium to the inorganic oxide nanoscale enzyme in the above range can keep the oxygen vacancies at a high level, which is beneficial to the regulation of the antioxidant capacity of the inorganic oxide-based nanoscale enzyme by a magnetic field.

[0030] The present invention adsorbs nano-selenium on the surface of the inorganic oxide nanozyme, introduces surface stress on the surface of the inorganic oxide nanozyme, induces local lattice distortion of the inorganic oxide, and directionally generates high-density, uniformly distributed surface oxygen vacancies; the increase in oxygen vacancies can promote the transfer and distribution of electrons, making it easier for it to react with the substrate and enhancing the antioxidant performance; by limiting the molar ratio of nano-selenium to inorganic oxide nanozyme, the oxygen vacancies can be maintained at a high level; when a magnetic field is applied to the inorganic oxide nanozyme, the high-density oxygen vacancies migrate in the magnetic field, and the magnetic field forms a Lorentz force on the charged oxygen vacancies, thereby generating a directional driving effect on the migration path of the oxygen vacancies, significantly accelerating the oxygen vacancy regeneration rate and ROS scavenging kinetics, the greater the magnetic field intensity, the greater the Lorentz force on the oxygen vacancies, the faster the migration speed, the more conducive to the generation of new oxygen vacancies, and thus the greater the effect of improving the antioxidant capacity, and by regulating the magnetic field intensity, the on-demand real-time regulation of the nanozyme's antioxidant capacity can be achieved.

[0031] The present invention also provides a method for preparing the inorganic oxide-based nanozyme described in the above technical solution, comprising: mixing a nano-selenium suspension with an inorganic oxide nanozyme for in-situ adsorption to obtain an inorganic oxide-based nanozyme.

[0032] In the present invention, the concentration of the nano-selenium suspension is preferably 30 to 250 μg / mL, more preferably 100 to 150 μg / mL. As one embodiment of the present invention, the concentration of the nano-selenium suspension can be 39.5 μg / mL, 79 μg / mL, 158 μg / mL, 200 μg / mL, or 250 μg / mL. The concentration of the nano-selenium suspension within the above range is conducive to the adsorption of nano-selenium on the surface of the inorganic oxide nanozyme.

[0033] In the present invention, the nano-selenium suspension is preferably prepared by chemical reduction, biosynthesis, or physical preparation, more preferably chemical reduction. As one embodiment of the present invention, the nano-selenium suspension can be obtained by reduction reaction of sodium selenite (Na2SeO3) and ascorbic acid in deionized water. The molar ratio of Na2SeO3 to ascorbic acid can be 1:(3-4), and the reduction reaction can be carried out under stirring. The above method is conducive to suspending the nano-selenium in the solution.

[0034] In the present invention, the in situ adsorption time is preferably 10 to 20 hours, more preferably 12 to 16 hours. As one embodiment of the present invention, the in situ adsorption time can be 11 hours, 12 hours, 13 hours, 14 hours, 15 hours, 17 hours, or 18 hours. In situ adsorption time within the above range allows for sufficient adsorption of nano-selenium and inorganic oxide nanozymes, which is beneficial for improving the adsorption effect.

[0035] As one embodiment of the present invention, the in situ adsorption can be stirred to promote the adsorption of nano-selenium onto the surface of the inorganic oxide nanozyme. The present invention has no particular requirements for the stirring rate, as long as it does not affect the in situ adsorption process. After adsorption is completed, the inorganic oxide nanozyme adsorbed with nano-selenium is deposited at the bottom of the solution in the form of a precipitate.

[0036] As an embodiment of the present invention, the mixed system obtained by in situ adsorption can be separated into solid and liquid by centrifugation, and the obtained solid is the inorganic oxide-based nanozyme; the present invention has no special limitation on the parameters of the centrifugation, as long as the precipitate can be separated.

[0037] As an embodiment of the present invention, the inorganic oxide-based nanozyme can be cleaned with deionized water and anhydrous ethanol; the present invention does not particularly limit the cleaning method, and conventional cleaning methods in the art can be used.

