Plant disease resistance related protein TaCNGC10 and coding gene and application thereof

Abstract

The invention belongs to the technical field of biology, and particularly relates to a plant disease resistance related protein TaCNGC10 as well as a coding gene and application thereof. The invention provides a plant disease resistance related protein TaCNGC10. The amino acid sequence of the plant disease resistance related protein TaCNGC10 is as shown in SEQ ID NO: 2. According to the invention, function analysis is carried out on related proteins and genes, so that a disease-resistant mechanism of the protein and the genes in interaction of plants and stripe rust is disclosed, and a solution is provided for cultivation of stripe rust-resistant materials.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of biotechnology, and particularly relates to a plant disease resistance related protein TaCNGC10, a coding gene thereof and application thereof. BACKGROUND

[0002] Puccinia is a obligate biotrophic fungus, its growth and reproduction completely depend on the host plant, water and nutrients must be obtained from the host plant. Different races of Puccinia have different effects on host plants. Because Puccinia uredospores can survive for a limited period of time, and the rest of the time needs to use the host plant to complete its life history. During the infection process, the host plant induces a defense response to prevent more toxic physiological races or other pathogenic microorganisms from infecting. However, the growth and development of the host plant also changes. When the defense response of the host plant is induced, the energy and related nutrient metabolism in the plant change, the uptake of nutrients and water is reduced, and the growth and development of the host plant is inhibited. When Puccinia invades the host plant, the host plant shows symptoms such as yellowing and necrosis, or when the fungus produces a large number of uredospore piles on the host surface, the green leaves and tissues are reduced, which greatly reduces the photosynthesis of the plant. Macroscopically, the infection of Puccinia can weaken the vitality of the host plant, reduce the number of tillers, panicles and seeds of the host plant, and can also reduce the plant height and the weight and quality of the seeds. Economically, the infection of Puccinia leads to a decrease in crop yield, and increases the management cost of crops. In agriculture, Puccinia is one of the diseases in wheat, barley and some pastures, which can cause disease outbreaks. For wheat, due to the outbreak of Puccinia, the yield loss can be 10-70% in general years, and even absolute yield loss in severe years. Planting disease-resistant varieties is considered to be an effective measure to control Puccinia.

[0003] In recent years, with the popularization of excellent varieties and the improvement of water and fertilizer conditions, the production level of wheat has been greatly improved. However, due to changes in climate and environment, unreasonable cultivation measures by humans, and variation of toxic races of Puccinia in wheat, the resistance of disease-resistant varieties may be lost, which may pose a potential safety hazard to wheat production. Using disease-resistant varieties for control is still an effective way at present. The use of plant disease resistance genes to inhibit the infection of plant pathogens and the increase of the content of plant disease resistance proteins will enhance the disease resistance of plants, and the expression of plant disease resistance genes will also enhance the disease resistance of plants.

[0004] Therefore, mining plant disease resistance genes, understanding the response and signal transduction mechanism of plants under Puccinia stress, and improving the disease resistance of plants have become important means for sustainable control of plant Puccinia. SUMMARY

[0005] The present invention uses genetic engineering to screen out the plant disease resistance-related protein TaCNGC10 and the plant disease resistance-related gene TaCNGC10 that encodes the plant disease resistance-related protein TaCNGC10. By functional analysis of the protein and gene, their disease resistance mechanism in the interaction between plants and stripe rust is revealed, providing a solution for the cultivation of stripe rust-resistant materials.

[0006] To achieve this technical purpose, the present invention adopts the following technical solutions.

[0007] In a first aspect, a plant disease resistance-related protein TaCNGC10 is provided, wherein the amino acid sequence of the plant disease resistance-related protein TaCNGC10 is: 1) as shown in SEQ ID NO: 2; or 2) A derivative amino acid sequence obtained by substituting, deleting and / or adding one or more amino acids from the amino acid sequence in 1), wherein the derivative amino acid sequence has the activity of the amino acid sequence shown in 1).

