Lipid-lowering and weight-losing composition with homology of medicine and food as well as preparation method and application thereof

Abstract

The invention discloses a lipid-lowering and weight-losing composition with homology of medicine and food as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine compositions. The composition for reducing fat and losing weight comprises the following raw materials in parts by weight: 10-40 parts of lotus leaves, 20-50 parts of haws, 15-45 parts of poria cocos, 5-35 parts of dried orange peel, 60-130 parts of raspberries, 0-15 parts of fingered citron and 0-15 parts of kudzuvine roots. Compared with a model group, the fat-reducing and weight-losing composition disclosed by the invention has the advantages that the weight of a mouse can be reduced by 12%-15%, the serum total cholesterol level and the leptin level are reduced, the number of fat cells per unit area is increased, the liver adiposis symptom is obviously improved, the expression of heat production factors in epididymal fat and groin fat is up-regulated, and liver toxicity is avoided. The raw materials of the composition for reducing fat and losing weight are all traditional Chinese medicines with homology of medicine and food, so that the composition has no obvious damage to organisms and is safer; the fat-reducing and weight-losing composition can achieve a good weight-losing effect by compounding the raw materials.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine compositions, and in particular relates to a lipid-lowering and weight-loss composition having both medicinal and edible properties, and a preparation method and application thereof. Background Art

[0002] With the changes in lifestyle and dietary structure in modern society, the incidence of obesity, hyperlipidemia and related metabolic diseases has been rising year by year and has become a global public health problem. In the field of chemical drugs, Western medicine lipid-lowering drugs are mainly statins, fibrates, cholesterol absorption inhibitors (such as ezetimibe) and pancreatic lipase inhibitors (such as orlistat). Although these drugs are effective in lowering low-density lipoprotein cholesterol or triglycerides, long-term use may be accompanied by side effects such as muscle toxicity, abnormal liver function, and gastrointestinal reactions. For example, statins can cause myalgia or increased creatine kinase in about 5%-10% of patients, while orlistat may cause steatorrhea and fat-soluble vitamin absorption disorders, seriously affecting patient compliance. In addition, existing drugs mostly target a single target (such as HMG-CoA reductase, PPARα receptor), and their ability to regulate multiple links of lipid metabolism is insufficient, making it difficult to achieve synergistic improvements in multiple indicators such as weight, blood lipids, and insulin resistance.

[0003] In the field of natural medicine, traditional Chinese medicine formulas, due to their multi-ingredient and multi-target effects, have gradually become a key research focus for lipid-lowering and weight-loss. For example, a traditional Chinese medicine composition with weight-loss efficacy, its preparation method, and its use, the specification of which states that the invention uses a combination of five-fingered ginseng, Poria cocos, coix seed, hawthorn, licorice, lotus leaf, cassia seed, Panax notoginseng root, bitter melon, tangerine peel, and Yunnan black tea, which can reduce the weight of SD rats by 9.47%. A topical essence with weight-loss and spleen-strengthening and dampness-removing properties, containing Poria cocos, astragalus, Euryale ferox, cinnamon, hawthorn, licorice, tangerine peel, and Ganoderma lucidum, can reduce the weight of SD rats by 9.03% after 30 days of spraying. A traditional Chinese medicine composition for weight loss and its preparation method, using an aqueous extract of raw astragalus, processed atractylodes, cinnamon, tangerine peel, lotus leaf, raw hawthorn, kelp, turmeric, stir-fried radish seeds, and raw pollen, can achieve a weight loss rate of 10.46% when administered orally to SD rats for 8 weeks. However, the weight loss effects of these traditional Chinese medicine formulas in the prior art still need to be improved. Therefore, it is particularly important to provide a product that is safer than Western medicine, has fewer toxic and side effects, and can also achieve good weight loss effects. Summary of the Invention

[0004] In view of this, the object of the present invention is to provide a lipid-lowering and weight-loss composition that is both medicinal and edible, has no obvious damage to the body, has little toxic and side effects, and can achieve a good weight-loss effect.

[0005] Another object of the present invention is to provide a method for preparing the lipid-lowering and weight-loss composition.

[0006] Another object of the present invention is to provide a use of the lipid-lowering and weight-loss composition in the preparation of lipid-lowering and weight-loss products.

[0007] Another object of the present invention is to provide a lipid-lowering and weight-loss product.

