Sample pretreatment device of flow cytometer

Abstract

The invention discloses a flow cytometer sample pretreatment device, and belongs to the technical field of cell pretreatment, the flow cytometer sample pretreatment device comprises a base, the top end of the base is fixedly connected with a treatment box, the top end of the treatment box is slidably connected with a capping assembly, and the inner side of the treatment box is rotatably connected with a pretreatment assembly. In the pretreatment process, the test tube fixing frame realizes stable fixation and flexible adjustment of a test tube through the combination of the convex column, the double-layer sliding frame, the fan-shaped frame and other components. Meanwhile, the electric motor drives the test tube fixing frame to shake through the transmission belt, so that the cells are broken, and subsequent centrifugal treatment is facilitated. The design of the hydraulic push rod provides power support in the vertical direction for the whole device, adjustment of the deflection angle is achieved, the motor serves as a power source, the whole pretreatment assembly is driven to rotate by driving the installation column to rotate, uniform pretreatment of samples in test tubes is achieved, and the centrifugal effect is further improved.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of cell pretreatment, and particularly relates to a flow cytometer sample pretreatment device. BACKGROUND

[0002] As an indispensable key equipment in precision medical diagnosis, the flow cytometer has been included in high-end diagnosis and treatment guidelines such as blood tumor typing, immune cell function evaluation, and circulating tumor cell counting, and gradually penetrated into high-end physical examination and scientific research health management centers, because it can realize multi-parameter quantitative analysis at the single cell level. The flow cytometer needs to pretreat the detection cells before use, thereby improving the detection effect and efficiency.

[0003] However, the flow cytometer sample pretreatment process is complicated, needs manual operation of multiple steps, and needs multiple devices for processing, which is low in efficiency and easy to be affected by human factors, resulting in poor sample processing effect and affecting the subsequent detection and analysis results. SUMMARY

[0004] The application aims to overcome the shortcomings of the prior art and provide a flow cytometer sample pretreatment device.

[0005] The technical scheme adopted to solve the above technical problems is a flow cytometer sample pretreatment device, which comprises a base, a treatment box fixedly connected to the top end of the base, a top sealing assembly slidingly connected to the top end of the treatment box, and a pretreatment assembly rotatably connected to the inner side of the treatment box.

[0006] Further, the pretreatment assembly comprises a connecting plate, an installation column fixedly connected to the bottom end of the connecting plate, the installation column being rotatably connected to the base, two fan-shaped frames fixedly connected to the two ends of the connecting plate, two double-layer carriages slidingly connected to the inner sides of the two fan-shaped frames, a connecting frame fixedly connected to the middle of the installation column, test tube fixing frames rotatably connected to the two sides of the connecting frame, and convex columns fixedly connected to the two ends of the test tube fixing frames.

[0007] Through the above technical scheme, the test tube fixing frame can stably fix the test tube, and the design of the convex column prevents the test tube from falling off during rotation. The design of the double-layer carriage enables the test tube to switch between different steps, realizes shaking and centrifugation effects, increases the flexibility of the device, and the combination of the connecting plate, the installation column and the connecting frame enables the pretreatment assembly to rotate around the base, thereby realizing uniform pretreatment of the sample in the test tube. During the pretreatment process, the positions of the fan-shaped frame and the double-layer carriage, and the angle of the test tube fixing frame can be adjusted to adapt to test tubes of different sizes and shapes, thereby improving the applicability and practicability of the device.

[0008] Further, the middle of the fan-shaped frame is provided with a plurality of curved grooves, the curved grooves are in sliding connection with the convex column, the convex column penetrates one of the layers between the double-layer slide, the double-layer slide is in sliding connection with the top column in the middle, the top column is in sliding contact with the convex column at the top end, the second sliding groove is fixedly connected to the middle of the top column, the convex circular plate is in sliding connection with the inner side of the second sliding groove, the second sliding groove is in rotary connection with the double-layer slide, the single-head reel is fixedly connected to the middle of the convex circular plate, the inner side of the double-layer slide is fixedly connected with the electric motor, the double-head reel is fixedly connected to the power output end of the electric motor, and the transmission belt is in friction transmission connection between the double-head reel and the single-head reel.

