Fermentation equipment convenient to incline and used for cell culture

Abstract

The invention discloses fermentation equipment convenient to incline for cell culture, and relates to the technical field of cell culture fermentation equipment. Comprising a frame, the outer side of the frame is fixedly connected with a motor, the output end of the motor penetrates through the frame, the inner wall of the frame is rotationally connected with a rotating shaft, the two ends of the rotating shaft are rotationally connected with the two side edges of the frame respectively, and the outer side, close to the frame, of the rotating shaft is fixedly connected with a gear; and the rotating piece is meshed with an outer side gear of the gear. According to the fermentation equipment convenient to incline and used for cell culture, the fermentation tank is driven to rotate through a connecting piece by a round rod, the fermentation tank swings back and forth along with forward and reverse rotation of a motor, local accumulation of nutrient substances or metabolites in a culture solution in the fermentation tank can be avoided through periodic swinging, uniform distribution is achieved through slow mixing, and the fermentation efficiency is improved. A stable culture environment is maintained, the cell logarithmic phase is prolonged, and the product yield is improved.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture and fermentation equipment, in particular to a cell culture and fermentation equipment which is easy to tilt. Background Art

[0002] The rapid development of bioengineering technology has enabled genetically engineered drugs to enter clinical use. Genetic engineering products such as GMCS, GCSF, TPO, and EGF have not only brought enormous social benefits but also considerable economic benefits to pharmaceutical companies. Fermentation is a key component of bioengineering, and fermentation equipment is a key component of bioengineering equipment. The goal of fermentation equipment research is to provide optimal growth and reproduction conditions for engineered bacteria or cells, specifically, to provide optimal external conditions such as temperature, pH value, stirring speed, and dissolved oxygen content, so that after growth and reproduction, the engineered bacteria or cells can produce the maximum amount of various biochemical substances needed by humans in the form of inclusion bodies or supernatant.

[0003] Existing fermentation equipment does not mix and stir the cells and culture liquid in the fermentation tank evenly enough, and the stirring rod will cause vortexes to form in the center during the stirring process of the liquid, causing microorganisms to accumulate in the center, affecting the content of microorganisms in the unit liquid, resulting in poor fermentation effect of the fermentation tank, long fermentation time, and affecting work efficiency. Summary of the Invention

[0004] To achieve the above objectives, the present invention is implemented through the following technical solutions: A fermentation device for cell culture that is easy to tilt, comprising a fermentation tank and a connector fixedly installed on the outside;

[0005] A bracket, wherein the bracket is fixedly connected to the connecting piece;

[0006] Among them, the bracket includes a frame, the frame is a gantry design, the outer side of the frame is fixedly connected to a motor, the output end of the motor passes through the frame, the inner wall of the frame is rotatably connected to a rotating shaft, the two ends of the rotating shaft are respectively rotatably connected to the two side edges of the frame, the rotating shaft is fixedly connected to a gear near the outer side of the frame, the inner wall of the frame is rotatably connected to a rotating part, the rotating part is meshed with the outer gear of the gear, and the rotating part is clamped with the connecting part.

[0007] Preferably, a fixing seat is fixedly connected to the bottom of the frame, and there are two fixing seats. The two fixing seats are symmetrically arranged with the rotating shaft as the center. The fermenter is installed on the bracket through a connecting piece. The fermenter starts to work and the motor is connected to an external power supply. The motor drives the rotating shaft to rotate, so that the rotating shaft drives the gears at both ends to rotate. The rotation of the gears drives the gear rings that are meshed with the gears to rotate, and then the gear rings drive the middle rod and the circular ring to rotate, so that the circular ring drives the round rod to rotate. At this time, the round rod drives the fermenter to rotate through the connecting piece. With the forward and reverse rotation of the motor, the fermenter swings back and forth. The periodic swing can avoid local accumulation of nutrients or metabolites in the culture solution inside the fermenter, and achieve uniform distribution through slow mixing, maintain a stable culture environment, prolong the logarithmic growth period of cells, and improve the product yield. In addition, the periodic swing of the fermenter will make The culture medium inside the fermentation tank generates a wave-like flow, which makes the cells evenly dispersed in the culture medium, avoids precipitation or local aggregation, and maintains sufficient contact between the cells and nutrients. There are two gears, and the two gears are symmetrically arranged with the rotating shaft as the center. A connecting plate is fixedly connected to the middle of the outer side of the frame, and an arc plate is fixedly connected to the end of the connecting plate away from the frame. There are four connecting plates, and the four connecting plates are divided into two groups. The connecting plates of the two groups are symmetrically arranged on the two sides of the frame with the rotating shaft as the center. The two connecting plates in one group are symmetrically arranged with the motor as the center. One end of the arc plate is fixedly connected to one end of the connecting plate, and the end of the arc plate away from the connecting plate is fixedly connected to the side of the frame. The two arc plates and the two connecting plates on one side of the frame form a semicircle, and the axis of the semicircle coincides with the axis of the gear ring. A ring groove is provided on the side of the arc plate close to the rotating shaft.

