Traditional Chinese medicine composition for nourishing blood and tranquilizing mind and preparation method thereof

Abstract

The invention belongs to the technical field of medicines, and particularly relates to a traditional Chinese medicine composition for nourishing blood and tranquilizing and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following components in parts by weight: 150 parts of deer blood extract, 50 to 60 parts of extract A, 80 to 90 parts of extract B, 120 to 130 parts of starch, 10 to 15 parts of polyvinylpyrrolidone and 1 to 2 parts of magnesium stearate, the extract A is prepared by fermenting semen cuscutae, radix paeoniae alba, radix angelicae sinensis and fructus lycii; the extract B is prepared by performing enzymolysis on spina date seeds, pericarpium citri reticulatae, Chinese yams and white tea. The traditional Chinese medicine composition prepared by the invention has a good treatment effect on inflammatory anemia and iron-deficiency anemia at the same time.

Description

Technical Field

[0001] The invention belongs to the technical field of medicine, and particularly relates to a blood-nourishing and mind-calming traditional Chinese medicine composition and a preparation method thereof. Background Art

[0002] Anemia of inflammation (AI) and iron-deficiency anemia (IDA) are two common types of anemia. Although both involve abnormalities in iron metabolism, their etiology, pathogenesis, clinical manifestations, and diagnostic and treatment approaches differ significantly. The primary cause of iron-deficiency anemia is the depletion of body iron stores, which cannot meet the needs of normal erythropoiesis. This is typically caused by insufficient iron intake, increased demand, or excessive iron loss. Anemia of inflammation, on the other hand, is a type of anemia triggered by a chronic inflammatory state and is commonly seen in conditions such as infection, autoimmune diseases, malignancies, and chronic kidney disease. Its underlying cause is the increased release of proinflammatory cytokines (such as IL-6 and TNF-α), which leads to elevated hepcidin levels, limiting the release of iron stored in macrophages and intestinal iron absorption, resulting in functional iron deficiency.

[0003] Commonly used blood-enriching products are mainly used to improve iron-deficiency anemia, but are not ideal for treating inflammatory anemia. The purpose of this application is to continue exploring the above-mentioned formula, further research and optimize the existing formula, expand the scope of application of the formula, improve the clinical value of related drugs, benefit more patients, and add value to the company.

[0004] Therefore, there is an urgent need for a Chinese medicine composition for nourishing blood and calming the mind and a preparation method thereof. Summary of the Invention

[0005] The present invention aims to provide a Chinese medicinal composition for nourishing blood and calming the mind and a preparation method thereof.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] A traditional Chinese medicine composition for nourishing blood and calming the mind, comprising medicine granules and a composition peel, wherein the medicine granules comprise the following components in parts by weight: 150 parts of deer blood extract, 50-60 parts of extract A, 80-90 parts of extract B, 120-130 parts of starch, 10-15 parts of polyvinyl pyrrolidone, and 1-2 parts of magnesium stearate;

[0008] The extract A is prepared by fermenting Cuscuta seeds, white peony root, Chinese angelica root and wolfberry;

[0009] The extract B is prepared by enzymatic hydrolysis of jujube seeds, dried tangerine peel, Chinese yam and white tea.

[0010] Further, the extract A is prepared by fermenting the Semen cuscutae, Radix paeoniae alba, Angelica sinensis and Fructus lycii in turn using Bacillus subtilis, Bacillus fusiformis and Lactobacillus paracasei.

[0011] Further, the Semen cuscutae, Radix paeoniae alba, Angelica sinensis and Fructus lycii have a weight ratio of (5-8):10:(3~5):(12~14).

[0012] Bacillus subtilis, strain number MCCC1A11421, purchased from China General Microbiological Culture Collection Center.

[0013] Bacillus fusiformis, strain number CICC23677, purchased from China Industrial Microbial Culture Collection Center.

[0014] Lactobacillus paracasei, strain number CICC6107, purchased from China Industrial Microbial Culture Collection Center.

[0015] Further, the preparation method of the extract A comprises the following steps:

[0016] (1) mixing the Semen cuscutae, Radix paeoniae alba, Angelica sinensis and Fructus lycii, crushing to less than 60 mesh to obtain powder A;

[0017] (2) mixing the powder A and water in a weight ratio of 1:(6~8) uniformly to obtain fermentation material; adding Bacillus subtilis first, aerobic fermentation at 35~40℃ for 20~22h, sterilizing, then adding Bacillus fusiformis, anaerobic fermentation at 35~40℃ for 26~28h, sterilizing, then adding Lactobacillus paracasei, anaerobic fermentation at 35~40℃ for 27~29h, sterilizing to obtain fermentation product;

[0018] (3) filtering the fermentation product through a 0.22μm filter membrane, spray drying the filtrate to obtain the extract A.

