A hydrogel dressing with bacteriostatic repairing effect and a preparation method thereof

Abstract

A hydrogel dressing with bacteriostatic repairing effect comprises the following components in parts by weight: honeysuckle hydrolat 18-22 parts, sanghuang polysaccharide extract 6-8 parts, gelatin 1-3 parts, humectant 2-4 parts and nutrient agent 1-3 parts. The polysaccharide in sanghuang powder is extracted by ultrasonic synergistic cellulase method, cellulase can effectively degrade cellulose in plants, and then effectively break the plant cell wall, so that the plant cell polysaccharide is effectively dissolved, thereby improving the extraction amount of sanghuang polysaccharide. The process is simple, low in price, mild in conditions, and pollution-free to the environment, and is conducive to green industrial production. The sanghuang polysaccharide prepared by the application has strong antibacterial ability and good antioxidant capacity, and is easier to deeply nourish the skin. The weak acid honeysuckle hydrolat with strong anti-inflammatory and antibacterial ability and natural source is used instead of deionized water to prepare cosmetics. The weak acid honeysuckle hydrolat is more suitable for the skin due to its weak acidity, and the special plant fragrance emitted by the weak acid honeysuckle hydrolat can also cheer the body and mind.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of skin care, in particular to a water gel dressing with bacteriostatic and repairing effects and a preparation method thereof. BACKGROUND

[0002] As a traditional Chinese herbal medicine, Sanghuang is known as "forest gold" in the folk, and its medicinal value is recorded in many Chinese medicine books, such as "Bencao Gangmu" which mentioned that Sanghuang can benefit five internal organs, promote the function of intestines and stomach, and remove toxic gas. It is a cold-natured medicine with bitter and astringent taste. Studies have shown that the main active ingredients of Sanghuang include polysaccharides, flavonoids, ergosterol, triterpenoids, etc. (Zhao P, Li CX, Zuo FJ, et al. Research Progress of Chemical Constituents, Pharmacological Activities and Quality Markers (Q-Marker) of Sanghuang [J]. Drug Evaluation and Research), among which Sanghuang polysaccharides, as the core active ingredient, have the effects of anti-tumor, anti-oxidation, anti-sugar, improving immunity, anti-mutation, and scavenging free radicals.

[0003] There are various extraction methods for Sanghuang polysaccharides, such as water extraction, enzymatic hydrolysis, and ultrasonic-assisted extraction, etc. However, as a biological macromolecule, it is difficult to extract and easily inactivated under high temperature conditions, which greatly limits its extraction efficiency. Therefore, it is crucial to optimize the extraction and purification process of Sanghuang polysaccharides through innovative means. In this experiment, ultrasonic-assisted cellulase method was used to extract polysaccharides from Sanghuang powder, which effectively reduced the experimental cost and improved the extraction efficiency by promoting the release of active ingredients under the condition of no harmful chemical reagents added, green and pollution-free, and simplifying the traditional complex process, and was expected to realize industrial production.

[0004] Plant hydrolate is a by-product obtained during the process of distilling essential oil from aromatic plants, which has special plant aroma, contains trace amounts of esters, ketones, alkenes, and alcohols, has hydrophilicity, and most of them are weakly acidic, which can soothe and anti-inflammatory to the skin as cosmetics. Studies have shown that honeysuckle hydrolate is rich in chlorogenic acid, etc., and has good anti-inflammatory and antibacterial ability compared with essential oil (Wang JL, Zhang CF, Xu RY, et al. Functional and Application Research Progress of Active Ingredients of Honeysuckle [J]. Food Safety Guide).

[0005] Water gel has attracted much attention due to its excellent biocompatibility and biomimetic structure. As a wound dressing, it can effectively absorb exudates, prevent infection, and maintain a moist environment, thereby promoting cell adhesion. Gelatin is widely used in the preparation of water gel wound dressings due to its strong moisturizing property, plasticity, and promotion of wound healing. Although the efficacy of Sanghuang polysaccharides in the field of medical care has been widely recognized, its application in medical and cosmetic repair cosmetics with bacteriostatic, antioxidant, and repairing effects is still in the exploratory stage. In particular, the use of Sanghuang polysaccharides to develop natural water gel dressings with these effects is still a technical gap. SUMMARY

[0006] The present application provides a method for preparing a hydrogel dressing with bacteriostatic and repairing functions by using phellinus baumii polysaccharide with excellent antibacterial and antioxidant effects as an effective component.