[0009] Through the above technical scheme, the sliding connection design of the curved groove and the convex column makes the movement of the test tube fixing frame on the double-layer slide more stable, and the design of the top column further enhances the stability of the test tube fixing frame. The sliding contact of the top column with the convex column and the rotary connection of the second sliding groove with the double-layer slide enable the convex circular plate and the single-head reel to rotate flexibly. The electric motor drives the transmission belt to operate through the friction transmission connection between the double-head reel and the single-head reel, so as to shake the sample in the test tube, break the cells, and facilitate subsequent centrifugal processing. This design not only improves the efficiency of sample pretreatment, but also makes the entire pretreatment process more automated and intelligent, reducing the difficulty and intensity of manual operation.

[0010] Further, the middle of the double-layer slide is fixedly connected with a hydraulic push rod, the bottom end of the hydraulic push rod is fixedly connected with the fan-shaped frame, and the extension end of the hydraulic push rod is fixedly connected with the double-layer slide.

[0011] Through the above technical scheme, the design of the hydraulic push rod provides vertical power support for the entire device. When the extension end of the hydraulic push rod works, it can push the fan-shaped frame to move in the direction of the curved groove. This movement adjusts the offset angle of the test tube fixing frame, further improves the centrifugal effect, and the fixed connection of the hydraulic push rod with the double-layer slide ensures the stability and reliability of the entire device during operation, avoiding shaking or deviation caused by shaking, thereby ensuring the accuracy and reliability of the pretreatment results.

[0012] Further, the bottom end of the fan-shaped frame is fixedly connected with a limiting boss, the limiting boss is in sliding connection with the base, a plurality of elastic push columns are rotatably connected to the middle of the mounting column, and the extension end of the elastic push column is rotatably connected to the middle of the test tube fixing frame.

[0013] Through the technical scheme, the design of the limiting boss further enhances the stability of the fan-shaped frame in the operation process, and through the sliding connection with the base, the fan-shaped frame can maintain a stable movement track when adjusting the offset angle. The several elastic push columns rotatably connected in the middle part of the mounting column play a supporting and fine-tuning role on the test tube fixing frame. When the hydraulic push rod pushes the test tube fixing frame through the double-layer slide, the extension end of the elastic push column will adjust the pressure and angle of the test tube fixing frame as needed. This flexible supporting mode not only ensures the stability of the test tube during pretreatment, but also adjusts the inclination angle of the test tube according to actual needs, further improves the centrifugal effect, and at the same time, the rotary connection mode of the elastic push column makes the whole device more flexible and durable during operation, prolonging the service life of the device.

[0014] Further, the test tube fixing frame placing opening is fixedly connected with a U-shaped plate on the inner side, a pressure table is slidably connected through the bottom end of the U-shaped plate, fourth pull plates are rotatably connected on both sides of the bottom end of the pressure table, L-shaped push plates are rotatably connected on the ends of the fourth pull plates away from the pressure table, the L-shaped push plates are rotatably connected with the U-shaped plate in the middle, and clamping plates are fixedly connected on the ends of the L-shaped push plates away from the fourth pull plates.

[0015] Through the above technical scheme, when it is necessary to fix the test tube, the height of the pressure table is adjusted, the fourth pull plate is rotated, the L-shaped push plate is moved, and finally the clamping plate clamps and fixes the test tube. This design not only improves the stability and reliability of the test tube fixing, but also makes the operation process more simple and convenient. At the same time, since the rotary connection mode is adopted between each component, the whole fixing mechanism is more flexible during operation, can adapt to test tubes of different sizes and shapes, and further improves the practicality and applicability of the device.

[0016] Further, the bottom end of the base is fixedly connected with shock pads at four corner positions, the top end of the base is fixedly connected with a first sliding groove in the middle, the top end of the first sliding groove is rotatably connected with the mounting column, the base is provided with a support column at the top end, the support column is located outside the first sliding groove, the inner side of the support column is rotatably connected with the limiting boss, the top end of the base is rotatably connected with a plurality of first pull rods on both sides, the middle part of the base is fixedly connected with a motor, and the power output end of the motor is fixedly connected with the mounting column.