[0008] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0009] The invention provides a lipid-lowering and weight-loss composition with medicinal and edible properties. The lipid-lowering and weight-loss composition comprises the following raw materials in parts by weight: 10 to 40 parts of lotus leaves, 20 to 50 parts of hawthorns, 15 to 45 parts of poria cocos, 5 to 35 parts of tangerine peels, 60 to 130 parts of raspberries, 0 to 15 parts of bergamots, and 0 to 15 parts of kudzu roots.

[0010] The present invention also provides a method for preparing the lipid-lowering and weight-loss composition, which comprises: mixing raw materials by weight, extracting with water, concentrating and drying the filtrate to obtain the lipid-lowering and weight-loss composition.

[0011] Preferably, the mass volume ratio of the raw material to water is 1 g: (12-18) mL.

[0012] Preferably, the extraction method comprises: heating to boiling and then cooking for 10 to 20 minutes.

[0013] Preferably, the extraction is performed 1 to 3 times.

[0014] Preferably, the concentration method comprises rotary evaporation under reduced pressure.

[0015] Preferably, the drying method comprises vacuum freeze drying.

[0016] The present invention also provides a use of the lipid-lowering and weight-loss composition in preparing a lipid-lowering and weight-loss product.

[0017] The present invention also provides a lipid-lowering and weight-loss product, which comprises the lipid-lowering and weight-loss composition and auxiliary materials.

[0018] Preferably, the dosage form of the product includes granules, tablets, powders, capsules, oral solutions, injections, sprays, aerosols, powder sprays, emulsions, nasal drops, suppositories, pills, microsphere preparations or tinctures.

[0019] Beneficial effects of the present invention:

[0020] The present invention provides a lipid-lowering and weight-loss composition that is both medicinal and edible. In a mouse experiment, mice taking the lipid-lowering and weight-loss composition of the present invention had a significant weight reduction, with a weight reduction of 12%-15% compared to the model group. The serum total cholesterol level and leptin level were significantly reduced compared to the model mice, the number of fat cells per unit area was significantly increased compared to the model mice, the symptoms of liver steatosis were significantly improved compared to the model mice, the expression of thermogenic factors in epididymal fat and inguinal fat was significantly upregulated compared to the model mice, and no liver toxicity was observed. The present invention combines extracts of multiple Chinese medicinal herbs with weight-loss effects that are both medicinal and edible in a reasonable proportion. The resulting product is safer and has fewer toxic and side effects than Western medicine, while also achieving a good weight-loss effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 The following are photos of the medicinal and edible raw materials used in the examples;

[0022] Figure 2 The weight changes of mice during the drug administration period in Experimental Example 1; compared with the model group, ** represents P < 0.01, *** represents P < 0.001; compared with the blank group, ### represents P < 0.001;

[0023] Figure 3 Serum AST levels of mice in Experimental Example 1; compared with the model group, ns represents P>0.05, ** represents P<0.01;

[0024] Figure 4 is the serum ALT level of mice in Experimental Example 1; compared with the model group, ns represents P>0.05, ** represents P<0.01;

[0025] Figure 5 is the total cholesterol content in the serum of mice in Experimental Example 1; compared with the blank group, ### represents P < 0.001; compared with the model group, *** represents P < 0.001;

[0026] Figure 6 is the serum leptin content of mice in Experimental Example 1; compared with the blank group, ## represents P < 0.01; compared with the model group, *** represents P < 0.001;

[0027] Figure 7 This is the H&E stained section of mouse liver tissue in Experimental Example 1;

[0028] Figure 8 This is an H&E-stained section of mouse epididymal adipose tissue in Experimental Example 1;

[0029] Figure 9 is the number of epididymal adipocytes per unit area in mice in Experimental Example 1; compared with the blank group, ### represents P < 0.001; compared with the model group, ** represents P < 0.01;

[0030] Figure 10 This is an H&E-stained section of the inguinal adipose tissue of the mouse in Experimental Example 1;

[0031] Figure 11 The number of inguinal fat cells per unit area in mice in Experimental Example 1; compared with the blank group, ## represents P < 0.01; compared with the model group, * represents P < 0.05;

[0032] Figure 12 The expression of TMEM26 in epididymal adipose tissue of mice in Experiment 1; compared with the blank group, # represents P < 0.05; compared with the model group, *** represents P < 0.001;

[0033] Figure 13 The expression of Slc27a1 factor in the inguinal adipose tissue of mice in Experimental Example 1; compared with the blank group, ## represents P < 0.01; compared with the model group, *** represents P < 0.001. DETAILED DESCRIPTION

[0034] The invention provides a lipid-lowering and weight-loss composition with medicinal and edible properties. The lipid-lowering and weight-loss composition comprises the following raw materials in parts by weight: 10 to 40 parts of lotus leaves, 20 to 50 parts of hawthorns, 15 to 45 parts of poria cocos, 5 to 35 parts of tangerine peels, 60 to 130 parts of raspberries, 0 to 15 parts of bergamots, and 0 to 15 parts of kudzu roots.