[0008] In the present invention, in order to facilitate the purification of the plant disease resistance-related protein TaCNGC10 in 1), a tag as shown in Table 1 can be connected to the amino terminus or carboxyl terminus of the protein consisting of the amino acid sequence shown in SEQ ID NO: 2.

[0009] In the present invention, the plant disease resistance-related protein TaCNGC10 in 2) can be artificially synthesized, or its encoding gene can be synthesized first and then expressed biologically. The encoding gene of the plant disease resistance-related protein TaCNGC10 in 2) can be obtained by deleting one or more amino acid residue codons from the plant disease resistance-related gene TaCNGC10 sequence shown in SEQ ID NO:1, and / or performing one or more base pair missense mutations, and / or attaching the coding sequence of the tag shown in Table 1 to its 5' and / or 3' ends.

[0010] Table 1 Tag sequences

[0011] In a second aspect, a plant disease resistance-related gene TaCNGC10 is provided, wherein the plant disease resistance-related gene TaCNGC10: a) encoding the plant disease resistance-related protein TaCNGC10 of the present invention; b) the CDS sequence is shown in SEQ ID NO: 1; or c) is complementary to a) or b).

[0012] In the present application, the plant disease resistance related gene TaCNGC10 sequence has at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% homology and encodes a DNA molecule with the same functional protein.

[0013] In the present application, the plant disease resistance related gene TaCNGC10 encoding the plant disease resistance related protein TaCNGC10 of the present application can be easily mutated by the person skilled in the art using known methods, such as directed evolution and point mutation. Those artificially modified polynucleotides with 75% or higher homology to the plant disease resistance related gene TaCNGC10 sequence of the plant disease resistance related protein TaCNGC10 isolated from the present application are derived from the plant disease resistance related gene TaCNGC10 sequence of the present application and are equivalent to the sequence of the present application as long as they encode the plant disease resistance related protein TaCNGC10 and have the same function.

[0014] In the present application, the term "homology" refers to sequence similarity to the natural nucleic acid sequence. "Homology" includes nucleotide sequences with 75% or higher, or 85% or higher, or 90% or higher, or 95% or higher homology to the polynucleotide sequence of the present application encoding the protein consisting of the amino acid sequence shown in SEQ ID NO: 2. Homology can be evaluated by the naked eye or computer software. Using computer software, the homology between two or more sequences can be expressed as a percentage (%), which can be used to evaluate the homology between related sequences. Among them, 75% or higher homology can be 80%, 85%, 90% or 95% or higher homology.

[0015] In a third aspect, a polynucleotide construct or host cell containing the plant disease resistance related gene TaCNGC10 as described in the present application is provided.

[0016] In a fourth aspect, a recombinant vector, expression cassette or recombinant bacteria containing the plant disease resistance related gene TaCNGC10 as described in the present application is provided.

[0017] In the present application, the expression cassette refers to a DNA capable of expressing a plant disease resistance-related gene TaCNGC10 as shown in SEQ ID NO: 1 in a host cell, which can include not only a promoter that initiates transcription of a polynucleotide as shown in SEQ ID NO: 1, but also a terminator that terminates transcription of a plant disease resistance-related gene TaCNGC10 as shown in SEQ ID NO: 1. Further, the expression cassette can further include an enhancer sequence. The promoters that can be used in the present application include, but are not limited to, constitutive promoters, tissue-, organ-, and development-specific promoters, and inducible promoters. Suitable transcription terminators include, but are not limited to, Agrobacterium nopaline synthase terminator (NOS terminator), CaMV 35S terminator of cauliflower mosaic virus, tml terminator, pea rbcS E9 terminator, and opine and octopine synthase terminators.

