Western blot processing device

Abstract

The invention belongs to the technical field of protein antibody incubation, and provides a western blot processing device, which comprises: a base; the limiting column is fixedly arranged on the base, one end of the limiting column is provided with a cam component, and the cam component is provided with at least one convex part and / or one concave part; the rotary disc is rotationally arranged relative to the limiting column and comprises a first fixing disc and a second fixing disc, the cam component is located between the first fixing disc and the second fixing disc, and a preset distance is formed between the cam component and the second fixing disc; the at least one first push rod is movably arranged on the lower side face of the second fixing disc, one end of the first push rod can abut against the cam component, and the cam component can drive the first push rod to move in a reciprocating mode in the axis direction of the first push rod; the reaction tank is movably arranged on the upper side face of the second fixing disc and penetrates through the second fixing disc to be movably connected with the other end of the first push rod. With the adoption of the structure, the swing of the reaction tank can be realized by virtue of the cam component and the first push rod while the turntable rotates.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of protein antibody incubation, in particular to a protein blotting processing device. BACKGROUND

[0002] Western blot is an experimental technique used to detect and analyze specific proteins, widely used in biomedical research. Its main steps are as follows: the proteins in the sample are separated by polyacrylamide gel electrophoresis (PAGE), separated according to the molecular weight of the protein, the separated protein is transferred to a solid support (such as PVDF or nitrocellulose membrane), the protein is fixed on the membrane, then the non-specific binding sites on the membrane are blocked with proteins that do not contain the target protein (such as bovine serum albumin BSA or skim milk powder) to reduce background noise. First, incubate the membrane with specific primary antibodies, which bind to the target protein, then use secondary antibodies (usually labeled with enzymes or fluorescent dyes) to detect the primary antibody-bound target protein. By chemiluminescence, fluorescence or colorimetric method to detect the bound secondary antibody, so as to identify the target protein and analyze its expression level.

[0003] The existing protein blotting processing device is mostly semi-automatic processing structure, and the existing device is independent of each other, the driving device is more, the structure equipment is more complex, and the volume is relatively large, in addition, manual participation is required for part of the process, and full automation operation cannot be realized. SUMMARY

[0004] The present application provides a protein blotting processing device, which solves the problems of the existing device being semi-automatic processing structure, not being able to realize true full automation processing, and the existing device being independent of each module, having more driving devices, and having large volume.

[0005] In order to achieve the above purpose, the technical scheme adopted by the present application is as follows: A protein blotting processing device, comprising: a base; a limiting column, one end of which is fixedly arranged on the base, the limiting column being provided with a preset length, and the other end of the limiting column away from the base being provided with a fixedly arranged cam component, the cam component being provided with at least one recess and / or one protrusion; a turntable, which is rotatably arranged relative to the limiting column, the turntable comprising a first fixed disc and a second fixed disc, the first fixed disc being arranged on the side close to the base, and the second fixed disc being arranged on the side close to the cam component, the cam component being located between the first fixed disc and the second fixed disc, and the cam component and the second fixed disc being provided with a preset distance, At least one first push rod movably arranged on the lower side of the second fixed disc, one end of the first push rod being capable of abutting against the cam component, the first push rod being capable of reciprocating along the axis thereof; A reaction tank movably arranged on the upper side of the second fixed disc, the reaction tank being provided with a first connecting portion, the second fixed disc being provided with a first through slot, the first connecting portion being movably connected with the other end of the first push rod through the first through slot, the first push rod being capable of driving the reaction tank to reciprocate relative to the second fixed disc.

[0006] In some embodiments, the contact end of the first push rod and the cam component is provided with a first roller, the first roller being rotatably arranged on the first push rod, the first roller being capable of abutting against the cam component.

[0007] In some embodiments, the recess and the protrusion are both arc surfaces, and the protrusion and the recess are connected in a smooth transition.

[0008] In some embodiments, the lower side of the second fixed disc is provided with a first sliding groove, the first push rod being movably arranged in the first sliding groove, the two sides of the first sliding groove being provided with a plurality of second rollers, the plurality of second rollers all abutting against the side surface of the first push rod.

[0009] In some embodiments, a first return spring is further included, one end of the first return spring being connected with the outside of the first sliding groove, the other end of the first return spring being connected with the first push rod.

[0010] In some embodiments, an adaptive incubation box is arranged in the reaction tank, the incubation box being detachably arranged on the reaction tank.

[0011] In some embodiments, a third fixed disc is further included, the third fixed disc being arranged between the first fixed disc and the second fixed disc, the third fixed disc being connected with the first fixed disc and the second fixed disc respectively; A plurality of test tube assemblies are arranged on the second fixed disc and the third fixed disc, the test tube assembly including a first support arranged on the second fixed disc and a heat preservation cavity arranged on the third fixed disc, the first support being provided with a plurality of first mounting holes, the first mounting holes being in communication with the heat preservation cavity, the heat preservation cavity being provided with a first adapter and a first refrigeration sheet, the first adapter being used for supporting the bottom of a test tube, and the first refrigeration sheet being used for refrigeration in the heat preservation cavity.

[0012] In some embodiments, a first radiator and a temperature monitoring sensor are further provided on the outside of the heat preservation cavity. The first radiator includes a plurality of first heat dissipating fins spaced apart from each other. The temperature monitoring sensor is used to detect the temperature inside the heat preservation cavity.

[0013] In some embodiments, a cleaning groove is further provided on the first bracket, and the cleaning groove includes a first cleaning hole and a second cleaning hole. The inner diameter of the first cleaning hole is larger than the inner diameter of the second cleaning hole, and the depth of the first cleaning hole is larger than the depth of the second cleaning hole.

[0014] In some embodiments, central axes of the plurality of first mounting holes, the first cleaning holes, and the second cleaning holes are all located on the same circumferential line.

[0015] In some embodiments, the first supports and the reaction tanks are alternately arranged on the second fixed plate.

[0016] In some embodiments, the refrigerator further comprises a second refrigeration fin and a refrigeration base, wherein the second refrigeration fin is fixedly arranged relative to the third fixed plate, and the second refrigeration fin is connected to the refrigeration base; Wherein, the reaction tank is provided with a second notch, a refrigeration contact seat is provided at the second notch, the refrigeration contact seat is in contact with the incubation box, and one end of the refrigeration seat abuts against the refrigeration contact seat.