[0019] The amount of Bacillus subtilis is 10 7 ~10 8 CFU / mL of fermentation material; the amount of Bacillus fusiformis is 10 7 ~10 8 CFU / mL of fermentation material; the amount of Lactobacillus paracasei is 10 8 ~10 9 CFU / mL of fermentation material.

[0020] The present invention adds extracts A and B to a traditional Chinese medicine composition to produce a synergistic effect, improving the treatment effect of iron deficiency anemia. Deer blood extract is rich in heme iron, which can directly participate in the synthesis of hemoglobin, thereby effectively alleviating the symptoms of iron deficiency anemia. By adding extracts A and B, the present invention achieves a good coordination between the various ingredients, ultimately achieving a therapeutic effect superior to that of a single prescription.

[0021] Furthermore, the extract B is prepared by enzymolysis of sour jujube seeds, tangerine peel, yam and white tea using a mixed enzyme.

[0022] Furthermore, the weight ratio of the sour jujube seeds, tangerine peel, yam and white tea is (16-18):10:(2-5):(11-13).

[0023] Furthermore, the enzyme mixture includes ficin and bromelain.

[0024] Furthermore, the preparation method of the extract B comprises the following steps:

[0025] (1) Mixing sour jujube seeds, dried tangerine peel, Chinese yam and white tea, and grinding to less than 60 mesh to obtain powder B;

[0026] (2) Powder B and water are mixed evenly in a weight ratio of 1:(6-8) to obtain an enzymatic hydrolysis material; mixed enzyme is added to the enzymatic hydrolysis material, and enzymolysis is carried out at 40-45°C for 7-9 hours. After sterilization, an enzymatic hydrolysis product is obtained;

[0027] (3) The enzymatic hydrolysis product was filtered through a 0.22 μm filter membrane, and the filtrate was spray-dried to obtain extract B.

[0028] Furthermore, the dosage of the ficin is 100-110 U / mL of the enzymatic hydrolysis material; the dosage of the bromelain is 100-110 U / mL of the enzymatic hydrolysis material.

[0029] Certain components of deer blood extract may reduce inflammatory responses by regulating the immune system. They can reduce the levels of pro-inflammatory factors such as TNF-α and IL-6 by scavenging free radicals and inhibiting the release of inflammatory mediators, thereby alleviating the symptoms of inflammatory anemia. Deer blood extract, extract A, and extract B can show significant synergistic effects in the treatment of inflammatory anemia. This synergistic effect can inhibit the secretion of pro-inflammatory factors TNF-α and IL-6, and can also improve patients' anemia symptoms from multiple levels, including promoting iron absorption, enhancing hematopoietic function, and regulating immune balance.

[0030] The present invention provides a method for preparing a traditional Chinese medicine composition for nourishing blood and calming the mind, comprising the following steps:

[0031] (1) Mix polyvinyl pyrrolidone and water to prepare a bonding solution with a concentration of 13-15 wt%, heat it to 40-45°C and maintain it for later use;

[0032] (2) Mixing the deer blood extract, extract A, extract B, and starch, spraying the mixture into the binding solution, and wet granulating the mixture;

[0033] (3) Dry the granules at 40-45°C, shape the granules, and finally add magnesium stearate and mix them evenly to obtain a Chinese medicine composition for nourishing blood and calming the mind.

[0034] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0035] (1) The present invention provides a Chinese herbal medicine composition for nourishing blood and calming the mind. It is scientifically formulated using a variety of Chinese medicinal materials that have the effects of nourishing yin and blood, nourishing the heart and calming the mind. It can effectively improve symptoms such as palpitations, insomnia, frequent dreams and easy awakening, and mental fatigue caused by insufficient heart blood and malnutrition of the heart and mind. This preparation is formulated into a composition dosage form under the guidance of traditional Chinese medicine theory and combined with modern pharmaceutical technology, which better meets the modern population's demand for efficient, safe, and convenient health products.

