Traditional Chinese medicine composition for preventing and treating liver rupture syndrome of broiler breeders and application of traditional Chinese medicine composition

Abstract

The invention relates to a traditional Chinese medicine composition for preventing and treating liver rupture syndrome of broiler breeders and application thereof, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition comprises the following raw material medicines in parts by mass: 50-200 parts of silybum marianum, 50-200 parts of schisandra chinensis, 50-200 parts of gentiana macrophylla, 50-200 parts of andrographis paniculata, 50-200 parts of radix paeoniae alba, 50-200 parts of oldenlandia diffusa, 50-200 parts of folium isatidis and 50-200 parts of patrinia. The traditional Chinese medicine composition has the effects of resisting endotoxin, inflammation, allergy and liver injury and the like, and effectively prevents and treats the liver rupture syndrome of the broiler breeders.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for preventing and treating broiler chicken liver rupture syndrome and an application thereof. Background Art

[0002] The information disclosed in this background technology section is only intended to enhance understanding of the overall background of the invention and should not necessarily be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to those skilled in the art.

[0003] Liver rupture syndrome (LRS) is a disease characterized by hepatosplenomegaly, brittle liver, and hepatic rupture with hematochezia in the peritoneum. Based on clinical symptoms, autopsy findings, and histopathological changes, combined with epidemiological investigations conducted by epidemic prevention and control authorities and clinical experience from veterinary pharmaceutical companies, LRS is generally believed to be caused by frequent, excessive, and irregular vaccination with inactivated oil-emulsion vaccines, particularly inactivated infectious rhinitis vaccines, leading to hepatic amyloidosis. The mortality rate can be as high as 15%, and it significantly impacts egg production, causing significant economic losses to chicken farms. Summary of the Invention

[0004] Based on the current state of the art, the present invention aims to provide a Chinese medicine composition for preventing and treating broiler breeder liver rupture syndrome and its application, which can effectively alleviate the symptoms of liver rupture, prevent and treat broiler breeder liver rupture syndrome, and thus reduce the mortality rate of the disease.

[0005] In order to achieve the above object, the technical solution of the present invention is: In the first aspect, a traditional Chinese medicine composition for preventing and treating liver rupture syndrome in broiler chickens comprises the following raw materials in parts by mass: 50-200 parts of milk thistle, 50-200 parts of schisandra chinensis, 50-200 parts of gentiana macrophylla, 50-200 parts of andrographis paniculata, 50-200 parts of white peony root, 50-200 parts of oldenlandia diffusa, 50-200 parts of isatis indigotica and 50-200 parts of patrinia herb.

[0006] In a second aspect, a medicine for preventing and treating broiler chicken liver rupture syndrome comprises the above-mentioned traditional Chinese medicine composition.

[0007] In a third aspect, the preparation method of the above-mentioned drug for preventing and treating broiler chicken liver rupture syndrome comprises: S1. Mix set amounts of Silybum marianum, Schisandra chinensis, Gentiana macrophylla, Andrographis paniculata, White Peony Root, Hedyotis diffusa, Folium Isatidis, and Patrinia patriniae in parts by weight, add water and soak in warm water to obtain a decoction material; S2, decocting the decoction raw materials for 0.5-2 hours, filtering to obtain medicinal residues and the first stock solution; S3, decocting the medicinal residue with water for 0.5-1 h, filtering to obtain a second stock solution; S4. Mixing the first stock solution and the second stock solution.

[0008] A fourth aspect is the use of the above-mentioned Chinese medicine composition and / or medicine in preventing and treating liver rupture syndrome in broiler breeders.

[0009] The beneficial effects of the present invention are: 1. The present invention utilizes Traditional Chinese Medicine (TCM) theory to offer three major advantages. First, it offers the advantage of syndrome differentiation and treatment. TCM theory categorizes broiler chicken liver rupture syndrome as a syndrome of damp-heat in the liver and gallbladder caused by heat and toxicity. The treatment principle is to clear heat and detoxify, clear heat and eliminate dampness, and support vital Qi (nourishing blood, invigorating Qi, nourishing the kidneys, and consolidating Yin). Medications used: Milk thistle clears heat and detoxifies, protects the liver and promotes bile secretion. Hedyotis diffusa, Isatis indigotica, and Patrinia lappa clear heat and detoxify. Andrographis paniculata and Gentiana macrophylla clear heat and eliminate dampness. White peony root nourishes blood and consolidates Yin, while Schisandra chinensis invigorates Qi and nourishes the kidneys. Second, it offers the advantage of syndrome differentiation and treatment. After vaccination with oil-emulsion inactivated vaccines, especially those for rhinitis, the continuous stimulation of endotoxins and chronic inflammation can lead to immune stress, resulting in amyloidosis, and ultimately liver damage and even rupture. The advantage of syndrome differentiation and treatment lies in the balanced consideration of anti-endotoxin, anti-inflammatory, anti-allergic, and anti-liver damage effects. Third, it leverages the advantages of TCM immunology. By applying the classical theories of TCM, combining TCM immunology and TCM immunology theories, and using TCM, we can protect the liver and promote bile secretion, as well as suppress humoral immunity, and thus play a preventive and therapeutic role in chicken liver rupture syndrome.