[0017] In some embodiments, a smooth guide structure is provided between the refrigeration contact seat and the refrigeration seat, and the smooth guide structure includes a first arcuate groove provided on the refrigeration contact seat and a first arcuate boss provided at one end of the refrigeration seat, and the first arcuate groove is adapted to the first arcuate boss, wherein the rotation axis of the refrigeration contact seat relative to the second fixed disk coincides with the center of the first arcuate groove.

[0018] In some embodiments, a first thermal insulation seat is further provided on the third fixed disk, and a first refrigeration channel is provided on the first thermal insulation seat. The refrigeration seat abuts against the refrigeration contact seat through the first refrigeration channel, wherein a first clearance groove is provided on the first thermal insulation seat, and the position of the first clearance groove corresponds to the position of the first connecting part, and the first push rod can be extended into the first clearance groove and connected to the first connecting part.

[0019] In some embodiments, a second heat sink is provided on the first thermal insulation seat, and the second heat sink includes a plurality of second heat dissipation fins arranged in parallel, and the second heat dissipation fins are located on the lower side of the third fixed plate.

[0020] In some embodiments, the device further comprises a liftable liquid suction needle, wherein the liquid suction needle is provided with a first liquid suction channel, and one end of the liquid suction needle is provided with a first fixing block, and the first fixing block is provided with a first sub-channel, a second sub-channel and a third sub-channel, and the first sub-channel, the second sub-channel and the third sub-channel are in communication with the first liquid suction channel.

[0021] In some embodiments, the device further comprises a waste liquid pump, a pure water pump and a sealing liquid pump arranged on the base, wherein the waste liquid pump is in communication with the first sub-channel through a first valve, the pure water pump is in communication with the second sub-channel through a second valve, and the sealing liquid pump is in communication with the third sub-channel through a third valve.

[0022] In some embodiments, a plunger pump is arranged between the second valve and the second sub-channel, and the plunger pump is in series with the second valve.

[0023] In some embodiments, the device further comprises a first connecting pipe, a first lifting block, a first lifting fixing block and a second lifting block, wherein the second lifting block is connected with the first lifting fixing block, and a preset interval is arranged between the second lifting block and the first lifting block, one end of the first connecting pipe is connected with the first fixing block, the other end of the first connecting pipe is connected with the first lifting block, the first lifting block is arranged on the first lifting fixing block, and the first lifting block can be separated from the first lifting fixing block, the moving direction of the first lifting block, the first lifting fixing block and the second lifting block is consistent, and the central axis of the first connecting pipe is parallel with the central axis of the liquid suction needle.

[0024] In some embodiments, the device further comprises a first connecting rod and a first guide rod arranged in parallel with each other, wherein the first guide rod is provided with a first guide sleeve, the first guide sleeve is connected with the first lifting block, the first lifting block is movably arranged on the first guide rod, and the first connecting rod passes through the first lifting block and is connected with the first lifting fixing block.

[0025] Compared with the prior art, the device has the following beneficial effects: The application sets a limiting column on the base, one end of the limiting column is fixedly arranged relative to the base, a cam component is arranged on the other end of the limiting column, at least one recess and / or protrusion is arranged on the cam component, a rotating disc is rotatably arranged on the limiting column, at least one first push rod is arranged on a second fixed disc of the rotating disc, the first push rod can reciprocatingly move in the axial length direction of the first push rod relative to the second fixed disc, one end of the first push rod abuts against the cam component, the other end of the first push rod is movably connected with a first connecting part of a reaction tank swingably arranged on the second fixed disc, the rotating disc drives the first push rod to synchronously rotate, so that the abutting position of the first push rod on the cam component changes, thereby the first push rod reciprocatingly moves, since the first push rod is connected with the reaction tank, the reaction tank can swing relative to the second fixed disc during the rotation of the rotating disc, only one driving device can drive the reaction tank to change the position relative to the limiting column, and the reaction tank can swing at the same time, so that the substances in the reaction tank are well mixed.

[0026] Additional aspects and advantages of the application will be set forth in part in the description which follows, and in part will become apparent to those skilled in the art upon examination of the following and / or can be learned by practice of the application. BRIEF DESCRIPTION OF DRAWINGS

[0027] Figure 1 It is a first perspective view of a protein blot processing device of the application; Figure 2 It is an exploded view of a rotating disc of a protein blot processing device of the application; Figure 3 It is Figure 2 Another angle view; Figure 4 It is a top view of a reaction tank transmission structure of a protein blot processing device of the application; Figure 5 It is a perspective view of a reaction tank transmission structure of a protein blot processing device of the application; Figure 6 It is a perspective view of a test tube assembly of a protein blot processing device of the application; Figure 7 It is Figure 6 An exploded view in Figure 8 It is Figure 7 A sectional view of a cleaning tank in Figure 9 It is an exploded view of a refrigeration module of a reaction tank of a protein blot processing device of the application; Figure 10 It is a sectional view of a refrigeration module of a reaction tank of a protein blot processing device of the application; Figure 11Simplified schematic diagram of the reaction tank transmission structure of a protein blotting treatment device according to the present application; Figure 12 Exploded diagram of the internal structure of a reaction tank of a protein blotting treatment device according to the present application; Figure 13 Schematic diagram of the driving structure of a rotating disc of a protein blotting treatment device according to the present application; Figure 14 Schematic diagram of the driving structure of a rotating disc of a protein blotting treatment device according to the present application; Figure 13 Schematic diagram of the driving structure of a rotating disc of a protein blotting treatment device according to the present application; Figure 15 Schematic diagram of the driving structure of a rotating disc of a protein blotting treatment device according to the present application; Figure 16 Second perspective view of a protein blotting treatment device according to the present application; Figure 17 Second perspective view of a protein blotting treatment device according to the present application; Figure 16 Second perspective view of a protein blotting treatment device according to the present application; Second perspective view of a protein blotting treatment device according to the present application; Figure 18 Exploded diagram of the lifting structure of a sampling needle of a protein blotting treatment device according to the present application; Exploded diagram of the lifting structure of a sampling needle of a protein blotting treatment device according to the present application; Figure 19 Exploded diagram of the lifting structure of a sampling needle of a protein blotting treatment device according to the present application; Exploded diagram of the lifting structure of a sampling needle of a protein blotting treatment device according to the present application; Figure 20 Schematic diagram of the structure of a pump body portion of a protein blotting treatment device according to the present application; Schematic diagram of the structure of a pump body portion of a protein blotting treatment device according to the present application; Figure 21 Schematic diagram of the structure of a pump body portion of a protein blotting treatment device according to the present application; DETAILED DESCRIPTION

[0028] The present application will be further described in conjunction with the specific drawings. In the description of the present embodiment, unless otherwise specified, the terms "left", "right", and the like indicate the orientation or positional relationship shown in the drawings, and are merely used to facilitate the description of the present application and simplify the description, and therefore cannot be understood as limiting the present application to a specific orientation, configuration, and operation.