[0036] (2) The blood-nourishing and mind-calming Chinese medicine composition provided by the present invention has positive significance for promoting the application of traditional Chinese medicine in the field of sleep. In terms of pharmacological mechanism, the Chinese medicine composition regulates the function of the central nervous system and improves the balance of neurotransmitters through the synergistic action of multiple targets and multiple pathways, and has good sedative, anti-anxiety and sleep-promoting effects. Secondly, in clinical application, it can be widely used to treat mild to moderate insomnia and anxiety caused by factors such as high work pressure, fast pace of life, and qi and blood imbalance, and has promotion value. Thirdly, in terms of dosage form innovation, the traditional decoction is transformed into a modern Chinese medicine preparation that is standardized, has a clear dosage, and is easy to carry and take, which helps to improve the scientific and technological content and market competitiveness of Chinese medicine products. DETAILED DESCRIPTION

[0037] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0038] The raw materials used in the following examples of the present invention are all commercially available commodities:

[0039] Polyvinyl pyrrolidone was purchased from Xi'an Tianzheng Pharmaceutical Excipients Co., Ltd., polyvinyl pyrrolidone K30.

[0040] Deer blood extract, i.e., deer blood freeze-dried powder, was purchased from Shaanxi Fangxin Biotechnology Co., Ltd.

[0041] Bacillus subtilis, strain number MCCC1A11421, was purchased from China Marine Microbial Culture Collection Center.

[0042] Bacillus fusiformis, strain number CICC23677, was purchased from the China Industrial Microbiology Culture Collection Center.

[0043] Lactobacillus paracasei, strain number CICC6107, was purchased from the China Industrial Microbiology Culture Collection Center.

[0044] Bromelain, activity 1200 u / mg. Shanghai Yuanye Biotechnology Co., Ltd.

[0045] Ficin, activity 100 u / mg. Shanghai Yuanye Biotechnology Co., Ltd.

[0046] Example 1

[0047] This embodiment provides a traditional Chinese medicine composition for nourishing blood and calming the mind, comprising the following components in parts by weight: 150 parts of deer blood extract, 55 parts of extract A, 84 parts of extract B, 125 parts of starch, 12 parts of polyvinyl pyrrolidone, and 1.5 parts of magnesium stearate;

[0048] The preparation method of the extract A comprises the following steps:

[0049] (1) Mix Cuscuta seeds, white peony root, Chinese angelica root and wolfberry in a weight ratio of 6:10:4:13, and grind to less than 60 mesh to obtain powder A;

[0050] (2) Powder A and water in a weight ratio of 1:7 were mixed evenly to obtain a fermentation material; Bacillus subtilis was first added to the fermentation material, and aerobic fermentation was carried out at 37°C for 21 hours. After sterilization, Clostridium was added, and anaerobically fermentation was carried out at 37°C for 27 hours. After sterilization, Lactobacillus paracasei was added, and anaerobically fermentation was carried out at 37°C for 28 hours. After sterilization, a fermentation product was obtained; the amount of Bacillus subtilis was 10 7 CFU / mL fermentation material; the amount of Clostridium is 10 8 CFU / mL fermentation material; the dosage of Lactobacillus paracasei is 10 9 CFU / mL fermentation feed.

[0051] (3) The fermentation product was filtered through a 0.22 μm filter membrane, and the filtrate was spray-dried to obtain extract A.

[0052] The preparation method of the extract B comprises the following steps:

[0053] (1) Mixing jujube seeds, dried tangerine peel, Chinese yam and white tea in a weight ratio of 17:10:3:12, and grinding the mixture to a size smaller than 60 mesh to obtain powder B;

[0054] (2) Powder B and water were mixed uniformly in a weight ratio of 1:7 to obtain an enzymatic hydrolysis material; an enzyme mixture was added to the enzymatic hydrolysis material, and the mixture was enzymatically hydrolyzed at 42°C for 8 hours. After sterilization, an enzymatic hydrolysis product was obtained; the enzyme mixture included ficin and bromelain. The amount of ficin was 105 U / mL of the enzymatic hydrolysis material; the amount of bromelain was 105 U / mL of the enzymatic hydrolysis material.

[0055] (3) The enzymatic hydrolysis product was filtered through a 0.22 μm filter membrane, and the filtrate was spray-dried to obtain extract B.