[0018] In the present application, a recombinant vector containing the expression cassette can be constructed using an existing expression vector. The plant expression vector includes a vector that can be used for plant microprojectile bombardment, etc. Such as pAHC25, pBin438, pCAMBIA1302, pCAMBIA2300, pCAMBIA2301, pCAMBIA1305, pCAMBIA1300, pBI121, pCAMBIA1391-Xa or pCAMBIA1391-Xb (CAMBIA), etc. The plant expression vector can further include a 3 ’ untranslated region of an exogenous gene, i.e., a DNA fragment including a polyadenylation signal and any other DNA fragment involved in mRNA processing or gene expression. The polyadenylation signal can direct the addition of polyadenosine to the 3 ’ end of the mRNA precursor, such as Agrobacterium crown gall tumor-inducing (Ti) plasmid genes (such as nopaline synthase gene Nos), plant genes (such as soybean storage protein gene) 3 ’The untranslated regions of the 5' and 3' ends of the transcription have similar functions. When the gene construction plant expression vector of the present application is used, enhancers, including translation enhancers or transcription enhancers, can also be used. These enhancer regions can be ATG start codon or the adjacent region start codon, etc., but must be in the reading frame of the coding sequence to ensure the correct translation of the entire sequence. The source of the translation control signal and the start codon is wide, which can be natural or synthetic. The translation initiation region can be from the transcription initiation region or the structural gene. In order to facilitate the identification and screening of transgenic plant cells or plants, the plant expression vector used can be processed, such as adding genes that can express enzymes or luminescent compounds that can produce color changes in plants (GUS gene, luciferase gene, etc.), marker genes of antibiotics (such as nptII gene conferring resistance to kanamycin and related antibiotics, bar gene conferring resistance to herbicide phosphine silk fungus, hph gene conferring resistance to antibiotic hygromycin, and dhfr gene conferring resistance to aminomethylptero, EPSPS gene conferring resistance to glyphosate), or chemical reagent resistance marker genes (such as herbicide resistance genes), mannose-6-phosphate isomerase genes that provide the ability to metabolize mannose. For the safety of transgenic plants, no selective marker gene can be added, and the transformed plants can be directly screened under stress.

[0019] In the present application, the vector can be a plasmid, a cosmid, a bacteriophage or a viral vector.

[0020] In the present application, the microorganism can be yeast, bacteria, algae or fungi, such as Agrobacterium.

[0021] In the present application, the transgenic plant cell lines do not include reproductive materials.

[0022] In a fifth aspect, the polynucleotide construct or host cell of the present application is provided for use in regulating the disease resistance of a plant.

[0023] Preferably, the disease resistance is resistance to stripe rust.

[0024]

[0024] In a sixth aspect, the polynucleotide construct or host cell of the present application is provided for use in cultivating a disease-resistant plant.

[0025] Preferably, the disease resistance is resistance to stripe rust.

[0026] In a seventh aspect, the recombinant vector, expression cassette or recombinant bacteria of the present application are provided for use in regulating the disease resistance of a plant.

[0027] Preferably, the disease resistance is resistance to stripe rust.

[0028] In an eighth aspect, the recombinant vector, expression cassette or recombinant bacteria of the present application are provided for use in cultivating a disease-resistant plant.

[0029] Preferably, the disease resistance is resistance to stripe rust.

[0030] In a ninth aspect, a method for cultivating a transgenic plant with improved disease resistance is provided, the method comprising the following steps: 1) increasing the content and / or activity of the plant disease resistance related protein TaCNGC10 of the present application in a target plant to obtain a transgenic plant; 2) increasing the expression of the plant disease resistance related gene TaCNGC10 encoding the plant disease resistance related protein TaCNGC10 of the present application in a target plant to obtain a transgenic plant; wherein the disease resistance of the transgenic plant is higher than that of the target plant.

[0031] Preferably, the increase of the content and / or activity of the plant disease resistance related protein TaCNGC10 of the present application in a target plant is achieved by introducing the plant disease resistance related gene TaCNGC10 of the present application into the target plant.

[0032] Preferably, the increase of the expression of the plant disease resistance related gene TaCNGC10 encoding the plant disease resistance related protein TaCNGC10 of the present application in a target plant is achieved by introducing the plant disease resistance related gene TaCNGC10 of the present application into the target plant.