[0035] In the present invention, the weight portion of the lotus leaf is preferably 10 to 40 parts, for example, 10, 15, 20, 25, 30, 35 or 40 parts; the weight portion of the hawthorn is preferably 20 to 50 parts, for example, 20, 25, 30, 35, 40, 45 or 50 parts; the weight portion of the poria is preferably 15 to 45 parts, for example, 15, 20, 25, 30, 35, 40 or 45 parts; the weight portion of the tangerine peel is preferably 5 to 35 parts, for example, 5, 10 , 15, 20, 25, 30 or 35 parts; the weight portion of raspberry is preferably 60 to 130 parts, for example 60, 70, 80, 90, 100, 110, 120 or 130 parts; the weight portion of bergamot is preferably 0 to 15 parts, for example 0, 1, 3, 4, 5, 7, 9, 11, 13 or 15 parts; the weight portion of kudzu root is preferably 0 to 15 parts, for example 0, 1, 3, 4, 5, 7, 9, 11, 13 or 15 parts.

[0036] The raw materials of the lipid-lowering and weight-loss composition of the present invention are all Chinese medicinal herbs of the same origin as food. Raspberry tastes sweet, sour, and slightly warm; it enters the liver and kidney meridians. It has the effects of tonifying the kidney and consolidating essence, reducing urination and stopping nocturnal emission, and promoting water metabolism by regulating kidney qi, thereby improving the metabolic sluggishness of kidney-deficiency type obesity (such as soreness of the waist and knees, and edema of the lower limbs). Targeting the "kidney deficiency dysfunction" that is the root cause of obesity, the composition is based on warming and tonifying kidney qi to enhance metabolic power; hawthorn tastes sour, sweet, and slightly warm; it enters the spleen, stomach, and liver meridians. The sour taste helps digestion and relieves stagnation, promotes gastrointestinal motility, and relieves stagnation type obesity. Lotus leaf tastes bitter, astringent, and neutral; it enters the liver, spleen, and stomach meridians. It raises the clear and lowers the turbid, improving the heaviness of the head and body and sticky stool caused by phlegm and dampness. Hawthorn "eliminates" stagnation, and lotus leaf "eliminates" dampness and turbidity, and their synergistic effect strengthens the metabolic regulation function; Poria cocos tastes sweet, light, and neutral; it enters the heart, spleen, and kidney meridians. It strengthens the spleen, dispels dampness, promotes diuresis, and reduces swelling, specifically targeting obesity caused by spleen deficiency and dampness excess (e.g., puffiness and muscle flab). Tangerine peel and bergamot have a pungent, bitter, and warming nature, and enter the spleen and lung meridians. They regulate qi and strengthen the spleen, eliminate dampness and resolve phlegm, and promote the overall efficacy of the formula. They direct the herbs to the middle jiao (spleen and stomach), harmonizing the "clearing" properties of lotus leaf with the "turbidity-reducing" properties of poria cocos. Pueraria root has a sweet, pungent, and cooling nature, and enters the spleen, stomach, and lung meridians, relieving muscle tension, reducing fever, promoting fluid production, and quenching thirst, enhancing the kidney-warming and qi-invigorating properties of raspberry. These herbs work together to achieve excellent lipid-lowering and weight-loss effects.

[0037] The lipid-lowering and weight-loss composition of the present invention does not cause significant damage to the body and is safer. The lipid-lowering and weight-loss composition of the present invention can achieve a good weight-loss effect by compounding various raw materials. In a mouse experiment, the weight of mice taking the lipid-lowering and weight-loss composition of the present invention was significantly reduced, with a weight loss of 12%-15% compared to the model group. The serum total cholesterol level and leptin level were significantly reduced compared to the model mice. The number of fat cells per unit area was significantly increased compared to the model mice. The symptoms of liver steatosis were significantly improved compared to the model mice. The expression of thermogenic factors in epididymal fat and inguinal fat was significantly upregulated compared to the model mice, and no liver toxicity was observed. This shows that the lipid-lowering and weight-loss composition of the present invention has a good weight-loss effect and is safe and effective.