[0056] The preparation method of the traditional Chinese medicine composition for nourishing blood and calming the mind comprises the following steps:

[0057] (1) Mix polyvinyl pyrrolidone K30 and water to prepare a bonding solution with a concentration of 14 wt%, heat it to 42 ° C and maintain it for later use;

[0058] (2) Mixing the deer blood extract, extract A, extract B, and starch, spraying the mixture into the binding solution, and wet granulating the mixture;

[0059] (3) The granules are dried at 42°C and sized, and finally magnesium stearate is added and mixed evenly to obtain a Chinese medicine composition for nourishing blood and calming the mind.

[0060] Example 2

[0061] This embodiment provides a Chinese medicine composition for nourishing blood and calming the mind, comprising the following components in parts by weight: 150 parts of deer blood extract, 50 parts of extract A, 90 parts of extract B, 120 parts of starch, 15 parts of polyvinyl pyrrolidone, and 2 parts of magnesium stearate;

[0062] The preparation method of the extract A comprises the following steps:

[0063] (1) Mix Cuscuta seeds, white peony root, Chinese angelica root and wolfberry in a weight ratio of 5:10:3:14, and grind to less than 60 mesh to obtain powder A;

[0064] (2) Powder A and water in a weight ratio of 1:8 were mixed evenly to obtain a fermentation material; Bacillus subtilis was first added to the fermentation material, and aerobic fermentation was carried out at 35°C for 22 hours. After sterilization, Clostridium was added, and anaerobically fermentation was carried out at 35°C for 26 hours. After sterilization, Lactobacillus paracasei was added, and anaerobically fermentation was carried out at 35°C for 29 hours. After sterilization, a fermentation product was obtained; the amount of Bacillus subtilis was 10 7 CFU / mL fermentation material; the amount of Clostridium is 10 7 CFU / mL fermentation material; the dosage of Lactobacillus paracasei is 10 8 CFU / mL fermentation feed.

[0065] (3) The fermentation product was filtered through a 0.22 μm filter membrane, and the filtrate was spray-dried to obtain extract A.

[0066] The preparation method of the extract B comprises the following steps:

[0067] (1) Mix jujube, dried tangerine peel, Chinese yam and white tea in a weight ratio of 17:10:3:12, and grind to less than 60 mesh to obtain powder B;

[0068] (2) Powder B and water were mixed uniformly in a weight ratio of 1:8 to obtain an enzymatic hydrolysis material; an enzyme mixture was added to the enzymatic hydrolysis material, and the mixture was enzymatically hydrolyzed at 40°C for 9 hours. After sterilization, an enzymatic hydrolysis product was obtained; the enzyme mixture included ficin and bromelain. The amount of ficin was 100 U / mL of the enzymatic hydrolysis material; the amount of bromelain was 110 U / mL of the enzymatic hydrolysis material.

[0069] (3) The enzymatic hydrolysis product was filtered through a 0.22 μm filter membrane, and the filtrate was spray-dried to obtain extract B.

[0070] The preparation method of the traditional Chinese medicine composition for nourishing blood and calming the mind comprises the following steps:

[0071] (1) Mix polyvinyl pyrrolidone K30 and water to prepare a bonding solution with a concentration of 13 wt%, heat it to 45 ° C and maintain it for later use;

[0072] (2) Mixing the deer blood extract, extract A, extract B, and starch, spraying the mixture into the binding solution, and wet granulating the mixture;

[0073] (3) Drying and shaping the granules at 40°C, and finally adding magnesium stearate and mixing evenly to obtain a Chinese medicine composition for nourishing blood and calming the mind.

[0074] Comparative Example 1

[0075] The difference between this comparative example and Example 1 is that: 55 parts of extract A and 84 parts of extract B are replaced by: 84 parts of extract A and 55 parts of extract B.

[0076] Comparative Example 2

[0077] The difference between this comparative example and Example 1 is that the weight ratio of Cuscuta seeds, white peony root, Chinese angelica and wolfberry is 2:10:1:5.

[0078] Comparative Example 3

[0079] The difference between this comparative example and Example 1 is that wolfberry is replaced by cornus officinalis.

[0080] Comparative Example 4

[0081] The difference between this comparative example and Example 1 is that the weight ratio of sour jujube kernel, tangerine peel, yam and white tea is 5:10:7:3.

[0082] Comparative Example 5

[0083] The difference between this comparative example and Example 1 is that white tea is replaced by Dangshen.

[0084] Comparative Example 6

[0085] The difference between this comparative example and Example 1 is that the order of fermentation of the bacteria used in the preparation of the extract A is different.

