Tablet making and dyeing integrated machine

Abstract

The application discloses a dyeing and slice-making integrated machine, and relates to the technical field of slice-making and dyeing. The application comprises an integrated box for preparing a dyed slide, and further comprises a transverse moving assembly, a feeding unit, a preparation platform and a preparation unit. The transverse moving assembly is internally provided with a longitudinal sliding plate. The feeding unit is internally provided with a dyeing box and a sample adding box. The dyeing box is internally provided with a dyeing needle group for adding dyeing liquid through a dyeing assembly. The preparation platform is provided with the preparation unit. The preparation unit comprises a centrifugal assembly, a bearing assembly and a backwashing assembly. The application can realize the automatic slice-making process of the storage and loading of cell samples, centrifugal concentration and dyeing, the overall processing time is shorter, the sample adding accuracy and quality stability are significantly improved, the dyeing liquid input can be accurately controlled, the dyeing liquid mixing can be avoided, the multi-dyeing accuracy is higher, the internal and external cleaning of the dyeing needle group and the sample adding needle group can be realized, the cross contamination is not easy to occur, and the accuracy of analysis results is effectively improved.

Description

Technical Field

[0001] This invention relates to the technical field of slide preparation and dyeing, and more particularly to an integrated slide preparation and dyeing machine. Background Technology

[0002] In current clinical pathology diagnosis, cell biology and other fields, when conducting pathological studies of cells, slide preparation and staining machines are usually used to prepare and stain cell slides in order to observe whether there is infection or tissue lesions in the cell samples.

[0003] Existing slide staining machines employ various methods to prepare and stain cell samples. For example, a slide staining integrated machine with publication number CN109297792A includes a main frame, a robotic arm device, and a staining sliding mechanism. The robotic arm device includes a first vertical sliding mechanism and a first robotic arm mechanism. The two ends of the first vertical sliding mechanism are respectively connected to the upper end of the main frame, and the first robotic arm mechanism is slidably connected to the first vertical sliding mechanism. The staining sliding mechanism includes a staining sliding support, a longitudinal sliding plate, and a staining plate. The two ends of the staining sliding support are respectively connected to the lower end of the main frame, and the longitudinal sliding plate is horizontally and longitudinally slidably connected to the staining sliding support. The staining plate is installed on the top surface of the longitudinal sliding plate.

[0004] Traditional cell smearing, fixation, and staining processes require manual completion of each step sequentially, which is cumbersome and time-consuming. Manual operation is easily affected by the operator's skill level, experience, and subjective factors, resulting in inconsistent sample processing quality. For example, the existing technology mentioned above cannot quickly stain and add multiple samples. In addition, the device has a single function, only performing Papanicolaou staining, and cannot achieve multiple staining and avoid the problem of mixing different staining solutions. Furthermore, the device cannot achieve real-time cleaning of the staining needles, which can easily lead to cross-contamination during slide preparation, thus having certain limitations.

[0005] Therefore, there is an urgent need to design an integrated film preparation and dyeing machine to solve the above problems. Summary of the Invention

[0006] To address the shortcomings of existing technologies, this invention provides an integrated slide preparation and dyeing machine, which solves the problems mentioned in the background section.

[0007] To achieve the above objectives, the present invention provides the following technical solution: an integrated slide preparation and staining machine, comprising an integrated box for preparing stained glass slides, and further comprising:

[0008] The transverse component is housed within an integrated box, which contains a longitudinal sliding plate. The transverse component is used to enable the transverse and longitudinal movement of the longitudinal sliding plate.

[0009] The feeding unit is located on a longitudinal sliding plate and contains a staining box and a sample loading box. The staining box contains a set of staining needles for adding dye solution through a staining component, and the sample loading box contains a set of sample loading needles for sample aspiration and loading through a sample loading component. A cleaning component is provided between the staining box and the sample loading box.

[0010] The preparation platform is housed within an integrated box, and preparation units are installed on the preparation platform;

[0011] The preparation unit includes a centrifugation assembly, a carrier assembly, and a backwashing assembly. The centrifugation assembly contains multiple centrifuge boxes for centrifuging and concentrating samples. The backwashing assembly is driven by the centrifugation assembly to backwash and clean the staining needle assembly and the sample dispensing needle assembly.

[0012] Preferably, the integrated box is equipped with a control display screen, which is used to control the opening and closing and operating status of the transverse component, the feeding unit and the preparation unit, so as to realize the automated preparation of stained glass slides.

[0013] Preferably, the support assembly includes a slide preparation plate, a pipette holder, and a sample tray disposed on the preparation platform, wherein the slide preparation plate is used to place multiple stained slides to be prepared, the pipette holder is used to place clean pipette tips to be used, and the sample tray is used to place multiple sample tubes to be sampled.