[0017] By the above technical scheme, when the device is working, the shock pad can effectively absorb and disperse the vibration generated by the device, ensuring the stability of the entire device during the centrifugation process, avoiding problems such as test tube breakage or sample spatter caused by vibration. The rotating connection design of the first sliding groove and the mounting column allows the pretreatment assembly to rotate around the base, increasing the flexibility of the device. The meshing sliding connection of the support column and the limiting boss further enhances the stability of the fan-shaped frame during rotation, avoiding shaking or deviation caused by rotation. The motor serves as a power source, driving the rotation of the mounting column and thus the entire pretreatment assembly, achieving uniform pretreatment of the sample in the test tube.

[0018] Further, a second pull rod is rotatably connected to the bottom end of each of the first pull rods, and a turntable is rotatably connected between two second pull rods on the same side. The middle of the turntable is rotatably connected to the inner wall of the base, and an electric push rod is rotatably connected to the surface of the side of the turntable away from the inner wall of the base. The extension end of the electric push rod is rotatably connected to the turntable, and the end of the electric push rod away from the turntable is rotatably connected to the base.

[0019] Further, a hollow area is provided on the inner side of the treatment box, and the hollow area is slidably connected to the fan-shaped frame. Limiting sliders are fixedly connected to the four corner positions at the top end of the treatment box, and the limiting sliders are slidably connected to the capping assembly. Sealing strips are fixedly connected to the two sides of the top end of the treatment box, and first missing slots are provided on the two sides of the treatment box, and the first missing slots are slidably fitted with the first pull rods.

[0020] By the above technical scheme, the design of the treatment box not only provides the necessary operation space for the pretreatment assembly, but also realizes the limiting and guiding of the pretreatment assembly in the vertical direction through the sliding connection of the hollow area and the fan-shaped frame, ensuring the stability and accuracy of the pretreatment assembly during rotation. The sliding connection of the limiting sliders and the capping assembly allows the capping assembly to be opened or closed flexibly, facilitating the placement and removal of samples. At the same time, this design also enhances the sealing performance of the treatment box, avoiding contamination or leakage of the sample during pretreatment. The sealing strips further improve the sealing effect of the treatment box, ensuring the cleanliness and safety of the pretreatment environment. The sliding fitting design of the first missing slots and the first pull rods not only provides the necessary movement trajectory for the first pull rods, but also makes the entire device more compact and reasonable in structure, avoiding the problems of complex operation or space waste caused by excessive components.

[0021] Furthermore, the capping assembly includes two empty boxes, which are right-angled trapezoidal structures. The short right-angled end of the empty box is slidably connected to a slide door, the inside of the empty box is fixedly connected to a test tube rack, a ventilation fan is installed on one side of the empty box, and second notches are provided on both sides of the long right-angled end of the empty box. The inside of the second notch is rotatably connected to a third pull rod, the third pull rod is rotatably connected to the first pull rod, and a limiting guide rail is provided on one side of the second notch, and the inner side of the limiting guide rail is engaged and slidably connected with the limiting slider.

[0022] Through the above technical solution, the design of the capping assembly cleverly combines practicality and convenience. The two empty boxes with right-angled trapezoidal structures not only provide stable storage space for test tubes, but also use sliding doors connected by short right-angle ends to easily open and close the empty boxes, facilitating the insertion and removal of test tubes. The provision of the test tube rack ensures the stability and safety of the test tubes during the pretreatment process, avoiding sample loss or contamination caused by test tube shaking. The installation of the ventilation fan effectively promotes air circulation within the empty box, helping to maintain a constant temperature and humidity in the pretreatment environment, thereby improving the pretreatment effect.

[0023] The beneficial effects of the present invention are as follows: 1. This invention achieves automated and intelligent flow cytometer sample preprocessing through a unique structural design, significantly improving preprocessing efficiency and accuracy. Specifically, the sliding connection between the processing chamber and the capping assembly, as well as the rotating design of the preprocessing assembly, provide a stable and flexible preprocessing environment for samples within the test tube.