[0007] The present application belongs to the field of plant extraction and cosmetics, and a skin care product with antioxidant and mild moisturizing effects is prepared by using phellinus baumii polysaccharide and honeysuckle hydrolat as main raw materials. The process is simple, green and environmentally friendly, and fills the gap of phellinus baumii polysaccharide used in the field of cosmetics.

[0008] The present application provides a hydrogel dressing with bacteriostatic and repairing functions, which comprises the following components by weight:

[0009] Honeysuckle hydrolat 18-22 parts

[0010] Phellinus baumii polysaccharide extract 6-8 parts

[0011] Gelatin 1-3 parts

[0012] Moisturizing agent 2-4 parts

[0013] Nutrient agent 1-3 parts.

[0014] Preferably, the phellinus baumii polysaccharide extract is extracted from phellinus baumii powder by ultrasonic synergistic cellulase method, and the extraction rate is 2%~5%.

[0015] Preferably, the preparation method of the phellinus baumii polysaccharide extract comprises the following steps:

[0016] Defatting treatment: phellinus baumii powder is mixed with 90% ethanol at a material-liquid ratio of 1:20~1:30, and refluxed at 80~85℃ for 2~3 h;

[0017] Enzymatic hydrolysis and ultrasonic treatment: defatted phellinus baumii powder is added with water at a solid-liquid ratio of 1:20~1:30, 0.5~1.0 g of biological enzyme is added, enzymatic hydrolysis is carried out at 40~55℃ for 90 min, enzyme inactivation is carried out at 80~100℃ for 5~10 min, and then 300~360 w ultrasonic treatment is carried out for 0.5~2 h;

[0018] Purification: the enzymatic hydrolysate is mixed with 79% ethanol at a volume ratio of 1:5 for precipitation for 10~12 h, and centrifuged and dried to obtain phellinus baumii polysaccharide extract.

[0019] Preferably, in the step of enzymatic hydrolysis and ultrasonic treatment, the defatted phellinus baumii powder is configured into an aqueous solution with a solid-liquid ratio of 1:20~1:30, and the biological enzyme used for enzymatic hydrolysis is at least one of cellulase, papain, pectinase and amylase.

[0020] Preferably, the moisturizing agent is at least one of acetylated sodium hyaluronate, glycerol and polyglutamic acid sodium.

[0021] Preferably, the nutritional agent is selected from at least one of vitamin C, panthenol and nicotinamide.

[0022] Preferably, the preparation method of the honeysuckle hydrolat is as follows: honeysuckle branches and leaves are added with water at a material-to-liquid ratio of 1:2, and then distilled at 120 DEG C for 3 hours, and the lower layer of the condensed liquid is collected to obtain the honeysuckle hydrolat.

[0023] A preparation method of a hydrogel dressing with bacteriostatic repair effect, comprising the following steps:

[0024] S1, weigh the gelatin powder and place it in a container, and then add the honeysuckle hydrolat to it, continuously stir to dissolve the gelatin completely to obtain a gel aqueous solution;

[0025] S2, the phellinus baumii polysaccharide solution, moisturizing agent and nutritional agent are added to the gel aqueous solution and continuously stirred to be uniform, and then cooled to room temperature to form the required hydrogel dressing.

[0026] Preferably, the mass concentration of gelatin in the gel aqueous solution is 10% to 20%, and the stirring temperature is 40 to 50 DEG C.