[0014] Preferably, the lateral movement component includes a lateral moving platform disposed within an integrated housing, a longitudinal moving platform disposed on the lateral moving platform for realizing the lateral movement of the longitudinal moving platform, and a longitudinal sliding plate disposed on the longitudinal moving platform for realizing the longitudinal movement of the longitudinal sliding plate.

[0015] Preferably, the dyeing assembly includes two electrically operated telescopic rods fixedly installed inside the dyeing box. Each of the two electrically operated telescopic rods is fixedly equipped with a lifting frame, and both lifting frames are slidably connected to the dyeing box. Each of the two lifting frames is fixedly equipped with a lifting rod, and each of the two lifting rods is provided with two dyeing needle groups for adding different dye solutions.

[0016] The integrated box is equipped with a dyeing telescopic tube, and two dyeing injection tubes are installed inside the dyeing telescopic tube. The dyeing box is equipped with a connecting pipe that is connected to the two dyeing injection tubes. Two dispensing tubes are fixedly connected to the connecting pipe, and each dispensing tube is connected to two corresponding dyeing needle groups.

[0017] Preferably, the sample loading assembly includes two electrically operated telescopic rods 2 fixedly installed inside the sample loading box. Each of the two electrically operated telescopic rods 2 has a lifting frame 2 fixedly installed at its lower end. Each of the two lifting frames 2 has a sample loading needle assembly fixedly installed on it. The sample loading box contains a sample loading cylinder. The sample loading cylinder has two telescopic conduits fixedly connected to it, and the two telescopic conduits are respectively connected to two sample loading needle assemblies. The sample loading cylinder and the integrated box are fixedly connected by a sample loading telescopic tube.

[0018] Preferably, the cleaning assembly includes a rinsing cylinder fixedly installed on a longitudinal sliding plate, and a second water guide pipe is fixedly connected between the rinsing cylinder and the connecting pipe. A first water guide pipe is fixedly connected between the rinsing cylinder and the sample loading cylinder, and both the first and second water guide pipes are equipped with control valves. A connecting water supply pipe for water supply is fixedly connected to the lower part of the rinsing cylinder.

[0019] Preferably, the centrifugation assembly includes a centrifuge box fixedly installed on the preparation platform, a servo motor fixedly installed inside the centrifuge box, a drive roller fixedly installed on the drive end of the servo motor, a centrifuge rack fixedly installed on the drive roller, and multiple centrifuge boxes provided on the centrifuge rack, with multiple centrifuge tubes for placing samples provided on the centrifuge boxes.

[0020] Preferably, the backwashing assembly includes a waste liquid tank for collecting waste liquid, which is set on the preparation platform. A sewage support plate is provided on the waste liquid tank. Two water injection pipes for water supply are fixedly connected to the waste liquid tank, and both water injection pipes are connected to a water supply pipe. Two waste liquid discharge pipes are fixedly connected to the waste liquid tank, and the inlet ends of the two waste liquid discharge pipes are located on the upper part of the sewage support plate. A water supply mechanism is provided inside the waste liquid tank.

[0021] Preferably, the water supply mechanism includes a linkage roller rotatably installed between the sewage support plate and the waste liquid tank, and a transmission belt is sleeved between the linkage roller and the drive roller. A water squeezing plate is slidably installed inside the sewage support plate, and cleaning water is filled between the water squeezing plate and the sewage support plate. A water replenishment pipe for replenishing cleaning water is fixedly connected to the waste liquid tank.

[0022] Multiple rinsing pipes for rinsing the staining needle assembly and the sample application needle assembly are fixedly connected on the wastewater support plate, and the inlet ends of the multiple rinsing pipes and the two water injection pipes are all located between the squeezing plate and the wastewater support plate. A reciprocating screw is fixedly installed on the linkage roller, and a ball bearing nut plate is fixedly installed on the squeezing plate, and the ball bearing nut plate cooperates with the reciprocating screw.

[0023] This invention provides an integrated slide preparation and dyeing machine. It has the following beneficial effects:

[0024] 1. When preparing stained slides, this slide preparation and staining machine can flexibly adjust the lateral and longitudinal positions of the sampling needle through the cooperation of the transverse component and the sample loading component, so as to quickly realize the extraction, transfer and loading of different cell samples, with high loading accuracy and higher sample quality stability.

[0025] 2. When preparing stained slides, this slide preparation and staining machine can simultaneously perform Papanicolaou staining and FSC staining (FSC staining is also known as FSC-811 staining, where FSC stands for Forward Scatter, a cell staining technique used for cervical lesion screening) by using different staining needle sets. It offers a wider range of staining options, and the independent staining with different staining needle sets effectively avoids the problem of mixed contamination of different staining solutions, resulting in better staining effects.

[0026] 3. When preparing stained slides, this slide preparation and staining machine can quickly transfer cell samples into centrifuge tubes through the combination of the sample dispensing needle assembly and the centrifugation component. The high-speed rotation can quickly achieve simultaneous centrifugation of multiple cell samples, resulting in high concentration of cell samples and separation of impurities, making the test results more accurate.