[0024] 2. During the pretreatment process, the present invention utilizes a test tube holder, a combination of raised columns, a double-layer slide, and a fan-shaped frame, to achieve stable fixation and flexible adjustment of test tubes. Simultaneously, an electric motor, via a transmission belt, drives the test tube holder to vibrate, disrupting cells and facilitating subsequent centrifugation. The hydraulic push rod design provides vertical power support for the entire device, enabling adjustment of the deflection angle. The motor, serving as the power source, drives the rotation of the mounting column, thereby driving the rotation of the entire pretreatment assembly, achieving uniform pretreatment of the sample within the test tube and further improving the centrifugation effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a perspective schematic diagram of a first structure of the present invention; Figure 2 It is a schematic perspective view of a second structure of the present invention; Figure 3 It is a three-dimensional schematic diagram of the base structure of the present invention; Figure 4 It is a three-dimensional schematic diagram of the processing box structure of the present invention; Figure 5is the first perspective view schematic diagram of the empty box structure of the present application; Figure 6 is the second perspective view schematic diagram of the empty box structure of the present application; Figure 7 is the first cross-sectional view schematic diagram of the overall structure of the present application; Figure 8 is the second cross-sectional view schematic diagram of the overall structure of the present application; Figure 9 is the three-dimensional schematic diagram of the pretreatment assembly structure of the present application; Figure 10 is the first three-dimensional schematic diagram of the test tube fixing rack structure of the present application; Figure 11 is the front view schematic diagram of the pretreatment assembly structure of the present application; Figure 12 is the second three-dimensional schematic diagram of the test tube fixing rack structure of the present application.

[0026] The figure mark: 1, base; 101, shock pad; 102, support column; 103, first sliding groove; 104, first pull rod; 105, second pull rod; 106, turntable; 107, electric push rod; 108, motor; 2, processing box; 201, hollow area; 202, limit sliding block; 203, sealing strip; 204, first missing slot; 3, top sealing assembly; 301, empty box; 302, sliding plate door; 303, test tube placing rack; 304, ventilation fan; 305, second missing slot; 306, third pull rod; 307, limit guide rail; 4, pretreatment assembly; 401, connecting plate; 402, mounting column; 403, connecting frame; 404, test tube fixing rack; 405, fan-shaped frame; 406, double-layer sliding frame; 407, bent groove; 408, convex column; 409, elastic push column; 410, top column; 411, boss round plate; 412, electric motor; 413, double-head reel; 414, second sliding groove; 415, single-head reel; 416, transmission belt; 417, hydraulic push rod; 418, limit boss; 419, U-shaped plate; 420, pressing table; 421, fourth pull plate; 422, clamping plate; 423, L-shaped push plate. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical scheme and advantages of the present application more clear, the present application is further described in detail below in combination with the drawings and examples. It should be understood that the specific examples described herein are only used to explain the present application, and are not used to limit the present application.

[0028] As shown in Figures 1 to 12 , a sample pretreatment device of a flow cytometer of the present embodiment comprises a base 1, the top end of the base 1 is fixedly connected with a processing box 2, the top end of the processing box 2 is slidingly connected with a top sealing assembly 3, and the inner side of the processing box 2 is rotatably connected with a pretreatment assembly 4.

[0029] As Figures 2 to 8 shown, the base 1 bottom end four corners are respectively fixedly connected with shock pads 101, the base 1 top end middle part is fixedly connected with a first sliding groove 103, the first sliding groove 103 top end is rotatably connected with the mounting column 402, the base 1 top end is provided with a support column 102, the support column 102 is located at the outer ring position of the first sliding groove 103, the inner side of the support column 102 is in meshing sliding connection with the limiting boss 418, the two sides of the base 1 top end are respectively rotatably connected with a plurality of first pull rods 104, the base 1 middle part is fixedly connected with a motor 108, the motor 108 power output end is fixedly connected with the mounting column 402, so as to provide rotary power through the motor 108, drive the mounting column 402 and the whole pretreatment assembly 4 to rotate around the base 1, so as to realize uniform pretreatment of the sample in the test tube. The setting of a plurality of first pull rods 104 not only enhances the structural strength of the base 1, but also provides necessary support and guidance for the rotation of the pretreatment assembly 4, ensuring the stability and accuracy of the rotation process. At the same time, the bottom end of the first pull rod 104 is rotatably connected with the second pull rod 105, which increases the flexibility and stability of the device, so that the pretreatment assembly 4 can maintain a stable motion trajectory during rotation, avoiding shaking or deviation caused by rotation.

