Natural component composition with skin micro-ecology conditioning function and application of natural component composition in skin care products for children as well as preparation method of natural component composition

Abstract

The invention discloses a natural component composition for conditioning skin micro-ecology, a preparation method of the natural component composition and application of the natural component composition in skin care products for children. The composition comprises a synergistic combination of a sphingomonas fermentation product extract, a haematococcus pluvialis extract rich in astaxanthin, an abelmoschus esculentus fruit extract rich in polysaccharides, and a yeast / zinc fermentation product. The present invention provides improvements in astaxanthin in terms of chemical stability and transdermal permeability. The composition aims to enhance the immature skin barrier, provide a good effect, and support the healthy development of skin microecology, thereby solving the unique physiological needs of children's skin. The invention also describes a preparation method of the composition and application of the composition in cosmetics for children and dermatological products.

Description

Technical Field

[0001] The present invention relates to the fields of cosmetics and dermatology. More specifically, it relates to novel topical compositions comprising natural and fermented ingredients. The invention further relates to methods for stabilizing highly effective but unstable antioxidant compounds, such as astaxanthin, in cosmetic formulations and enhancing their delivery to the skin. The invention has particular application in the formulation of skin care products designed for the unique structural and physiological characteristics of children's skin, including infants and young children. Background Art

[0002] The skin is the first barrier between the human body and the external environment. There is also an ecosystem at the interface between the skin and the environment - the skin microecology. The skin microecology refers to the microbial community existing on the surface of the skin and the ecosystem formed by it and the environment on the surface of the skin, which plays a unique biological function. These microorganisms include bacteria, fungi, viruses, etc., which together with the skin form a complex ecosystem. The interaction between skin microorganisms, the host (skin) and the external environment constitutes the balance of the skin microecology, which is crucial to skin health. The balance of the skin microecology plays an important role in protecting the skin barrier, skin metabolism and regulating immune function.

[0003] The skin microbiome is a crucial component of this balanced system. Under normal circumstances, skin microbes work together to defend against the invasion of harmful external substances by competing for nutrients, secreting antimicrobial substances, and regulating skin pH. Secondly, skin microbes secrete triglycerides from sebum and produce free fatty acids, which provide moisture to the skin and maintain an acidic environment, inhibiting the growth of harmful bacteria. They also synthesize vitamin K and B vitamins for normal skin metabolism and renewal, and participate in metabolic processes such as stratum corneum renewal and collagen degradation and synthesis. Finally, skin microbes can regulate the intensity and type of immune responses, stimulating the development and maturation of the skin's immune system. Studies have shown that an imbalance in the skin's microbiome is closely linked to the occurrence and progression of various skin diseases. When harmful bacteria dominate, the microbiome is disrupted, potentially leading to skin problems such as dryness, allergies, dermatitis, acne, and rashes. Therefore, the skin microbiome is the first line of defense for the human skin and a crucial foundation for skin health.

[0004] The stratum corneum (SC), the outermost layer of the skin, primarily functions as a natural barrier by preventing microorganisms, allergens, and irritants from entering the body. The average thickness of a child's stratum corneum is approximately 7 μm, 30% thinner than the 10 μm of an adult's. The epidermis is also 20% thinner. This structural characteristic results in the skin's barrier function being less mature, making it more susceptible to external stimuli. Furthermore, children's skin has lower levels of natural moisturizing factor (NMF) and sebum secretion from sebaceous glands, leading to faster water loss and increased skin dryness. Children's skin also has lower melanin content, making them less resistant to UV rays and more susceptible to sunburn. The pH of children's skin is close to neutral (6.6-7.7), which is not conducive to the growth of beneficial bacteria and even creates favorable conditions for the colonization and reproduction of certain pathogens.