[0029] As Figure 1 , Figure 2 , Figure 5 and Figure 13As shown, the protein blotting device provided by the present application comprises a base 1000, a limiting column 200 is arranged on the base 1000, one end of the limiting column 200 is fixedly arranged relative to the base 1000, the limiting column 200 is provided with a preset length, wherein a fixed cam component 201 is arranged at the end of the limiting column 200 away from the base, the cam component 201 has a certain thickness, at least one protrusion 20111 and / or one recess 20112 are arranged on the cam component 201; for example, only one protrusion 20111 or only one recess 20112 is arranged on the cam component 201, or the protrusion 20111 and the recess 20112 are alternately arranged.

[0030] A rotating disc is rotatably arranged on the limiting column 200, the rotating disc comprises a first fixed disc 101 and a second fixed disc 102, the first fixed disc 101 is arranged close to one side of the base 1000, the second fixed disc 102 is arranged close to the cam component 201, and the cam component 201 is located between the first fixed disc 101 and the second fixed disc 102, a preset interval is arranged between the second fixed disc 102 and the cam component 201, so as to ensure that the second fixed disc 102 can rotate relative to the cam component 201. At least one first push rod 1022 is movably arranged on the lower side of the second fixed disc 102, for example, Figure 4 and Figure 5 As shown, one end of the first push rod 1022 can abut against the cam component 201, and during the rotation of the rotating disc, one end of the first push rod 1022 always abuts against the cam component 201. For example, one end of the first push rod 1022 abuts against the recess 20112 or abuts against the protrusion 20111, and due to the rotation of the rotating disc, the position of the first push rod 1022 relative to the limiting column 200 changes, under the action of the cam component 201, the first push rod 1022 can move along the length direction of the axis thereof in a reciprocating straight line manner; A reaction tank 1028 is movably arranged on the upper side of the second fixed disc 102, the number of the reaction tanks 1028 is consistent with the number of the first push rods 1022, a first connecting part 10281 is arranged on the reaction tank 1028, for example, Figure 12 As shown, a first slot 1021 is arranged on the second fixed disc 102, the first connecting part 10281 passes through the first slot 1021 and movably connects with the other end of the first push rod 1022, through the reciprocating straight line movement of the first push rod 1022, the reaction tank 1028 can be driven to reciprocating swing relative to the second fixed disc 102, so that the antibody in the reaction tank 1028 and the blocking film are fully shaken and combined, and antibody incubation is performed. In the embodiment, the reaction tank 1028 is rotatably arranged on the second fixed disc 102 through hole shaft cooperation.

[0031] The application sets the limiting column 200 on the base 1000, sets the cam component 201 on the limiting column 200, sets the first push rod 1022 on the second fixed disc 102, rotates the rotating disc relative to the limiting column 200, so that one end of the first push rod 1022 can be switched in the concave part 20112 and the convex part 20111 of the cam component 201, drives the first push rod 1022 to move linearly, so that the reaction tank 1028 movably connected with the other end of the first push rod 1022 can swing relative to the second fixed disc 102, the antibody and the blocking film in the reaction tank 1028 are fully mixed and incubated, and one driving part drives the rotating disc to rotate and the reaction tank 1028 to swing.

[0032] As a preferred embodiment, in the embodiment, the movable connection mode of the first push rod 1022 and the first connecting part 10281 of the reaction tank 1028 is as shown in Figure 11 and 12 , a through hole is arranged at one end of the first push rod 1022, a corresponding through U-shaped groove 102810 is arranged on the first connecting part 10281, and the two are connected through a pin shaft. By arranging the U-shaped groove 102810, the movement interference of the reaction tank 1028 in the vertical direction of the plane of the second fixed disc 102 when swinging can be avoided.

[0033] Alternatively, the U-shaped groove 102810 can also be replaced by a larger diameter through hole, so as to compensate for the movement gap of the first connecting part 10281 in the direction perpendicular to the plane of the second fixed disc 102.

[0034] Alternatively, the U-shaped groove 102810 can also be arranged on the first push rod 1022, wherein the length direction of the U-shaped groove 102810 should be best in the direction perpendicular to the second fixed disc 102, and the U-shaped groove 102810 can also be arranged in the direction perpendicular to the plane of the second fixed disc 102, as long as the reaction tank 1028 can swing relative to the second fixed disc 102810.

[0035] As shown in Figure 11 , the cam component 201 includes four concave parts 20112 and four convex parts 20111, and four first push rods 1022 and four reaction tanks 1028 are correspondingly arranged. According to experimental requirements, the number of reaction tanks 1028 and first push rods 1022 can be set. For example, the number of concave parts 20112 and convex parts 20111 can be three or five, or other numbers, and the number is not limited by the application, as long as it can be installed on the second fixed disc 102.

[0036] In one embodiment, as shown in Figure 4 and Figure 5As shown, as a preferred embodiment, a first roller 1023 is arranged at the contact end of the first push rod 1022 and the cam component 201, the first roller 1023 is rotatable relative to the first push rod 1022, and the first roller 1023 is in abutment with the cam component 201. During the rotation of the rotating disc, the first roller 1023 is arranged in a rolling structure, which reduces the frictional resistance between the first push rod 1022 and the cam component 201, so that the rotation of the rotating disc is smoother. In the embodiment, the first roller 1023 is provided with a preset width, and the cam component 201 is provided with a matching thickness, so as to ensure the contact surface of the first roller 1023 and the cam component 201, and ensure the stability of the transmission between the first push rod 1022 and the cam component 201.

[0037] Further, the concave portion 20112 and the convex portion 20111 of the cam component 201 are both arc surface structures, and the convex portion 20111 and the concave portion 20112 are connected in a smooth transition. Specifically, as shown in Figure 4 、 Figure 5 and Figure 13 , the smooth transition means that the connection between the convex portion 20111 and the concave portion 20112 of the cam component 201 is tangent or connected by a smooth plane, and the convex portion 20111 and the concave portion 20112 are both tangent to the smooth plane. By using the above structure, the contact between the first roller 1023 and the cam component 201 is a tangent structure, which can reduce the contact surface between the first roller 1023 and the cam component 201 on the first push rod 1022, and further reduce the frictional resistance between the first roller 1023 and the cam component 201 during the rotation of the rotating disc, in combination with the first roller 1023 arranged to rotate.