[0030] As Figures 2 to 8 shown, a plurality of first pull rods 104 bottom end are rotatably connected with second pull rods 105, two second pull rods 105 located on the same side are rotatably connected with a turntable 106, the turntable 106 middle part is rotatably connected with the inner wall of the base 1, the surface of the side of the turntable 106 away from the inner wall of the base 1 is rotatably connected with an electric push rod 107, the telescopic end of the electric push rod 107 is rotatably connected with the turntable 106, the end of the electric push rod 107 away from the turntable 106 is rotatably connected with the base 1, through the telescopic movement of the electric push rod 107, the turntable 106 is pushed to rotate, and then through the rotation connection between the turntable 106 and the base 1, the flexible adjustment of the distance between the base 1 is realized. Make them move synchronously, this design enhances the stability and flexibility of the device during pretreatment, when the telescopic end of the electric push rod 107 is elongated, the turntable 106 will rotate, and then drive the second pull rod 105 and the first pull rod 104 to move, the top sealing assembly 3 is synchronized and merged, which ensures that the center of gravity of the device is in the middle, so that the pretreatment assembly 4 can maintain a more stable posture during rotation.

[0031] As Figures 4 to 8As shown, the inside of the processing box 2 is provided with a hollow area 201, the inside of which is slidingly connected with the fan-shaped frame 405, the top end of the processing box 2 is fixedly connected with a limiting slide block 202 at four corners respectively, the limiting slide block 202 is slidingly connected with the top sealing assembly 3, the top end of the processing box 2 is fixedly connected with a sealing strip 203 on both sides, the both sides of the processing box 2 are provided with a first missing slot 204, the first missing slot 204 is slidingly fitted with the first pull rod 104, and the processing box 2 provides a closed and stable operating environment for the pretreatment assembly 4. The sliding connection design of the hollow area 201 and the fan-shaped frame 405 not only realizes the limiting and guiding of the pretreatment assembly 4 in the vertical direction, but also ensures the stability and accuracy of the pretreatment assembly 4 in the rotating process. The sliding connection of the limiting slide block 202 and the top sealing assembly 3 makes the top sealing assembly 3 can be opened or closed flexibly, which is convenient for the sample to be put in and taken out. At the same time, this design also enhances the sealing performance of the processing box 2, avoids the pollution or leakage of the sample in the pretreatment process. The setting of the sealing strip 203 further improves the sealing effect of the processing box 2, ensures the cleanliness and safety of the pretreatment environment. The sliding fitting design of the first missing slot 204 and the first pull rod 104 not only provides the necessary movement track for the first pull rod 104, but also makes the whole device more compact and reasonable in structure, avoids the problems of complex operation or space waste caused by too many components.

[0032] As Figures 5 to 8As shown, the capping assembly 3 includes two empty boxes 301, which are right trapezoidal structures, and the short right angle end of the empty box 301 is slidingly connected with a sliding door 302, the inner side of the empty box 301 is fixedly connected with a test tube rack 303, one side of the empty box 301 is installed with a ventilation fan 304, both sides of the long right angle end of the empty box 301 are provided with a second missing slot 305, the inner side of the second missing slot 305 is rotatably connected with a third pull rod 306, the third pull rod 306 is rotatably connected with the first pull rod 104, one side of the second missing slot 305 is provided with a limiting guide rail 307, the inner side of the limiting guide rail 307 is slidingly connected with the limiting sliding block 202, through the ingenious design of the capping assembly 3, the effective protection and convenient operation of the pretreatment assembly 4 in the treatment box 2 are realized. The two right trapezoidal structure empty boxes 301 not only provide sufficient space for the test tubes, but also through the design of the sliding door 302, the user can easily open or close the empty box 301, which is convenient for the test tube to be put in and taken out, and greatly improves the convenience of operation. The setting of the test tube rack 303 ensures the stability and safety of the test tube during the pretreatment process, avoids the loss or pollution of the sample caused by the shaking of the test tube. The installation of the ventilation fan 304 effectively promotes the air circulation in the empty box 301, which helps to maintain the constant temperature and humidity of the pretreatment environment, thereby improving the pretreatment effect. In addition, the rotatable connection of the third pull rod 306 and the first pull rod 104, and the meshing sliding connection of the limiting guide rail 307 and the limiting sliding block 202 not only enhance the connection stability between the capping assembly 3 and the treatment box 2, but also make the capping assembly 3 keep a smooth motion trajectory during the opening or closing process, avoid the shaking or deviation caused by the operation, and further improve the practicality and reliability of the device.