[0010] 2. The present invention provides a traditional Chinese medicine composition for preventing and treating liver rupture syndrome in broiler chickens, which can take into account the effects of anti-endotoxin, anti-inflammatory, anti-allergic and anti-liver damage. Two traditional Chinese medicines each with anti-endotoxin, anti-inflammatory, anti-allergic and anti-liver damage effects are screened out from 300 commonly used traditional Chinese medicines. In the prevention plan, the most critical thing is to fight endotoxin: therefore, the anti-endotoxin Folium Isatidis and Herba Patriniae are used as the main medicines. Folium Isatidis clears heat and cools blood, and detoxifies, while Herba Patriniae clears heat and detoxifies, and promotes blood circulation and discharges pus; the anti-inflammatory White Peony Root and Herba Hedyotis Diffusae are used as the auxiliary medicines, which nourish blood and regulate menstruation, restrain yin and stop sweating, soften the liver and relieve pain, and suppress liver yang; Herba Hedyotis Diffusae clears heat and detoxifies, and promotes dampness; the anti-allergic Gentiana Macrophylla and Andrographis Paniculata are used as the adjuvants. Gentiana Macrophylla eliminates rheumatism, clears damp-heat, stops pain, and reduces deficiency fever, while Andrographis Paniculata clears heat and detoxifies, purges fire, and dries dampness; Milk thistle and Schisandra chinensis are used as the envoys, which resist liver damage. Milk thistle clears heat and detoxifies, protects the liver, and promotes bile secretion, while Schisandra chinensis is astringent, replenishes qi and produces body fluid, and nourishes the kidney and calms the mind. In the treatment plan, the most critical thing is to resist liver damage. Therefore, milk thistle and schisandra chinensis, which are used to resist liver damage, are used as the main medicines, Qinjiao and Andrographis paniculata, which are used to resist allergies, are used as the auxiliary medicines, white peony root and Houttuynia cordata, which are used to resist inflammation, are used as the adjuvant medicines, and Isatis indigotica and Patrinia chinensis, which are used to resist endotoxins, are used as the guiding medicines.

[0011] 3. The present invention provides a medicine for preventing and treating broiler chicken liver rupture syndrome, which uses multiple Chinese medicinal herbs as raw materials, including milk thistle, schisandra chinensis, gentiana macrophylla, andrographis paniculata, white peony root, oldenlandia diffusa, isatis indigotica, and patrinia herb. It adopts a reasonable processing process and is easy to use. The Chinese medicinal composition can effectively prevent and treat broiler chicken liver rupture syndrome, thereby improving economic benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] The accompanying drawings, which constitute a part of the present invention, are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0013] Figure 1 This is a broken line chart of Experiment 1 in Example 4.

[0014] Figure 2 This is a broken line chart of Experiment 2 in Example 4. DETAILED DESCRIPTION

[0015] It should be noted that the following detailed descriptions are exemplary and intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which the present invention belongs.

[0016] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present invention. As used herein, unless the context clearly indicates otherwise, the singular form is intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, they indicate the presence of features, steps, operations, devices, components and / or combinations thereof.

[0017] One or more embodiments of the present invention provide a traditional Chinese medicine composition for preventing and treating liver rupture syndrome in broiler chickens, comprising the following raw materials in parts by weight: 50-200 parts of milk thistle, 50-200 parts of schisandra chinensis, 50-200 parts of gentiana macrophylla, 50-200 parts of andrographis paniculata, 50-200 parts of white peony root, 50-200 parts of oldenlandia diffusa, 50-200 parts of isatis indigotica, and 50-200 parts of patrinia herb.