[0038] The present invention also provides a method for preparing the lipid-lowering and weight-loss composition, which preferably comprises: mixing raw materials by weight, extracting with water, concentrating and drying the filtrate to obtain the lipid-lowering and weight-loss composition.

[0039] In the present invention, the mass volume ratio of the raw material to water is preferably 1 g: (12-18) mL, for example 1 g: 12 mL, 1 g: 13 mL, 1 g: 14 mL, 1 g: 15 mL, 1 g: 16 mL, 1 g: 17 mL or 1 g: 18 mL. The water is preferably drinking water or distilled water.

[0040] In the present invention, the extraction method preferably includes heating extraction; in some embodiments, the extraction method preferably includes: heating to boiling and then cooking for 10 to 20 minutes, such as 10, 15 or 20 minutes.

[0041] In the present application, the number of extraction is preferably 1-3 times, such as 1, 2 or 3 times; when the number of extraction is 2 or 3 times, the filtrate is combined, and then concentrated and dried.

[0042] In the present application, the method of concentration preferably comprises rotary evaporation under reduced pressure; the temperature of rotary evaporation under reduced pressure is preferably 50-60℃, such as 50, 51, 52, 53, 54, 55, 56, 57, 58, 59 or 60℃, and the rotation speed of rotary evaporation under reduced pressure is preferably 30-50rpm, such as 30, 35, 40, 45 or 50rpm. In the present application, the method of drying preferably comprises vacuum freeze-drying; the temperature of vacuum freeze-drying is preferably -60--80℃, such as -60, -65, -70, -75 or -80℃, the vacuum degree of vacuum freeze-drying is preferably 10-60Pa, such as 10, 20, 30, 40, 50 or 60Pa, and the time of vacuum freeze-drying is preferably 36-60h, such as 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58 or 60h.

[0043] The present application also provides a use of the lipid-lowering and weight-reducing composition in the preparation of a lipid-lowering and weight-reducing product.

[0044] In the present application, the type of product is not particularly limited, and preferably comprises food or medicine; the product can further comprise acceptable adjuvants or aids.

[0045] In the present application, the content of the lipid-lowering and weight-reducing composition in the product is not particularly limited, and the mass percentage is preferably 1%-99%, such as 1%, 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% or 99%.

[0046] The present application also provides a lipid-lowering and weight-reducing product, which comprises the lipid-lowering and weight-reducing composition and adjuvants.

[0047] In the present application, the type of adjuvant is not particularly limited, and comprises but is not limited to one or more of antioxidants, preservatives, colorants, flavoring agents, stabilizers, emulsifiers and acidity regulators.

[0048] In the present application, the dosage form of the product is not particularly limited, and preferably comprises granules, tablets, powders, capsules, oral liquids, injections, sprays, aerosols, powder sprays, emulsions, nasal drops, suppositories, pills, microspheres, or tinctures.

[0049] The technical solutions provided by the present application will be described in detail below in conjunction with examples, but they should not be understood as limiting the scope of protection of the present application.

[0050] In the following examples, unless otherwise specified, all methods are conventional.

[0051] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0052] In the following examples, the lotus leaves, hawthorn, Poria cocos, tangerine peel, raspberry, bergamot and kudzu root used are as follows: Figure 1 shown.

[0053] Example 1

[0054] A lipid-lowering and weight-loss composition having both medicinal and edible properties, comprising the following raw materials in parts by weight: 10 parts of lotus leaves, 20 parts of hawthorn, 15 parts of poria cocos, 5 parts of dried tangerine peel, and 60 parts of raspberry;

[0055] The preparation method of the lipid-lowering and weight-loss composition comprises the following steps:

[0056] The raw materials were washed and mixed according to the above weight proportions, placed in a container, and drinking water was added at a material-liquid ratio of 1 g:12 mL. The mixture was boiled for 10 minutes and filtered to obtain the supernatant. The filter residue was added with drinking water and the mixture was repeated once, and the two filtrates were combined. After rotary evaporation under reduced pressure at 50° C. and 50 rpm, the mixture was dried at -80° C. and a vacuum degree of 60 Pa in a vacuum freeze dryer for 36 hours to obtain a lipid-lowering and weight-loss composition.