[0086] The preparation method of the extract A comprises the following steps:

[0087] (1) Mix the Semen Cuscutae, Radix Paeoniae Alba, Angelica sinensis and Lycium barbarum in a weight ratio of 6:10:4:13, and then crush them to less than 60 mesh to obtain a powder A;

[0088] (2) Mix the powder A and water in a weight ratio of 1:7 to obtain a fermentation material; first add Lactobacillus casei to the fermentation material, and then anaerobically ferment at 37°C for 28h; after sterilization, continue to add Clostridium sporosphaeroides, and then anaerobically ferment at 37°C for 27h; after sterilization, continue to add Bacillus subtilis, and then aerobically ferment at 37°C for 21h; after sterilization, obtain a fermentation product; the amount of Bacillus subtilis is 10 7 CFU / mL of the fermentation material; the amount of Clostridium sporosphaeroides is 10 8 CFU / mL of the fermentation material; and the amount of Lactobacillus casei is 10 9 CFU / mL of the fermentation material.

[0089] (3) Filter the fermentation product through a 0.22μm filter membrane, and then spray dry the filtrate to obtain the extract A.

[0090] Performance test

[0091] 1. The compositions prepared in Examples 1-2 and Comparative Examples 1-6 are tested for their effects on improving the hemoglobin content and body weight of rats with iron deficiency anemia according to the performance test method in the patent CN201611227018.0 A Qiwei Weixi Tablet and a preparation method thereof.

[0092] SPF grade weaned female SD rats are used, 10 rats in each group, which are blank group, control group, and compositions prepared in Examples 1-2 and Comparative Examples 1-6. The control group is a deer blood extract, and the intragastrical dose is 100mg / kg·BW; the intragastrical dose of the drug granules prepared in Examples 1-2 and Comparative Examples 1-6 is 100mg / kg·BW. The blank group is given deionized water, and the intragastrical amount is 1mL / 100g·BW. Each group is continuously intragastrically administered for 30d. The tail blood of the rats is taken to detect the hemoglobin concentration.

[0093] The results are shown in Table 1.

[0094] Table 1 Test results of the effects of iron deficiency anemia Hemoglobin content before the test g / L Hemoglobin content after the test g / L Blank group 80.7±11.5 77.1±10.7 control group 79.2±12.4 86.7±11.3 Example 1 81.6±11.8 97.2±10.5 Example 2 81.4±10.9 96.6±12.2 Comparative Example 1 80.9±11.0 89.8±12.4 Comparative Example 2 79.3±11.1 92.5±11.0 Comparative Example 3 79.5±10.7 91.4±10.9 Comparative Example 4 81.7±12.6 90.9±12.1 Comparative Example 5 80.1±11.3 92.0±11.8 Comparative Example 6 80.8±12.2 93.3±12.7

[0095] From the above performance test results, it can be seen that the Chinese medicine compositions of Examples 1 and 2 have excellent effects on improving iron deficiency anemia.

[0096] In Comparative Example 1, the amount of extract A and extract B was changed, and it can be seen that the improvement effect on iron deficiency anemia decreased. This shows that extract A and extract B have a synergistic effect under a specific ratio and can jointly improve the effect of iron deficiency anemia.

[0097] In Comparative Example 2, the ratio of Cuscuta australis, white peony root, Chinese angelica root and wolfberry was changed, and it can be seen that the improvement effect on iron deficiency anemia was reduced, indicating that the extract A prepared by combining these three raw materials in a specific ratio has the best effect in the system.

[0098] In Comparative Example 3, wolfberry was replaced with cornus officinalis. It can be seen that the improvement effect on iron deficiency anemia was reduced, indicating that the selection of the raw materials of extract A was not random, but had a synergistic effect with each other.

[0099] In Comparative Example 4, the ratio of Chinese jujube seeds, dried tangerine peel, Chinese yam and white tea was changed, and it can be seen that the improvement effect on iron deficiency anemia was reduced, indicating that the extract B prepared by these four raw materials in a specific ratio has the best effect in the system.

[0100] In Comparative Example 5, white tea was replaced with Codonopsis pilosula. It can be seen that the improvement effect on iron deficiency anemia was reduced, indicating that the selection of the raw materials of Extract B was not random, but had a synergistic effect with each other.