[0038] In an embodiment, as shown in Figure 3 、 Figure 4 and Figure 5 , in order to facilitate the reciprocating linear movement of the first push rod 1022 relative to the axial length direction of the first push rod 1022, a first sliding groove is arranged on the lower side of the second fixed disc 102, and the first push rod 1022 is movably arranged in the first sliding groove. Specifically, as shown in Figure 19 , the first sliding groove is composed of a sliding groove base 1026 and a sliding groove cover plate 1025, the sliding groove base 1026 is arranged on the lower side of the second fixed disc 102, and the sliding groove cover plate 1025 is detachably arranged on the sliding groove base 1026. A first slot is arranged on the sliding groove base 1026, and the sliding groove cover plate 1025 covers the first slot to form the first sliding groove. The sliding groove cover plate 1025 supports the first push rod 1022 in the vertical direction of the plane where the second fixed disc 102 is located.

[0039] Optionally, the first sliding groove structure can also be a shaft sleeve structure, for example, the first push rod 1022 is a cylindrical structure, the shaft sleeve is sleeved on the lower side of the second fixed disc 102, and the first push rod 1022 can move axially relative to the shaft sleeve, which can also achieve the above-mentioned purpose.

[0040] Further, a plurality of second rollers 1024 are arranged on both sides of the first sliding groove, and the plurality of second rollers 1024 are in abutment with the side surface of the first push rod 1022. Specifically, as shown in Figure 4 and Figure 5 , the second roller 1024 includes four, which are respectively arranged in two groups at both ends of the sliding groove base 1026, and the four second rollers 1024 are rotatably arranged relative to the second fixed disc 102. The four second rollers 1024 pass through the sliding groove base 1026 and are fixedly connected with the second fixed disc 102. When the second roller 1024 is installed, the sliding groove base 1026 can also be fixed at the same time, which simplifies the assembly process of the sliding groove base 1026. In the embodiment, when the second roller 1024 is installed, the side surface of the first push rod 1022 in contact with the second roller 1024 does not contact the first sliding groove, and the first sliding groove only supports the first push rod 1022 at this time, so that the first push rod 1022 reduces the contact surface between the first push rod 1022 and the first sliding groove during movement, and at the same time, part of the sliding friction is converted into rolling friction, which reduces the frictional resistance of the first push rod 1022 during movement, and makes the reciprocating linear movement of the first push rod 1022 more smooth.

[0041] In one embodiment, as shown in Figure 4 , Figure 5 and Figure 19 , in order to facilitate the movement of the first push rod 1022 from the convex part 20111 of the cam member 201 to the concave part 20112 of the cam member 201, a first return spring (not shown in the figure) is further included, and the first push rod 1022 is moved from the convex part 20111 to the concave part 20112 through the first return spring. Specifically, a first connecting column 10252 is arranged on the first push rod 1022, a second connecting column 10251 is arranged on the sliding groove cover plate 1025, one end of the first return spring is connected with the first connecting column 10252, the other end of the first return spring is connected with the second connecting column 10251, and the first return spring is arranged outside the first sliding groove. Through the above structure, the extension direction of the first return spring is parallel to the moving direction of the first push rod 1022, so that the elastic force generated by the first return spring is completely used as the power for the movement and reset of the first push rod 1022.

[0042] Optionally, the first connecting column 10252 can be arranged at one end of the first push rod 1022 close to the reaction tank 1028. When the first push rod 1022 moves from the convex portion 20111 to the concave portion 20112 of the cam member 201, the first reset spring is in a stretched state. When the rotary disc continues to rotate, the first push rod 1022 moves from the convex portion 20111 to the concave portion 20112 of the cam member 201 under the action of the first reset spring.

[0043] Optionally, the first connecting column 10252 can also be arranged between the first sliding groove and the cam member 201. When the first push rod 1022 moves to one side of the reaction tank 1028, the first reset spring is compressed. When the rotary disc continues to rotate, the first push rod 1022 can move from the convex portion 20111 to the concave portion 20112 of the cam member 201 under the action of the first reset spring.

[0044] In one embodiment, as shown in Figure 12 In order to facilitate the binding of antibodies to the sealing film and the incubation of antibodies, a detachable incubation box 10284 is arranged in the reaction tank 1028. The shape of the incubation box 10284 is adapted to the internal structure of the reaction tank 1028, so as to ensure that the position of the incubation box 10284 in the reaction tank 1028 is relatively fixed. In this embodiment, the incubation box 10284 can only be used for single antibody incubation. Optionally, a partition can be arranged in the incubation box 10284 to divide the incubation box 10284 into multiple independent parts, such as 2, 3 or 4 independent parts, so as to meet different antibody incubation requirements. Optionally, the incubation box 10284 can also be disposable.

[0045] In one embodiment, as shown in Figure 2 and Figure 3 The rotary disc further comprises a third fixed disc 103. The third fixed disc 103 is arranged to rotate relative to the limiting column 200. The third fixed disc 103 is arranged between the first fixed disc 101 and the second fixed disc 102. The third fixed disc 103 is fixedly connected to the first fixed disc 101 through a plurality of first fixed columns 1012. The third fixed disc 103 is arranged in parallel to the first fixed disc 101 and the second fixed disc 102. The third fixed disc 103 is fixedly connected to the second fixed disc 102 through a plurality of second fixed columns 1033. A plurality of test tube assemblies are arranged on the second fixed disc 102 and the third fixed disc 103, as shown in Figure 1 , Figure 6 and Figure 7As shown, the test tube assembly includes a plurality of test tubes of different sizes, which are used to place different antibodies or washing liquids. Specifically, the test tube assembly includes a first support 10271 arranged on the second fixed disc 102 and a heat preservation shell 10311 arranged on the third fixed disc 103, and the heat preservation shell 10311 is provided with a heat preservation cavity 10316. A plurality of first mounting holes are arranged on the first support 10271, and a plurality of first through holes 1027 corresponding in position, size and number to the first mounting holes are arranged on the second fixed disc 102. As shown in Figure 4 As shown, the plurality of first mounting holes are in communication with the heat preservation cavity 10316, and a first adapter 10317 is arranged in the heat preservation cavity 10316. The first adapter 10317 is provided with a plurality of first support positions adapted to the test tubes, and is mainly used for mounting and placing the plurality of test tubes and supporting the bottom of the plurality of test tubes. In addition, a first cooling fin 10318 is arranged in the heat preservation cavity 10316, which is used for cooling in the heat preservation cavity 10316 to achieve the required temperature of the antibodies and reagents in the test tubes. In order to facilitate assembly, an opening in communication with the heat preservation cavity 10316 is arranged on the side of the heat preservation shell 10311, and a heat preservation foam block 10319 is arranged at the opening to seal the opening. The first cooling fin is arranged on the heat preservation foam block 10319 and located in the heat preservation cavity 10316, thereby facilitating the assembly of the first cooling fin 10318.