[0057] Example 2

[0058] A lipid-lowering and weight-loss composition having both medicinal and edible properties, prepared from the following raw materials in parts by weight: 40 parts of lotus leaves, 50 parts of hawthorn, 45 parts of poria cocos, 35 parts of tangerine peel, and 130 parts of raspberry;

[0059] The preparation method of the lipid-lowering and weight-loss composition comprises the following steps:

[0060] The raw materials were washed and mixed according to the above weight proportions, placed in a container, and drinking water was added at a material-liquid ratio of 1 g:18 mL. The mixture was boiled for 20 minutes and filtered to obtain the supernatant. The filter residue was added with drinking water and the mixture was repeated once, and the two filtrates were combined. After rotary evaporation under reduced pressure at 60° C. and 30 rpm, the mixture was dried in a vacuum freeze dryer at -60° C. and a vacuum degree of 10 Pa for 60 hours to obtain a lipid-lowering and weight-loss composition.

[0061] Example 3

[0062] A lipid-lowering and weight-loss composition with medicinal and edible properties, which is different from Example 1 in that it is made from the following raw materials in parts by weight: 10 parts of lotus leaves, 20 parts of hawthorn, 15 parts of poria cocos, 5 parts of tangerine peel, 60 parts of raspberry, 5 parts of bergamot and 5 parts of kudzu root.

[0063] Example 4

[0064] A medicinal and edible lipid-reducing composition, which is different from example 2, is prepared from the following raw materials by weight: lotus leaf 40 parts, hawthorn 50 parts, fomes 45 parts, dried tangerine or orange peel 35 parts, raspberry 130 parts, bergamot 15 parts and kudzu root 15 parts.

[0065] Example 5

[0066] A medicinal and edible lipid-reducing composition is prepared from the following raw materials by weight: lotus leaf 20 parts, hawthorn 30 parts, fomes 30 parts, dried tangerine or orange peel 20 parts and raspberry 90 parts;

[0067] The preparation method of the lipid-reducing composition, the steps are as follows:

[0068] According to the above weight, wash and mix each raw material, add drinking water in the container, the ratio of material to liquid is 1g:15mL, continue to cook for 20 minutes after boiling, filter the supernatant; add drinking water to the filter residue and repeat once, combine the filtrate of the two times; after rotary evaporation under the condition of 55℃, 40rpm and reduced pressure, dry it in a vacuum freeze dryer at-80℃, vacuum degree 50Pa for 48h, obtain the lipid-reducing composition.

[0069] Example 6

[0070] A medicinal and edible lipid-reducing composition, which is different from example 5, is prepared from the following raw materials by weight: lotus leaf 20 parts, hawthorn 30 parts, fomes 30 parts, dried tangerine or orange peel 20 parts, raspberry 90 parts, bergamot 7 parts and kudzu root 7 parts.

[0071] Comparative example 1

[0072] A medicinal and edible lipid-reducing composition is prepared from the following raw materials by weight: lotus leaf 20 parts, hawthorn 30 parts, fomes 30 parts, dried tangerine or orange peel 20 parts and milkweed 90 parts;

[0073] The preparation method of the lipid-reducing composition, the steps are as follows:

[0074] According to the above weight, wash and mix each raw material, add drinking water in the container, the ratio of material to liquid is 1g:15mL, continue to cook for 20 minutes after boiling, filter the supernatant; add drinking water to the filter residue and repeat once, combine the filtrate of the two times; after rotary evaporation under the condition of 55℃, 40rpm and reduced pressure, dry it in a vacuum freeze dryer at-80℃, vacuum degree 50Pa for 48h, obtain the lipid-reducing composition.

[0075] Comparative example 2

[0076] A medicinal and edible lipid-reducing composition is prepared from the following raw materials by weight: lotus leaf 50 parts, hawthorn 10 parts, fomes 10 parts, dried tangerine or orange peel 45 parts, raspberry 140 parts, bergamot 20 parts and kudzu root 20 parts;

[0077] The preparation method of the lipid-lowering and weight-loss composition comprises the following steps:

[0078] The raw materials were washed and mixed according to the above weight proportions, placed in a container, and drinking water was added at a material-liquid ratio of 1 g:15 mL. The mixture was boiled for 20 minutes and filtered to obtain the supernatant. The filter residue was added with drinking water and the mixture was repeated once, and the two filtrates were combined. After rotary evaporation under reduced pressure at 55° C. and 40 rpm, the mixture was dried in a vacuum freeze dryer at -80° C. and a vacuum degree of 50 Pa for 48 hours to obtain a lipid-lowering and weight-loss composition.