[0005] Children's unique physiological characteristics collectively shape a skin microecological environment that is distinct from that of adults. Scientific research has revealed that the microbiome of children's skin has distinct characteristics in species composition and abundance. Unlike adult skin, which is dominated by lipophilic Corynebacterium and Propionibacterium (now called Cutibacterium), the microbiome of children's skin is mainly composed of genera such as Streptococcus, Granulicatella, and Haemophilus. In terms of fungi, Candida is dominant on children's skin, while Malassezia is dominant on adults. This microbial community, dominated by Streptococci, has relatively weak competitive inhibition ability when faced with invasion by opportunistic pathogens, especially Staphylococcus aureus. Staphylococcus aureus is a key pathogen causing skin infections and exacerbating inflammatory skin diseases such as atopic dermatitis (AD) in children. Children's skin's higher pH, imperfect physical barrier, and unique immune status create a vulnerable system prone to microbial dysbiosis, often referred to as "children's skin dysbiosis." This dysbiosis is not only the root cause of skin sensitivity, dryness, and redness, but also a key contributor to the development and progression of chronic inflammatory skin diseases like atopic dermatitis. Therefore, the core technical challenge of children's skin care lies not in simply providing "gentle" or "moisturizing" products, but rather in developing solutions that proactively intervene in and precisely regulate this specific microbial ecosystem. Such solutions must selectively inhibit the growth of harmful bacteria (such as S. aureus) while simultaneously protecting and supporting the growth of beneficial bacteria (such as Staphylococcus epidermidis and the streptococcal flora inherent to children's skin), thereby rebuilding and maintaining the robustness of the skin's microbial barrier.

[0006] With a deeper understanding of the skin microbiome, technological innovations driven by the skin microbiome are quietly evolving. Faced with the multi-billion dollar market potential of precision skincare, increasing attention is being directed towards the development of targeted cosmetic products based on skin microbiome research. However, the vast majority of these products are developed for adult skin, with formulations based on adult skin physiology and microbial characteristics. For example, core fermentation ingredients from brands like Estée Lauder and Lancôme, such as bifid yeast fermentation lysate (derived from Bifidobacterium bifidum) or Lactobacillus fermentation products, are intended to support the adult skin microbiome or provide anti-aging and repair benefits. These products fail to account for the unique environment of children's skin, which is dominated by Streptococcus and has low sebum secretion. Direct application to children's skin may not achieve the desired microbiome-modulating effects and may even result in adverse reactions due to incompatibility. The formulation concepts of children's skincare products on the market remain fundamentally based on "safety" and "moisturizing." They typically use highly occlusive ingredients like petrolatum and mineral oil, or single humectants like glycerin and hyaluronic acid, aiming to reduce water loss and external irritation by forming a physical protective film. This type of care is passive and can only provide temporary barrier support, but lacks the ability to actively regulate the skin's microecology and strengthen the skin's own defenses from the root. They cannot solve recurring skin problems caused by microecological imbalance.

[0007] Astaxanthin is a natural carotenoid extracted primarily from microalgae such as Haematococcus pluvialis. It has attracted much attention in the skincare field for its antioxidant properties. Scientific research has shown that astaxanthin's antioxidant activity is hundreds of times that of vitamin E and several times that of beta-carotene, earning it the nickname "super antioxidant." It can effectively neutralize free radicals generated by ultraviolet rays, environmental pollution, and other factors, and has shown great potential in combating photoaging, soothing inflammation, and protecting skin cells. However, the commercial application of astaxanthin has always faced two core technical bottlenecks. First, the instability of its chemical structure. The long conjugated polyene chain structure contained in the astaxanthin molecule makes it extremely sensitive to light, heat, and oxygen. It is easily oxidized and degraded under conventional cosmetic formulations and storage conditions, causing its iconic red color to fade and lose its biological activity. Although existing technologies attempt to improve its stability through delivery systems such as microencapsulation, liposome preparation, and nanoemulsion, these methods are often complex and costly, or may destroy the delicate structure of astaxanthin during the preparation process (such as high-pressure homogenization and ultrasonic treatment), resulting in reduced activity. Second, transdermal absorption efficiency is low. Astaxanthin is a large molecular and highly lipophilic compound, which makes it difficult to penetrate the stratum corneum, which is the main skin barrier. According to the "Lipinski Five Rules" of pharmacokinetics, such molecules are generally poorly absorbed orally and have weak skin permeability, resulting in extremely low bioavailability when applied externally. Most of the active ingredients are blocked on the surface of the skin and cannot reach the dermis to exert their deep antioxidant and repair effects.