[0018] The above Chinese medicine composition is used for prevention and treatment from the perspective of traditional Chinese medicine, and must take into account anti-endotoxin, anti-inflammatory, anti-allergic and anti-liver damage. The anti-endotoxin Chinese medicines selected are Folium Isatidis and Herba Patriniae, the anti-inflammatory Chinese medicines selected are Paeonia lactiflora and Hedyotis diffusa, the anti-allergic Chinese medicines selected are Gentiana macrophylla and Andrographis paniculata, and the anti-liver damage Chinese medicines selected are Milk thistle and Schisandra chinensis. Prevention and treatment plans have been formulated separately. The functions of each raw material in the present invention are as follows: Milk thistle is the achene of the Asteraceae plant Silybum marianum. Its properties are bitter and cooling. Its benefits include clearing heat and detoxifying, protecting the liver, and promoting bile secretion. Its uses include combating liver damage. It has a positive effect on liver and gallbladder disorders, including acute or chronic hepatitis, cirrhosis, fatty liver disease, metabolic-toxic liver damage, cholelithiasis, cholangitis, and perihepatocholangitis. It can rapidly improve biochemical parameters such as serum bilirubin and alanine aminotransferase. The silymarin it contains, also known as simarin, has hepatoprotective and therapeutic effects on liver disease, combats liver poisoning, and has X-ray protection.

[0019] Schisandra chinensis, the dried mature fruit of the Magnoliaceae plant Schisandra chinensis, is commonly known as "Northern Schisandra chinensis." Its properties and flavors are sour, sweet, and warm. It enters the lung, heart, and kidney meridians. Its effects are astringent, invigorating, and nourishing, and it can nourish the kidneys and calm the mind. It is used to treat chronic cough, asthma, nocturnal emission, spermatorrhea, enuresis, frequent urination, chronic diarrhea, spontaneous sweating, night sweats, thirst due to loss of body fluids, internal heat and polydipsia, palpitations, and insomnia. It is also used to treat liver damage.

[0020] Gentiana macrophylla is the dried root of Gentiana macrophylla, Gentiana tasselata, Gentiana scabra, or Gentiana minor, all belonging to the Gentianaceae family. The first three are commonly known as "Qinjiao" and "tasselata," respectively, while the latter is commonly known as "Xiaoqinjiao." Its properties are pungent, bitter, and neutral. It enters the stomach, liver, and gallbladder meridians. It has the effects of dispelling rheumatism, clearing damp-heat, relieving pain, and alleviating asthenic fever. It is used for rheumatic pain, hemiplegia caused by stroke, muscle spasms, joint pain, damp-heat jaundice, hot flashes due to bone steaming, and fever caused by malnutrition in children. It has anti-allergic properties, including anti-anaphylactic shock and antihistamine effects.

[0021] Andrographis paniculata, the whole herb of the Acanthaceae family, has a bitter and cold nature. It enters the Heart, Lung, Large Intestine, and Bladder meridians. Its efficacy is clearing heat and detoxifying, purging fire, and drying dampness. It is used to treat wind-heat colds, fevers caused by febrile diseases, lung-heat coughs, whooping cough, lung abscesses, sore throats, damp-heat jaundice, stranguria, erysipelas, sores, carbuncles, eczema, and snake bites. It has an anti-allergic effect, particularly against endotoxin allergies. Andrographis paniculata has anti-allergic effects against hypersensitivity caused by endotoxins and is used to treat endotoxemia in broiler breeder chickens with liver rupture.

[0022] White peony root is the dried root of the Ranunculaceae plant Paeonia lactiflora. Its properties are bitter, sour, and slightly cold. It enters the Liver and Spleen meridians. Its effects include nourishing blood and regulating menstruation, astringing Yin and stopping sweating, softening the liver and relieving pain, and calming Liver Yang. It is used for sallow complexion caused by blood deficiency, irregular menstruation, spontaneous sweating, night sweats, flank pain, abdominal pain, cramps in the limbs, headaches, and dizziness. It has anti-inflammatory and anti-ulcer properties.

[0023] Hedyotis diffusa is the whole herb, including its roots, of the dicotyledonous plant Hedyotis diffusa, a member of the Rubiaceae family. Its properties are bitter and sweet, cold, and non-toxic. It enters the heart, liver, spleen, and large intestine meridians. Its efficacy is heat-clearing and detoxifying, and it promotes dampness. It is used to treat lung heat, cough, asthma, sore throat, intestinal carbuncle, furuncle, ulcer, snake bite, painful stranguria caused by heat, edema, dysentery, enteritis, and jaundice caused by damp heat. Its use is anti-inflammatory.