[0101] In Comparative Example 6, the fermentation order of the bacteria used to prepare Extract A was different, and it can be seen that the improvement effect on iron deficiency anemia was reduced, indicating that when preparing Extract A, if the order of using bacteria was changed, the fermentation products of the bacteria would be different, and the active components in Extract A would change.

[0102] In the control group, only the deer blood extract was used, and the improvement effect on iron deficiency anemia was lower than that in Example 1-2, indicating that the extract A, extract B and deer blood extract of the present invention have a synergistic effect in treating iron deficiency anemia.

[0103] 2. Trials for the treatment of anemia of inflammation

[0104] Experimental animals: 55 male SD rats, 150-155 g, 7 weeks old, 5 rats per group.

[0105] A model was established in 50 rats using a subcutaneous injection of 0.15 mL of Freund's complete adjuvant (10 mg / mL) containing Mycobacterium tuberculosis. Five of these rats served as the model group and were gavaged daily with 1 mL of deionized water. The remaining 45 rats were divided into nine groups and orally administered with the deer blood extract prepared according to the present invention, the pharmaceutical granules of Examples 1-2, and Comparative Examples 1-6, at a dose of 2.16 g / kg body weight daily.

[0106] Five rats were injected subcutaneously with 0.15 mL of normal saline into their toes and served as a control group.

[0107] Each group received oral administration for 30 days.

[0108] The results are shown in Table 2.

[0109] Table 2 Test results of inflammatory anemia TNF-α IL-6 control group 250.3±1.2 125.8±2.1 Model Group 422.6±6.9 156.3±1.4 Example 1 271.2±5.7 134.6±3.3 Example 2 273.7±4.1 136.4±2.8 Comparative Example 1 325.1±6.2 145.1±1.5 Comparative Example 2 316.4±5.6 142.5±1.7 Comparative Example 3 320.5±5.3 141.2±2.2 Comparative Example 4 323.8±6.8 143.9±3.9 Comparative Example 5 319.0±4.4 142.7±3.6 Comparative Example 6 301.9±4.5 140.3±1.4 Deer blood extract 358.7±5.0 149.1±1.5

[0110] From the above performance test results, it can be seen that the Chinese medicine composition of Examples 1-2 can significantly inhibit the secretion of pro-inflammatory factors TNF-α and IL-6 in rat serum.

[0111] In Comparative Example 1, the amount of extract A and extract B was changed, and it can be seen that the effect of inhibiting the secretion of pro-inflammatory factors TNF-α and IL-6 in rat serum was reduced. This shows that extract A and extract B have a synergistic effect at a specific ratio, jointly inhibiting the secretion of pro-inflammatory factors TNF-α and IL-6 in rat serum.

[0112] In Comparative Example 2, the ratio of Cuscuta australis, white peony root, Chinese angelica and wolfberry was changed, and it can be seen that the effect of inhibiting the secretion of pro-inflammatory factors TNF-α and IL-6 in rat serum was reduced, indicating that the extract A prepared by these three raw materials in a specific ratio exerted the best inhibitory effect in the system.

[0113] In Comparative Example 3, wolfberry was replaced with cornus officinalis. It can be seen that the effect of inhibiting the secretion of pro-inflammatory factors TNF-α and IL-6 in rat serum was reduced, indicating that the selection of the raw materials of extract A was not random, but had a synergistic effect with each other.

[0114] In Comparative Example 4, the ratio of Chinese jujube kernel, tangerine peel, yam and white tea was changed, and it can be seen that the effect of inhibiting the secretion of pro-inflammatory factors TNF-α and IL-6 in rat serum was reduced, indicating that the extract B prepared by these four raw materials in a specific ratio exerted the best inhibitory effect in the system.

[0115] In Comparative Example 5, white tea was replaced with Codonopsis pilosula. It can be seen that the effect of inhibiting the secretion of pro-inflammatory factors TNF-α and IL-6 in rat serum was reduced, indicating that the selection of the raw materials of Extract B was not random, but had a synergistic effect with each other.

[0116] The fermentation sequence of the bacteria used in the preparation of the extract A in Comparative Example 6 was different, and it can be seen that the effect on iron deficiency anemia was improved and reduced, indicating that when preparing the extract A, if the use sequence of the bacteria is changed, the bacteria fermentation product is different, and the active components in the extract A are changed.

[0117] The effect of using only deer blood extract on iron deficiency anemia was significantly lower than that of Example 1-2, indicating that the extract A, the extract B and the deer blood extract of the present application have a synergistic effect.