[0008] In summary, the existing technology lacks a technical solution that can safely and effectively incorporate potent but highly unstable natural active ingredients, such as astaxanthin, into children's skincare products. The market urgently needs an innovative composition that not only overcomes the stability and permeability challenges of astaxanthin, but also must be naturally derived, mildly formulated, and synergistically regulate the unique microecological environment of children's skin, thereby providing a comprehensive and safe skin health solution. Summary of the Invention

[0009] The primary purpose of the present invention is to overcome the shortcomings of the existing technology and provide a natural, safe and efficient cosmetic composition, aiming to solve the problem of effectively protecting the highly unstable lipophilic active ingredient astaxanthin in water-based cosmetic systems, preventing it from degradation and failure due to environmental factors such as light, heat, and oxygen, and significantly improving the transdermal absorption efficiency of large-molecule, highly lipophilic astaxanthin, so that it can effectively penetrate the delicate skin barrier of children and reach the target site of action. A composition is developed that can gently regulate the microecological balance of children's skin and enhance the skin's own biological barrier function, ensuring that the composition meets the high safety standards of children's cosmetics, has low allergenicity, and is suitable for the sensitive skin of children aged 12 and under.

[0010] Another object of the present invention is to provide a method for preparing the natural composition, in particular an innovative preparation process for one of the key components.

[0011] To achieve the above object, the present invention provides the following technical solutions:

[0012] Provided is a cosmetic composition for conditioning skin microecology, comprising the following components in parts by weight:

[0013] (a) Sphingomonas fermentation product extract: 0.1-10.0 parts;

[0014] (b) Haematococcus pluvialis extract: 0.05 parts to 5.0 parts;

[0015] (c) Ambrette coffea fruit extract: 0.5-15.0 parts;

[0016] (d) Yeast / zinc fermentation product: 0.1 part to 8.0 parts.

[0017] In some embodiments, the weight proportions of the components are: (a) Sphingomonas fermentation product extract: 0.5 parts to 5.0 parts; (b) Haematococcus pluvialis extract: 0.1 parts to 2.0 parts; (c) Ambrette coffee fruit extract: 1.0 parts to 10.0 parts; (d) yeast / zinc fermentation product: 0.5 parts to 5.0 parts.

[0018] In some embodiments, the Sphingomonas fermentation product extract is obtained by fermenting in a culture medium with a carbon-nitrogen ratio greater than 40:1, and then purifying by alcohol precipitation and membrane separation technology.

[0019] In some embodiments, the product is used to prepare a children's skin care product, and the product dosage form is selected from oil-in-water (O / W) emulsion, cream, essence or gel.

[0020] Provided is the use of the composition in preparing a product for improving the stability of astaxanthin in a cosmetic matrix.

[0021] Provided is the use of the composition in preparing a skin external use product for enhancing the transdermal absorption efficiency of astaxanthin.

[0022] Provided is the use of the composition in preparing a hypoallergenic children's skin care product that regulates the microecological balance of children's skin, promotes the relative abundance of Staphylococcus epidermidis, and maintains microbial diversity.

[0023] Provided is a method for improving children's skin condition, comprising the step of topically applying any of the above compositions to the skin.

[0024] A method for increasing the stability of astaxanthin in a topical formulation is provided, comprising the step of combining astaxanthin with a synergistic mixture of Sphingomonas ferment extract, Ambrette fruit extract, and Saccharomyces / zinc ferment.

[0025] Provided is a method for preparing any of the above compositions, comprising adding astaxanthin-containing Haematococcus pluvialis extract, Sphingomonas fermentation product extract, Ambrette coffee fruit extract, and yeast / zinc fermentation product during the step of cooling the emulsion to below 40°C.

[0026] Compared with the prior art, the beneficial effects of the present invention are as follows: by utilizing the physicochemical interactions between the four components and combining them in an optimal ratio, a protective effect can be exerted on astaxanthin and the transdermal efficiency of astaxanthin can be increased. In this way, most of the astaxanthin can reach the dermis of the skin and exert deep antioxidant and repair effects, thereby producing a synergistic effect of 1+1+1+1>4. At the same time, the components in the cosmetic composition are of natural origin and are non-irritating. Therefore, the stability and permeability problems of astaxanthin can be overcome, and it is natural, mild in formula, and can synergistically regulate the unique microecological environment of children's skin, thereby providing an overall solution for the "microecological-barrier axis". It systematically improves the health level and self-resistance of children's skin through the trinity of "cultivating bacteria", "strengthening the screen" and "protection", which is fully in line with the advanced concepts of modern pediatric skin care and the dual high requirements of children's cosmetics regulations for safety and efficacy. DETAILED DESCRIPTION

[0027] To make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be described clearly and completely below. The described embodiments are only part of the embodiments of the present invention, not all of them.