[0033] In the present application, the improvement of the plant resistance to stripe rust is embodied in any one of the following (1)-(3): (1) under the stress of Puccinia striiformis, the spore production of Puccinia striiformis invading the transgenic wheat is lower than that of the recipient plant; (2) under the stress of Puccinia striiformis, the expression level of the disease-related genes of the transgenic plant is higher than that of the recipient plant; (3) under the stress of Puccinia striiformis, the mycelial invasion area of Puccinia striiformis invading the transgenic wheat is lower than that of the recipient plant. The stress of Puccinia striiformis is affinity treatment, and the affinity treatment uses CYR32 and CYR34 strains.

[0034] In the present application, wheat is selected as the research object because Puccinia striiformis is a specific parasitic fungus. Fielder is used as the recipient and research object of transgenesis.

[0035] In the present application, the coding gene of the plant disease resistance related protein TaCNGC10 (i.e. the nucleotides shown in SEQ ID NO: 1 from 5' 1-2085) is introduced into Agrobacterium EHA105 through a recombinant vector CUB-TaCNGC10 containing an expression cassette of the coding gene of TaCNGC10 protein. The recombinant vector CUB-TaCNGC10 is obtained by inserting the DNA fragment of TaCNGC10 into the CUB vector by homologous recombination, and the other sequences of the CUB vector remain unchanged. The vector enzyme digestion site is BamH1.

[0036] In the present invention, transgenic plants are understood to include not only first-generation transgenic plants obtained by transforming a recipient plant with the plant disease resistance-related gene TaCNGC10, but also their progeny. Transgenic plants can be propagated within the species in which they are grown, or they can be transferred into other varieties of the same species, particularly commercial varieties, using conventional breeding techniques. Transgenic plants include seeds, callus, whole plants, and cells.

[0037] Compared with the prior art, the present invention has the following beneficial effects: The plant disease resistance gene TaCNGC10, verified in this invention, is induced to express by stripe rust. Transgenic wheat obtained by introducing TaCNGC10 into wheat exhibits greater resistance to multiple physiological races of stripe rust than wild-type wheat. The proteins and genes provided by this invention provide a foundation for artificially controlling the expression of disease resistance genes and will play an important role in cultivating plants with broad-spectrum disease resistance.

[0038] This study clarifies the role of the plant disease resistance gene TaCNGC10 in the interaction between wheat and stripe rust. Experiments have demonstrated that the plant disease resistance gene TaCNGC10 can be used to improve wheat stripe rust resistance. The plant disease resistance gene TaCNGC10 provided by this invention provides a genetic resource for breeding stripe rust-resistant wheat. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] In order to more clearly illustrate the technical solution of the present invention, the following is a brief introduction to the drawings involved in the description of the embodiments. Obviously, the drawings in the description are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0040] Figure 1 TaCNGC10 (a plant disease resistance-related gene TaCNGC10 ) in the interaction combination of wheat and stripe rust.

[0041] Figure 2 TaCNGC10 (a plant disease resistance-related gene TaCNGC10 ) PCR identification results of overexpressing wheat T3 generation.

[0042] Figure 3 TaCNGC10 (a plant disease resistance-related gene TaCNGC10 ) Identification of disease resistance of genetically modified wheat. DETAILED DESCRIPTION

[0043] The technical solutions of the present invention are described below with reference to the following examples. However, the present invention is not limited to the following examples. The experimental methods and detection methods described in each example are conventional methods unless otherwise specified; the reagents and materials described are commercially available unless otherwise specified.

[0044] The wheat stripe rust race CYR23 in the embodiments of the present invention is disclosed in the document "Liu P, Guo J, Zhang R, et al. TaCIPK10 interacts with and phosphorylates TaNH2 to activate wheat defense responses to stripe rust [J]. Plant biotechnology journal, 2019, 17(5): 956-968." The public can obtain it from the applicant.

[0045] The wheat stripe rust race CYR31 in the embodiment of the present invention has been disclosed in the document "Wang Fengle, Wu Liren, Xu Shichang, Jin Shelin, Jia Qiuzhen, Yuan Wenhuan, Yang Jiaxiu. Research on new stripe rust races CYR30 and CYR31 in China [J]. Acta Phytophylacica Sinica, 1996(01):39-44." The public can obtain it from the applicant.