[0046] Further, the outer side of the heat preservation shell 10311 is also provided with a first heat sink 10314 and a temperature detection sensor 10312. The first heat sink 10314 is arranged on the lower side of the heat preservation shell 10311, as shown in Figure 3 and Figure 6 As shown, a plurality of first mounting slots 1032 are arranged on the third fixed disc 103, and the heat preservation shell 10311 is fixed at the first mounting slots 1032. The first heat sink 10314 includes a plurality of first heat dissipation fins arranged at intervals, which are connected through a plurality of first heat dissipation pipes 10315. One end of the first heat dissipation pipe 10315 is provided with a first heat dissipation block 10313, and the first heat sink 10314 is fixed on the outer wall of the heat preservation cavity 10316 through the first heat dissipation block 10313. The temperature detection sensor 10312 is arranged in the heat preservation cavity 10316, which is used for real-time monitoring of the real-time temperature in the heat preservation cavity 10316, so as to realize real-time regulation of the temperature in the heat preservation cavity through the first cooling fin 10318.

[0047] In one embodiment, in order to simplify the operation steps of the device, the first support 10271 and the reaction tank 1028 are alternately arranged, and the first support 10271 and the reaction tank 1028 are arranged close to the outer edge of the second fixed disc 102. In this embodiment, the first support 10271 and the reaction tank 1028 are both four, and the two are alternately arranged, wherein each first support 10271 can correspond to a reaction tank 1028, that is, a plurality of test tubes on the first support 10271 contain consumables required for the same experiment. For example, in this embodiment, two large-capacity test tubes and two small-capacity test tubes are used. Generally, since the demand for antibody reagents is small, two small-capacity test tubes are generally used to place antibody reagents, and two large-capacity test tubes are generally used to place corresponding washing liquids. For example, when A reagent antibody is used, the corresponding washing liquid for the A reagent antibody is placed. When B antibody reagent is used, the corresponding washing liquid for the B antibody reagent is used. When the demand for reagents is large, two large-capacity test tubes can also be used to place antibody reagents, and two small-capacity test tubes can also be used to place corresponding washing liquids. The specific application is set according to the actual needs of the user.

[0048] Further, in this embodiment, in order to facilitate distinction, the same color appearance is also used to mark on the same group of first supports 10271 and reaction tanks 1028. In this embodiment, the first supports 10271 and the reaction tanks 1028 are divided into four groups, and four different color appearances are used, for example, red, white, black, and blue, so that the user can know the corresponding experimental situation in each group of first supports 10271 and reaction tanks 1028 at any time.

[0049] In one embodiment, as shown in Figure 9 and Figure 10 In order to provide a more stable incubation temperature for the reaction tank 1028, a second refrigeration sheet 10324 and a refrigeration seat 10323 are also included. The second refrigeration sheet 10324 is fixedly arranged relative to the third fixed disc 103, and the second refrigeration sheet 10324 and the refrigeration seat 10323 are connected. As shown in Figure 12 , a second slot 10282 is arranged on the reaction tank 1028, and a refrigeration contact seat 10283 is arranged at the second slot 10282. The refrigeration contact seat 10283 is provided with a refrigeration transfer surface of a certain area in the reaction tank 1028. The refrigeration transfer surface is in full contact with the incubation box 10284 to ensure the refrigeration transfer efficiency. The refrigeration seat 10323 is in abutment with the refrigeration contact seat 10283 at one end, so as to realize heat transfer.

[0050] Further, since the reaction tank 1028 is arranged to rotate relative to the second fixed disc 102, in order to ensure the transmission effect of refrigeration, and to ensure that the reaction tank 1028 does not interfere with movement during rotation, a smooth guiding structure is arranged between the refrigeration contact seat 10283 and the refrigeration seat 10323. Specifically, the smooth guiding structure comprises a first arc-shaped groove 102831 arranged on the refrigeration contact seat 10283 and a first arc-shaped boss 103231 arranged at one end of the refrigeration seat 10323, the first arc-shaped boss 103231 and the first arc-shaped groove 102831 are matched, thereby increasing the contact area of the refrigeration seat 10323 and the refrigeration contact seat 10283, and ensuring the transmission efficiency of the refrigeration effect. It should be noted that the rotation axis of the reaction tank 1028 relative to the second fixed disc 102 coincides with the center position of the first arc-shaped groove 102831, thereby ensuring that the refrigeration seat 10323 does not lift the refrigeration contact seat 10283 during the rotation of the reaction tank 1028, the relative position of the contact between the refrigeration contact seat 10283 and the refrigeration seat 10323 does not change, and the reagent mixed solution in the incubation box 10284 on the reaction tank 1028 is prevented from spilling out.

[0051] Alternatively, the first arc-shaped groove 102831 can also be arranged on the refrigeration seat 10323, and the first arc-shaped boss 103231 can be arranged on the refrigeration contact seat 10283, which can also achieve the above-mentioned effect.