[0079] Comparative Example 3

[0080] A lipid-lowering and weight-loss composition with medicinal and edible properties, prepared from the following raw materials in parts by weight: 20 parts of lotus leaves, 30 parts of hawthorn, 30 parts of poria cocos, 20 parts of tangerine peel, and 90 parts of raspberry;

[0081] The preparation method of the lipid-lowering and weight-loss composition comprises the following steps:

[0082] The raw materials were washed and mixed according to the above weight proportions, placed in a container, and drinking water was added at a material-liquid ratio of 1 g:10 mL. The mixture was boiled for 30 minutes and filtered to obtain the supernatant. The filter residue was added with drinking water and the mixture was repeated once, and the two filtrates were combined. After rotary evaporation under reduced pressure at 40° C. and 30 rpm, the mixture was dried at -50° C. and a vacuum degree of 70 Pa in a vacuum freeze dryer for 30 hours to obtain a lipid-lowering and weight-loss composition.

[0083] Comparative Example 4

[0084] Wash the raspberries, place them in a container, add drinking water, and the solid-liquid ratio is 1g:15mL. After boiling, continue cooking for 20 minutes, filter and take the supernatant; add drinking water to the filter residue and repeat once, combine the two filtrates; after reduced pressure rotary evaporation at 55°C and 40rpm, use a vacuum freeze dryer to dry at -80°C and a vacuum degree of 50Pa for 48 hours to obtain the raspberry extract.

[0085] Test Example 1

[0086] 1. Instruments and equipment

[0087] Rotary evaporator; freeze dryer; powder grinder; reflux extraction device; electronic balance; Zeiss stereo microscope; low-temperature centrifuge; -80℃ refrigerator; fluorescence quantitative PCR instrument; aspartate aminotransferase (AST) detection kit; alanine aminotransferase (ALT) detection kit; total cholesterol detection kit; leptin ELISA kit.

[0088] 2. Mouse Source

[0089] SPF-grade, healthy, mature, 6-8-week-old C57BL / C male mice were purchased and grouped after 1 week of adaptive feeding.

[0090] 3. Drug configuration

[0091] Experimental drug: The lipid-lowering and weight-loss composition of Example 5 was dissolved in 0.5% sodium carboxymethylcellulose solution to prepare a solution with a concentration of 75 mg / mL to obtain the experimental drug;

[0092] Positive drug: Shuljia Orlistat Capsules. When dispensing, open the capsule and take out the powder inside. Use 0.5% sodium carboxymethyl cellulose solution to dissolve it and prepare a solution with a concentration of 5.4 mg / mL to obtain the positive drug.

[0093] 4. Model building and grouping

[0094] The mice were randomly divided into 4 groups, namely blank group, model group, formula group, and orlistat group, with 6 mice in each group. The blank group was given maintenance feed (10% fat), and the model group, formula group, and orlistat group were given high-fat feed (60% fat). The mice were weighed every 3 days until the body weight of the model group, formula group, and orlistat group showed stable and significant differences compared with the blank group, indicating that the model was successfully established.

[0095] 5. Dosing and body weight observation of mice

[0096] After successful modeling, the blank group continued to be fed with maintenance feed (10% fat), and the model group, formula group and orlistat group continued to be fed with high-fat feed (60% fat). At the same time, the formula group was gavaged with the above experimental drugs every day at a dose of 750 mg / kg / d, and the orlistat group was gavaged with the above positive drugs at a dose of 54 mg / kg / d. The blank group and the model group were gavaged with an equal amount of 0.5% sodium carboxymethylcellulose solution. The drugs were administered for 10 weeks, and the weights were weighed once a week and the results were recorded. Figure 2 All statistical analyses were performed using SPSS, Origin, and AI software. Multiple group comparisons were performed using one-way analysis of variance (ANOVA). Data were expressed as the mean ± standard deviation (SD) of independent experiments. P < 0.05 was considered statistically significant. The following experiments were the same.