[0033] As Figures 9 to 11As shown, the pretreatment assembly 4 comprises a connecting plate 401, the bottom end of the connecting plate 401 is fixedly connected with a mounting column 402, the mounting column 402 is rotationally connected with the base 1, both ends of the connecting plate 401 are fixedly connected with a fan-shaped frame 405, the inner sides of the two fan-shaped frames 405 are slidingly connected with a double-layer sliding frame 406, the middle part of the mounting column 402 is fixedly connected with a connecting frame 403, both sides of the connecting frame 403 are rotationally connected with a test tube fixing frame 404, both ends of the test tube fixing frame 404 are fixedly connected with a protruding column 408, a plurality of curved grooves 407 are arranged in the middle part of the fan-shaped frame 405, the curved grooves 407 are slidingly connected with the protruding column 408 in a penetrating manner, the penetrating end of the protruding column 408 is located between one of the two layers of the double-layer sliding frame 406, a top column 410 is slidingly connected with the double-layer sliding frame 406 in a penetrating manner, the top end of the top column 410 is in sliding contact with the protruding column 408, a second sliding groove 414 is fixedly connected with the middle part of the top column 410, a convex circular plate 411 is slidingly connected with the inner side of the second sliding groove 414, the second sliding groove 414 is rotationally connected with the double-layer sliding frame 406, a single-head reel 415 is fixedly connected with the middle part of the convex circular plate 411, an electric motor 412 is fixedly connected with the power output end of the electric motor 412, a double-head reel 413 is frictionally connected with the single-head reel 415 between the double-head reel 413 and the single-head reel 415 through a transmission belt 416, the electric motor 412 provides power to drive the double-head reel 413 to rotate, the double-head reel 413 is frictionally connected with the single-head reel 415 through the transmission belt 416, so as to drive the convex circular plate 411 and the second sliding groove 414 to slide. This design not only realizes stable adjustment of the test tube fixing frame 404 in the vertical direction, but also ensures uniform stress of the test tube during the pretreatment process, avoids problems such as uneven cell crushing or sample loss caused by uneven stress, etc. At the same time, the sliding contact design of the top column 410 and the protruding column 408 further enhances the stability and reliability of the test tube fixing frame 404 during the adjustment process, and ensures the accuracy and reliability of the pretreatment result. In addition, the sliding connection design of the double-layer sliding frame 406 and the fan-shaped frame 405 not only realizes flexible adjustment of the test tube fixing frame 404, but also makes the whole pretreatment assembly 4 more compact and reasonable in structure, avoids problems such as complex operation or space waste caused by too many components, etc.

[0034] As shown in Figures 9 to 11 The middle part of the double-layer sliding frame 406 is fixedly connected with a hydraulic push rod 417, the bottom end of the hydraulic push rod 417 is fixedly connected with the fan-shaped frame 405, the telescopic end of the hydraulic push rod 417 is fixedly connected with the double-layer sliding frame 406, the bottom end of the fan-shaped frame 405 is fixedly connected with a limiting convex platform 418, the limiting convex platform 418 is slidingly connected with the base 1, a plurality of elastic push columns 409 are rotationally connected with the middle part of the test tube fixing frame 404.

[0035] As shown in Figures 9 to 12As shown, the placement opening of the test tube fixing frame 404 is fixedly connected with a U-shaped plate 419, the middle of the bottom end of the U-shaped plate 419 is slidably connected with a pressing table 420, the bottom end of the pressing table 420 is rotatably connected with a fourth pull plate 421, the end of the fourth pull plate 421 away from the pressing table 420 is rotatably connected with an L-shaped push plate 423, the middle of the L-shaped push plate 423 is rotatably connected with the U-shaped plate 419, and the end of the L-shaped push plate 423 away from the fourth pull plate 421 is fixedly connected with a clamping plate 422. Through the combination of the U-shaped plate 419, the pressing table 420, the fourth pull plate 421 and the L-shaped push plate 423, the test tube is clamped and fixed, and the shaking or falling of the test tube during the pretreatment process is avoided.

[0036] The working principle of the embodiment is as follows: The sample to be pretreated is placed in the test tube, and the test tube is placed on the test tube placing frame 303 in the empty box 301 of the capping assembly 3. By sliding the sliding plate door 302 at the short right angle end of the empty box 301, the test tube can be conveniently put in or taken out.