[0038] As an embodiment of the present invention, the inorganic oxide-based nanozyme can be dried after washing; the present invention has no special requirements for the drying method, as long as the solvent in the solid can be completely removed.

[0039] As an embodiment of the present invention, the inorganic oxide-based nanozyme can be ground after drying; the present invention has no special requirements on the parameters of the grinding, as long as the solid can be ground into powder.

[0040] The preparation method provided by the present invention is simple to operate, does not require special equipment, has broad process requirements, and is conducive to improving the performance stability of the product.

[0041] The present invention also provides a method for regulating the antioxidant capacity of an inorganic oxide-based nanozyme, wherein a magnetic field is applied to the inorganic oxide-based nanozyme, and the inorganic oxide-based nanozyme is the inorganic oxide-based nanozyme described in the above technical solution.

[0042] In the present invention, the intensity of the magnetic field is preferably no greater than 2000 mT, more preferably 200-1000 mT. As one embodiment of the present invention, the intensity of the magnetic field can be 50 mT, 80 mT, 200 mT, 500 mT, 800 mT, 1000 mT, 1200 mT, 1600 mT, or 1800 mT. A magnetic field intensity within the above range can enhance the antioxidant capacity of inorganic oxide-based nanozymes without causing damage to the human body, thus facilitating the practical application of nanozymes.

[0043] In the present invention, the magnetic field is preferably a static magnetic field or an alternating magnetic field; as an embodiment of the present invention, the frequency of the alternating magnetic field can be 1 to 250 KHz.

[0044] As an embodiment of the present invention, when the magnetic field is a static magnetic field, the intensity of the magnetic field can be a range value within a certain interval, specifically 50-100mT, 200-300mT, 500-600mT, 800-900mT or 1500-1600mT.

[0045] The present invention regulates the antioxidant capacity of inorganic oxide-based nanozymes by applying a magnetic field, thereby realizing real-time regulation of the antioxidant capacity of nanozymes through in vitro intervention. Moreover, the human body has a certain tolerance to magnetic fields, and regulation can be performed without any adverse effects on the human body, which is conducive to the practical application of nanozymes.

[0046] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions of the present invention. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0047] Example 1

[0048] An inorganic oxide-based nanozyme is composed of cerium oxide nanozyme and nano-selenium adsorbed on its surface, with a molar ratio of nano-selenium to cerium oxide nanozyme of 1:10, and is denoted as 1Se-CeO2.

[0049] The preparation method is as follows:

[0050] Dissolve 0.0173 g of Na2SeO3 and 0.06 g of ascorbic acid in 100 mL of deionized water and stir to generate a nano-selenium suspension;

[0051] 0.17 g of nano-cerium oxide was added to the nano-selenium suspension, stirred for 12 hours, and then centrifuged. The precipitate was washed with deionized water and anhydrous ethanol, dried, and ground into powder, which is the inorganic oxide-based nanozyme.

[0052] Example 2

[0053] An inorganic oxide-based nanozyme, having the same composition as in Example 1, except that the molar ratio of nanoselenium to cerium oxide nanozyme is 1:5, denoted as 2Se-CeO2.

[0054] Example 3

[0055] An inorganic oxide-based nanozyme, having the same composition as in Example 1, except that the molar ratio of nanoselenium to cerium oxide nanozyme is 1:20, denoted as 0.5Se-CeO2.

[0056] Example 4

[0057] An inorganic oxide-based nanosensor, which is a cerium oxide nanosensor, is denoted as CeO2.

[0058] Comparative Example 1

[0059] An inorganic oxide-based nanosensor, which is a cerium oxide nanosensor, is denoted as CeO2.

[0060] Comparative Example 2

[0061] An inorganic oxide-based nanosensor, which is a cerium oxide nanosensor, is denoted as CeO2.