[0028] Therefore, the detailed description of the embodiments of the present invention provided below is not intended to limit the scope of the claimed invention, but rather merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present invention.

[0029] The present invention provides a cosmetic composition for regulating skin microecology, which comprises the following components in scientific proportions and capable of producing a synergistic effect, measured by weight: 0.1-10.0 parts of a Sphingomonas fermentation product extract; 0.05-5.0 parts of a Haematococcus pluvialis extract; 0.5-15.0 parts of an Ambrette coffee fruit extract; and 0.1-8.0 parts of a yeast / zinc fermentation product.

[0030] Specifically, the amount of Sphingomonas fermentation product extract can be set at 0.1 parts, 5.05 parts, or 10.0 parts; the amount of Haematococcus pluvialis extract can be set at 0.05 parts, 2.5 parts, or 5.0 parts; the amount of Ambrette coffee fruit extract can be set at 0.5 parts, 7.75 parts, or 15.0 parts; and the amount of yeast / zinc fermentation product can be set at 0.1 parts, 4.05 parts, or 8.0 parts.

[0031] In a preferred embodiment, the weight proportions of the components are: Sphingomonas fermentation product extract: 0.5-5.0 parts; Haematococcus pluvialis extract: 0.1-2.0 parts; Ambrette coffee fruit extract: 1.0-10.0 parts; yeast / zinc fermentation product: 0.5-5.0 parts.

[0032] Specifically, the amount of Sphingomonas fermentation product extract can be set to 0.5 parts, 2.75 parts, or 5.0 parts; the amount of Haematococcus pluvialis extract can be set to 0.1 parts, 1.05 parts, or 2.0 parts; the amount of Ambrette coffee fruit extract can be set to 1.0 parts, 5.5 parts, or 10.0 parts; and the amount of yeast / zinc fermentation product can be set to 0.5 parts, 2.75 parts, or 5.0 parts.

[0033] Compared with the prior art, the composition disclosed in the present invention achieves unexpected technical effects through the exquisite combination of four natural components, which are specifically reflected in:

[0034] Precisely regulates children's skin microbiome: The Sphingomonas ferment extract and yeast / zinc ferment in this combination act as "postbiotics," providing beneficial metabolites to the skin microbiome. Experiments have shown that this combination selectively supports the growth of beneficial skin bacteria, such as Staphylococcus epidermidis, without negatively impacting the overall balance of the skin's commensal flora. This helps maintain and restore the health and stability of children's skin microbiome, fundamentally strengthening the skin's biological barrier function and reducing the risk of sensitivity and inflammation.

[0035] High safety and child-friendly: The four core active ingredients used in this invention are all derived from natural plant extraction or biofermentation technology, and have good biocompatibility and safety. The formula design strictly adheres to the core concept of "safety first, efficacy required, and minimalist formula" in China's "Technical Guidelines for Children's Cosmetics." Through rigorous safety assessments, including the human repetitive injury patch test (HRIPT) and the in vitro eye irritation test (BCOP), it is proven that this composition has extremely low irritation and sensitization risks, and fully meets the stringent safety requirements for the delicate skin of children aged 12 and under.

[0036] Synergistically Enhanced Stability and Permeability: This invention doesn't simply blend multiple ingredients; instead, it leverages their physicochemical interactions to create a synergistic effect of 1+1+1+1 > 4. On the one hand, the natural polysaccharides in the coffee sunflower fruit extract and the biopolymers in the Sphingomonas fermentation product form a three-dimensional network-like "bioshield" structure in the aqueous phase. This structure physically encapsulates and suspends the oil droplets encapsulating astaxanthin, effectively shielding them from external damaging factors like light and oxygen. Its stability far exceeds that of either polysaccharide or polymer alone. On the other hand, the Sphingomonas fermentation product is rich in sphingolipids, whose structure is similar to the skin's own ceramides. These sphingolipids act as highly effective natural penetration enhancers, temporarily increasing the fluidity of stratum corneum lipids. Simultaneously, the yeast / zinc fermentation product biotransforms into a large number of readily absorbable small-molecule metabolites, which synergize with the sphingolipid components to create a "dual-channel permeation enhancement system," significantly enhancing the transdermal efficiency of the entire efficacy complex (particularly the large-molecule astaxanthin).