[0008] Preferably, the rotating member includes a gear ring, the outer side of the gear ring is meshed with the outer side of the gear, the side of the gear ring away from the gear is fixedly connected to an intermediate rod, the intermediate rod is located in the middle of the gear ring, the end of the intermediate rod away from the gear ring is fixedly connected to a circular ring, the internal rotation of the frame is connected to a round rod, the round rod passes through the frame and extends to the inside of the frame, the outer side of the round rod is fixedly connected to the circular ring, the circular ring sleeve is arranged on the outer side of the round rod, the outer top of the round rod is fixedly connected to a protrusion, the protrusion is located on the side of the circular ring away from the frame, the end of the round rod is fixedly connected to a fixing plate, the side of the fixing plate close to the round rod is in contact with the outer side of the frame, and the gear ring is close to A cylinder is fixedly connected to one side of the arc plate, and the motor is connected to an external power supply to work. The motor drives the rotating shaft to rotate, so that the rotating shaft drives the outer gear to rotate, and the gear drives the gear ring to rotate. At the same time, the cylinder on the gear ring slides inside the ring groove. At this time, the ring groove provides a rigid guide for the swing of the gear ring and the fermentation tank, ensuring that the fermentation tank remains stable during movement and avoids shaking or offset. The cooperation between the cylinder and the ring groove can disperse mechanical stress, reduce the wear rate of moving parts, and extend the service life of the equipment. There are two cylinders, which are symmetrically arranged at both ends of the gear ring, and the cylinder is located inside the ring groove.

[0009] Preferably, the connecting piece includes a fixing ring, which is sleeved on the outside of the fermentation tank and fixedly connected to the fermentation tank. A fixing block is fixedly connected to the middle of the outside of the fixing ring. There are two fixing blocks, which are symmetrically arranged with the fixing ring as the center. The fixing block is located on the outside of the fixing ring close to the round rod. A square groove is provided at one end of the fixing block away from the fixing ring. The square groove passes through the fixing block and extends vertically downward. A contact plate is slidably connected to the inside of the square groove. When the fermentation tank is installed, the round rod moves along the opening of the square groove toward the inside of the square groove, so that the round rod contacts the inclined plate and produces The round rod is squeezed and compressed by the inclined plate, so that the round rod passes smoothly through the inclined plates symmetrically arranged on both sides of the square groove. Then, the end of the round rod away from the fixed plate is located inside the square groove on the fixed block, and the round rods on both sides are located inside the square grooves on both sides of the fixed ring respectively. The elastic performance of the spring plate is used to make the contact plate and the round rod in close contact. Due to long-term use, a wear gap will be generated between the round rod and the contact plate, which can be adaptively compensated by the elastic deformation of the spring plate to prevent the fermenter from loosening due to the expansion of the gap. At this time, the fermenter and the connector are clamped on the bracket through the round rod and the square groove, and the inside of the square groove A spring plate is provided at the top, and the two ends of the spring plate are fixedly connected to the contact plate and the inner wall of the square groove respectively. A groove is opened in the middle of the inner wall of the square groove, and the protrusion is located inside the groove. The fermentation tank and the connecting piece are clamped on the bracket, and the motor is connected to the external power supply to work. The motor drives the rotating shaft to rotate, so that the rotating shaft drives the outer gear to rotate, and the gear drives the gear ring to rotate. The gear ring drives the round rod to rotate through the middle rod and the circular ring, so that the round rod drives the fixed ring and the fixed block to rotate through the protrusion, thereby driving the fermentation tank to swing back and forth, and then the ball located on the outside of the tank body swings along with the swing of the tank body. The movement causes a slight collision between the tank body and the ball. The vibration generated by the collision on the outside of the tank body will be transmitted to the tank wall, causing the sticky material attached to the inner wall of the tank body to fall off, reducing the frequency of cleaning. The inner wall of the square groove is fixedly connected with an inclined plate. The inclined plate is elastic. There are two inclined plates. The inclined plates are located at the end of the square groove away from the groove, and the inclined plates are symmetrically arranged inside the square groove. The outside of the fixed ring is fixedly connected with an extension block. There are two extension blocks. The extension blocks and the fixed blocks are alternately arranged. The bottom of the extension block is fixedly connected with a spring, and the end of the spring away from the extension block is fixedly connected with the ball.

[0010] Preferably, the fermentation tank includes a tank body, which is fixedly connected to a fixed ring, a feed port is provided on the top of the tank body, an exhaust port is fixedly connected to the top of the tank body away from the feed port, an electric motor is fixedly connected to the middle of the top of the tank body, the output end of the motor passes through the tank body and extends to the interior of the tank body, a stirring member is provided inside the tank body, a discharge port is provided on the outer bottom of the tank body, the stirring member is fixedly connected to the motor, and a ring block is fixedly connected to the middle of the inner wall of the tank body.