[0062] Test Example 1

[0063] 0.1 mg of the inorganic oxide-based nanosensor was dispersed in 1 mL of anhydrous ethanol to form a nanosensor solution; the nanosensor solution was mixed with an equal volume of a DPPH solution of 0.2 mmol / L to obtain a test solution; an equal volume of anhydrous ethanol was used to replace the mixture of the nanosensor solution and the DPPH solution as a blank control solution; the sample solution was obtained by oscillation in a magnetic field of a certain intensity (25°C) in the dark for 30 min; the absorbance of the solution at 517 nm was determined by using an enzyme-labeled instrument after high-speed centrifugation (15000 rpm) of the sample solution for 5 min; and the DPPH free radical scavenging rate was calculated according to the following formula:

[0064]

[0065] In the formula, A0represents the absorbance of the blank control solution;

[0066] A1represents the absorbance of the sample solution;

[0067] Each test was performed in triplicate, and the average value of the three tests was calculated.

[0068] The inorganic oxide nanosensors of Example 1, Example 2 and Comparative Example 1 were used as the sample to be tested, and a static magnetic field of 200-300 mT was applied and no magnetic field was applied to obtain the DPPH free radical scavenging rate, which is recorded in Table 1, and a column chart is drawn as shown in Figure 1 The column chart shows that SMF represents a static magnetic field, "+" represents a magnetic field, "-" represents no magnetic field, and the marks at the top of the column of the column chart are error bars.

[0069] Table 1 Record of free radical scavenging rate of nanosensors of Example 1, 2 and Comparative Example 1 in a static magnetic field

[0070] condition Example 1 Example 2 Comparative Example 1 0mT 64.07％ 61.56％ 41.65％ 200~300mT 71.85％ 69.68％ 44.13％

[0071] From Figure 1As can be seen from Table 1, compared with the case without adsorption of nanoselenium, the antioxidant capacity of cerium oxide nanozyme after adsorption of nanoselenium in a magnetic field is more improved; when the molar ratio of nanoselenium to cerium oxide nanozyme reaches 1:5, the antioxidant capacity decreases instead, indicating that the adsorption amount of nanoselenium will affect the antioxidant capacity of nanozyme.

[0072] Test Example 2

[0073] The inorganic oxide-based nanozymes of Example 4 and Comparative Example 2 were used as test samples, and the test method was the same as that of Test Example 1. The DPPH free radical scavenging rate was obtained, and the results were recorded in Table 2. A bar graph was drawn as shown in FIG. Figure 2 As shown in the figure, SMF represents the static magnetic field, "+" represents the presence of a magnetic field, "-" represents the absence of a magnetic field, and the mark at the top of the bar graph is the error bar.

[0074] Table 2 Free radical scavenging rate of nanozymes in Example 4 and Comparative Example 2 in a static magnetic field

[0075] condition Example 4 Comparative Example 2 0mT 51.51％ 46.26％ 200~300mT 67.43％ 48.55％

[0076] from Figure 2 As can be seen from Table 2, compared with the case without nano-selenium adsorption, the antioxidant capacity of manganese oxide nanozyme after nano-selenium adsorption is more improved in the magnetic field, indicating that the adsorption of nano-selenium can enhance the regulatory effect of the magnetic field on the antioxidant capacity of manganese oxide nanozyme.

[0077] Test Example 3

[0078] The inorganic oxide-based nanozymes of Example 1 and Comparative Example 1 were used as test samples. The test method was the same as that of Test Example 1, except that the magnetic field was an alternating magnetic field with a frequency of 250 kHz and a magnetic field strength of 80 mT. The DPPH free radical scavenging rate was obtained, and the results were recorded in Table 3. A bar graph was drawn as shown in FIG. Figure 3 As shown in the figure, AMF represents alternating magnetic field, "+" represents the presence of magnetic field, "-" represents the absence of magnetic field, and the mark at the top of the bar graph is the error bar.

[0079] Table 3 Free radical scavenging rate of nanozymes in Example 1 and Comparative Example 1 in an alternating magnetic field

[0080] condition Example 1 Comparative Example 1 0mT 64.07％ 41.65％ 80mT alternating magnetic field 67.72％ 43.22％

[0081] from Figure 3 As can be seen from Table 3, compared with the case without adsorption of nano-selenium, the antioxidant capacity of cerium oxide nanozyme after adsorption of nano-selenium is more improved in the alternating magnetic field, indicating that the adsorption of nano-selenium can enhance the regulatory effect of the alternating magnetic field on the antioxidant capacity of cerium oxide nanozyme.