[0027] 4. When preparing stained slides, this slide preparation and staining machine can automatically rinse the external parts of the staining needle group and the sample dispensing needle group during centrifugation. At the same time, it can backwash the internal conveying pipes of the staining needle group and the sample dispensing needle group, thus achieving internal and external cleaning of the staining needle group and the sample dispensing needle group at the same time. The cleaning effect is better and cross-contamination is less likely to occur.

[0028] In summary, this invention enables automated slide preparation processes for cell sample storage, loading, centrifugation, concentration, and staining, resulting in shorter overall processing time, significantly improved sample loading accuracy and quality stability, precise control of staining solution input, prevention of staining solution mixing, higher accuracy in multiple staining, and internal and external cleanliness of the staining needle assembly and sample loading needle assembly, reducing the risk of cross-contamination and effectively improving the accuracy of analytical results.

[0029] Other features and advantages of the invention will be set forth in the description which follows, and will be apparent in part from the description, or may be learned by practicing the invention. The objects and other advantages of the invention may be realized and obtained by means of the structures particularly pointed out in the written description and the accompanying drawings. Attached Figure Description

[0030] The specific embodiments of the present invention will be further described in detail below with reference to the accompanying drawings, wherein:

[0031] Figure 1 This is a schematic diagram of the structure of an integrated slide preparation and dyeing machine proposed in this invention;

[0032] Figure 2 for Figure 1 A schematic diagram of the structure after rotation at a certain angle;

[0033] Figure 3 for Figure 2 A schematic diagram of the internal structure of the integrated box after part of it has been removed.

[0034] Figure 4 for Figure 3 A schematic diagram of the structure after removing the integrated box;

[0035] Figure 5 for Figure 4 Schematic diagram of the transverse and longitudinal moving stages;

[0036] Figure 6 for Figure 5 Schematic diagram of the staining chamber and sample loading chamber;

[0037] Figure 7 for Figure 6 A schematic diagram of the structure after rotation at a certain angle;

[0038] Figure 8 for Figure 6 Schematic diagram of the internal structure of the dyeing chamber;

[0039] Figure 9 for Figure 6 Internal structure diagram of the Sino-Canadian sample box;

[0040] Figure 10 for Figure 7 A schematic diagram of the structure of the cleaning component;

[0041] Figure 11 for Figure 4 A schematic diagram of the preparation platform in the middle;

[0042] Figure 12 for Figure 11 Schematic diagram of the structure of the centrifuge box and waste liquid tank;

[0043] Figure 13 for Figure 12 Schematic diagram of the internal structure of the centrifuge chamber and waste liquid tank;

[0044] Figure 14 for Figure 13 Schematic diagram of the internal structure of the waste liquid tank;

[0045] Figure 15 for Figure 14 Front view of the waste liquid tank.

[0046] In the diagram: 1. Integrated box, 2. Control display screen, 3. Preparation platform, 4. Sample dispensing telescopic tube, 5. Staining telescopic tube, 6. Centrifuge box, 7. Slide preparation plate, 8. Suction nozzle holder, 9. Horizontal moving stage, 10. Longitudinal moving stage, 11. Limiting slide, 12. Longitudinal sliding plate, 13. Staining box, 14. Sample dispensing box, 15. Lifting rod, 16. Staining needle assembly, 17. Sample dispensing needle assembly, 18. Rinsing cylinder, 19. Connecting water supply pipe, 20. Water guide pipe rack one, 21. Water guide pipe rack two, 22. Electric telescopic rod one, 23. Lifting frame one, 24. Sample dispensing cylinder, 25. Electric telescopic rod two, 26. Lifting frame two, 27. Sample tray, 28. Waste liquid tank, 29. Water injection conduit, 30. Centrifuge rack, 31. Centrifuge box, 32. Servo motor, 33. Transmission belt, 34. Squeezing plate, 35. Reciprocating screw, 36. Ball bearing nut plate, 37. Wastewater carrying plate, 38. Rinsing pipe, 39. Water replenishment pipe, 40. Linkage roller. Detailed Implementation

[0047] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0048] Example 1: Refer to Figures 1-5 as well as Figure 11 A slide preparation and staining integrated machine includes an integrated box 1 for preparing stained glass slides.

[0049] This integrated film preparation and dyeing machine also includes:

[0050] A transverse component is disposed inside an integrated box 1, which contains a longitudinal sliding plate 12. The transverse component is used to realize the transverse and longitudinal movement of the longitudinal sliding plate 12.

[0051] The transverse movement assembly includes a transverse moving stage 9 disposed within an integrated housing 1, a longitudinal moving stage 10 disposed on the transverse moving stage 9, the transverse moving stage 9 being used to realize the transverse movement of the longitudinal moving stage 10, and a longitudinal sliding plate 12 disposed on the longitudinal moving stage 10, the longitudinal moving stage 10 being used to realize the longitudinal movement of the longitudinal sliding plate 12.