[0011] Preferably, the stirring member includes a rotating shaft, which is fixedly connected to the output end of the motor, a middle block is fixedly connected to the middle of the outer side of the rotating shaft, a fixed rod is fixedly connected to the outer side of the middle block, a slide groove is provided on the end face of the fixed rod away from the middle block, the ring block is located inside the slide groove, there are three fixed rods, and the three fixed rods are evenly distributed on the outer side of the middle block, the culture medium and cells are added to the interior of the tank through the feed port, the motor is connected to the external power supply to work, the motor drives the rotating shaft to rotate, the rotating shaft drives the middle block in the middle and the sliders at both ends to rotate, so that the middle block and the slider respectively drive the fixed rod and the extension rod to rotate, stirring the interior of the tank in the upper, middle and lower directions, and the extension rod and the fixed rod drive The rotation of the spiral plate and the arc plate causes the spiral plate to push the material along the axial direction of the tank, forming an axial laminar flow, thereby pushing the bottom material to circulate to the top. At this time, the arc plate generates a radial thrust opposite to the main spiral, forcing the material to diffuse in the direction away from the flow, forming radial turbulence, ensuring that the cells in each area can fully contact nutrients, reducing cell death caused by nutrient deficiency or accumulation of metabolic waste. At the same time, the interaction and offset of the two opposite spiral flows can reduce the shear force generated by stirring. In a low shear force environment, it can effectively improve cell survival rate, maintain the normal morphology and function of cells, and reduce cell apoptosis or metabolic abnormalities caused by mechanical damage. The outer rotation of the shaft is connected with a slider. There are two sliders, and the two sliders are symmetrically arranged with the middle block as the center. The outer side of the slider is fixedly connected with an extension rod. There are multiple extension rods, and the multiple extension rods are evenly distributed on the outer side of the slider. A spiral plate is arranged on the outer side of the rotating shaft. There are multiple spiral plates, and the multiple spiral plates are divided into two groups. The two groups of spiral plates are symmetrically arranged with the middle block as the center. The two ends of the spiral plate of one group are respectively fixedly connected to the top of the fixed rod and the extension rod located above the middle block, and the two ends of the spiral plate of the other group are respectively fixedly connected to the bottom of the fixed rod and the extension rod located below the middle block. The outer side of the spiral plate is fixedly connected with an arc plate, and the arc plate is spirally arranged, and the spiral direction of the arc plate is opposite to that of the spiral plate. The bottom of the extension rod is fixedly connected to a defoaming needle, and the motor is connected to an external power supply to work. The motor drives the rotating shaft to rotate. At this time, the rotating shaft drives the slider to rotate through the fixed column located inside the card slot, so that the slider drives the extension rod and the defoaming needle to rotate, so that the centrifugal force and tangential speed generated by the rotation of the defoaming needle can exert shear force on the foam, causing the foam to burst, and the gas generated during the fermentation process is discharged from the exhaust port on the top of the tank body. The defoaming needle is located on the extension rod above the middle block, and a rotating plate is fixedly connected to the outer side of the end of the rotating shaft. There are multiple rotating plates, and the multiple rotating plates are symmetrically arranged at both ends of the rotating shaft. The inside of the slider is fixedly connected to a fixed column, and a card slot is provided on the outside of the rotating shaft, and the fixed column is located inside the card slot.

[0012] The present invention provides a cell culture fermentation device that is easy to tilt and has the following beneficial effects:

[0013] 1. This easy-to-tilt cell culture fermentation equipment uses a round rod through a connecting piece to drive the fermenter to rotate. As the motor rotates forward and reverse, the fermenter swings back and forth. The periodic swing can avoid local accumulation of nutrients or metabolites in the culture solution inside the fermenter, achieve uniform distribution through slow mixing, maintain a stable culture environment, extend the logarithmic growth period of cells, and improve product yield.

[0014] Second, the tiltable cell culture fermentation equipment provides a rigid guide for the swing of the gear ring and the fermentation tank through the ring groove, ensuring that the fermentation tank remains stable during movement and avoids shaking or deviation. The fit between the cylinder and the ring groove can disperse mechanical stress, reduce the wear rate of moving parts, and extend the service life of the equipment.

[0015] Third, the cell culture fermentation equipment that is easy to tilt utilizes the elastic properties of the spring plate to ensure close contact between the contact plate and the round rod. Due to the wear gap between the round rod and the contact plate during long-term use, the elastic deformation of the spring plate can adaptively compensate for the wear gap, thereby preventing the fermenter from loosening due to the expansion of the gap.

[0016] Fourth, this easy-to-tilt cell culture fermentation equipment can reduce the shear force generated by stirring through the interaction and offset of two opposite spiral flows. In a low-shear environment, it can effectively improve cell survival rate, maintain the normal morphology and function of cells, and reduce cell apoptosis or metabolic abnormalities caused by mechanical damage. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic structural diagram of the present invention as a whole;

[0018] Figure 2 Schematic diagram of the structure of the bracket of the present invention;

[0019] Figure 3 It is a structural schematic diagram of the rotating part of the present invention;

[0020] Figure 4 Schematic diagram of the structure of the connecting piece of the present invention;

[0021] Figure 5 It is a schematic structural diagram of a cross-section of the connecting piece of the present invention;

[0022] Figure 6 It is a structural schematic diagram of a cross-sectional view of a fermentation tank of the present invention;

[0023] Figure 7 Schematic diagram of the structure of the stirring element of the present invention;

[0024] Figure 8 It is a schematic structural diagram of a part of the stirring element of the present invention.