[0027] Advantages of the present application:

[0028] The present application uses ultrasonic synergistic cellulase method to extract polysaccharides from phellinus baumii powder, utilizes cellulase to effectively degrade cellulose in plants, and then effectively breaks the plant cell wall, so that the polysaccharides in the plant cells are effectively dissolved, thereby improving the extraction amount of phellinus baumii polysaccharides. This process is simple, low in price, mild in conditions, and pollution-free to the environment, which is conducive to green industrial production. The phellinus baumii polysaccharides prepared by the present application not only have strong antibacterial ability, but also have good antioxidant capacity, and are more easily to deeply nourish the skin. The weak acid honeysuckle hydrolat with strong anti-inflammatory and antibacterial ability and natural source is used instead of deionized water to prepare cosmetics. Not only does it fit the skin better because of its weak acidity, but also the special plant fragrance emitted by itself can also bring joy to the body and mind. Therefore, the phellinus baumii polysaccharides and the honeysuckle hydrolat are used together to prepare a hydrogel dressing with bacteriostatic repair effect, which is not only mild and non-irritating, but also can effectively inhibit the activity of active free radicals, is suitable for emergency relief of damaged skin, and is suitable for long-term anti-aging and antioxidant. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1 The infrared spectrum of the phellinus baumii polysaccharide extract.

[0030] Figure 2 The swelling ratio schematic diagram of the hydrogel dressing. DETAILED DESCRIPTION

[0031] In order to make the purposes, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be described clearly and completely below in conjunction with the embodiments of the present application. Obviously, the described embodiments are some but not all of the embodiments of the present application.

[0032] <Embodiment 1>

[0033] (1) Preparation of Phellinus baumii polysaccharide:

[0034] Take 5.0 g of Phellinus baumii powder in a 250 mL round-bottom flask, add 125 mL of 90% ethanol solution, and place it in an 80°C oil bath for two hours. In this process, the lipids in the Phellinus baumii powder will be dissolved in ethanol. After two hours, the solution in the flask is filtered, and then 125 mL of 90% ethanol solution is added again, and the above operation is repeated to completely dissolve the lipids in the Phellinus baumii powder. The defatted Phellinus baumii powder is obtained by filtration, and is placed in a 60°C vacuum drying oven to dry to constant weight. The defatted Phellinus baumii powder is sealed in a self-sealing bag for storage.

[0035] Take 3.0 g of defatted Phellinus baumii powder and add 80 mL of deionized water, and add 0.5 g of cellulase. The reaction temperature is 45°C, and the extraction time is 90 min. Then, the temperature is raised to 80°C, and the enzyme is inactivated for 5 min. Under the conditions of a temperature of 80°C and a power of 360 w, ultrasonic treatment is carried out for 1 h. After the temperature drops to 50°C, the pH of the system is adjusted to 4. Then, the above solution is centrifuged. Since the polysaccharide to be extracted is dissolved in water, the supernatant is retained. In order to completely remove the residue, the supernatant after centrifugation is filtered again, and the filtrate is retained. The filtrate is then concentrated to about 20 mL by rotary evaporation. Since the polysaccharide is insoluble in ethanol, 79% ethanol can be added to the extract to precipitate the polysaccharide in the extract. The volume ratio of the Phellinus baumii concentrate to ethanol is 1:5. After standing for 12 h, the precipitate is completely precipitated, and the alcohol-sedimented solution is centrifuged. The precipitate is obtained, and finally the precipitate is freeze-dried to obtain the Phellinus baumii polysaccharide extract. The infrared spectrum is shown in Figure 1 Table 1 shows other extraction methods.

[0036] Table 1

[0037]

[0038] (2) Preparation of honeysuckle hydrol:

[0039] Fresh 3 kg of honeysuckle branches and leaves are cut into segments and placed in a distillation flask. According to the solid-liquid ratio of 1:2, 6 kg of deionized water is added. The distillation temperature is set to 120°C. When the water starts to boil and produce water vapor, the condensation device is turned on. The heating temperature is set to 100°C. After 3 h, the aromatic substances distilled out are cooled and placed in a collection device. The lower layer of liquid collected is the honeysuckle hydrol.