[0024] Folium Isatidis (Isatidis indigotica) is a plant of the Cruciferae family (Isatis indigotica), the Acanthaceae family (Isatis indigotica), the Verbenaceae family (Isatis chinensis), and the Polygonum tinctorium (Polygonum indigotica). Its properties are bitter and cold. Its effects are heat-clearing, cooling blood, and detoxifying. Indications: Japanese encephalitis, influenza, mumps, upper respiratory tract infections, pneumonia, acute hepatitis, fever rash, erysipelas, furuncles, and snake bites. Its use is as an anti-endotoxin.

[0025] Patrinia odorata (Papiliopogon chinensis) is the whole herb of Patrinia chrysantha and Patrinia leucophylla, both of the Patrinaceae family. Its properties are pungent, bitter, and slightly cold. It enters the large intestine and liver meridians. Its effects include clearing heat and detoxifying, promoting blood circulation and discharging pus. It is used to treat intestinal abscesses, lung abscesses, carbuncle swelling, dysentery, and postpartum abdominal pain caused by blood stasis. Its use is anti-endotoxin.

[0026] Optionally, the following raw materials are included in parts by mass: 50 parts of milk thistle, 50 parts of schisandra chinensis, 100 parts of gentiana macrophylla, 100 parts of andrographis paniculata, 150 parts of white peony root, 150 parts of white flower hedyotis diffusa, 200 parts of isatis indigotica and 200 parts of patrinia herb; for preventing broiler chicken liver rupture syndrome, in which the most critical factor in the prevention program is anti-endotoxin, followed by anti-inflammatory, followed by anti-allergic, and then anti-liver damage. Therefore, isatis indigotica and patrinia herb are monarch drugs, white peony root and white flower hedyotis diffusa are ministerial drugs, gentiana macrophylla and andrographis paniculata are adjuvant drugs, and milk thistle and schisandra chinensis are guiding drugs.

[0027] Optionally, the drug comprises the following raw materials in parts by mass: 200 parts of milk thistle, 200 parts of schisandra chinensis, 150 parts of gentiana macrophylla, 150 parts of andrographis paniculata, 100 parts of white peony root, 100 parts of oldenlandia diffusa, 50 parts of isatis indigotica and 50 parts of patrinia herb; used for treating broiler liver rupture syndrome, in which the most critical factor in the treatment plan is anti-liver damage, followed by anti-allergy, followed by anti-inflammatory, and then anti-endotoxin; therefore, milk thistle and schisandra chinensis are the monarch drugs, gentiana macrophylla and andrographis paniculata are the minister drugs, white peony root and oldenlandia diffusa are the adjuvant drugs, and isatis indigotica and patrinia herb are the guiding drugs.

[0028] One or more embodiments of the present invention provide a medicine for preventing and treating broiler chicken liver rupture syndrome, comprising the above-mentioned traditional Chinese medicine composition.

[0029] Optionally, pharmaceutically acceptable excipients may be included to facilitate the preparation of dosage forms and the use of the drug.

[0030] Optionally, the dosage form of the drug includes any one of oral solution, granules, fluid extract, tablets and capsules.

[0031] One or more embodiments of the present invention provide a method for preparing the above-mentioned drug for preventing and treating broiler chicken liver rupture syndrome, comprising the following steps: S1. Mix set amounts of Silybum marianum, Schisandra chinensis, Gentiana macrophylla, Andrographis paniculata, White Peony Root, Hedyotis diffusa, Folium Isatidis, and Patrinia patriniae in parts by weight, add water and soak in warm water to obtain a decoction material; S2, decocting the decoction raw materials for 0.5-2 hours, filtering to obtain medicinal residues and the first stock solution; S3, decocting the medicinal residue with water for 0.5-1 h, filtering to obtain a second stock solution; S4. Mixing the first stock solution and the second stock solution.

[0032] Optionally, add warm water to S1 and soak for 1 to 2 hours.

[0033] Optionally, the method includes S5, concentrating the mixed stock solution obtained in S4, adding ethanol, refrigerating and standing, and then filtering to obtain a third stock solution; recovering ethanol from the third stock solution, adding water, filling, and sterilizing to obtain an oral solution; Alternatively, the method includes S6, concentrating the mixed stock solution obtained in S4 to obtain a clear paste, adding auxiliary materials to the clear paste, and drying to obtain granules; and the dosage forms such as oral liquid or granules can be obtained.