[0052] Both the transverse moving stage 9 and the longitudinal moving stage 10 are commonly used devices for driving displacement in the prior art. They are both equipped with drive motors for driving. When the transverse moving stage 9 is started, it drives the longitudinal moving stage 10 to move horizontally on the transverse moving stage 9. When the longitudinal moving stage 10 is started, it drives the longitudinal sliding plate 12 to move horizontally on the longitudinal moving stage 10. The two work together to adjust the horizontal and vertical positions of the longitudinal sliding plate 12, so that cell samples at different positions in the integrated box 1 can be transferred or stained.

[0053] Both the horizontal moving stage 9 and the vertical moving stage 10 are existing technologies and will not be described in detail here.

[0054] The feeding unit is located on the longitudinal sliding plate 12. The feeding unit is used to extract and transfer multiple cell samples and inject different cell stains precisely, thereby improving the accuracy of the slide preparation and staining process and reducing the error in staining and slide preparation.

[0055] The preparation platform 3 is set inside the integrated box 1, and the preparation platform 3 is equipped with a preparation unit. The preparation unit includes a centrifugation component, a carrier component and a backwashing component. The preparation unit is used to carry cell samples, centrifuge and concentrate them and place them on glass slides, and cooperates with the feeding unit to realize the automatic addition of staining solution on the glass slides.

[0056] The supporting components include a slide preparation plate 7, a pipette holder 8, and a sample tray 27 set on the preparation platform 3. The slide preparation plate 7 is used to place multiple stained glass slides to be prepared, the pipette holder 8 is used to place clean pipette tips to be used, and the sample tray 27 is used to place multiple sample tubes to be sampled.

[0057] The slide preparation plate 7 is used to place multiple staining slides and to add cell samples and staining solution, thereby realizing the automatic preparation of cell staining slides.

[0058] The nozzle holder 8 is used to hold multiple clean nozzles, which can be replaced in time during slide preparation and staining to avoid cross-contamination. At the same time, the preparation platform 3 is equipped with a nozzle collection box to collect discarded nozzles.

[0059] Multiple different cell samples are placed on the sample tray 27, which facilitates the extraction by the sample loading unit to automatically prepare different stained slides.

[0060] The integrated box 1 is equipped with a control display screen 2, which is used to control the opening and closing and operation status of the transverse component, the feeding unit and the preparation unit, so as to realize the automated preparation of stained glass slides, improve the efficiency and accuracy of slide preparation and staining, and avoid errors.

[0061] Example 2: Refer to Figures 3-9 The technical solution that differs from that of Embodiment 1 is as follows: the feeding unit is provided with a dyeing box 13 and a sample feeding box 14. The dyeing box 13 is provided with a dyeing needle group 16 for adding dye solution through the dyeing component. The sample feeding box 14 is provided with a sample feeding needle group 17 for sample aspiration and feeding through the sample feeding component. A cleaning component is provided between the dyeing box 13 and the sample feeding box 14.

[0062] The dyeing assembly includes two electric telescopic rods 22 fixedly installed inside the dyeing box 13. Each of the two electric telescopic rods 22 is fixedly installed with a lifting frame 23, and both lifting frames 23 are slidably connected to the dyeing box 13. Each of the two lifting frames 23 is fixedly installed with a lifting rod 15, and each of the two lifting rods 15 is provided with two dyeing needle groups 16 for adding different dye solutions.

[0063] When the electric telescopic rod 22 is started, it will drive the lifting frame 23 to rise and fall. When the lifting frame 23 rises and falls, it will simultaneously drive the lifting rod 15 to rise and fall, which in turn drives the staining needle assembly 16 to rise and fall. This allows the height position of the staining needle assembly 16 to be adjusted so that it can be moved down into the interior of the cell sample for precise application of the staining solution without any displacement of the application position, resulting in more accurate application.

[0064] The integrated box 1 is equipped with a dyeing telescopic tube 5, and two dyeing injection tubes are installed inside the dyeing telescopic tube 5. The dyeing box 13 is equipped with a connecting tube that is connected to the two dyeing injection tubes. Two dispensing tubes are fixedly connected to the connecting tube, and each dispensing tube is connected to the corresponding two dyeing needle groups 16.

[0065] The two connecting tubes are used to add Papanicolaou stain and FSC stain respectively, so that Papanicolaou stain and FSC stain can be performed simultaneously. This allows for more types of staining and stronger functionality. The stain enters the separatory tube through the connecting tube and then enters different staining needle groups 16. This allows for independent injection of Papanicolaou stain and FSC stain without affecting each other and without causing stain mixing or contamination.