[0040] (3) Preparation of finished product:

[0041] The following substances were added according to the mass ratio. 2 parts of gelatin with a concentration of 10% were added to 20 parts of honeysuckle hydrolat prepared in step (2) to prepare a gelatin aqueous solution at 50°C as phase A for standby. 7 parts of the polysaccharide prepared in step (1) were added to phase A, followed by 3 parts of the moisturizing agent sodium acetylated hyaluronic acid and 2 parts of the nutrient vitamin C, and stirred uniformly. Until the product cooled to room temperature, it was coagulated into a finished product sample.

[0042] [TESTING EXPERIMENT]

[0043] (1) Antibacterial performance experiment

[0044] 160 μL of the hydrogel prepolymer solution was spread in a 48-well plate, blue light was irradiated for 30 min to form a hydrogel scaffold. Then 10 5 CFU / L of Staphylococcus aureus bacterial suspension was prepared. 70 μL of the bacterial suspension was spread on the M-H plate, and the hydrogel scaffold was spread on the M-H plate, and incubated at a constant temperature of 37°C for 1 d. The inhibition zone on the plate was observed and measured. As shown in the table, the sample has good inhibition effect on Staphylococcus aureus, as shown in Table 2.

[0045] Table 2

[0046]

[0047] (2) Hydrogel performance test experiment (swelling ratio experiment)

[0048] The sample was immersed in a phosphate buffer solution and placed in a constant temperature environment of 37°C for 2 d. Then it was taken out, the excess phosphate buffer solution on the surface of the material was absorbed, weighed and recorded as V1. Then the sample was dried and weighed and recorded as V2. Then the swelling ratio of the hydrogel was calculated, the formula was:

[0049]

[0050] The experimental results are shown in Figure 2 , and the results show that the hydrogel swelling ratio of the sample is good.

[0051] (3) Wound healing

[0052] Wound healing: According to the wound healing treatment and time of the patients in the facial skin defect repair treatment surgery, the wound healing was evaluated, which was divided into three levels of excellent, good and poor. And the excellent and good rate of wound healing was calculated. Wound scar proliferation: According to the Vancouver Scar Scale, the wound scar proliferation of each group of patients at a certain time after surgery was evaluated by VSS. The evaluation used Linker six-level score, that is, 0-5 points. The higher the VSS score, the more serious the wound scar proliferation of the patients at a certain time after surgery. Patient satisfaction: A questionnaire was used to access the satisfaction of the patients 6 months after the facial skin defect repair treatment surgery. The total score was set to 10 points, of which less than 6 points was not satisfied, 6-7 points was satisfied, and 8-10 points was very satisfied. The patient satisfaction was calculated. In this study, all data were processed using SPSS 25.0 software, and "P<0.05" indicated that there was a statistically significant difference between the groups. 92 patients who underwent facial skin defect repair treatment were selected and randomly divided into control and experimental groups, with 46 patients in each group. The inclusion criteria for patients were: (1) knowing and voluntarily agreeing to participate in this study; (2) patients aged 18-60 years; (3) patients received facial surgery within 6 hours of facial injury; (4) all patients had facial skin defects. The exclusion criteria for patients were: (1) patients who had received the same surgery; (2) patients with mental or infectious diseases; (3) patients with severe abnormalities in heart, liver and kidney function. In the facial skin defect repair treatment, the patients in the control group were treated by ordinary skin flap repair, and the patients in the experimental group were treated by hydrogel dressing. There was no difference in dressing change date and bandaging method between the two groups.

[0053] The experimental results are shown in Table 3 below. The wound healing of the hydrogel sample was better.

[0054] Table 3

[0055]

[0056] (4) Skin irritation

[0057] The test was carried out using 0.5 mL of the hydrogel solution according to the multiple intact skin irritation test method in the Disinfection Technical Standard (2002 Edition). 24 h before the test, the fur on both sides of the dorsal spine of the guinea pig was removed without damaging the skin. The fur was removed in an area of about 3 cm x 3 cm on the left and right. During the test, 0.5 mL of the hydrogel solution was directly dropped on 2-4 layers of gauze with an area of 2.5 cm x 2.5 cm and applied to the surface of the left fur-removed skin, then covered with a layer of non-irritating plastic film and fixed with non-irritating adhesive tape. 0.5 mL of 0.9% sodium chloride injection was applied to the right side as a control. After 4 h of application, the residual was removed by washing with warm water. Application was carried out once a day for 14 d, and the test material was applied for 4 h each time, and the residual test material was washed with water or a non-irritating appropriate solvent. The skin of the animals was observed for erythema and edema 24 h after the application of the test material, and scored according to the scoring standard for skin irritation response. It was found that the hydrogel had no irritation to the multiple intact skin of guinea pigs, meeting the requirements of the Disinfection Technical Standard (2002 Edition).