[0046] The wheat variety Shuiyuan 11 in the embodiment of the present invention is disclosed in the document "Cao Zhangjun, Jing Jinxue, Wang Meinan, et al. Analysis of the relationship between stripe rust resistance genes of important domestic resistant varieties Shuiyuan 11, Shuiyuan 92 and Hybrid 46 [J]. Journal of Northwest Botany, 2003, 23(1): 64-68." The public can obtain it from the applicant.

[0047] Example 1 This example provides the plant disease resistance-related protein TaCNGC10 (TaCNGC10) and its encoding gene. 1. Isolation of mRNA and plant disease resistance-related gene TaCNGC10 ( TaCNGC10 ) Normally grown 7-day-old wheat seedlings of the Shuiyuan 11 variety were taken, quickly frozen with liquid nitrogen, and stored at -80°C for later use.

[0048] Total RNA was extracted from wheat leaves using the Polysaccharide and Polyphenol Plant RNA Extraction Kit (Huayueyang Biotechnology Co., Ltd.). First-strand cDNA was synthesized using Reverse Transcriptase XL (AMV). cDNA was synthesized using the SMART method, and PCR products were analyzed by 1.0% agarose gel electrophoresis. Amplification primers were: TaCNGC10 -F:5 ’-ATGTTCGCGCCGAGGAAGGTGGAGG-3 ’ ; TaCNGC10 -R:5 ’ -CTCGTAGGGTTCGACCGAGAAATCT-3 ’ .

[0049] A 2085 bp PCR product was obtained. After sequencing, the PCR product contained the nucleotides shown in positions 1 to 2085 of SEQ ID NO: 1, and the gene containing the nucleotides was named plant disease resistance-related gene TaCNGC10 ( TaCNGC10 ); TaCNGC10 The amino acid sequence of the encoded protein is shown in SEQ ID NO: 2, and the protein is named plant disease resistance-related protein TaCNGC10 (TaCNGC10).

[0050] 2. RT-PCR Testing TaCNGC10 Expression induced by stripe rust 1. Preparation of experimental materials Stripe rust inoculation was performed according to the method described by Kang Zhensheng et al. (1984, Journal of Northwest Agricultural University). Leaves of the wheat variety Shuiyuan 11 were inoculated with either CYR23 (non-compatible) or CYR31 (compatible) to form a non-compatible and compatible interaction. Sterile water served as a control.

[0051] Samples were collected at 0, 6, 12, 24, 48, 72, 96, and 120 hours after inoculation. Control samples were collected at the same time points as the treatments. Fresh leaves were cut, wrapped in tin foil, and quickly frozen in liquid nitrogen before being stored at -80°C. Total RNA from wheat leaves was extracted using the Trizol method, and first-strand cDNA was synthesized using Reverse Transcriptase XL (AMV). cDNA was synthesized using the SMART method.

[0052] 2. RT-qPCR detection TaCNGC10 The expression level According to wheat TaCNGC10 and elongation factor genes TaEF Specific quantitative PCR primers were designed based on the sequence of the gene (GenBank accession number: U76744).

[0053] The RT-qPCR primer sequences are: TaCNGC10 -RT-F: 5 ’ -GGTGAAGATGACCCTCCACCCAAG-3 ’ ; TaCNGC10- F: 5'-CTCGTAGGGTTCGACCGAGAAATCT-3 ’ ; TaEF - F: 5 ’ - F: 5'-CTCGTAGGGTTCGACCGAGAAATCT-3 ’ ; TaEF - F: 5 ’ - F: 5'-CTCGTAGGGTTCGACCGAGAAATCT-3 ’ .

[0054] The specificity and amplification efficiency (≥90%) of the quantitative PCR primers were detected before use. TaEF-1a was used as an internal reference gene in the Real-time PCR analysis. AceQ Universal SYBR qPCR Master Mix (Vazyme, Nanjing, China) and Bio-Rad CFX Manager quantitative PCR instrument (Bio-rad, Hercules, California) were used to perform real-time quantitative PCR amplification according to the instructions, and the cDNA of each treatment sampling point was used as the template. Each reaction was repeated at least three times, and the Ct value, its average value and standard deviation of each repeat were generated by manual adjustment of the baseline by the quantitative PCR instrument. Each reaction was repeated three times, and the Ct value was averaged. The experimental data were analyzed by the Delta Delta Ct method to determine the relative expression of the gene.