[0034] Optionally, in S5, the mixed stock solution is concentrated to a relative density of 1.1-1.2 to become a concentrated stock solution, preferably 1.17; the volume ratio of the added ethanol to the concentrated stock solution is (65-75): (25-35), and the mixture is refrigerated and allowed to stand for more than 48 hours; the amount of water added is 20-30% of the volume of the third stock solution.

[0035] Optionally, in S6, the concentrate is concentrated to a relative density of 1.30-1.33; the added auxiliary materials include sugar and dextrin; and the mass ratio of the clear paste, sugar and dextrin is 1:(2-3):(1-2).

[0036] One or more embodiments of the present invention provide the use of the above-mentioned traditional Chinese medicine composition and / or medicine in preventing and treating liver rupture syndrome in broiler breeders.

[0037] The present invention will be further described below with reference to the embodiments.

[0038] Example 1 The invention discloses an oral liquid of a traditional Chinese medicine composition for preventing liver rupture syndrome in broiler chickens. The oral liquid comprises the following ingredients by mass: 50g of milk thistle, 50g of schisandra chinensis, 100g of gentiana macrophylla, 100g of andrographis paniculata, 150g of white peony root, 150g of oldenlandia diffusa, 200g of isatis indigotica, and 200g of patrinia herb.

[0039] The preparation method of the Chinese medicine oral liquid comprises the following steps: S1: Mix milk thistle, schisandra chinensis, gentiana macrophylla, andrographis paniculata, white peony root, oldenlandia diffusa, isatis indigotica, and patrinia herba, and soak in warm water for 1 hour; S2: Decoction the above 8 ingredients for 1 hour, filter and obtain the first stock solution; S3: decoct the medicinal residue for another 40 min, filter, and obtain the second stock solution; S4: combining the first stock solution and the second stock solution to obtain a combined filtrate; S5. Concentrate the combined filtrate to a relative density of 1.17 (90°C) to form a concentrated stock solution, add ethanol at a volume ratio of 35:65 between the concentrated stock solution and ethanol, refrigerate and let stand for 48 hours, and filter to obtain a third stock solution; recover ethanol from the filtrate of the third stock solution to obtain a filling raw liquid, and then add water at a volume ratio of 4:1 between the filling raw liquid and water for filling, and sterilize to obtain 1000 mL of the Chinese medicine oral solution.

[0040] Example 2 The invention discloses an oral liquid of a traditional Chinese medicine composition for treating broiler chicken liver rupture syndrome, which is composed of the following ingredients by mass: 200g of milk thistle, 200g of schisandra chinensis, 150g of gentiana macrophylla, 150g of andrographis paniculata, 100g of white peony root, 100g of oldenlandia diffusa, 50g of isatis indigotica, and 50g of patrinia herb.

[0041] The preparation method was the same as that in Example 1 to obtain 1000 mL of Chinese medicine oral liquid.

[0042] Example 3 A granule of a traditional Chinese medicine composition for treating broiler liver rupture syndrome, wherein the mass fraction of the raw material is the same as that of Example 2.

[0043] The preparation method comprises: S1: Mix milk thistle, schisandra chinensis, gentiana macrophylla, andrographis paniculata, white peony root, oldenlandia diffusa, isatis indigotica, and patrinia herba, and soak in warm water for 1 hour; S2: Decoction the above 8 ingredients for 1 hour, filter, and obtain the first stock solution; S3: decoct the medicinal residue for another 40 min, filter, and obtain the second stock solution; S4: combining the first stock solution and the second stock solution to obtain a combined filtrate; S6. Concentrate the combined filtrate to a relative density of 1.32±0.01 (20°C) to obtain a clear paste, add sugar and dextrin in a mass ratio of 1:2.5:1 to the clear paste, and dry to obtain granules.

[0044] The difference from Example 2 is that the preparation method does not adopt step S5, but instead prepares particles according to step S6.

[0045] Example 4 Application of Example 1 or Example 2 in preventing and treating broiler chicken liver rupture syndrome.