[0025] In the figure: 1. bracket; 11. frame; 12. connecting plate; 13. arc plate; 14. fixing seat; 15. ring groove; 16. motor; 17. rotating shaft; 18. gear; 19. rotating member; 191. gear ring; 192. intermediate rod; 193. circular ring; 194. circular rod; 195. protrusion; 196. fixing plate; 197. cylinder; 2. connecting member; 21. fixing ring; 22. fixing block; 23. square groove; 24. extension block; 25. spring; 26. ball; 27. groove ; 28. Inclined plate; 29. ​​Contact plate; 210. Spring plate; 3. Fermentation tank; 31. Tank body; 32. Feed port; 33. Exhaust port; 34. Motor; 35. Ring block; 36. Stirring element; 361. Rotating shaft; 362. Intermediate block; 363. Fixed rod; 364. Slide; 365. Slider; 366. Extension rod; 367. Spiral plate; 368. Arc plate; 369. Defoaming needle; 3610. Rotating plate; 3611. Fixed column; 3612. Slot; 37. Discharge port. DETAILED DESCRIPTION

[0026] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0027] The first embodiment, as Figures 1 to 3 As shown, the present invention provides a technical solution: a fermentation device for cell culture that is easy to tilt, comprising a fermentation tank 3 and a connector 2 fixedly mounted on the outside;

[0028] Bracket 1, bracket 1 is fixedly connected to connecting member 2;

[0029] Among them, the bracket 1 includes a frame 11, which is a gantry design. The frame 11 is composed of a top plate and two side edges. The outer side of the frame 11 is fixedly connected to a motor 16, and the output end of the motor 16 passes through the frame 11. The inner wall of the frame 11 is rotatably connected to a rotating shaft 17, and the two ends of the rotating shaft 17 are respectively rotatably connected to the two side edges of the frame 11. The rotating shaft 17 is fixedly connected to a gear 18 near the outer side of the frame 11, and the inner wall of the frame 11 is rotatably connected to a rotating member 19. The rotating member 19 is engaged with the outer gear of the gear 18, and the rotating member 19 is clamped with the connecting member 2.

[0030] The bottom of the frame 11 is fixedly connected with a fixing seat 14. There are two fixing seats 14. The two fixing seats 14 are symmetrically arranged with the rotating shaft 17 as the center. The fermenter 3 is installed on the bracket 1 through the connecting piece 2. The fermenter 3 starts to work. At the same time, the motor 16 is connected to the external power supply to work. The motor 16 drives the rotating shaft 17 to rotate, so that the rotating shaft 17 drives the gears 18 at both ends to rotate. The rotation of the gear 18 drives the gear ring 191 meshing with the gear 18 to rotate, and then the gear ring 191 drives the middle rod 192 and the ring 193 to rotate, so that the ring 193 drives the round rod 194 to rotate. At this time, the round rod 194 drives the fermenter 3 to rotate through the connecting piece 2. With the forward and reverse rotation of the motor 16, the fermenter 3 swings back and forth. The periodic swing can avoid local accumulation of nutrients or metabolites in the culture solution inside the fermenter 3, and achieves uniform distribution through slow mixing, maintains a stable culture environment, prolongs the logarithmic growth period of cells, and improves the product yield. The periodic swing of the fermenter 3 will The culture medium inside the fermentation tank 3 produces a wave-like flow, thereby causing the cells to be evenly dispersed in the culture medium, avoiding precipitation or local aggregation, and maintaining full contact between the cells and nutrients. There are two gears 18, and the two gears 18 are symmetrically arranged with the rotating shaft 17 as the center. A connecting plate 12 is fixedly connected to the middle of the outer side of the frame 11. The connecting plate 12 is located on the side of the frame 11, and the end of the connecting plate 12 away from the frame 11 is fixedly connected to the arc plate 13. There are four connecting plates 12, and the four connecting plates 12 are divided into two groups. The connecting plates 12 of the two groups are symmetrically arranged on the two sides of the frame 11 with the rotating shaft 17 as the center. The two connecting plates 12 in one group are symmetrically arranged with the motor 16 as the center. The end of the arc plate 13 away from the connecting plate 12 is fixedly connected to the side of the frame 11. The two arc plates 13 and the two connecting plates 12 located on one side of the frame 11 form a semicircle, and the axis of the semicircle coincides with the axis of the gear ring 191. A ring groove 15 is provided on the side of the arc plate 13 close to the rotating shaft 17.