[0066] In a further embodiment, the sample loading assembly includes two electric telescopic rods 25 fixedly installed inside the sample loading box 14. The lower ends of the two electric telescopic rods 25 are each fixedly mounted with a lifting frame 26. Each of the two lifting frames 26 is fixedly mounted with a sample loading needle assembly 17. The sample loading box 14 is provided with a sample loading cylinder 24. Two telescopic conduits are fixedly connected to the sample loading cylinder 24, and the two telescopic conduits are respectively connected to the two sample loading needle assemblies 17. A sample loading telescopic tube 4 is fixedly connected between the sample loading cylinder 24 and the integrated box 1.

[0067] When the electric telescopic rod 25 is started, it will drive the lifting frame 26 to rise and fall. When the lifting frame 26 rises and falls, it will drive the sample application needle assembly 17 on it to rise and fall, which can in turn retract or stretch the telescopic catheter, so that the sample application needle assembly 17, the telescopic catheter and the sample application tube 24 are always connected. This allows cell samples to be extracted and transferred through the sample application telescopic tube 4, achieving precise sample application or retrieval.

[0068] Both the staining needle assembly 16 and the sample dispensing needle assembly 17 are equipped with control valves and are wirelessly controlled by the controller in the control display screen 2. This allows for precise control of the input of different staining solutions or cell samples. At the same time, both the staining needle assembly 16 and the sample dispensing needle assembly 17 are connected to a sealing ball by a spring, which can achieve the function of controlling the flow rate of staining solutions or samples and preventing backflow, thus realizing the precise input of staining solutions or samples.

[0069] Example 3: Refer to Figures 3-6 as well as Figures 10-15 The difference between this embodiment and embodiment two is that: the centrifugation assembly is provided with multiple centrifugation boxes 31 for sample centrifugation concentration, and the backwashing assembly is driven by the centrifugation assembly to achieve backwashing and cleaning of the staining needle group 16 and the sample loading needle group 17.

[0070] The centrifugation assembly includes a centrifuge box 6 fixedly installed on the preparation platform 3. A servo motor 32 is fixedly installed inside the centrifuge box 6, and a drive roller is fixedly installed on the drive end of the servo motor 32. A centrifuge rack 30 is fixedly installed on the drive roller, and multiple centrifuge boxes 31 are provided on the centrifuge rack 30. Multiple centrifuge tubes for placing samples are provided on the centrifuge boxes 31.

[0071] After the cell samples are placed in the sample tray 27, multiple cell samples can be sequentially extracted from the sample tray 27 using the sample dispensing needle assembly 17. The sample dispensing needle assembly 17 is then moved to the upper part of the centrifuge tube at the corresponding position on the centrifuge box 31 using the lateral movement component. The cell samples are then injected into the centrifuge tube using the sample dispensing needle assembly 17. By repeating the above steps, multiple cell samples can be sequentially placed into different centrifuge tubes. After closing the centrifuge tube, the centrifugation and concentration of the cell samples can begin.

[0072] During centrifugation, the servo motor 32 is activated to drive the drive roller on the drive end to rotate. When the drive roller rotates, it drives the centrifuge rack 30 to rotate. The rotation of the centrifuge rack 30 drives multiple centrifuge boxes 31 to rotate, which in turn drives multiple centrifuge tubes on them to rotate. During the rotation, the cell samples inside are centrifuged, causing the cell samples to separate into supernatant and cell clusters at the bottom. After the supernatant is removed, the cell clusters are extracted and injected onto the staining slide on the slide preparation plate 7, thus completing the cell sample injection.

[0073] After the cell sample is injected, Papanicolaou stain or FSC stain can be precisely injected through staining needle group 16 to complete the staining process of the slide.

[0074] In a further embodiment, the backwashing assembly includes a waste liquid tank 28 disposed on the preparation platform 3 for collecting waste liquid. A wastewater support plate 37 is disposed on the waste liquid tank 28. The upper space of the wastewater support plate 37 in the waste liquid tank 28 is used to store staining waste liquid or sample waste liquid to avoid cross-contamination caused by waste liquid dripping.

[0075] Two waste liquid drain pipes are fixedly connected to the waste liquid tank 28, and the inlet ends of the two waste liquid drain pipes are located on the upper part of the wastewater support plate 37. After the film is made, the waste liquid on the wastewater support plate 37 can be discharged and collected through the waste liquid drain pipes.

[0076] The waste liquid tank 28 is equipped with a water supply mechanism, which includes a linkage roller 40 rotatably installed between the sewage support plate 37 and the waste liquid tank 28, and a transmission belt 33 is sleeved between the linkage roller 40 and the drive roller. A squeezing plate 34 is slidably installed in the sewage support plate 37. A reciprocating screw 35 is fixedly installed on the linkage roller 40. A ball nut disc 36 is fixedly installed on the squeezing plate 34, and the ball nut disc 36 cooperates with the reciprocating screw 35.