[0055] The results of RT-qPCR are shown in Figure 1 , TaCNGC10 The expression patterns of the wheat variety Shuiyuan 11 after inoculation with the non-compatible race CYR23 and the compatible race CYR31 of Puccinia striiformis, wherein TaCNGC10 The expression was up-regulated in the pre-infection stage after inoculation in the non-compatible combination and the compatible combination.

[0056] The above results show that, TaCNGC10 The expression is induced by Puccinia striiformis.

[0057] Example 2 This example provides TaCNGC10 application in improving plant resistance to stripe rust One, TaCNGC10 Obtaining of transgenic wheat 1、 TaCNGC10 Construction of overexpression vector The amplified TaCNGC10 from Example 1 ’The 1-2085 nucleotide fragment was homologously recombined with the CUB vector after BamH I digestion using the ClonExpress II OneStep Cloning Kit (vazyme) to obtain the recombinant vector. TaCNGC10 -CUB.

[0058] TaCNGC10 The specific construction method of -CUB vector is as follows: Amplified TaCNGC10 The full-length CDS was used as a template and amplified with the following primers. The resulting fragment was directly homologously recombined into the CUB vector.

[0059] TaCNGC10 -CUB-F: 5 ’ -CAGGTCGACTCTAGA GGATCC ATGTTCGCGCCGAGGAAGGTGGAGG-3 ’ ; TaCNGC10 -CUB-R: 5 ’ -GAGCTCGGTACCCGG GGATCC CTCGTAGGGTTCGACCGAGAAATCT-3 ’ ; Used for TaCNGC10 For transgenic wheat transformation, the restriction enzyme cutting site is BamH I. The underline in the above primers indicates the restriction enzyme cutting site.

[0060] 2. TaCNGC10 Preparation of transgenic wheat Recombinant vector TaCNGC10 -CUB used Agrobacterium to infect wild-type wheat Fielder callus and obtained T0 generation transgenic TaCNGC10 Genetically modified wheat.

[0061] Cultivate T0 generation TaCNGC10 Genetically modified wheat until T3 generation TaCNGC10 Two lines of genetically modified wheat.

[0062] 3. PCR verification T3 transgenic plants were extracted using CTAB method. TaCNGC10 The genomic DNA of leaves of two transgenic wheat lines and wild-type control wheat (Fielder) was used TaCNGC10 Overexpression detection F: 5 ’ -CATCGTCAACTTCCTGATGAATTGC-3 ’ BESNOS2-R: 5 ’-TTGCGGGACTCTAATCATAAAAAC-3 ’ Molecular testing was performed on the transgenic T3 plants (upstream primers were located on the gene, and downstream primers were located on the NOS terminator). Five plants were randomly selected from each line. Water was used as a blank control, and DL2000 Plus DNA Marker (vazyme) was used as a standard. The standard indicated a size of Figure 2 shown.

[0063] The results are as follows Figure 2 As shown, the positive strain obtained was 846 bp, which was overexpressed TaCNGC10 The T3 generation of two lines ( TaCNGC10 Overexpression lines 13 and TaCNGC10 Overexpression lines 46) were all positive lines and were named TaCNGC10 Overexpression strain 13, TaCNGC10 Overexpression line 46.

[0064] 2. Transfer TaCNGC10 Analysis of stripe rust resistance in genetically modified wheat Wild wheat Fielder (materials from the College of Plant Protection, Northwest Agriculture and Forestry University), TaCNGC10 Overexpression lines 13 and TaCNGC10 Overexpression line 46 was grown in an incubator with a day / night temperature of 25 / 23°C and a photoperiod of 16 h light / 8 h dark after the second leaf expanded ( Figure 3 (A) Stripe rust races CYR32 and CYR34 (from the College of Plant Protection, Northwest Agriculture and Forestry University) were inoculated, respectively. The inoculation method was based on the literature “Kang Zhensheng, Li Zhenqi. Discovery of a new pathogenic strain of Lovelin 10 at room temperature [J]. Journal of Northwest Agriculture and Forestry University (Natural Science Edition) 1984(04):18-28.” RNA samples were extracted from leaves at the inoculation site 120 h after inoculation, and the disease phenotype was observed 14 days after inoculation.