[0118] The above is a preferred embodiment of the present application, it should be noted that for those skilled in the art, without departing from the principles of the present application, a number of improvements and refinements can also be made, which should be considered as the protection scope of the present application.

Claims

1. A Chinese medicine composition for nourishing blood and calming the mind, characterized in that: The traditional Chinese medicine composition comprises the following components in parts by weight: 150 parts of deer blood extract, 50-60 parts of extract A, 80-90 parts of extract B, 120-130 parts of starch, 10-15 parts of polyvinyl pyrrolidone, and 1-2 parts of magnesium stearate; The extract A is prepared by fermenting Cuscuta seeds, white peony root, Chinese angelica root and wolfberry; The extract B is prepared by enzymatic hydrolysis of jujube seeds, dried tangerine peel, Chinese yam and white tea.

2. The Chinese medicine composition for nourishing blood and calming the mind according to claim 1, characterized in that The extract A is prepared by fermenting Cuscuta seeds, white peony root, Chinese angelica root and wolfberry in sequence using Bacillus subtilis, Clostridium and Lactobacillus paracasei.

3. The Chinese medicine composition for nourishing blood and calming the mind according to claim 2, characterized in that The weight ratio of the dodder seed, white peony root, angelica root and wolfberry is (5-8):10:(3~5):(12~14).

4. The Chinese medicine composition for nourishing blood and calming the mind according to claim 3, characterized in that The preparation method of the extract A comprises the following steps: (1) Mix Cuscuta seeds, white peony root, Chinese angelica root, and wolfberry, and grind them to obtain powder A; (2) Powder A and water are mixed evenly to obtain a fermentation material; Bacillus subtilis is first added to the fermentation material, and aerobic fermentation is carried out at 35-40°C for 20-22 hours. After sterilization, Clostridium is continuously added, and anaerobically fermentation is carried out at 35-40°C for 26-28 hours. After sterilization, Lactobacillus paracasei is continuously added, and anaerobically fermentation is carried out at 35-40°C for 27-29 hours. After sterilization, a fermentation product is obtained; (3) The fermentation product is filtered, and the filtrate is spray-dried to obtain extract A.

5. The Chinese medicine composition for nourishing blood and calming the mind according to claim 4, characterized in that: The dosage of Bacillus subtilis is 10 7 ~10 8 CFU / mL fermentation material; the amount of Clostridium is 10 7 ~10 8 CFU / mL fermentation material; the dosage of Lactobacillus paracasei is 10 8 ~10 9 CFU / mL fermentation feed.

6. The Chinese medicine composition for nourishing blood and calming the mind according to claim 1, characterized in that The extract B is prepared by enzymolysis of wild jujube seeds, dried tangerine peel, Chinese yam and white tea using mixed enzymes.

7. The Chinese medicine composition for nourishing blood and calming the mind according to claim 6, characterized in that The weight ratio of the sour jujube seeds, tangerine peel, yam and white tea is (16-18):10:(2-5):(11-13).

8. The Chinese medicine composition for nourishing blood and calming the mind according to claim 7, characterized in that: The enzyme mixture includes ficin and bromelain.

9. The Chinese medicine composition for nourishing blood and calming the mind according to claim 8, characterized in that The preparation method of the extract B comprises the following steps: (1) Mixing the sour jujube seeds, dried tangerine peel, Chinese yam and white tea, and grinding them to obtain powder B; (2) Powder B and water are mixed evenly to obtain an enzymatic hydrolysis material; mixed enzyme is added to the enzymatic hydrolysis material, and enzymatic hydrolysis is carried out at 40-45°C for 7-9 hours. After sterilization, an enzymatic hydrolysis product is obtained; (3) The enzymatic hydrolysis product is filtered and the filtrate is spray-dried to obtain extract B.

10. A method for preparing the blood-nourishing and mind-soothing Chinese medicinal composition according to any one of claims 1 to 9, characterized in that: The following steps are involved: (1) Mix polyvinyl pyrrolidone and water to prepare a bonding solution with a concentration of 13-15 wt%, heat it to 40-45°C and maintain it for later use; (2) Mixing the deer blood extract, extract A, extract B, and starch, spraying the mixture into the binding solution, and wet granulating the mixture; (3) Dry the granules at 40-45°C, shape the granules, and finally add magnesium stearate and mix them evenly to obtain a Chinese medicine composition for nourishing blood and calming the mind.