[0077] The rotation of the drive roller will drive the linkage roller 40 to rotate under the action of the transmission belt 33. The rotation of the linkage roller 40 will drive the reciprocating screw 35 on it to rotate. When the reciprocating screw 35 rotates, it will drive the ball nut disc 36 on it to perform reciprocating lifting and lowering motion, which will in turn drive the dewatering plate 34 to reciprocate lifting and lowering within the waste liquid tank 28.

[0078] The cooperation between the reciprocating lead screw 35 and the ball nut disc 36 is existing technology. The specific principle is as follows: the reciprocating lead screw 35 is a form of three-dimensional cam pair. Its specific structure consists of two threaded grooves with the same pitch and opposite directions of rotation. The two ends of the lead screw are connected by a transition curve. By rotating the lead screw, the side of the helical groove pushes the balls located in the helical groove of the ball nut disc 36 to make axial reciprocating motion. The reciprocating motion of the balls will drive the ball nut disc 36 to reciprocate. That is, when the reciprocating lead screw 35 rotates in one direction, it can drive the ball nut disc 36 to make it reciprocate linearly on the reciprocating lead screw 35.

[0079] The space between the squeezing plate 34 and the sewage carrying plate 37 is filled with cleaning water. A water supply pipe 39 for replenishing cleaning water is fixedly connected to the waste liquid tank 28. When the squeezing plate 34 moves upward, it will squeeze the cleaning water on its upper part. When the squeezing plate 34 moves downward, it will stop squeezing the cleaning water. At the same time, the water supply pipe 39 will inject cleaning water for real-time replenishment.

[0080] In a further embodiment, a plurality of rinsing tubes 38 for rinsing the staining needle assembly 16 and the sample dispensing needle assembly 17 are fixedly connected on the wastewater support plate 37, and the inlet ends of the plurality of rinsing tubes 38 and the two water injection conduits 29 are all located between the squeezing plate 34 and the wastewater support plate 37.

[0081] When the squeezing plate 34 squeezes the cleaning water, it will squeeze the cleaning water through the lower end of the rinsing pipe 38, and then spray the cleaning water through the rinsing pipe 38 onto the upper part of the wastewater carrying plate 37. During the centrifugation process, the staining needle group 16 or the sample dispensing needle group 17 can be moved by the transverse moving component, so that the staining needle group 16 or the sample dispensing needle group 17 moves into the waste liquid tank 28. At this time, the cleaning water flushed out by multiple rinsing pipes 38 will rinse the outside of the staining needle group 16 or the sample dispensing needle group 17, thereby washing away the waste liquid attached to the outside of the staining needle group 16 or the sample dispensing needle group 17 and letting the waste liquid fall into the waste liquid tank 28 for collection. This completes the external cleaning of the staining needle group 16 or the sample dispensing needle group 17 and avoids cross-contamination.

[0082] The cleaning assembly includes a rinsing cylinder 18 fixedly installed on a longitudinal sliding plate 12, and a second water guide pipe bracket 21 fixedly connected between the rinsing cylinder 18 and the connecting pipe. A first water guide pipe bracket 20 is fixedly connected between the rinsing cylinder 18 and the sample filling cylinder 24. Both the first water guide pipe bracket 20 and the second water guide pipe bracket 21 are equipped with control valves. A connecting water supply pipe 19 for water supply is fixedly connected to the lower part of the rinsing cylinder 18. Two water injection pipes 29 for water supply are fixedly connected to the waste liquid tank 28, and both water injection pipes 29 are matched with the connecting water supply pipe 19.

[0083] During the external rinsing of the staining needle assembly 16, the downward movement of the staining chamber 13 will cause the connecting water pipe 19 to move downward as well, and cause the connecting water pipe 19 to engage with the water injection conduit 29 on the right side (as per the instruction manual). Figure 12 The water injection conduit 29 is located on the right side. When the squeezing plate 34 squeezes the cleaning water, the cleaning water will be injected into the water supply pipe 19 through the water injection conduit 29 on the right side, and enter the water guide frame 1 20 and water guide frame 21 through the rinsing cylinder 18. The cleaning water in the water guide frame 1 20 will be injected into the sample needle assembly 17 through the sample cylinder 24, and discharged into the waste liquid tank 28 through the sample needle assembly 17. In this way, the inside of the sample needle assembly 17 can be rinsed during the flow of cleaning water.

[0084] The cleaning water in the water guide tube 21 will be injected into the two staining needle groups 16 through the connecting tube, and discharged into the waste liquid tank 28 through the staining needle group 16. In the process of the cleaning water flow, the inside of the staining needle group 16 will be rinsed, thereby realizing the internal rinsing of the staining needle group 16 and the sample loading needle group 17, so that no staining liquid or sample remains inside, thus avoiding cross-contamination.