[0065] Phenotypic results such as Figure 3 As shown in Figure A, under the infection conditions of stripe rust fungi CYR32 and CYR34, a large number of spore piles were observed on the leaves of all treatments of the control plants, while TaCNGC10 Overexpression strain 13, TaCNGC10 The number of summer spores in wheat leaves of overexpression line 46 was significantly reduced, and the area of ​​chlorosis and necrosis increased. TaCNGC10 Overexpression lines 13 and TaCNGC10 Overexpression line 46 TaCNGC10 The expression levels of 120 h after different inoculations were detected by RT-PCR according to the method in Example 1. Figure 3 As shown in B, the overexpression materialTaCNGC10 The expression amount is always more than 5 times higher than the control.

[0066] III. Biomass detection The biomass of stripe rust in wheat 7 days after inoculation was detected. The detection method of biomass refers to the literature "Qi Tuo et al., Stripe Rust Effector PstGSRE1 Disrupts Nuclear Localization of ROS-Promoting Transcription Factor TaLOL2 to Defeat ROS-Induced Defense in Wheat, 2019". The results are shown in Table 2. Figure 3 As shown in Table 2, it is found that the biomass of stripe rust in wheat is significantly reduced by overexpression.

[0067] The above disease resistance identification results show that under the conditions of CYR32 and CYR34 two physiological races, the transgenic wheat shows strong resistance. This has great advantages in production application.

[0068] Therefore, it is proved that TaCNGC10 is an important gene involved in the process of wheat resistance to stripe rust.

[0069] The above-described examples are part of the embodiments of the present application, rather than all the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the claimed application, but to represent selected embodiments of the application. All other embodiments obtained by related deduction and replacement made by those skilled in the art under the condition of the concept of the present application, without making creative labor, belong to the scope of protection of the present application.

Claims

1. A plant disease resistance-related protein TaCNGC10, characterized in that: The amino acid sequence of the plant disease resistance-related protein TaCNGC10 is shown in SEQ ID NO:

2.

2. A plant disease resistance-related gene TaCNGC10, characterized in that: The plant disease resistance-related gene TaCNGC10: a) encoding the plant disease resistance-related protein TaCNGC10 according to claim 1; b) the CDS sequence is shown in SEQ ID NO: 1; or c) is complementary to a) or b).

3. A polynucleotide construct or host cell containing the plant disease resistance-related gene TaCNGC10 as claimed in claim 2.

4. A recombinant vector, expression cassette or recombinant bacterium containing the plant disease resistance-related gene TaCNGC10 as claimed in claim 2.

5. Use of the polynucleotide construct or host cell according to claim 3 in regulating plant disease resistance.

6. Use of the polynucleotide construct or host cell according to claim 3 in cultivating disease-resistant plants.

7. Use of the recombinant vector, expression cassette or recombinant bacterium according to claim 4 in regulating plant disease resistance.

8. Use of the recombinant vector, expression cassette or recombinant bacterium according to claim 4 in cultivating disease-resistant plants.

9. The use according to any one of claims 5 to 8, characterized in that: The disease resistance is resistance to stripe rust.

10. A method for cultivating transgenic plants with improved disease resistance, characterized in that: The method comprises the following steps: 1) increasing the content and / or activity of the plant disease resistance-related protein TaCNGC10 according to claim 1 in a target plant to obtain a transgenic plant; 2) increasing the expression of the plant disease resistance-related gene TaCNGC10 encoding the plant disease resistance-related protein TaCNGC10 according to claim 1 in the target plant to obtain a transgenic plant; The disease resistance of the transgenic plant is higher than that of the target plant.

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