[0037] The detailed description of each component used in the composition of the present invention is as follows:

[0038] The Sphingomonas fermentation product in the present invention is not a simple Sphingomonas fermentation product, but is obtained through an optimized fermentation and purification process to maximize the acquisition of high-molecular-weight, structurally pure exopolysaccharides (EPS). This polysaccharide is also called sphingan. It is not ceramide, but it can command the skin to produce ceramide itself, significantly improving the activity of a variety of key lipid synthases, thereby accelerating and increasing the production of endogenous barrier lipids such as ceramide and cholesterol required by the skin itself. This EPS is the key skeleton material for the subsequent construction of the astaxanthin stabilization system. This process complies with the principles of green chemistry, and the product is natural and biodegradable. The Sphingomonas fermentation product extract can be used as a natural, mild penetration enhancer. By interacting with the stratum corneum lipids, it temporarily increases their fluidity and opens channels for the penetration of macromolecular active substances such as astaxanthin.

[0039] Haematococcus pluvialis is a freshwater single-celled microalgae that accumulates large amounts of astaxanthin in extreme environments (such as strong light, high salt, and nutrient deficiency). The content of astaxanthin can reach 1.5%-3% of the dry weight. Its antioxidant activity is approximately 6,000 times that of vitamin C and 550 times that of vitamin E. It is one of the strongest natural antioxidants known in nature. Its unique "transmembrane antioxidant" ability can simultaneously protect the inner and outer layers of cell membranes from oxidative damage, inhibit lipid peroxidation, and protect skin cells from oxidative stress damage caused by environmental factors such as ultraviolet (UV) radiation, and inhibit inflammatory responses.

[0040] The coffee sunflower fruit extract is derived from renewable plants, so it is green, economical and environmentally friendly, has good biodegradability and low irritation. The extract is rich in viscous polysaccharides and effectively removes impurities such as proteins and pigments. Coffee sunflower polysaccharide has excellent film-forming and emulsification stability. In the present invention, it synergizes with the biopolymers in the extract of Sphingomonas fermentation products to form a flexible, dense and moisturizing biofilm network in the aqueous phase. This network can physically encapsulate and fix the tiny oil droplets that encapsulate astaxanthin, like a "biological shield", greatly reducing the contact of astaxanthin with external light, heat and oxygen, thereby significantly improving its stability in the entire formula system. This synergistic stabilization mechanism is one of the key innovations of the present invention.

[0041] Saccharomyces / Zinc Ferment is produced by fermenting Saccharomyces cerevisiae in a zinc-rich medium. This biofermentation process organically binds zinc to yeast-derived peptides and metabolites, significantly increasing its bioavailability to the skin compared to simple zinc salts. Saccharomyces / Zinc Ferment is the primary soothing and anti-inflammatory agent. It directly targets the sensitivity of children's skin while promoting barrier repair and providing antioxidant support by providing bioavailable zinc. It works synergistically with Sphingomonas Ferment Extract to create a balanced skin microbiome.

[0042] In some embodiments, the cosmetic composition may be supplemented with some conventional carriers selected from emollients, emulsifiers, thickeners, moisturizers, preservatives, and pH adjusters.

[0043] In summary, the present application does not simply add and mix the four substances to make them work together, but rather utilizes the physical and chemical interactions between them and combines them in the optimal proportion, so that they can have a protective effect on the astaxanthin in the Haematococcus pluvialis extract and increase the transdermal efficiency of astaxanthin. In this way, most of the astaxanthin can reach the dermis of the skin and exert deep antioxidant and repair effects, thereby producing a good synergistic effect.