[0058] The experimental results are shown in Table 4 below, and the sample is almost non-irritating.

[0059] Table 4

[0060]

[0061] The above examples are only for illustrating the technical solutions and characteristics of the present application, and the purpose is to better enable those skilled in the art to implement them, and cannot limit the protection scope of the present application. Any equivalent changes or modifications made according to the spirit and essence of the present application are within the protection scope of the present application, and those not described in detail are prior art.

Claims

1. A hydrogel dressing having bacteriostatic healing effect, characterized in that, The following components are included by weight parts: 18-22 parts of honeysuckle hydrolat 6-8 parts of polysaccharide extract of Phellinus baumii 1-3 parts of gelatin 2-4 parts of moisturizing agent 1-3 parts of nutrient agent The preparation method of the hydrogel dressing includes the following steps: S1. Weigh the gelatin powder into a container and add honeysuckle hydrolat to it, continuously stir until the gelatin is completely dissolved to obtain a gel aqueous solution; S2. Add the polysaccharide solution of Phellinus baumii, moisturizing agent, and nutrient agent to the gel aqueous solution and continue to stir until uniform, then cool to room temperature to form the desired hydrogel dressing; The gelatin concentration in the gel aqueous solution is 10%~20%, and the stirring temperature is 40~50℃.

2. The hydrogel dressing with bacteriostatic repairing effect according to claim 1, characterized in that, The polysaccharide extract of Phellinus baumii is extracted from Phellinus baumii powder by ultrasonic-assisted cellulase method, with an extraction rate of 2%~5%.

3. The hydrogel dressing with bacteriostatic repairing effect according to claim 2, characterized in that, The preparation method of the polysaccharide extract of Phellinus baumii includes the following steps: Defatting treatment: mix Phellinus baumii powder with 90% ethanol at a material-to-liquid ratio of 1:20~1:30, and reflux at 80~85℃ for 2~3 h; Enzymatic hydrolysis and ultrasonication: add 0.5~1.0 g of biological enzyme to defatted Phellinus baumii powder at a solid-to-liquid ratio of 1:20~1:30, and hydrolyze at 40~55℃ for 90 min, then inactivate the enzyme at 80~100℃ for 5~10 min, and then ultrasonicate at 300~360 w for 0.5~2 h; Purification: mix the enzymatic hydrolysate with 79% ethanol at a volume ratio of 1:5, precipitate for 10~12 h, and dry by centrifugation to obtain the polysaccharide extract of Phellinus baumii.

4. The hydrogel dressing with bacteriostatic repairing effect according to claim 3, characterized in that, In the enzymatic hydrolysis and ultrasonication step, the defatted Phellinus baumii powder is configured into an aqueous solution at a solid-to-liquid ratio of 1:20~1:30, and the biological enzyme used for enzymatic hydrolysis is at least one of cellulase, papain, pectinase, and amylase.

5. The hydrogel dressing with bacteriostatic repairing effect according to claim 1, characterized in that, The moisturizing agent is selected from at least one of acetylated sodium hyaluronate, glycerol, and sodium polyglutamate.

6. The hydrogel dressing with bacteriostatic repairing effect according to claim 1, characterized in that, The nutrient agent is selected from at least one of vitamin C, panthenol, and nicotinamide.

7. The hydrogel dressing with bacteriostatic repairing effect according to claim 1, characterized in that, The preparation method of honeysuckle hydrolat is as follows: add water to honeysuckle branches and leaves at a material-to-liquid ratio of 1:2, and distill at 120℃ for 3 h, then collect the lower condensate to obtain honeysuckle hydrolat.

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