[0052] In one embodiment, in order to achieve heat insulation and avoid the influence of the heat inside the device on the refrigeration effect during the process of the second refrigeration sheet 10324, a first heat insulation seat 10321 is further arranged. Specifically, as shown in FIG. 10, the first heat insulation seat 10321 is arranged between the second fixed disc 102 and the refrigeration seat 10323, and the first arc-shaped boss 103231 is arranged on the first heat insulation seat 10321. Figure 3 and Figure 9As shown, a second mounting notch 1031 is provided on the third fixed disk 103, and a first heat-insulating seat 10321 is fixedly provided at the second mounting notch 1031 of the third fixed disk 103. A through first refrigeration channel 10322 is provided on the first heat-insulating seat 10321. The refrigeration seat 10323 passes through the first refrigeration channel 10322 and abuts against the refrigeration contact seat 10283 for refrigeration transfer. It should be noted that, since the first push rod 1022 is connected to the first connecting portion 10281 of the reaction tank 1028, the refrigeration seat 10323 is just below the reaction tank 1028. Therefore, A first give way groove 103210 is provided on the first thermal insulation seat 10321. The position of the first give way groove 103210 corresponds to the position of the first connecting part 10281. The first connecting part 10281 can be located in the first give way groove 103210, and when the first connecting part 10281 rotates following the reaction tank 1028, the first connecting part 10281 does not contact the first give way groove 103210. Part of the first push rod 1022 is connected to the first connecting part 10281 through the first give way groove 103210, which can achieve thermal insulation while avoiding movement interference with the reaction tank 1028.

[0053] Furthermore, in order to ensure heat dissipation, a second heat sink 10325 is provided below the first heat insulation seat 10321. The second heat sink 10325 is similar in structure to the first heat sink 10314 and includes a plurality of second heat sink fins arranged in parallel and spaced apart. The second heat sink fins are located below the third fixed plate 103. In this embodiment, the second heat sink fins and the first heat sink fins are both located between the first fixed plate 101 and the third fixed plate 103. Figure 1 As shown, a plurality of heat dissipation holes are provided on the side wall of the first shell 1001 of the overall device, and the first heat dissipation fins and the second heat dissipation fins follow the rotation of the turntable to discharge heat to the outside through the heat dissipation holes.

[0054] In one embodiment, Figure 15 As shown, the protein blotting processing device also includes a liftable liquid pipetting needle 401, and a first liquid pipetting channel is provided on the liquid pipetting needle 401, wherein a first fixed block 402 is provided at one end of the liquid pipetting needle 401, and three independent first sub-channels 4021, second sub-channel 4022 and third sub-channel 4023 are provided on the first fixed block 402, and the first sub-channel 4021, the second sub-channel 4022 and the third sub-channel 4023 are all connected to the first liquid pipetting channel.

[0055] In one embodiment, a cleaning tank 10310 is provided on the first bracket 10271. The cleaning tank 10310 includes a first cleaning hole 103101 and a second cleaning hole 103102. Figure 8As shown, the inner diameter of the first cleaning hole 103101 is larger than that of the second cleaning hole 103102, and the depth of the first cleaning hole 103101 is larger than that of the second cleaning hole 103102.

[0056] During the cleaning process of the pipette, the first cleaning hole 103101 is used for cleaning the first liquid suction channel of the pipette 401, and when the outer wall of the pipette 401 is cleaned, the pipette 401 is moved into the second cleaning hole 103102 for cleaning. By virtue of the smaller inner diameter of the second cleaning hole 103102, the inner wall of the second cleaning hole 103102 is impacted by water pressure, so as to reflect to the outer wall of the pipette 401 and clean the outer wall.

[0057] In an embodiment, in order to simplify the movement control of the pipette 401, only reciprocating lifting movement of the pipette 401 is allowed, and the center axes of the plurality of first mounting holes, the first cleaning hole 103101 and the second cleaning hole 103102 are located on the same circumferential line 1020, as shown in Figure 4 As shown, the projection of the pipette 401 on the plane of the second fixed disc 102 is located on the circumferential line 1020, so that only rotation of the disc is required, and the pipette 401 is lifted and moved, so as to accurately realize the conveying of the pipette 401.

[0058] Further, in an embodiment, as shown in Figure 16 , Figure 17 and Figure 20 , the waste liquid pump 501, the pure water pump 502 and the blocking liquid pump 503 are further arranged on the base. The waste liquid pump 501 is in communication with the first sub-channel 4021 through the first valve 5011, the pure water pump 502 is in communication with the second sub-channel 4022 through the second valve 5021, and the blocking liquid pump 503 is in communication with the third sub-channel 4023 through the third valve 5031. One end of the waste liquid pump 501 is further in communication with the waste liquid barrel 601 through a pipeline, and the waste liquid pump 501 is used for extracting the waste liquid generated during cleaning in the incubation box 10284 and recycling the waste liquid into the waste liquid barrel 601. One end of the pure water pump 502 is in communication with the external pure water barrel 602, and the pure water pump 502 is used for cleaning the pipette 401 and the incubation box 10284 for the next experiment, so as to avoid cross contamination. The blocking liquid pump 503 is further in communication with the external blocking liquid barrel 603, and the blocking liquid pump 503 is used for extracting the blocking liquid and conveying the blocking liquid into the clean incubation box 10284 before antibody incubation. It should be noted that the connection between the above-mentioned modules is realized by a hose, which will not be described here. The first valve 5011, the second valve 5021 and the third valve 5031 can be selected as electromagnetic valves to realize automatic control. The electromagnetic valve is a prior art, which will not be described here.

[0059] Furthermore, in order to simplify the connection structure and realize the absorption and dripping of the antibody reagent, Figure 21 As shown, a plunger pump 409 is disposed between the second valve 5021 and the second subchannel 4022. The inlet and outlet of the plunger pump 409 are connected to the second valve 5021 and the second subchannel 4022, respectively, via flexible pipes. Placing the plunger pump 409 in the pure water pipeline effectively prevents cross-contamination and ensures the accuracy of experimental data. Furthermore, since the amount of antibody reagent drawn is relatively small, it is unlikely to be drawn into the pure water bucket 602. When drawing or adding the antibody reagent, the plunger pump 409 performs suction or exhaust operations to complete the aforementioned operations.

[0060] In one embodiment, Figure 17 As shown, in order to avoid the pipette needle 401 from hitting the needle, the lifting component of the liftable pipette needle 401 includes a first connecting tube 403, a first lifting block 404, a first lifting block 405 and a second lifting block 4072. The second lifting block 404 is fixedly connected to the first lifting block 405. A preset distance is provided between the second lifting block 4072 and the first lifting block 404. One end of the first connecting tube 403 is connected to the first fixed block 402, and the other end of the first connecting tube 403 is connected to the first lifting block 404. The first lifting block 404 is arranged on the first lifting block 405, wherein the first lifting block 404 and the first lifting block 405 are separable, and the moving directions of the first lifting block 404, the second lifting block 4072 and the first lifting block 405 are consistent, and the central axis of the first connecting tube 403 is parallel to the central axis of the pipette needle 401. The three hoses connected to the first sub-channel 4021, the second sub-channel 4022 and the third sub-channel 4023 are all connected to the first valve 5011, the second valve 5021 and the third valve 5031 through the first channel 4031 in the first connecting pipe 403 and the first wiring channel 4041 set on the first lifting block 404. Of course, it can be known that the hoses between the first lifting block 404 and the first valve 5011, the second valve 5021 and the third valve 5031 are set to be of sufficient length to meet the reciprocating lifting and lowering requirements of the first lifting block 404.