[0097] Depend on Figure 2The results show that the weight of mice in the formula group decreased rapidly after administration and was maintained at a significantly lower level than that of the model group within 10 weeks. At the 10th week, the weight of mice in the formula group was 30.29±1.98g, and the weight of mice in the model group was 35.50±1.89g. Compared with the model group, the weight loss rate of mice in the formula group was 14.65±5.59%, and the weight loss in the formula group was more significant than that in the orlistat group (the weight of mice in the orlistat group at the 10th week was 31.17±2.92g), indicating that the lipid-lowering and weight-loss composition of the present invention can significantly and stably reduce the weight of mice and achieve better weight loss effects than orlistat.

[0098] 6. Detection of related indicators in mouse serum

[0099] After 10 weeks of administration, all mice were fasted for 12 hours and then killed by carbon dioxide asphyxiation. Whole blood was collected and allowed to stand for 1 hour. The blood was then centrifuged at 3000 rpm for 10 minutes. The supernatant was separated to obtain serum. The AST, ALT, total cholesterol and leptin levels in the serum of each group of mice were detected using an AST kit, an ALT kit, a total cholesterol kit and a leptin ELISA kit, respectively. The results are shown in the figure. Figure 3-Figure 6 shown.

[0100] Depend on Figure 3 、 Figure 4 The results showed that after administration, there were no significant differences in serum AST and ALT levels between the formula group and the model group, indicating that the mice did not experience liver toxicity after taking the lipid-lowering and weight-loss composition of the present invention. However, serum AST and ALT concentrations in the orlistat group were significantly higher than those in the model group, indicating that orlistat-administered model mice experienced significant liver toxicity. This suggests that the lipid-lowering and weight-loss composition of the present invention is safer for the liver than orlistat.

[0101] Depend on Figure 5 The results show that compared with the blank group, the serum total cholesterol level of the model group was significantly increased. After administration, the serum total cholesterol level of the formula group was significantly decreased compared with the model group, indicating that the lipid-lowering and weight-loss composition of the present invention can significantly restore the serum total cholesterol level of obese mice.

[0102] Depend on Figure 6 The results show that compared with the blank group, the serum leptin level of the model group was significantly increased. After administration, the serum leptin level of the formula group was significantly decreased compared with the model group, indicating that the lipid-lowering and weight-loss composition of the present invention can significantly reduce the serum leptin level of obese mice.

[0103] 7. Pathological Observation of Mouse Liver Tissue, Epididymal Adipose Tissue, and Inguinal Adipose Tissue

[0104] The mouse liver tissue, epididymal adipose tissue and inguinal adipose tissue were fixed with 4% paraformaldehyde, paraffin-embedded, routinely sectioned, stained with hematoxylin and eosin (H&E) and observed under an optical microscope to observe the histopathological changes, and the number of epididymal adipocytes per unit area and the number of inguinal adipocytes per unit area were counted, respectively, and the results are shown in Figure 7-11 .

[0105] It can be seen that the liver staining of the model group mice is light and the liver cells are arranged in disorder, accompanied by obvious inflammatory infiltration, indicating that fatty liver has occurred, while the fatty liver phenomenon of the mice in the formula group is obviously improved, and the liver cells are arranged in order Figure 7 ). The volume of epididymal adipose tissue and inguinal adipose tissue of the model group mice is significantly larger than that of the blank group, and the volume of the two kinds of adipose cells of the mice in the formula group is significantly reduced Figures 8-11 ) after administration.

[0106] 8. Expression of TMEM26 factor in epididymal adipose tissue and Slc27a1 factor in inguinal adipose tissue of mice

[0107] Accurately weigh 50 mg of the tissue sample into a 1.5 mL centrifuge tube containing 4 sterile steel balls, add 1 mL of RNAisoPlus reagent, grind in a pre-cooled homogenizer at 60 Hz for 6 min to obtain a tissue homogenate. Extract total RNA and perform reverse transcription to obtain cDNA for subsequent experiments.

[0108] The reagent mixture is subjected to a fluorescent quantitative PCR experiment, GAPDH is used as an internal standard, and the expression amount of the gene is determined by using a 2 -△△CT method, and the results are shown in Figure 12 , Figure 13 .

[0109] It can be seen that the expression levels of TMEM26 factor in epididymal adipose tissue and Slc27a1 factor in inguinal adipose tissue of the formula group are significantly increased compared with the model group, indicating that the lipid-lowering and weight-reducing composition can significantly increase the expression of thermogenic genes in the adipose tissue of mice, thereby accelerating the metabolism of fat to achieve the purpose of lipid-lowering and weight-reducing.