[0031] The rotating member 19 includes a gear ring 191, the outer side of the gear ring 191 is meshed with the outer side of the gear 18, the side of the gear ring 191 away from the gear 18 is fixedly connected to an intermediate rod 192, the intermediate rod 192 is located in the middle of the gear ring 191, and the end of the intermediate rod 192 away from the gear ring 191 is fixedly connected to a circular ring 193, the internal rotation of the frame 11 is connected to a round rod 194, the round rod 194 passes through the frame 11 and extends to the inside of the frame 11, the outer side of the round rod 194 is fixedly connected to the circular ring 193, the circular ring 193 is sleeved on the outer side of the round rod 194, and the outer top of the round rod 194 is fixedly connected to a protrusion 195, the protrusion 195 is located on the side of the circular ring 193 away from the frame 11, the end of the round rod 194 is fixedly connected to a fixing plate 196, and the side of the fixing plate 196 close to the round rod 194 is fixed to the frame 11 is in contact with the outer side of the gear ring 191, and a cylinder 197 is fixedly connected to the side of the gear ring 191 close to the arc plate 13. The motor 16 is connected to an external power supply to work. The motor 16 drives the rotating shaft 17 to rotate, so that the rotating shaft 17 drives the outer gear 18 to rotate, and the gear 18 drives the gear ring 191 to rotate. At the same time, the cylinder 197 on the gear ring 191 slides inside the ring groove 15. At this time, the ring groove 15 provides a rigid guide for the swing of the gear ring 191 and the fermenter 3 to ensure that the fermenter 3 remains stable during the movement to avoid shaking or offset, and the cooperation between the cylinder 197 and the ring groove 15 can disperse mechanical stress, reduce the wear rate of moving parts, and extend the service life of the equipment. There are two cylinders 197, and the two cylinders 197 are symmetrically arranged at both ends of the gear ring 191. The cylinder 197 is located inside the ring groove 15.

[0032] The second embodiment, based on the first embodiment, see Figures 4 and 5As shown, the connector 2 includes a fixing ring 21, which is sleeved on the outside of the fermentation tank 3. The fixing ring 21 is fixedly connected to the fermentation tank 3. A fixing block 22 is fixedly connected to the middle of the outside of the fixing ring 21. There are two fixing blocks 22, which are symmetrically arranged with the fixing ring 21 as the center. The fixing block 22 is located on the outside of the fixing ring 21 close to the round rod 194. A square groove 23 is provided at one end of the fixing block 22 away from the fixing ring 21. The square groove 23 passes through the fixing block 22 and extends vertically downward. A contact plate 29 is slidably connected to the inside of the square groove 23. When the fermentation tank 3 is installed, the round rod 194 moves along the opening of the square groove 23 toward the inside of the square groove 23, so that the round rod 194 contacts and generates a contact between the inclined plate 28. The round rod 194 is squeezed and the inclined plate 28 is compressed, so that the round rod 194 passes smoothly through the inclined plates 28 symmetrically arranged on both sides of the square groove 23. Then, the end of the round rod 194 away from the fixed plate 196 is located inside the square groove 23 on the fixed block 22, and the round rods 194 on both sides are respectively located inside the square grooves 23 on both sides of the fixing ring 21. The elastic performance of the spring plate 210 is used to make the contact plate 29 and the round rod 194 in close contact. Due to long-term use, a wear gap will be generated between the round rod 194 and the contact plate 29, which can be adaptively compensated by the elastic deformation of the spring plate to prevent the fermenter 3 from loosening due to the expansion of the gap. At this time, the fermenter 3 and the connector 2 are clamped on the bracket 1 through the round rod 194 and the square groove 23. The interior of the square groove 23 is provided with an elastic The plate 210 and the two ends of the spring plate 210 are fixedly connected to the contact plate 29 and the inner wall of the square groove 23 respectively. A groove 27 is opened in the middle of the inner wall of the square groove 23, and the protrusion 195 is located inside the groove 27. The fermentation tank 3 and the connecting piece 2 are clamped on the bracket 1. The motor 16 is connected to the external power supply to work. The motor 16 drives the rotating shaft 17 to rotate, so that the rotating shaft 17 drives the outer gear 18 to rotate, and the gear 18 drives the gear ring 191 to rotate. The gear ring 191 drives the round rod 194 to rotate through the intermediate rod 192 and the ring 193, so that the round rod 194 drives the fixing ring 21 and the fixing block 22 to rotate through the protrusion 195, thereby driving the fermentation tank 3 to swing back and forth, thereby causing the ball 26 located on the outside of the tank body 31 to move along with the tank body 3. 1 also swings accordingly, causing a slight collision between the tank body 31 and the ball 26. The vibration generated by the collision on the outside of the tank body 31 will be transmitted to the tank wall, causing the sticky material attached to the inner wall of the tank body 31 to fall off, reducing the frequency of cleaning. The inner wall of the square groove 23 is fixedly connected with an inclined plate 28, which is elastic. There are two inclined plates 28. The inclined plates 28 are located at one end of the square groove 23 away from the groove 27, and the inclined plates 28 are symmetrically arranged inside the square groove 23. The outer side of the fixing ring 21 is fixedly connected with an extension block 24. There are two extension blocks 24, and the extension blocks 24 are alternately arranged with the fixed blocks 22. The bottom of the extension block 24 is fixedly connected with a spring 25, and the end of the spring 25 away from the extension block 24 is fixedly connected with the ball 26.