[0061] Furthermore, if Figure 18As shown, to ensure stable lifting movement of the first lifting block 404 and the second lifting block 4072, a first guide rod 408 and a first connecting rod 410 are arranged parallel to each other. A first guide sleeve 4081 is provided on the first guide rod 408. The first guide sleeve 4081 can move relative to the first guide rod 408 and is connected to the first lifting block 404. In this embodiment, there are two first guide rods 408 and two first guide sleeves 4081. A through hole is provided on the first lifting block 404 to accommodate the first connecting rod 410. The first connecting rod 410 is movably inserted into the through hole. One end of the first connecting rod 410 is connected to the second lifting rod 4072, and the other end of the first connecting rod passes through the through hole in the first lifting block 404 and is connected to the first lifting block 405. There are two first connecting rods 410, which are arranged parallel to the first guide rod 408. When the second lifting block 4072 moves downward, the first lifting block 405 moves downward synchronously. Due to its own gravity, the first lifting block 404 moves downward along the length direction of the first guide rod 408; when the pipette needle 401 touches the bottom, the second lifting block 4072 can continue to drive the first lifting block 405 to move downward. Since the pipette needle 401 touches the bottom, the first lifting block 404 and the first lifting block 405 separate and remain stationary, thereby preventing the pipette needle 401 from hitting the needle, thereby protecting the pipette needle 401 and the entire device.

[0062] In this embodiment, the up and down reciprocating movement of the second lifting block 4072 is mainly reflected by a screw nut structure, specifically, it includes a first support 400 set on the base 1000, a first motor 407 is set on the first support 400, a first screw rod 4071 is set at the output end of the first motor 407, the first screw rod 4071 is rotatably set on the first support 400 through a bearing, the first screw rod 4071 is set parallel to the first guide rod 408, a first nut is set on the second lifting block 4072, and the first screw rod 4071 is set on the output end of the first motor 407. The second lifting block 4072 is movably passed through the first guide rod 408 for limiting, thereby combining with the first nut and the first screw rod 4071, and realizing the reciprocating movement of the second lifting block 4072 through the forward and reverse rotation of the first screw rod 4071. It should be pointed out that the other end of the first screw rod 4071 is set in suspension, and corresponding clearance holes are set on the first lifting block 404 and the first lifting block 405, so as to ensure that the other end of the first screw rod 4071 can avoid contact with the first lifting block 404 and the first lifting block 405.

[0063] Optionally, the lifting structure of the second lifting block 4072 may also be a synchronous belt and synchronous wheel structure, which can also realize the reciprocating lifting movement of the second lifting block 4072.

[0064] In one embodiment, since the turntable needs to rotate, in this application, a driving device is used for driving, which can realize the rotation of the turntable and the overturning of the reaction tank 1028 on the second fixed disc 102 at the same time. Specifically, as shown in Figure 13 and 14 The driving device of the turntable includes a second motor 301 arranged on the base 1000, a first driving sprocket arranged at the output end of the second motor 301, and a first driven sprocket 303 rotatably arranged on the limiting column 200 through a bearing, wherein the first fixed disc 101 is fixedly arranged on the first driven sprocket 303, the first fixed disc 101 rotates synchronously with the first driven sprocket 303, a first synchronous belt 302 is arranged between the first driving sprocket and the first driven sprocket 303, thereby realizing power transmission. The forward and reverse rotation of the turntable is realized by the forward and reverse rotation of the second motor 301.

[0065] Optionally, the transmission structure of the first driving sprocket and the first driven sprocket 303 can be replaced by a gear assembly. It can be known that the power driving structure and composition of the turntable are not limited by the present application, as long as the rotation of the turntable can be realized.

[0066] In one embodiment, since the refrigeration needs to provide electric energy, and the first refrigeration fin 10318 and the second refrigeration fin 10324 are both moved relative to the limiting column, a conductive slip ring 1014 arranged on the limiting column 200 and a conductive carbon brush 1013 arranged on the first fixed disc 101 are further included. The conductive carbon brush 1013 is fixedly arranged on the first fixed disc 101 through a carbon brush support 1011, the conductive carbon brush 1013 and the conductive slip ring 1014 realize sliding electrical contact, and can realize stable transmission of current and signal. It is prior art and will not be described in detail here. The limiting column 200 is internally provided with a first wire channel 2010, and a wire hole 20101 is formed on the side. The external wire is electrically connected with the conductive slip ring 1014 through the first wire channel 2010 and the wire hole 20101, and the conductive carbon brush 1013 is electrically connected with the first refrigeration fin 10318 and the second refrigeration fin 10324 through a wire. Since the position of the conductive carbon brush 1011 relative to the first fixed disc 101 is unchanged and rotates synchronously with the turntable, the problem of wire winding can be effectively avoided during the rotation of the turntable.

[0067] The above only describes the preferred embodiments of the present application. It should be noted that those skilled in the art can make several improvements and refinements without departing from the principles of the present application. These improvements and refinements should also be considered within the scope of protection of the present application.

Claims

1. A protein blotting processing device, characterized in that include: base; A limiting column, one end of which is fixedly disposed on the base, the limiting column having a preset length, and an end of the limiting column away from the base having a fixed cam component, the cam component having at least one concave portion and / or one convex portion; The turntable is rotatably arranged relative to the limiting column, and the turntable includes a first fixed plate and a second fixed plate, the first fixed plate is arranged close to the base, and the second fixed plate is arranged close to the cam component. The cam component is located between the first fixed plate and the second fixed plate, and a preset distance is set between the cam component and the second fixed plate. at least one first push rod, the first push rod being movably disposed on the lower side of the second fixed plate, one end of the first push rod being in contact with the cam component, and the first push rod being reciprocatingly movable along its own axis; The reaction tank is movably arranged on the upper side of the second fixed plate. The reaction tank is provided with a first connecting portion. The second fixed plate is provided with a through first slot. The first connecting portion passes through the first slot and is movably connected to the other end of the first push rod. The first push rod can drive the reaction tank to swing back and forth relative to the second fixed plate.