[0037] The capping assembly 3 is closed and slidably connected with the limiting sliding block 202 at the top end of the treatment box 2, so as to ensure the sealing of the treatment box 2. The sealing strip 203 further enhances the sealing effect of the treatment box 2, preventing the sample from being contaminated or leaking during the pretreatment process.

[0038] The motor 108 is started, and the power output end of the motor 108 drives the mounting column 402 to rotate. The rotation of the mounting column 402 drives the pretreatment assembly 4 as a whole through the connecting plate 401, so as to uniformly pretreat the sample in the test tube.

[0039] The test tube is placed in the placement opening of the test tube fixing frame 404. By adjusting the height of the pressing table 420, the fourth pull plate 421 will rotate, thereby driving the L-shaped push plate 423 to move, and finally making the clamping plate 422 clamp and fix the test tube. The extension end of the elastic push column 409 can adjust the pressure and angle of the test tube fixing frame 404 as needed, to ensure the stability of the test tube during the pretreatment process.

[0040] The electric motor 412 is started, and the power output end of the electric motor 412 drives the double-head reel 413 to rotate. The friction transmission connection between the double-head reel 413 and the single-head reel 415 drives the transmission belt 416 to rotate, thereby driving the boss circular plate 411 to slide in the second sliding groove 414. The sliding of the boss circular plate 411 drives the top column 410 to move, and the top column 410 is in sliding contact with the convex column 408, so that the test tube fixing frame 404 shakes on the double-layer sliding frame 406, the cells are broken, and the subsequent centrifugal treatment is facilitated.

[0041] The telescopic end of the hydraulic push rod 417 works to push the fan-shaped frame 405 to move in the direction of the curved groove 407, adjust the offset angle of the test tube fixing frame 404, and realize the centrifugal effect. The sliding connection of the limiting boss 418 and the base 1 ensures the stability of the fan-shaped frame 405 when adjusting the offset angle.

[0042] The design of the hydraulic push rod 417 provides vertical power support for the whole device. When it is necessary to adjust the height of the test tube, the telescopic end of the hydraulic push rod 417 can push the double-layer sliding frame 406 to slide in the fan-shaped frame 405, so as to adjust the height position of the test tube.

[0043] The telescopic end of the electric push rod 107 is rotationally connected with the rotating disc 106. Through the telescopic movement of the electric push rod 107, the rotating disc 106 can be driven to rotate, and then the movement track of the first pull rod 104 and the second pull rod 105 is adjusted, thereby assisting in adjusting the position and angle of the pretreatment assembly 4.

[0044] After the pretreatment is completed, the electric motor 412 and the motor 108 are turned off, the sliding plate door 302 of the capping assembly 3 is opened, the pretreated test tube is taken out, and the subsequent flow cytometer detection and analysis are prepared.

[0045] The above only describes the preferred embodiments of the present application and is not used to limit the protection scope of the present application.

Claims

1. A flow cytometer sample pretreatment device, comprising a base (1), characterized in that: The top of the base (1) is fixedly connected to a processing box (2), the top of the processing box (2) is slidably connected to a capping assembly (3), and the inside of the processing box (2) is rotatably connected to a pre-processing assembly (4); The pretreatment assembly (4) includes a connecting plate (401), the bottom end of the connecting plate (401) is fixedly connected to a mounting column (402), the mounting column (402) is rotatably connected to the base (1), both ends of the connecting plate (401) are fixedly connected to fan-shaped frames (405), the inner sides of the two fan-shaped frames (405) are slidably connected to double-layer slides (406), the middle of the mounting column (402) is fixedly connected to a connecting frame (403), both sides of the connecting frame (403) are rotatably connected to test tube fixing frames (404), and both ends of the test tube fixing frame (404) are fixedly connected to protruding columns (408); The fan-shaped frame (405) is provided with a plurality of curved grooves (407) in the middle thereof, the curved grooves (407) are slidably connected to the bosses (408), the through-end of the bosses (408) is located between one layer of the double-layer slide (406), the middle of the double-layer slide (406) is slidably connected to a top column (410), the top end of the top column (410) is in sliding contact with the bosses (408), the middle of the top column (410) is fixedly connected to a second slide groove (414), the second slide groove (414) A boss circular plate (411) is slidably connected on the inner side, the second slide groove (414) is rotatably connected to the double-layer slide (406), a single-head reel (415) is fixedly connected to the middle of the boss circular plate (411), an electric motor (412) is fixedly connected to the inner side of the double-layer slide (406), a double-head reel (413) is fixedly connected to the power output end of the electric motor (412), and a transmission belt (416) is frictionally connected between the double-head reel (413) and the single-head reel (415).