[0033] The third embodiment, based on the first and second embodiments, see Figures 6 to 8 As shown, the fermentation tank 3 comprises a tank body 31 fixedly connected with the fixed ring 21, a feed inlet 32 is arranged at the top of the tank body 31, an exhaust port 33 is fixedly connected with the top of the side of the tank body 31 away from the feed inlet 32, an electric motor 34 is fixedly connected with the middle of the top of the tank body 31, the output end of the electric motor 34 penetrates through the tank body 31 and extends to the inside of the tank body 31, a stirring piece 36 is arranged in the inside of the tank body 31, a discharge port 37 is arranged at the outside bottom of the tank body 31, the stirring piece 36 is fixedly connected with the electric motor 34, and a ring block 35 is fixedly connected with the inner wall of the middle of the tank body 31.

[0034] The stirring member 36 includes a rotating shaft 361, which is fixedly connected to the output end of the motor 34. An intermediate block 362 is fixedly connected to the middle of the outer side of the rotating shaft 361. A fixed rod 363 is fixedly connected to the outer side of the intermediate block 362. A slide groove 364 is provided on the end face of the fixed rod 363 away from the intermediate block 362. The ring block 35 is located inside the slide groove 364. There are three fixed rods 363, which are evenly distributed on the outer side of the intermediate block 362. The culture medium and cells are added to the interior of the tank body 31 through the feed port 32. The motor 34 is connected to an external power supply to work. The motor 34 drives the rotating shaft 361 to rotate. The rotating shaft 361 drives the intermediate block 362 in the middle and the sliders 365 at both ends to rotate, so that the intermediate block 362 The slider 365 drives the fixed rod 363 and the extension rod 366 to rotate respectively, stirring the culture medium inside the tank body 31 in the upper, middle and lower directions, and the extension rod 366 and the fixed rod 363 drive the spiral plate 367 and the curved plate 368 to rotate, so that the spiral plate 367 pushes the material along the axial direction of the tank body 31 to form an axial laminar flow, thereby pushing the bottom material to circulate to the top. At this time, the curved plate 368 generates a radial thrust opposite to the main spiral, forcing the material to diffuse in the direction away from the flow, forming radial turbulence, ensuring that the cells in each area can fully contact the nutrients, reducing cell death caused by nutrient deficiency or accumulation of metabolic waste, and at the same time, the interaction and offset of the two opposite spiral flows can reduce the shear force generated by stirring. Under the force environment, it can effectively improve the cell survival rate, maintain the normal morphology and function of cells, and reduce cell apoptosis or metabolic abnormalities caused by mechanical damage. The outer side of the rotating shaft 361 is rotatably connected to a slider 365. There are two sliders 365. The two sliders 365 are symmetrically arranged with the middle block 362 as the center. The outer side of the slider 365 is fixedly connected to an extension rod 366. There are multiple extension rods 366. The multiple extension rods 366 are evenly distributed on the outer side of the slider 365. The outer side of the rotating shaft 361 is provided with a spiral plate 367. There are multiple spiral plates 367. The multiple spiral plates 367 are divided into two groups. The two groups of spiral plates 367 are symmetrically arranged with the middle block 362 as the center. The two ends of the spiral plate 367 of one group are respectively connected to the fixed The top of the fixed rod 363 is fixedly connected to the extension rod 366 located above the middle block 362, and the two ends of the other group of spiral plates 367 are respectively fixedly connected to the bottom of the fixed rod 363 and the extension rod 366 located below the middle block 362. The outer side of the spiral plate 367 is fixedly connected to an arc plate 368, which is spirally arranged. The spiral direction of the arc plate 368 is opposite to that of the spiral plate 367. The bottom of the extension rod 366 is fixedly connected to a defoaming needle 369. The motor 34 is connected to an external power supply and drives the rotating shaft 361 to rotate. At this time, the rotating shaft 361 drives the slider 365 to rotate through the fixed column 3611 located inside the card slot 3612, so that the slider 365 drives the extension rod 366 and the defoaming needle 369 to rotate.The centrifugal force and tangential speed generated by the rotation of the defoaming needle 369 can exert shear force on the foam, causing it to burst. The gas generated during the fermentation process is discharged from the exhaust port 33 at the top of the tank body 31. The defoaming needle 369 is located on the extension rod 366 above the middle block 362. The outer end of the rotating shaft 361 is fixedly connected to a rotating plate 3610. There are multiple rotating plates 3610, which are symmetrically arranged at both ends of the rotating shaft 361. The interior of the slider 365 is fixedly connected to a fixing column 3611. The outer side of the rotating shaft 361 is provided with a slot 3612, and the fixing column 3611 is located inside the slot 3612.