[0046] Liver rupture syndrome (LRS) in white-feathered broiler breeders is characterized by enlarged liver and spleen, brittle liver, and hemorrhagic peritoneal bleeding. This leads to high batch mortality rates, significantly impacting egg production, with mortality rates as high as 15%. The syndrome is highly correlated with breeder gender: all flocks experiencing LRS were hens, while roosters appeared normal. Late-feather broilers also experience LRS, with symptoms more commonly seen in the slow-feathering D line and milder in the fast-feathering D line, while all other A, B, and C lines appear normal. This correlation is also consistent in the downstream parent stock.

[0047] Based on a comprehensive analysis of clinical symptoms, autopsy findings, and histopathological changes, along with epidemiological investigations conducted by epidemic control authorities and clinical experience from the technical department of a veterinary pharmaceutical company, the etiology of ruptured liver syndrome in broiler breeders was determined to be hepatic amyloidosis caused by frequent, excessive, and irregular vaccination with inactivated oil-emulsion vaccines, particularly inactivated infectious rhinitis vaccines. Amyloidosis is typically a progressive, chronic process, rarely resulting in acute death, except for liver rupture and internal hemorrhage. Amyloid deposits in large quantities in tissues and organs such as the liver, spleen, and intestines, causing the disappearance of some parenchymal cells and their replacement by amyloid. Amyloid infiltration ultimately leads to cell death, tissue destruction, and death. This leads to amyloid production in hepatocytes, which accumulates in the liver, impairing liver morphology and function, leading to organ dysfunction and ultimately death. Previously, there was no effective treatment specifically targeting the high mortality rate associated with ruptured liver syndrome in broiler breeders. Treating ruptured liver syndrome in broiler breeders would address a major challenge in poultry production.

[0048] The inventors conducted a clinical efficacy trial at a large broiler breeder farm. The farm administered a secondary immunization of an inactivated oil-emulsion infectious coryza vaccine 120 days after the start of the vaccination schedule. To address the different objectives of prevention and treatment, they designed two separate trials: one for drug prevention and the other for drug treatment. The mortality rate of broiler breeders was recorded over a total of 10 days, from day 1 to day 8 of drug administration, as well as two days after drug withdrawal.

[0049] Test 1 A clinical efficacy test was conducted on 36,000 broiler breeder chickens in buildings numbered 2, 8, and 13 (12,000 in each building). The test started after the broiler breeder chickens were immunized for the second time, that is, on the 121st day of the immunization program when no symptoms appeared. The test was divided into three groups: Building 2 drank the Chinese herbal composition for prevention in Example 1 (Group 1); Building 8 drank the Chinese herbal composition for treatment in Example 2 (Group 2); and Building 13 served as a control group and did not take any medicine. Groups 1 and 2 drank their respective Chinese herbal compositions for 8 consecutive days, and stopped using them on the 9th and 10th days; then, the mortality of the three groups from the 1st day to the 10th day was counted. Drug dosage: 2.5 ml per chicken, 1000 mL for 400 chickens, and the medication ratio for the whole flock was the same. The oral solution of the drug was added to the drinking water every morning and administered at one time within 2-3 hours. The mortality rate data obtained are shown in Table 1, and the line statistics obtained are shown in Table 1.Figure 1 shown.

[0050] Table 1

[0051] Test 2 A clinical efficacy trial was conducted on 36,000 broiler breeder chickens (12,000 chickens per building) in buildings numbered 17, 18, and 25. Following the second immunization on day 121, the broiler breeders developed symptoms of liver rupture syndrome (LRS). Necropsy revealed a liver rupture rate exceeding 80%, with mortality occurring. The trial began on day 125. The chickens in building 17 were treated with the preventive Chinese herbal composition described in Example 1 (Group 1); the chickens in building 18 were treated with the therapeutic Chinese herbal composition described in Example 2 (Group 2); and the chickens in building 25 served as a control group and received no medication. Groups 1 and 2 each received their respective LRs for 8 consecutive days, discontinuing treatment on days 9 and 10. Mortality rates were then recorded for each of the three groups from day 1 to day 10. The drug dosage was 2.5 ml per bird, with 1000 ml applied to 400 birds. Dosage was consistent across the flock. The oral solution was added to the drinking water every morning and administered within 2-3 hours. The obtained mortality rate data is shown in Table 2, and the obtained broken line statistical graph is shown in Figure 2 shown.