[0044] The present invention also provides a method for preparing the Sphingomonas fermentation product in the above composition, the method comprising the following steps:

[0045] S1: Select a selected Sphingomonas strain with high EPS production, such as Sphingomonaselodea, or a new strain isolated and optimized from the natural environment;

[0046] S2: Under sterile conditions, the bacteria are inoculated into a liquid culture medium with a carbon-nitrogen ratio (C / N ratio) of 50:1, and appropriate amounts of plant sterols and trace elements are added. Submerged aerobic fermentation is carried out in a fermenter. The key control parameters during the fermentation process are: constant temperature at 28-32°C, online monitoring by automatic feeding and pH electrode, precise maintenance of pH value between 6.8-7.2 using ammonia or sodium hydroxide, and ensuring that the dissolved oxygen concentration is not less than 20% by adjusting the stirring rate and ventilation volume. The fermentation cycle is approximately 72-96 hours, until the viscosity of the fermentation liquid reaches its peak;

[0047] S3: After fermentation, the fermentation broth is heated to 80-90°C and maintained for 15 minutes to inactivate enzymes that may degrade polysaccharides. Subsequently, the bacterial cells are removed using a high-speed centrifuge. The supernatant is taken, 2 volumes of pre-cooled isopropanol are added, and the EPS is allowed to settle. The precipitate is collected, redissolved with deionized water, and then diafiltration is performed through a tangential flow ultrafiltration system to effectively remove residual salts, pigments, and small molecular impurities in the culture medium;

[0048] S4: The purified EPS solution is freeze-dried to obtain a white or light yellow, high-purity powdered Sphingomonas fermentation product extract with an EPS content of no less than 90%. This EPS has excellent film-forming properties and emulsion stability, providing an ideal primary interface layer for encapsulating the lipophilic astaxanthin.

[0049] The present invention also provides a method for preparing a children's skin care product comprising the above-mentioned composition, in particular a method for preparing an oil-in-water (O / W) emulsion or cream, as well as specific applications of the composition in improving the stability of astaxanthin, enhancing its transdermal absorption and regulating the skin microecology.

[0050] Example:

[0051] The present invention will be further described below by way of specific examples, but the scope of protection of the present invention is not limited to these examples. In each example, unless otherwise specified, all raw materials used are commercially available cosmetic grade raw materials, and all percentages are by weight.

[0052] Examples 1-4 and Comparative Examples 1-4: Preparation of Children's Soothing Moisturizing Lotion / Cream

[0053] Emulsions or creams of Examples 1-4 (compositions of the present invention) and Comparative Examples 1-4 (for comparative verification) as shown in Table 1 were prepared.

[0054] Table 1: Components and contents (wt%) of Examples 1-4 and Comparative Examples 1-4

[0055]

[0056]

[0057] Preparation process:

[0058] The preparation was carried out according to the standard O / W (oil in water) emulsification process.

[0059] S1: Mix deionized water, humectants such as glycerin, and other water-soluble components, heat to 75-80°C, and stir until completely dissolved.

[0060] S2: Mix the emollient, emulsifier and other oil-soluble components, heat to 75-80°C, and stir until completely melted and mixed evenly.

[0061] S3: Slowly add the oil phase to the water phase under high shear homogenization conditions to form a stable, smooth emulsion. Continue homogenizing for a certain period of time to ensure uniform particle size.

[0062] S4: Stop heating and allow the emulsion to cool with gentle stirring. This is a critical step in preparation for adding heat-sensitive active ingredients.

[0063] S5: When the emulsion temperature drops below 40°C, add the heat-sensitive active ingredients in the following order: Haematococcus pluvialis extract, Sphingomonas ferment extract, Saccharomyces / zinc ferment, and Amla fruit extract. This low-temperature addition is crucial to preserve the bioactivity of astaxanthin and the ferment.

[0064] S6: Adjust the pH to a range suitable for children's skin (preferably pH 5.0-6.0) and add a suitable preservative system. Conduct final quality control testing, including viscosity, pH, sensory evaluation, and microbial counts, to ensure the consistency and safety of each batch of product.

[0065] Efficacy and safety testing

[0066] In order to verify the beneficial effects of the composition of the present invention, a series of efficacy and safety tests were conducted on the above examples and comparative examples.