[0035] During use, when the fermentation tank 3 is installed, the round rod 194 moves along the opening of the square groove 23 toward the inside of the square groove 23, so that the round rod 194 contacts and squeezes the inclined plate 28, and the inclined plate 28 is compressed, so that the round rod 194 passes smoothly through the inclined plates 28 symmetrically arranged on both sides of the square groove 23. Then, the end of the round rod 194 away from the fixed plate 196 is located inside the square groove 23 on the fixed block 22, and the round rods 194 on both sides are respectively located inside the square grooves 23 on both sides of the fixing ring 21. At this time, the fermentation tank 3 and the connecting piece 2 are clamped on the bracket 1 through the round rod 194 and the square groove 23.

[0036] The culture medium and cells are added to the interior of the tank body 31 through the feed port 32, and the motor 34 is connected to an external power supply to operate. The motor 34 drives the rotating shaft 361 to rotate, and the rotating shaft 361 drives the middle block 362 in the middle and the sliders 365 at both ends to rotate, so that the middle block 362 and the sliders 365 respectively drive the fixed rod 363 and the extension rod 366 to rotate, thereby stirring the culture medium inside the tank body 31 in three directions: upper, middle and lower.

[0037] At the same time, the motor 16 is connected to an external power supply and drives the rotating shaft 17 to rotate, so that the rotating shaft 17 drives the gears 18 at both ends to rotate. The rotation of the gear 18 drives the gear ring 191 meshing with the gear 18 to rotate, and then the gear ring 191 drives the intermediate rod 192 and the ring 193 to rotate, so that the ring 193 drives the round rod 194 to rotate. At this time, the round rod 194 drives the fermentation tank 3 to rotate through the connecting piece 2. As the motor 16 rotates forward and backward, the fermentation tank 3 swings back and forth. The periodic swing can avoid local accumulation of nutrients or metabolites in the culture medium inside the fermentation tank 3.

[0038] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or device comprising a series of elements includes not only those elements, but also other elements not explicitly listed, or elements inherent to such process, method, article, or device. In the absence of further limitations, an element defined by the phrase "comprising a..." does not exclude the presence of additional identical elements in the process, method, article, or device comprising the element.

[0039] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A fermentation device for cell culture that is easy to tilt, characterized in that: include: A fermentation tank (3), and a connecting piece (2) fixedly mounted on the outside; A bracket (1), wherein the bracket (1) is fixedly connected to the connecting member (2); The bracket (1) comprises a frame (11), the frame (11) is designed as a gantry frame, the outer side of the frame (11) is fixedly connected to a motor (16), the output end of the motor (16) passes through the frame (11), the inner wall of the frame (11) is rotatably connected to a rotating shaft (17), the two ends of the rotating shaft (17) are respectively rotatably connected to the two side edges of the frame (11), the rotating shaft (17) is fixedly connected to a gear (18) near the outer side of the frame (11), the inner wall of the frame (11) is rotatably connected to a rotating member (19), the rotating member (19) is meshed with the outer gear of the gear (18), and the rotating member (19) is clamped with the connecting member (2).

2. The cell culture fermentation equipment according to claim 1, wherein: The bottom of the frame (11) is fixedly connected with a fixing seat (14), the number of the fixing seats (14) is two, and the two fixing seats (14) are symmetrically arranged with the rotating shaft (17) as the center, the number of the gears (18) is two, and the two gears (18) are symmetrically arranged with the rotating shaft (17) as the center, the middle of the outer side of the frame (11) is fixedly connected with a connecting plate (12), the end of the connecting plate (12) away from the frame (11) is fixedly connected with an arc plate (13), and the number of the connecting plates (12) is Four, the four connecting plates (12) are divided into two groups, the connecting plates (12) in the two groups are symmetrically arranged on the two sides of the frame (11) with the rotating shaft (17) as the center, and the two connecting plates (12) in one group are symmetrically arranged with the motor (16) as the center, one end of the arc plate (13) is fixedly connected to one end of the connecting plate (12), the end of the arc plate (13) away from the connecting plate (12) is fixedly connected to the side of the frame (11), and a ring groove (15) is provided on the side of the arc plate (13) close to the rotating shaft (17).

3. The cell culture fermentation equipment according to claim 2, wherein: The rotating member (19) comprises a gear ring (191), the outer side of the gear ring (191) is meshed with the outer side of the gear (18), the side of the gear ring (191) away from the gear (18) is fixedly connected to an intermediate rod (192), the intermediate rod (192) is located in the middle of the gear ring (191), the end of the intermediate rod (192) away from the gear ring (191) is fixedly connected to a circular ring (193), the interior of the frame (11) is rotatably connected to a round rod (194), the round rod (194) passes through the frame (11) and extends to the interior of the frame (11), and the outer side of the round rod (194) is fixedly connected to the circular ring (193).