[0085] During the external rinsing of the sample injection needle assembly 17, the downward movement of the staining chamber 13 will cause the connecting water inlet pipe 19 to move downward as well, and cause the connecting water inlet pipe 19 to engage with the water injection conduit 29 on the left side (as per the instruction manual). Figure 12When the water injection conduit 29 is located on the left side, the water squeezing plate 34 squeezes the cleaning water, and the cleaning water will be injected into the water supply pipe 19 through the water injection conduit 29 on the left side. The above cleaning steps can be repeated to perform a second rinsing of the inside of the staining needle group 16 and the sample dispensing needle group 17, thereby improving the rinsing effect and improving the cleanliness of the inside of the staining needle group 16 and the sample dispensing needle group 17.

[0086] The specific working principle of this film preparation and dyeing integrated machine is as follows:

[0087] Multiple cell samples to be prepared are sequentially placed into the sample tray 27. After the cell samples are placed, the sample dispensing needle assembly 17 is used to extract the multiple cell samples from the sample tray 27 in sequence. The dispensing needle assembly 17 is then moved to the upper part of the centrifuge tube at the corresponding position on the centrifuge box 31 by the cooperation of the transverse component. The cell samples are then injected into the centrifuge tube by the dispensing needle assembly 17. The above steps are repeated to place multiple cell samples into different centrifuge tubes in sequence. The centrifuge tubes are then closed to begin the centrifugation and concentration of the cell samples.

[0088] The servo motor 32 is started to drive the drive roller on the drive end to rotate. When the drive roller rotates, it will drive the centrifuge rack 30 to rotate. The rotation of the centrifuge rack 30 will drive multiple centrifuge boxes 31 to rotate, which in turn will drive multiple centrifuge tubes on them to rotate. During the rotation, the cell samples inside are centrifuged, and the cell samples are separated into supernatant and cell clusters at the bottom. After the supernatant is removed, the cell clusters are extracted and injected onto the staining slide on the slide preparation plate 7, thus completing the cell sample injection.

[0089] After the cell sample is injected, Papanicolaou stain or FSC stain can be precisely injected through staining needle group 16 to complete the staining process of the slide.

[0090] During cell centrifugation, the water supply mechanism drives the squeezing plate 34 to move back and forth, while the transverse component controls the movement of the staining needle assembly 16 or the sample dispensing needle assembly 17, moving them into the waste liquid tank 28. The reciprocating movement of the squeezing plate 34 forces the cleaning water out through multiple rinsing tubes 38 and the water injection conduit 29. The cleaning water flushed out by the multiple rinsing tubes 38 rinses the exterior of the staining needle assembly 16 or the sample dispensing needle assembly 17, removing the waste liquid adhering to the exterior of the staining needle assembly 16 or the sample dispensing needle assembly 17 and allowing the waste liquid to fall into the waste liquid tank 28 for collection. This completes the external cleaning of the staining needle assembly 16 or the sample dispensing needle assembly 17, preventing cross-contamination.

[0091] The cleaning water pressurized from the water injection conduit 29 is injected into the water supply pipe 19 through the water injection conduit 29, and then enters the water guide frame 20 and the water guide frame 21 through the rinsing tube 18. This allows the inside of the staining needle assembly 16 and the sample application needle assembly 17 to be rinsed, so that no staining solution or sample remains inside, thus avoiding cross-contamination.

[0092] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A slide preparation and staining integrated machine, comprising an integrated box (1) for preparing stained glass slides, characterized in that, Also includes: A transverse component is set inside an integrated box (1), which contains a longitudinal sliding plate (12). The transverse component is used to realize the transverse and longitudinal movement of the longitudinal sliding plate (12). The feeding unit is set on the longitudinal sliding plate (12), and a staining box (13) and a sample loading box (14) are set inside it. The staining box (13) is equipped with a staining needle group (16) for adding dye solution through the staining component. The sample loading box (14) is equipped with a sample loading needle group (17) for sample aspiration and loading through the sample loading component. A cleaning component is set between the staining box (13) and the sample loading box (14). The preparation platform (3) is set inside the integrated box (1), and the preparation platform (3) is provided with preparation units; The preparation unit includes a centrifugation assembly, a carrier assembly and a backwashing assembly. The centrifugation assembly is equipped with multiple centrifugation boxes (31) for centrifugation concentration of samples. The backwashing assembly is driven by the centrifugation assembly to perform backwashing and cleaning of the staining needle group (16) and the sample dispensing needle group (17). The backwashing assembly includes a waste liquid tank (28) for collecting waste liquid, which is set on the preparation platform (3). A sewage support plate (37) is set on the waste liquid tank (28). Two water injection pipes (29) for water supply are fixedly connected on the waste liquid tank (28). A water supply mechanism is set inside the waste liquid tank (28). The water supply mechanism includes a linkage roller (40) rotatably installed between the sewage support plate (37) and the waste liquid tank (28), and a transmission belt (33) is sleeved between the linkage roller (40) and the drive roller. A water squeezing plate (34) is slidably installed inside the sewage support plate (37), and cleaning water is filled between the water squeezing plate (34) and the sewage support plate (37). A water replenishment pipe (39) for replenishing cleaning water is fixedly connected to the waste liquid tank (28). The wastewater support plate (37) is fixedly connected to multiple rinsing pipes (38) for rinsing the dyeing needle group (16) and the sample injection needle group (17), and the inlet ends of the multiple rinsing pipes (38) and the two water injection pipes (29) are located between the squeezing plate (34) and the wastewater support plate (37). The linkage roller (40) is fixedly installed with a reciprocating screw (35), and the squeezing plate (34) is fixedly installed with a ball nut disc (36), and the ball nut disc (36) cooperates with the reciprocating screw (35).