[0067] 1. Evaluation of Astaxanthin Stability

[0068] The samples of Examples 1-2 and Comparative Examples 1-4 were separately packaged in transparent glass bottles. One group was placed in a 45°C constant temperature incubator to avoid light, and the other group was placed in an irradiation box equipped with a 365nm ultraviolet lamp (30 cm away from the sample) and continuously irradiated at room temperature. Sampling was performed on days 0, 7, 14, and 28. The astaxanthin content in the samples was determined by high performance liquid chromatography (HPLC). The chromatographic column was a C18 column, the mobile phase was a gradient elution of methanol / water / tert-butyl methyl ether, and the detection wavelength was 478 nm. The astaxanthin retention rate was calculated based on the measured concentration.

[0069] Astaxanthin retention rate $(%)=(C t / C0)\times 100%$

[0070] Among them, C t is the astaxanthin concentration at time t, and C0 is the initial (time 0) astaxanthin concentration.

[0071] Results: As shown in Table 2.

[0072] Table 2: Retention rate of astaxanthin in each sample under different conditions (%)

[0073]

[0074] Conclusion: The experimental results show that after heating at 45°C and irradiating with ultraviolet light for 28 days, the retention rates of astaxanthin in Examples 1 and 2 were significantly higher than those in the samples lacking the Sphingomonas fermentation product extract (Comparative Example 1) and the samples lacking the Coffea serrata fruit extract (Comparative Example 3) (p<0.01). This proves that the complex of the Sphingomonas fermentation product extract and the Coffea serrata fruit extract has a significant synergistic stabilizing effect on astaxanthin, that is, the effectiveness of the "polysaccharide bioshield" mechanism. The lack of yeast / zinc fermentation product (Comparative Example 4) has little effect on stability, indicating that its main function is not stability. Moreover, after heating at 45°C and irradiating with ultraviolet light for 28 days, the retention rates of astaxanthin in Example 1 were significantly higher than those in Example 2, proving that the ratio of Example 1 can make astaxanthin more stable.

[0075] 2. Evaluation of Skin Microecological Regulation

[0076] 30 healthy child volunteers aged 6-12 years were recruited. After signing the informed consent form, a 4-week randomized controlled study of their left and right half faces was conducted. The subjects used the product of Example 1 on one cheek twice a day, morning and evening, and a matrix lotion without the four core functional ingredients of Phase C on the other side (blank control). At week 0 (before use) and week 4 (after use), professionals used sterile cotton swabs to sample skin surface microorganisms in the same area (zygomatic bone) of both cheeks. The total DNA of the samples was extracted, and the V3-V4 hypervariable region of the bacterial 16S rRNA gene was amplified and high-throughput sequenced. The Alpha diversity index (Shannon index, reflecting the richness and uniformity of the flora) and the relative abundance of key genera were calculated through bioinformatics analysis.

[0077] Results: As shown in Table 3.

[0078] Table 3: Changes in relative abundance and alpha diversity of key bacterial genera on the skin after 4 weeks of use

[0079]

[0080] Conclusion: Compared with the blank control group, after continuous use of the product of Example 1 or Example 2 for 4 weeks, the relative abundance of the beneficial bacteria Staphylococcus epidermidis on the cheek skin of Example 1 increased significantly, and the overall diversity of the flora (Shannon index) was also maintained and improved, and the difference was statistically significant; the relative abundance of the beneficial bacteria Staphylococcus epidermidis on the cheek skin of Example 2 increased less, and although it was still better than the control, the significance was weaker, while the overall diversity of the flora (Shannon index) decreased slightly, with no statistical significance, and the positive regulation of the skin microecology existed but was weaker than that of Example 1. This shows that the present invention can positively regulate the skin microecology of children, promote the growth of beneficial bacteria, maintain the balance of the flora, thereby enhancing the biological barrier function of the skin, and Example 1 is better than Example 2.

[0081] 3. Safety Evaluation

[0082] Following the relevant OECD guidelines and industry standards, a third-party clinical testing organization was commissioned to conduct a study on 52 healthy adult volunteers (including 26 who reported having sensitive skin). Approximately 0.2g of the product (lotion) in Example 1 was applied to a closed patch and then applied to the back of the subject. The test was divided into three phases: an induction phase (9 applications, 3 times per week for 3 weeks), a rest phase (2 weeks), and a challenge phase (1 application each to the original site and a new site). A dermatologist evaluated the skin reaction 24 hours after each application, with a scoring scale of 0-4 points.