4. The cell culture fermentation equipment according to claim 3, wherein: The circular ring (193) is sleeved on the outside of the circular rod (194); a protrusion (195) is fixedly connected to the top of the outside of the circular rod (194); the protrusion (195) is located on the side of the circular ring (193) away from the frame (11); the end of the circular rod (194) is fixedly connected to a fixing plate (196); the side of the fixing plate (196) close to the circular rod (194) contacts the outside of the frame (11); the side of the gear ring (191) close to the arc plate (13) is fixedly connected to a cylinder (197); there are two cylinders (197); the two cylinders (197) are symmetrically arranged at both ends of the gear ring (191); the cylinders (197) are located inside the annular groove (15).

5. The cell culture fermentation equipment according to claim 4, wherein: The connecting member (2) includes a fixing ring (21), which is sleeved on the outer side of the fermentation tank (3). The fixing ring (21) is fixedly connected to the fermentation tank (3). A fixing block (22) is fixedly connected to the middle of the outer side of the fixing ring (21). There are two fixing blocks (22). The two fixing blocks (22) are symmetrically arranged with the fixing ring (21) as the center. The fixing blocks (22) are located on the outer side of the fixing ring (21) close to the round rod (194). A square groove (23) is provided at one end of the fixing block (22) away from the fixing ring (21). The square groove (23) passes through the fixing block (22) and extends vertically downward. A contact plate (29) is slidably connected to the interior of the square groove (23). A spring plate (210) is provided inside the square groove (23). The two ends of the spring plate (210) are fixedly connected to the contact plate (29) and the inner wall of the square groove (23) respectively.

6. The cell culture fermentation equipment according to claim 5, characterized in that: A groove (27) is provided in the middle of the inner wall of the square groove (23), and the protrusion (195) is located inside the groove (27). The inner wall of the square groove (23) is fixedly connected with an inclined plate (28), and there are two inclined plates (28). The inclined plates (28) are located at one end of the square groove (23) away from the groove (27), and the inclined plates (28) are symmetrically arranged inside the square groove (23). An extension block (24) is fixedly connected to the outer side of the fixing ring (21), and there are two extension blocks (24). The extension blocks (24) and the fixing blocks (22) are alternately arranged. The bottom of the extension block (24) is fixedly connected with a spring (25), and the end of the spring (25) away from the extension block (24) is fixedly connected with a ball (26).

7. The cell culture fermentation equipment according to claim 6, wherein: The fermentation tank (3) comprises a tank body (31), the tank body (31) is fixedly connected to a fixing ring (21), a feed port (32) is provided at the top of the tank body (31), an exhaust port (33) is fixedly connected to the top of the tank body (31) on a side away from the feed port (32), a motor (34) is fixedly connected to the middle of the top of the tank body (31), an output end of the motor (34) passes through the tank body (31) and extends to the inside of the tank body (31), a stirring member (36) is provided inside the tank body (31), a discharge port (37) is provided at the outer bottom of the tank body (31), the stirring member (36) is fixedly connected to the motor (34), and a ring block (35) is fixedly connected to the middle of the inner wall of the tank body (31).

8. The cell culture fermentation equipment according to claim 7, wherein: The stirring member (36) includes a rotating shaft (361), the rotating shaft (361) is fixedly connected to the output end of the motor (34), an intermediate block (362) is fixedly connected to the middle of the outer side of the rotating shaft (361), a fixing rod (363) is fixedly connected to the outer side of the intermediate block (362), and a sliding groove (364) is provided on the end surface of the fixing rod (363) away from the intermediate block (362), the ring block (35) is located inside the sliding groove (364), and there are three fixing rods (363), which are evenly distributed on the outer side of the intermediate block (362).

9. The cell culture fermentation equipment according to claim 8, characterized in that: The outer side of the rotating shaft (361) is rotatably connected to a slider (365), and there are two sliders (365). The two sliders (365) are symmetrically arranged with the middle block (362) as the center. The outer side of the slider (365) is fixedly connected to an extension rod (366), and there are multiple extension rods (366). The multiple extension rods (366) are evenly distributed on the outer side of the slider (365). The outer side of the rotating shaft (361) is provided with a spiral plate (367). There are multiple plates (367), and the multiple spiral plates (367) are divided into two groups. The two groups of spiral plates (367) are symmetrically arranged with the middle block (362) as the center. The two ends of the spiral plates (367) in one group are fixedly connected to the fixed rod (363) and the extension rod (366), respectively. The outer side of the spiral plate (367) is fixedly connected with an arc plate (368), and the arc plate (368) is spirally arranged. The spiral direction of the arc plate (368) is opposite to that of the spiral plate (367).

10. The cell culture fermentation equipment according to claim 9, characterized in that: The bottom of the extension rod (366) is fixedly connected to a defoaming needle (369), and the defoaming needle (369) is located on the extension rod (366) above the middle block (362). The outer side of the end of the rotating shaft (361) is fixedly connected to a rotating plate (3610), and there are multiple rotating plates (3610), and the multiple rotating plates (3610) are symmetrically arranged at both ends of the rotating shaft (361). The inside of the slider (365) is fixedly connected to a fixed column (3611), and a slot (3612) is provided on the outer side of the rotating shaft (361), and the fixed column (3611) is located inside the slot (3612).

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