2. The integrated sheet preparation and dyeing machine according to claim 1, characterized in that, The integrated box (1) is equipped with a control display screen (2), which is used to control the opening and closing and operation status of the transverse component, the feeding unit and the preparation unit, so as to realize the automated preparation of stained glass slides.

3. The integrated sheet preparation and dyeing machine according to claim 1, characterized in that, The supporting components include a slide preparation plate (7), a pipette holder (8), and a sample tray (27) set on the preparation platform (3). The slide preparation plate (7) is used to place multiple stained glass slides to be prepared, the pipette holder (8) is used to place clean pipette tips to be used, and the sample tray (27) is used to place multiple sample tubes to be sampled.

4. The integrated sheet preparation and dyeing machine according to claim 3, characterized in that, The transverse movement assembly includes a transverse moving stage (9) disposed in an integrated box (1), a longitudinal moving stage (10) disposed on the transverse moving stage (9), the transverse moving stage (9) being used to realize the transverse movement of the longitudinal moving stage (10), and a longitudinal sliding plate (12) disposed on the longitudinal moving stage (10), the longitudinal moving stage (10) being used to realize the longitudinal movement of the longitudinal sliding plate (12).

5. The slide preparation and dyeing integrated machine according to claim 4, characterized in that, The dyeing assembly includes two electric telescopic rods (22) fixedly installed inside the dyeing box (13). Each of the two electric telescopic rods (22) is fixedly equipped with a lifting frame (23), and both lifting frames (23) are slidably connected to the dyeing box (13). Each of the two lifting frames (23) is fixedly equipped with a lifting rod (15), and each of the two lifting rods (15) is equipped with two dyeing needle groups (16) for adding different dye solutions. The integrated box (1) is provided with a dyeing telescopic tube (5), and two dyeing injection tubes are provided inside the dyeing telescopic tube (5). The dyeing box (13) is provided with a connecting tube that is connected to the two dyeing injection tubes. Two dispensing tubes are fixedly connected to the connecting tube, and each dispensing tube is connected to the corresponding two dyeing needle groups (16).

6. The slide preparation and dyeing integrated machine according to claim 5, characterized in that, The sample loading assembly includes two electric telescopic rods (25) fixedly installed inside the sample loading box (14). The lower ends of the two electric telescopic rods (25) are fixedly installed with lifting frames (26). The two lifting frames (26) are fixedly installed with sample loading needles (17). The sample loading box (14) is provided with a sample loading cylinder (24). The sample loading cylinder (24) is fixedly connected with two telescopic conduits, and the two telescopic conduits are respectively connected to the two sample loading needles (17). The sample loading cylinder (24) and the integrated box (1) are fixedly connected with a sample loading telescopic tube (4).

7. The integrated sheet preparation and dyeing machine according to claim 6, characterized in that, The cleaning assembly includes a rinsing cylinder (18) fixedly installed on a longitudinal sliding plate (12), and a second water guide pipe frame (21) is fixedly connected between the rinsing cylinder (18) and the connecting pipe. A first water guide pipe frame (20) is fixedly connected between the rinsing cylinder (18) and the sample filling cylinder (24), and both the first water guide pipe frame (20) and the second water guide pipe frame (21) are equipped with control valves. A connecting water supply pipe (19) for water supply is fixedly connected to the lower part of the rinsing cylinder (18).

8. The integrated sheet preparation and dyeing machine according to claim 7, characterized in that, The centrifugation assembly includes a centrifuge box (6) fixedly installed on the preparation platform (3). A servo motor (32) is fixedly installed inside the centrifuge box (6), and a drive roller is fixedly installed on the drive end of the servo motor (32). A centrifuge rack (30) is fixedly installed on the drive roller, and multiple centrifuge boxes (31) are provided on the centrifuge rack (30). Multiple centrifuge tubes for placing samples are provided on the centrifuge boxes (31).

9. A slide preparation and dyeing integrated machine according to claim 8, characterized in that, Both water inlet pipes (29) are connected to the water supply pipe (19). Two waste liquid drain pipes are fixedly connected to the waste liquid tank (28), and the inlet ends of the two waste liquid drain pipes are located on the upper part of the sewage bearing plate (37).

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