[0083] Following the OECD TG 437 test guidelines, an ex vivo bovine cornea was used as the test system. The product from Example 1 was placed in direct contact with the bovine cornea for a specific period of time. Changes in corneal opacity and the amount of sodium fluorescein permeation were measured to calculate an in vitro irritation score (IVIS) to assess the product's potential for eye irritation.

[0084] Results: As shown in Table 4.

[0085] Table 4: Summary of safety test results

[0086]

[0087] Conclusion: The product in Example 1 is non-irritating and non-sensitizing to human skin. It supports claims of "hypoallergenicity" and "dermatologist-tested." According to the GHS classification standard, IVIS ≤ 3, which is determined to be "non-irritating" (GHS No Category). This product poses no risk of eye irritation, demonstrating that the composition of the present invention and products made therefrom are highly safe, with extremely low risks of irritation and sensitization. They fully comply with the requirements of the "Regulations on the Supervision and Administration of Children's Cosmetics" and are suitable for daily skin care for children aged 12 and under.

[0088] It is understood that the cosmetic composition of the present application can also be prepared into gels, medical dressings, shampoos, etc., as long as they are cosmetics or external skin products. The specific selection is not limited here and is based on actual production needs. All of them can be prepared using conventional preparation methods, such as preparing a gel containing the cosmetic composition of the present application using conventional methods for preparing gels, and preparing a medical dressing containing the cosmetic composition of the present application using conventional methods for preparing medical dressings. These preparation methods are known to those skilled in the art and are achievable, and will not be described in detail here.

[0089] In addition, it can be understood that the invention name of this application can also be set as a cosmetic composition and its application and preparation method.

[0090] Although the present invention has been described with reference to the above specific embodiments, it will be apparent to those skilled in the art that many substitutions, modifications, and variations can be made based on the above. Therefore, all such substitutions, modifications, and variations are intended to be encompassed within the spirit and scope of the appended claims.

Claims

1. A cosmetic composition for conditioning skin microecology, characterized in that: By weight, it comprises the following components: (a) Sphingomonas fermentation product extract: 0.1-10.0 parts; (b) Haematococcus pluvialis extract: 0.05 parts to 5.0 parts; (c) Ambrette coffea fruit extract: 0.5-15.0 parts; (d) Yeast / zinc fermentation product: 0.1 part to 8.0 parts.

2. The composition according to claim 1, characterized in that The weight proportions of the components are as follows: (a) Sphingomonas fermentation product extract: 0.5-5.0 parts; (b) Haematococcus pluvialis extract: 0.1-2.0 parts; (c) Ambrette coffee fruit extract: 1.0-10.0 parts; (d) Yeast / zinc fermentation product: 0.5 parts to 5.0 parts.

3. The composition according to claim 1, characterized in that The sphingomonas fermentation product extract is obtained by fermenting in a culture medium with a carbon-nitrogen ratio greater than 40:1, and then purifying through alcohol precipitation and membrane separation technology.

4. The composition according to any one of claims 1 to 3, characterized in that Used to prepare children's skin care products, the product dosage form is selected from oil-in-water (O / W) emulsion, cream, essence or gel.

5. Use of the composition according to any one of claims 1 to 3 in the preparation of a product that improves the stability of astaxanthin in a cosmetic matrix.

6. Use of the composition according to any one of claims 1 to 3 in the preparation of a skin external use product for enhancing the transdermal absorption efficiency of astaxanthin.

7. Use of the composition according to any one of claims 1 to 3 in the preparation of a hypoallergenic children's skin care product that regulates the microecological balance of children's skin, promotes the relative abundance of Staphylococcus epidermidis, and maintains microbial diversity.

8. A method for improving children's skin condition, characterized in that: The method comprises the step of topically applying the composition according to any one of claims 1 to 3 to the skin.

9. A method for improving the stability of astaxanthin in a topical formulation, characterized in that The method comprises the steps of combining astaxanthin with a synergistic mixture of Sphingomonas ferment extract, Ambrette fruit extract and Saccharomyces / zinc ferment.

10. A method for preparing the composition according to any one of claims 1 to 3, characterized in that: The method comprises the following steps: adding astaxanthin-containing Haematococcus pluvialis extract, Sphingomonas fermentation product extract, Ambrette coffee fruit extract and yeast / zinc fermentation product in the step of cooling the emulsion to below 40°C.

Images