[0052] Table 2

[0053] In Experiment 1, the broiler breeders were tested 121 days after the second vaccination with the inactivated oil-emulsion infectious rhinitis vaccine. In Experiment 2, the broiler breeders were tested 125 days after clinical mortality and liver rupture symptoms were observed after the second vaccination with the inactivated oil-emulsion infectious rhinitis vaccine. It can be seen that Example 1 in Experiment 1 was effective in preventing liver rupture syndrome in broiler breeders and is suitable for use as a preventive drug. In contrast, Example 2 in Experiment 2 was effective in treating broiler breeders already suffering from liver rupture syndrome and is suitable for use as a therapeutic drug.

[0054] The foregoing description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that various modifications and variations of the present invention are possible. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention are intended to be within the scope of protection of the present invention.

Claims

1. A Chinese medicine composition for preventing and treating broiler chicken liver rupture syndrome, characterized in that: The invention comprises the following raw materials in parts by weight: 50-200 parts of milk thistle, 50-200 parts of schisandra chinensis, 50-200 parts of gentiana macrophylla, 50-200 parts of andrographis paniculata, 50-200 parts of white peony root, 50-200 parts of oldenlandia diffusa, 50-200 parts of isatis indigotica and 50-200 parts of patrinia herb.

2. The Chinese medicine composition according to claim 1, wherein The invention comprises the following raw materials in parts by weight: 50 parts of milk thistle, 50 parts of schisandra chinensis, 100 parts of gentiana macrophylla, 100 parts of andrographis paniculata, 150 parts of white peony root, 150 parts of oldenlandia diffusa, 200 parts of isatis indigotica and 200 parts of patrinia herba.

3. The Chinese medicine composition according to claim 1, wherein The invention comprises the following raw materials in parts by weight: 200 parts of Silybum marianum, 200 parts of Schisandra chinensis, 150 parts of Gentiana macrophylla, 150 parts of Andrographis paniculata, 100 parts of White Peony Root, 100 parts of Hedyotis diffusa, 50 parts of Isatis indigotica and 50 parts of Patrinia lappa.

4. A drug for preventing and treating broiler chicken liver rupture syndrome, characterized in that: Including the Chinese medicine composition as claimed in claim 1.

5. The drug according to claim 4, wherein Contains pharmaceutically acceptable excipients; Alternatively, the dosage form of the drug includes any one of oral solution, granules, fluid extract, tablets and capsules.

6. A method for preparing a drug for preventing and treating broiler chicken liver rupture syndrome according to claim 4, characterized in that: The following steps are involved: S1. Mix set amounts of Silybum marianum, Schisandra chinensis, Gentiana macrophylla, Andrographis paniculata, White Peony Root, Hedyotis diffusa, Isatis indigotica, and Patrinia patriniae in parts by weight, add water and soak in warm water to obtain a decoction material; S2, decocting the decoction raw materials for 0.5-2 hours, filtering to obtain medicinal residues and the first stock solution; S3, decocting the medicinal residue with water for 0.5-1 h, filtering to obtain a second stock solution; S4. Mixing the first stock solution and the second stock solution.

7. The preparation method according to claim 6, wherein In S1, add warm water and soak for 1 to 2 hours.

8. The preparation method according to claim 6, wherein The process comprises the steps of: S5, concentrating the mixed stock solution obtained in S4, adding ethanol, refrigerating and standing the mixed stock solution, and filtering the mixed stock solution to obtain a third stock solution; recovering ethanol from the third stock solution, adding water, filling the solution, and sterilizing the solution to obtain an oral solution; Alternatively, the method includes S6, concentrating the mixed stock solution obtained in S4 to obtain a clear paste, adding auxiliary materials to the clear paste, and drying to obtain granules; and the dosage forms such as oral liquid or granules can be obtained.

9. The preparation method according to claim 8, wherein In S5, the mixed stock solution is concentrated to a relative density of 1.1-1.2 to form a concentrated stock solution, preferably 1.17; the volume ratio of ethanol added to the concentrated stock solution is (65-75):(25-35), and the mixture is refrigerated and allowed to stand for more than 48 hours; the amount of water added is 20-30% of the volume of the third stock solution; Alternatively, in S6, the mixture is concentrated to a relative density of 1.30-1.33; the added auxiliary materials include sugar and dextrin; and the mass ratio of the clear paste, sugar and dextrin is 1:(2-3):(1-2).

10. Use of the traditional Chinese medicine composition according to any one of claims 1 to 3 and / or the medicine according to any one of claims 4 to 5 for preventing and treating liver rupture syndrome in broiler breeders.

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