2. A protein blotting processing device according to claim 1, characterized in that, A first roller is provided at the contact end of the first push rod and the cam component. The first roller is rotatably arranged on the first push rod, and the first roller can abut against the cam component.

3. A protein blotting processing device according to claim 1, characterized in that: The concave portion and the convex portion are both cambered structures, and the convex portion and the concave portion are connected by a smooth transition.

4. A protein blotting processing device according to claim 1, characterized in that: A first sliding groove is provided on the lower side of the second fixed plate, the first push rod is movably arranged on the first sliding groove, and a plurality of second rollers are provided on both sides of the first sliding groove, and the plurality of second rollers are all in contact with the side surface of the first push rod.

5. A protein blotting processing device according to claim 2, characterized in that: It also includes a first return spring, one end of which is connected to the outside of the first sliding groove, and the other end of the first return spring is connected to the first push rod.

6. A protein blotting processing device according to claim 1, characterized in that: An adapted incubation box is provided in the reaction tank, and the incubation box is detachably arranged on the reaction tank.

7. A protein blotting processing device according to claim 6, characterized in that: It also includes a third fixing plate, the third fixing plate is arranged between the first fixing plate and the second fixing plate, and the third fixing plate is connected to the first fixing plate and the second fixing plate respectively; Among them, several test tube assemblies are provided on the second fixed plate and the third fixed plate, and the test tube assembly includes a first bracket provided on the second fixed plate and an insulation chamber provided on the third fixed plate, and a plurality of first mounting holes are provided on the first bracket, and the first mounting holes are connected to the insulation chamber, and a first adapter and a first cooling plate are provided in the insulation chamber, and the first adapter is used to support the bottom of the test tube, and the first cooling plate is used for cooling the insulation chamber.

8. A protein blotting processing device according to claim 7, characterized in that: A first radiator and a temperature monitoring sensor are further provided on the outside of the heat preservation cavity. The first radiator includes a plurality of first heat dissipation fins spaced apart from each other. The temperature monitoring sensor is used to detect the temperature in the heat preservation cavity.

9. A protein blotting processing device according to claim 7, characterized in that: The first bracket is further provided with a cleaning groove, which includes a first cleaning hole and a second cleaning hole. The inner diameter of the first cleaning hole is larger than the inner diameter of the second cleaning hole, and the depth of the first cleaning hole is larger than the depth of the second cleaning hole.

10. The protein blotting processing device according to claim 9, characterized in that: The central axes of the plurality of first mounting holes, the first cleaning holes, and the second cleaning holes are all located on the same circumferential line.

11. A protein blotting processing device according to claim 10, characterized in that: The first supports and the reaction tanks are alternately arranged on the second fixed plate.

12. A protein blotting processing device according to claim 11, characterized in that: It also includes a second refrigeration fin and a refrigeration base, wherein the second refrigeration fin is fixed relative to the third fixed plate, and the second refrigeration fin is connected to the refrigeration base; Wherein, the reaction tank is provided with a second notch, a refrigeration contact seat is provided at the second notch, the refrigeration contact seat is in contact with the incubation box, and one end of the refrigeration seat abuts against the refrigeration contact seat.

13. A protein blotting processing device according to claim 12, characterized in that: A smooth guide structure is provided between the refrigeration contact seat and the refrigeration seat, and the smooth guide structure includes a first arc-shaped groove provided on the refrigeration contact seat and a first arc-shaped boss provided at one end of the refrigeration seat, and the first arc-shaped groove is adapted to the first arc-shaped boss, wherein the rotation axis of the refrigeration contact seat relative to the second fixed disk coincides with the center of the first arc-shaped groove.

14. A protein blotting processing device according to claim 13, characterized in that: The third fixed plate is also provided with a first thermal insulation seat, and the first thermal insulation seat is provided with a through first refrigeration channel. The refrigeration seat passes through the first refrigeration channel and abuts against the refrigeration contact seat. The first thermal insulation seat is provided with a first clearance groove, and the position of the first clearance groove corresponds to the position of the first connecting part. The first push rod can be extended into the first clearance groove and connected to the first connecting part.

15. A protein blotting processing device according to claim 14, characterized in that: A second heat sink is provided on the first heat insulation seat. The second heat sink includes a plurality of second heat dissipation fins arranged in parallel. The second heat dissipation fins are located on the lower side of the third fixing plate.

16. A protein blotting processing device according to any one of claims 1 to 15, characterized in that: It also includes a liftable liquid-absorbing needle, which is provided with a first liquid-absorbing channel. A first fixed block is provided at one end of the liquid-absorbing needle, and a first sub-channel, a second sub-channel and a third sub-channel are provided on the first fixed block. The first sub-channel, the second sub-channel and the third sub-channel are all connected to the first liquid-absorbing channel.

17. A protein blotting processing device according to claim 16, characterized in that: It also includes a waste liquid pump, a pure water pump and a closed liquid pump arranged on the base, wherein the waste liquid pump is connected to the first sub-channel through a first valve, the pure water pump is connected to the second sub-channel through a second valve, and the closed liquid pump is connected to the third sub-channel through a third valve.

18. A protein blotting processing device according to claim 17, characterized in that: A plunger pump is provided between the second valve and the second sub-channel, and the plunger pump is connected in series with the second valve.

19. The protein blotting processing device according to claim 16, characterized in that: It also includes a first connecting tube, a first lifting block, a first supporting block and a second lifting block, the second lifting block is connected to the first supporting block, and a preset distance is provided between the second lifting block and the first lifting block, one end of the first connecting tube is connected to the first fixed block, and the other end of the first connecting tube is connected to the first lifting block, the first lifting block is arranged on the first supporting block, and the first lifting block can be detached from the first supporting block; wherein, the moving directions of the first lifting block, the first supporting block and the second lifting block are consistent, and the central axis of the first connecting tube is parallel to the central axis of the aspiration needle.

20. The protein blotting processing device according to claim 19, characterized in that: It also includes a first connecting rod and a first guide rod arranged parallel to each other, a first guide sleeve is provided on the first guide rod, the first guide sleeve is connected to the first lifting block, the first lifting block is movably passed through the first guide rod, and the first connecting rod passes through the first lifting block and is connected to the first lifting block.

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