[0082] Test Example 4

[0083] The inorganic oxide-based nanozymes of Examples 1, 3 and Comparative Example 1 were used as test samples. The test method was the same as that of Test Example 1, except that only the DPPH radical scavenging rate when a magnetic field was applied was tested. The results are recorded in Table 4 and a bar graph is drawn as shown in FIG. Figure 4 As shown, the marks at the top of the bar graph are error bars.

[0084] Table 4 Free radical scavenging rate records of CeO2 with different Se adsorption amounts in a static magnetic field

[0085] condition Example 1 Example 3 Comparative Example 1 <![CDATA[Se:CeO2摩尔比]]> 1:10 1:20 / 200~300mT 71.85％ 56.71％ 44.13％

[0086] from Figure 4 As can be seen from Table 4, cerium oxide nanozymes with different nano-selenium adsorption amounts have different antioxidant abilities in the same magnetic field; in practical applications, the appropriate nano-selenium adsorption amount can be selected according to needs.

[0087] Test Example 5

[0088] The inorganic oxide-based nanozyme of Example 1 was used as the test sample. The test method was the same as that of Test Example 1, except that the applied magnetic field strength was 0 mT, 50-100 mT, and 200-300 mT, respectively. The DPPH free radical scavenging rate results were recorded in Table 5, and a bar graph was drawn as shown in FIG. Figure 5 As shown, the marks at the top of the bar graph are error bars.

[0089] Table 5 Free radical scavenging rate of nanozymes in different magnetic fields of Example 1

[0090] condition Example 1 0mT 64.07％ 50~100mT 67.40％ 200~300mT 71.85％

[0091] from Figure 5 As can be seen from Table 5, the antioxidant capacity of the inorganic oxide-based nanozyme provided in Example 1 increases with the increase of magnetic field intensity; the intensity of the magnetic field can be adjusted according to actual needs.

[0092] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. An inorganic oxide-based nanozyme, comprising an inorganic oxide nanozyme and nano-selenium adsorbed on the surface of the inorganic oxide nanozyme; the lattice of the inorganic oxide nanozyme contains oxygen vacancies; the molar ratio of the nano-selenium to the inorganic oxide nanozyme is 1:(5-20).

2. The inorganic oxide-based nanozyme according to claim 1, characterized in that The molar ratio of the nano-selenium to the inorganic oxide nanozyme is 1:(10-15).

3. The inorganic oxide-based nanozyme according to claim 1 or 2, characterized in that The inorganic oxide nanozyme includes nano-cerium oxide, nano-manganese oxide or nano-copper oxide.

4. The method for preparing the inorganic oxide-based nanozyme according to any one of claims 1 to 3, comprising: The nanoselenium suspension is mixed with the inorganic oxide nanozyme for in situ adsorption to obtain the inorganic oxide-based nanozyme.

5. The preparation method according to claim 4, characterized in that The in-situ adsorption time is 10 to 20 hours.

6. The preparation method according to claim 4, characterized in that The concentration of nano-selenium in the nano-selenium suspension is 30-250 μg / mL.

7. The preparation method according to claim 6, characterized in that The nano-selenium suspension is prepared by a chemical reduction method, a biosynthesis method or a physical preparation method.

8. A method for regulating the antioxidant capacity of inorganic oxide-based nanozymes, characterized in that: A magnetic field is applied to the inorganic oxide-based nanozyme, wherein the inorganic oxide-based nanozyme is the inorganic oxide-based nanozyme according to any one of claims 1 to 3 or the inorganic oxide-based nanozyme prepared by the preparation method according to any one of claims 4 to 7.

9. The adjustment method according to claim 8, characterized in that: The intensity of the magnetic field is no more than 2000 mT.

10. The adjustment method according to claim 8 or 9, characterized in that: The magnetic field is a static magnetic field or an alternating magnetic field.

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