2. A flow cytometer sample pretreatment device according to claim 1, characterized in that: A hydraulic push rod (417) is fixedly connected to the middle of the double-layer slide (406), the bottom end of the hydraulic push rod (417) is fixedly connected to the fan-shaped frame (405), and the telescopic end of the hydraulic push rod (417) is fixedly connected to the double-layer slide (406).

3. A flow cytometer sample pretreatment device according to claim 1, characterized in that: The bottom end of the fan-shaped frame (405) is fixedly connected to a limiting boss (418), and the limiting boss (418) is slidably connected to the base (1). The middle part of the mounting column (402) is rotatably connected to a plurality of elastic push columns (409), and the telescopic end of the elastic push column (409) is rotatably connected to the middle part of the test tube fixing frame (404).

4. A flow cytometer sample pretreatment device according to claim 1, characterized in that: The inner side of the placement port of the test tube fixing rack (404) is fixedly connected to a U-shaped plate (419), and the middle part of the bottom end of the U-shaped plate (419) is slidably connected to a press platform (420), and the two sides of the bottom end of the press platform (420) are rotatably connected to the fourth pull plate (421), and the end of the fourth pull plate (421) away from the press platform (420) is rotatably connected to an L-shaped push plate (423), the middle part of the L-shaped push plate (423) is rotatably connected to the U-shaped plate (419), and the end of the L-shaped push plate (423) away from the fourth pull plate (421) is fixedly connected to a clamping plate (422).

5. A flow cytometer sample pretreatment device according to claim 1, characterized in that: The four corner positions at the bottom of the base (1) are respectively fixedly connected with shock-absorbing pads (101), the middle part of the top of the base (1) is fixedly connected with a first slide groove (103), the top of the first slide groove (103) is rotatably connected to the mounting column (402), the top of the base (1) is provided with a support column (102), the support column (102) is located at the outer ring position of the first slide groove (103), the inner side of the support column (102) is engaged and slidably connected with the limiting boss (418), the top of both sides of the base (1) are respectively rotatably connected with a plurality of first pull rods (104), the middle part of the base (1) is fixedly connected with a motor (108), and the power output end of the motor (108) is fixedly connected to the mounting column (402).

6. A flow cytometer sample pretreatment device according to claim 5, characterized in that: The bottom ends of several first pull rods (104) are rotatably connected to second pull rods (105), and a turntable (106) is rotatably connected between two second pull rods (105) located on the same side. The middle of the turntable (106) is rotatably connected to the inner wall of the base (1), and the surface of the turntable (106) away from the inner wall of the base (1) is rotatably connected to an electric push rod (107). The telescopic end of the electric push rod (107) is rotatably connected to the turntable (106), and the end of the electric push rod (107) away from the turntable (106) is rotatably connected to the base (1).

7. A flow cytometer sample pretreatment device according to claim 1, characterized in that: A hollow area (201) is provided on the inner side of the processing box (2), and the inner side of the hollow area (201) is slidably connected to the fan-shaped frame (405). The four corners of the top of the processing box (2) are fixedly connected to the limiting sliders (202), and the limiting sliders (202) are slidably connected to the capping assembly (3). Sealing strips (203) are fixedly connected to both sides of the top of the processing box (2). First notches (204) are provided on both sides of the processing box (2), and the first notches (204) are slidably fitted with the first pull rod (104).

8. A flow cytometer sample pretreatment device according to claim 1, characterized in that: The capping assembly (3) includes two empty boxes (301), the empty boxes (301) are of a right-angled trapezoidal structure, the short right-angled end of the empty box (301) is slidably connected to a slide door (302), the inner side of the empty box (301) is fixedly connected to a test tube placement rack (303), a ventilation fan (304) is installed on one side of the empty box (301), and second notches (305) are provided on both sides of the long right-angled end of the empty box (301), the inner side of the second notch (305) is rotatably connected to a third pull rod (306), the third pull rod (306) is rotatably connected to the first pull rod (104), a limiting guide rail (307) is provided on one side of the second notch (305), and the inner side of the limiting guide rail (307) is engaged and slidably connected with the limiting slider (202).

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