[0110] Test Example 2

[0111] The lipid-lowering and weight-reducing composition prepared by using Example 5, Example 6, Comparative Example 1, Comparative Example 2 and Comparative Example 3, and the raspberry extract of Comparative Example 4 were subjected to a drug administration experiment, and the same method as in Test Example 1 was used to construct a model, administer the mice and observe the body weight, record the body weight of the mice at 10 weeks after administration, and calculate the body weight reduction rate compared with the model group, and the results are shown in Table 1.

[0112] Table 1: Body weight reduction rate of mice in each group

[0113] Grouping Mean ± SD of weight loss rate (%) Example 5 14.6474±5.58923 Example 6 11.9238±6.52665 Comparative Example 1 8.31±2.17652 Comparative Example 2 3.98±1.43972 Comparative Example 3 5.79±2.65714 Comparative Example 4 8.6082±5.15398

[0114] It can be seen that, compared with Comparative Examples 1-4, the lipid-lowering and weight-reducing composition (Example 5, Example 6) of the present application can significantly reduce the weight of mice, Comparative Example 1 replaces raspberry with the same lipid-lowering and weight-reducing Cuscuta chinensis, but the lipid-lowering and weight-reducing effect of the composition is significantly reduced, indicating that the raw materials of the present application cooperate with each other to produce a synergistic effect, thereby improving the lipid-lowering and weight-reducing effect of the composition; Comparative Example 2 adjusts the amount of each raw material, resulting in a significant decrease in the lipid-lowering and weight-reducing effect of the composition, indicating that the use of the raw material ratio of the present application can enable the raw materials to synergize with each other and improve the lipid-lowering and weight-reducing effect; Comparative Example 3 adjusts the condition parameters in the preparation method, and due to the change in parameters, the dissolution of the effective components of each raw material in the composition or the structure of the effective components may be affected, thereby reducing the lipid-lowering and weight-reducing effect of the composition; Comparative Example 4 uses raspberry as a single herb, which has a certain lipid-lowering and weight-reducing effect, but the present application uses a combination of raw materials to achieve a more excellent lipid-lowering and weight-reducing effect. The present application adjusts the raw material ratio and the parameters of the preparation method through the cooperation of each raw material, so that the obtained lipid-lowering and weight-reducing composition can achieve a good weight loss effect.

[0115] The above only describes the preferred embodiments of the present application, and it should be noted that those skilled in the art can make several improvements and refinements without departing from the principles of the present application, and these improvements and refinements should also be considered within the protection scope of the present application.

Claims

1. A lipid-lowering and weight-loss composition that is both medicinal and edible, characterized in that: The lipid-lowering and weight-loss composition comprises the following raw materials in parts by weight: 10-40 parts of lotus leaves, 20-50 parts of hawthorn, 15-45 parts of poria, 5-35 parts of tangerine peel, 60-130 parts of raspberry, 0-15 parts of bergamot and 0-15 parts of kudzu root.

2. The method for preparing the lipid-lowering and weight-loss composition according to claim 1, wherein: The preparation method comprises: mixing raw materials according to weight, adding water for extraction, concentrating and drying the filtrate to obtain the lipid-lowering and weight-loss composition.

3. The preparation method according to claim 2, characterized in that The mass volume ratio of the raw material and water is 1g: (12-18)mL.

4. The preparation method according to claim 2, characterized in that The extraction method comprises: heating to boiling and then boiling for 10 to 20 minutes.

5. The preparation method according to claim 2, characterized in that The extraction is performed 1 to 3 times.

6. The preparation method according to claim 2, characterized in that The concentration method includes rotary evaporation under reduced pressure.

7. The preparation method according to claim 2, characterized in that The drying method includes vacuum freeze drying.

8. Use of the lipid-lowering and weight-loss composition according to claim 1 in the preparation of lipid-lowering and weight-loss products.

9. A lipid-lowering and weight-loss product, characterized in that: The product comprises the lipid-lowering and weight-loss composition according to claim 1 and auxiliary materials.

10. The product according to claim 9, characterized in that The dosage forms of the product include granules, tablets, powders, capsules, oral solutions, injections, sprays, aerosols, powder sprays, emulsions, nasal drops, suppositories, pills, microsphere preparations or tinctures.

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